CsGy6G032260 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy6G032260
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionATP-dependent 6-phosphofructokinase
LocationGy14Chr6: 29575729 .. 29578089 (+)
RNA-Seq ExpressionCsGy6G032260
SyntenyCsGy6G032260
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATTCTCCCAACTTTTATTCCCATAACTACTTTACGCCATTTTACACTCCATTCTCAATTTCCCCTCTTTCTCTCCGATGGCCACCGCCAAAATATCCGACCAACACCCCACAATCACCGTCGCAGACCTCCCCCACCTGACCGACAACTTCCCCAATCTCAAAACCCGAACCAACCCACTCCAAACCAACCAATTCTTCCACCCAATCGACGGCTTCTACGCTACCTCCACCGACATCGTCCTCCGCCGGATCGCCTTCGACCTGTCCGGAGACACTTCCCCTGCTCACGTGGCGTACCGCCGGGCTGGTCCGAGGAGGAAGGTTTATTTCGAACCGGAGTCGGTTCGAGCGGCGATCGTGACATGCGGAGGGCTGTGCCCCGGGATGAATACGGTGATCAGAGAGCTGGTGGTGGGGCTGTGGGAACTTTACGGAGTGCGGCAGATATATGGGATTGTGGCGGGTTACAGAGGGTTTTATTCGACGGAGCCCGTGGAATTGAATTTGAAATTGGTTGATAATTGGCATAAGAGAGGTGGGACTGCTCTTCAAACATCACGAGGAGGGTTTGATCTGAAGAAGATAGTGGATGCCATAGAAAACCATGGTTTCAATCAGGTTTTTACTCTGTTTCTAACTGAATCTTTACGTCTCTGTCTTTTCAACTGCAAATTAAGTGTTTTGTAGCGGTGGATGACGTGTTTATATATATATAAAAGAATAATTAGGTGTATTTATACACAAGCTGAGTAATTTAGAAGAGTAGAATTGGATGGGTAAGAAGAAAAACCACGAACTCAAATATTATTCAATACGAATTATATACATGCAGTGGAGTAAGCTCCTCGATGACACAATTGAGAACCATGTTATTTTTAAATATAACCAAATAAAAATAGATAGAAATATCGAAACTAATTAGGAAAATACACTTATTCGATGCAATTTTCCTCAATATATATATATACAAAATACAACCAAATTTTAAATTCTAATAGTTTAGTTTATGTCTATAAATGTATATTTTATAAATATATTTTTTCAGTAGTTTTGGGGTGATTGGAATATTCTTTTGAATTTTGTGTAAAGTAGTTTTGTAAAATGATTAAAAATAACAAATTTGACAAAATATTTATCAACTAACACAATTTTATATTCTATCTCTACCAAACATTTAATAGTCATTAATAATTAATAAGAATCTAAGAATTTTGGTGTAAATTATAAATATTTTAACTTATTTTGTTATTTTTAAAAACATTATAATATTTTTCAGATTTATCAATTAATTTTCTGTTTAGTTTATTTTAGAATATAGTTTTTTCGGTGTATTTGTTAAATAATTTATTTTTCATACGAATTCTGTTATGCCTTCCAACTCCAACCGTAAAACTTGGATCTTTCCATGTTCAAGCAGTTTCAACTTTCAATTACGAATTATGATTGTTTGAATGGCAATTCCTTGCAAAATGAGGTAGGTTTACATAACCGGAGGGGATGGCACAATGCGTGGCGCCGTCAAGATATTCGAGGAAGTACGTCGTCGGAATCTTCAAGTCGCGGTCACCGGAATTCCTAAAACTGTCGACAATGACATTGGAATTATTGACCGATCATTCGGATTCCAAACGGCCGTCGAAATGGCACAACAAGCAATCAACGCTGCTCATGTGGAGGCCGAGAGCGCCGTGAATGGGATAGGGCTAGTGAAATTGATGGGCAGAAGCACCGGCCATATTGCACTCCATGCCACATTAAGCAGCCGAGACGTCGATTGCTGTTTGATACCTGAAATGGCCTTCTATTTGGAAGGCAACGGAGGTTTGTTTCCGTTTCTTGAACGAAGGTTGAAGGAGAACGGGCATGCGGTTCTGGTGGTTGCTGAGGGAGCAGGACAAGGTATGATACCTAGAACGGAAGCGCAAAAGGAAGAGAGAGACGAATCTGGAAATCCGGTGTTCTTGGATGTAGGCGGATGGTTGAAGACGGAGCTCAAGAATTGGTGGGCGACAACGCATCCCGGTGAGTTATTTACTGTGAAATACATAGATCCTACTTATATGATTCGTGCCGTGCCTGCAAATGCTACAGATAATTCCTATTGTACACTGCTCGCTCATTCAGCCATTCATGGAGTAATGGCTGGTTATACTGGATTTGTCTCTGGCCCTATTAATGGAAACTATTCATATATTCCATTGAAAGAGGTGGCAGAAGCAAAGAATGAAGTTAATACTAGAGATCATAAATGGGCATGGGTTAGATCTGTCACTAATCAGCCCGATTTTGTTAAAAGTTAACTACTCGATCTGAAAAACATCAGTCCATAAGGGGGTCTTAGTGGAAATGAAATTTTAG

mRNA sequence

AAATTCTCCCAACTTTTATTCCCATAACTACTTTACGCCATTTTACACTCCATTCTCAATTTCCCCTCTTTCTCTCCGATGGCCACCGCCAAAATATCCGACCAACACCCCACAATCACCGTCGCAGACCTCCCCCACCTGACCGACAACTTCCCCAATCTCAAAACCCGAACCAACCCACTCCAAACCAACCAATTCTTCCACCCAATCGACGGCTTCTACGCTACCTCCACCGACATCGTCCTCCGCCGGATCGCCTTCGACCTGTCCGGAGACACTTCCCCTGCTCACGTGGCGTACCGCCGGGCTGGTCCGAGGAGGAAGGTTTATTTCGAACCGGAGTCGGTTCGAGCGGCGATCGTGACATGCGGAGGGCTGTGCCCCGGGATGAATACGGTGATCAGAGAGCTGGTGGTGGGGCTGTGGGAACTTTACGGAGTGCGGCAGATATATGGGATTGTGGCGGGTTACAGAGGGTTTTATTCGACGGAGCCCGTGGAATTGAATTTGAAATTGGTTGATAATTGGCATAAGAGAGGTGGGACTGCTCTTCAAACATCACGAGGAGGGTTTGATCTGAAGAAGATAGTGGATGCCATAGAAAACCATGGTTTCAATCAGGTTTACATAACCGGAGGGGATGGCACAATGCGTGGCGCCGTCAAGATATTCGAGGAAGTACGTCGTCGGAATCTTCAAGTCGCGGTCACCGGAATTCCTAAAACTGTCGACAATGACATTGGAATTATTGACCGATCATTCGGATTCCAAACGGCCGTCGAAATGGCACAACAAGCAATCAACGCTGCTCATGTGGAGGCCGAGAGCGCCGTGAATGGGATAGGGCTAGTGAAATTGATGGGCAGAAGCACCGGCCATATTGCACTCCATGCCACATTAAGCAGCCGAGACGTCGATTGCTGTTTGATACCTGAAATGGCCTTCTATTTGGAAGGCAACGGAGGTTTGTTTCCGTTTCTTGAACGAAGGTTGAAGGAGAACGGGCATGCGGTTCTGGTGGTTGCTGAGGGAGCAGGACAAGGTATGATACCTAGAACGGAAGCGCAAAAGGAAGAGAGAGACGAATCTGGAAATCCGGTGTTCTTGGATGTAGGCGGATGGTTGAAGACGGAGCTCAAGAATTGGTGGGCGACAACGCATCCCGGTGAGTTATTTACTGTGAAATACATAGATCCTACTTATATGATTCGTGCCGTGCCTGCAAATGCTACAGATAATTCCTATTGTACACTGCTCGCTCATTCAGCCATTCATGGAGTAATGGCTGGTTATACTGGATTTGTCTCTGGCCCTATTAATGGAAACTATTCATATATTCCATTGAAAGAGGTGGCAGAAGCAAAGAATGAAGTTAATACTAGAGATCATAAATGGGCATGGGTTAGATCTGTCACTAATCAGCCCGATTTTGTTAAAAGTTAACTACTCGATCTGAAAAACATCAGTCCATAAGGGGGTCTTAGTGGAAATGAAATTTTAG

Coding sequence (CDS)

ATGGCCACCGCCAAAATATCCGACCAACACCCCACAATCACCGTCGCAGACCTCCCCCACCTGACCGACAACTTCCCCAATCTCAAAACCCGAACCAACCCACTCCAAACCAACCAATTCTTCCACCCAATCGACGGCTTCTACGCTACCTCCACCGACATCGTCCTCCGCCGGATCGCCTTCGACCTGTCCGGAGACACTTCCCCTGCTCACGTGGCGTACCGCCGGGCTGGTCCGAGGAGGAAGGTTTATTTCGAACCGGAGTCGGTTCGAGCGGCGATCGTGACATGCGGAGGGCTGTGCCCCGGGATGAATACGGTGATCAGAGAGCTGGTGGTGGGGCTGTGGGAACTTTACGGAGTGCGGCAGATATATGGGATTGTGGCGGGTTACAGAGGGTTTTATTCGACGGAGCCCGTGGAATTGAATTTGAAATTGGTTGATAATTGGCATAAGAGAGGTGGGACTGCTCTTCAAACATCACGAGGAGGGTTTGATCTGAAGAAGATAGTGGATGCCATAGAAAACCATGGTTTCAATCAGGTTTACATAACCGGAGGGGATGGCACAATGCGTGGCGCCGTCAAGATATTCGAGGAAGTACGTCGTCGGAATCTTCAAGTCGCGGTCACCGGAATTCCTAAAACTGTCGACAATGACATTGGAATTATTGACCGATCATTCGGATTCCAAACGGCCGTCGAAATGGCACAACAAGCAATCAACGCTGCTCATGTGGAGGCCGAGAGCGCCGTGAATGGGATAGGGCTAGTGAAATTGATGGGCAGAAGCACCGGCCATATTGCACTCCATGCCACATTAAGCAGCCGAGACGTCGATTGCTGTTTGATACCTGAAATGGCCTTCTATTTGGAAGGCAACGGAGGTTTGTTTCCGTTTCTTGAACGAAGGTTGAAGGAGAACGGGCATGCGGTTCTGGTGGTTGCTGAGGGAGCAGGACAAGGTATGATACCTAGAACGGAAGCGCAAAAGGAAGAGAGAGACGAATCTGGAAATCCGGTGTTCTTGGATGTAGGCGGATGGTTGAAGACGGAGCTCAAGAATTGGTGGGCGACAACGCATCCCGGTGAGTTATTTACTGTGAAATACATAGATCCTACTTATATGATTCGTGCCGTGCCTGCAAATGCTACAGATAATTCCTATTGTACACTGCTCGCTCATTCAGCCATTCATGGAGTAATGGCTGGTTATACTGGATTTGTCTCTGGCCCTATTAATGGAAACTATTCATATATTCCATTGAAAGAGGTGGCAGAAGCAAAGAATGAAGTTAATACTAGAGATCATAAATGGGCATGGGTTAGATCTGTCACTAATCAGCCCGATTTTGTTAAAAGTTAA

Protein sequence

MATAKISDQHPTITVADLPHLTDNFPNLKTRTNPLQTNQFFHPIDGFYATSTDIVLRRIAFDLSGDTSPAHVAYRRAGPRRKVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGVRQIYGIVAGYRGFYSTEPVELNLKLVDNWHKRGGTALQTSRGGFDLKKIVDAIENHGFNQVYITGGDGTMRGAVKIFEEVRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAINAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGNGGLFPFLERRLKENGHAVLVVAEGAGQGMIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWWATTHPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVKS*
Homology
BLAST of CsGy6G032260 vs. ExPASy Swiss-Prot
Match: Q9FIK0 (ATP-dependent 6-phosphofructokinase 2 OS=Arabidopsis thaliana OX=3702 GN=PFK2 PE=1 SV=1)

HSP 1 Score: 697.2 bits (1798), Expect = 1.2e-199
Identity = 334/438 (76.26%), Postives = 378/438 (86.30%), Query Frame = 0

Query: 14  TVADLPHLTDNFPNLKTRTNPLQTNQFFHPIDGFYATSTDIVLRRIAFDLSGDTSPAHVA 73
           ++  LP L+     L+ R NPL+ N +FHP +GFY T +D++L ++A+D S   S + VA
Sbjct: 6   SIRKLPSLS----GLRHRRNPLEDNPYFHPSNGFYITPSDVILAQVAYDHSAH-SQSRVA 65

Query: 74  YRRAGPRRKVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGVRQIYGIVAGYRG 133
           Y RAGPRR++ +EP +V+AAIVTCGGLCPGMNTVIRELVVGLWELYGVR+IYGI AGYRG
Sbjct: 66  YHRAGPRREIMYEPSAVKAAIVTCGGLCPGMNTVIRELVVGLWELYGVREIYGIPAGYRG 125

Query: 134 FYSTEPVELNLKLVDNWHKRGGTALQTSRGGFDLKKIVDAIENHGFNQVYITGGDGTMRG 193
           FYS + V+L+ K V +WHK+GGT L TSRGGF L+KIVDAI  +G+NQVYI GGDGTMRG
Sbjct: 126 FYSMKAVKLDPKAVHDWHKKGGTVLATSRGGFHLQKIVDAIHLNGYNQVYIIGGDGTMRG 185

Query: 194 AVKIFEEVRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAINAAHVEAESAVN 253
           AV+IF+E+  R L+V +T IPKTVDND+GIIDRSFGFQTAVEMAQ+AI+AAHVEAESAVN
Sbjct: 186 AVEIFKEISLRKLEVGITVIPKTVDNDVGIIDRSFGFQTAVEMAQEAISAAHVEAESAVN 245

Query: 254 GIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGNGGLFPFLERRLKENGHAVL 313
           GIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEM FYLEG GGLF FLE+RLKE GHAVL
Sbjct: 246 GIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMDFYLEGKGGLFEFLEKRLKERGHAVL 305

Query: 314 VVAEGAGQGMIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWWATTHPGELFTVKYIDP 373
           VVAEGAGQ MIPR E+QK+ERDESGN VFLDVG W K+ LK WW   HP ELFTVKYIDP
Sbjct: 306 VVAEGAGQEMIPRNESQKQERDESGNAVFLDVGVWFKSVLKAWWEREHPDELFTVKYIDP 365

Query: 374 TYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIPLKEVAEAKNEVN 433
           TYMIRAVPANATDN YCTLLAHSAIHGVMAGYTGFV GPINGNY+YIPL+EVA+ KN+VN
Sbjct: 366 TYMIRAVPANATDNLYCTLLAHSAIHGVMAGYTGFVPGPINGNYAYIPLEEVAQTKNQVN 425

Query: 434 TRDHKWAWVRSVTNQPDF 452
           TRDHKWAWVRSVTNQPDF
Sbjct: 426 TRDHKWAWVRSVTNQPDF 438

BLAST of CsGy6G032260 vs. ExPASy Swiss-Prot
Match: Q94AA4 (ATP-dependent 6-phosphofructokinase 3 OS=Arabidopsis thaliana OX=3702 GN=PFK3 PE=1 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 4.6e-146
Identity = 254/436 (58.26%), Postives = 318/436 (72.94%), Query Frame = 0

Query: 17  DLPHLTDNFPNLKTRTNPLQTNQFFHPIDGFYATSTDIVLRRIAFDLSGDTSPAHVAYRR 76
           D+PHL+D  P L T  NPLQ N  +  +  ++  + D V ++I     G   P  + +RR
Sbjct: 22  DVPHLSDYLPGLPTYPNPLQDNPAYSVVKQYFVDADDSVPQKIVVHKDG---PRGIHFRR 81

Query: 77  AGPRRKVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGVRQIYGIVAGYRGFYS 136
           AGPR+KVYFE + V A IVTCGGLCPG+NTVIRE+V  L  +YGV++I GI  GYRGFY+
Sbjct: 82  AGPRQKVYFESDEVHACIVTCGGLCPGLNTVIREIVSSLSYMYGVKRILGIDGGYRGFYA 141

Query: 137 TEPVELNLKLVDNWHKRGGTALQTSRGGFDLKKIVDAIENHGFNQVYITGGDGTMRGAVK 196
              V L+ K+V++ HKRGGT L TSRGG D  KIVD+I++ G NQVYI GGDGT RGA  
Sbjct: 142 KNTVSLDSKVVNDIHKRGGTILGTSRGGHDTTKIVDSIQDRGINQVYIIGGDGTQRGASV 201

Query: 197 IFEEVRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAINAAHVEAESAVNGIG 256
           IFEE+RRR L+VAV GIPKT+DNDI +ID+SFGF TAVE AQ+AINAAHVEAES  NGIG
Sbjct: 202 IFEEIRRRGLKVAVIGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHVEAESIENGIG 261

Query: 257 LVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGNGGLFPFLERRLKENGHAVLVVA 316
           +VKLMGR +G IA++ATL+SRDVDCCLIPE  FYLEG GGLF ++E+RLKE+GH VLV+A
Sbjct: 262 VVKLMGRYSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFEYIEKRLKESGHMVLVIA 321

Query: 317 EGAGQGMIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWWATTHPGELFTVKYIDPTYM 376
           EGAGQ ++ ++      +D SGN +  DVG WL   +K+ +       +  +KYIDPTYM
Sbjct: 322 EGAGQDLMSKSMESMTLKDASGNKLLKDVGLWLSQSIKDHF--NQKKMVMNLKYIDPTYM 381

Query: 377 IRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIPLKEVAEAKNEVNTRD 436
           IRAVP+NA+DN YCTLLA SA+HG MAGYTG++SG +NG  +YIP   + E +N V   D
Sbjct: 382 IRAVPSNASDNVYCTLLAQSAVHGAMAGYTGYISGLVNGRQTYIPFYRITEKQNHVVITD 441

Query: 437 HKWAWVRSVTNQPDFV 453
             WA + S TNQP F+
Sbjct: 442 RMWARLLSSTNQPSFL 452

BLAST of CsGy6G032260 vs. ExPASy Swiss-Prot
Match: Q9C5J7 (ATP-dependent 6-phosphofructokinase 7 OS=Arabidopsis thaliana OX=3702 GN=PFK7 PE=1 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 1.1e-144
Identity = 253/436 (58.03%), Postives = 316/436 (72.48%), Query Frame = 0

Query: 17  DLPHLTDNFPNLKTRTNPLQTNQFFHPIDGFYATSTDIVLRRIAFDLSGDTSPAHVAYRR 76
           D+PHL D  P+L T  NPLQ N  +  +  ++  + D V  ++     G   P  V +RR
Sbjct: 22  DVPHLIDYLPDLPTYPNPLQDNPAYSVVKQYFVHADDSVPEKVVVHKDG---PRGVHFRR 81

Query: 77  AGPRRKVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGVRQIYGIVAGYRGFYS 136
           AGPR+KVYFE + V A IVTCGGLCPG+NTVIRE+V  L  +YGV++I GI  GYRGFY+
Sbjct: 82  AGPRQKVYFESDEVHACIVTCGGLCPGLNTVIREVVSSLSYMYGVKRILGIDGGYRGFYA 141

Query: 137 TEPVELNLKLVDNWHKRGGTALQTSRGGFDLKKIVDAIENHGFNQVYITGGDGTMRGAVK 196
              + LN K+V++ HKRGGT + TSRGG D  KIVD+I++ G NQVYI GGDGT RGA  
Sbjct: 142 KNTIPLNSKVVNDIHKRGGTIIGTSRGGHDTNKIVDSIQDRGINQVYIIGGDGTQRGASV 201

Query: 197 IFEEVRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAINAAHVEAESAVNGIG 256
           IFEE+RRR L+VAV GIPKT+DNDI +ID+SFGF TAVE AQ+AINAAHVEAES  NGIG
Sbjct: 202 IFEEIRRRRLKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHVEAESNENGIG 261

Query: 257 LVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGNGGLFPFLERRLKENGHAVLVVA 316
            VKLMGR +G+IA++ATL+SRDVDCCLIPE  FYLEG GGLF F+ERRLK++GH V+V+A
Sbjct: 262 FVKLMGRYSGYIAMYATLASRDVDCCLIPESPFYLEGEGGLFEFIERRLKDHGHMVIVLA 321

Query: 317 EGAGQGMIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWWATTHPGELFTVKYIDPTYM 376
           EGAGQ ++ ++  +    D SGN +  DVG WL   +K+ +       +  +KYIDPTYM
Sbjct: 322 EGAGQDLMCKS-MESTPMDASGNKLLKDVGLWLSQSIKDHFKKNK--MVMNLKYIDPTYM 381

Query: 377 IRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIPLKEVAEAKNEVNTRD 436
           IRAVP+NA+DN YCTLLA SA+HG MAGYTG+ SG +NG  +YIP   + E +N V   D
Sbjct: 382 IRAVPSNASDNVYCTLLAQSAVHGAMAGYTGYTSGLVNGRQTYIPFYRITETQNNVVITD 441

Query: 437 HKWAWVRSVTNQPDFV 453
             WA + S TNQP F+
Sbjct: 442 RMWARLLSSTNQPSFL 451

BLAST of CsGy6G032260 vs. ExPASy Swiss-Prot
Match: Q9FKG3 (ATP-dependent 6-phosphofructokinase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PFK4 PE=1 SV=1)

HSP 1 Score: 511.9 bits (1317), Expect = 7.3e-144
Identity = 248/436 (56.88%), Postives = 313/436 (71.79%), Query Frame = 0

Query: 17  DLPHLTDNFPNLKTRTNPLQTNQFFHPIDGFYATSTDIVLRRIAFDLSGDTSPAHVAYRR 76
           D+PHLT   P+L +  NPL+ +Q +  +   + +S D+V + I        S   V +RR
Sbjct: 73  DVPHLTKFLPDLPSYPNPLKESQAYAIVKRTFVSSEDVVAQNIVVQKG---SKRGVHFRR 132

Query: 77  AGPRRKVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGVRQIYGIVAGYRGFYS 136
           AGPR +VYF  + V+A IVTCGGLCPG+NTVIRE+V GL  +YGV  I GI  GYRGFYS
Sbjct: 133 AGPRERVYFRSDEVKACIVTCGGLCPGINTVIREIVCGLNNMYGVNNILGIQGGYRGFYS 192

Query: 137 TEPVELNLKLVDNWHKRGGTALQTSRGGFDLKKIVDAIENHGFNQVYITGGDGTMRGAVK 196
              + L  K+V++ HKRGGT LQTSRGG D  KIVD I++ G NQVYI GG GT +GA K
Sbjct: 193 KNTMNLTPKVVNDIHKRGGTFLQTSRGGHDTAKIVDNIQDRGINQVYIIGGGGTQKGAEK 252

Query: 197 IFEEVRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAINAAHVEAESAVNGIG 256
           I+EEV RR LQVAV+GIPKT+DNDI +ID+SFGF TAVE AQ+AINAAHVE ES  NG+G
Sbjct: 253 IYEEVERRGLQVAVSGIPKTIDNDIAVIDKSFGFDTAVEEAQRAINAAHVEVESVENGVG 312

Query: 257 LVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGNGGLFPFLERRLKENGHAVLVVA 316
           +VKLMGR +G IA+ ATL++RDVDCCLIPE  F+LEG GGLF F+E RLKEN H V+V+A
Sbjct: 313 IVKLMGRYSGFIAMIATLANRDVDCCLIPESPFFLEGKGGLFEFIEERLKENRHMVIVIA 372

Query: 317 EGAGQGMIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWWATTHPGELFTVKYIDPTYM 376
           EGAGQ  + ++    E +D SGN + LDVG WL  ++K+ +       +  +KYIDPTYM
Sbjct: 373 EGAGQDYVAQSMRASETKDASGNRLLLDVGLWLTQQIKDHFTNVRK-MMINMKYIDPTYM 432

Query: 377 IRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIPLKEVAEAKNEVNTRD 436
           IRA+P+NA+DN YCTLLA SA+HG MAGY+GF  GP+N  ++YIP+ +V E  N V   D
Sbjct: 433 IRAIPSNASDNVYCTLLAQSAVHGAMAGYSGFTVGPVNSRHAYIPISQVTEVTNTVKLTD 492

Query: 437 HKWAWVRSVTNQPDFV 453
             WA + + TNQP F+
Sbjct: 493 RMWARLLASTNQPSFL 504

BLAST of CsGy6G032260 vs. ExPASy Swiss-Prot
Match: Q9M0F9 (ATP-dependent 6-phosphofructokinase 1 OS=Arabidopsis thaliana OX=3702 GN=PFK1 PE=1 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 2.8e-143
Identity = 251/440 (57.05%), Postives = 321/440 (72.95%), Query Frame = 0

Query: 17  DLPHLTDNFPNLKTRTNPLQTNQFFHPIDGFYATSTDIVLRRIAFDLSGDTSPAHVAYRR 76
           D+PH +D+FP+  T  NPLQ N  +  +  ++    D V ++I        SP    +RR
Sbjct: 23  DVPHFSDDFPDHPTYPNPLQDNAAYSVVKQYFVDEDDTVPQKIVVH---PDSPRGTHFRR 82

Query: 77  AGPRRKVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGVRQIYGIVAGYRGFYS 136
           AGPR++VYFE + V A IVTCGGLCPG+NTVIRE+V GL  +YGV++I GI  GYRGFY+
Sbjct: 83  AGPRQRVYFESDDVLACIVTCGGLCPGLNTVIREIVCGLSYMYGVKRILGIDGGYRGFYA 142

Query: 137 TEPVELNLKLVDNWHKRGGTALQTSRGGFDLKKIVDAIENHGFNQVYITGGDGTMRGAVK 196
              + L+LK V++ H+ GGT L TSRGG +  KIVD+I++ G NQVYI GGDG+ +GA  
Sbjct: 143 RNTIHLDLKTVNDIHRSGGTILGTSRGGHNTTKIVDSIQDRGINQVYIIGGDGSQKGAAA 202

Query: 197 IFEEVRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAINAAHVEAESAVNGIG 256
           IFEE+R+R L+VAV GIPKT+DNDI IIDRSFGF TAVE AQ+AINAAHVEA S  NGIG
Sbjct: 203 IFEEIRKRKLKVAVAGIPKTIDNDIPIIDRSFGFDTAVEEAQRAINAAHVEATSFENGIG 262

Query: 257 LVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGNGGLFPFLERRLKENGHAVLVVA 316
           LVKLMGR +G IA+HATL+SRDVDCCLIPE  F+LEG+GGLF F+++RLKE+GH V+V+A
Sbjct: 263 LVKLMGRYSGFIAMHATLASRDVDCCLIPESPFFLEGSGGLFEFIDKRLKESGHMVIVIA 322

Query: 317 EGAGQGMIPRTEAQKEE---RDESGNPVFLDVGGWLKTELKNWWATTHPGELFTVKYIDP 376
           EGAGQ ++  +E+ KE    +D SGN +  D+G W+   +K+ +A        T+KYIDP
Sbjct: 323 EGAGQDLL--SESMKESTTLKDASGNKLLQDIGLWISQRIKDHFAKK---MTLTLKYIDP 382

Query: 377 TYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIPLKEVAEAKNEVN 436
           TYMIRAVP+NA+DN  CTLLA SA+HGVMAGY GF  G +NG ++YIP   + E +N+V 
Sbjct: 383 TYMIRAVPSNASDNVCCTLLAQSAVHGVMAGYNGFTVGLVNGRHTYIPFNRITEKQNKVV 442

Query: 437 TRDHKWAWVRSVTNQPDFVK 454
             D  WA + S TNQP F+K
Sbjct: 443 ITDRMWARLLSSTNQPSFMK 454

BLAST of CsGy6G032260 vs. NCBI nr
Match: XP_004134815.1 (ATP-dependent 6-phosphofructokinase 2 [Cucumis sativus] >KGN49017.1 hypothetical protein Csa_002815 [Cucumis sativus])

HSP 1 Score: 933 bits (2411), Expect = 0.0
Identity = 454/454 (100.00%), Postives = 454/454 (100.00%), Query Frame = 0

Query: 1   MATAKISDQHPTITVADLPHLTDNFPNLKTRTNPLQTNQFFHPIDGFYATSTDIVLRRIA 60
           MATAKISDQHPTITVADLPHLTDNFPNLKTRTNPLQTNQFFHPIDGFYATSTDIVLRRIA
Sbjct: 1   MATAKISDQHPTITVADLPHLTDNFPNLKTRTNPLQTNQFFHPIDGFYATSTDIVLRRIA 60

Query: 61  FDLSGDTSPAHVAYRRAGPRRKVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYG 120
           FDLSGDTSPAHVAYRRAGPRRKVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYG
Sbjct: 61  FDLSGDTSPAHVAYRRAGPRRKVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYG 120

Query: 121 VRQIYGIVAGYRGFYSTEPVELNLKLVDNWHKRGGTALQTSRGGFDLKKIVDAIENHGFN 180
           VRQIYGIVAGYRGFYSTEPVELNLKLVDNWHKRGGTALQTSRGGFDLKKIVDAIENHGFN
Sbjct: 121 VRQIYGIVAGYRGFYSTEPVELNLKLVDNWHKRGGTALQTSRGGFDLKKIVDAIENHGFN 180

Query: 181 QVYITGGDGTMRGAVKIFEEVRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQA 240
           QVYITGGDGTMRGAVKIFEEVRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQA
Sbjct: 181 QVYITGGDGTMRGAVKIFEEVRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQA 240

Query: 241 INAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGNGGLFPF 300
           INAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGNGGLFPF
Sbjct: 241 INAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGNGGLFPF 300

Query: 301 LERRLKENGHAVLVVAEGAGQGMIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWWATT 360
           LERRLKENGHAVLVVAEGAGQGMIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWWATT
Sbjct: 301 LERRLKENGHAVLVVAEGAGQGMIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWWATT 360

Query: 361 HPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYI 420
           HPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYI
Sbjct: 361 HPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYI 420

Query: 421 PLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVKS 454
           PLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVKS
Sbjct: 421 PLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVKS 454

BLAST of CsGy6G032260 vs. NCBI nr
Match: XP_008440953.1 (PREDICTED: ATP-dependent 6-phosphofructokinase 2 [Cucumis melo])

HSP 1 Score: 906 bits (2342), Expect = 0.0
Identity = 440/454 (96.92%), Postives = 446/454 (98.24%), Query Frame = 0

Query: 1   MATAKISDQHPTITVADLPHLTDNFPNLKTRTNPLQTNQFFHPIDGFYATSTDIVLRRIA 60
           MATAKISDQHP I VADLPHLTDNFPNL+TRTNPLQTNQFFHPIDGFYATSTDIVLRRIA
Sbjct: 1   MATAKISDQHPLIAVADLPHLTDNFPNLETRTNPLQTNQFFHPIDGFYATSTDIVLRRIA 60

Query: 61  FDLSGDTSPAHVAYRRAGPRRKVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYG 120
           FDLSG+TSPAHVAYRRAGPRR VYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYG
Sbjct: 61  FDLSGNTSPAHVAYRRAGPRRTVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYG 120

Query: 121 VRQIYGIVAGYRGFYSTEPVELNLKLVDNWHKRGGTALQTSRGGFDLKKIVDAIENHGFN 180
           VR+IYGIVAGYRGFYS EPVELNLKLVDNWHKRGGTAL+TSRGGFDLKKIVDAIENHGFN
Sbjct: 121 VRRIYGIVAGYRGFYSMEPVELNLKLVDNWHKRGGTALRTSRGGFDLKKIVDAIENHGFN 180

Query: 181 QVYITGGDGTMRGAVKIFEEVRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQA 240
           QVYITGGDGTMRGAVKIFEE+RRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQA
Sbjct: 181 QVYITGGDGTMRGAVKIFEEIRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQA 240

Query: 241 INAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGNGGLFPF 300
           INAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEM FYLEGNGGLFPF
Sbjct: 241 INAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGNGGLFPF 300

Query: 301 LERRLKENGHAVLVVAEGAGQGMIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWWATT 360
           LE+RLKENGHAVLVVAEGAGQ MIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWW  T
Sbjct: 301 LEQRLKENGHAVLVVAEGAGQRMIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWWERT 360

Query: 361 HPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYI 420
           HPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYI
Sbjct: 361 HPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYI 420

Query: 421 PLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVKS 454
           PLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVKS
Sbjct: 421 PLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVKS 454

BLAST of CsGy6G032260 vs. NCBI nr
Match: KAA0025591.1 (ATP-dependent 6-phosphofructokinase 2 [Cucumis melo var. makuwa] >TYK12466.1 ATP-dependent 6-phosphofructokinase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 904 bits (2337), Expect = 0.0
Identity = 439/454 (96.70%), Postives = 445/454 (98.02%), Query Frame = 0

Query: 1   MATAKISDQHPTITVADLPHLTDNFPNLKTRTNPLQTNQFFHPIDGFYATSTDIVLRRIA 60
           MATAKISDQHP I VADLPHLTDNFPNL+TRTNPLQTNQFFHPIDGFYATSTDIVLRRIA
Sbjct: 1   MATAKISDQHPLIAVADLPHLTDNFPNLETRTNPLQTNQFFHPIDGFYATSTDIVLRRIA 60

Query: 61  FDLSGDTSPAHVAYRRAGPRRKVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYG 120
           FDLSG+TSPAHVAYRRAGPRR VYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYG
Sbjct: 61  FDLSGNTSPAHVAYRRAGPRRTVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYG 120

Query: 121 VRQIYGIVAGYRGFYSTEPVELNLKLVDNWHKRGGTALQTSRGGFDLKKIVDAIENHGFN 180
           VR+IYGIVAGYRGFYS EPVELNLKLVDNWHKRGGTAL+TSRGGFDLKKIVDAIENHGFN
Sbjct: 121 VRRIYGIVAGYRGFYSMEPVELNLKLVDNWHKRGGTALRTSRGGFDLKKIVDAIENHGFN 180

Query: 181 QVYITGGDGTMRGAVKIFEEVRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQA 240
           QVYITGGDGTMRGAVKIFEE+RRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQA
Sbjct: 181 QVYITGGDGTMRGAVKIFEEIRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQA 240

Query: 241 INAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGNGGLFPF 300
           INAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEM FYLEGNGGLFPF
Sbjct: 241 INAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGNGGLFPF 300

Query: 301 LERRLKENGHAVLVVAEGAGQGMIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWWATT 360
           LE+RLKENGHAVLVVAEGAGQ MIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWW  T
Sbjct: 301 LEQRLKENGHAVLVVAEGAGQRMIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWWERT 360

Query: 361 HPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYI 420
           HPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYI
Sbjct: 361 HPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYI 420

Query: 421 PLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVKS 454
           PLKEVAEAKNEVNTRDHKWAWVRSV NQPDFVKS
Sbjct: 421 PLKEVAEAKNEVNTRDHKWAWVRSVANQPDFVKS 454

BLAST of CsGy6G032260 vs. NCBI nr
Match: XP_038881531.1 (ATP-dependent 6-phosphofructokinase 2 isoform X1 [Benincasa hispida])

HSP 1 Score: 884 bits (2285), Expect = 0.0
Identity = 425/454 (93.61%), Postives = 443/454 (97.58%), Query Frame = 0

Query: 1   MATAKISDQHPTITVADLPHLTDNFPNLKTRTNPLQTNQFFHPIDGFYATSTDIVLRRIA 60
           MATAKISDQHP I VADLPHLTD+FP+L+TRTNPLQTNQFFHPIDGFYA+STD+VLRRIA
Sbjct: 1   MATAKISDQHPPIAVADLPHLTDDFPDLETRTNPLQTNQFFHPIDGFYASSTDVVLRRIA 60

Query: 61  FDLSGDTSPAHVAYRRAGPRRKVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYG 120
           FDLS   SPAHVAY RAGPRR VYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYG
Sbjct: 61  FDLSTKESPAHVAYHRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYG 120

Query: 121 VRQIYGIVAGYRGFYSTEPVELNLKLVDNWHKRGGTALQTSRGGFDLKKIVDAIENHGFN 180
           VR+IYGI+AGYRGFYSTEPVELNLKLVDNWHKRGGTAL+TSRGGFDL+KIVD I++HGFN
Sbjct: 121 VRRIYGILAGYRGFYSTEPVELNLKLVDNWHKRGGTALRTSRGGFDLRKIVDTIQHHGFN 180

Query: 181 QVYITGGDGTMRGAVKIFEEVRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQA 240
           QVYI GGDGTMRGAVKIF+E+RRRNLQVAVTGIPKTVDNDIGI+DRSFGFQTAVEMAQQA
Sbjct: 181 QVYIIGGDGTMRGAVKIFDEIRRRNLQVAVTGIPKTVDNDIGIVDRSFGFQTAVEMAQQA 240

Query: 241 INAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGNGGLFPF 300
           I+AAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEG+GGLFPF
Sbjct: 241 ISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGDGGLFPF 300

Query: 301 LERRLKENGHAVLVVAEGAGQGMIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWWATT 360
           LERRLKENGHAVLVVAEGAGQ MIPRTEA+KEERDESGNPVFLDVGGWLKTELKNWWA T
Sbjct: 301 LERRLKENGHAVLVVAEGAGQSMIPRTEAEKEERDESGNPVFLDVGGWLKTELKNWWART 360

Query: 361 HPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYI 420
           HPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYI
Sbjct: 361 HPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYI 420

Query: 421 PLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVKS 454
           PLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFV+S
Sbjct: 421 PLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVRS 454

BLAST of CsGy6G032260 vs. NCBI nr
Match: XP_022950529.1 (ATP-dependent 6-phosphofructokinase 2 [Cucurbita moschata])

HSP 1 Score: 854 bits (2207), Expect = 1.78e-311
Identity = 413/453 (91.17%), Postives = 435/453 (96.03%), Query Frame = 0

Query: 2   ATAKISDQHPTITVADLPHLTDNFPNLKTRTNPLQTNQFFHPIDGFYATSTDIVLRRIAF 61
           A A+I+D+HP I VA LPHLTD+FP L++RTNPLQ NQFFHPIDGFYATSTD+VLRRIAF
Sbjct: 4   AAAEIADRHPPIAVASLPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVLRRIAF 63

Query: 62  DLSGDTSPAHVAYRRAGPRRKVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGV 121
           DLS   SPAHVAY RAGPRR VYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGV
Sbjct: 64  DLSRKPSPAHVAYYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGV 123

Query: 122 RQIYGIVAGYRGFYSTEPVELNLKLVDNWHKRGGTALQTSRGGFDLKKIVDAIENHGFNQ 181
           RQI+GIVAGYRGFYS+EPVEL+LKLVD+WHKRGGTAL+TSRGGFDL++IVDAI++ GFNQ
Sbjct: 124 RQIHGIVAGYRGFYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQDRGFNQ 183

Query: 182 VYITGGDGTMRGAVKIFEEVRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAI 241
           VYI GGDGTMRGAVKIFEEV RRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAI
Sbjct: 184 VYIIGGDGTMRGAVKIFEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAI 243

Query: 242 NAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGNGGLFPFL 301
           +AAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEM FYLEG+GGLFPFL
Sbjct: 244 SAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLFPFL 303

Query: 302 ERRLKENGHAVLVVAEGAGQGMIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWWATTH 361
           ERRLKENGHAVLVVAEGAGQ  IPRTEAQKEERDESGNPVFLDVGGWLKTELK+WWA TH
Sbjct: 304 ERRLKENGHAVLVVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDWWARTH 363

Query: 362 PGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIP 421
           PGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIP
Sbjct: 364 PGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIP 423

Query: 422 LKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVKS 454
           LK+VAEAKNEVNTRDHKWAWVRSVTNQPDFV++
Sbjct: 424 LKDVAEAKNEVNTRDHKWAWVRSVTNQPDFVET 456

BLAST of CsGy6G032260 vs. ExPASy TrEMBL
Match: A0A0A0KL11 (ATP-dependent 6-phosphofructokinase OS=Cucumis sativus OX=3659 GN=PFK PE=3 SV=1)

HSP 1 Score: 933 bits (2411), Expect = 0.0
Identity = 454/454 (100.00%), Postives = 454/454 (100.00%), Query Frame = 0

Query: 1   MATAKISDQHPTITVADLPHLTDNFPNLKTRTNPLQTNQFFHPIDGFYATSTDIVLRRIA 60
           MATAKISDQHPTITVADLPHLTDNFPNLKTRTNPLQTNQFFHPIDGFYATSTDIVLRRIA
Sbjct: 1   MATAKISDQHPTITVADLPHLTDNFPNLKTRTNPLQTNQFFHPIDGFYATSTDIVLRRIA 60

Query: 61  FDLSGDTSPAHVAYRRAGPRRKVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYG 120
           FDLSGDTSPAHVAYRRAGPRRKVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYG
Sbjct: 61  FDLSGDTSPAHVAYRRAGPRRKVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYG 120

Query: 121 VRQIYGIVAGYRGFYSTEPVELNLKLVDNWHKRGGTALQTSRGGFDLKKIVDAIENHGFN 180
           VRQIYGIVAGYRGFYSTEPVELNLKLVDNWHKRGGTALQTSRGGFDLKKIVDAIENHGFN
Sbjct: 121 VRQIYGIVAGYRGFYSTEPVELNLKLVDNWHKRGGTALQTSRGGFDLKKIVDAIENHGFN 180

Query: 181 QVYITGGDGTMRGAVKIFEEVRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQA 240
           QVYITGGDGTMRGAVKIFEEVRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQA
Sbjct: 181 QVYITGGDGTMRGAVKIFEEVRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQA 240

Query: 241 INAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGNGGLFPF 300
           INAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGNGGLFPF
Sbjct: 241 INAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGNGGLFPF 300

Query: 301 LERRLKENGHAVLVVAEGAGQGMIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWWATT 360
           LERRLKENGHAVLVVAEGAGQGMIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWWATT
Sbjct: 301 LERRLKENGHAVLVVAEGAGQGMIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWWATT 360

Query: 361 HPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYI 420
           HPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYI
Sbjct: 361 HPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYI 420

Query: 421 PLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVKS 454
           PLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVKS
Sbjct: 421 PLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVKS 454

BLAST of CsGy6G032260 vs. ExPASy TrEMBL
Match: A0A1S3B325 (ATP-dependent 6-phosphofructokinase OS=Cucumis melo OX=3656 GN=LOC103485214 PE=3 SV=1)

HSP 1 Score: 906 bits (2342), Expect = 0.0
Identity = 440/454 (96.92%), Postives = 446/454 (98.24%), Query Frame = 0

Query: 1   MATAKISDQHPTITVADLPHLTDNFPNLKTRTNPLQTNQFFHPIDGFYATSTDIVLRRIA 60
           MATAKISDQHP I VADLPHLTDNFPNL+TRTNPLQTNQFFHPIDGFYATSTDIVLRRIA
Sbjct: 1   MATAKISDQHPLIAVADLPHLTDNFPNLETRTNPLQTNQFFHPIDGFYATSTDIVLRRIA 60

Query: 61  FDLSGDTSPAHVAYRRAGPRRKVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYG 120
           FDLSG+TSPAHVAYRRAGPRR VYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYG
Sbjct: 61  FDLSGNTSPAHVAYRRAGPRRTVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYG 120

Query: 121 VRQIYGIVAGYRGFYSTEPVELNLKLVDNWHKRGGTALQTSRGGFDLKKIVDAIENHGFN 180
           VR+IYGIVAGYRGFYS EPVELNLKLVDNWHKRGGTAL+TSRGGFDLKKIVDAIENHGFN
Sbjct: 121 VRRIYGIVAGYRGFYSMEPVELNLKLVDNWHKRGGTALRTSRGGFDLKKIVDAIENHGFN 180

Query: 181 QVYITGGDGTMRGAVKIFEEVRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQA 240
           QVYITGGDGTMRGAVKIFEE+RRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQA
Sbjct: 181 QVYITGGDGTMRGAVKIFEEIRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQA 240

Query: 241 INAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGNGGLFPF 300
           INAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEM FYLEGNGGLFPF
Sbjct: 241 INAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGNGGLFPF 300

Query: 301 LERRLKENGHAVLVVAEGAGQGMIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWWATT 360
           LE+RLKENGHAVLVVAEGAGQ MIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWW  T
Sbjct: 301 LEQRLKENGHAVLVVAEGAGQRMIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWWERT 360

Query: 361 HPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYI 420
           HPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYI
Sbjct: 361 HPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYI 420

Query: 421 PLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVKS 454
           PLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVKS
Sbjct: 421 PLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVKS 454

BLAST of CsGy6G032260 vs. ExPASy TrEMBL
Match: A0A5D3CPV9 (ATP-dependent 6-phosphofructokinase OS=Cucumis melo var. makuwa OX=1194695 GN=PFK PE=3 SV=1)

HSP 1 Score: 904 bits (2337), Expect = 0.0
Identity = 439/454 (96.70%), Postives = 445/454 (98.02%), Query Frame = 0

Query: 1   MATAKISDQHPTITVADLPHLTDNFPNLKTRTNPLQTNQFFHPIDGFYATSTDIVLRRIA 60
           MATAKISDQHP I VADLPHLTDNFPNL+TRTNPLQTNQFFHPIDGFYATSTDIVLRRIA
Sbjct: 1   MATAKISDQHPLIAVADLPHLTDNFPNLETRTNPLQTNQFFHPIDGFYATSTDIVLRRIA 60

Query: 61  FDLSGDTSPAHVAYRRAGPRRKVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYG 120
           FDLSG+TSPAHVAYRRAGPRR VYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYG
Sbjct: 61  FDLSGNTSPAHVAYRRAGPRRTVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYG 120

Query: 121 VRQIYGIVAGYRGFYSTEPVELNLKLVDNWHKRGGTALQTSRGGFDLKKIVDAIENHGFN 180
           VR+IYGIVAGYRGFYS EPVELNLKLVDNWHKRGGTAL+TSRGGFDLKKIVDAIENHGFN
Sbjct: 121 VRRIYGIVAGYRGFYSMEPVELNLKLVDNWHKRGGTALRTSRGGFDLKKIVDAIENHGFN 180

Query: 181 QVYITGGDGTMRGAVKIFEEVRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQA 240
           QVYITGGDGTMRGAVKIFEE+RRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQA
Sbjct: 181 QVYITGGDGTMRGAVKIFEEIRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQA 240

Query: 241 INAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGNGGLFPF 300
           INAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEM FYLEGNGGLFPF
Sbjct: 241 INAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGNGGLFPF 300

Query: 301 LERRLKENGHAVLVVAEGAGQGMIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWWATT 360
           LE+RLKENGHAVLVVAEGAGQ MIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWW  T
Sbjct: 301 LEQRLKENGHAVLVVAEGAGQRMIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWWERT 360

Query: 361 HPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYI 420
           HPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYI
Sbjct: 361 HPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYI 420

Query: 421 PLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVKS 454
           PLKEVAEAKNEVNTRDHKWAWVRSV NQPDFVKS
Sbjct: 421 PLKEVAEAKNEVNTRDHKWAWVRSVANQPDFVKS 454

BLAST of CsGy6G032260 vs. ExPASy TrEMBL
Match: A0A6J1GG28 (ATP-dependent 6-phosphofructokinase OS=Cucurbita moschata OX=3662 GN=LOC111453612 PE=3 SV=1)

HSP 1 Score: 854 bits (2207), Expect = 8.62e-312
Identity = 413/453 (91.17%), Postives = 435/453 (96.03%), Query Frame = 0

Query: 2   ATAKISDQHPTITVADLPHLTDNFPNLKTRTNPLQTNQFFHPIDGFYATSTDIVLRRIAF 61
           A A+I+D+HP I VA LPHLTD+FP L++RTNPLQ NQFFHPIDGFYATSTD+VLRRIAF
Sbjct: 4   AAAEIADRHPPIAVASLPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVLRRIAF 63

Query: 62  DLSGDTSPAHVAYRRAGPRRKVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGV 121
           DLS   SPAHVAY RAGPRR VYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGV
Sbjct: 64  DLSRKPSPAHVAYYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGV 123

Query: 122 RQIYGIVAGYRGFYSTEPVELNLKLVDNWHKRGGTALQTSRGGFDLKKIVDAIENHGFNQ 181
           RQI+GIVAGYRGFYS+EPVEL+LKLVD+WHKRGGTAL+TSRGGFDL++IVDAI++ GFNQ
Sbjct: 124 RQIHGIVAGYRGFYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQDRGFNQ 183

Query: 182 VYITGGDGTMRGAVKIFEEVRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAI 241
           VYI GGDGTMRGAVKIFEEV RRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAI
Sbjct: 184 VYIIGGDGTMRGAVKIFEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAI 243

Query: 242 NAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGNGGLFPFL 301
           +AAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEM FYLEG+GGLFPFL
Sbjct: 244 SAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLFPFL 303

Query: 302 ERRLKENGHAVLVVAEGAGQGMIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWWATTH 361
           ERRLKENGHAVLVVAEGAGQ  IPRTEAQKEERDESGNPVFLDVGGWLKTELK+WWA TH
Sbjct: 304 ERRLKENGHAVLVVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDWWARTH 363

Query: 362 PGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIP 421
           PGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIP
Sbjct: 364 PGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIP 423

Query: 422 LKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVKS 454
           LK+VAEAKNEVNTRDHKWAWVRSVTNQPDFV++
Sbjct: 424 LKDVAEAKNEVNTRDHKWAWVRSVTNQPDFVET 456

BLAST of CsGy6G032260 vs. ExPASy TrEMBL
Match: A0A6J1ISR0 (ATP-dependent 6-phosphofructokinase OS=Cucurbita maxima OX=3661 GN=LOC111478077 PE=3 SV=1)

HSP 1 Score: 852 bits (2201), Expect = 7.63e-311
Identity = 412/453 (90.95%), Postives = 434/453 (95.81%), Query Frame = 0

Query: 2   ATAKISDQHPTITVADLPHLTDNFPNLKTRTNPLQTNQFFHPIDGFYATSTDIVLRRIAF 61
           A A+ISD+HP I VA LPHLTD+FP L++RTNPLQ NQFFHPIDGFYATSTD+VLRRIAF
Sbjct: 6   AAAEISDRHPPIAVASLPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVLRRIAF 65

Query: 62  DLSGDTSPAHVAYRRAGPRRKVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGV 121
           DLS   SPAHVAY RAGPRR VYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGV
Sbjct: 66  DLSRKPSPAHVAYYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGV 125

Query: 122 RQIYGIVAGYRGFYSTEPVELNLKLVDNWHKRGGTALQTSRGGFDLKKIVDAIENHGFNQ 181
           RQI+GIVAGYRGFYS+EPVEL+LKLVD+WHKRGGTAL+TSRGGFDL++IVDAI++ GFNQ
Sbjct: 126 RQIHGIVAGYRGFYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQDRGFNQ 185

Query: 182 VYITGGDGTMRGAVKIFEEVRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAI 241
           VYI GGDGTMRGAVKIFEEV RR LQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAI
Sbjct: 186 VYIIGGDGTMRGAVKIFEEVCRRELQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAI 245

Query: 242 NAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGNGGLFPFL 301
           +AAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEM FYLEG+GGLFPFL
Sbjct: 246 SAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLFPFL 305

Query: 302 ERRLKENGHAVLVVAEGAGQGMIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWWATTH 361
           ERRLKENGHAVLVVAEGAGQ  IPRTEAQKEERDESGNPVFLDVGGWLKTELK+WWA TH
Sbjct: 306 ERRLKENGHAVLVVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDWWARTH 365

Query: 362 PGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIP 421
           PGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIP
Sbjct: 366 PGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIP 425

Query: 422 LKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVKS 454
           LK+VAEAKNEVNT+DHKWAWVRSVTNQPDFV++
Sbjct: 426 LKDVAEAKNEVNTKDHKWAWVRSVTNQPDFVET 458

BLAST of CsGy6G032260 vs. TAIR 10
Match: AT5G47810.1 (phosphofructokinase 2 )

HSP 1 Score: 697.2 bits (1798), Expect = 8.7e-201
Identity = 334/438 (76.26%), Postives = 378/438 (86.30%), Query Frame = 0

Query: 14  TVADLPHLTDNFPNLKTRTNPLQTNQFFHPIDGFYATSTDIVLRRIAFDLSGDTSPAHVA 73
           ++  LP L+     L+ R NPL+ N +FHP +GFY T +D++L ++A+D S   S + VA
Sbjct: 6   SIRKLPSLS----GLRHRRNPLEDNPYFHPSNGFYITPSDVILAQVAYDHSAH-SQSRVA 65

Query: 74  YRRAGPRRKVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGVRQIYGIVAGYRG 133
           Y RAGPRR++ +EP +V+AAIVTCGGLCPGMNTVIRELVVGLWELYGVR+IYGI AGYRG
Sbjct: 66  YHRAGPRREIMYEPSAVKAAIVTCGGLCPGMNTVIRELVVGLWELYGVREIYGIPAGYRG 125

Query: 134 FYSTEPVELNLKLVDNWHKRGGTALQTSRGGFDLKKIVDAIENHGFNQVYITGGDGTMRG 193
           FYS + V+L+ K V +WHK+GGT L TSRGGF L+KIVDAI  +G+NQVYI GGDGTMRG
Sbjct: 126 FYSMKAVKLDPKAVHDWHKKGGTVLATSRGGFHLQKIVDAIHLNGYNQVYIIGGDGTMRG 185

Query: 194 AVKIFEEVRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAINAAHVEAESAVN 253
           AV+IF+E+  R L+V +T IPKTVDND+GIIDRSFGFQTAVEMAQ+AI+AAHVEAESAVN
Sbjct: 186 AVEIFKEISLRKLEVGITVIPKTVDNDVGIIDRSFGFQTAVEMAQEAISAAHVEAESAVN 245

Query: 254 GIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGNGGLFPFLERRLKENGHAVL 313
           GIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEM FYLEG GGLF FLE+RLKE GHAVL
Sbjct: 246 GIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMDFYLEGKGGLFEFLEKRLKERGHAVL 305

Query: 314 VVAEGAGQGMIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWWATTHPGELFTVKYIDP 373
           VVAEGAGQ MIPR E+QK+ERDESGN VFLDVG W K+ LK WW   HP ELFTVKYIDP
Sbjct: 306 VVAEGAGQEMIPRNESQKQERDESGNAVFLDVGVWFKSVLKAWWEREHPDELFTVKYIDP 365

Query: 374 TYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIPLKEVAEAKNEVN 433
           TYMIRAVPANATDN YCTLLAHSAIHGVMAGYTGFV GPINGNY+YIPL+EVA+ KN+VN
Sbjct: 366 TYMIRAVPANATDNLYCTLLAHSAIHGVMAGYTGFVPGPINGNYAYIPLEEVAQTKNQVN 425

Query: 434 TRDHKWAWVRSVTNQPDF 452
           TRDHKWAWVRSVTNQPDF
Sbjct: 426 TRDHKWAWVRSVTNQPDF 438

BLAST of CsGy6G032260 vs. TAIR 10
Match: AT4G26270.1 (phosphofructokinase 3 )

HSP 1 Score: 519.2 bits (1336), Expect = 3.3e-147
Identity = 254/436 (58.26%), Postives = 318/436 (72.94%), Query Frame = 0

Query: 17  DLPHLTDNFPNLKTRTNPLQTNQFFHPIDGFYATSTDIVLRRIAFDLSGDTSPAHVAYRR 76
           D+PHL+D  P L T  NPLQ N  +  +  ++  + D V ++I     G   P  + +RR
Sbjct: 22  DVPHLSDYLPGLPTYPNPLQDNPAYSVVKQYFVDADDSVPQKIVVHKDG---PRGIHFRR 81

Query: 77  AGPRRKVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGVRQIYGIVAGYRGFYS 136
           AGPR+KVYFE + V A IVTCGGLCPG+NTVIRE+V  L  +YGV++I GI  GYRGFY+
Sbjct: 82  AGPRQKVYFESDEVHACIVTCGGLCPGLNTVIREIVSSLSYMYGVKRILGIDGGYRGFYA 141

Query: 137 TEPVELNLKLVDNWHKRGGTALQTSRGGFDLKKIVDAIENHGFNQVYITGGDGTMRGAVK 196
              V L+ K+V++ HKRGGT L TSRGG D  KIVD+I++ G NQVYI GGDGT RGA  
Sbjct: 142 KNTVSLDSKVVNDIHKRGGTILGTSRGGHDTTKIVDSIQDRGINQVYIIGGDGTQRGASV 201

Query: 197 IFEEVRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAINAAHVEAESAVNGIG 256
           IFEE+RRR L+VAV GIPKT+DNDI +ID+SFGF TAVE AQ+AINAAHVEAES  NGIG
Sbjct: 202 IFEEIRRRGLKVAVIGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHVEAESIENGIG 261

Query: 257 LVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGNGGLFPFLERRLKENGHAVLVVA 316
           +VKLMGR +G IA++ATL+SRDVDCCLIPE  FYLEG GGLF ++E+RLKE+GH VLV+A
Sbjct: 262 VVKLMGRYSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFEYIEKRLKESGHMVLVIA 321

Query: 317 EGAGQGMIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWWATTHPGELFTVKYIDPTYM 376
           EGAGQ ++ ++      +D SGN +  DVG WL   +K+ +       +  +KYIDPTYM
Sbjct: 322 EGAGQDLMSKSMESMTLKDASGNKLLKDVGLWLSQSIKDHF--NQKKMVMNLKYIDPTYM 381

Query: 377 IRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIPLKEVAEAKNEVNTRD 436
           IRAVP+NA+DN YCTLLA SA+HG MAGYTG++SG +NG  +YIP   + E +N V   D
Sbjct: 382 IRAVPSNASDNVYCTLLAQSAVHGAMAGYTGYISGLVNGRQTYIPFYRITEKQNHVVITD 441

Query: 437 HKWAWVRSVTNQPDFV 453
             WA + S TNQP F+
Sbjct: 442 RMWARLLSSTNQPSFL 452

BLAST of CsGy6G032260 vs. TAIR 10
Match: AT5G56630.1 (phosphofructokinase 7 )

HSP 1 Score: 514.6 bits (1324), Expect = 8.0e-146
Identity = 253/436 (58.03%), Postives = 316/436 (72.48%), Query Frame = 0

Query: 17  DLPHLTDNFPNLKTRTNPLQTNQFFHPIDGFYATSTDIVLRRIAFDLSGDTSPAHVAYRR 76
           D+PHL D  P+L T  NPLQ N  +  +  ++  + D V  ++     G   P  V +RR
Sbjct: 22  DVPHLIDYLPDLPTYPNPLQDNPAYSVVKQYFVHADDSVPEKVVVHKDG---PRGVHFRR 81

Query: 77  AGPRRKVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGVRQIYGIVAGYRGFYS 136
           AGPR+KVYFE + V A IVTCGGLCPG+NTVIRE+V  L  +YGV++I GI  GYRGFY+
Sbjct: 82  AGPRQKVYFESDEVHACIVTCGGLCPGLNTVIREVVSSLSYMYGVKRILGIDGGYRGFYA 141

Query: 137 TEPVELNLKLVDNWHKRGGTALQTSRGGFDLKKIVDAIENHGFNQVYITGGDGTMRGAVK 196
              + LN K+V++ HKRGGT + TSRGG D  KIVD+I++ G NQVYI GGDGT RGA  
Sbjct: 142 KNTIPLNSKVVNDIHKRGGTIIGTSRGGHDTNKIVDSIQDRGINQVYIIGGDGTQRGASV 201

Query: 197 IFEEVRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAINAAHVEAESAVNGIG 256
           IFEE+RRR L+VAV GIPKT+DNDI +ID+SFGF TAVE AQ+AINAAHVEAES  NGIG
Sbjct: 202 IFEEIRRRRLKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHVEAESNENGIG 261

Query: 257 LVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGNGGLFPFLERRLKENGHAVLVVA 316
            VKLMGR +G+IA++ATL+SRDVDCCLIPE  FYLEG GGLF F+ERRLK++GH V+V+A
Sbjct: 262 FVKLMGRYSGYIAMYATLASRDVDCCLIPESPFYLEGEGGLFEFIERRLKDHGHMVIVLA 321

Query: 317 EGAGQGMIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWWATTHPGELFTVKYIDPTYM 376
           EGAGQ ++ ++  +    D SGN +  DVG WL   +K+ +       +  +KYIDPTYM
Sbjct: 322 EGAGQDLMCKS-MESTPMDASGNKLLKDVGLWLSQSIKDHFKKNK--MVMNLKYIDPTYM 381

Query: 377 IRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIPLKEVAEAKNEVNTRD 436
           IRAVP+NA+DN YCTLLA SA+HG MAGYTG+ SG +NG  +YIP   + E +N V   D
Sbjct: 382 IRAVPSNASDNVYCTLLAQSAVHGAMAGYTGYTSGLVNGRQTYIPFYRITETQNNVVITD 441

Query: 437 HKWAWVRSVTNQPDFV 453
             WA + S TNQP F+
Sbjct: 442 RMWARLLSSTNQPSFL 451

BLAST of CsGy6G032260 vs. TAIR 10
Match: AT5G61580.1 (phosphofructokinase 4 )

HSP 1 Score: 511.9 bits (1317), Expect = 5.2e-145
Identity = 248/436 (56.88%), Postives = 313/436 (71.79%), Query Frame = 0

Query: 17  DLPHLTDNFPNLKTRTNPLQTNQFFHPIDGFYATSTDIVLRRIAFDLSGDTSPAHVAYRR 76
           D+PHLT   P+L +  NPL+ +Q +  +   + +S D+V + I        S   V +RR
Sbjct: 73  DVPHLTKFLPDLPSYPNPLKESQAYAIVKRTFVSSEDVVAQNIVVQKG---SKRGVHFRR 132

Query: 77  AGPRRKVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGVRQIYGIVAGYRGFYS 136
           AGPR +VYF  + V+A IVTCGGLCPG+NTVIRE+V GL  +YGV  I GI  GYRGFYS
Sbjct: 133 AGPRERVYFRSDEVKACIVTCGGLCPGINTVIREIVCGLNNMYGVNNILGIQGGYRGFYS 192

Query: 137 TEPVELNLKLVDNWHKRGGTALQTSRGGFDLKKIVDAIENHGFNQVYITGGDGTMRGAVK 196
              + L  K+V++ HKRGGT LQTSRGG D  KIVD I++ G NQVYI GG GT +GA K
Sbjct: 193 KNTMNLTPKVVNDIHKRGGTFLQTSRGGHDTAKIVDNIQDRGINQVYIIGGGGTQKGAEK 252

Query: 197 IFEEVRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAINAAHVEAESAVNGIG 256
           I+EEV RR LQVAV+GIPKT+DNDI +ID+SFGF TAVE AQ+AINAAHVE ES  NG+G
Sbjct: 253 IYEEVERRGLQVAVSGIPKTIDNDIAVIDKSFGFDTAVEEAQRAINAAHVEVESVENGVG 312

Query: 257 LVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGNGGLFPFLERRLKENGHAVLVVA 316
           +VKLMGR +G IA+ ATL++RDVDCCLIPE  F+LEG GGLF F+E RLKEN H V+V+A
Sbjct: 313 IVKLMGRYSGFIAMIATLANRDVDCCLIPESPFFLEGKGGLFEFIEERLKENRHMVIVIA 372

Query: 317 EGAGQGMIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWWATTHPGELFTVKYIDPTYM 376
           EGAGQ  + ++    E +D SGN + LDVG WL  ++K+ +       +  +KYIDPTYM
Sbjct: 373 EGAGQDYVAQSMRASETKDASGNRLLLDVGLWLTQQIKDHFTNVRK-MMINMKYIDPTYM 432

Query: 377 IRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIPLKEVAEAKNEVNTRD 436
           IRA+P+NA+DN YCTLLA SA+HG MAGY+GF  GP+N  ++YIP+ +V E  N V   D
Sbjct: 433 IRAIPSNASDNVYCTLLAQSAVHGAMAGYSGFTVGPVNSRHAYIPISQVTEVTNTVKLTD 492

Query: 437 HKWAWVRSVTNQPDFV 453
             WA + + TNQP F+
Sbjct: 493 RMWARLLASTNQPSFL 504

BLAST of CsGy6G032260 vs. TAIR 10
Match: AT4G29220.1 (phosphofructokinase 1 )

HSP 1 Score: 510.0 bits (1312), Expect = 2.0e-144
Identity = 251/440 (57.05%), Postives = 321/440 (72.95%), Query Frame = 0

Query: 17  DLPHLTDNFPNLKTRTNPLQTNQFFHPIDGFYATSTDIVLRRIAFDLSGDTSPAHVAYRR 76
           D+PH +D+FP+  T  NPLQ N  +  +  ++    D V ++I        SP    +RR
Sbjct: 23  DVPHFSDDFPDHPTYPNPLQDNAAYSVVKQYFVDEDDTVPQKIVVH---PDSPRGTHFRR 82

Query: 77  AGPRRKVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGVRQIYGIVAGYRGFYS 136
           AGPR++VYFE + V A IVTCGGLCPG+NTVIRE+V GL  +YGV++I GI  GYRGFY+
Sbjct: 83  AGPRQRVYFESDDVLACIVTCGGLCPGLNTVIREIVCGLSYMYGVKRILGIDGGYRGFYA 142

Query: 137 TEPVELNLKLVDNWHKRGGTALQTSRGGFDLKKIVDAIENHGFNQVYITGGDGTMRGAVK 196
              + L+LK V++ H+ GGT L TSRGG +  KIVD+I++ G NQVYI GGDG+ +GA  
Sbjct: 143 RNTIHLDLKTVNDIHRSGGTILGTSRGGHNTTKIVDSIQDRGINQVYIIGGDGSQKGAAA 202

Query: 197 IFEEVRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAINAAHVEAESAVNGIG 256
           IFEE+R+R L+VAV GIPKT+DNDI IIDRSFGF TAVE AQ+AINAAHVEA S  NGIG
Sbjct: 203 IFEEIRKRKLKVAVAGIPKTIDNDIPIIDRSFGFDTAVEEAQRAINAAHVEATSFENGIG 262

Query: 257 LVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGNGGLFPFLERRLKENGHAVLVVA 316
           LVKLMGR +G IA+HATL+SRDVDCCLIPE  F+LEG+GGLF F+++RLKE+GH V+V+A
Sbjct: 263 LVKLMGRYSGFIAMHATLASRDVDCCLIPESPFFLEGSGGLFEFIDKRLKESGHMVIVIA 322

Query: 317 EGAGQGMIPRTEAQKEE---RDESGNPVFLDVGGWLKTELKNWWATTHPGELFTVKYIDP 376
           EGAGQ ++  +E+ KE    +D SGN +  D+G W+   +K+ +A        T+KYIDP
Sbjct: 323 EGAGQDLL--SESMKESTTLKDASGNKLLQDIGLWISQRIKDHFAKK---MTLTLKYIDP 382

Query: 377 TYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIPLKEVAEAKNEVN 436
           TYMIRAVP+NA+DN  CTLLA SA+HGVMAGY GF  G +NG ++YIP   + E +N+V 
Sbjct: 383 TYMIRAVPSNASDNVCCTLLAQSAVHGVMAGYNGFTVGLVNGRHTYIPFNRITEKQNKVV 442

Query: 437 TRDHKWAWVRSVTNQPDFVK 454
             D  WA + S TNQP F+K
Sbjct: 443 ITDRMWARLLSSTNQPSFMK 454

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FIK01.2e-19976.26ATP-dependent 6-phosphofructokinase 2 OS=Arabidopsis thaliana OX=3702 GN=PFK2 PE... [more]
Q94AA44.6e-14658.26ATP-dependent 6-phosphofructokinase 3 OS=Arabidopsis thaliana OX=3702 GN=PFK3 PE... [more]
Q9C5J71.1e-14458.03ATP-dependent 6-phosphofructokinase 7 OS=Arabidopsis thaliana OX=3702 GN=PFK7 PE... [more]
Q9FKG37.3e-14456.88ATP-dependent 6-phosphofructokinase 4, chloroplastic OS=Arabidopsis thaliana OX=... [more]
Q9M0F92.8e-14357.05ATP-dependent 6-phosphofructokinase 1 OS=Arabidopsis thaliana OX=3702 GN=PFK1 PE... [more]
Match NameE-valueIdentityDescription
XP_004134815.10.0100.00ATP-dependent 6-phosphofructokinase 2 [Cucumis sativus] >KGN49017.1 hypothetical... [more]
XP_008440953.10.096.92PREDICTED: ATP-dependent 6-phosphofructokinase 2 [Cucumis melo][more]
KAA0025591.10.096.70ATP-dependent 6-phosphofructokinase 2 [Cucumis melo var. makuwa] >TYK12466.1 ATP... [more]
XP_038881531.10.093.61ATP-dependent 6-phosphofructokinase 2 isoform X1 [Benincasa hispida][more]
XP_022950529.11.78e-31191.17ATP-dependent 6-phosphofructokinase 2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A0A0KL110.0100.00ATP-dependent 6-phosphofructokinase OS=Cucumis sativus OX=3659 GN=PFK PE=3 SV=1[more]
A0A1S3B3250.096.92ATP-dependent 6-phosphofructokinase OS=Cucumis melo OX=3656 GN=LOC103485214 PE=3... [more]
A0A5D3CPV90.096.70ATP-dependent 6-phosphofructokinase OS=Cucumis melo var. makuwa OX=1194695 GN=PF... [more]
A0A6J1GG288.62e-31291.17ATP-dependent 6-phosphofructokinase OS=Cucurbita moschata OX=3662 GN=LOC11145361... [more]
A0A6J1ISR07.63e-31190.95ATP-dependent 6-phosphofructokinase OS=Cucurbita maxima OX=3661 GN=LOC111478077 ... [more]
Match NameE-valueIdentityDescription
AT5G47810.18.7e-20176.26phosphofructokinase 2 [more]
AT4G26270.13.3e-14758.26phosphofructokinase 3 [more]
AT5G56630.18.0e-14658.03phosphofructokinase 7 [more]
AT5G61580.15.2e-14556.88phosphofructokinase 4 [more]
AT4G29220.12.0e-14457.05phosphofructokinase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR022953ATP-dependent 6-phosphofructokinasePRINTSPR00476PHFRCTKINASEcoord: 211..228
score: 36.18
coord: 94..113
score: 45.13
coord: 307..319
score: 37.28
coord: 229..247
score: 26.32
coord: 250..266
score: 28.51
coord: 178..194
score: 39.06
IPR012004Pyrophosphate-dependent phosphofructokinase TP0108-typePIRSFPIRSF000534ATP_PFK_TP0108coord: 12..454
e-value: 3.0E-174
score: 576.9
IPR012004Pyrophosphate-dependent phosphofructokinase TP0108-typeHAMAPMF_01981Phosphofructokinase_II_Xcoord: 18..452
score: 51.729248
IPR000023Phosphofructokinase domainPFAMPF00365PFKcoord: 92..399
e-value: 1.8E-56
score: 191.6
NoneNo IPR availableGENE3D3.40.50.450coord: 86..275
e-value: 1.5E-57
score: 196.0
NoneNo IPR availablePANTHERPTHR45770FAMILY NOT NAMEDcoord: 11..454
NoneNo IPR availablePANTHERPTHR45770:SF9ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASE 2coord: 11..454
IPR035966Phosphofructokinase superfamilySUPERFAMILY53784Phosphofructokinasecoord: 26..451

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy6G032260.1CsGy6G032260.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006002 fructose 6-phosphate metabolic process
biological_process GO:0061615 glycolytic process through fructose-6-phosphate
biological_process GO:0006096 glycolytic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0003872 6-phosphofructokinase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0046872 metal ion binding