CsGy6G029090 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy6G029090
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionSerine hydroxymethyltransferase
LocationGy14Chr6: 27820382 .. 27825379 (-)
RNA-Seq ExpressionCsGy6G029090
SyntenyCsGy6G029090
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGAGCTTATTCATCAAAAGCACTAAAATACACATATTATTAATATATGCATTTTTTCTATTTACAAAATAAAATGTGGGAGTGTTGAGTGTGAGAGTGGATAAAACGAAGCAGATAGAGAGAATATGGCACTGGCAATGGCCATGGCGCTACGTAGGCTTTCTTCTTCCATTCACAGCCCCCTTCGTCACCTCCATGGAGCCTCTTTCCGTTATCTGGTCTCTTTCTCTTTTCTATTATATTCTTCTCTTTACTACTTCTTTTTCTTTTTATTTTGCTCTCCGCAATAACGCATGCCTGAGCTGCTCCGAGCTCATTAACGAACCCCGCTTAATTACCATGCGTTCTTCTTCCTTTTTCTTCTTACTCAATCAAGAAACACATAAGAGATTGTCTAGATTGATTACTCTATAATTCTTACCCTCCATGTATGTTCTAATCCTCAACTGTTTCATTGTTCTCTTAGTCTTCTTTGCCCAACGAAGCTGTCTATGATAAGGAGAGACCGCGCGTTCCGGTAATTCAATCTCTCTCTTCTTCTTTGATTCTGTATACTCGCATTTCATGTCTCAAATTATGCTTATGGTTGCATATCTCTTACAGTGGCCGAAACAACTGAACGATCCGCTCGAGGTAATTGATCCAGAAATTGCGGACATAATCGAACTCGAGAAGGCCAGGCAATGGAAGGTGAGCAGTTTTGCTGTGAAGTGTGTTGTGCTTTTTAGTTGGTTATTTTAATTGCTTGAATAATTTGGATGCTTATTTGATCAATCAACATCGTTCATTTAAAATTGGTTTGGCTGGGATGAGATAAAAACCTGTGGTACTCAACAACTCAATCCATTTGAAGTGTTTTTTTTATTCTTTCAGTTCTCATGTTTTTTCAGTTGACAACTTATTTATATTTACGCTCTTGTTAATTTGAGTTCTTGTGTGATAGTTAACCACGCTCCATAATAGATGGTATTTGCATATATTGTTGAGTCAAGTGCTTAATTTCTCTTTGAAGTTCAATCTTCCAATGGAAACCGTAATCTATTTGTTATTTGAAGGGCCTGGAACTCATACCATCTGAGAACTTCACTTCCGTCTCTGTGATGCAAGCAGTTGGATCTGTTATGACTAACAAATATAGTGAAGGTTATCCTGGGGCTAGATACTATGGAGGAAACGAGTACGTGATTCTTTCGGTTTTTTCTTAAAATTTCATTTTTCTTGTTATTTGATGGCCATACCTTAAAGTAGCCAATAAATATGTAAATAATTCCTTAACTCCTATTGTTTATTGGCTGTGCAACACTGAAGGTTTCGTTTTTTCATTATCATCGCCATCATTTTTCTTTTGAAAATTGAATCCTCATTATCATCGTTATTTTTCTACATTTTGTAGGTACATCGACATGGCCGAATCCTTGTGTCAGAAACGTGCTCTGGAAGCATTTCGATTGGATCCAGAAAAATGGGGAGGTATTAAATATAATTGTCTGGAAATAACGAATGTCTAGAATCACTATATGGTATATAAATTTTATCCAGTGGGATACATACAGCCATTGGTTAATGGAGAAAGGTTCACATATGCATTGGTATAGGAATGTTTTAACTGGGGAACTTTCAGTCATTCACAATTTTTTTATTGAATATCATGGTTTTGTTGCAGTGAATGTTCAATCTTTATCTGGCTCTCCATCCAATTTTCAAGTTTATACTGCATTGCTAAAACCCCACGAAAGAATTATGGCGCTTGATCTTCCTCATGGTGGACATCTCTCTCATGGTTACCAGGTGCCTGGCTGTAACTTTAAGAACTCTGTTGATATAAACCTCTATATCTTTCATTTTTTTTATTATTTTTAATTATCATCATGCTCTAGTTCTGTAATATACGTTTCTCATCAATCTCTCGATGTTACTCAACCGGTTTCTTTTTAGCCATTCATTTTTTTAAAAAAATTGTTTTTTAGCGATTGCTATTTTATGAACAAATATCAATAATATGTTATTAACATTATTCCTCTGGCTATTTTACTATAAGAAGTATCCTTTTTAGTAAATCATTTTTTGGTCCCTGCTTAACTAACAAGTAACTAAAAAAAGACGTCTCTTGTGTGATTGTGCAGACTGATACCAAAAAGATCTCTGCAGTATCTATATTTTTTGAGACAATGCCATACAGATTGGATGAGAGCACGGGCTATATTGACTATGATCAGGTATTGTGCAATTTATTCTCACCATTCTTTTATCTTTGTTATATAGTCCCAAGCAAATTCATAAGGTAGAAAATTTTGAGGAGTCCATTTCAAGCAAACAATATGGTGATCTGGAAATACTAACAAGTTTCTAGCATTTTTTTTTGCCAAAAAAACCGGTTTTCTGACTAACTCTTCTTCCAGATCAGAGTTTTATCCAAGTCTTCACTATATCAAGTATCTTTGAATTTTTGACCAGAGTTTATTAATAGTCTACGCAGTATATGAAAGTGTAGTTTTGAACTTACTGGATTAGTGATATAGTTCCTTTTGGTGGAAATTGAGCCATTAATAAACTTTGAAGTCATGTTACTGATGCATTTAACCACAAATGACAGTTGGAGAGAAGTGCTACTCTTTTCCGGCCTAAGTTAATTGTTGCTGGTGCCAGTGCCTATGCACGCTTATATGATTATGCACGTATACGCAAGGTACACTCATTGACTTTCATATGCATTTCAACGTGAGCACTTCAATTCTATCAAAAATTGTGGTCTCGATCTCAGGCTACACCTTGTATGGTCCAAGGCTCACATGTGCTAAGCCTTTCCACAGGTCTGTGATAAACAAAAAGCTATAATGTTGGCAGATATGGCACATATCAGTGGATTGGTTGCAGCTGATGTTATCCCTTCACCATTTGAATATGCTGATATTGTAACCACCACGACTCACAAGTCACTTCGTGGCCCACGTGGAGCCATGATATTTTTTAGGAAAGGAGTGAAAGAGATAAATAAACAAGGGCGGGAGGTACTTCTGTTTGGTTTTTCATAAGTGCAAATATTATCCTTGACCTTCTGTTTTCTGTCATTATTCATTATTCTCACTTTAGGATAGATGCATTTTTTAAAAAAATTTCTGAATTGCAAATTTAAGGCAATTATATGAAGTTGTAATAATGTCAACATCTCCTGCATCCCCCTGAAGGTGTTATATGACTACGAGGACAAAATCAATCAGGCTGTCTTTCCTGGTCTACAAGGTGGTCCGCACAACCACACAATTACTGGTTTAGCAGTTGCACTGAAACAGGTTGGCTTGATGATAAACATGTTTCTCTTATTGATTTTACTAGGAATATGAAAATTTTGCAAAAAAATTTCATTTATGGATGTAGGCAACAACTCCAGAATACAAAGCCTATCAAGAGCAAGTTCTTAGGAATTGCTCAAATTTTGCACAGGTATCTAATTTTTATTAGATGAACTAAATTGTCTCCCAGGACATTTTCATTATCAGGTACAAAAGTTTTATTTGACTTGCTTGTGATATTTTGGCTGACCATATTAGAAAAACTAATGCAGTCTCTCGCTGAGAAAGGCTATGAACTTGTATCTGGTGGAACTGATAATCATCTGGTTCTAGTAAATTTGAAGAATAAGGTAAGAGAGCACCTTGCTTTTGTTTAGCTATGTAAAGAAGCTGTTTTTATTTTTTTCTTATTCGTAGTTCTTTGTGAATTTAAATGCTTAGAGAAACTAATAATCTTCTGTTTAGTTTGTTGCCCTTAACAAGATATTTTCTAAAGGTTACGTTGTTATGTATATTCTGGTTATGAAGTTTCGCTCAAGAAGACACTCTTAGGAAAAAAAGTGAAAACTTGACTATTATTTTAAATAAAACCGTCCAGGGTATTGATGGTTCAAGAGTTGAAAAGGTGCTGGAATCAGTTCATATCGCTGCCAACAAAAACACTGTTCCTGGAGATGTCTCAGCTATGGTTCCCGGTGGTATCAGGATGGGTAAAATCCAAAATTGAGCTGATATCCATTGTGGTTACCATCAGTTTAGCTAATATCCATTTATCATTCAGGAACTCCCGCTCTTACCTCCAGAGGATTTGTTGAGGAAGACTTCGCTAAGGTAGCAGAGTTCTTTGATGCTGCTGTGAACATAGCCGTAAAGATCAAAGCAGAAACCAAAGGTTCCTAATGCCCTTCTAATTCAACTTTACGTGTACTTGGCTTTTCATTTATTTTTGCCAAATCTGGGTCTGTCCCTTCTAAGATAAGATTTCTCATCTTGTTTATCCAGGAACAAAATTGAAGGACTTTCTTACCACAATGGAATCAACTCCTTACTTCCAGTCCGAGATCAAAAATCTAAAACAGGATGTTGAGGAATATGCAAAGAAATTTCCTACTATTGGTTTTGAAAAGGAAACAATGAAATACAAGAGCTGAGAGAAAAAGGCCTGCCTGGTAAGAGATCATTTTAGAAAAAAAAAAAATTGTCATGTTATATAAATAACTAAACTGGATTATCGTTATCTTCCTTGTTGCAAGAAGACTCTAAGATTTTACCAGATATATAAATATATATTAAAAAGAACTACTTTTTATTTTGTTTTAAAGCTGGGTTTCTAATATTACTTTTCAAACTTGGAAACTTATAGGCAAAATGTCGATCACTTTCTTCTAAATAACATAAAGTTAAATCCGTATGCTCTAGCTGTCTCAGTTCTGAAATGTTTATATGTCTGAAACAGGGATGAAGCAGAAAAGAAATGGTGACGAAGAAACTAAAGGGTCATGTGTCAAGTTTGTAAGTTTTAATTATTTGAAATGCACAAATTCAGCAGATCATTTTGATTGGGTCTGAGATGTATATATTGCATTTTGAAGTTATCTACGAAAATAAACTGGTTTGATTCCAGTTATTTCTTTCCCACTAGTTTGTCATCATTTGATCTATCTTTTTTTCTTTTATTAATCCTATTAACTTTGTTATTATGACCTTAC

mRNA sequence

GGAGCTTATTCATCAAAAGCACTAAAATACACATATTATTAATATATGCATTTTTTCTATTTACAAAATAAAATGTGGGAGTGTTGAGTGTGAGAGTGGATAAAACGAAGCAGATAGAGAGAATATGGCACTGGCAATGGCCATGGCGCTACGTAGGCTTTCTTCTTCCATTCACAGCCCCCTTCGTCACCTCCATGGAGCCTCTTTCCGTTATCTGTCTTCTTTGCCCAACGAAGCTGTCTATGATAAGGAGAGACCGCGCGTTCCGTGGCCGAAACAACTGAACGATCCGCTCGAGGTAATTGATCCAGAAATTGCGGACATAATCGAACTCGAGAAGGCCAGGCAATGGAAGGGCCTGGAACTCATACCATCTGAGAACTTCACTTCCGTCTCTGTGATGCAAGCAGTTGGATCTGTTATGACTAACAAATATAGTGAAGGTTATCCTGGGGCTAGATACTATGGAGGAAACGAGTACATCGACATGGCCGAATCCTTGTGTCAGAAACGTGCTCTGGAAGCATTTCGATTGGATCCAGAAAAATGGGGAGTGAATGTTCAATCTTTATCTGGCTCTCCATCCAATTTTCAAGTTTATACTGCATTGCTAAAACCCCACGAAAGAATTATGGCGCTTGATCTTCCTCATGGTGGACATCTCTCTCATGGTTACCAGACTGATACCAAAAAGATCTCTGCAGTATCTATATTTTTTGAGACAATGCCATACAGATTGGATGAGAGCACGGGCTATATTGACTATGATCAGTTGGAGAGAAGTGCTACTCTTTTCCGGCCTAAGTTAATTGTTGCTGGTGCCAGTGCCTATGCACGCTTATATGATTATGCACGTATACGCAAGGTCTGTGATAAACAAAAAGCTATAATGTTGGCAGATATGGCACATATCAGTGGATTGGTTGCAGCTGATGTTATCCCTTCACCATTTGAATATGCTGATATTGTAACCACCACGACTCACAAGTCACTTCGTGGCCCACGTGGAGCCATGATATTTTTTAGGAAAGGAGTGAAAGAGATAAATAAACAAGGGCGGGAGGTGTTATATGACTACGAGGACAAAATCAATCAGGCTGTCTTTCCTGGTCTACAAGGTGGTCCGCACAACCACACAATTACTGGTTTAGCAGTTGCACTGAAACAGGCAACAACTCCAGAATACAAAGCCTATCAAGAGCAAGTTCTTAGGAATTGCTCAAATTTTGCACAGTCTCTCGCTGAGAAAGGCTATGAACTTGTATCTGGTGGAACTGATAATCATCTGGTTCTAGTAAATTTGAAGAATAAGGGTATTGATGGTTCAAGAGTTGAAAAGGTGCTGGAATCAGTTCATATCGCTGCCAACAAAAACACTGTTCCTGGAGATGTCTCAGCTATGGTTCCCGGTGGTATCAGGATGGGAACTCCCGCTCTTACCTCCAGAGGATTTGTTGAGGAAGACTTCGCTAAGGTAGCAGAGTTCTTTGATGCTGCTGTGAACATAGCCGTAAAGATCAAAGCAGAAACCAAAGGAACAAAATTGAAGGACTTTCTTACCACAATGGAATCAACTCCTTACTTCCAGTCCGAGATCAAAAATCTAAAACAGGATGTTGAGGAATATGCAAAGAAATTTCCTACTATTGGTTTTGAAAAGGAAACAATGAAATACAAGAGCTGAGAGAAAAAGGCCTGCCTGGGATGAAGCAGAAAAGAAATGGTGACGAAGAAACTAAAGGGTCATGTGTCAAGTTTGTAAGTTTTAATTATTTGAAATGCACAAATTCAGCAGATCATTTTGATTGGGTCTGAGATGTATATATTGCATTTTGAAGTTATCTACGAAAATAAACTGGTTTGATTCCAGTTATTTCTTTCCCACTAGTTTGTCATCATTTGATCTATCTTTTTTTCTTTTATTAATCCTATTAACTTTGTTATTATGACCTTAC

Coding sequence (CDS)

ATGGCACTGGCAATGGCCATGGCGCTACGTAGGCTTTCTTCTTCCATTCACAGCCCCCTTCGTCACCTCCATGGAGCCTCTTTCCGTTATCTGTCTTCTTTGCCCAACGAAGCTGTCTATGATAAGGAGAGACCGCGCGTTCCGTGGCCGAAACAACTGAACGATCCGCTCGAGGTAATTGATCCAGAAATTGCGGACATAATCGAACTCGAGAAGGCCAGGCAATGGAAGGGCCTGGAACTCATACCATCTGAGAACTTCACTTCCGTCTCTGTGATGCAAGCAGTTGGATCTGTTATGACTAACAAATATAGTGAAGGTTATCCTGGGGCTAGATACTATGGAGGAAACGAGTACATCGACATGGCCGAATCCTTGTGTCAGAAACGTGCTCTGGAAGCATTTCGATTGGATCCAGAAAAATGGGGAGTGAATGTTCAATCTTTATCTGGCTCTCCATCCAATTTTCAAGTTTATACTGCATTGCTAAAACCCCACGAAAGAATTATGGCGCTTGATCTTCCTCATGGTGGACATCTCTCTCATGGTTACCAGACTGATACCAAAAAGATCTCTGCAGTATCTATATTTTTTGAGACAATGCCATACAGATTGGATGAGAGCACGGGCTATATTGACTATGATCAGTTGGAGAGAAGTGCTACTCTTTTCCGGCCTAAGTTAATTGTTGCTGGTGCCAGTGCCTATGCACGCTTATATGATTATGCACGTATACGCAAGGTCTGTGATAAACAAAAAGCTATAATGTTGGCAGATATGGCACATATCAGTGGATTGGTTGCAGCTGATGTTATCCCTTCACCATTTGAATATGCTGATATTGTAACCACCACGACTCACAAGTCACTTCGTGGCCCACGTGGAGCCATGATATTTTTTAGGAAAGGAGTGAAAGAGATAAATAAACAAGGGCGGGAGGTGTTATATGACTACGAGGACAAAATCAATCAGGCTGTCTTTCCTGGTCTACAAGGTGGTCCGCACAACCACACAATTACTGGTTTAGCAGTTGCACTGAAACAGGCAACAACTCCAGAATACAAAGCCTATCAAGAGCAAGTTCTTAGGAATTGCTCAAATTTTGCACAGTCTCTCGCTGAGAAAGGCTATGAACTTGTATCTGGTGGAACTGATAATCATCTGGTTCTAGTAAATTTGAAGAATAAGGGTATTGATGGTTCAAGAGTTGAAAAGGTGCTGGAATCAGTTCATATCGCTGCCAACAAAAACACTGTTCCTGGAGATGTCTCAGCTATGGTTCCCGGTGGTATCAGGATGGGAACTCCCGCTCTTACCTCCAGAGGATTTGTTGAGGAAGACTTCGCTAAGGTAGCAGAGTTCTTTGATGCTGCTGTGAACATAGCCGTAAAGATCAAAGCAGAAACCAAAGGAACAAAATTGAAGGACTTTCTTACCACAATGGAATCAACTCCTTACTTCCAGTCCGAGATCAAAAATCTAAAACAGGATGTTGAGGAATATGCAAAGAAATTTCCTACTATTGGTTTTGAAAAGGAAACAATGAAATACAAGAGCTGA

Protein sequence

MALAMAMALRRLSSSIHSPLRHLHGASFRYLSSLPNEAVYDKERPRVPWPKQLNDPLEVIDPEIADIIELEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHERIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSATLFRPKLIVAGASAYARLYDYARIRKVCDKQKAIMLADMAHISGLVAADVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKGVKEINKQGREVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQVLRNCSNFAQSLAEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEFFDAAVNIAVKIKAETKGTKLKDFLTTMESTPYFQSEIKNLKQDVEEYAKKFPTIGFEKETMKYKS*
Homology
BLAST of CsGy6G029090 vs. ExPASy Swiss-Prot
Match: P50433 (Serine hydroxymethyltransferase, mitochondrial OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 915.2 bits (2364), Expect = 3.3e-265
Identity = 452/518 (87.26%), Postives = 488/518 (94.21%), Query Frame = 0

Query: 3   LAMAMALRRLSSSIHSPLRHLH-GASFRYLSSLPNEAVYDKERPRVPWPKQLNDPLEVID 62
           +AMA+ALRRLS+++  P++ L+ G S  Y+SSLPNEAVYDKE+  V WPKQLN PLEV+D
Sbjct: 1   MAMAIALRRLSATVDKPVKSLYNGGSLYYMSSLPNEAVYDKEKSGVAWPKQLNAPLEVVD 60

Query: 63  PEIADIIELEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYID 122
           PEIADIIE EKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYID
Sbjct: 61  PEIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYID 120

Query: 123 MAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHERIMALDLPHGGHLS 182
           MAE+LCQKRALEAFRLDP KWGVNVQ LSGSP+NFQVYTALLKPHERIMALDLPHGGHLS
Sbjct: 121 MAETLCQKRALEAFRLDPAKWGVNVQPLSGSPANFQVYTALLKPHERIMALDLPHGGHLS 180

Query: 183 HGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSATLFRPKLIVAGASAYARLYD 242
           HGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLE+SATLFRPKLIVAGASAYARLYD
Sbjct: 181 HGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLEKSATLFRPKLIVAGASAYARLYD 240

Query: 243 YARIRKVCDKQKAIMLADMAHISGLVAADVIPSPFEYADIVTTTTHKSLRGPRGAMIFFR 302
           Y RIRKVC+KQKAI+LADMAHISGLVAA VIPSPF+YAD+VTTTTHKSLRGPRGAMIF+R
Sbjct: 241 YDRIRKVCNKQKAILLADMAHISGLVAAGVIPSPFDYADVVTTTTHKSLRGPRGAMIFYR 300

Query: 303 KGVKEINKQGREVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQV 362
           KGVKE+NKQG+EV YDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEY+AYQEQV
Sbjct: 301 KGVKEVNKQGKEVFYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYRAYQEQV 360

Query: 363 LRNCSNFAQSLAEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPG 422
           L N S FAQ+L EKGYELVSGGTDNHLVLVN+KNKGIDGSRVEKVLE+VHIAANKNTVPG
Sbjct: 361 LSNSSKFAQALGEKGYELVSGGTDNHLVLVNMKNKGIDGSRVEKVLEAVHIAANKNTVPG 420

Query: 423 DVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEFFDAAVNIAVKIKAETKGTKLKDFLTTM 482
           DVSAMVPGGIRMGTPALTSRGF+EEDF KVA+FFDAAV IAVK+KAET+GTKLKDF+ T+
Sbjct: 421 DVSAMVPGGIRMGTPALTSRGFLEEDFVKVADFFDAAVKIAVKVKAETQGTKLKDFVATL 480

Query: 483 ESTPYFQSEIKNLKQDVEEYAKKFPTIGFEKETMKYKS 520
           ES+   +SEI  L+ DVEEYAK+FPTIGFEKETMKYK+
Sbjct: 481 ESSAPIKSEIAKLRHDVEEYAKQFPTIGFEKETMKYKN 518

BLAST of CsGy6G029090 vs. ExPASy Swiss-Prot
Match: P34899 (Serine hydroxymethyltransferase, mitochondrial OS=Pisum sativum OX=3888 PE=1 SV=1)

HSP 1 Score: 912.5 bits (2357), Expect = 2.1e-264
Identity = 451/516 (87.40%), Postives = 486/516 (94.19%), Query Frame = 0

Query: 3   LAMAMALRRLSSSIHSPLRHLHGA-SFRYLSSLPNEAVYDKERPRVPWPKQLNDPLEVID 62
           +AMAMALR+LSSS++   R L  A S  Y SSLP+EAVYDKE PRV WPKQLN PLEVID
Sbjct: 1   MAMAMALRKLSSSVNKSSRPLFSASSLYYKSSLPDEAVYDKENPRVTWPKQLNSPLEVID 60

Query: 63  PEIADIIELEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYID 122
           PEIADIIELEKARQWKGLELIPSENFTS+SVMQAVGSVMTNKYSEGYPGARYYGGNEYID
Sbjct: 61  PEIADIIELEKARQWKGLELIPSENFTSLSVMQAVGSVMTNKYSEGYPGARYYGGNEYID 120

Query: 123 MAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHERIMALDLPHGGHLS 182
           MAE+LCQKRALEAFRLDP KWGVNVQ LSGSPSNFQVYTALLKPH+RIMALDLPHGGHLS
Sbjct: 121 MAETLCQKRALEAFRLDPAKWGVNVQPLSGSPSNFQVYTALLKPHDRIMALDLPHGGHLS 180

Query: 183 HGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSATLFRPKLIVAGASAYARLYD 242
           HGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLE+SATLFRPKLIVAGASAYARLYD
Sbjct: 181 HGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLEKSATLFRPKLIVAGASAYARLYD 240

Query: 243 YARIRKVCDKQKAIMLADMAHISGLVAADVIPSPFEYADIVTTTTHKSLRGPRGAMIFFR 302
           YARIRKVCDKQKA++LADMAHISGLVAA VIPSPF+YAD+VTTTTHKSLRGPRGAMIFFR
Sbjct: 241 YARIRKVCDKQKAVLLADMAHISGLVAAGVIPSPFDYADVVTTTTHKSLRGPRGAMIFFR 300

Query: 303 KGVKEINKQGREVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQV 362
           KG+KE+NKQG+EV YDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEY+AYQEQV
Sbjct: 301 KGLKEVNKQGKEVFYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYRAYQEQV 360

Query: 363 LRNCSNFAQSLAEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPG 422
           L N S FA++L+EKGY+LVSGGT+NHLVLVNLKNKGIDGSRVEKVLE VHIAANKNTVPG
Sbjct: 361 LSNSSKFAKALSEKGYDLVSGGTENHLVLVNLKNKGIDGSRVEKVLELVHIAANKNTVPG 420

Query: 423 DVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEFFDAAVNIAVKIKAETKGTKLKDFLTTM 482
           DVSAMVPGGIRMGTPALTSRGFVEEDF KVAE+FDAAV++A+K+KAE+KGTKLKDF+  +
Sbjct: 421 DVSAMVPGGIRMGTPALTSRGFVEEDFVKVAEYFDAAVSLALKVKAESKGTKLKDFVEAL 480

Query: 483 ESTPYFQSEIKNLKQDVEEYAKKFPTIGFEKETMKY 518
           +++ Y QSEI  LK DVEE+AK+FPTIGFEK TMKY
Sbjct: 481 QTSSYVQSEISKLKHDVEEFAKQFPTIGFEKATMKY 516

BLAST of CsGy6G029090 vs. ExPASy Swiss-Prot
Match: Q9SZJ5 (Serine hydroxymethyltransferase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SHM1 PE=1 SV=1)

HSP 1 Score: 904.4 bits (2336), Expect = 5.8e-262
Identity = 449/517 (86.85%), Postives = 486/517 (94.00%), Query Frame = 0

Query: 3   LAMAMALRRLSSSIHSPLRHLHGASFRYLSSLPNEAVYDKERPRVPWPKQLNDPLEVIDP 62
           +AMAMALRRLSSSI  P+R L  ++  Y+SSLP+EAV +KER RV WPKQLN PLE +DP
Sbjct: 1   MAMAMALRRLSSSIDKPIRPLIRSTSCYMSSLPSEAVDEKERSRVTWPKQLNAPLEEVDP 60

Query: 63  EIADIIELEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDM 122
           EIADIIE EKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDM
Sbjct: 61  EIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDM 120

Query: 123 AESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHERIMALDLPHGGHLSH 182
           AE+LCQKRALEAFRLDPEKWGVNVQ LSGSP+NF VYTALLKPHERIMALDLPHGGHLSH
Sbjct: 121 AETLCQKRALEAFRLDPEKWGVNVQPLSGSPANFHVYTALLKPHERIMALDLPHGGHLSH 180

Query: 183 GYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSATLFRPKLIVAGASAYARLYDY 242
           GYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQ+E+SATLFRPKLIVAGASAYARLYDY
Sbjct: 181 GYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQMEKSATLFRPKLIVAGASAYARLYDY 240

Query: 243 ARIRKVCDKQKAIMLADMAHISGLVAADVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRK 302
           ARIRKVC+KQKA+MLADMAHISGLVAA+VIPSPF+YAD+VTTTTHKSLRGPRGAMIFFRK
Sbjct: 241 ARIRKVCNKQKAVMLADMAHISGLVAANVIPSPFDYADVVTTTTHKSLRGPRGAMIFFRK 300

Query: 303 GVKEINKQGREVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQVL 362
           GVKEINKQG+EVLYD+EDKINQAVFPGLQGGPHNHTITGLAVALKQATT EYKAYQEQVL
Sbjct: 301 GVKEINKQGKEVLYDFEDKINQAVFPGLQGGPHNHTITGLAVALKQATTSEYKAYQEQVL 360

Query: 363 RNCSNFAQSLAEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGD 422
            N + FAQ+L E+GYELVSGGTDNHLVLVNLK KGIDGSRVEKVLE+VHIA+NKNTVPGD
Sbjct: 361 SNSAKFAQTLMERGYELVSGGTDNHLVLVNLKPKGIDGSRVEKVLEAVHIASNKNTVPGD 420

Query: 423 VSAMVPGGIRMGTPALTSRGFVEEDFAKVAEFFDAAVNIAVKIKAETKGTKLKDFLTTME 482
           VSAMVPGGIRMGTPALTSRGFVEEDFAKVAE+FD AV IA+K+K+E +GTKLKDF++ ME
Sbjct: 421 VSAMVPGGIRMGTPALTSRGFVEEDFAKVAEYFDKAVTIALKVKSEAQGTKLKDFVSAME 480

Query: 483 STPYFQSEIKNLKQDVEEYAKKFPTIGFEKETMKYKS 520
           S+   QSEI  L+ +VEE+AK+FPTIGFEKETMKYK+
Sbjct: 481 SSSTIQSEIAKLRHEVEEFAKQFPTIGFEKETMKYKN 517

BLAST of CsGy6G029090 vs. ExPASy Swiss-Prot
Match: P49357 (Serine hydroxymethyltransferase 1, mitochondrial OS=Flaveria pringlei OX=4226 PE=2 SV=1)

HSP 1 Score: 897.9 bits (2319), Expect = 5.4e-260
Identity = 448/518 (86.49%), Postives = 480/518 (92.66%), Query Frame = 0

Query: 3   LAMAMALRRLSSSIHSPLRHLHGASFRY-LSSLPNEAVYDKERPRVPWPKQLNDPLEVID 62
           +AMA+ALRRLSSS   PL+ L      Y +SSLP+EAVY+KERP V WPKQLN PLEV+D
Sbjct: 1   MAMALALRRLSSSADKPLQRLFNGGHLYSMSSLPSEAVYEKERPGVTWPKQLNAPLEVVD 60

Query: 63  PEIADIIELEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYID 122
           PEIADIIELEKARQWKGLELIPSENFTS+SVMQAVGSVMTNKYSEGYPGARYYGGNEYID
Sbjct: 61  PEIADIIELEKARQWKGLELIPSENFTSLSVMQAVGSVMTNKYSEGYPGARYYGGNEYID 120

Query: 123 MAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHERIMALDLPHGGHLS 182
           MAE+LCQKRALEAFRLDP KWGVNVQ LSGSP+NF VYTALLK H+RIMALDLPHGGHLS
Sbjct: 121 MAETLCQKRALEAFRLDPAKWGVNVQPLSGSPANFHVYTALLKAHDRIMALDLPHGGHLS 180

Query: 183 HGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSATLFRPKLIVAGASAYARLYD 242
           HGYQTDTKKISAVSIFFETMPYRL+ESTGYIDYDQLE+SATLFRPKLIVAGASAYARLYD
Sbjct: 181 HGYQTDTKKISAVSIFFETMPYRLNESTGYIDYDQLEKSATLFRPKLIVAGASAYARLYD 240

Query: 243 YARIRKVCDKQKAIMLADMAHISGLVAADVIPSPFEYADIVTTTTHKSLRGPRGAMIFFR 302
           YARIRKVCDKQKAIMLADMAHISGLVAA VIPSPF+YAD+VTTTTHKSLRGPRGAMIFFR
Sbjct: 241 YARIRKVCDKQKAIMLADMAHISGLVAAGVIPSPFDYADVVTTTTHKSLRGPRGAMIFFR 300

Query: 303 KGVKEINKQGREVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQV 362
           KG+KE+NKQG+EV YDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATT EYKAYQEQV
Sbjct: 301 KGLKEVNKQGKEVFYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTAEYKAYQEQV 360

Query: 363 LRNCSNFAQSLAEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPG 422
           + N + FA++L + GYELVSGGT+NHLVLVNLKNKGIDGS+VEKVLE+VHIAANKNTVPG
Sbjct: 361 MSNSAKFAETLVKSGYELVSGGTENHLVLVNLKNKGIDGSKVEKVLEAVHIAANKNTVPG 420

Query: 423 DVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEFFDAAVNIAVKIKAETKGTKLKDFLTTM 482
           DVSAMVPGGIRMGTPALTSRGFVEEDFAKVA FFD AV +AVKIK E KGTKLKDF+T M
Sbjct: 421 DVSAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDLAVKLAVKIKGEAKGTKLKDFVTAM 480

Query: 483 ESTPYFQSEIKNLKQDVEEYAKKFPTIGFEKETMKYKS 520
           ES+   QSEI  L+ DVEEYAK+FPTIGFEKETMKYK+
Sbjct: 481 ESSA-IQSEISKLRHDVEEYAKQFPTIGFEKETMKYKN 517

BLAST of CsGy6G029090 vs. ExPASy Swiss-Prot
Match: P49358 (Serine hydroxymethyltransferase 2, mitochondrial OS=Flaveria pringlei OX=4226 PE=2 SV=1)

HSP 1 Score: 889.4 bits (2297), Expect = 1.9e-257
Identity = 446/518 (86.10%), Postives = 478/518 (92.28%), Query Frame = 0

Query: 3   LAMAMALRRLSSSIHSPLRHLHGASFRY-LSSLPNEAVYDKERPRVPWPKQLNDPLEVID 62
           +AMA ALRRLSSS + PL+ L      Y +SSLP+EAVY+KERP V WPKQLN PLEV D
Sbjct: 1   MAMASALRRLSSSSNKPLQRLFNGGHLYSMSSLPSEAVYEKERPGVTWPKQLNAPLEVGD 60

Query: 63  PEIADIIELEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYID 122
           PEIADIIELEKARQWKGLELI SENFTS+SVMQAVGSVMTNKYSEGYPGARYYGGNEYID
Sbjct: 61  PEIADIIELEKARQWKGLELILSENFTSLSVMQAVGSVMTNKYSEGYPGARYYGGNEYID 120

Query: 123 MAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHERIMALDLPHGGHLS 182
           MAE+LCQKRALEAFRLD  KWGVNVQ LSGSP+NF VYTALLK H+RIMALDLPHGGHLS
Sbjct: 121 MAETLCQKRALEAFRLDAAKWGVNVQPLSGSPANFHVYTALLKAHDRIMALDLPHGGHLS 180

Query: 183 HGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSATLFRPKLIVAGASAYARLYD 242
           HGYQTDTKKISAVSIFFETMPYRL+ESTGYIDYDQLE+SATLFRPKLIVAGASAYARLYD
Sbjct: 181 HGYQTDTKKISAVSIFFETMPYRLNESTGYIDYDQLEKSATLFRPKLIVAGASAYARLYD 240

Query: 243 YARIRKVCDKQKAIMLADMAHISGLVAADVIPSPFEYADIVTTTTHKSLRGPRGAMIFFR 302
           YARIRKVCDKQKAI+LADMAHISGLVAA VIPSPF+YAD+VTTTTHKSLRGPRGAMIFFR
Sbjct: 241 YARIRKVCDKQKAILLADMAHISGLVAAGVIPSPFDYADVVTTTTHKSLRGPRGAMIFFR 300

Query: 303 KGVKEINKQGREVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQV 362
           KGVKE+NKQG+EVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATT EYKAYQEQV
Sbjct: 301 KGVKEVNKQGKEVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTAEYKAYQEQV 360

Query: 363 LRNCSNFAQSLAEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPG 422
           + NC+ FA++L + GYELVSGGT+NHLVLVNLKNKGIDGSRVEKVLE+VHIAANKNTVPG
Sbjct: 361 MSNCAKFAETLVKSGYELVSGGTENHLVLVNLKNKGIDGSRVEKVLEAVHIAANKNTVPG 420

Query: 423 DVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEFFDAAVNIAVKIKAETKGTKLKDFLTTM 482
           DVSAMVPGGIRMGTPALTSRGFVEEDFAKVA  FD AV +AVKIK E +GTKLKDF+  M
Sbjct: 421 DVSAMVPGGIRMGTPALTSRGFVEEDFAKVAYLFDLAVKLAVKIKGEAQGTKLKDFVAAM 480

Query: 483 ESTPYFQSEIKNLKQDVEEYAKKFPTIGFEKETMKYKS 520
           +S+  FQSEI  L+ DVEEYAK+FPTIGFEKETMKYK+
Sbjct: 481 QSSA-FQSEISKLRHDVEEYAKQFPTIGFEKETMKYKN 517

BLAST of CsGy6G029090 vs. NCBI nr
Match: XP_004143436.1 (serine hydroxymethyltransferase, mitochondrial [Cucumis sativus] >KGN48681.1 hypothetical protein Csa_004067 [Cucumis sativus])

HSP 1 Score: 1032 bits (2668), Expect = 0.0
Identity = 519/519 (100.00%), Postives = 519/519 (100.00%), Query Frame = 0

Query: 1   MALAMAMALRRLSSSIHSPLRHLHGASFRYLSSLPNEAVYDKERPRVPWPKQLNDPLEVI 60
           MALAMAMALRRLSSSIHSPLRHLHGASFRYLSSLPNEAVYDKERPRVPWPKQLNDPLEVI
Sbjct: 1   MALAMAMALRRLSSSIHSPLRHLHGASFRYLSSLPNEAVYDKERPRVPWPKQLNDPLEVI 60

Query: 61  DPEIADIIELEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYI 120
           DPEIADIIELEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYI
Sbjct: 61  DPEIADIIELEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYI 120

Query: 121 DMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHERIMALDLPHGGHL 180
           DMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHERIMALDLPHGGHL
Sbjct: 121 DMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHERIMALDLPHGGHL 180

Query: 181 SHGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSATLFRPKLIVAGASAYARLY 240
           SHGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSATLFRPKLIVAGASAYARLY
Sbjct: 181 SHGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSATLFRPKLIVAGASAYARLY 240

Query: 241 DYARIRKVCDKQKAIMLADMAHISGLVAADVIPSPFEYADIVTTTTHKSLRGPRGAMIFF 300
           DYARIRKVCDKQKAIMLADMAHISGLVAADVIPSPFEYADIVTTTTHKSLRGPRGAMIFF
Sbjct: 241 DYARIRKVCDKQKAIMLADMAHISGLVAADVIPSPFEYADIVTTTTHKSLRGPRGAMIFF 300

Query: 301 RKGVKEINKQGREVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQ 360
           RKGVKEINKQGREVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQ
Sbjct: 301 RKGVKEINKQGREVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQ 360

Query: 361 VLRNCSNFAQSLAEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVP 420
           VLRNCSNFAQSLAEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVP
Sbjct: 361 VLRNCSNFAQSLAEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVP 420

Query: 421 GDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEFFDAAVNIAVKIKAETKGTKLKDFLTT 480
           GDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEFFDAAVNIAVKIKAETKGTKLKDFLTT
Sbjct: 421 GDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEFFDAAVNIAVKIKAETKGTKLKDFLTT 480

Query: 481 MESTPYFQSEIKNLKQDVEEYAKKFPTIGFEKETMKYKS 519
           MESTPYFQSEIKNLKQDVEEYAKKFPTIGFEKETMKYKS
Sbjct: 481 MESTPYFQSEIKNLKQDVEEYAKKFPTIGFEKETMKYKS 519

BLAST of CsGy6G029090 vs. NCBI nr
Match: XP_008440509.1 (PREDICTED: serine hydroxymethyltransferase, mitochondrial [Cucumis melo] >KAA0036360.1 serine hydroxymethyltransferase [Cucumis melo var. makuwa] >TYK12755.1 serine hydroxymethyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 1020 bits (2638), Expect = 0.0
Identity = 510/519 (98.27%), Postives = 515/519 (99.23%), Query Frame = 0

Query: 1   MALAMAMALRRLSSSIHSPLRHLHGASFRYLSSLPNEAVYDKERPRVPWPKQLNDPLEVI 60
           MALAMAMALRRLSSS+HSPLRHLHG SFRYLSSLPNEAVYDKERPRVPWPKQLNDPLEV+
Sbjct: 1   MALAMAMALRRLSSSVHSPLRHLHGGSFRYLSSLPNEAVYDKERPRVPWPKQLNDPLEVV 60

Query: 61  DPEIADIIELEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYI 120
           DPEIADIIELEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYI
Sbjct: 61  DPEIADIIELEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYI 120

Query: 121 DMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHERIMALDLPHGGHL 180
           DMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPH+RIMALDLPHGGHL
Sbjct: 121 DMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHDRIMALDLPHGGHL 180

Query: 181 SHGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSATLFRPKLIVAGASAYARLY 240
           SHGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSATLFRPKLIVAGASAYARLY
Sbjct: 181 SHGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSATLFRPKLIVAGASAYARLY 240

Query: 241 DYARIRKVCDKQKAIMLADMAHISGLVAADVIPSPFEYADIVTTTTHKSLRGPRGAMIFF 300
           DYARIRKVCDKQKAIMLADMAHISGLVAA VIPSPFEYAD+VTTTTHKSLRGPRGAMIFF
Sbjct: 241 DYARIRKVCDKQKAIMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFF 300

Query: 301 RKGVKEINKQGREVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQ 360
           RKGVKEINKQGREVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQ
Sbjct: 301 RKGVKEINKQGREVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQ 360

Query: 361 VLRNCSNFAQSLAEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVP 420
           VLRNCSNFAQSL EKGYELVSGGT+NHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVP
Sbjct: 361 VLRNCSNFAQSLVEKGYELVSGGTENHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVP 420

Query: 421 GDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEFFDAAVNIAVKIKAETKGTKLKDFLTT 480
           GDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEFFDAAVNIAVKIKAETKGTKLKDFLTT
Sbjct: 421 GDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEFFDAAVNIAVKIKAETKGTKLKDFLTT 480

Query: 481 MESTPYFQSEIKNLKQDVEEYAKKFPTIGFEKETMKYKS 519
           MESTPYFQSEIKNLK DVEEYAKKFPTIGFEKETMKYKS
Sbjct: 481 MESTPYFQSEIKNLKHDVEEYAKKFPTIGFEKETMKYKS 519

BLAST of CsGy6G029090 vs. NCBI nr
Match: XP_038882636.1 (serine hydroxymethyltransferase, mitochondrial [Benincasa hispida])

HSP 1 Score: 995 bits (2572), Expect = 0.0
Identity = 498/518 (96.14%), Postives = 509/518 (98.26%), Query Frame = 0

Query: 3   LAMAMALRRLSSSIHSPLRHL-HGASFRYLSSLPNEAVYDKERPRVPWPKQLNDPLEVID 62
           +A+AMALRRLSSSIHSP+RHL HG SF YLSSLPNEAVYDKERPRVPWPKQLNDPLEVID
Sbjct: 1   MALAMALRRLSSSIHSPIRHLLHGGSFCYLSSLPNEAVYDKERPRVPWPKQLNDPLEVID 60

Query: 63  PEIADIIELEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYID 122
           PEIADIIELEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYID
Sbjct: 61  PEIADIIELEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYID 120

Query: 123 MAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHERIMALDLPHGGHLS 182
           MAESLCQKRALEAF LDPEKWGVNVQSLSGSPSNFQVYTALLKPH+RIMALDLPHGGHLS
Sbjct: 121 MAESLCQKRALEAFHLDPEKWGVNVQSLSGSPSNFQVYTALLKPHDRIMALDLPHGGHLS 180

Query: 183 HGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSATLFRPKLIVAGASAYARLYD 242
           HGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSATLFRPKLIVAGASAYARLYD
Sbjct: 181 HGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSATLFRPKLIVAGASAYARLYD 240

Query: 243 YARIRKVCDKQKAIMLADMAHISGLVAADVIPSPFEYADIVTTTTHKSLRGPRGAMIFFR 302
           YARIRKVCDKQKAIMLADMAHISGLVAA VIPSPFEYAD+VTTTTHKSLRGPRGAMIFFR
Sbjct: 241 YARIRKVCDKQKAIMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFR 300

Query: 303 KGVKEINKQGREVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQV 362
           KGVKEINKQGREVLYDYE+KINQAVFPGLQGGPHNHTI GLAVALKQATTPEYKAYQEQV
Sbjct: 301 KGVKEINKQGREVLYDYEEKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQV 360

Query: 363 LRNCSNFAQSLAEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPG 422
           LRNCSNFAQSL EKGYELVSGGT+NHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPG
Sbjct: 361 LRNCSNFAQSLVEKGYELVSGGTENHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPG 420

Query: 423 DVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEFFDAAVNIAVKIKAETKGTKLKDFLTTM 482
           DVSAMVPGGIRMGTPALTSRGFVEEDFAKVA+FFDAAVNIAVKIKAETKGTKLKDFLTTM
Sbjct: 421 DVSAMVPGGIRMGTPALTSRGFVEEDFAKVADFFDAAVNIAVKIKAETKGTKLKDFLTTM 480

Query: 483 ESTPYFQSEIKNLKQDVEEYAKKFPTIGFEKETMKYKS 519
           +STPYFQSEI+ L+ DVEEYAKKFPTIGFEKETMKYKS
Sbjct: 481 QSTPYFQSEIEKLRHDVEEYAKKFPTIGFEKETMKYKS 518

BLAST of CsGy6G029090 vs. NCBI nr
Match: XP_022979027.1 (serine hydroxymethyltransferase, mitochondrial [Cucurbita maxima] >XP_022979029.1 serine hydroxymethyltransferase, mitochondrial [Cucurbita maxima])

HSP 1 Score: 980 bits (2534), Expect = 0.0
Identity = 492/518 (94.98%), Postives = 504/518 (97.30%), Query Frame = 0

Query: 3   LAMAMALRRLSSSIHSPLRHL-HGASFRYLSSLPNEAVYDKERPRVPWPKQLNDPLEVID 62
           +A+AMALRRLSSSI  P RHL HG SF YLSSLPNEAVYDKERPRVPWPKQLNDPLEVID
Sbjct: 1   MALAMALRRLSSSIDRPGRHLLHGGSFCYLSSLPNEAVYDKERPRVPWPKQLNDPLEVID 60

Query: 63  PEIADIIELEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYID 122
           PEIA+IIELEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYID
Sbjct: 61  PEIANIIELEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYID 120

Query: 123 MAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHERIMALDLPHGGHLS 182
           MAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPH+RIMALDLPHGGHLS
Sbjct: 121 MAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHDRIMALDLPHGGHLS 180

Query: 183 HGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSATLFRPKLIVAGASAYARLYD 242
           HGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQ+ERSA LFRPKLIVAGASAYARLYD
Sbjct: 181 HGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQMERSAALFRPKLIVAGASAYARLYD 240

Query: 243 YARIRKVCDKQKAIMLADMAHISGLVAADVIPSPFEYADIVTTTTHKSLRGPRGAMIFFR 302
           YARIRKVCDKQKAIMLADMAHISGLVAA VIPSPFEYAD+VTTTTHKSLRGPRGAMIFFR
Sbjct: 241 YARIRKVCDKQKAIMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFR 300

Query: 303 KGVKEINKQGREVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQV 362
           KGVKEINKQGREVLYDYEDKINQAVFPGLQGGPHNHTI GLAVALKQATTPEYKAYQEQV
Sbjct: 301 KGVKEINKQGREVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQV 360

Query: 363 LRNCSNFAQSLAEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPG 422
           LRNCS FAQSL EKGYELVSGGT+NHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPG
Sbjct: 361 LRNCSTFAQSLVEKGYELVSGGTENHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPG 420

Query: 423 DVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEFFDAAVNIAVKIKAETKGTKLKDFLTTM 482
           DVSAMVPGGIRMGTPALTSRGFVE+DFAKVAEFFDAAVN+AVKIKAETKGTKLKDFLTTM
Sbjct: 421 DVSAMVPGGIRMGTPALTSRGFVEDDFAKVAEFFDAAVNLAVKIKAETKGTKLKDFLTTM 480

Query: 483 ESTPYFQSEIKNLKQDVEEYAKKFPTIGFEKETMKYKS 519
           +STPY QSEI+ L+ DVEEYAKKFPTIGFEKETMKYKS
Sbjct: 481 QSTPYVQSEIEKLRHDVEEYAKKFPTIGFEKETMKYKS 518

BLAST of CsGy6G029090 vs. NCBI nr
Match: XP_022949662.1 (serine hydroxymethyltransferase, mitochondrial [Cucurbita moschata] >XP_022949663.1 serine hydroxymethyltransferase, mitochondrial [Cucurbita moschata])

HSP 1 Score: 980 bits (2534), Expect = 0.0
Identity = 491/518 (94.79%), Postives = 504/518 (97.30%), Query Frame = 0

Query: 3   LAMAMALRRLSSSIHSPLRHL-HGASFRYLSSLPNEAVYDKERPRVPWPKQLNDPLEVID 62
           +A+AMALRRLSSSI  P+RHL HG SF YLSSLPNEAVYDKERPRVPWPKQLNDPLEVID
Sbjct: 1   MALAMALRRLSSSIDRPVRHLLHGGSFCYLSSLPNEAVYDKERPRVPWPKQLNDPLEVID 60

Query: 63  PEIADIIELEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYID 122
           PEIA+IIELEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYID
Sbjct: 61  PEIANIIELEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYID 120

Query: 123 MAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHERIMALDLPHGGHLS 182
           MAESLCQKRALEAF LDPEKWGVNVQSLSGSPSNFQVYTALLKPH+RIMALDLPHGGHLS
Sbjct: 121 MAESLCQKRALEAFHLDPEKWGVNVQSLSGSPSNFQVYTALLKPHDRIMALDLPHGGHLS 180

Query: 183 HGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSATLFRPKLIVAGASAYARLYD 242
           HGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQ+ERSA LFRPKLIVAGASAYARLYD
Sbjct: 181 HGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQMERSAALFRPKLIVAGASAYARLYD 240

Query: 243 YARIRKVCDKQKAIMLADMAHISGLVAADVIPSPFEYADIVTTTTHKSLRGPRGAMIFFR 302
           YARIRKVCDKQKAIMLADMAHISGLVAA VIPSPFEYAD+VTTTTHKSLRGPRGAMIFFR
Sbjct: 241 YARIRKVCDKQKAIMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFR 300

Query: 303 KGVKEINKQGREVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQV 362
           KGVKEINKQGREVLYDYEDKINQAVFPGLQGGPHNHTI GLAVALKQATTPEYKAYQEQV
Sbjct: 301 KGVKEINKQGREVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQV 360

Query: 363 LRNCSNFAQSLAEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPG 422
           LRNCS FAQSL EKGYELVSGGT+NHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPG
Sbjct: 361 LRNCSTFAQSLVEKGYELVSGGTENHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPG 420

Query: 423 DVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEFFDAAVNIAVKIKAETKGTKLKDFLTTM 482
           DVSAMVPGGIRMGTPALTSRGFVE+DFAKVAEFFDAAVN+AVKIKAETKGTKLKDFLTTM
Sbjct: 421 DVSAMVPGGIRMGTPALTSRGFVEDDFAKVAEFFDAAVNLAVKIKAETKGTKLKDFLTTM 480

Query: 483 ESTPYFQSEIKNLKQDVEEYAKKFPTIGFEKETMKYKS 519
           +STPY QSEI+ L+ DVEEYAKKFPTIGFEKETMKYKS
Sbjct: 481 QSTPYVQSEIEKLRHDVEEYAKKFPTIGFEKETMKYKS 518

BLAST of CsGy6G029090 vs. ExPASy TrEMBL
Match: A0A0A0KLP6 (Serine hydroxymethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_6G497310 PE=3 SV=1)

HSP 1 Score: 1032 bits (2668), Expect = 0.0
Identity = 519/519 (100.00%), Postives = 519/519 (100.00%), Query Frame = 0

Query: 1   MALAMAMALRRLSSSIHSPLRHLHGASFRYLSSLPNEAVYDKERPRVPWPKQLNDPLEVI 60
           MALAMAMALRRLSSSIHSPLRHLHGASFRYLSSLPNEAVYDKERPRVPWPKQLNDPLEVI
Sbjct: 1   MALAMAMALRRLSSSIHSPLRHLHGASFRYLSSLPNEAVYDKERPRVPWPKQLNDPLEVI 60

Query: 61  DPEIADIIELEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYI 120
           DPEIADIIELEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYI
Sbjct: 61  DPEIADIIELEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYI 120

Query: 121 DMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHERIMALDLPHGGHL 180
           DMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHERIMALDLPHGGHL
Sbjct: 121 DMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHERIMALDLPHGGHL 180

Query: 181 SHGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSATLFRPKLIVAGASAYARLY 240
           SHGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSATLFRPKLIVAGASAYARLY
Sbjct: 181 SHGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSATLFRPKLIVAGASAYARLY 240

Query: 241 DYARIRKVCDKQKAIMLADMAHISGLVAADVIPSPFEYADIVTTTTHKSLRGPRGAMIFF 300
           DYARIRKVCDKQKAIMLADMAHISGLVAADVIPSPFEYADIVTTTTHKSLRGPRGAMIFF
Sbjct: 241 DYARIRKVCDKQKAIMLADMAHISGLVAADVIPSPFEYADIVTTTTHKSLRGPRGAMIFF 300

Query: 301 RKGVKEINKQGREVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQ 360
           RKGVKEINKQGREVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQ
Sbjct: 301 RKGVKEINKQGREVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQ 360

Query: 361 VLRNCSNFAQSLAEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVP 420
           VLRNCSNFAQSLAEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVP
Sbjct: 361 VLRNCSNFAQSLAEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVP 420

Query: 421 GDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEFFDAAVNIAVKIKAETKGTKLKDFLTT 480
           GDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEFFDAAVNIAVKIKAETKGTKLKDFLTT
Sbjct: 421 GDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEFFDAAVNIAVKIKAETKGTKLKDFLTT 480

Query: 481 MESTPYFQSEIKNLKQDVEEYAKKFPTIGFEKETMKYKS 519
           MESTPYFQSEIKNLKQDVEEYAKKFPTIGFEKETMKYKS
Sbjct: 481 MESTPYFQSEIKNLKQDVEEYAKKFPTIGFEKETMKYKS 519

BLAST of CsGy6G029090 vs. ExPASy TrEMBL
Match: A0A5A7SZ46 (Serine hydroxymethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G003430 PE=3 SV=1)

HSP 1 Score: 1020 bits (2638), Expect = 0.0
Identity = 510/519 (98.27%), Postives = 515/519 (99.23%), Query Frame = 0

Query: 1   MALAMAMALRRLSSSIHSPLRHLHGASFRYLSSLPNEAVYDKERPRVPWPKQLNDPLEVI 60
           MALAMAMALRRLSSS+HSPLRHLHG SFRYLSSLPNEAVYDKERPRVPWPKQLNDPLEV+
Sbjct: 1   MALAMAMALRRLSSSVHSPLRHLHGGSFRYLSSLPNEAVYDKERPRVPWPKQLNDPLEVV 60

Query: 61  DPEIADIIELEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYI 120
           DPEIADIIELEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYI
Sbjct: 61  DPEIADIIELEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYI 120

Query: 121 DMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHERIMALDLPHGGHL 180
           DMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPH+RIMALDLPHGGHL
Sbjct: 121 DMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHDRIMALDLPHGGHL 180

Query: 181 SHGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSATLFRPKLIVAGASAYARLY 240
           SHGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSATLFRPKLIVAGASAYARLY
Sbjct: 181 SHGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSATLFRPKLIVAGASAYARLY 240

Query: 241 DYARIRKVCDKQKAIMLADMAHISGLVAADVIPSPFEYADIVTTTTHKSLRGPRGAMIFF 300
           DYARIRKVCDKQKAIMLADMAHISGLVAA VIPSPFEYAD+VTTTTHKSLRGPRGAMIFF
Sbjct: 241 DYARIRKVCDKQKAIMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFF 300

Query: 301 RKGVKEINKQGREVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQ 360
           RKGVKEINKQGREVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQ
Sbjct: 301 RKGVKEINKQGREVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQ 360

Query: 361 VLRNCSNFAQSLAEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVP 420
           VLRNCSNFAQSL EKGYELVSGGT+NHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVP
Sbjct: 361 VLRNCSNFAQSLVEKGYELVSGGTENHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVP 420

Query: 421 GDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEFFDAAVNIAVKIKAETKGTKLKDFLTT 480
           GDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEFFDAAVNIAVKIKAETKGTKLKDFLTT
Sbjct: 421 GDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEFFDAAVNIAVKIKAETKGTKLKDFLTT 480

Query: 481 MESTPYFQSEIKNLKQDVEEYAKKFPTIGFEKETMKYKS 519
           MESTPYFQSEIKNLK DVEEYAKKFPTIGFEKETMKYKS
Sbjct: 481 MESTPYFQSEIKNLKHDVEEYAKKFPTIGFEKETMKYKS 519

BLAST of CsGy6G029090 vs. ExPASy TrEMBL
Match: A0A1S3B1B2 (Serine hydroxymethyltransferase OS=Cucumis melo OX=3656 GN=LOC103484914 PE=3 SV=1)

HSP 1 Score: 1020 bits (2638), Expect = 0.0
Identity = 510/519 (98.27%), Postives = 515/519 (99.23%), Query Frame = 0

Query: 1   MALAMAMALRRLSSSIHSPLRHLHGASFRYLSSLPNEAVYDKERPRVPWPKQLNDPLEVI 60
           MALAMAMALRRLSSS+HSPLRHLHG SFRYLSSLPNEAVYDKERPRVPWPKQLNDPLEV+
Sbjct: 1   MALAMAMALRRLSSSVHSPLRHLHGGSFRYLSSLPNEAVYDKERPRVPWPKQLNDPLEVV 60

Query: 61  DPEIADIIELEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYI 120
           DPEIADIIELEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYI
Sbjct: 61  DPEIADIIELEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYI 120

Query: 121 DMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHERIMALDLPHGGHL 180
           DMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPH+RIMALDLPHGGHL
Sbjct: 121 DMAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHDRIMALDLPHGGHL 180

Query: 181 SHGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSATLFRPKLIVAGASAYARLY 240
           SHGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSATLFRPKLIVAGASAYARLY
Sbjct: 181 SHGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSATLFRPKLIVAGASAYARLY 240

Query: 241 DYARIRKVCDKQKAIMLADMAHISGLVAADVIPSPFEYADIVTTTTHKSLRGPRGAMIFF 300
           DYARIRKVCDKQKAIMLADMAHISGLVAA VIPSPFEYAD+VTTTTHKSLRGPRGAMIFF
Sbjct: 241 DYARIRKVCDKQKAIMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFF 300

Query: 301 RKGVKEINKQGREVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQ 360
           RKGVKEINKQGREVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQ
Sbjct: 301 RKGVKEINKQGREVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQ 360

Query: 361 VLRNCSNFAQSLAEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVP 420
           VLRNCSNFAQSL EKGYELVSGGT+NHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVP
Sbjct: 361 VLRNCSNFAQSLVEKGYELVSGGTENHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVP 420

Query: 421 GDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEFFDAAVNIAVKIKAETKGTKLKDFLTT 480
           GDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEFFDAAVNIAVKIKAETKGTKLKDFLTT
Sbjct: 421 GDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEFFDAAVNIAVKIKAETKGTKLKDFLTT 480

Query: 481 MESTPYFQSEIKNLKQDVEEYAKKFPTIGFEKETMKYKS 519
           MESTPYFQSEIKNLK DVEEYAKKFPTIGFEKETMKYKS
Sbjct: 481 MESTPYFQSEIKNLKHDVEEYAKKFPTIGFEKETMKYKS 519

BLAST of CsGy6G029090 vs. ExPASy TrEMBL
Match: A0A6J1IMP0 (Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111478793 PE=3 SV=1)

HSP 1 Score: 980 bits (2534), Expect = 0.0
Identity = 492/518 (94.98%), Postives = 504/518 (97.30%), Query Frame = 0

Query: 3   LAMAMALRRLSSSIHSPLRHL-HGASFRYLSSLPNEAVYDKERPRVPWPKQLNDPLEVID 62
           +A+AMALRRLSSSI  P RHL HG SF YLSSLPNEAVYDKERPRVPWPKQLNDPLEVID
Sbjct: 1   MALAMALRRLSSSIDRPGRHLLHGGSFCYLSSLPNEAVYDKERPRVPWPKQLNDPLEVID 60

Query: 63  PEIADIIELEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYID 122
           PEIA+IIELEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYID
Sbjct: 61  PEIANIIELEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYID 120

Query: 123 MAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHERIMALDLPHGGHLS 182
           MAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPH+RIMALDLPHGGHLS
Sbjct: 121 MAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHDRIMALDLPHGGHLS 180

Query: 183 HGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSATLFRPKLIVAGASAYARLYD 242
           HGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQ+ERSA LFRPKLIVAGASAYARLYD
Sbjct: 181 HGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQMERSAALFRPKLIVAGASAYARLYD 240

Query: 243 YARIRKVCDKQKAIMLADMAHISGLVAADVIPSPFEYADIVTTTTHKSLRGPRGAMIFFR 302
           YARIRKVCDKQKAIMLADMAHISGLVAA VIPSPFEYAD+VTTTTHKSLRGPRGAMIFFR
Sbjct: 241 YARIRKVCDKQKAIMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFR 300

Query: 303 KGVKEINKQGREVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQV 362
           KGVKEINKQGREVLYDYEDKINQAVFPGLQGGPHNHTI GLAVALKQATTPEYKAYQEQV
Sbjct: 301 KGVKEINKQGREVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQV 360

Query: 363 LRNCSNFAQSLAEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPG 422
           LRNCS FAQSL EKGYELVSGGT+NHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPG
Sbjct: 361 LRNCSTFAQSLVEKGYELVSGGTENHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPG 420

Query: 423 DVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEFFDAAVNIAVKIKAETKGTKLKDFLTTM 482
           DVSAMVPGGIRMGTPALTSRGFVE+DFAKVAEFFDAAVN+AVKIKAETKGTKLKDFLTTM
Sbjct: 421 DVSAMVPGGIRMGTPALTSRGFVEDDFAKVAEFFDAAVNLAVKIKAETKGTKLKDFLTTM 480

Query: 483 ESTPYFQSEIKNLKQDVEEYAKKFPTIGFEKETMKYKS 519
           +STPY QSEI+ L+ DVEEYAKKFPTIGFEKETMKYKS
Sbjct: 481 QSTPYVQSEIEKLRHDVEEYAKKFPTIGFEKETMKYKS 518

BLAST of CsGy6G029090 vs. ExPASy TrEMBL
Match: A0A6J1GCP5 (Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111452985 PE=3 SV=1)

HSP 1 Score: 980 bits (2534), Expect = 0.0
Identity = 491/518 (94.79%), Postives = 504/518 (97.30%), Query Frame = 0

Query: 3   LAMAMALRRLSSSIHSPLRHL-HGASFRYLSSLPNEAVYDKERPRVPWPKQLNDPLEVID 62
           +A+AMALRRLSSSI  P+RHL HG SF YLSSLPNEAVYDKERPRVPWPKQLNDPLEVID
Sbjct: 1   MALAMALRRLSSSIDRPVRHLLHGGSFCYLSSLPNEAVYDKERPRVPWPKQLNDPLEVID 60

Query: 63  PEIADIIELEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYID 122
           PEIA+IIELEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYID
Sbjct: 61  PEIANIIELEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYID 120

Query: 123 MAESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHERIMALDLPHGGHLS 182
           MAESLCQKRALEAF LDPEKWGVNVQSLSGSPSNFQVYTALLKPH+RIMALDLPHGGHLS
Sbjct: 121 MAESLCQKRALEAFHLDPEKWGVNVQSLSGSPSNFQVYTALLKPHDRIMALDLPHGGHLS 180

Query: 183 HGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSATLFRPKLIVAGASAYARLYD 242
           HGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQ+ERSA LFRPKLIVAGASAYARLYD
Sbjct: 181 HGYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQMERSAALFRPKLIVAGASAYARLYD 240

Query: 243 YARIRKVCDKQKAIMLADMAHISGLVAADVIPSPFEYADIVTTTTHKSLRGPRGAMIFFR 302
           YARIRKVCDKQKAIMLADMAHISGLVAA VIPSPFEYAD+VTTTTHKSLRGPRGAMIFFR
Sbjct: 241 YARIRKVCDKQKAIMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFR 300

Query: 303 KGVKEINKQGREVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQV 362
           KGVKEINKQGREVLYDYEDKINQAVFPGLQGGPHNHTI GLAVALKQATTPEYKAYQEQV
Sbjct: 301 KGVKEINKQGREVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQV 360

Query: 363 LRNCSNFAQSLAEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPG 422
           LRNCS FAQSL EKGYELVSGGT+NHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPG
Sbjct: 361 LRNCSTFAQSLVEKGYELVSGGTENHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPG 420

Query: 423 DVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEFFDAAVNIAVKIKAETKGTKLKDFLTTM 482
           DVSAMVPGGIRMGTPALTSRGFVE+DFAKVAEFFDAAVN+AVKIKAETKGTKLKDFLTTM
Sbjct: 421 DVSAMVPGGIRMGTPALTSRGFVEDDFAKVAEFFDAAVNLAVKIKAETKGTKLKDFLTTM 480

Query: 483 ESTPYFQSEIKNLKQDVEEYAKKFPTIGFEKETMKYKS 519
           +STPY QSEI+ L+ DVEEYAKKFPTIGFEKETMKYKS
Sbjct: 481 QSTPYVQSEIEKLRHDVEEYAKKFPTIGFEKETMKYKS 518

BLAST of CsGy6G029090 vs. TAIR 10
Match: AT4G37930.1 (serine transhydroxymethyltransferase 1 )

HSP 1 Score: 904.4 bits (2336), Expect = 4.1e-263
Identity = 449/517 (86.85%), Postives = 486/517 (94.00%), Query Frame = 0

Query: 3   LAMAMALRRLSSSIHSPLRHLHGASFRYLSSLPNEAVYDKERPRVPWPKQLNDPLEVIDP 62
           +AMAMALRRLSSSI  P+R L  ++  Y+SSLP+EAV +KER RV WPKQLN PLE +DP
Sbjct: 1   MAMAMALRRLSSSIDKPIRPLIRSTSCYMSSLPSEAVDEKERSRVTWPKQLNAPLEEVDP 60

Query: 63  EIADIIELEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDM 122
           EIADIIE EKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDM
Sbjct: 61  EIADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDM 120

Query: 123 AESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHERIMALDLPHGGHLSH 182
           AE+LCQKRALEAFRLDPEKWGVNVQ LSGSP+NF VYTALLKPHERIMALDLPHGGHLSH
Sbjct: 121 AETLCQKRALEAFRLDPEKWGVNVQPLSGSPANFHVYTALLKPHERIMALDLPHGGHLSH 180

Query: 183 GYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSATLFRPKLIVAGASAYARLYDY 242
           GYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQ+E+SATLFRPKLIVAGASAYARLYDY
Sbjct: 181 GYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQMEKSATLFRPKLIVAGASAYARLYDY 240

Query: 243 ARIRKVCDKQKAIMLADMAHISGLVAADVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRK 302
           ARIRKVC+KQKA+MLADMAHISGLVAA+VIPSPF+YAD+VTTTTHKSLRGPRGAMIFFRK
Sbjct: 241 ARIRKVCNKQKAVMLADMAHISGLVAANVIPSPFDYADVVTTTTHKSLRGPRGAMIFFRK 300

Query: 303 GVKEINKQGREVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQVL 362
           GVKEINKQG+EVLYD+EDKINQAVFPGLQGGPHNHTITGLAVALKQATT EYKAYQEQVL
Sbjct: 301 GVKEINKQGKEVLYDFEDKINQAVFPGLQGGPHNHTITGLAVALKQATTSEYKAYQEQVL 360

Query: 363 RNCSNFAQSLAEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGD 422
            N + FAQ+L E+GYELVSGGTDNHLVLVNLK KGIDGSRVEKVLE+VHIA+NKNTVPGD
Sbjct: 361 SNSAKFAQTLMERGYELVSGGTDNHLVLVNLKPKGIDGSRVEKVLEAVHIASNKNTVPGD 420

Query: 423 VSAMVPGGIRMGTPALTSRGFVEEDFAKVAEFFDAAVNIAVKIKAETKGTKLKDFLTTME 482
           VSAMVPGGIRMGTPALTSRGFVEEDFAKVAE+FD AV IA+K+K+E +GTKLKDF++ ME
Sbjct: 421 VSAMVPGGIRMGTPALTSRGFVEEDFAKVAEYFDKAVTIALKVKSEAQGTKLKDFVSAME 480

Query: 483 STPYFQSEIKNLKQDVEEYAKKFPTIGFEKETMKYKS 520
           S+   QSEI  L+ +VEE+AK+FPTIGFEKETMKYK+
Sbjct: 481 SSSTIQSEIAKLRHEVEEFAKQFPTIGFEKETMKYKN 517

BLAST of CsGy6G029090 vs. TAIR 10
Match: AT5G26780.1 (serine hydroxymethyltransferase 2 )

HSP 1 Score: 884.0 bits (2283), Expect = 5.8e-257
Identity = 437/516 (84.69%), Postives = 477/516 (92.44%), Query Frame = 0

Query: 5   MAMALRRLSSSIHSPLRHL--HGASFRYLSSLPNEAVYDKERPRVPWPKQLNDPLEVIDP 64
           MA+ALRRLSSS+  P+  L  +G S R++SSL   A+ + E+ R  W KQLN  L+ IDP
Sbjct: 1   MALALRRLSSSVKKPISLLSSNGGSLRFMSSLSTAAMAESEKSRSSWIKQLNASLDEIDP 60

Query: 65  EIADIIELEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDM 124
           E+ADIIELEKARQWKG ELIPSENFTS+SVMQAVGSVMTNKYSEGYPGARYYGGNEYIDM
Sbjct: 61  EVADIIELEKARQWKGFELIPSENFTSLSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDM 120

Query: 125 AESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHERIMALDLPHGGHLSH 184
           AE+LCQKRALEAF+LDP KWGVNVQSLSGSP+NFQVYTALLKPHERIMALDLPHGGHLSH
Sbjct: 121 AETLCQKRALEAFQLDPSKWGVNVQSLSGSPANFQVYTALLKPHERIMALDLPHGGHLSH 180

Query: 185 GYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSATLFRPKLIVAGASAYARLYDY 244
           GYQTDTKKISAVSIFFETMPYRLDE+TGYIDYDQLE+SA LFRPKLIVAGASAYARLYDY
Sbjct: 181 GYQTDTKKISAVSIFFETMPYRLDENTGYIDYDQLEKSAVLFRPKLIVAGASAYARLYDY 240

Query: 245 ARIRKVCDKQKAIMLADMAHISGLVAADVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRK 304
           ARIRKVC+KQKA+MLADMAHISGLVAA VIPSPFEYAD+VTTTTHKSLRGPRGAMIFFRK
Sbjct: 241 ARIRKVCNKQKAVMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFRK 300

Query: 305 GVKEINKQGREVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQVL 364
           G+KEINKQG+EV+YDYED+INQAVFPGLQGGPHNHTITGLAVALKQA TPEYKAYQ+QVL
Sbjct: 301 GLKEINKQGKEVMYDYEDRINQAVFPGLQGGPHNHTITGLAVALKQARTPEYKAYQDQVL 360

Query: 365 RNCSNFAQSLAEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGD 424
           RNCS FA++L  KGY+LVSGGTDNHLVLVNLKNKGIDGSRVEKVLE VHIAANKNTVPGD
Sbjct: 361 RNCSKFAETLLAKGYDLVSGGTDNHLVLVNLKNKGIDGSRVEKVLELVHIAANKNTVPGD 420

Query: 425 VSAMVPGGIRMGTPALTSRGFVEEDFAKVAEFFDAAVNIAVKIKAETKGTKLKDFLTTME 484
           VSAMVPGGIRMGTPALTSRGF+EEDFAKVAE+FD AV IA+KIKAE++GTKLKDF+ TM+
Sbjct: 421 VSAMVPGGIRMGTPALTSRGFIEEDFAKVAEYFDLAVKIALKIKAESQGTKLKDFVATMQ 480

Query: 485 STPYFQSEIKNLKQDVEEYAKKFPTIGFEKETMKYK 519
           S    QSE+  L++ VEEYAK+FPTIGFEKETM+YK
Sbjct: 481 SNEKLQSEMSKLREMVEEYAKQFPTIGFEKETMRYK 516

BLAST of CsGy6G029090 vs. TAIR 10
Match: AT5G26780.3 (serine hydroxymethyltransferase 2 )

HSP 1 Score: 874.8 bits (2259), Expect = 3.5e-254
Identity = 438/532 (82.33%), Postives = 477/532 (89.66%), Query Frame = 0

Query: 5   MAMALRRLSSSIHSPLRHL--HGASFRYLSSLPNEAVYDKERPRVPWPKQLNDPLEVIDP 64
           MA+ALRRLSSS+  P+  L  +G S R++SSL   A+ + E+ R  W KQLN  L+ IDP
Sbjct: 1   MALALRRLSSSVKKPISLLSSNGGSLRFMSSLSTAAMAESEKSRSSWIKQLNASLDEIDP 60

Query: 65  EIADIIELEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDM 124
           E+ADIIELEKARQWKG ELIPSENFTS+SVMQAVGSVMTNKYSEGYPGARYYGGNEYIDM
Sbjct: 61  EVADIIELEKARQWKGFELIPSENFTSLSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDM 120

Query: 125 AESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHERIMALDLPHGGHLSH 184
           AE+LCQKRALEAF+LDP KWGVNVQSLSGSP+NFQVYTALLKPHERIMALDLPHGGHLSH
Sbjct: 121 AETLCQKRALEAFQLDPSKWGVNVQSLSGSPANFQVYTALLKPHERIMALDLPHGGHLSH 180

Query: 185 GYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSATLFRPKLIVAGASAYARLYDY 244
           GYQTDTKKISAVSIFFETMPYRLDE+TGYIDYDQLE+SA LFRPKLIVAGASAYARLYDY
Sbjct: 181 GYQTDTKKISAVSIFFETMPYRLDENTGYIDYDQLEKSAVLFRPKLIVAGASAYARLYDY 240

Query: 245 ARIRKVCDKQKAIMLADMAHISGLVAADVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRK 304
           ARIRKVC+KQKA+MLADMAHISGLVAA VIPSPFEYAD+VTTTTHKSLRGPRGAMIFFRK
Sbjct: 241 ARIRKVCNKQKAVMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFRK 300

Query: 305 GVKEINKQGREVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQVL 364
           G+KEINKQG+EV+YDYED+INQAVFPGLQGGPHNHTITGLAVALKQA TPEYKAYQ+QVL
Sbjct: 301 GLKEINKQGKEVMYDYEDRINQAVFPGLQGGPHNHTITGLAVALKQARTPEYKAYQDQVL 360

Query: 365 RNCSNFA----------------QSLAEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKV 424
           RNCS FA                Q+L  KGY+LVSGGTDNHLVLVNLKNKGIDGSRVEKV
Sbjct: 361 RNCSKFAELDIRPTVIISYGLSMQTLLAKGYDLVSGGTDNHLVLVNLKNKGIDGSRVEKV 420

Query: 425 LESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEFFDAAVNIAVKIK 484
           LE VHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGF+EEDFAKVAE+FD AV IA+KIK
Sbjct: 421 LELVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFIEEDFAKVAEYFDLAVKIALKIK 480

Query: 485 AETKGTKLKDFLTTMESTPYFQSEIKNLKQDVEEYAKKFPTIGFEKETMKYK 519
           AE++GTKLKDF+ TM+S    QSE+  L++ VEEYAK+FPTIGFEKETM+YK
Sbjct: 481 AESQGTKLKDFVATMQSNEKLQSEMSKLREMVEEYAKQFPTIGFEKETMRYK 532

BLAST of CsGy6G029090 vs. TAIR 10
Match: AT5G26780.2 (serine hydroxymethyltransferase 2 )

HSP 1 Score: 874.8 bits (2259), Expect = 3.5e-254
Identity = 438/532 (82.33%), Postives = 477/532 (89.66%), Query Frame = 0

Query: 5   MAMALRRLSSSIHSPLRHL--HGASFRYLSSLPNEAVYDKERPRVPWPKQLNDPLEVIDP 64
           MA+ALRRLSSS+  P+  L  +G S R++SSL   A+ + E+ R  W KQLN  L+ IDP
Sbjct: 1   MALALRRLSSSVKKPISLLSSNGGSLRFMSSLSTAAMAESEKSRSSWIKQLNASLDEIDP 60

Query: 65  EIADIIELEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDM 124
           E+ADIIELEKARQWKG ELIPSENFTS+SVMQAVGSVMTNKYSEGYPGARYYGGNEYIDM
Sbjct: 61  EVADIIELEKARQWKGFELIPSENFTSLSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDM 120

Query: 125 AESLCQKRALEAFRLDPEKWGVNVQSLSGSPSNFQVYTALLKPHERIMALDLPHGGHLSH 184
           AE+LCQKRALEAF+LDP KWGVNVQSLSGSP+NFQVYTALLKPHERIMALDLPHGGHLSH
Sbjct: 121 AETLCQKRALEAFQLDPSKWGVNVQSLSGSPANFQVYTALLKPHERIMALDLPHGGHLSH 180

Query: 185 GYQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSATLFRPKLIVAGASAYARLYDY 244
           GYQTDTKKISAVSIFFETMPYRLDE+TGYIDYDQLE+SA LFRPKLIVAGASAYARLYDY
Sbjct: 181 GYQTDTKKISAVSIFFETMPYRLDENTGYIDYDQLEKSAVLFRPKLIVAGASAYARLYDY 240

Query: 245 ARIRKVCDKQKAIMLADMAHISGLVAADVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRK 304
           ARIRKVC+KQKA+MLADMAHISGLVAA VIPSPFEYAD+VTTTTHKSLRGPRGAMIFFRK
Sbjct: 241 ARIRKVCNKQKAVMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFRK 300

Query: 305 GVKEINKQGREVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQVL 364
           G+KEINKQG+EV+YDYED+INQAVFPGLQGGPHNHTITGLAVALKQA TPEYKAYQ+QVL
Sbjct: 301 GLKEINKQGKEVMYDYEDRINQAVFPGLQGGPHNHTITGLAVALKQARTPEYKAYQDQVL 360

Query: 365 RNCSNFA----------------QSLAEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKV 424
           RNCS FA                Q+L  KGY+LVSGGTDNHLVLVNLKNKGIDGSRVEKV
Sbjct: 361 RNCSKFAELDIRPTVIISYGLSMQTLLAKGYDLVSGGTDNHLVLVNLKNKGIDGSRVEKV 420

Query: 425 LESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEFFDAAVNIAVKIK 484
           LE VHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGF+EEDFAKVAE+FD AV IA+KIK
Sbjct: 421 LELVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFIEEDFAKVAEYFDLAVKIALKIK 480

Query: 485 AETKGTKLKDFLTTMESTPYFQSEIKNLKQDVEEYAKKFPTIGFEKETMKYK 519
           AE++GTKLKDF+ TM+S    QSE+  L++ VEEYAK+FPTIGFEKETM+YK
Sbjct: 481 AESQGTKLKDFVATMQSNEKLQSEMSKLREMVEEYAKQFPTIGFEKETMRYK 532

BLAST of CsGy6G029090 vs. TAIR 10
Match: AT4G32520.1 (serine hydroxymethyltransferase 3 )

HSP 1 Score: 573.9 bits (1478), Expect = 1.3e-163
Identity = 288/478 (60.25%), Postives = 353/478 (73.85%), Query Frame = 0

Query: 33  SLPNEAVYDKERPRVPWPKQLNDPLEVIDPEIADIIELEKARQWKGLELIPSENFTSVSV 92
           SLPN  +  KE P   +       L  +DPE+  II  EK RQ++ LELI SENFTS +V
Sbjct: 65  SLPNVEISSKEIPFEDY------GLGEVDPEVRTIITKEKDRQFRSLELIASENFTSRAV 124

Query: 93  MQAVGSVMTNKYSEGYPGARYYGGNEYIDMAESLCQKRALEAFRLDPEKWGVNVQSLSGS 152
           M+AVGS +TNKYSEG PG RYYGGNEYID  E+LCQ RAL AFRLD  KWGVNVQ LSGS
Sbjct: 125 MEAVGSCLTNKYSEGLPGKRYYGGNEYIDQLETLCQNRALAAFRLDSTKWGVNVQPLSGS 184

Query: 153 PSNFQVYTALLKPHERIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMPYRLDESTGYI 212
           P+NF VYTA+L PH+RIM LDLPHGGHLSHG+ T  +++S  SI+FE+MPYRLDESTG +
Sbjct: 185 PANFAVYTAILSPHDRIMGLDLPHGGHLSHGFMTAKRRVSGTSIYFESMPYRLDESTGIV 244

Query: 213 DYDQLERSATLFRPKLIVAGASAYARLYDYARIRKVCDKQKAIMLADMAHISGLVAADVI 272
           DYD LE++ATLFRPKLI+AGASAY+R +DY R+RK+ D   A ++ DMAHISGLVAA V+
Sbjct: 245 DYDMLEKTATLFRPKLIIAGASAYSRDFDYPRMRKIADSVGAFLMMDMAHISGLVAASVV 304

Query: 273 PSPFEYADIVTTTTHKSLRGPRGAMIFFRKGVKEINKQGREVLYDYEDKINQAVFPGLQG 332
             PFEY DIVTTTTHKSLRGPRG MIFFRK    IN        D E  +N AVFPGLQG
Sbjct: 305 ADPFEYCDIVTTTTHKSLRGPRGGMIFFRK--DPING------VDLESAVNNAVFPGLQG 364

Query: 333 GPHNHTITGLAVALKQATTPEYKAYQEQVLRNCSNFAQSLAEKGYELVSGGTDNHLVLVN 392
           GPHNHTI GLAV LK A +PE+KAYQ++V+ NC   A  L E G++LVSGG+DNHLVLV+
Sbjct: 365 GPHNHTIGGLAVCLKHAQSPEFKAYQKRVVSNCRALANRLVELGFKLVSGGSDNHLVLVD 424

Query: 393 LKNKGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVA 452
           L+  G+DG+RVEK+L+   I  NKN+VPGD SA+VPGGIR+G+PA+T+RG  E+DF  VA
Sbjct: 425 LRPMGMDGARVEKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGLSEKDFVVVA 484

Query: 453 EFFDAAVNIAVKIKAETKGTKLKDFLTTMESTPY-FQSEIKNLKQDVEEYAKKFPTIG 510
           +F    V I ++ K    G+KL+DF   + S  +  +  +K+LK+ VE +  +FP  G
Sbjct: 485 DFIKEGVEITMEAKKAAPGSKLQDFNKFVTSPEFPLKERVKSLKERVETFTSRFPIPG 528

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P504333.3e-26587.26Serine hydroxymethyltransferase, mitochondrial OS=Solanum tuberosum OX=4113 PE=2... [more]
P348992.1e-26487.40Serine hydroxymethyltransferase, mitochondrial OS=Pisum sativum OX=3888 PE=1 SV=... [more]
Q9SZJ55.8e-26286.85Serine hydroxymethyltransferase 1, mitochondrial OS=Arabidopsis thaliana OX=3702... [more]
P493575.4e-26086.49Serine hydroxymethyltransferase 1, mitochondrial OS=Flaveria pringlei OX=4226 PE... [more]
P493581.9e-25786.10Serine hydroxymethyltransferase 2, mitochondrial OS=Flaveria pringlei OX=4226 PE... [more]
Match NameE-valueIdentityDescription
XP_004143436.10.0100.00serine hydroxymethyltransferase, mitochondrial [Cucumis sativus] >KGN48681.1 hyp... [more]
XP_008440509.10.098.27PREDICTED: serine hydroxymethyltransferase, mitochondrial [Cucumis melo] >KAA003... [more]
XP_038882636.10.096.14serine hydroxymethyltransferase, mitochondrial [Benincasa hispida][more]
XP_022979027.10.094.98serine hydroxymethyltransferase, mitochondrial [Cucurbita maxima] >XP_022979029.... [more]
XP_022949662.10.094.79serine hydroxymethyltransferase, mitochondrial [Cucurbita moschata] >XP_02294966... [more]
Match NameE-valueIdentityDescription
A0A0A0KLP60.0100.00Serine hydroxymethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_6G497310 PE=3 ... [more]
A0A5A7SZ460.098.27Serine hydroxymethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A1S3B1B20.098.27Serine hydroxymethyltransferase OS=Cucumis melo OX=3656 GN=LOC103484914 PE=3 SV=... [more]
A0A6J1IMP00.094.98Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111478793 PE=3... [more]
A0A6J1GCP50.094.79Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111452985 PE... [more]
Match NameE-valueIdentityDescription
AT4G37930.14.1e-26386.85serine transhydroxymethyltransferase 1 [more]
AT5G26780.15.8e-25784.69serine hydroxymethyltransferase 2 [more]
AT5G26780.33.5e-25482.33serine hydroxymethyltransferase 2 [more]
AT5G26780.23.5e-25482.33serine hydroxymethyltransferase 2 [more]
AT4G32520.11.3e-16360.25serine hydroxymethyltransferase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001085Serine hydroxymethyltransferasePIRSFPIRSF000412SHMTcoord: 40..514
e-value: 6.2E-168
score: 556.6
IPR001085Serine hydroxymethyltransferasePANTHERPTHR11680SERINE HYDROXYMETHYLTRANSFERASEcoord: 7..518
IPR001085Serine hydroxymethyltransferaseHAMAPMF_00051SHMTcoord: 56..509
score: 42.233799
IPR001085Serine hydroxymethyltransferaseCDDcd00378SHMTcoord: 61..470
e-value: 0.0
score: 631.93
IPR015422Pyridoxal phosphate-dependent transferase, small domainGENE3D3.90.1150.10Aspartate Aminotransferase, domain 1coord: 61..506
e-value: 1.2E-214
score: 714.7
IPR039429Serine hydroxymethyltransferase-like domainPFAMPF00464SHMTcoord: 58..454
e-value: 2.1E-213
score: 708.2
IPR015421Pyridoxal phosphate-dependent transferase, major domainGENE3D3.40.640.10coord: 86..359
e-value: 1.2E-214
score: 714.7
NoneNo IPR availablePANTHERPTHR11680:SF46SERINE HYDROXYMETHYLTRANSFERASE, MITOCHONDRIALcoord: 7..518
IPR019798Serine hydroxymethyltransferase, pyridoxal phosphate binding sitePROSITEPS00096SHMTcoord: 280..296
IPR015424Pyridoxal phosphate-dependent transferaseSUPERFAMILY53383PLP-dependent transferasescoord: 46..510

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy6G029090.2CsGy6G029090.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046655 folic acid metabolic process
biological_process GO:0019264 glycine biosynthetic process from serine
biological_process GO:0006565 L-serine catabolic process
biological_process GO:0032259 methylation
biological_process GO:0035999 tetrahydrofolate interconversion
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004372 glycine hydroxymethyltransferase activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0070905 serine binding
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0003824 catalytic activity