CsGy6G032920 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy6G032920
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Descriptionaspartic proteinase-like
LocationGy14Chr6: 29921101 .. 29925416 (+)
RNA-Seq ExpressionCsGy6G032920
SyntenyCsGy6G032920
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGACGGTTCTGATCGAAAGCTCCAACCAGAAATCCATGGAATGAAAGCCATTGCAAAAACCCACCAAGAAATGGAATTTCTACGTTCGGGCCCTTTTGGTAAGTGGGTCCCTTTTGTTCAAAGGAATGGAGTGAAGTGACAGCTTAAAATGCCACTCTTTTCCTTTCTTCAACTCCTATTTAAACCTCATTTGATGTTCCCATTTACCTTCAACCCTTTCTCCTTACTTCTCTTCTAGAAATCTGTTTCATTTGGTGGGTTAGTATTGATCTTTTCTTTTGTATTTGTTTTGGGTTCCTTCAGAATTGTATATGATCATCAAATTTTGATTCATTTATTTGGTTTTTTTCTTTGCTATGTTTGTGGTTATCACCCTTTATTTCATGTTTCTGGATGAAATGGATTGCAGTTAACAGTCAGTTTAAAGAAAGTGAGTTACTGAATCAATGGTCCTGATTTGTCAGTATTTAGAAAAGGAATTGTTGTGTGTATGTGTTTCTTTTTTGGGTTTGGTATATTGTTGATTTCTCACGAGGATAAAACTATGGACTTCCCATTTATTCTTTTTTTCCTCCCTTTTAGCTTTCTAACTTTAAGTCTAAAGTTCTCTTAAAGCTAAAAGTCGTAGGGAACCTTTTCTTTTATGTTTCTTCCAGCTCCATTTTTCTGAGCTTTAAAGCCTTCTATTTGTGCTTGTAGGTTACAATGAGAAAGATCAGCTTTAGTCACCTTTTGGTTTCTCTGTTGCTTTTGATTCTACATTATTCCTCTGAAGCAACATCTGCTTCCAATGAAGGGTTTTTAAGGATTGGATTGAAAAAGATTAAATATGATCAAAACAGTCGGTTCAAAGCTTTGCTTGAGTCAAAGAAAGGAGAGTTTTTAGGATCTTCTGTTGGAAAACATAATCAATGGGGTAATAATCTTGAAGAATCTAAAAATGCTGATATTGTACCATTAAAGAACTATTTGGATGCTCAATACTATGGAGAGATTGGCATTGGCACACCACCTCAAAAGTTCACTGTAATTTTTGATACTGGAAGCTCTAATTTGTGGGTGCCATCTGCCAAATGTATTTTTTCGGTGAGGATTACTTAAACTCTAATCAAGAAAGAAGAATAATATGATGATTCGTTTGTGACTAGTAATATGTTTGTTTTACCTTCACATGCAGCTTGCTTGCTTTTTCCATGCTAAGTATCAATCAGGGCGGTCAAGCACATACAAAAGAAATGGTATGTTTTTCTTCTCTTTACCTGCAACAGTCTTCAAAATGAAACTTTGTTCCCTGCATTCAAATGAGTTTGATTAATCTTATTTCATTTATCCGCATATTAGATTAGTCAAATATCGAAAGATGATGTTTAGGAACTAGTAAGTGGCTTTCATGAAGTGAGTTATTGTATTTTATTTGTTCTTGCTATCACTGTAAGGCTAGTCGCTTATGGTCGAATGTGTCTTCCATCTCATTCTGGTAGAGATAAGAAACATCTGCTGCTATTCAGTACGGTTGTAAAGTTGTGTTTTATATTACACAGGAACATCTGCTGCTATTCAGTACGGTTCAGGAGCTATTTCTGGCTTCTTTAGTTACGACAACGTTCAAGTTGGTGATGTTATCGTTCGTAATCAGGTAATCTTACCTTTTTTTTTGGTTTGAAGTTTGAGAAGGCAATTAGTTGTATCTAACATTGATTAAATTCAGGAACTCATTGAGGCAACTAGCATGTCTACTATGACATTCATGGCCGCCAAATTTGATGGTATATTGGGACTTGGATTTCAAGAGATCGCGACTGGTGGTGCTGTTCCAGTGTGGTATGCTAAATGCAACTATAATACTAGTTGCTGCTTGCTGAAGAAGTTCTTTAAGAAATTTTAATCAGGCTTTTGATTGCCCCTTAGGTATAACATGGTTAAACAAAAACTTGTCAAGGAGCAAGTTTTTTCATTTTGGCTGAATCGTAATGCCGAAGAGAAAGAAGGAGGTGAGCTTGTGTTTGGAGGGGTCGATCCTAAGCACTTCAAAGGCCAACATACATATGTGCCTGTGACTGACAAAGGGTATTGGCAGGTTGGCTCTTGAAATCCTTTCAACACTGAGCATTACTTTAAATTTTGCCTTTATCCTCACACAGTTCAAATGCGTTTTCTTGTAGTTCGACATTGGCGATATTCTTATTGGTGGTGAAACAACAAGTATAATCCCTTTACTTATCTAGGCTTTTGAGTGTTATTGTCACATCATGTATAACATGCCTCTGACTAACGTATACAACATGATATACAGAATATTGTGCTGGCGGTTGCTCTGCAATTGCAGATTCTGGAACTTCTTTGTTGGCTGGTCCATCTGTATGTACACAAATTATTTTAGTTTATTTTCTTTATCTCTCTTTTATCCACATGAGGGTCAGGTTGATAAAGAAACAACATGAAGTTGAGAAATGAGAAGTAGTTGATTAATTTGGAAGAATGTAAACATGTTTGTTTTCTGTAAACTTTTGCTCCAATAACAACCTTTACATCATGTAACCGATAACTGAAGGGTTCCCTTTATTAACTTAGCATATAAGTGGTCCTTCACTAATCGTACATGATGAAGGCTAGCAACCTCCGTAGCTTATGCACTTTATTAGAGAATATTTCTTCTCCATTGCAGAATATAGTGGTATCAATTAATAGAGCAATTGGAGCAGCTGCAGTTGCACATCCAGAATGCAAGGCAATTGTTTCACAATATGGACGGGCTATTATGGATTTGCTTTTAGCCAAGGTAAAACATGCCCTGTACTTTTTTTCTCACAATGTAATCTATCCTTAACAACCAGATTAAGGAGTTGTTTTTACATTCTAATGGCAGGCACAACCAGAGAAGATATGCTCCAAAATTGGGGTGTGTACCTTTGATGAAACTCATGATGTTAGGTTAGTCTAATGCTTCAACAAGATGAGTCATTTTTCAGCAATTCTACTAACTACTTTTCCTTTCAGTTGCATAAAAGATCAGTTTTATTAACTTACGGTTGGTAATTGAAACCAGTTTGAAAATTGAGAACGTGGTGAGTGATAAAGATGGTAGATCGTCTGGTGGGTTCTCAGAAGCTATGTGCTCAGCTTGTGAGATGGCGGTTTTGTGGATTCAGGATGAGCTGAAGCAGAACAAAACTCAAGAAGATATCATTGAGAATGTCAATGAGGTATACTAGTTCTGTTTCCATATAACTCATTAGTATACCTCATCCACAGATGATTCATCTTCAGCTGACTTGCCTTTCGTGTTTCTGCAGCTATGCGACCGTGGGTTGAACCAAGACGAAACATTGGTTGACTGTGGACGAATCTCTCAAATGCCTAACGTGTCCTTCACCATTGGCGATAGACTTTTTGAGCTCACCTCAAAAGATGTATTTTTCTTCCCAAATCTTTCTTTACAACTCCTGACTTTGTTTCATACTTGAAGAATATAATATGTCAGTGATTAGTTGTCAATCACGAATAACTTGGTAGGAGAGATCTAATGCGTCTTTGTGCTCAATTATTCTTCTGTCAAATACTCTGTCATCTGGTGCTCGAAGGCATCCTTTCCTTGTCATATGATGTCGTTTCACGTTCTGTGCTTTTGACCCTAACCAGTGCTTCAAGTATCTTATGCCGCCAACATCTTAGCCTTATTCATTCTAACCTCATTGTTGTTTGTGAACAGTATATTCTGAAGGTGGGTGAGGGATCTGCAGCTCAATGCATCAGTGGATTCATACCTTTCGACATTCCTCCTCCTCGTGGACCCCTATGGTATTTCCATTTTCATGTTCTTGTTTTCTTGCCTCTCTTTAAATCTCAGAGTCGTGTCTCTTTAAGATAAAGATTCTTCCCACTCGTTTATTTTTACCGGTGAAATAAACGTGCTGACAATGCAGGATCTTGGGAGACGTTTTCATGGGACCTTATCATACAGTCTTTGATTTTGGCAAAGCGCGAGTCGGATTTGCTGAAGCTGCTTGAAGAACATGTATACTCAGGACTTGAATGGTGACTCTTGTGTGTATACATAAGTAATGAAGTTGTTCATAATAAAGAGAGAGAGAGAGAGAGAGAAAGAGAGCACCTGTAAATGTTGTGGTGTTTAAAATATCATTTCTATAATCTTAGTAATTAGTGGAAACTTTCATTAAAAATTGTTTTCTCAGTTTATTCTTCTCATCTTTCTTATACAACTAAATGTCATAGTACTCTATAGTAACATATAAAAGATTACACTTCTTTGGTAGTCAACGATTTCACTGGTAGACTCAAAATTTAATAGAAAATTC

mRNA sequence

CGACGGTTCTGATCGAAAGCTCCAACCAGAAATCCATGGAATGAAAGCCATTGCAAAAACCCACCAAGAAATGGAATTTCTACGTTCGGGCCCTTTTGGTAAGTGGGTCCCTTTTGTTCAAAGGAATGGAGTGAAGTGACAGCTTAAAATGCCACTCTTTTCCTTTCTTCAACTCCTATTTAAACCTCATTTGATGTTCCCATTTACCTTCAACCCTTTCTCCTTACTTCTCTTCTAGAAATCTGTTTCATTTGGTGGGTTACAATGAGAAAGATCAGCTTTAGTCACCTTTTGGTTTCTCTGTTGCTTTTGATTCTACATTATTCCTCTGAAGCAACATCTGCTTCCAATGAAGGGTTTTTAAGGATTGGATTGAAAAAGATTAAATATGATCAAAACAGTCGGTTCAAAGCTTTGCTTGAGTCAAAGAAAGGAGAGTTTTTAGGATCTTCTGTTGGAAAACATAATCAATGGGGTAATAATCTTGAAGAATCTAAAAATGCTGATATTGTACCATTAAAGAACTATTTGGATGCTCAATACTATGGAGAGATTGGCATTGGCACACCACCTCAAAAGTTCACTGTAATTTTTGATACTGGAAGCTCTAATTTGTGGGTGCCATCTGCCAAATGTATTTTTTCGCTTGCTTGCTTTTTCCATGCTAAGTATCAATCAGGGCGGTCAAGCACATACAAAAGAAATGGAACATCTGCTGCTATTCAGTACGGTTCAGGAGCTATTTCTGGCTTCTTTAGTTACGACAACGTTCAAGTTGGTGATGTTATCGTTCGTAATCAGGAACTCATTGAGGCAACTAGCATGTCTACTATGACATTCATGGCCGCCAAATTTGATGGTATATTGGGACTTGGATTTCAAGAGATCGCGACTGGTGGTGCTGTTCCAGTGTGGTATAACATGGTTAAACAAAAACTTGTCAAGGAGCAAGTTTTTTCATTTTGGCTGAATCGTAATGCCGAAGAGAAAGAAGGAGGTGAGCTTGTGTTTGGAGGGGTCGATCCTAAGCACTTCAAAGGCCAACATACATATGTGCCTGTGACTGACAAAGGGTATTGGCAGTTCGACATTGGCGATATTCTTATTGGTGGTGAAACAACAAAATATTGTGCTGGCGGTTGCTCTGCAATTGCAGATTCTGGAACTTCTTTGTTGGCTGGTCCATCTAATATAGTGGTATCAATTAATAGAGCAATTGGAGCAGCTGCAGTTGCACATCCAGAATGCAAGGCAATTGTTTCACAATATGGACGGGCTATTATGGATTTGCTTTTAGCCAAGGCACAACCAGAGAAGATATGCTCCAAAATTGGGGTGTGTACCTTTGATGAAACTCATGATGTTAGTTTGAAAATTGAGAACGTGGTGAGTGATAAAGATGGTAGATCGTCTGGTGGGTTCTCAGAAGCTATGTGCTCAGCTTGTGAGATGGCGGTTTTGTGGATTCAGGATGAGCTGAAGCAGAACAAAACTCAAGAAGATATCATTGAGAATGTCAATGAGCTATGCGACCGTGGGTTGAACCAAGACGAAACATTGGTTGACTGTGGACGAATCTCTCAAATGCCTAACGTGTCCTTCACCATTGGCGATAGACTTTTTGAGCTCACCTCAAAAGATTATATTCTGAAGGTGGGTGAGGGATCTGCAGCTCAATGCATCAGTGGATTCATACCTTTCGACATTCCTCCTCCTCGTGGACCCCTATGGATCTTGGGAGACGTTTTCATGGGACCTTATCATACAGTCTTTGATTTTGGCAAAGCGCGAGTCGGATTTGCTGAAGCTGCTTGAAGAACATGTATACTCAGGACTTGAATGGTGACTCTTGTGTGTATACATAAGTAATGAAGTTGTTCATAATAAAGAGAGAGAGAGAGAGAGAGAAAGAGAGCACCTGTAAATGTTGTGGTGTTTAAAATATCATTTCTATAATCTTAGTAATTAGTGGAAACTTTCATTAAAAATTGTTTTCTCAGTTTATTCTTCTCATCTTTCTTATACAACTAAATGTCATAGTACTCTATAGTAACATATAAAAGATTACACTTCTTTGGTAGTCAACGATTTCACTGGTAGACTCAAAATTTAATAGAAAATTC

Coding sequence (CDS)

ATGAGAAAGATCAGCTTTAGTCACCTTTTGGTTTCTCTGTTGCTTTTGATTCTACATTATTCCTCTGAAGCAACATCTGCTTCCAATGAAGGGTTTTTAAGGATTGGATTGAAAAAGATTAAATATGATCAAAACAGTCGGTTCAAAGCTTTGCTTGAGTCAAAGAAAGGAGAGTTTTTAGGATCTTCTGTTGGAAAACATAATCAATGGGGTAATAATCTTGAAGAATCTAAAAATGCTGATATTGTACCATTAAAGAACTATTTGGATGCTCAATACTATGGAGAGATTGGCATTGGCACACCACCTCAAAAGTTCACTGTAATTTTTGATACTGGAAGCTCTAATTTGTGGGTGCCATCTGCCAAATGTATTTTTTCGCTTGCTTGCTTTTTCCATGCTAAGTATCAATCAGGGCGGTCAAGCACATACAAAAGAAATGGAACATCTGCTGCTATTCAGTACGGTTCAGGAGCTATTTCTGGCTTCTTTAGTTACGACAACGTTCAAGTTGGTGATGTTATCGTTCGTAATCAGGAACTCATTGAGGCAACTAGCATGTCTACTATGACATTCATGGCCGCCAAATTTGATGGTATATTGGGACTTGGATTTCAAGAGATCGCGACTGGTGGTGCTGTTCCAGTGTGGTATAACATGGTTAAACAAAAACTTGTCAAGGAGCAAGTTTTTTCATTTTGGCTGAATCGTAATGCCGAAGAGAAAGAAGGAGGTGAGCTTGTGTTTGGAGGGGTCGATCCTAAGCACTTCAAAGGCCAACATACATATGTGCCTGTGACTGACAAAGGGTATTGGCAGTTCGACATTGGCGATATTCTTATTGGTGGTGAAACAACAAAATATTGTGCTGGCGGTTGCTCTGCAATTGCAGATTCTGGAACTTCTTTGTTGGCTGGTCCATCTAATATAGTGGTATCAATTAATAGAGCAATTGGAGCAGCTGCAGTTGCACATCCAGAATGCAAGGCAATTGTTTCACAATATGGACGGGCTATTATGGATTTGCTTTTAGCCAAGGCACAACCAGAGAAGATATGCTCCAAAATTGGGGTGTGTACCTTTGATGAAACTCATGATGTTAGTTTGAAAATTGAGAACGTGGTGAGTGATAAAGATGGTAGATCGTCTGGTGGGTTCTCAGAAGCTATGTGCTCAGCTTGTGAGATGGCGGTTTTGTGGATTCAGGATGAGCTGAAGCAGAACAAAACTCAAGAAGATATCATTGAGAATGTCAATGAGCTATGCGACCGTGGGTTGAACCAAGACGAAACATTGGTTGACTGTGGACGAATCTCTCAAATGCCTAACGTGTCCTTCACCATTGGCGATAGACTTTTTGAGCTCACCTCAAAAGATTATATTCTGAAGGTGGGTGAGGGATCTGCAGCTCAATGCATCAGTGGATTCATACCTTTCGACATTCCTCCTCCTCGTGGACCCCTATGGATCTTGGGAGACGTTTTCATGGGACCTTATCATACAGTCTTTGATTTTGGCAAAGCGCGAGTCGGATTTGCTGAAGCTGCTTGA

Protein sequence

MRKISFSHLLVSLLLLILHYSSEATSASNEGFLRIGLKKIKYDQNSRFKALLESKKGEFLGSSVGKHNQWGNNLEESKNADIVPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSAKCIFSLACFFHAKYQSGRSSTYKRNGTSAAIQYGSGAISGFFSYDNVQVGDVIVRNQELIEATSMSTMTFMAAKFDGILGLGFQEIATGGAVPVWYNMVKQKLVKEQVFSFWLNRNAEEKEGGELVFGGVDPKHFKGQHTYVPVTDKGYWQFDIGDILIGGETTKYCAGGCSAIADSGTSLLAGPSNIVVSINRAIGAAAVAHPECKAIVSQYGRAIMDLLLAKAQPEKICSKIGVCTFDETHDVSLKIENVVSDKDGRSSGGFSEAMCSACEMAVLWIQDELKQNKTQEDIIENVNELCDRGLNQDETLVDCGRISQMPNVSFTIGDRLFELTSKDYILKVGEGSAAQCISGFIPFDIPPPRGPLWILGDVFMGPYHTVFDFGKARVGFAEAA*
Homology
BLAST of CsGy6G032920 vs. ExPASy Swiss-Prot
Match: O04057 (Aspartic proteinase OS=Cucurbita pepo OX=3663 PE=2 SV=1)

HSP 1 Score: 691.8 bits (1784), Expect = 5.9e-198
Identity = 334/505 (66.14%), Postives = 408/505 (80.79%), Query Frame = 0

Query: 13  LLLLILHYSSEATSASNEGFLRIGLKKIKYDQNSRFKALLESKKGEFLGSSVGKHNQWGN 72
           L L +L   +  +SASN+G LR+GLKKIK D  +R  A +ESK  E L ++  K+N  G 
Sbjct: 11  LCLFLLVSFNIVSSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKAAFRKYNPKG- 70

Query: 73  NLEESKNADIVPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSAKCIFSLACFF 132
           NL ES + DIV LKNYLDAQYYGEI IGTPPQKFTVIFDTGSSNLWV   +C+FS+AC F
Sbjct: 71  NLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWV-LCECLFSVACHF 130

Query: 133 HAKYQSGRSSTYKRNGTSAAIQYGSGAISGFFSYDNVQVGDVIVRNQELIEATSMSTMTF 192
           HA+Y+S RSS+YK+NGTSA+I+YG+GA+SGFFSYDNV+VGD++V+ Q  IEAT   ++TF
Sbjct: 131 HARYKSSRSSSYKKNGTSASIRYGTGAVSGFFSYDNVKVGDLVVKEQVFIEATREPSLTF 190

Query: 193 MAAKFDGILGLGFQEIATGGAVPVWYNMVKQKLVKEQVFSFWLNRNAEEKEGGELVFGGV 252
           + AKFDG+LGLGFQEIA G AVPVWYNMV+Q LVKE VFSFWLNRN EE+EGGE+VFGGV
Sbjct: 191 LVAKFDGLLGLGFQEIAVGNAVPVWYNMVEQGLVKEPVFSFWLNRNVEEEEGGEIVFGGV 250

Query: 253 DPKHFKGQHTYVPVTDKGYWQFDIGDILIGGETTKYCAGGCSAIADSGTSLLAGPSNIVV 312
           DPKH++G+HTYVPVT KGYWQFD+GD+LI GE T +C GGCSAIADSGTSLLAGP+ ++ 
Sbjct: 251 DPKHYRGKHTYVPVTQKGYWQFDMGDVLIDGEPTGFCDGGCSAIADSGTSLLAGPTPVIT 310

Query: 313 SINRAIGAAAVAHPECKAIVSQYGRAIMDLLLAKAQPEKICSKIGVCTFDETHDVSLKIE 372
            IN AIGA  V   +CKA+V+QYG+ IMDLLL++A P+KICS+I +CTFD T  VS+ IE
Sbjct: 311 MINHAIGAKGVVSQQCKAVVAQYGQTIMDLLLSEADPKKICSQINLCTFDGTRGVSMGIE 370

Query: 373 NVVSDKDGRSSGGFSEAMCSACEMAVLWIQDELKQNKTQEDIIENVNELCDRGLN-QDET 432
           +VV +  G+SS    + MCS CEM V+W+Q++L+QN+T+E II  +NELCDR  +   ++
Sbjct: 371 SVVDENAGKSSDSLHDGMCSVCEMTVVWMQNQLRQNQTKERIINYINELCDRMPSPMGQS 430

Query: 433 LVDCGRISQMPNVSFTIGDRLFELTSKDYILKVGEGSAAQCISGFIPFDIPPPRGPLWIL 492
            VDCG++S MP VSFTIG ++F+L  ++YILKVGEG  AQCISGF  FDIPPPRGPLWIL
Sbjct: 431 AVDCGQLSSMPTVSFTIGGKIFDLAPEEYILKVGEGPVAQCISGFTAFDIPPPRGPLWIL 490

Query: 493 GDVFMGPYHTVFDFGKARVGFAEAA 517
           GDVFMG YHTVFDFGK RVG AEAA
Sbjct: 491 GDVFMGRYHTVFDFGKLRVGSAEAA 513

BLAST of CsGy6G032920 vs. ExPASy Swiss-Prot
Match: Q8VYL3 (Aspartic proteinase A2 OS=Arabidopsis thaliana OX=3702 GN=APA2 PE=2 SV=1)

HSP 1 Score: 677.9 bits (1748), Expect = 8.8e-194
Identity = 328/516 (63.57%), Postives = 406/516 (78.68%), Query Frame = 0

Query: 2   RKISFSHLLVSLLLLILHYSSEATSASNEGFLRIGLKKIKYDQNSRFKALLESKKGEFLG 61
           R ++FS + VS LL    YS       N+G  R+GLKK+K D N+R      SK+ E L 
Sbjct: 6   RAVAFS-VFVSFLLFFTAYSKR-----NDGTFRVGLKKLKLDPNNRLATRFGSKQEEALR 65

Query: 62  SSVGKHNQWGNNL-EESKNADIVPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVP 121
           SS+  +N   NNL  +S +ADIVPLKNYLDAQYYGEI IGTPPQKFTVIFDTGSSNLWVP
Sbjct: 66  SSLRSYN---NNLGGDSGDADIVPLKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVP 125

Query: 122 SAKCIFSLACFFHAKYQSGRSSTYKRNGTSAAIQYGSGAISGFFSYDNVQVGDVIVRNQE 181
           S KC FSL+C+FHAKY+S RSSTYK++G  AAI YGSG+ISGFFSYD V VGD++V++QE
Sbjct: 126 SGKCFFSLSCYFHAKYKSSRSSTYKKSGKRAAIHYGSGSISGFFSYDAVTVGDLVVKDQE 185

Query: 182 LIEATSMSTMTFMAAKFDGILGLGFQEIATGGAVPVWYNMVKQKLVKEQVFSFWLNRNAE 241
            IE TS   +TF+ AKFDG+LGLGFQEIA G A PVWYNM+KQ L+K  VFSFWLNR+ +
Sbjct: 186 FIETTSEPGLTFLVAKFDGLLGLGFQEIAVGNATPVWYNMLKQGLIKRPVFSFWLNRDPK 245

Query: 242 EKEGGELVFGGVDPKHFKGQHTYVPVTDKGYWQFDIGDILIGGETTKYCAGGCSAIADSG 301
            +EGGE+VFGGVDPKHF+G+HT+VPVT +GYWQFD+G++LI GE+T YC  GCSAIADSG
Sbjct: 246 SEEGGEIVFGGVDPKHFRGEHTFVPVTQRGYWQFDMGEVLIAGESTGYCGSGCSAIADSG 305

Query: 302 TSLLAGPSNIVVSINRAIGAAAVAHPECKAIVSQYGRAIMDLLLAKAQPEKICSKIGVCT 361
           TSLLAGP+ +V  IN+AIGA+ V   +CK +V QYG+ I+DLLLA+ QP+KICS+IG+C 
Sbjct: 306 TSLLAGPTAVVAMINKAIGASGVVSQQCKTVVDQYGQTILDLLLAETQPKKICSQIGLCA 365

Query: 362 FDETHDVSLKIENVVSDKDGRSSGGFSEAMCSACEMAVLWIQDELKQNKTQEDIIENVNE 421
           +D TH VS+ IE+VV  ++ RSS G  +A C ACEMAV+WIQ +L+QN TQE I+  +NE
Sbjct: 366 YDGTHGVSMGIESVVDKENTRSSSGLRDAGCPACEMAVVWIQSQLRQNMTQERIVNYINE 425

Query: 422 LCDRGLNQD-ETLVDCGRISQMPNVSFTIGDRLFELTSKDYILKVGEGSAAQCISGFIPF 481
           +C+R  + + E+ VDC ++S+MP VSFTIG ++F+L  ++Y+LK+GEG  AQCISGF   
Sbjct: 426 ICERMPSPNGESAVDCSQLSKMPTVSFTIGGKVFDLAPEEYVLKIGEGPVAQCISGFTAL 485

Query: 482 DIPPPRGPLWILGDVFMGPYHTVFDFGKARVGFAEA 516
           DIPPPRGPLWILGDVFMG YHTVFDFG  +VGFAEA
Sbjct: 486 DIPPPRGPLWILGDVFMGKYHTVFDFGNEQVGFAEA 512

BLAST of CsGy6G032920 vs. ExPASy Swiss-Prot
Match: O65390 (Aspartic proteinase A1 OS=Arabidopsis thaliana OX=3702 GN=APA1 PE=1 SV=1)

HSP 1 Score: 659.8 bits (1701), Expect = 2.5e-188
Identity = 323/509 (63.46%), Postives = 392/509 (77.01%), Query Frame = 0

Query: 9   LLVSLLLLILHYSSEATSASNEGFLRIGLKKIKYDQNSRFKALLESKKGEFLGSSVGKHN 68
           L+VS LL        A +  N+G  R+GLKK+K D  +R  A +ESK+ + L +      
Sbjct: 12  LIVSFLLCF-----SAFAERNDGTFRVGLKKLKLDSKNRLAARVESKQEKPLRA------ 71

Query: 69  QWGNNLEESKNADIVPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSAKCIFSL 128
                L +S +AD+V LKNYLDAQYYGEI IGTPPQKFTV+FDTGSSNLWVPS+KC FSL
Sbjct: 72  ---YRLGDSGDADVVVLKNYLDAQYYGEIAIGTPPQKFTVVFDTGSSNLWVPSSKCYFSL 131

Query: 129 ACFFHAKYQSGRSSTYKRNGTSAAIQYGSGAISGFFSYDNVQVGDVIVRNQELIEATSMS 188
           AC  H KY+S RSSTY++NG +AAI YG+GAI+GFFS D V VGD++V++QE IEAT   
Sbjct: 132 ACLLHPKYKSSRSSTYEKNGKAAAIHYGTGAIAGFFSNDAVTVGDLVVKDQEFIEATKEP 191

Query: 189 TMTFMAAKFDGILGLGFQEIATGGAVPVWYNMVKQKLVKEQVFSFWLNRNAEEKEGGELV 248
            +TF+ AKFDGILGLGFQEI+ G A PVWYNM+KQ L+KE VFSFWLNRNA+E+EGGELV
Sbjct: 192 GITFVVAKFDGILGLGFQEISVGKAAPVWYNMLKQGLIKEPVFSFWLNRNADEEEGGELV 251

Query: 249 FGGVDPKHFKGQHTYVPVTDKGYWQFDIGDILIGGETTKYCAGGCSAIADSGTSLLAGPS 308
           FGGVDP HFKG+HTYVPVT KGYWQFD+GD+LIGG  T +C  GCSAIADSGTSLLAGP+
Sbjct: 252 FGGVDPNHFKGKHTYVPVTQKGYWQFDMGDVLIGGAPTGFCESGCSAIADSGTSLLAGPT 311

Query: 309 NIVVSINRAIGAAAVAHPECKAIVSQYGRAIMDLLLAKAQPEKICSKIGVCTFDETHDVS 368
            I+  IN AIGAA V   +CK +V QYG+ I+DLLL++ QP+KICS+IG+CTFD T  VS
Sbjct: 312 TIITMINHAIGAAGVVSQQCKTVVDQYGQTILDLLLSETQPKKICSQIGLCTFDGTRGVS 371

Query: 369 LKIENVVSDKDGRSSGGFSEAMCSACEMAVLWIQDELKQNKTQEDIIENVNELCDR-GLN 428
           + IE+VV  ++ + S G  +A CSACEMAV+WIQ +L+QN TQE I+  VNELC+R    
Sbjct: 372 MGIESVVDKENAKLSNGVGDAACSACEMAVVWIQSQLRQNMTQERILNYVNELCERLPSP 431

Query: 429 QDETLVDCGRISQMPNVSFTIGDRLFELTSKDYILKVGEGSAAQCISGFIPFDIPPPRGP 488
             E+ VDC ++S MP VS TIG ++F+L  ++Y+LKVGEG  AQCISGFI  D+ PPRGP
Sbjct: 432 MGESAVDCAQLSTMPTVSLTIGGKVFDLAPEEYVLKVGEGPVAQCISGFIALDVAPPRGP 491

Query: 489 LWILGDVFMGPYHTVFDFGKARVGFAEAA 517
           LWILGDVFMG YHTVFDFG  +VGFAEAA
Sbjct: 492 LWILGDVFMGKYHTVFDFGNEQVGFAEAA 506

BLAST of CsGy6G032920 vs. ExPASy Swiss-Prot
Match: Q42456 (Aspartic proteinase oryzasin-1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0567100 PE=2 SV=2)

HSP 1 Score: 649.4 bits (1674), Expect = 3.3e-185
Identity = 317/508 (62.40%), Postives = 395/508 (77.76%), Query Frame = 0

Query: 10  LVSLLLLILHYSSEATSASNEGFLRIGLKKIKYDQNSRFKALLESKKGEFLGSSVGKHNQ 69
           LV L  ++L     A++A  EG +RI LKK   D+NSR  A L  ++G       G ++ 
Sbjct: 8   LVLLAAVLLQALLPASAA--EGLVRIALKKRPIDENSRVAARLSGEEGARRLGLRGANSL 67

Query: 70  WGNNLEESKNADIVPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSAKCIFSLA 129
            G   E     DIV LKNY++AQY+GEIG+GTPPQKFTVIFDTGSSNLWVPSAKC FS+A
Sbjct: 68  GGGGGE----GDIVALKNYMNAQYFGEIGVGTPPQKFTVIFDTGSSNLWVPSAKCYFSIA 127

Query: 130 CFFHAKYQSGRSSTYKRNGTSAAIQYGSGAISGFFSYDNVQVGDVIVRNQELIEATSMST 189
           CFFH++Y+SG+SSTY++NG  AAIQYG+G+I+GFFS D+V VGD++V++QE IEAT    
Sbjct: 128 CFFHSRYKSGQSSTYQKNGKPAAIQYGTGSIAGFFSEDSVTVGDLVVKDQEFIEATKEPG 187

Query: 190 MTFMAAKFDGILGLGFQEIATGGAVPVWYNMVKQKLVKEQVFSFWLNRNAEEKEGGELVF 249
           +TFM AKFDGILGLGFQEI+ G AVPVWY MV+Q LV E VFSFW NR+++E EGGE+VF
Sbjct: 188 LTFMVAKFDGILGLGFQEISVGDAVPVWYKMVEQGLVSEPVFSFWFNRHSDEGEGGEIVF 247

Query: 250 GGVDPKHFKGQHTYVPVTDKGYWQFDIGDILIGGETTKYCAGGCSAIADSGTSLLAGPSN 309
           GG+DP H+KG HTYVPV+ KGYWQF++GD+LIGG+TT +CA GCSAIADSGTSLLAGP+ 
Sbjct: 248 GGMDPSHYKGNHTYVPVSQKGYWQFEMGDVLIGGKTTGFCASGCSAIADSGTSLLAGPTA 307

Query: 310 IVVSINRAIGAAAVAHPECKAIVSQYGRAIMDLLLAKAQPEKICSKIGVCTFDETHDVSL 369
           I+  IN  IGA  V   ECK +VSQYG+ I+DLLLA+ QP KICS++G+CTFD  H VS 
Sbjct: 308 IITEINEKIGATGVVSQECKTVVSQYGQQILDLLLAETQPSKICSQVGLCTFDGKHGVSA 367

Query: 370 KIENVVSDKDGRSSGGFSEAMCSACEMAVLWIQDELKQNKTQEDIIENVNELCDR-GLNQ 429
            I++VV D+ G S+G  S  MC+ACEMAV+W+Q++L QNKTQ+ I+  +N+LCD+     
Sbjct: 368 GIKSVVDDEAGESNGLQSGPMCNACEMAVVWMQNQLAQNKTQDLILNYINQLCDKLPSPM 427

Query: 430 DETLVDCGRISQMPNVSFTIGDRLFELTSKDYILKVGEGSAAQCISGFIPFDIPPPRGPL 489
            E+ VDCG ++ MP +SFTIG + F L  ++YILKVGEG+AAQCISGF   DIPPPRGPL
Sbjct: 428 GESSVDCGSLASMPEISFTIGGKKFALKPEEYILKVGEGAAAQCISGFTAMDIPPPRGPL 487

Query: 490 WILGDVFMGPYHTVFDFGKARVGFAEAA 517
           WILGDVFMG YHTVFD+GK RVGFA++A
Sbjct: 488 WILGDVFMGAYHTVFDYGKMRVGFAKSA 509

BLAST of CsGy6G032920 vs. ExPASy Swiss-Prot
Match: P42210 (Phytepsin OS=Hordeum vulgare OX=4513 PE=1 SV=1)

HSP 1 Score: 645.2 bits (1663), Expect = 6.3e-184
Identity = 314/511 (61.45%), Postives = 399/511 (78.08%), Query Frame = 0

Query: 9   LLVSLLLL--ILHYSSEATSASNEGFLRIGLKKIKYDQNSRFKALLESKKGEFLGSSVGK 68
           LL ++LLL  +L  +SEA     EG +RI LKK   D+NSR    L   + + L S    
Sbjct: 10  LLAAVLLLQTVLPAASEA-----EGLVRIALKKRPIDRNSRVATGLSGGEEQPLLSG--- 69

Query: 69  HNQWGNNLEESKNADIVPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSAKCIF 128
                N L   +  DIV LKNY++AQY+GEIG+GTPPQKFTVIFDTGSSNLWVPSAKC F
Sbjct: 70  ----ANPLRSEEEGDIVALKNYMNAQYFGEIGVGTPPQKFTVIFDTGSSNLWVPSAKCYF 129

Query: 129 SLACFFHAKYQSGRSSTYKRNGTSAAIQYGSGAISGFFSYDNVQVGDVIVRNQELIEATS 188
           S+AC+ H++Y++G SSTYK+NG  AAIQYG+G+I+G+FS D+V VGD++V++QE IEAT 
Sbjct: 130 SIACYLHSRYKAGASSTYKKNGKPAAIQYGTGSIAGYFSEDSVTVGDLVVKDQEFIEATK 189

Query: 189 MSTMTFMAAKFDGILGLGFQEIATGGAVPVWYNMVKQKLVKEQVFSFWLNRNAEEKEGGE 248
              +TF+ AKFDGILGLGF+EI+ G AVPVWY M++Q LV + VFSFWLNR+ +E EGGE
Sbjct: 190 EPGITFLVAKFDGILGLGFKEISVGKAVPVWYKMIEQGLVSDPVFSFWLNRHVDEGEGGE 249

Query: 249 LVFGGVDPKHFKGQHTYVPVTDKGYWQFDIGDILIGGETTKYCAGGCSAIADSGTSLLAG 308
           ++FGG+DPKH+ G+HTYVPVT KGYWQFD+GD+L+GG++T +CAGGC+AIADSGTSLLAG
Sbjct: 250 IIFGGMDPKHYVGEHTYVPVTQKGYWQFDMGDVLVGGKSTGFCAGGCAAIADSGTSLLAG 309

Query: 309 PSNIVVSINRAIGAAAVAHPECKAIVSQYGRAIMDLLLAKAQPEKICSKIGVCTFDETHD 368
           P+ I+  IN  IGAA V   ECK IVSQYG+ I+DLLLA+ QP+KICS++G+CTFD T  
Sbjct: 310 PTAIITEINEKIGAAGVVSQECKTIVSQYGQQILDLLLAETQPKKICSQVGLCTFDGTRG 369

Query: 369 VSLKIENVVSDKDGRSSGGFSEAMCSACEMAVLWIQDELKQNKTQEDIIENVNELCDR-G 428
           VS  I +VV D+  +S+G  ++ MCSACEMAV+W+Q++L QNKTQ+ I++ VN+LC+R  
Sbjct: 370 VSAGIRSVVDDEPVKSNGLRADPMCSACEMAVVWMQNQLAQNKTQDLILDYVNQLCNRLP 429

Query: 429 LNQDETLVDCGRISQMPNVSFTIGDRLFELTSKDYILKVGEGSAAQCISGFIPFDIPPPR 488
               E+ VDCG +  MP++ FTIG + F L  ++YILKVGEG+AAQCISGF   DIPPPR
Sbjct: 430 SPMGESAVDCGSLGSMPDIEFTIGGKKFALKPEEYILKVGEGAAAQCISGFTAMDIPPPR 489

Query: 489 GPLWILGDVFMGPYHTVFDFGKARVGFAEAA 517
           GPLWILGDVFMGPYHTVFD+GK R+GFA+AA
Sbjct: 490 GPLWILGDVFMGPYHTVFDYGKLRIGFAKAA 508

BLAST of CsGy6G032920 vs. NCBI nr
Match: XP_004134774.1 (aspartic proteinase [Cucumis sativus] >XP_011658133.1 aspartic proteinase [Cucumis sativus] >XP_031742358.1 aspartic proteinase [Cucumis sativus] >KGN49091.1 hypothetical protein Csa_003243 [Cucumis sativus])

HSP 1 Score: 1041 bits (2693), Expect = 0.0
Identity = 516/516 (100.00%), Postives = 516/516 (100.00%), Query Frame = 0

Query: 1   MRKISFSHLLVSLLLLILHYSSEATSASNEGFLRIGLKKIKYDQNSRFKALLESKKGEFL 60
           MRKISFSHLLVSLLLLILHYSSEATSASNEGFLRIGLKKIKYDQNSRFKALLESKKGEFL
Sbjct: 1   MRKISFSHLLVSLLLLILHYSSEATSASNEGFLRIGLKKIKYDQNSRFKALLESKKGEFL 60

Query: 61  GSSVGKHNQWGNNLEESKNADIVPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVP 120
           GSSVGKHNQWGNNLEESKNADIVPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVP
Sbjct: 61  GSSVGKHNQWGNNLEESKNADIVPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVP 120

Query: 121 SAKCIFSLACFFHAKYQSGRSSTYKRNGTSAAIQYGSGAISGFFSYDNVQVGDVIVRNQE 180
           SAKCIFSLACFFHAKYQSGRSSTYKRNGTSAAIQYGSGAISGFFSYDNVQVGDVIVRNQE
Sbjct: 121 SAKCIFSLACFFHAKYQSGRSSTYKRNGTSAAIQYGSGAISGFFSYDNVQVGDVIVRNQE 180

Query: 181 LIEATSMSTMTFMAAKFDGILGLGFQEIATGGAVPVWYNMVKQKLVKEQVFSFWLNRNAE 240
           LIEATSMSTMTFMAAKFDGILGLGFQEIATGGAVPVWYNMVKQKLVKEQVFSFWLNRNAE
Sbjct: 181 LIEATSMSTMTFMAAKFDGILGLGFQEIATGGAVPVWYNMVKQKLVKEQVFSFWLNRNAE 240

Query: 241 EKEGGELVFGGVDPKHFKGQHTYVPVTDKGYWQFDIGDILIGGETTKYCAGGCSAIADSG 300
           EKEGGELVFGGVDPKHFKGQHTYVPVTDKGYWQFDIGDILIGGETTKYCAGGCSAIADSG
Sbjct: 241 EKEGGELVFGGVDPKHFKGQHTYVPVTDKGYWQFDIGDILIGGETTKYCAGGCSAIADSG 300

Query: 301 TSLLAGPSNIVVSINRAIGAAAVAHPECKAIVSQYGRAIMDLLLAKAQPEKICSKIGVCT 360
           TSLLAGPSNIVVSINRAIGAAAVAHPECKAIVSQYGRAIMDLLLAKAQPEKICSKIGVCT
Sbjct: 301 TSLLAGPSNIVVSINRAIGAAAVAHPECKAIVSQYGRAIMDLLLAKAQPEKICSKIGVCT 360

Query: 361 FDETHDVSLKIENVVSDKDGRSSGGFSEAMCSACEMAVLWIQDELKQNKTQEDIIENVNE 420
           FDETHDVSLKIENVVSDKDGRSSGGFSEAMCSACEMAVLWIQDELKQNKTQEDIIENVNE
Sbjct: 361 FDETHDVSLKIENVVSDKDGRSSGGFSEAMCSACEMAVLWIQDELKQNKTQEDIIENVNE 420

Query: 421 LCDRGLNQDETLVDCGRISQMPNVSFTIGDRLFELTSKDYILKVGEGSAAQCISGFIPFD 480
           LCDRGLNQDETLVDCGRISQMPNVSFTIGDRLFELTSKDYILKVGEGSAAQCISGFIPFD
Sbjct: 421 LCDRGLNQDETLVDCGRISQMPNVSFTIGDRLFELTSKDYILKVGEGSAAQCISGFIPFD 480

Query: 481 IPPPRGPLWILGDVFMGPYHTVFDFGKARVGFAEAA 516
           IPPPRGPLWILGDVFMGPYHTVFDFGKARVGFAEAA
Sbjct: 481 IPPPRGPLWILGDVFMGPYHTVFDFGKARVGFAEAA 516

BLAST of CsGy6G032920 vs. NCBI nr
Match: XP_008440021.1 (PREDICTED: aspartic proteinase-like isoform X1 [Cucumis melo])

HSP 1 Score: 978 bits (2528), Expect = 0.0
Identity = 480/516 (93.02%), Postives = 504/516 (97.67%), Query Frame = 0

Query: 1   MRKISFSHLLVSLLLLILHYSSEATSASNEGFLRIGLKKIKYDQNSRFKALLESKKGEFL 60
           MRKISFSHLLVSLLLLILHYSSEATSASNEGFLRIGLKKI+ DQNSRFKALLESKKGEFL
Sbjct: 33  MRKISFSHLLVSLLLLILHYSSEATSASNEGFLRIGLKKIRSDQNSRFKALLESKKGEFL 92

Query: 61  GSSVGKHNQWGNNLEESKNADIVPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVP 120
           G SVGKHNQWGNNL ESKNAD VPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVP
Sbjct: 93  GPSVGKHNQWGNNLGESKNADNVPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVP 152

Query: 121 SAKCIFSLACFFHAKYQSGRSSTYKRNGTSAAIQYGSGAISGFFSYDNVQVGDVIVRNQE 180
           S+KC+FSLACFFHA+YQSGRSSTYK+NGTSAAIQYGSGAI+GFFS DNV+VGDV+VRNQ+
Sbjct: 153 SSKCVFSLACFFHARYQSGRSSTYKKNGTSAAIQYGSGAIAGFFSSDNVRVGDVVVRNQD 212

Query: 181 LIEATSMSTMTFMAAKFDGILGLGFQEIATGGAVPVWYNMVKQKLVKEQVFSFWLNRNAE 240
           LIEATSMS+MTFMAAKFDGILGLGFQEI+TGGAVPVWYNMVKQKLVKEQVFSFWLNRNA+
Sbjct: 213 LIEATSMSSMTFMAAKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNAK 272

Query: 241 EKEGGELVFGGVDPKHFKGQHTYVPVTDKGYWQFDIGDILIGGETTKYCAGGCSAIADSG 300
           E+EGGELVFGGVDPKHFKGQHTYVPVT+KGYWQFDIGDILIGGETTKYCA GCSAIADSG
Sbjct: 273 EEEGGELVFGGVDPKHFKGQHTYVPVTNKGYWQFDIGDILIGGETTKYCASGCSAIADSG 332

Query: 301 TSLLAGPSNIVVSINRAIGAAAVAHPECKAIVSQYGRAIMDLLLAKAQPEKICSKIGVCT 360
           TSLLAGPS+IVV INRAIGAAAVAHPECKAIVSQ+GRAIMDLLLAKAQPEKICS IGVCT
Sbjct: 333 TSLLAGPSDIVVLINRAIGAAAVAHPECKAIVSQHGRAIMDLLLAKAQPEKICSIIGVCT 392

Query: 361 FDETHDVSLKIENVVSDKDGRSSGGFSEAMCSACEMAVLWIQDELKQNKTQEDIIENVNE 420
           FD+T DVSLKIENVVSDKDGRSSGGFSEAMCSACEMAV WIQDEL+QNKTQEDII+NVNE
Sbjct: 393 FDQTRDVSLKIENVVSDKDGRSSGGFSEAMCSACEMAVSWIQDELRQNKTQEDIIDNVNE 452

Query: 421 LCDRGLNQDETLVDCGRISQMPNVSFTIGDRLFELTSKDYILKVGEGSAAQCISGFIPFD 480
           LCDRG NQ+ETLVDCGRISQMP+VSFTIGDR+FEL+SKDYILKVGEGSAAQCISGFIP D
Sbjct: 453 LCDRGSNQEETLVDCGRISQMPSVSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLD 512

Query: 481 IPPPRGPLWILGDVFMGPYHTVFDFGKARVGFAEAA 516
           IPPPRGPLWILGDVFMGPYHTVFDFGKARVGFA+AA
Sbjct: 513 IPPPRGPLWILGDVFMGPYHTVFDFGKARVGFADAA 548

BLAST of CsGy6G032920 vs. NCBI nr
Match: XP_008440022.1 (PREDICTED: aspartic proteinase-like isoform X2 [Cucumis melo] >XP_008440023.1 PREDICTED: aspartic proteinase-like isoform X2 [Cucumis melo] >XP_008440024.1 PREDICTED: aspartic proteinase-like isoform X2 [Cucumis melo] >XP_008440026.1 PREDICTED: aspartic proteinase-like isoform X2 [Cucumis melo] >XP_016899345.1 PREDICTED: aspartic proteinase-like isoform X2 [Cucumis melo])

HSP 1 Score: 978 bits (2528), Expect = 0.0
Identity = 480/516 (93.02%), Postives = 504/516 (97.67%), Query Frame = 0

Query: 1   MRKISFSHLLVSLLLLILHYSSEATSASNEGFLRIGLKKIKYDQNSRFKALLESKKGEFL 60
           MRKISFSHLLVSLLLLILHYSSEATSASNEGFLRIGLKKI+ DQNSRFKALLESKKGEFL
Sbjct: 1   MRKISFSHLLVSLLLLILHYSSEATSASNEGFLRIGLKKIRSDQNSRFKALLESKKGEFL 60

Query: 61  GSSVGKHNQWGNNLEESKNADIVPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVP 120
           G SVGKHNQWGNNL ESKNAD VPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVP
Sbjct: 61  GPSVGKHNQWGNNLGESKNADNVPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVP 120

Query: 121 SAKCIFSLACFFHAKYQSGRSSTYKRNGTSAAIQYGSGAISGFFSYDNVQVGDVIVRNQE 180
           S+KC+FSLACFFHA+YQSGRSSTYK+NGTSAAIQYGSGAI+GFFS DNV+VGDV+VRNQ+
Sbjct: 121 SSKCVFSLACFFHARYQSGRSSTYKKNGTSAAIQYGSGAIAGFFSSDNVRVGDVVVRNQD 180

Query: 181 LIEATSMSTMTFMAAKFDGILGLGFQEIATGGAVPVWYNMVKQKLVKEQVFSFWLNRNAE 240
           LIEATSMS+MTFMAAKFDGILGLGFQEI+TGGAVPVWYNMVKQKLVKEQVFSFWLNRNA+
Sbjct: 181 LIEATSMSSMTFMAAKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNAK 240

Query: 241 EKEGGELVFGGVDPKHFKGQHTYVPVTDKGYWQFDIGDILIGGETTKYCAGGCSAIADSG 300
           E+EGGELVFGGVDPKHFKGQHTYVPVT+KGYWQFDIGDILIGGETTKYCA GCSAIADSG
Sbjct: 241 EEEGGELVFGGVDPKHFKGQHTYVPVTNKGYWQFDIGDILIGGETTKYCASGCSAIADSG 300

Query: 301 TSLLAGPSNIVVSINRAIGAAAVAHPECKAIVSQYGRAIMDLLLAKAQPEKICSKIGVCT 360
           TSLLAGPS+IVV INRAIGAAAVAHPECKAIVSQ+GRAIMDLLLAKAQPEKICS IGVCT
Sbjct: 301 TSLLAGPSDIVVLINRAIGAAAVAHPECKAIVSQHGRAIMDLLLAKAQPEKICSIIGVCT 360

Query: 361 FDETHDVSLKIENVVSDKDGRSSGGFSEAMCSACEMAVLWIQDELKQNKTQEDIIENVNE 420
           FD+T DVSLKIENVVSDKDGRSSGGFSEAMCSACEMAV WIQDEL+QNKTQEDII+NVNE
Sbjct: 361 FDQTRDVSLKIENVVSDKDGRSSGGFSEAMCSACEMAVSWIQDELRQNKTQEDIIDNVNE 420

Query: 421 LCDRGLNQDETLVDCGRISQMPNVSFTIGDRLFELTSKDYILKVGEGSAAQCISGFIPFD 480
           LCDRG NQ+ETLVDCGRISQMP+VSFTIGDR+FEL+SKDYILKVGEGSAAQCISGFIP D
Sbjct: 421 LCDRGSNQEETLVDCGRISQMPSVSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLD 480

Query: 481 IPPPRGPLWILGDVFMGPYHTVFDFGKARVGFAEAA 516
           IPPPRGPLWILGDVFMGPYHTVFDFGKARVGFA+AA
Sbjct: 481 IPPPRGPLWILGDVFMGPYHTVFDFGKARVGFADAA 516

BLAST of CsGy6G032920 vs. NCBI nr
Match: TYK13029.1 (aspartic proteinase-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 978 bits (2527), Expect = 0.0
Identity = 480/516 (93.02%), Postives = 505/516 (97.87%), Query Frame = 0

Query: 1   MRKISFSHLLVSLLLLILHYSSEATSASNEGFLRIGLKKIKYDQNSRFKALLESKKGEFL 60
           MRKISFSHLLVSLLLLILHYSSEATSASNEGFLRIGLKKI+ DQNSRFKALLESKKGEFL
Sbjct: 1   MRKISFSHLLVSLLLLILHYSSEATSASNEGFLRIGLKKIRSDQNSRFKALLESKKGEFL 60

Query: 61  GSSVGKHNQWGNNLEESKNADIVPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVP 120
           GSSVGK+NQWGNNL ESKNAD VPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVP
Sbjct: 61  GSSVGKYNQWGNNLGESKNADNVPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVP 120

Query: 121 SAKCIFSLACFFHAKYQSGRSSTYKRNGTSAAIQYGSGAISGFFSYDNVQVGDVIVRNQE 180
           S+KC+FSLACFFHA+YQSGRSSTYK+NGTSAAIQYGSGAI+GFFS DNV+VGDV+VRNQ+
Sbjct: 121 SSKCVFSLACFFHARYQSGRSSTYKKNGTSAAIQYGSGAIAGFFSSDNVRVGDVVVRNQD 180

Query: 181 LIEATSMSTMTFMAAKFDGILGLGFQEIATGGAVPVWYNMVKQKLVKEQVFSFWLNRNAE 240
           LIEATSMS+MTFMAAKFDGILGLGFQEI+TGGAVPVWYNMVKQKLVKEQVFSFWLNRNA+
Sbjct: 181 LIEATSMSSMTFMAAKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNAK 240

Query: 241 EKEGGELVFGGVDPKHFKGQHTYVPVTDKGYWQFDIGDILIGGETTKYCAGGCSAIADSG 300
           E+EGGELVFGGVDPKHFKGQHTYVPVT+KGYWQFDIGDILIGGETTKYCA GCSAIADSG
Sbjct: 241 EEEGGELVFGGVDPKHFKGQHTYVPVTNKGYWQFDIGDILIGGETTKYCASGCSAIADSG 300

Query: 301 TSLLAGPSNIVVSINRAIGAAAVAHPECKAIVSQYGRAIMDLLLAKAQPEKICSKIGVCT 360
           TSLLAGPS+IVV INRAIGAAAVAHPECKAIVSQ+GRAIMDLLLAKAQPEKICS IGVCT
Sbjct: 301 TSLLAGPSDIVVLINRAIGAAAVAHPECKAIVSQHGRAIMDLLLAKAQPEKICSIIGVCT 360

Query: 361 FDETHDVSLKIENVVSDKDGRSSGGFSEAMCSACEMAVLWIQDELKQNKTQEDIIENVNE 420
           FD+T DVSLKIENVVSDKDGRSSGGFSEAMCSACEMAV WIQDEL+QNKTQEDII+NVNE
Sbjct: 361 FDQTRDVSLKIENVVSDKDGRSSGGFSEAMCSACEMAVSWIQDELRQNKTQEDIIDNVNE 420

Query: 421 LCDRGLNQDETLVDCGRISQMPNVSFTIGDRLFELTSKDYILKVGEGSAAQCISGFIPFD 480
           LCDRG NQ+ETLVDCGRISQMP+VSFTIGDR+FEL+SKDYILKVGEGSAAQCISGFIP D
Sbjct: 421 LCDRGSNQEETLVDCGRISQMPSVSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLD 480

Query: 481 IPPPRGPLWILGDVFMGPYHTVFDFGKARVGFAEAA 516
           IPPPRGPLWILGDVFMGPYHTVFDFGKARVGFA+AA
Sbjct: 481 IPPPRGPLWILGDVFMGPYHTVFDFGKARVGFADAA 516

BLAST of CsGy6G032920 vs. NCBI nr
Match: XP_038880988.1 (aspartic proteinase-like isoform X2 [Benincasa hispida])

HSP 1 Score: 919 bits (2374), Expect = 0.0
Identity = 451/516 (87.40%), Postives = 481/516 (93.22%), Query Frame = 0

Query: 1   MRKISFSHLLVSLLLLILHYSSEATSASNEGFLRIGLKKIKYDQNSRFKALLESKKGEFL 60
           M + SF  LLVSLLLLIL+YSSEA+SASNEGFLRIGLKKIK DQN RFKALLESKKGEFL
Sbjct: 1   MMRNSFKPLLVSLLLLILYYSSEASSASNEGFLRIGLKKIKADQNGRFKALLESKKGEFL 60

Query: 61  GSSVGKHNQWGNNLEESKNADIVPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVP 120
           GSSVGKHNQWGNN+ ES+N DIV LKNYLDAQYYGEIGIGTPPQKFTV+FDTGSSNLWVP
Sbjct: 61  GSSVGKHNQWGNNIGESRNTDIVALKNYLDAQYYGEIGIGTPPQKFTVVFDTGSSNLWVP 120

Query: 121 SAKCIFSLACFFHAKYQSGRSSTYKRNGTSAAIQYGSGAISGFFSYDNVQVGDVIVRNQE 180
           S+KCIFSLACFFHA+YQSGRSSTY+RNGTSA+IQYGSGAI+GFFSYDNV+VGDV+V +QE
Sbjct: 121 SSKCIFSLACFFHARYQSGRSSTYRRNGTSASIQYGSGAIAGFFSYDNVRVGDVVVHDQE 180

Query: 181 LIEATSMSTMTFMAAKFDGILGLGFQEIATGGAVPVWYNMVKQKLVKEQVFSFWLNRNAE 240
           LIEATSMS+MTFM AKFDGILGLGFQEI+TGGAVPVWYNMVKQKLVKEQVFSFWLNRNA 
Sbjct: 181 LIEATSMSSMTFMTAKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNAG 240

Query: 241 EKEGGELVFGGVDPKHFKGQHTYVPVTDKGYWQFDIGDILIGGETTKYCAGGCSAIADSG 300
           E EGGELVFGGVDPKHFKGQHTYVPVT KGYWQFDIGDILIGGETT+YCA GCSAIADSG
Sbjct: 241 EAEGGELVFGGVDPKHFKGQHTYVPVTTKGYWQFDIGDILIGGETTEYCAHGCSAIADSG 300

Query: 301 TSLLAGPSNIVVSINRAIGAAAVAHPECKAIVSQYGRAIMDLLLAKAQPEKICSKIGVCT 360
           TSLLAGPS IV  INRAIGAA VA PECKAIVSQ+G+AIMDLLL  AQPEKICSKIGVCT
Sbjct: 301 TSLLAGPSTIVALINRAIGAAEVASPECKAIVSQHGQAIMDLLLVNAQPEKICSKIGVCT 360

Query: 361 FDETHDVSLKIENVVSDKDGRSSGGFSEAMCSACEMAVLWIQDELKQNKTQEDIIENVNE 420
           FD+T  V LKIE +V+DKDG+SSGGFS+AMCSACEMAV W+QDELKQNKTQE II+ VNE
Sbjct: 361 FDDTRGVRLKIETLVNDKDGKSSGGFSDAMCSACEMAVSWMQDELKQNKTQEHIIDYVNE 420

Query: 421 LCDRGLNQDETLVDCGRISQMPNVSFTIGDRLFELTSKDYILKVGEGSAAQCISGFIPFD 480
           LCDRGLNQ  TLVDCGRIS+MP VSFTIGDR+FEL+SKDYILKVGEGSAAQCISGFIP D
Sbjct: 421 LCDRGLNQGATLVDCGRISRMPTVSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLD 480

Query: 481 IPPPRGPLWILGDVFMGPYHTVFDFGKARVGFAEAA 516
           IPPPRGPLWILGDVFMG YHTVFDFGK RVGFA+AA
Sbjct: 481 IPPPRGPLWILGDVFMGRYHTVFDFGKVRVGFADAA 516

BLAST of CsGy6G032920 vs. ExPASy TrEMBL
Match: A0A0A0KMZ9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G513550 PE=3 SV=1)

HSP 1 Score: 1041 bits (2693), Expect = 0.0
Identity = 516/516 (100.00%), Postives = 516/516 (100.00%), Query Frame = 0

Query: 1   MRKISFSHLLVSLLLLILHYSSEATSASNEGFLRIGLKKIKYDQNSRFKALLESKKGEFL 60
           MRKISFSHLLVSLLLLILHYSSEATSASNEGFLRIGLKKIKYDQNSRFKALLESKKGEFL
Sbjct: 1   MRKISFSHLLVSLLLLILHYSSEATSASNEGFLRIGLKKIKYDQNSRFKALLESKKGEFL 60

Query: 61  GSSVGKHNQWGNNLEESKNADIVPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVP 120
           GSSVGKHNQWGNNLEESKNADIVPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVP
Sbjct: 61  GSSVGKHNQWGNNLEESKNADIVPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVP 120

Query: 121 SAKCIFSLACFFHAKYQSGRSSTYKRNGTSAAIQYGSGAISGFFSYDNVQVGDVIVRNQE 180
           SAKCIFSLACFFHAKYQSGRSSTYKRNGTSAAIQYGSGAISGFFSYDNVQVGDVIVRNQE
Sbjct: 121 SAKCIFSLACFFHAKYQSGRSSTYKRNGTSAAIQYGSGAISGFFSYDNVQVGDVIVRNQE 180

Query: 181 LIEATSMSTMTFMAAKFDGILGLGFQEIATGGAVPVWYNMVKQKLVKEQVFSFWLNRNAE 240
           LIEATSMSTMTFMAAKFDGILGLGFQEIATGGAVPVWYNMVKQKLVKEQVFSFWLNRNAE
Sbjct: 181 LIEATSMSTMTFMAAKFDGILGLGFQEIATGGAVPVWYNMVKQKLVKEQVFSFWLNRNAE 240

Query: 241 EKEGGELVFGGVDPKHFKGQHTYVPVTDKGYWQFDIGDILIGGETTKYCAGGCSAIADSG 300
           EKEGGELVFGGVDPKHFKGQHTYVPVTDKGYWQFDIGDILIGGETTKYCAGGCSAIADSG
Sbjct: 241 EKEGGELVFGGVDPKHFKGQHTYVPVTDKGYWQFDIGDILIGGETTKYCAGGCSAIADSG 300

Query: 301 TSLLAGPSNIVVSINRAIGAAAVAHPECKAIVSQYGRAIMDLLLAKAQPEKICSKIGVCT 360
           TSLLAGPSNIVVSINRAIGAAAVAHPECKAIVSQYGRAIMDLLLAKAQPEKICSKIGVCT
Sbjct: 301 TSLLAGPSNIVVSINRAIGAAAVAHPECKAIVSQYGRAIMDLLLAKAQPEKICSKIGVCT 360

Query: 361 FDETHDVSLKIENVVSDKDGRSSGGFSEAMCSACEMAVLWIQDELKQNKTQEDIIENVNE 420
           FDETHDVSLKIENVVSDKDGRSSGGFSEAMCSACEMAVLWIQDELKQNKTQEDIIENVNE
Sbjct: 361 FDETHDVSLKIENVVSDKDGRSSGGFSEAMCSACEMAVLWIQDELKQNKTQEDIIENVNE 420

Query: 421 LCDRGLNQDETLVDCGRISQMPNVSFTIGDRLFELTSKDYILKVGEGSAAQCISGFIPFD 480
           LCDRGLNQDETLVDCGRISQMPNVSFTIGDRLFELTSKDYILKVGEGSAAQCISGFIPFD
Sbjct: 421 LCDRGLNQDETLVDCGRISQMPNVSFTIGDRLFELTSKDYILKVGEGSAAQCISGFIPFD 480

Query: 481 IPPPRGPLWILGDVFMGPYHTVFDFGKARVGFAEAA 516
           IPPPRGPLWILGDVFMGPYHTVFDFGKARVGFAEAA
Sbjct: 481 IPPPRGPLWILGDVFMGPYHTVFDFGKARVGFAEAA 516

BLAST of CsGy6G032920 vs. ExPASy TrEMBL
Match: A0A1S3B040 (aspartic proteinase-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103484625 PE=3 SV=1)

HSP 1 Score: 978 bits (2528), Expect = 0.0
Identity = 480/516 (93.02%), Postives = 504/516 (97.67%), Query Frame = 0

Query: 1   MRKISFSHLLVSLLLLILHYSSEATSASNEGFLRIGLKKIKYDQNSRFKALLESKKGEFL 60
           MRKISFSHLLVSLLLLILHYSSEATSASNEGFLRIGLKKI+ DQNSRFKALLESKKGEFL
Sbjct: 1   MRKISFSHLLVSLLLLILHYSSEATSASNEGFLRIGLKKIRSDQNSRFKALLESKKGEFL 60

Query: 61  GSSVGKHNQWGNNLEESKNADIVPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVP 120
           G SVGKHNQWGNNL ESKNAD VPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVP
Sbjct: 61  GPSVGKHNQWGNNLGESKNADNVPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVP 120

Query: 121 SAKCIFSLACFFHAKYQSGRSSTYKRNGTSAAIQYGSGAISGFFSYDNVQVGDVIVRNQE 180
           S+KC+FSLACFFHA+YQSGRSSTYK+NGTSAAIQYGSGAI+GFFS DNV+VGDV+VRNQ+
Sbjct: 121 SSKCVFSLACFFHARYQSGRSSTYKKNGTSAAIQYGSGAIAGFFSSDNVRVGDVVVRNQD 180

Query: 181 LIEATSMSTMTFMAAKFDGILGLGFQEIATGGAVPVWYNMVKQKLVKEQVFSFWLNRNAE 240
           LIEATSMS+MTFMAAKFDGILGLGFQEI+TGGAVPVWYNMVKQKLVKEQVFSFWLNRNA+
Sbjct: 181 LIEATSMSSMTFMAAKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNAK 240

Query: 241 EKEGGELVFGGVDPKHFKGQHTYVPVTDKGYWQFDIGDILIGGETTKYCAGGCSAIADSG 300
           E+EGGELVFGGVDPKHFKGQHTYVPVT+KGYWQFDIGDILIGGETTKYCA GCSAIADSG
Sbjct: 241 EEEGGELVFGGVDPKHFKGQHTYVPVTNKGYWQFDIGDILIGGETTKYCASGCSAIADSG 300

Query: 301 TSLLAGPSNIVVSINRAIGAAAVAHPECKAIVSQYGRAIMDLLLAKAQPEKICSKIGVCT 360
           TSLLAGPS+IVV INRAIGAAAVAHPECKAIVSQ+GRAIMDLLLAKAQPEKICS IGVCT
Sbjct: 301 TSLLAGPSDIVVLINRAIGAAAVAHPECKAIVSQHGRAIMDLLLAKAQPEKICSIIGVCT 360

Query: 361 FDETHDVSLKIENVVSDKDGRSSGGFSEAMCSACEMAVLWIQDELKQNKTQEDIIENVNE 420
           FD+T DVSLKIENVVSDKDGRSSGGFSEAMCSACEMAV WIQDEL+QNKTQEDII+NVNE
Sbjct: 361 FDQTRDVSLKIENVVSDKDGRSSGGFSEAMCSACEMAVSWIQDELRQNKTQEDIIDNVNE 420

Query: 421 LCDRGLNQDETLVDCGRISQMPNVSFTIGDRLFELTSKDYILKVGEGSAAQCISGFIPFD 480
           LCDRG NQ+ETLVDCGRISQMP+VSFTIGDR+FEL+SKDYILKVGEGSAAQCISGFIP D
Sbjct: 421 LCDRGSNQEETLVDCGRISQMPSVSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLD 480

Query: 481 IPPPRGPLWILGDVFMGPYHTVFDFGKARVGFAEAA 516
           IPPPRGPLWILGDVFMGPYHTVFDFGKARVGFA+AA
Sbjct: 481 IPPPRGPLWILGDVFMGPYHTVFDFGKARVGFADAA 516

BLAST of CsGy6G032920 vs. ExPASy TrEMBL
Match: A0A1S3B058 (aspartic proteinase-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103484625 PE=3 SV=1)

HSP 1 Score: 978 bits (2528), Expect = 0.0
Identity = 480/516 (93.02%), Postives = 504/516 (97.67%), Query Frame = 0

Query: 1   MRKISFSHLLVSLLLLILHYSSEATSASNEGFLRIGLKKIKYDQNSRFKALLESKKGEFL 60
           MRKISFSHLLVSLLLLILHYSSEATSASNEGFLRIGLKKI+ DQNSRFKALLESKKGEFL
Sbjct: 33  MRKISFSHLLVSLLLLILHYSSEATSASNEGFLRIGLKKIRSDQNSRFKALLESKKGEFL 92

Query: 61  GSSVGKHNQWGNNLEESKNADIVPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVP 120
           G SVGKHNQWGNNL ESKNAD VPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVP
Sbjct: 93  GPSVGKHNQWGNNLGESKNADNVPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVP 152

Query: 121 SAKCIFSLACFFHAKYQSGRSSTYKRNGTSAAIQYGSGAISGFFSYDNVQVGDVIVRNQE 180
           S+KC+FSLACFFHA+YQSGRSSTYK+NGTSAAIQYGSGAI+GFFS DNV+VGDV+VRNQ+
Sbjct: 153 SSKCVFSLACFFHARYQSGRSSTYKKNGTSAAIQYGSGAIAGFFSSDNVRVGDVVVRNQD 212

Query: 181 LIEATSMSTMTFMAAKFDGILGLGFQEIATGGAVPVWYNMVKQKLVKEQVFSFWLNRNAE 240
           LIEATSMS+MTFMAAKFDGILGLGFQEI+TGGAVPVWYNMVKQKLVKEQVFSFWLNRNA+
Sbjct: 213 LIEATSMSSMTFMAAKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNAK 272

Query: 241 EKEGGELVFGGVDPKHFKGQHTYVPVTDKGYWQFDIGDILIGGETTKYCAGGCSAIADSG 300
           E+EGGELVFGGVDPKHFKGQHTYVPVT+KGYWQFDIGDILIGGETTKYCA GCSAIADSG
Sbjct: 273 EEEGGELVFGGVDPKHFKGQHTYVPVTNKGYWQFDIGDILIGGETTKYCASGCSAIADSG 332

Query: 301 TSLLAGPSNIVVSINRAIGAAAVAHPECKAIVSQYGRAIMDLLLAKAQPEKICSKIGVCT 360
           TSLLAGPS+IVV INRAIGAAAVAHPECKAIVSQ+GRAIMDLLLAKAQPEKICS IGVCT
Sbjct: 333 TSLLAGPSDIVVLINRAIGAAAVAHPECKAIVSQHGRAIMDLLLAKAQPEKICSIIGVCT 392

Query: 361 FDETHDVSLKIENVVSDKDGRSSGGFSEAMCSACEMAVLWIQDELKQNKTQEDIIENVNE 420
           FD+T DVSLKIENVVSDKDGRSSGGFSEAMCSACEMAV WIQDEL+QNKTQEDII+NVNE
Sbjct: 393 FDQTRDVSLKIENVVSDKDGRSSGGFSEAMCSACEMAVSWIQDELRQNKTQEDIIDNVNE 452

Query: 421 LCDRGLNQDETLVDCGRISQMPNVSFTIGDRLFELTSKDYILKVGEGSAAQCISGFIPFD 480
           LCDRG NQ+ETLVDCGRISQMP+VSFTIGDR+FEL+SKDYILKVGEGSAAQCISGFIP D
Sbjct: 453 LCDRGSNQEETLVDCGRISQMPSVSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLD 512

Query: 481 IPPPRGPLWILGDVFMGPYHTVFDFGKARVGFAEAA 516
           IPPPRGPLWILGDVFMGPYHTVFDFGKARVGFA+AA
Sbjct: 513 IPPPRGPLWILGDVFMGPYHTVFDFGKARVGFADAA 548

BLAST of CsGy6G032920 vs. ExPASy TrEMBL
Match: A0A5D3CRY9 (Aspartic proteinase-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G006300 PE=3 SV=1)

HSP 1 Score: 978 bits (2527), Expect = 0.0
Identity = 480/516 (93.02%), Postives = 505/516 (97.87%), Query Frame = 0

Query: 1   MRKISFSHLLVSLLLLILHYSSEATSASNEGFLRIGLKKIKYDQNSRFKALLESKKGEFL 60
           MRKISFSHLLVSLLLLILHYSSEATSASNEGFLRIGLKKI+ DQNSRFKALLESKKGEFL
Sbjct: 1   MRKISFSHLLVSLLLLILHYSSEATSASNEGFLRIGLKKIRSDQNSRFKALLESKKGEFL 60

Query: 61  GSSVGKHNQWGNNLEESKNADIVPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVP 120
           GSSVGK+NQWGNNL ESKNAD VPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVP
Sbjct: 61  GSSVGKYNQWGNNLGESKNADNVPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVP 120

Query: 121 SAKCIFSLACFFHAKYQSGRSSTYKRNGTSAAIQYGSGAISGFFSYDNVQVGDVIVRNQE 180
           S+KC+FSLACFFHA+YQSGRSSTYK+NGTSAAIQYGSGAI+GFFS DNV+VGDV+VRNQ+
Sbjct: 121 SSKCVFSLACFFHARYQSGRSSTYKKNGTSAAIQYGSGAIAGFFSSDNVRVGDVVVRNQD 180

Query: 181 LIEATSMSTMTFMAAKFDGILGLGFQEIATGGAVPVWYNMVKQKLVKEQVFSFWLNRNAE 240
           LIEATSMS+MTFMAAKFDGILGLGFQEI+TGGAVPVWYNMVKQKLVKEQVFSFWLNRNA+
Sbjct: 181 LIEATSMSSMTFMAAKFDGILGLGFQEISTGGAVPVWYNMVKQKLVKEQVFSFWLNRNAK 240

Query: 241 EKEGGELVFGGVDPKHFKGQHTYVPVTDKGYWQFDIGDILIGGETTKYCAGGCSAIADSG 300
           E+EGGELVFGGVDPKHFKGQHTYVPVT+KGYWQFDIGDILIGGETTKYCA GCSAIADSG
Sbjct: 241 EEEGGELVFGGVDPKHFKGQHTYVPVTNKGYWQFDIGDILIGGETTKYCASGCSAIADSG 300

Query: 301 TSLLAGPSNIVVSINRAIGAAAVAHPECKAIVSQYGRAIMDLLLAKAQPEKICSKIGVCT 360
           TSLLAGPS+IVV INRAIGAAAVAHPECKAIVSQ+GRAIMDLLLAKAQPEKICS IGVCT
Sbjct: 301 TSLLAGPSDIVVLINRAIGAAAVAHPECKAIVSQHGRAIMDLLLAKAQPEKICSIIGVCT 360

Query: 361 FDETHDVSLKIENVVSDKDGRSSGGFSEAMCSACEMAVLWIQDELKQNKTQEDIIENVNE 420
           FD+T DVSLKIENVVSDKDGRSSGGFSEAMCSACEMAV WIQDEL+QNKTQEDII+NVNE
Sbjct: 361 FDQTRDVSLKIENVVSDKDGRSSGGFSEAMCSACEMAVSWIQDELRQNKTQEDIIDNVNE 420

Query: 421 LCDRGLNQDETLVDCGRISQMPNVSFTIGDRLFELTSKDYILKVGEGSAAQCISGFIPFD 480
           LCDRG NQ+ETLVDCGRISQMP+VSFTIGDR+FEL+SKDYILKVGEGSAAQCISGFIP D
Sbjct: 421 LCDRGSNQEETLVDCGRISQMPSVSFTIGDRVFELSSKDYILKVGEGSAAQCISGFIPLD 480

Query: 481 IPPPRGPLWILGDVFMGPYHTVFDFGKARVGFAEAA 516
           IPPPRGPLWILGDVFMGPYHTVFDFGKARVGFA+AA
Sbjct: 481 IPPPRGPLWILGDVFMGPYHTVFDFGKARVGFADAA 516

BLAST of CsGy6G032920 vs. ExPASy TrEMBL
Match: A0A6J1IKS1 (aspartic proteinase-like OS=Cucurbita maxima OX=3661 GN=LOC111477945 PE=3 SV=1)

HSP 1 Score: 859 bits (2219), Expect = 6.83e-312
Identity = 422/512 (82.42%), Postives = 465/512 (90.82%), Query Frame = 0

Query: 5   SFSHLLVSLLLLILHYSSEATSASNEGFLRIGLKKIKYDQNSRFKALLESKKGEFLGSSV 64
           S   LLVSLLLLIL YSS A+SASNEG +RIGLKKIK ++N   KAL+ESKK EFLGS  
Sbjct: 4   SLKPLLVSLLLLIL-YSSTASSASNEGLMRIGLKKIKVNKNILLKALVESKKVEFLGS-- 63

Query: 65  GKHNQWGNNLEESKNADIVPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSAKC 124
            KH+QWGN+L ESKN+DIV LKNY+DAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPS+KC
Sbjct: 64  -KHDQWGNDLGESKNSDIVALKNYMDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSSKC 123

Query: 125 IFSLACFFHAKYQSGRSSTYKRNGTSAAIQYGSGAISGFFSYDNVQVGDVIVRNQELIEA 184
           +FS+ACFFHA+YQSGRSSTY+RNGTSAAIQYGSGAISGFFSYDNVQVGDV+VRNQ+ IE 
Sbjct: 124 VFSIACFFHARYQSGRSSTYRRNGTSAAIQYGSGAISGFFSYDNVQVGDVVVRNQQFIET 183

Query: 185 TSMSTMTFMAAKFDGILGLGFQEIATGGAVPVWYNMVKQKLVKEQVFSFWLNRNAEEKEG 244
           TSMS+MTF+AAKFDGILGLGFQEI+TG AVPVWYNMVKQKLVKE VFSFWLNRNAEE+EG
Sbjct: 184 TSMSSMTFIAAKFDGILGLGFQEISTGDAVPVWYNMVKQKLVKEPVFSFWLNRNAEEEEG 243

Query: 245 GELVFGGVDPKHFKGQHTYVPVTDKGYWQFDIGDILIGGETTKYCAGGCSAIADSGTSLL 304
           GE+VFGGVDPKHFKGQHTYVPVT KGYWQF+IGDILIGG+ T+YCA GCSAIADSGTSLL
Sbjct: 244 GEIVFGGVDPKHFKGQHTYVPVTTKGYWQFNIGDILIGGKPTEYCARGCSAIADSGTSLL 303

Query: 305 AGPSNIVVSINRAIGAAAVAHPECKAIVSQYGRAIMDLLLAKAQPEKICSKIGVCTFDET 364
           AGPS IV  INRAIGAA +  PECK +VSQ+G++IMDLLLAK QPEKICSKIGVC FD +
Sbjct: 304 AGPSTIVTLINRAIGAAGIGRPECKEVVSQHGKSIMDLLLAKVQPEKICSKIGVCAFDGS 363

Query: 365 HDVSLKIENVVSDKDGRSSGGFSEAMCSACEMAVLWIQDELKQNKTQEDIIENVNELCDR 424
           H VS KIE+VV++KDG SSGGFS+AMCSACEMAV W+ DELKQNKTQE +I+ VN+LCDR
Sbjct: 364 HGVSKKIESVVNEKDGTSSGGFSDAMCSACEMAVSWMNDELKQNKTQEHVIDYVNKLCDR 423

Query: 425 GLNQDETLVDCGRISQMPNVSFTIGDRLFELTSKDYILKVGEGSAAQCISGFIPFDIPPP 484
            LN+ ETLVDCGRISQMP VSFTIGD++FEL ++DYILKVGEGSAAQCISGFIP DIPPP
Sbjct: 424 DLNEGETLVDCGRISQMPTVSFTIGDKVFELNAEDYILKVGEGSAAQCISGFIPLDIPPP 483

Query: 485 RGPLWILGDVFMGPYHTVFDFGKARVGFAEAA 516
           RGPLWILGDVFMG YHTVFDFGK RVGFAEAA
Sbjct: 484 RGPLWILGDVFMGRYHTVFDFGKLRVGFAEAA 511

BLAST of CsGy6G032920 vs. TAIR 10
Match: AT1G62290.1 (Saposin-like aspartyl protease family protein )

HSP 1 Score: 677.9 bits (1748), Expect = 6.2e-195
Identity = 328/516 (63.57%), Postives = 406/516 (78.68%), Query Frame = 0

Query: 2   RKISFSHLLVSLLLLILHYSSEATSASNEGFLRIGLKKIKYDQNSRFKALLESKKGEFLG 61
           R ++FS + VS LL    YS       N+G  R+GLKK+K D N+R      SK+ E L 
Sbjct: 6   RAVAFS-VFVSFLLFFTAYSKR-----NDGTFRVGLKKLKLDPNNRLATRFGSKQEEALR 65

Query: 62  SSVGKHNQWGNNL-EESKNADIVPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVP 121
           SS+  +N   NNL  +S +ADIVPLKNYLDAQYYGEI IGTPPQKFTVIFDTGSSNLWVP
Sbjct: 66  SSLRSYN---NNLGGDSGDADIVPLKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVP 125

Query: 122 SAKCIFSLACFFHAKYQSGRSSTYKRNGTSAAIQYGSGAISGFFSYDNVQVGDVIVRNQE 181
           S KC FSL+C+FHAKY+S RSSTYK++G  AAI YGSG+ISGFFSYD V VGD++V++QE
Sbjct: 126 SGKCFFSLSCYFHAKYKSSRSSTYKKSGKRAAIHYGSGSISGFFSYDAVTVGDLVVKDQE 185

Query: 182 LIEATSMSTMTFMAAKFDGILGLGFQEIATGGAVPVWYNMVKQKLVKEQVFSFWLNRNAE 241
            IE TS   +TF+ AKFDG+LGLGFQEIA G A PVWYNM+KQ L+K  VFSFWLNR+ +
Sbjct: 186 FIETTSEPGLTFLVAKFDGLLGLGFQEIAVGNATPVWYNMLKQGLIKRPVFSFWLNRDPK 245

Query: 242 EKEGGELVFGGVDPKHFKGQHTYVPVTDKGYWQFDIGDILIGGETTKYCAGGCSAIADSG 301
            +EGGE+VFGGVDPKHF+G+HT+VPVT +GYWQFD+G++LI GE+T YC  GCSAIADSG
Sbjct: 246 SEEGGEIVFGGVDPKHFRGEHTFVPVTQRGYWQFDMGEVLIAGESTGYCGSGCSAIADSG 305

Query: 302 TSLLAGPSNIVVSINRAIGAAAVAHPECKAIVSQYGRAIMDLLLAKAQPEKICSKIGVCT 361
           TSLLAGP+ +V  IN+AIGA+ V   +CK +V QYG+ I+DLLLA+ QP+KICS+IG+C 
Sbjct: 306 TSLLAGPTAVVAMINKAIGASGVVSQQCKTVVDQYGQTILDLLLAETQPKKICSQIGLCA 365

Query: 362 FDETHDVSLKIENVVSDKDGRSSGGFSEAMCSACEMAVLWIQDELKQNKTQEDIIENVNE 421
           +D TH VS+ IE+VV  ++ RSS G  +A C ACEMAV+WIQ +L+QN TQE I+  +NE
Sbjct: 366 YDGTHGVSMGIESVVDKENTRSSSGLRDAGCPACEMAVVWIQSQLRQNMTQERIVNYINE 425

Query: 422 LCDRGLNQD-ETLVDCGRISQMPNVSFTIGDRLFELTSKDYILKVGEGSAAQCISGFIPF 481
           +C+R  + + E+ VDC ++S+MP VSFTIG ++F+L  ++Y+LK+GEG  AQCISGF   
Sbjct: 426 ICERMPSPNGESAVDCSQLSKMPTVSFTIGGKVFDLAPEEYVLKIGEGPVAQCISGFTAL 485

Query: 482 DIPPPRGPLWILGDVFMGPYHTVFDFGKARVGFAEA 516
           DIPPPRGPLWILGDVFMG YHTVFDFG  +VGFAEA
Sbjct: 486 DIPPPRGPLWILGDVFMGKYHTVFDFGNEQVGFAEA 512

BLAST of CsGy6G032920 vs. TAIR 10
Match: AT1G62290.2 (Saposin-like aspartyl protease family protein )

HSP 1 Score: 677.9 bits (1748), Expect = 6.2e-195
Identity = 328/516 (63.57%), Postives = 406/516 (78.68%), Query Frame = 0

Query: 2   RKISFSHLLVSLLLLILHYSSEATSASNEGFLRIGLKKIKYDQNSRFKALLESKKGEFLG 61
           R ++FS + VS LL    YS       N+G  R+GLKK+K D N+R      SK+ E L 
Sbjct: 6   RAVAFS-VFVSFLLFFTAYSKR-----NDGTFRVGLKKLKLDPNNRLATRFGSKQEEALR 65

Query: 62  SSVGKHNQWGNNL-EESKNADIVPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVP 121
           SS+  +N   NNL  +S +ADIVPLKNYLDAQYYGEI IGTPPQKFTVIFDTGSSNLWVP
Sbjct: 66  SSLRSYN---NNLGGDSGDADIVPLKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWVP 125

Query: 122 SAKCIFSLACFFHAKYQSGRSSTYKRNGTSAAIQYGSGAISGFFSYDNVQVGDVIVRNQE 181
           S KC FSL+C+FHAKY+S RSSTYK++G  AAI YGSG+ISGFFSYD V VGD++V++QE
Sbjct: 126 SGKCFFSLSCYFHAKYKSSRSSTYKKSGKRAAIHYGSGSISGFFSYDAVTVGDLVVKDQE 185

Query: 182 LIEATSMSTMTFMAAKFDGILGLGFQEIATGGAVPVWYNMVKQKLVKEQVFSFWLNRNAE 241
            IE TS   +TF+ AKFDG+LGLGFQEIA G A PVWYNM+KQ L+K  VFSFWLNR+ +
Sbjct: 186 FIETTSEPGLTFLVAKFDGLLGLGFQEIAVGNATPVWYNMLKQGLIKRPVFSFWLNRDPK 245

Query: 242 EKEGGELVFGGVDPKHFKGQHTYVPVTDKGYWQFDIGDILIGGETTKYCAGGCSAIADSG 301
            +EGGE+VFGGVDPKHF+G+HT+VPVT +GYWQFD+G++LI GE+T YC  GCSAIADSG
Sbjct: 246 SEEGGEIVFGGVDPKHFRGEHTFVPVTQRGYWQFDMGEVLIAGESTGYCGSGCSAIADSG 305

Query: 302 TSLLAGPSNIVVSINRAIGAAAVAHPECKAIVSQYGRAIMDLLLAKAQPEKICSKIGVCT 361
           TSLLAGP+ +V  IN+AIGA+ V   +CK +V QYG+ I+DLLLA+ QP+KICS+IG+C 
Sbjct: 306 TSLLAGPTAVVAMINKAIGASGVVSQQCKTVVDQYGQTILDLLLAETQPKKICSQIGLCA 365

Query: 362 FDETHDVSLKIENVVSDKDGRSSGGFSEAMCSACEMAVLWIQDELKQNKTQEDIIENVNE 421
           +D TH VS+ IE+VV  ++ RSS G  +A C ACEMAV+WIQ +L+QN TQE I+  +NE
Sbjct: 366 YDGTHGVSMGIESVVDKENTRSSSGLRDAGCPACEMAVVWIQSQLRQNMTQERIVNYINE 425

Query: 422 LCDRGLNQD-ETLVDCGRISQMPNVSFTIGDRLFELTSKDYILKVGEGSAAQCISGFIPF 481
           +C+R  + + E+ VDC ++S+MP VSFTIG ++F+L  ++Y+LK+GEG  AQCISGF   
Sbjct: 426 ICERMPSPNGESAVDCSQLSKMPTVSFTIGGKVFDLAPEEYVLKIGEGPVAQCISGFTAL 485

Query: 482 DIPPPRGPLWILGDVFMGPYHTVFDFGKARVGFAEA 516
           DIPPPRGPLWILGDVFMG YHTVFDFG  +VGFAEA
Sbjct: 486 DIPPPRGPLWILGDVFMGKYHTVFDFGNEQVGFAEA 512

BLAST of CsGy6G032920 vs. TAIR 10
Match: AT1G11910.1 (aspartic proteinase A1 )

HSP 1 Score: 659.8 bits (1701), Expect = 1.8e-189
Identity = 323/509 (63.46%), Postives = 392/509 (77.01%), Query Frame = 0

Query: 9   LLVSLLLLILHYSSEATSASNEGFLRIGLKKIKYDQNSRFKALLESKKGEFLGSSVGKHN 68
           L+VS LL        A +  N+G  R+GLKK+K D  +R  A +ESK+ + L +      
Sbjct: 12  LIVSFLLCF-----SAFAERNDGTFRVGLKKLKLDSKNRLAARVESKQEKPLRA------ 71

Query: 69  QWGNNLEESKNADIVPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSAKCIFSL 128
                L +S +AD+V LKNYLDAQYYGEI IGTPPQKFTV+FDTGSSNLWVPS+KC FSL
Sbjct: 72  ---YRLGDSGDADVVVLKNYLDAQYYGEIAIGTPPQKFTVVFDTGSSNLWVPSSKCYFSL 131

Query: 129 ACFFHAKYQSGRSSTYKRNGTSAAIQYGSGAISGFFSYDNVQVGDVIVRNQELIEATSMS 188
           AC  H KY+S RSSTY++NG +AAI YG+GAI+GFFS D V VGD++V++QE IEAT   
Sbjct: 132 ACLLHPKYKSSRSSTYEKNGKAAAIHYGTGAIAGFFSNDAVTVGDLVVKDQEFIEATKEP 191

Query: 189 TMTFMAAKFDGILGLGFQEIATGGAVPVWYNMVKQKLVKEQVFSFWLNRNAEEKEGGELV 248
            +TF+ AKFDGILGLGFQEI+ G A PVWYNM+KQ L+KE VFSFWLNRNA+E+EGGELV
Sbjct: 192 GITFVVAKFDGILGLGFQEISVGKAAPVWYNMLKQGLIKEPVFSFWLNRNADEEEGGELV 251

Query: 249 FGGVDPKHFKGQHTYVPVTDKGYWQFDIGDILIGGETTKYCAGGCSAIADSGTSLLAGPS 308
           FGGVDP HFKG+HTYVPVT KGYWQFD+GD+LIGG  T +C  GCSAIADSGTSLLAGP+
Sbjct: 252 FGGVDPNHFKGKHTYVPVTQKGYWQFDMGDVLIGGAPTGFCESGCSAIADSGTSLLAGPT 311

Query: 309 NIVVSINRAIGAAAVAHPECKAIVSQYGRAIMDLLLAKAQPEKICSKIGVCTFDETHDVS 368
            I+  IN AIGAA V   +CK +V QYG+ I+DLLL++ QP+KICS+IG+CTFD T  VS
Sbjct: 312 TIITMINHAIGAAGVVSQQCKTVVDQYGQTILDLLLSETQPKKICSQIGLCTFDGTRGVS 371

Query: 369 LKIENVVSDKDGRSSGGFSEAMCSACEMAVLWIQDELKQNKTQEDIIENVNELCDR-GLN 428
           + IE+VV  ++ + S G  +A CSACEMAV+WIQ +L+QN TQE I+  VNELC+R    
Sbjct: 372 MGIESVVDKENAKLSNGVGDAACSACEMAVVWIQSQLRQNMTQERILNYVNELCERLPSP 431

Query: 429 QDETLVDCGRISQMPNVSFTIGDRLFELTSKDYILKVGEGSAAQCISGFIPFDIPPPRGP 488
             E+ VDC ++S MP VS TIG ++F+L  ++Y+LKVGEG  AQCISGFI  D+ PPRGP
Sbjct: 432 MGESAVDCAQLSTMPTVSLTIGGKVFDLAPEEYVLKVGEGPVAQCISGFIALDVAPPRGP 491

Query: 489 LWILGDVFMGPYHTVFDFGKARVGFAEAA 517
           LWILGDVFMG YHTVFDFG  +VGFAEAA
Sbjct: 492 LWILGDVFMGKYHTVFDFGNEQVGFAEAA 506

BLAST of CsGy6G032920 vs. TAIR 10
Match: AT4G04460.1 (Saposin-like aspartyl protease family protein )

HSP 1 Score: 630.9 bits (1626), Expect = 8.7e-181
Identity = 305/511 (59.69%), Postives = 393/511 (76.91%), Query Frame = 0

Query: 7   SHLLVSLLLLILHYSSEATSASNEGFLRIGLKKIKYDQNSRFKALLESKKGEFLGSSVGK 66
           S LLV LL  ++  S+ +   + +G +RIGLKK K D+++R  + L  K     GS    
Sbjct: 7   SFLLVFLLSCLILISTASCERNGDGTIRIGLKKRKLDRSNRLASQLFLKN---RGSHWSP 66

Query: 67  HNQWGNNLEESKNADIVPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSAKCIF 126
            + +  N    +NAD+VPLKNYLDAQYYG+I IGTPPQKFTVIFDTGSSNLW+PS KC  
Sbjct: 67  KHYFRLN---DENADMVPLKNYLDAQYYGDITIGTPPQKFTVIFDTGSSNLWIPSTKCYL 126

Query: 127 SLACFFHAKYQSGRSSTYKRNGTSAAIQYGSGAISGFFSYDNVQVGDVIVRNQELIEATS 186
           S+AC+FH+KY++ +SS+Y++NG  A+I+YG+GAISG+FS D+V+VGD++V+ QE IEATS
Sbjct: 127 SVACYFHSKYKASQSSSYRKNGKPASIRYGTGAISGYFSNDDVKVGDIVVKEQEFIEATS 186

Query: 187 MSTMTFMAAKFDGILGLGFQEIATGGAVPVWYNMVKQKLVKEQVFSFWLNRNAEEKEGGE 246
              +TF+ AKFDGILGLGF+EI+ G + PVWYNMV++ LVKE +FSFWLNRN ++ EGGE
Sbjct: 187 EPGITFLLAKFDGILGLGFKEISVGNSTPVWYNMVEKGLVKEPIFSFWLNRNPKDPEGGE 246

Query: 247 LVFGGVDPKHFKGQHTYVPVTDKGYWQFDIGDILIGGETTKYCAGGCSAIADSGTSLLAG 306
           +VFGGVDPKHFKG+HT+VPVT KGYWQFD+GD+ I G+ T YCA GCSAIADSGTSLL G
Sbjct: 247 IVFGGVDPKHFKGEHTFVPVTHKGYWQFDMGDLQIAGKPTGYCAKGCSAIADSGTSLLTG 306

Query: 307 PSNIVVSINRAIGAAAVAHPECKAIVSQYGRAIMDLLLAKAQPEKICSKIGVCTFDETHD 366
           PS ++  IN AIGA  +   ECKA+V QYG+ +++ LLA+  P+K+CS+IGVC +D T  
Sbjct: 307 PSTVITMINHAIGAQGIVSRECKAVVDQYGKTMLNSLLAQEDPKKVCSQIGVCAYDGTQS 366

Query: 367 VSLKIENVVSDKDGRSSGGFSEAMCSACEMAVLWIQDELKQNKTQEDIIENVNELCDRGL 426
           VS+ I++VV D    +SG  ++AMCSACEMA +W++ EL QN+TQE I+    ELCD   
Sbjct: 367 VSMGIQSVVDD---GTSGLLNQAMCSACEMAAVWMESELTQNQTQERILAYAAELCDHIP 426

Query: 427 NQD-ETLVDCGRISQMPNVSFTIGDRLFELTSKDYILKVGEGSAAQCISGFIPFDIPPPR 486
            Q+ ++ VDCGR+S MP V+F+IG R F+LT +DYI K+GEG  +QC SGF   DI PPR
Sbjct: 427 TQNQQSAVDCGRVSSMPIVTFSIGGRSFDLTPQDYIFKIGEGVESQCTSGFTAMDIAPPR 486

Query: 487 GPLWILGDVFMGPYHTVFDFGKARVGFAEAA 517
           GPLWILGD+FMGPYHTVFD+GK RVGFA+AA
Sbjct: 487 GPLWILGDIFMGPYHTVFDYGKGRVGFAKAA 508

BLAST of CsGy6G032920 vs. TAIR 10
Match: AT4G04460.2 (Saposin-like aspartyl protease family protein )

HSP 1 Score: 622.5 bits (1604), Expect = 3.1e-178
Identity = 304/511 (59.49%), Postives = 391/511 (76.52%), Query Frame = 0

Query: 7   SHLLVSLLLLILHYSSEATSASNEGFLRIGLKKIKYDQNSRFKALLESKKGEFLGSSVGK 66
           S LLV LL  ++  S+ +   + +G +RIGLKK K D+++R  + L  K     GS    
Sbjct: 7   SFLLVFLLSCLILISTASCERNGDGTIRIGLKKRKLDRSNRLASQLFLKN---RGSHWSP 66

Query: 67  HNQWGNNLEESKNADIVPLKNYLDAQYYGEIGIGTPPQKFTVIFDTGSSNLWVPSAKCIF 126
            + +  N    +NAD+VPLKNYLDAQYYG+I IGTPPQKFTVIFDTGSSNLW+PS KC  
Sbjct: 67  KHYFRLN---DENADMVPLKNYLDAQYYGDITIGTPPQKFTVIFDTGSSNLWIPSTKCYL 126

Query: 127 SLACFFHAKYQSGRSSTYKRNGTSAAIQYGSGAISGFFSYDNVQVGDVIVRNQELIEATS 186
           S+AC+FH+KY++ +SS+Y++NG  A+I+YG+GAISG+FS D+V+VGD++V+ QE IEATS
Sbjct: 127 SVACYFHSKYKASQSSSYRKNGKPASIRYGTGAISGYFSNDDVKVGDIVVKEQEFIEATS 186

Query: 187 MSTMTFMAAKFDGILGLGFQEIATGGAVPVWYNMVKQKLVKEQVFSFWLNRNAEEKEGGE 246
              +TF+ AKFDGILGLGF+EI+ G + PVWYNMV++ LVKE +FSFWLNRN ++ EGGE
Sbjct: 187 EPGITFLLAKFDGILGLGFKEISVGNSTPVWYNMVEKGLVKEPIFSFWLNRNPKDPEGGE 246

Query: 247 LVFGGVDPKHFKGQHTYVPVTDKGYWQFDIGDILIGGETTKYCAGGCSAIADSGTSLLAG 306
           +VFGGVDPKHFKG+HT+VPVT KGYWQFD+GD+ I G+ T YCA GCSAIADSGTSLL G
Sbjct: 247 IVFGGVDPKHFKGEHTFVPVTHKGYWQFDMGDLQIAGKPTGYCAKGCSAIADSGTSLLTG 306

Query: 307 PSNIVVSINRAIGAAAVAHPECKAIVSQYGRAIMDLLLAKAQPEKICSKIGVCTFDETHD 366
           PS ++  IN AIGA  +   ECKA+V QYG+ +++ LLA    +K+CS+IGVC +D T  
Sbjct: 307 PSTVITMINHAIGAQGIVSRECKAVVDQYGKTMLNSLLA----QKVCSQIGVCAYDGTQS 366

Query: 367 VSLKIENVVSDKDGRSSGGFSEAMCSACEMAVLWIQDELKQNKTQEDIIENVNELCDRGL 426
           VS+ I++VV D    +SG  ++AMCSACEMA +W++ EL QN+TQE I+    ELCD   
Sbjct: 367 VSMGIQSVVDD---GTSGLLNQAMCSACEMAAVWMESELTQNQTQERILAYAAELCDHIP 426

Query: 427 NQD-ETLVDCGRISQMPNVSFTIGDRLFELTSKDYILKVGEGSAAQCISGFIPFDIPPPR 486
            Q+ ++ VDCGR+S MP V+F+IG R F+LT +DYI K+GEG  +QC SGF   DI PPR
Sbjct: 427 TQNQQSAVDCGRVSSMPIVTFSIGGRSFDLTPQDYIFKIGEGVESQCTSGFTAMDIAPPR 486

Query: 487 GPLWILGDVFMGPYHTVFDFGKARVGFAEAA 517
           GPLWILGD+FMGPYHTVFD+GK RVGFA+AA
Sbjct: 487 GPLWILGDIFMGPYHTVFDYGKGRVGFAKAA 504

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O040575.9e-19866.14Aspartic proteinase OS=Cucurbita pepo OX=3663 PE=2 SV=1[more]
Q8VYL38.8e-19463.57Aspartic proteinase A2 OS=Arabidopsis thaliana OX=3702 GN=APA2 PE=2 SV=1[more]
O653902.5e-18863.46Aspartic proteinase A1 OS=Arabidopsis thaliana OX=3702 GN=APA1 PE=1 SV=1[more]
Q424563.3e-18562.40Aspartic proteinase oryzasin-1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g... [more]
P422106.3e-18461.45Phytepsin OS=Hordeum vulgare OX=4513 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_004134774.10.0100.00aspartic proteinase [Cucumis sativus] >XP_011658133.1 aspartic proteinase [Cucum... [more]
XP_008440021.10.093.02PREDICTED: aspartic proteinase-like isoform X1 [Cucumis melo][more]
XP_008440022.10.093.02PREDICTED: aspartic proteinase-like isoform X2 [Cucumis melo] >XP_008440023.1 PR... [more]
TYK13029.10.093.02aspartic proteinase-like isoform X2 [Cucumis melo var. makuwa][more]
XP_038880988.10.087.40aspartic proteinase-like isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A0A0KMZ90.0100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G513550 PE=3 SV=1[more]
A0A1S3B0400.093.02aspartic proteinase-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103484625 PE=3... [more]
A0A1S3B0580.093.02aspartic proteinase-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103484625 PE=3... [more]
A0A5D3CRY90.093.02Aspartic proteinase-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A6J1IKS16.83e-31282.42aspartic proteinase-like OS=Cucurbita maxima OX=3661 GN=LOC111477945 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G62290.16.2e-19563.57Saposin-like aspartyl protease family protein [more]
AT1G62290.26.2e-19563.57Saposin-like aspartyl protease family protein [more]
AT1G11910.11.8e-18963.46aspartic proteinase A1 [more]
AT4G04460.18.7e-18159.69Saposin-like aspartyl protease family protein [more]
AT4G04460.23.1e-17859.49Saposin-like aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001461Aspartic peptidase A1 familyPRINTSPR00792PEPSINcoord: 99..119
score: 73.01
coord: 245..258
score: 44.27
coord: 295..306
score: 58.07
coord: 489..504
score: 58.48
IPR001461Aspartic peptidase A1 familyPANTHERPTHR47966BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATEDcoord: 9..516
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 33..251
e-value: 1.8E-76
score: 258.6
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 252..516
e-value: 1.6E-106
score: 357.0
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 13..515
NoneNo IPR availableGENE3D1.10.225.10coord: 323..426
e-value: 1.6E-106
score: 357.0
NoneNo IPR availablePANTHERPTHR47966:SF31ASPARTIC PROTEINASE-LIKEcoord: 9..516
IPR007856Saposin-like type B, region 1PFAMPF05184SapB_1coord: 390..424
e-value: 7.3E-11
score: 41.9
IPR008138Saposin B type, region 2PFAMPF03489SapB_2coord: 327..359
e-value: 2.7E-11
score: 43.5
IPR033121Peptidase family A1 domainPFAMPF00026Aspcoord: 92..515
e-value: 1.9E-127
score: 425.7
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 93..513
score: 71.829689
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 108..119
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 295..306
IPR008139Saposin B type domainPROSITEPS50015SAP_Bcoord: 387..428
score: 9.954399
IPR008139Saposin B type domainPROSITEPS50015SAP_Bcoord: 323..363
score: 12.231999
IPR033869PhytepsinCDDcd06098phytepsincoord: 83..514
e-value: 0.0
score: 605.136
IPR011001Saposin-likeSUPERFAMILY47862Saposincoord: 325..423

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy6G032920.1CsGy6G032920.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004190 aspartic-type endopeptidase activity