CsGy6G028030 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy6G028030
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionAuxin-responsive GH3 family protein
LocationGy14Chr6: 27036196 .. 27038823 (+)
RNA-Seq ExpressionCsGy6G028030
SyntenyCsGy6G028030
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTTCTTCCTCTCATTCCTTCTCTCCTCGCATCCTTTCAAACTCGTTTTAATTTTATCAAATAATGGCCGTTGTTGATAATTCTGAATTTTCAACTCCTCTTGGACCGCCTGCCTGCGAGAAAGATGCCAAAGCCCTTCGTTTTATAGAAGAAACCACTACTAATACTGACTCGGTTCAGCAAAGGGTTTTGGCTGAGATTCTCACCCAAAACGCTCACACTGAATATGTCAACCGGTTCCGGCTCAATGGAGCTACCGACCGGGATACCTTTAAATCCAAATTTCCTGTTGTCACTTATGAAGATCTCCAACCTGATATTCAACGTATTGCTAATGGCGATCGCTCTCCTATTTTTTCATCCCATCCCATTTCTGAATTCCTCACCAGGTTTCATTTCGTAATTCCCCCTTTTTTTTGCTATTATTATTTATTAATTAAACTAAATTAACTTAGGGTTAATTATATATGTTTAATAATTTATCCAGTTCTGGGACATCGGCTGGAGAGAGAAAATTGATGCCGACAATTAAAGAAGAAATGGAACGGCGTCAATTACTATACAGCCTTCTCATGCCCATCATGAATCTGTAGGTTTTCTTCTTTTTTTAAATTAAAAAAAAATAATTTGGTGAAAATTATATATAAAAAAAATTTGACAATGAAAAAAAATATATATAATTTTCAGGTATGTGCCGGGATTGGACAAAGGAAAAGGACTCTACTTTTTATTCGTGAAGGCGGAGACTAAGACTCCTGGAGGTTTGGTGGCTCGTCCAGTACTGACTAGTTATTACAAGAGCGATATCTTCAAGACTCGGCATTATGACCCTTTCAATGACTACACTAGCCCTAACGAGGCGGTTTTATGCGCTGATTCTTTTCAAAGCATGTACACTCAAATGTTATGTGGCTTGTTGATGCGTGACCAAGTCCTCCGCGTTGGTGCGGTTTTTGCCTCCGGTCTCCTCCGTGCCATTCGCTTTCTCCAGCTTAACTGGAAACAGCTGGCACACGACATCTCCACCGGAACCCTAAACCCTAAAATCACGGACACTTGTCTTCGTGAATGTATAACCTCCAAATATCTAACAAACCCGAAGCCGGAGCTGGCGGAATTCATCTCAAGTGAATGCTGCACGGAGGAATGGGAAGGGATTATCACGAGAATTTGGCCGAATACTAAATATTTGGATGTGATTGTGACTGGAGCCATGGCTCAGTATATTCCCACCCTCGAGTTTTACAGCGGTGGTTTACCCATGGCTTGCACTATGTATGCTTCTTCCGAGTGCTATTTTGGGGTAAATTTAAACCCAATGTGTAAACCCTCGGATGTGACCTATACCATCATGCCAAACATGGGTTACTTTGAATTTCTCCCCCATGACTCGTCGTCGCGAGCTCCCGCTCTTTCACGTGACTCCCCACCCCGACTTGTTGACCTTGCCGATGTCGAAGTTGGGAAAGAGTACGAGCTTGTTATAACTACTTATGCTGGATTGGTTCGATACCGGGTTGGTGATGTCCTCGAAGTCACTGGTTTCCATAACGCCGCTCCGCAGTTCCGATTTGTGAGGCGGAAGAATGTTCTTTTGAGTATTGACTCTGACAAGACCGATGAGGCCGAGCTCCAGAAAGCGGTCGAGAACGCGTCGTCTCTTCTACGAGAGTTCAACACCACTGTGGTGGAATACACAAGTTATGCAGATACCAAAACAATCCCTGGACATTACGTTATATACTGGGAGCTTCTTGTGAAAGATAATGAAACAATGAATTCTCATCCAACGGATGAGATTTTAAACCAGTGTTGCTTGGCTATTGAAGAAAGTTTGAACTCGGTTTACCGACAAGGCCGAGTTGCGGATAACTCAATTGGACCATTGGAGATTAGGGTGGTGAAAAACGGGACATTTGAAGAGTTAATGGACTATGCGATCTCGAGAGGCGCGTCGATCAACCAATACAAAGCACCACGGTGCGTAAACTTCACACCGATTGTCGAGCTTCTTGACTCTAGGGTAACTTCGTTTCACTTCAGCCCATCAAAGCCGCACTGGACACCAGAACGACGTCGTTGAGGTTGACAACAAAAATAAAAATTTCACATTAAAAAAAAAATTGAAAGCGACCCATATGTATAAAAAGATTATTATGATGATGATGTAGAAGCCAAAGTAGAAGAGGATAGAGTTTGAGTTTGTTGGATTATGCATATGGCTTTTAGAAGTGTCTTTTGATTATTATTATTATTATTGTAGAGTTTTGTGTTTTTTTTTTTTTTATTTCTTAGTGAAAATTAATTTCTAAGTTCATGTGTCATGTCTGTGCAATAAGGTTCAAGTAATTAATGTCTAAAATATGGGCTTCATGATGTAATTGTTCTTTTACAAAATATGTCTGTTGGTCTATTATATATTGAATCTTTTTGTCTTTGGCTTTCTTCTTTATTTTTCTTGCTTTTTAGAGTAAAGAAAGAGTGAAAAGGGGAAAAAAAAGGCAAAGAAAAAGGTTTTGTTGAAAGTGAAAATAATTGCTTTTTATGAAATACATATTTGTTTTTGTTGTTGCTAGCTAGCTGCTAGCTGCTGCTTCCATAAAGCAACCAAACACTGCATATTTTCT

mRNA sequence

CTTTTCTTCCTCTCATTCCTTCTCTCCTCGCATCCTTTCAAACTCGTTTTAATTTTATCAAATAATGGCCGTTGTTGATAATTCTGAATTTTCAACTCCTCTTGGACCGCCTGCCTGCGAGAAAGATGCCAAAGCCCTTCGTTTTATAGAAGAAACCACTACTAATACTGACTCGGTTCAGCAAAGGGTTTTGGCTGAGATTCTCACCCAAAACGCTCACACTGAATATGTCAACCGGTTCCGGCTCAATGGAGCTACCGACCGGGATACCTTTAAATCCAAATTTCCTGTTGTCACTTATGAAGATCTCCAACCTGATATTCAACGTATTGCTAATGGCGATCGCTCTCCTATTTTTTCATCCCATCCCATTTCTGAATTCCTCACCAGTTCTGGGACATCGGCTGGAGAGAGAAAATTGATGCCGACAATTAAAGAAGAAATGGAACGGCGTCAATTACTATACAGCCTTCTCATGCCCATCATGAATCTGTATGTGCCGGGATTGGACAAAGGAAAAGGACTCTACTTTTTATTCGTGAAGGCGGAGACTAAGACTCCTGGAGGTTTGGTGGCTCGTCCAGTACTGACTAGTTATTACAAGAGCGATATCTTCAAGACTCGGCATTATGACCCTTTCAATGACTACACTAGCCCTAACGAGGCGGTTTTATGCGCTGATTCTTTTCAAAGCATGTACACTCAAATGTTATGTGGCTTGTTGATGCGTGACCAAGTCCTCCGCGTTGGTGCGGTTTTTGCCTCCGGTCTCCTCCGTGCCATTCGCTTTCTCCAGCTTAACTGGAAACAGCTGGCACACGACATCTCCACCGGAACCCTAAACCCTAAAATCACGGACACTTGTCTTCGTGAATGTATAACCTCCAAATATCTAACAAACCCGAAGCCGGAGCTGGCGGAATTCATCTCAAGTGAATGCTGCACGGAGGAATGGGAAGGGATTATCACGAGAATTTGGCCGAATACTAAATATTTGGATGTGATTGTGACTGGAGCCATGGCTCAGTATATTCCCACCCTCGAGTTTTACAGCGGTGGTTTACCCATGGCTTGCACTATGTATGCTTCTTCCGAGTGCTATTTTGGGGTAAATTTAAACCCAATGTGTAAACCCTCGGATGTGACCTATACCATCATGCCAAACATGGGTTACTTTGAATTTCTCCCCCATGACTCGTCGTCGCGAGCTCCCGCTCTTTCACGTGACTCCCCACCCCGACTTGTTGACCTTGCCGATGTCGAAGTTGGGAAAGAGTACGAGCTTGTTATAACTACTTATGCTGGATTGGTTCGATACCGGGTTGGTGATGTCCTCGAAGTCACTGGTTTCCATAACGCCGCTCCGCAGTTCCGATTTGTGAGGCGGAAGAATGTTCTTTTGAGTATTGACTCTGACAAGACCGATGAGGCCGAGCTCCAGAAAGCGGTCGAGAACGCGTCGTCTCTTCTACGAGAGTTCAACACCACTGTGGTGGAATACACAAGTTATGCAGATACCAAAACAATCCCTGGACATTACGTTATATACTGGGAGCTTCTTGTGAAAGATAATGAAACAATGAATTCTCATCCAACGGATGAGATTTTAAACCAGTGTTGCTTGGCTATTGAAGAAAGTTTGAACTCGGTTTACCGACAAGGCCGAGTTGCGGATAACTCAATTGGACCATTGGAGATTAGGGTGGTGAAAAACGGGACATTTGAAGAGTTAATGGACTATGCGATCTCGAGAGGCGCGTCGATCAACCAATACAAAGCACCACGGTGCGTAAACTTCACACCGATTGTCGAGCTTCTTGACTCTAGGGTAACTTCGTTTCACTTCAGCCCATCAAAGCCGCACTGGACACCAGAACGACGTCGTTGAGGTTGACAACAAAAATAAAAATTTCACATTAAAAAAAAAATTGAAAGCGACCCATATGTATAAAAAGATTATTATGATGATGATGTAGAAGCCAAAGTAGAAGAGGATAGAGTTTGAGTTTGTTGGATTATGCATATGGCTTTTAGAAGTGTCTTTTGATTATTATTATTATTATTGTAGAGTTTTGTGTTTTTTTTTTTTTTATTTCTTAGTGAAAATTAATTTCTAAGTTCATGTGTCATGTCTGTGCAATAAGGTTCAAGTAATTAATGTCTAAAATATGGGCTTCATGATGTAATTGTTCTTTTACAAAATATGTCTGTTGGTCTATTATATATTGAATCTTTTTGTCTTTGGCTTTCTTCTTTATTTTTCTTGCTTTTTAGAGTAAAGAAAGAGTGAAAAGGGGAAAAAAAAGGCAAAGAAAAAGGTTTTGTTGAAAGTGAAAATAATTGCTTTTTATGAAATACATATTTGTTTTTGTTGTTGCTAGCTAGCTGCTAGCTGCTGCTTCCATAAAGCAACCAAACACTGCATATTTTCT

Coding sequence (CDS)

ATGGCCGTTGTTGATAATTCTGAATTTTCAACTCCTCTTGGACCGCCTGCCTGCGAGAAAGATGCCAAAGCCCTTCGTTTTATAGAAGAAACCACTACTAATACTGACTCGGTTCAGCAAAGGGTTTTGGCTGAGATTCTCACCCAAAACGCTCACACTGAATATGTCAACCGGTTCCGGCTCAATGGAGCTACCGACCGGGATACCTTTAAATCCAAATTTCCTGTTGTCACTTATGAAGATCTCCAACCTGATATTCAACGTATTGCTAATGGCGATCGCTCTCCTATTTTTTCATCCCATCCCATTTCTGAATTCCTCACCAGTTCTGGGACATCGGCTGGAGAGAGAAAATTGATGCCGACAATTAAAGAAGAAATGGAACGGCGTCAATTACTATACAGCCTTCTCATGCCCATCATGAATCTGTATGTGCCGGGATTGGACAAAGGAAAAGGACTCTACTTTTTATTCGTGAAGGCGGAGACTAAGACTCCTGGAGGTTTGGTGGCTCGTCCAGTACTGACTAGTTATTACAAGAGCGATATCTTCAAGACTCGGCATTATGACCCTTTCAATGACTACACTAGCCCTAACGAGGCGGTTTTATGCGCTGATTCTTTTCAAAGCATGTACACTCAAATGTTATGTGGCTTGTTGATGCGTGACCAAGTCCTCCGCGTTGGTGCGGTTTTTGCCTCCGGTCTCCTCCGTGCCATTCGCTTTCTCCAGCTTAACTGGAAACAGCTGGCACACGACATCTCCACCGGAACCCTAAACCCTAAAATCACGGACACTTGTCTTCGTGAATGTATAACCTCCAAATATCTAACAAACCCGAAGCCGGAGCTGGCGGAATTCATCTCAAGTGAATGCTGCACGGAGGAATGGGAAGGGATTATCACGAGAATTTGGCCGAATACTAAATATTTGGATGTGATTGTGACTGGAGCCATGGCTCAGTATATTCCCACCCTCGAGTTTTACAGCGGTGGTTTACCCATGGCTTGCACTATGTATGCTTCTTCCGAGTGCTATTTTGGGGTAAATTTAAACCCAATGTGTAAACCCTCGGATGTGACCTATACCATCATGCCAAACATGGGTTACTTTGAATTTCTCCCCCATGACTCGTCGTCGCGAGCTCCCGCTCTTTCACGTGACTCCCCACCCCGACTTGTTGACCTTGCCGATGTCGAAGTTGGGAAAGAGTACGAGCTTGTTATAACTACTTATGCTGGATTGGTTCGATACCGGGTTGGTGATGTCCTCGAAGTCACTGGTTTCCATAACGCCGCTCCGCAGTTCCGATTTGTGAGGCGGAAGAATGTTCTTTTGAGTATTGACTCTGACAAGACCGATGAGGCCGAGCTCCAGAAAGCGGTCGAGAACGCGTCGTCTCTTCTACGAGAGTTCAACACCACTGTGGTGGAATACACAAGTTATGCAGATACCAAAACAATCCCTGGACATTACGTTATATACTGGGAGCTTCTTGTGAAAGATAATGAAACAATGAATTCTCATCCAACGGATGAGATTTTAAACCAGTGTTGCTTGGCTATTGAAGAAAGTTTGAACTCGGTTTACCGACAAGGCCGAGTTGCGGATAACTCAATTGGACCATTGGAGATTAGGGTGGTGAAAAACGGGACATTTGAAGAGTTAATGGACTATGCGATCTCGAGAGGCGCGTCGATCAACCAATACAAAGCACCACGGTGCGTAAACTTCACACCGATTGTCGAGCTTCTTGACTCTAGGGTAACTTCGTTTCACTTCAGCCCATCAAAGCCGCACTGGACACCAGAACGACGTCGTTGA

Protein sequence

MAVVDNSEFSTPLGPPACEKDAKALRFIEETTTNTDSVQQRVLAEILTQNAHTEYVNRFRLNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFKTRHYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIRFLQLNWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPKPELAEFISSECCTEEWEGIITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMGYFEFLPHDSSSRAPALSRDSPPRLVDLADVEVGKEYELVITTYAGLVRYRVGDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYTSYADTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSFHFSPSKPHWTPERRR*
Homology
BLAST of CsGy6G028030 vs. ExPASy Swiss-Prot
Match: O82333 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=3702 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 938.7 bits (2425), Expect = 3.2e-272
Identity = 462/603 (76.62%), Postives = 516/603 (85.57%), Query Frame = 0

Query: 6   NSEFSTPLGPPACEKDAKALRFIEETTTNTDSVQQRVLAEILTQNAHTEYVNRFRLNGAT 65
           +S  S+PLGPPACEKDAKALRFIEE T N D+VQ+ +LAEIL +NA TEY+ RF L GAT
Sbjct: 4   DSNLSSPLGPPACEKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGAT 63

Query: 66  DRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKE 125
           DRDTFK+K PV+TYEDLQP+IQRIA+GDRSPI S+HPISEFLTSSGTSAGERKLMPTIKE
Sbjct: 64  DRDTFKTKIPVITYEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKE 123

Query: 126 EMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFK 185
           E++RRQLLYSLLMP+MNLYVPGLDKGKG+YFLFVK+ETKTPGGL ARPVLTSYYKS+ F+
Sbjct: 124 ELDRRQLLYSLLMPVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFR 183

Query: 186 TRHYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIRFLQL 245
           +R YDP+N YTSPNEA+LC DSFQSMYTQMLCGLL R  VLRVGAVFASGLLRAIRFLQL
Sbjct: 184 SRPYDPYNVYTSPNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQL 243

Query: 246 NWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPKPELAEFISSECCTEEWEGIITRIW 305
           +W + AHDI  G L+ +ITD  +R+C++   +  P P LAEFI  EC ++ WE IITRIW
Sbjct: 244 HWSRFAHDIELGCLDSEITDPSIRQCMSG--ILKPDPVLAEFIRRECKSDNWEKIITRIW 303

Query: 306 PNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIM 365
           PNTKYLDVIVTGAMAQYIPTLE+YSGGLPMACTMYASSECYFG+NLNPM KPS+V+YTIM
Sbjct: 304 PNTKYLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIM 363

Query: 366 PNMGYFEFLPHDSSSRAPALSRDSPPRLVDLADVEVGKEYELVITTYAGLVRYRVGDVLE 425
           PNM YFEF+P   +            + V+L DV +GKEYELV+TTYAGL RYRVGD+L 
Sbjct: 364 PNMAYFEFIPLGGT------------KAVELVDVNIGKEYELVVTTYAGLCRYRVGDILR 423

Query: 426 VTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLL-REFNTTVVEYTSYAD 485
           VTGFHN+APQF FVRRKNVLLSIDSDKTDE+ELQKAVENASS+L  E  + V EYTSYAD
Sbjct: 424 VTGFHNSAPQFHFVRRKNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYAD 483

Query: 486 TKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSIGPLE 545
           T TIPGHYV+YWELLV+D     SH   E L +CCL +EESLNSVYRQ RVADNS+GPLE
Sbjct: 484 TSTIPGHYVLYWELLVRDGARQPSH---ETLTRCCLGMEESLNSVYRQSRVADNSVGPLE 543

Query: 546 IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSFHFSPSKPHWTPE 605
           IRVV+NGTFEELMDYAISRGASINQYK PRCVNFTPIVELLDSRV S HFSPS PHWTPE
Sbjct: 544 IRVVRNGTFEELMDYAISRGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPE 589

Query: 606 RRR 608
           RRR
Sbjct: 604 RRR 589

BLAST of CsGy6G028030 vs. ExPASy Swiss-Prot
Match: O22190 (Indole-3-acetic acid-amido synthetase GH3.3 OS=Arabidopsis thaliana OX=3702 GN=GH3.3 PE=1 SV=1)

HSP 1 Score: 896.3 bits (2315), Expect = 1.8e-259
Identity = 449/603 (74.46%), Postives = 508/603 (84.25%), Query Frame = 0

Query: 6   NSEFSTPLGPPACEKDAKALRFIEETTTNTDSVQQRVLAEILTQNAHTEYVNRFRLNGAT 65
           +S   +P+      KD KALRFIEE T N D VQ++V+ EIL++N+ TEY+ RF L G T
Sbjct: 4   DSALRSPMMHSPSTKDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLKGFT 63

Query: 66  DRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKE 125
           DR TFK+K PVV Y+DL+P+IQRIANGDRS I SS+PI+EFLTSSGTSAGERKLMPTI E
Sbjct: 64  DRKTFKTKVPVVIYDDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPTIDE 123

Query: 126 EMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFK 185
           +M+RRQLLYSLLMP+MNLYVPGLDKGK LYFLFVK E+KTPGGL ARPVLTSYYKS+ FK
Sbjct: 124 DMDRRQLLYSLLMPVMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSEQFK 183

Query: 186 TRHYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIRFLQL 245
            R  DP+N YTSPNEA+LC DS QSMYTQMLCGLLMR +VLR+GAVFASGLLRAI FLQ 
Sbjct: 184 RRPNDPYNVYTSPNEAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGFLQT 243

Query: 246 NWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPKPELAEFISSECCTE-EWEGIITRI 305
           NWK+LA DISTGTL+ +I+D  ++E + SK LT P  ELA+FI+S C  +  WEGIIT+I
Sbjct: 244 NWKELADDISTGTLSSRISDPAIKESM-SKILTKPDQELADFITSVCGQDNSWEGIITKI 303

Query: 306 WPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTI 365
           WPNTKYLDVIVTGAMAQYIP LE+YSGGLPMACTMYASSE YFG+NL PMCKPS+V+YTI
Sbjct: 304 WPNTKYLDVIVTGAMAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTI 363

Query: 366 MPNMGYFEFLPHDSSSRAPALSRDSPPRLVDLADVEVGKEYELVITTYAGLVRYRVGDVL 425
           MPNM YFEFLPH       +        LV+LADVEVGKEYELVITTYAGL RYRVGD+L
Sbjct: 364 MPNMAYFEFLPHHEVPTEKS-------ELVELADVEVGKEYELVITTYAGLNRYRVGDIL 423

Query: 426 EVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYTSYAD 485
           +VTGF+N+APQF+FVRRKNVLLSI+SDKTDEAELQ AVENAS LL E  T V+EYTSYA+
Sbjct: 424 QVTGFYNSAPQFKFVRRKNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAE 483

Query: 486 TKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSIGPLE 545
           TKTIPGHYVIYWELLVKD     + P DE++ +CCL +EESLNSVYRQ RVAD SIGPLE
Sbjct: 484 TKTIPGHYVIYWELLVKD---QTNPPNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLE 543

Query: 546 IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSFHFSPSKPHWTPE 605
           IRVVKNGTFEELMDYAISRGASINQYK PRCV+FTPI+ELLDSRV S HFSP+ PHW+PE
Sbjct: 544 IRVVKNGTFEELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSTHFSPALPHWSPE 595

Query: 606 RRR 608
           RRR
Sbjct: 604 RRR 595

BLAST of CsGy6G028030 vs. ExPASy Swiss-Prot
Match: A3BLS0 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=39946 GN=GH3.8 PE=1 SV=2)

HSP 1 Score: 880.9 bits (2275), Expect = 8.0e-255
Identity = 447/612 (73.04%), Postives = 503/612 (82.19%), Query Frame = 0

Query: 1   MAVV-DNSEFSTPLGPPAC----EKDAKALRFIEETTTNTDSVQQRVLAEILTQNAHTEY 60
           MAV+ D S   T L  PA     E D + LRFI+E TTN D+VQ+RVL EIL +NA TEY
Sbjct: 1   MAVMTDVSTTGTALRTPAAGAVKEGDVEKLRFIDEMTTNVDAVQERVLGEILGRNAGTEY 60

Query: 61  VNRFRLNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAG 120
           + +  L+GATDR  F++K PVV+Y+DLQP IQRIANGDRSPI S+HP+SEFLTSSGTSAG
Sbjct: 61  LTKCGLDGATDRAAFRAKVPVVSYDDLQPYIQRIANGDRSPILSTHPVSEFLTSSGTSAG 120

Query: 121 ERKLMPTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVL 180
           ERKLMPTI +E++RRQLLYSLLMP+MNLYVPGLDKGKGLYFLFVK+ETKTPGGL ARPVL
Sbjct: 121 ERKLMPTIMDELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETKTPGGLTARPVL 180

Query: 181 TSYYKSDIFKTRHYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASG 240
           TSYYKSD FK R YDP+++YTSP  A+LCAD+FQSMY QM+CGL  R+ VLR+GAVFASG
Sbjct: 181 TSYYKSDHFKNRPYDPYHNYTSPTAAILCADAFQSMYAQMVCGLCQRNDVLRLGAVFASG 240

Query: 241 LLRAIRFLQLNWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPKPELAEFISSECCTE 300
           LLRAIRFLQLNW+QLA DI +G L P++TD  +RE + +  L  P PELA+ I +EC   
Sbjct: 241 LLRAIRFLQLNWEQLADDIESGELTPRVTDPSVREAVAAILL--PDPELAKLIRAECSKG 300

Query: 301 EWEGIITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMC 360
           +W GIITR+WPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFG+NL PMC
Sbjct: 301 DWAGIITRVWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGLNLRPMC 360

Query: 361 KPSDVTYTIMPNMGYFEFLPHDSSSRAPALSRDSPPRLVDLADVEVGKEYELVITTYAGL 420
            PS+V+YTIMPNMGYFEFLP D +  A         +LVDLA VEVG+EYELVITTYAGL
Sbjct: 361 DPSEVSYTIMPNMGYFEFLPVDETGAASG----DATQLVDLARVEVGREYELVITTYAGL 420

Query: 421 VRYRVGDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTT 480
            RYRVGDVL VTGFHNAAPQFRFVRRKNVLLSI+SDKTDEAELQ+AVE AS+LLR    +
Sbjct: 421 NRYRVGDVLRVTGFHNAAPQFRFVRRKNVLLSIESDKTDEAELQRAVERASALLRPHGAS 480

Query: 481 VVEYTSYADTKTIPGHYVIYWELLVKD-NETMNSHPTDEILNQCCLAIEESLNSVYRQGR 540
           VVEYTS A TK IPGHYVIYWELL K    T+    T   L +CCL +EE+LN+VYRQ R
Sbjct: 481 VVEYTSQACTKRIPGHYVIYWELLTKGAGATVVDADT---LGRCCLEMEEALNTVYRQSR 540

Query: 541 VADNSIGPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSFHF 600
           VAD SIGPLEIRVV+ GTFEELMDYAISRGASINQYK PRCV F PIVELLDSRV S HF
Sbjct: 541 VADGSIGPLEIRVVRPGTFEELMDYAISRGASINQYKVPRCVTFPPIVELLDSRVVSSHF 600

Query: 601 SPSKPHWTPERR 607
           SP+ PHWTP RR
Sbjct: 601 SPALPHWTPARR 603

BLAST of CsGy6G028030 vs. ExPASy Swiss-Prot
Match: Q0D4Z6 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.8 PE=1 SV=1)

HSP 1 Score: 880.9 bits (2275), Expect = 8.0e-255
Identity = 447/612 (73.04%), Postives = 503/612 (82.19%), Query Frame = 0

Query: 1   MAVV-DNSEFSTPLGPPAC----EKDAKALRFIEETTTNTDSVQQRVLAEILTQNAHTEY 60
           MAV+ D S   T L  PA     E D + LRFI+E TTN D+VQ+RVL EIL +NA TEY
Sbjct: 1   MAVMTDVSTTGTALRTPAAGAVKEGDVEKLRFIDEMTTNVDAVQERVLGEILGRNAGTEY 60

Query: 61  VNRFRLNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAG 120
           + +  L+GATDR  F++K PVV+Y+DLQP IQRIANGDRSPI S+HP+SEFLTSSGTSAG
Sbjct: 61  LTKCGLDGATDRAAFRAKVPVVSYDDLQPYIQRIANGDRSPILSTHPVSEFLTSSGTSAG 120

Query: 121 ERKLMPTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVL 180
           ERKLMPTI +E++RRQLLYSLLMP+MNLYVPGLDKGKGLYFLFVK+ETKTPGGL ARPVL
Sbjct: 121 ERKLMPTIMDELDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSETKTPGGLTARPVL 180

Query: 181 TSYYKSDIFKTRHYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASG 240
           TSYYKSD FK R YDP+++YTSP  A+LCAD+FQSMY QM+CGL  R+ VLR+GAVFASG
Sbjct: 181 TSYYKSDHFKNRPYDPYHNYTSPTAAILCADAFQSMYAQMVCGLCQRNDVLRLGAVFASG 240

Query: 241 LLRAIRFLQLNWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPKPELAEFISSECCTE 300
           LLRAIRFLQLNW+QLA DI +G L P++TD  +RE + +  L  P PELA+ I +EC   
Sbjct: 241 LLRAIRFLQLNWEQLADDIESGELTPRVTDPSVREAVAAILL--PDPELAKLIRAECSKG 300

Query: 301 EWEGIITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMC 360
           +W GIITR+WPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFG+NL PMC
Sbjct: 301 DWAGIITRVWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGLNLRPMC 360

Query: 361 KPSDVTYTIMPNMGYFEFLPHDSSSRAPALSRDSPPRLVDLADVEVGKEYELVITTYAGL 420
            PS+V+YTIMPNMGYFEFLP D +  A         +LVDLA VEVG+EYELVITTYAGL
Sbjct: 361 DPSEVSYTIMPNMGYFEFLPVDETGAASG----DATQLVDLARVEVGREYELVITTYAGL 420

Query: 421 VRYRVGDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTT 480
            RYRVGDVL VTGFHNAAPQFRFVRRKNVLLSI+SDKTDEAELQ+AVE AS+LLR    +
Sbjct: 421 NRYRVGDVLRVTGFHNAAPQFRFVRRKNVLLSIESDKTDEAELQRAVERASALLRPHGAS 480

Query: 481 VVEYTSYADTKTIPGHYVIYWELLVKD-NETMNSHPTDEILNQCCLAIEESLNSVYRQGR 540
           VVEYTS A TK IPGHYVIYWELL K    T+    T   L +CCL +EE+LN+VYRQ R
Sbjct: 481 VVEYTSQACTKRIPGHYVIYWELLTKGAGATVVDADT---LGRCCLEMEEALNTVYRQSR 540

Query: 541 VADNSIGPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSFHF 600
           VAD SIGPLEIRVV+ GTFEELMDYAISRGASINQYK PRCV F PIVELLDSRV S HF
Sbjct: 541 VADGSIGPLEIRVVRPGTFEELMDYAISRGASINQYKVPRCVTFPPIVELLDSRVVSSHF 600

Query: 601 SPSKPHWTPERR 607
           SP+ PHWTP RR
Sbjct: 601 SPALPHWTPARR 603

BLAST of CsGy6G028030 vs. ExPASy Swiss-Prot
Match: P0C0M2 (Probable indole-3-acetic acid-amido synthetase GH3.2 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.2 PE=2 SV=1)

HSP 1 Score: 867.8 bits (2241), Expect = 7.0e-251
Identity = 423/594 (71.21%), Postives = 502/594 (84.51%), Query Frame = 0

Query: 17  ACEKDAKALRFIEETTTNTDSVQQRVLAEILTQNAHTEYVNRFRLNGATDRDTFKSKFPV 76
           ACE+DA+ L FIEE T   D+VQ+RVLA IL +N   EY+ R  + G TDR+ FK++ PV
Sbjct: 25  ACERDAEKLEFIEEMTRGFDAVQERVLAAILARNNGAEYLRRHGMEGRTDREAFKARVPV 84

Query: 77  VTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQLLYSL 136
           VTYEDL+P+I+RIANGDRS I SSHPI+EFLTSSGTSAGERKLMPTI++E++RRQ+LYSL
Sbjct: 85  VTYEDLRPEIERIANGDRSNIISSHPITEFLTSSGTSAGERKLMPTIEDELDRRQMLYSL 144

Query: 137 LMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFKTRHYDPFNDYT 196
           LMP+MNLYVPGLDKGKGLYFLF+K+ETKTPGGL ARPVLTSYYKSD FK R +DP+N YT
Sbjct: 145 LMPVMNLYVPGLDKGKGLYFLFIKSETKTPGGLPARPVLTSYYKSDHFKHRPFDPYNVYT 204

Query: 197 SPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIRFLQLNWKQLAHDIST 256
           SP  A+LC D+FQSMY QMLCGL+ R +VLRVGAVFASGLLRAIRFLQL+W++LAHDI T
Sbjct: 205 SPTAAILCTDAFQSMYAQMLCGLVARAEVLRVGAVFASGLLRAIRFLQLHWRELAHDIRT 264

Query: 257 GTLNPKITDTCLRECITSKYLTNPKPELAEFISSECCTEEWEGIITRIWPNTKYLDVIVT 316
           GTL+ K+T+  +R+ + ++ L  P  ELA F+ +EC  ++WEGIITR+WPNTKYLDVIVT
Sbjct: 265 GTLSAKVTEPSIRDAV-AEVLAAPDAELAAFVEAECGKDKWEGIITRMWPNTKYLDVIVT 324

Query: 317 GAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMGYFEFLPH 376
           GAMAQYIPTL+FYSGGLPMACTMYASSECYFG+NL PMC PS+V+YTIMPNMGYFE +PH
Sbjct: 325 GAMAQYIPTLKFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFELMPH 384

Query: 377 DSSSRAPALSRDS-PPRLVDLADVEVGKEYELVITTYAGLVRYRVGDVLEVTGFHNAAPQ 436
           D    AP L RD+ PPRLVDLAD EVG+EYELVITTYAGL RYRVGD+L+VTGFHNAAPQ
Sbjct: 385 DPD--APPLPRDAPPPRLVDLADAEVGREYELVITTYAGLCRYRVGDILQVTGFHNAAPQ 444

Query: 437 FRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYTSYADTKTIPGHYVIY 496
           FRFVRRKNVLLSIDSDKTDEAELQ AVE AS+LL  +  ++VEYTS AD  TIPGHYV+Y
Sbjct: 445 FRFVRRKNVLLSIDSDKTDEAELQAAVERASALLSPYGASIVEYTSQADATTIPGHYVVY 504

Query: 497 WELLVKDNETMNSHPTDE---ILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVKNGT 556
           WEL+V++         +E   +  +CCL +EE+LN+VYRQGR  + +IGPLEIRVV+ GT
Sbjct: 505 WELMVREGGAWPPPAEEEGRGVFERCCLEMEEALNAVYRQGRNGE-AIGPLEIRVVRAGT 564

Query: 557 FEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSFHFSPSKPHWTPERR 607
           FEE+MDYAISRGASINQYKAPRCV+F PI+ELL+SRV S HFSP+ P ++P ++
Sbjct: 565 FEEVMDYAISRGASINQYKAPRCVSFGPIIELLNSRVISKHFSPACPKYSPHKK 614

BLAST of CsGy6G028030 vs. NCBI nr
Match: XP_004141893.1 (probable indole-3-acetic acid-amido synthetase GH3.1 [Cucumis sativus] >KGN48572.1 hypothetical protein Csa_003850 [Cucumis sativus])

HSP 1 Score: 1233 bits (3190), Expect = 0.0
Identity = 607/607 (100.00%), Postives = 607/607 (100.00%), Query Frame = 0

Query: 1   MAVVDNSEFSTPLGPPACEKDAKALRFIEETTTNTDSVQQRVLAEILTQNAHTEYVNRFR 60
           MAVVDNSEFSTPLGPPACEKDAKALRFIEETTTNTDSVQQRVLAEILTQNAHTEYVNRFR
Sbjct: 1   MAVVDNSEFSTPLGPPACEKDAKALRFIEETTTNTDSVQQRVLAEILTQNAHTEYVNRFR 60

Query: 61  LNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM 120
           LNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM
Sbjct: 61  LNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM 120

Query: 121 PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK 180
           PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK
Sbjct: 121 PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK 180

Query: 181 SDIFKTRHYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAI 240
           SDIFKTRHYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAI
Sbjct: 181 SDIFKTRHYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAI 240

Query: 241 RFLQLNWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPKPELAEFISSECCTEEWEGI 300
           RFLQLNWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPKPELAEFISSECCTEEWEGI
Sbjct: 241 RFLQLNWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPKPELAEFISSECCTEEWEGI 300

Query: 301 ITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDV 360
           ITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDV
Sbjct: 301 ITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDV 360

Query: 361 TYTIMPNMGYFEFLPHDSSSRAPALSRDSPPRLVDLADVEVGKEYELVITTYAGLVRYRV 420
           TYTIMPNMGYFEFLPHDSSSRAPALSRDSPPRLVDLADVEVGKEYELVITTYAGLVRYRV
Sbjct: 361 TYTIMPNMGYFEFLPHDSSSRAPALSRDSPPRLVDLADVEVGKEYELVITTYAGLVRYRV 420

Query: 421 GDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYT 480
           GDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYT
Sbjct: 421 GDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYT 480

Query: 481 SYADTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSI 540
           SYADTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSI
Sbjct: 481 SYADTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSI 540

Query: 541 GPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSFHFSPSKPH 600
           GPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSFHFSPSKPH
Sbjct: 541 GPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSFHFSPSKPH 600

Query: 601 WTPERRR 607
           WTPERRR
Sbjct: 601 WTPERRR 607

BLAST of CsGy6G028030 vs. NCBI nr
Match: XP_008440335.1 (PREDICTED: probable indole-3-acetic acid-amido synthetase GH3.1 [Cucumis melo] >TYK12848.1 putative indole-3-acetic acid-amido synthetase GH3.1 [Cucumis melo var. makuwa])

HSP 1 Score: 1206 bits (3120), Expect = 0.0
Identity = 595/607 (98.02%), Postives = 599/607 (98.68%), Query Frame = 0

Query: 1   MAVVDNSEFSTPLGPPACEKDAKALRFIEETTTNTDSVQQRVLAEILTQNAHTEYVNRFR 60
           MAVVDNSEFS+PLGPPACEKDAKALRFIEETTTN D VQQRVLAEILTQNAHTEYVNRFR
Sbjct: 1   MAVVDNSEFSSPLGPPACEKDAKALRFIEETTTNADLVQQRVLAEILTQNAHTEYVNRFR 60

Query: 61  LNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM 120
           LNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM
Sbjct: 61  LNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM 120

Query: 121 PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK 180
           PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK
Sbjct: 121 PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK 180

Query: 181 SDIFKTRHYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAI 240
           SDIFKTR YDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAI
Sbjct: 181 SDIFKTRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAI 240

Query: 241 RFLQLNWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPKPELAEFISSECCTEEWEGI 300
           RFLQLNWKQLAHDISTGTLNPKITDTCLRECITSKYLTNP PELAEFIS+EC TEEWEGI
Sbjct: 241 RFLQLNWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPNPELAEFISNECYTEEWEGI 300

Query: 301 ITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDV 360
           ITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDV
Sbjct: 301 ITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDV 360

Query: 361 TYTIMPNMGYFEFLPHDSSSRAPALSRDSPPRLVDLADVEVGKEYELVITTYAGLVRYRV 420
           TYTIMPNMGYFEFLPHDSSSRA ALSRDSPPRLVDLADVEVGKEYELVITTYAGL RYRV
Sbjct: 361 TYTIMPNMGYFEFLPHDSSSRASALSRDSPPRLVDLADVEVGKEYELVITTYAGLCRYRV 420

Query: 421 GDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYT 480
           GDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLR+FNTTVVEYT
Sbjct: 421 GDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLRDFNTTVVEYT 480

Query: 481 SYADTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSI 540
           SYADTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSI
Sbjct: 481 SYADTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSI 540

Query: 541 GPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSFHFSPSKPH 600
           GPLEIRVV+NGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTS HFSPSKPH
Sbjct: 541 GPLEIRVVRNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPH 600

Query: 601 WTPERRR 607
           WTPERRR
Sbjct: 601 WTPERRR 607

BLAST of CsGy6G028030 vs. NCBI nr
Match: XP_038881175.1 (probable indole-3-acetic acid-amido synthetase GH3.1 [Benincasa hispida])

HSP 1 Score: 1181 bits (3054), Expect = 0.0
Identity = 578/607 (95.22%), Postives = 592/607 (97.53%), Query Frame = 0

Query: 1   MAVVDNSEFSTPLGPPACEKDAKALRFIEETTTNTDSVQQRVLAEILTQNAHTEYVNRFR 60
           MAVVDN +FS+PLGPPACEKDAKALRFI+ETT N DSVQQRVLAEILTQNAHTEYVNRFR
Sbjct: 1   MAVVDNPQFSSPLGPPACEKDAKALRFIQETTANADSVQQRVLAEILTQNAHTEYVNRFR 60

Query: 61  LNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM 120
           LNGATDRDTFKSK PVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM
Sbjct: 61  LNGATDRDTFKSKLPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM 120

Query: 121 PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK 180
           PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK
Sbjct: 121 PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK 180

Query: 181 SDIFKTRHYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAI 240
           SDIFKTR YDP+NDYTSPNEAVLCADSFQSMYTQMLCGLLMR QVLRVGAVFASGLLRAI
Sbjct: 181 SDIFKTRPYDPYNDYTSPNEAVLCADSFQSMYTQMLCGLLMRGQVLRVGAVFASGLLRAI 240

Query: 241 RFLQLNWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPKPELAEFISSECCTEEWEGI 300
           RFLQLNWKQLAHDI+TGTLNPKITD+CLRECIT+KYLTNP PELAEFIS EC TEEWEGI
Sbjct: 241 RFLQLNWKQLAHDIATGTLNPKITDSCLRECITTKYLTNPNPELAEFISKECSTEEWEGI 300

Query: 301 ITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDV 360
           ITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKP +V
Sbjct: 301 ITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPDEV 360

Query: 361 TYTIMPNMGYFEFLPHDSSSRAPALSRDSPPRLVDLADVEVGKEYELVITTYAGLVRYRV 420
           TYTIMPNMGYFEFLPHDS S+APALSRDSPPRLVDLADVEVGKEYELVITTYAGL RYRV
Sbjct: 361 TYTIMPNMGYFEFLPHDSLSQAPALSRDSPPRLVDLADVEVGKEYELVITTYAGLCRYRV 420

Query: 421 GDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYT 480
           GDVL+VTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKA+E+ASSLLREFNTTVVEYT
Sbjct: 421 GDVLKVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAIESASSLLREFNTTVVEYT 480

Query: 481 SYADTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSI 540
           SYADTKTIPGHYVIYWELLVKDNE MN HPTDEILNQCCLAIEE+LNSVYRQGRVADNSI
Sbjct: 481 SYADTKTIPGHYVIYWELLVKDNEAMNCHPTDEILNQCCLAIEENLNSVYRQGRVADNSI 540

Query: 541 GPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSFHFSPSKPH 600
           GPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTS HFSP+KPH
Sbjct: 541 GPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPAKPH 600

Query: 601 WTPERRR 607
           WTPERRR
Sbjct: 601 WTPERRR 607

BLAST of CsGy6G028030 vs. NCBI nr
Match: KAG6604151.1 (putative indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1123 bits (2906), Expect = 0.0
Identity = 562/607 (92.59%), Postives = 578/607 (95.22%), Query Frame = 0

Query: 1   MAVVDNSEFSTPLGPPACEKDAKALRFIEETTTNTDSVQQRVLAEILTQNAHTEYVNRFR 60
           MAV  +S+ S+PL PP  +KDAKALRFIEE TTNTD+VQQRVLAEIL++NA+TEYVNRFR
Sbjct: 1   MAV--DSQLSSPLPPPPSDKDAKALRFIEEVTTNTDAVQQRVLAEILSRNANTEYVNRFR 60

Query: 61  LNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM 120
           LNGATDRDT+KSK PVVTYEDLQPDIQRIANGDRSPIFSS PISEFLTSSGTSAGERKLM
Sbjct: 61  LNGATDRDTYKSKIPVVTYEDLQPDIQRIANGDRSPIFSSTPISEFLTSSGTSAGERKLM 120

Query: 121 PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK 180
           PTI+EEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK
Sbjct: 121 PTIQEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK 180

Query: 181 SDIFKTRHYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAI 240
           SDIFKTR YDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMR+QVLRVGAVFASGLLRAI
Sbjct: 181 SDIFKTRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAI 240

Query: 241 RFLQLNWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPKPELAEFISSECCTEEWEGI 300
           RFLQLNWKQLAHDISTGTLNPKITD CLRECI SKYLTNP PE+AEFIS EC TEEWEGI
Sbjct: 241 RFLQLNWKQLAHDISTGTLNPKITDPCLRECIVSKYLTNPNPEVAEFISKECSTEEWEGI 300

Query: 301 ITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDV 360
           ITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNL PMCKPS+V
Sbjct: 301 ITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEV 360

Query: 361 TYTIMPNMGYFEFLPHDSSSRAPALSRDSPPRLVDLADVEVGKEYELVITTYAGLVRYRV 420
           TYTIMPNMGYFEFLPHD+SS  P LSRDSPPRLVDLADVEVGKEYELVITTYAGL RYRV
Sbjct: 361 TYTIMPNMGYFEFLPHDTSS-PPVLSRDSPPRLVDLADVEVGKEYELVITTYAGLRRYRV 420

Query: 421 GDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYT 480
           GDVLEVTGFHNAAPQFRFVRRKNVLLSID+DKTDEAELQKAVENASSLLRE NT+VVEYT
Sbjct: 421 GDVLEVTGFHNAAPQFRFVRRKNVLLSIDADKTDEAELQKAVENASSLLRELNTSVVEYT 480

Query: 481 SYADTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSI 540
           SYADTKTIPGHYVIYWELLVKD E  NS P  EILNQCCLAIEESLNSVYRQGRVADNSI
Sbjct: 481 SYADTKTIPGHYVIYWELLVKD-EAANSPPPAEILNQCCLAIEESLNSVYRQGRVADNSI 540

Query: 541 GPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSFHFSPSKPH 600
           GPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRV S HFSPSKPH
Sbjct: 541 GPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVASVHFSPSKPH 600

Query: 601 WTPERRR 607
           WTPERRR
Sbjct: 601 WTPERRR 603

BLAST of CsGy6G028030 vs. NCBI nr
Match: XP_023543141.1 (probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1123 bits (2906), Expect = 0.0
Identity = 562/607 (92.59%), Postives = 578/607 (95.22%), Query Frame = 0

Query: 1   MAVVDNSEFSTPLGPPACEKDAKALRFIEETTTNTDSVQQRVLAEILTQNAHTEYVNRFR 60
           MAV  +S+ S+PL PP  +KDAKALRFI+E TTNTD+VQQRVLAEIL++NA+TEYVNRFR
Sbjct: 1   MAV--DSQLSSPLPPPPSDKDAKALRFIQEVTTNTDAVQQRVLAEILSRNANTEYVNRFR 60

Query: 61  LNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM 120
           LNGATDRDT+KSK PVVTYEDLQPDIQRIANGDRSPIFSS PISEFLTSSGTSAGERKLM
Sbjct: 61  LNGATDRDTYKSKIPVVTYEDLQPDIQRIANGDRSPIFSSTPISEFLTSSGTSAGERKLM 120

Query: 121 PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK 180
           PTI+EEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK
Sbjct: 121 PTIQEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK 180

Query: 181 SDIFKTRHYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAI 240
           SDIFKTR YDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMR+QVLRVGAVFASGLLRAI
Sbjct: 181 SDIFKTRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAI 240

Query: 241 RFLQLNWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPKPELAEFISSECCTEEWEGI 300
           RFLQLNWKQLAHDISTGTLNPKITD CLRECI SKYLTNP PE+AEFIS EC TEEWEGI
Sbjct: 241 RFLQLNWKQLAHDISTGTLNPKITDPCLRECIVSKYLTNPNPEVAEFISKECSTEEWEGI 300

Query: 301 ITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDV 360
           ITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNL PMCKPS+V
Sbjct: 301 ITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEV 360

Query: 361 TYTIMPNMGYFEFLPHDSSSRAPALSRDSPPRLVDLADVEVGKEYELVITTYAGLVRYRV 420
           TYTIMPNMGYFEFLPHD+SS  P LSRDSPPRLVDLADVEVGKEYELVITTYAGL RYRV
Sbjct: 361 TYTIMPNMGYFEFLPHDTSS-PPVLSRDSPPRLVDLADVEVGKEYELVITTYAGLRRYRV 420

Query: 421 GDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYT 480
           GDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLRE NT+VVEYT
Sbjct: 421 GDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLRELNTSVVEYT 480

Query: 481 SYADTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSI 540
           SYADTKTIPGHYVIYWELLVKD E  NS P  EILNQCCLAIEESLNSVYRQGRVADNSI
Sbjct: 481 SYADTKTIPGHYVIYWELLVKD-EAANSPPPAEILNQCCLAIEESLNSVYRQGRVADNSI 540

Query: 541 GPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSFHFSPSKPH 600
           GPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRV S HFSPSKPH
Sbjct: 541 GPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVASVHFSPSKPH 600

Query: 601 WTPERRR 607
           WTPERRR
Sbjct: 601 WTPERRR 603

BLAST of CsGy6G028030 vs. ExPASy TrEMBL
Match: A0A0A0KJM5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G493310 PE=3 SV=1)

HSP 1 Score: 1233 bits (3190), Expect = 0.0
Identity = 607/607 (100.00%), Postives = 607/607 (100.00%), Query Frame = 0

Query: 1   MAVVDNSEFSTPLGPPACEKDAKALRFIEETTTNTDSVQQRVLAEILTQNAHTEYVNRFR 60
           MAVVDNSEFSTPLGPPACEKDAKALRFIEETTTNTDSVQQRVLAEILTQNAHTEYVNRFR
Sbjct: 1   MAVVDNSEFSTPLGPPACEKDAKALRFIEETTTNTDSVQQRVLAEILTQNAHTEYVNRFR 60

Query: 61  LNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM 120
           LNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM
Sbjct: 61  LNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM 120

Query: 121 PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK 180
           PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK
Sbjct: 121 PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK 180

Query: 181 SDIFKTRHYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAI 240
           SDIFKTRHYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAI
Sbjct: 181 SDIFKTRHYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAI 240

Query: 241 RFLQLNWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPKPELAEFISSECCTEEWEGI 300
           RFLQLNWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPKPELAEFISSECCTEEWEGI
Sbjct: 241 RFLQLNWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPKPELAEFISSECCTEEWEGI 300

Query: 301 ITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDV 360
           ITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDV
Sbjct: 301 ITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDV 360

Query: 361 TYTIMPNMGYFEFLPHDSSSRAPALSRDSPPRLVDLADVEVGKEYELVITTYAGLVRYRV 420
           TYTIMPNMGYFEFLPHDSSSRAPALSRDSPPRLVDLADVEVGKEYELVITTYAGLVRYRV
Sbjct: 361 TYTIMPNMGYFEFLPHDSSSRAPALSRDSPPRLVDLADVEVGKEYELVITTYAGLVRYRV 420

Query: 421 GDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYT 480
           GDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYT
Sbjct: 421 GDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYT 480

Query: 481 SYADTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSI 540
           SYADTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSI
Sbjct: 481 SYADTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSI 540

Query: 541 GPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSFHFSPSKPH 600
           GPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSFHFSPSKPH
Sbjct: 541 GPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSFHFSPSKPH 600

Query: 601 WTPERRR 607
           WTPERRR
Sbjct: 601 WTPERRR 607

BLAST of CsGy6G028030 vs. ExPASy TrEMBL
Match: A0A5D3CNV8 (Putative indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G004400 PE=3 SV=1)

HSP 1 Score: 1206 bits (3120), Expect = 0.0
Identity = 595/607 (98.02%), Postives = 599/607 (98.68%), Query Frame = 0

Query: 1   MAVVDNSEFSTPLGPPACEKDAKALRFIEETTTNTDSVQQRVLAEILTQNAHTEYVNRFR 60
           MAVVDNSEFS+PLGPPACEKDAKALRFIEETTTN D VQQRVLAEILTQNAHTEYVNRFR
Sbjct: 1   MAVVDNSEFSSPLGPPACEKDAKALRFIEETTTNADLVQQRVLAEILTQNAHTEYVNRFR 60

Query: 61  LNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM 120
           LNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM
Sbjct: 61  LNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM 120

Query: 121 PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK 180
           PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK
Sbjct: 121 PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK 180

Query: 181 SDIFKTRHYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAI 240
           SDIFKTR YDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAI
Sbjct: 181 SDIFKTRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAI 240

Query: 241 RFLQLNWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPKPELAEFISSECCTEEWEGI 300
           RFLQLNWKQLAHDISTGTLNPKITDTCLRECITSKYLTNP PELAEFIS+EC TEEWEGI
Sbjct: 241 RFLQLNWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPNPELAEFISNECYTEEWEGI 300

Query: 301 ITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDV 360
           ITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDV
Sbjct: 301 ITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDV 360

Query: 361 TYTIMPNMGYFEFLPHDSSSRAPALSRDSPPRLVDLADVEVGKEYELVITTYAGLVRYRV 420
           TYTIMPNMGYFEFLPHDSSSRA ALSRDSPPRLVDLADVEVGKEYELVITTYAGL RYRV
Sbjct: 361 TYTIMPNMGYFEFLPHDSSSRASALSRDSPPRLVDLADVEVGKEYELVITTYAGLCRYRV 420

Query: 421 GDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYT 480
           GDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLR+FNTTVVEYT
Sbjct: 421 GDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLRDFNTTVVEYT 480

Query: 481 SYADTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSI 540
           SYADTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSI
Sbjct: 481 SYADTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSI 540

Query: 541 GPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSFHFSPSKPH 600
           GPLEIRVV+NGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTS HFSPSKPH
Sbjct: 541 GPLEIRVVRNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPH 600

Query: 601 WTPERRR 607
           WTPERRR
Sbjct: 601 WTPERRR 607

BLAST of CsGy6G028030 vs. ExPASy TrEMBL
Match: A0A1S3B0G2 (probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo OX=3656 GN=LOC103484816 PE=3 SV=1)

HSP 1 Score: 1206 bits (3120), Expect = 0.0
Identity = 595/607 (98.02%), Postives = 599/607 (98.68%), Query Frame = 0

Query: 1   MAVVDNSEFSTPLGPPACEKDAKALRFIEETTTNTDSVQQRVLAEILTQNAHTEYVNRFR 60
           MAVVDNSEFS+PLGPPACEKDAKALRFIEETTTN D VQQRVLAEILTQNAHTEYVNRFR
Sbjct: 1   MAVVDNSEFSSPLGPPACEKDAKALRFIEETTTNADLVQQRVLAEILTQNAHTEYVNRFR 60

Query: 61  LNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM 120
           LNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM
Sbjct: 61  LNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM 120

Query: 121 PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK 180
           PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK
Sbjct: 121 PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK 180

Query: 181 SDIFKTRHYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAI 240
           SDIFKTR YDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAI
Sbjct: 181 SDIFKTRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAI 240

Query: 241 RFLQLNWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPKPELAEFISSECCTEEWEGI 300
           RFLQLNWKQLAHDISTGTLNPKITDTCLRECITSKYLTNP PELAEFIS+EC TEEWEGI
Sbjct: 241 RFLQLNWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPNPELAEFISNECYTEEWEGI 300

Query: 301 ITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDV 360
           ITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDV
Sbjct: 301 ITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDV 360

Query: 361 TYTIMPNMGYFEFLPHDSSSRAPALSRDSPPRLVDLADVEVGKEYELVITTYAGLVRYRV 420
           TYTIMPNMGYFEFLPHDSSSRA ALSRDSPPRLVDLADVEVGKEYELVITTYAGL RYRV
Sbjct: 361 TYTIMPNMGYFEFLPHDSSSRASALSRDSPPRLVDLADVEVGKEYELVITTYAGLCRYRV 420

Query: 421 GDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYT 480
           GDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLR+FNTTVVEYT
Sbjct: 421 GDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLRDFNTTVVEYT 480

Query: 481 SYADTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSI 540
           SYADTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSI
Sbjct: 481 SYADTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSI 540

Query: 541 GPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSFHFSPSKPH 600
           GPLEIRVV+NGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTS HFSPSKPH
Sbjct: 541 GPLEIRVVRNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSVHFSPSKPH 600

Query: 601 WTPERRR 607
           WTPERRR
Sbjct: 601 WTPERRR 607

BLAST of CsGy6G028030 vs. ExPASy TrEMBL
Match: A0A6J1GH54 (probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita moschata OX=3662 GN=LOC111453877 PE=3 SV=1)

HSP 1 Score: 1120 bits (2898), Expect = 0.0
Identity = 561/607 (92.42%), Postives = 577/607 (95.06%), Query Frame = 0

Query: 1   MAVVDNSEFSTPLGPPACEKDAKALRFIEETTTNTDSVQQRVLAEILTQNAHTEYVNRFR 60
           MAV  +S+ S+PL PP  +KDAKALRFIEE TTNTD+VQQRVLAEIL++NA+TEYVNRFR
Sbjct: 1   MAV--DSQLSSPLPPPPSDKDAKALRFIEEVTTNTDAVQQRVLAEILSRNANTEYVNRFR 60

Query: 61  LNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM 120
           LNGATDRDT+KSK PVVTYEDLQPDIQRIANGDRSPIFSS PISEFLTSSGTSAGERKLM
Sbjct: 61  LNGATDRDTYKSKIPVVTYEDLQPDIQRIANGDRSPIFSSTPISEFLTSSGTSAGERKLM 120

Query: 121 PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK 180
           PTI+EEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK
Sbjct: 121 PTIQEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK 180

Query: 181 SDIFKTRHYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAI 240
           SDIFKTR YDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMR+QVLRVGAVFASGLLRAI
Sbjct: 181 SDIFKTRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAI 240

Query: 241 RFLQLNWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPKPELAEFISSECCTEEWEGI 300
           RFLQLNWKQLAHDISTGTLNPKITD CLRECI SKYLTN  PE+AEFIS EC TEEWEGI
Sbjct: 241 RFLQLNWKQLAHDISTGTLNPKITDPCLRECIVSKYLTNTNPEVAEFISKECSTEEWEGI 300

Query: 301 ITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDV 360
           ITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNL PMCKPS+V
Sbjct: 301 ITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEV 360

Query: 361 TYTIMPNMGYFEFLPHDSSSRAPALSRDSPPRLVDLADVEVGKEYELVITTYAGLVRYRV 420
           TYTIMPNMGYFEFLPHD+SS  P LSRDSPPRLVDLADVEVGKEYELVITTYAGL RYRV
Sbjct: 361 TYTIMPNMGYFEFLPHDTSS-PPVLSRDSPPRLVDLADVEVGKEYELVITTYAGLRRYRV 420

Query: 421 GDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYT 480
           GDVLEVTGFHNAAPQFRFVRRKNVLLSID+DKTDEAELQKAVENASSLLRE NT+VVEYT
Sbjct: 421 GDVLEVTGFHNAAPQFRFVRRKNVLLSIDADKTDEAELQKAVENASSLLRELNTSVVEYT 480

Query: 481 SYADTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSI 540
           SYADTKTIPGHYVIYWELLVKD E  NS P  EILNQCCLAIEESLNSVYRQGRVADNSI
Sbjct: 481 SYADTKTIPGHYVIYWELLVKD-EAANSPPPAEILNQCCLAIEESLNSVYRQGRVADNSI 540

Query: 541 GPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSFHFSPSKPH 600
           GPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRV S HFSPSKPH
Sbjct: 541 GPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVASVHFSPSKPH 600

Query: 601 WTPERRR 607
           WTPERRR
Sbjct: 601 WTPERRR 603

BLAST of CsGy6G028030 vs. ExPASy TrEMBL
Match: A0A6J1IRM5 (probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita maxima OX=3661 GN=LOC111478730 PE=3 SV=1)

HSP 1 Score: 1116 bits (2887), Expect = 0.0
Identity = 558/607 (91.93%), Postives = 575/607 (94.73%), Query Frame = 0

Query: 1   MAVVDNSEFSTPLGPPACEKDAKALRFIEETTTNTDSVQQRVLAEILTQNAHTEYVNRFR 60
           MAV   S+ S+PL PP  ++DAKALRFIEE TTN D+VQQRVLAEIL++NA+TEYVNRFR
Sbjct: 1   MAV--GSQLSSPLPPPPSDRDAKALRFIEEVTTNADAVQQRVLAEILSRNANTEYVNRFR 60

Query: 61  LNGATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLM 120
           LNGATDRDT+KSK PVVTYEDLQPDIQRIANGDRSPIFSS PISEFLTSSGTSAGERKLM
Sbjct: 61  LNGATDRDTYKSKIPVVTYEDLQPDIQRIANGDRSPIFSSTPISEFLTSSGTSAGERKLM 120

Query: 121 PTIKEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK 180
           PTI+EEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK
Sbjct: 121 PTIQEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYK 180

Query: 181 SDIFKTRHYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAI 240
           SDIFKTR YDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMR+QVLRVGAVFASGLLRAI
Sbjct: 181 SDIFKTRPYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMREQVLRVGAVFASGLLRAI 240

Query: 241 RFLQLNWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPKPELAEFISSECCTEEWEGI 300
           RFLQLNWKQLAHDISTGTLNPKITD CLRECI SKYLTNP PE+AEFIS EC TEEWEGI
Sbjct: 241 RFLQLNWKQLAHDISTGTLNPKITDPCLRECIVSKYLTNPNPEVAEFISKECSTEEWEGI 300

Query: 301 ITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDV 360
           ITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNL PMCKPS+V
Sbjct: 301 ITRIWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLKPMCKPSEV 360

Query: 361 TYTIMPNMGYFEFLPHDSSSRAPALSRDSPPRLVDLADVEVGKEYELVITTYAGLVRYRV 420
           TYTIMPNMGYFEFLPH +SS  P LSRDSPPRLVDLADVEVGKEYELVITTYAGL RYRV
Sbjct: 361 TYTIMPNMGYFEFLPHHTSS-PPVLSRDSPPRLVDLADVEVGKEYELVITTYAGLRRYRV 420

Query: 421 GDVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYT 480
           GDVLEVTGFHNAAPQFRFVRRKNVLLSID+DKTDEAELQKAVENASSLLRE NT+VVEYT
Sbjct: 421 GDVLEVTGFHNAAPQFRFVRRKNVLLSIDADKTDEAELQKAVENASSLLRELNTSVVEYT 480

Query: 481 SYADTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSI 540
           SYADTKTIPGHYVIYWELLVKD E  NS P  EILNQCCLAIEESLNSVYRQGRVADNSI
Sbjct: 481 SYADTKTIPGHYVIYWELLVKD-EAANSPPPAEILNQCCLAIEESLNSVYRQGRVADNSI 540

Query: 541 GPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSFHFSPSKPH 600
           GPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRV S HFSP+KPH
Sbjct: 541 GPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVVSVHFSPTKPH 600

Query: 601 WTPERRR 607
           WTPERRR
Sbjct: 601 WTPERRR 603

BLAST of CsGy6G028030 vs. TAIR 10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 938.7 bits (2425), Expect = 2.3e-273
Identity = 462/603 (76.62%), Postives = 516/603 (85.57%), Query Frame = 0

Query: 6   NSEFSTPLGPPACEKDAKALRFIEETTTNTDSVQQRVLAEILTQNAHTEYVNRFRLNGAT 65
           +S  S+PLGPPACEKDAKALRFIEE T N D+VQ+ +LAEIL +NA TEY+ RF L GAT
Sbjct: 4   DSNLSSPLGPPACEKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGAT 63

Query: 66  DRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKE 125
           DRDTFK+K PV+TYEDLQP+IQRIA+GDRSPI S+HPISEFLTSSGTSAGERKLMPTIKE
Sbjct: 64  DRDTFKTKIPVITYEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKE 123

Query: 126 EMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFK 185
           E++RRQLLYSLLMP+MNLYVPGLDKGKG+YFLFVK+ETKTPGGL ARPVLTSYYKS+ F+
Sbjct: 124 ELDRRQLLYSLLMPVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFR 183

Query: 186 TRHYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIRFLQL 245
           +R YDP+N YTSPNEA+LC DSFQSMYTQMLCGLL R  VLRVGAVFASGLLRAIRFLQL
Sbjct: 184 SRPYDPYNVYTSPNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQL 243

Query: 246 NWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPKPELAEFISSECCTEEWEGIITRIW 305
           +W + AHDI  G L+ +ITD  +R+C++   +  P P LAEFI  EC ++ WE IITRIW
Sbjct: 244 HWSRFAHDIELGCLDSEITDPSIRQCMSG--ILKPDPVLAEFIRRECKSDNWEKIITRIW 303

Query: 306 PNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIM 365
           PNTKYLDVIVTGAMAQYIPTLE+YSGGLPMACTMYASSECYFG+NLNPM KPS+V+YTIM
Sbjct: 304 PNTKYLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIM 363

Query: 366 PNMGYFEFLPHDSSSRAPALSRDSPPRLVDLADVEVGKEYELVITTYAGLVRYRVGDVLE 425
           PNM YFEF+P   +            + V+L DV +GKEYELV+TTYAGL RYRVGD+L 
Sbjct: 364 PNMAYFEFIPLGGT------------KAVELVDVNIGKEYELVVTTYAGLCRYRVGDILR 423

Query: 426 VTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLL-REFNTTVVEYTSYAD 485
           VTGFHN+APQF FVRRKNVLLSIDSDKTDE+ELQKAVENASS+L  E  + V EYTSYAD
Sbjct: 424 VTGFHNSAPQFHFVRRKNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYAD 483

Query: 486 TKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSIGPLE 545
           T TIPGHYV+YWELLV+D     SH   E L +CCL +EESLNSVYRQ RVADNS+GPLE
Sbjct: 484 TSTIPGHYVLYWELLVRDGARQPSH---ETLTRCCLGMEESLNSVYRQSRVADNSVGPLE 543

Query: 546 IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSFHFSPSKPHWTPE 605
           IRVV+NGTFEELMDYAISRGASINQYK PRCVNFTPIVELLDSRV S HFSPS PHWTPE
Sbjct: 544 IRVVRNGTFEELMDYAISRGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHWTPE 589

Query: 606 RRR 608
           RRR
Sbjct: 604 RRR 589

BLAST of CsGy6G028030 vs. TAIR 10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 914.1 bits (2361), Expect = 6.1e-266
Identity = 451/604 (74.67%), Postives = 513/604 (84.93%), Query Frame = 0

Query: 4   VDNSEFSTPLGPPACEKDAKALRFIEETTTNTDSVQQRVLAEILTQNAHTEYVNRFRLNG 63
           VD+   S  +     EKD KAL+FIEE T N DSVQ++VL EILT+N++TEY+ RF L+G
Sbjct: 3   VDSPLQSRMVSATTSEKDVKALKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRFDLDG 62

Query: 64  ATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTI 123
             DR TFKSK PVVTYEDL+P+IQRI+NGD SPI SSHPI+EFLTSSGTSAGERKLMPTI
Sbjct: 63  VVDRKTFKSKVPVVTYEDLKPEIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMPTI 122

Query: 124 KEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDI 183
           +E+++RRQLLYSLLMP+MNLYVPGLDKGKGLYFLFVK+E+KT GGL ARPVLTSYYKSD 
Sbjct: 123 EEDLDRRQLLYSLLMPVMNLYVPGLDKGKGLYFLFVKSESKTSGGLPARPVLTSYYKSDH 182

Query: 184 FKTRHYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIRFL 243
           FK R YDP+N YTSPNEA+LC+DS QSMY QMLCGLLMR +VLR+GAVFASGLLRAI FL
Sbjct: 183 FKRRPYDPYNVYTSPNEAILCSDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAISFL 242

Query: 244 QLNWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPKPELAEFISSECCTEEWEGIITR 303
           Q NWK+LA DISTGTL+ +I D  ++  + SK LT P  ELAEF+   C  E WEGIIT+
Sbjct: 243 QNNWKELARDISTGTLSSRIFDPAIKNRM-SKILTKPDQELAEFLVGVCSQENWEGIITK 302

Query: 304 IWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYT 363
           IWPNTKYLDVIVTGAMAQYIPTLE+YSGGLPMACTMYASSE YFG+NL PMCKPS+V+YT
Sbjct: 303 IWPNTKYLDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYT 362

Query: 364 IMPNMGYFEFLPHDSSSRAPALSRDSPPRLVDLADVEVGKEYELVITTYAGLVRYRVGDV 423
           IMPNM YFEFLPH+      A +      LV+LA+VEVGKEYELVITTYAGL RYRVGD+
Sbjct: 363 IMPNMAYFEFLPHNHDGDGAAEASLDETSLVELANVEVGKEYELVITTYAGLYRYRVGDI 422

Query: 424 LEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYTSYA 483
           L VTGFHN+APQF+F+RRKNVLLS++SDKTDEAELQKAVENAS L  E  T V+EYTSYA
Sbjct: 423 LRVTGFHNSAPQFKFIRRKNVLLSVESDKTDEAELQKAVENASRLFAEQGTRVIEYTSYA 482

Query: 484 DTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSIGPL 543
           +TKTIPGHYVIYWELL +D    N+  ++E++ +CCL +EESLNSVYRQ RVAD SIGPL
Sbjct: 483 ETKTIPGHYVIYWELLGRDQS--NALMSEEVMAKCCLEMEESLNSVYRQSRVADKSIGPL 542

Query: 544 EIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSFHFSPSKPHWTP 603
           EIRVV+NGTFEELMDYAISRGASINQYK PRCV+FTPI+ELLDSRV S HFSPS PHW+P
Sbjct: 543 EIRVVRNGTFEELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSAHFSPSLPHWSP 602

Query: 604 ERRR 608
           ERRR
Sbjct: 603 ERRR 603

BLAST of CsGy6G028030 vs. TAIR 10
Match: AT2G23170.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 896.3 bits (2315), Expect = 1.3e-260
Identity = 449/603 (74.46%), Postives = 508/603 (84.25%), Query Frame = 0

Query: 6   NSEFSTPLGPPACEKDAKALRFIEETTTNTDSVQQRVLAEILTQNAHTEYVNRFRLNGAT 65
           +S   +P+      KD KALRFIEE T N D VQ++V+ EIL++N+ TEY+ RF L G T
Sbjct: 4   DSALRSPMMHSPSTKDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLKGFT 63

Query: 66  DRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKE 125
           DR TFK+K PVV Y+DL+P+IQRIANGDRS I SS+PI+EFLTSSGTSAGERKLMPTI E
Sbjct: 64  DRKTFKTKVPVVIYDDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPTIDE 123

Query: 126 EMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFK 185
           +M+RRQLLYSLLMP+MNLYVPGLDKGK LYFLFVK E+KTPGGL ARPVLTSYYKS+ FK
Sbjct: 124 DMDRRQLLYSLLMPVMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSEQFK 183

Query: 186 TRHYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIRFLQL 245
            R  DP+N YTSPNEA+LC DS QSMYTQMLCGLLMR +VLR+GAVFASGLLRAI FLQ 
Sbjct: 184 RRPNDPYNVYTSPNEAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGFLQT 243

Query: 246 NWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPKPELAEFISSECCTE-EWEGIITRI 305
           NWK+LA DISTGTL+ +I+D  ++E + SK LT P  ELA+FI+S C  +  WEGIIT+I
Sbjct: 244 NWKELADDISTGTLSSRISDPAIKESM-SKILTKPDQELADFITSVCGQDNSWEGIITKI 303

Query: 306 WPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTI 365
           WPNTKYLDVIVTGAMAQYIP LE+YSGGLPMACTMYASSE YFG+NL PMCKPS+V+YTI
Sbjct: 304 WPNTKYLDVIVTGAMAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTI 363

Query: 366 MPNMGYFEFLPHDSSSRAPALSRDSPPRLVDLADVEVGKEYELVITTYAGLVRYRVGDVL 425
           MPNM YFEFLPH       +        LV+LADVEVGKEYELVITTYAGL RYRVGD+L
Sbjct: 364 MPNMAYFEFLPHHEVPTEKS-------ELVELADVEVGKEYELVITTYAGLNRYRVGDIL 423

Query: 426 EVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYTSYAD 485
           +VTGF+N+APQF+FVRRKNVLLSI+SDKTDEAELQ AVENAS LL E  T V+EYTSYA+
Sbjct: 424 QVTGFYNSAPQFKFVRRKNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAE 483

Query: 486 TKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSIGPLE 545
           TKTIPGHYVIYWELLVKD     + P DE++ +CCL +EESLNSVYRQ RVAD SIGPLE
Sbjct: 484 TKTIPGHYVIYWELLVKD---QTNPPNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLE 543

Query: 546 IRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSFHFSPSKPHWTPE 605
           IRVVKNGTFEELMDYAISRGASINQYK PRCV+FTPI+ELLDSRV S HFSP+ PHW+PE
Sbjct: 544 IRVVKNGTFEELMDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSTHFSPALPHWSPE 595

Query: 606 RRR 608
           RRR
Sbjct: 604 RRR 595

BLAST of CsGy6G028030 vs. TAIR 10
Match: AT1G59500.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 858.2 bits (2216), Expect = 4.0e-249
Identity = 431/605 (71.24%), Postives = 497/605 (82.15%), Query Frame = 0

Query: 4   VDNSEFSTPLGPPACEKDAKALRFIEETTTNTDSVQQRVLAEILTQNAHTEYVNRFRLNG 63
           VD+   S    P   E + KAL+FIEE T N DSVQ++VL EIL++N++TEY+ RF LNG
Sbjct: 3   VDSLLQSGMASPTTSETEVKALKFIEEITRNPDSVQEKVLGEILSRNSNTEYLKRFDLNG 62

Query: 64  ATDRDTFKSKFPVVTYEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTI 123
           A DR +FKSK PVV YEDL+ DIQRI+NGDRSPI SSHPI+EFLTSSGTSAGERKLMPTI
Sbjct: 63  AVDRKSFKSKVPVVIYEDLKTDIQRISNGDRSPILSSHPITEFLTSSGTSAGERKLMPTI 122

Query: 124 KEEMERRQLLYSLLMPIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDI 183
           +E++ RRQLL +LLMP+MNLYVPGLDKGKGLYFLFVK+E+ T GGL ARP LTSYYKSD 
Sbjct: 123 EEDINRRQLLGNLLMPVMNLYVPGLDKGKGLYFLFVKSESTTSGGLPARPALTSYYKSDY 182

Query: 184 FKTRHYDPFNDYTSPNEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIRFL 243
           F+T   D  + YTSP EA+LC DS QSMYTQMLCGLLMR +V R+GAVF SGLLRAI FL
Sbjct: 183 FRTSDSD--SVYTSPKEAILCCDSSQSMYTQMLCGLLMRHEVNRLGAVFPSGLLRAISFL 242

Query: 244 QLNWKQLAHDISTGTLNPKITDTCLRECITSKYLTNPKPELAEFISSECCTEEWEGIITR 303
           Q NWK+L+ DISTGTL+ KI D  ++  + S  L  P  ELAEF+   C  E WEGIIT+
Sbjct: 243 QNNWKELSQDISTGTLSSKIFDHAIKTRM-SNILNKPDQELAEFLIGVCSQENWEGIITK 302

Query: 304 IWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYT 363
           IWPNTKYLDVIVTGAMA+YIP LE+YSGGLPMA  +YASSE YFG+NLNPMCKPS+V+YT
Sbjct: 303 IWPNTKYLDVIVTGAMAEYIPMLEYYSGGLPMASMIYASSESYFGINLNPMCKPSEVSYT 362

Query: 364 IMPNMGYFEFLP--HDSSSRAPALSRDSPPRLVDLADVEVGKEYELVITTYAGLVRYRVG 423
           I PNM YFEFLP  HD      A S      LV+LADVEVGKEYELVITTYAGL RYRVG
Sbjct: 363 IFPNMAYFEFLPHNHDGDGGVEATS------LVELADVEVGKEYELVITTYAGLYRYRVG 422

Query: 424 DVLEVTGFHNAAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYTS 483
           D+L VTGFHN+APQF+F+RR+NVLLSI+SDKTDEA+LQKAVENAS LL E  T V+EYTS
Sbjct: 423 DILRVTGFHNSAPQFKFIRRENVLLSIESDKTDEADLQKAVENASRLLAEQGTRVIEYTS 482

Query: 484 YADTKTIPGHYVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSIG 543
           YADTKTIPGHYVIYWELL +D    N+ P+DE++ +CCL +EESLN+VYRQ RV+D SIG
Sbjct: 483 YADTKTIPGHYVIYWELLSRDQS--NALPSDEVMAKCCLEMEESLNAVYRQSRVSDKSIG 542

Query: 544 PLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSFHFSPSKPHW 603
           PLEIRVV+NGTFEELMD++ISRG+SINQYK PRCV+ TPI++LLDSRV S HFSPS PHW
Sbjct: 543 PLEIRVVQNGTFEELMDFSISRGSSINQYKVPRCVSLTPIMKLLDSRVVSAHFSPSLPHW 596

Query: 604 TPERR 607
           +PERR
Sbjct: 603 SPERR 596

BLAST of CsGy6G028030 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 796.6 bits (2056), Expect = 1.4e-230
Identity = 383/596 (64.26%), Postives = 471/596 (79.03%), Query Frame = 0

Query: 19  EKDAKALRFIEETTTNTDSVQQRVLAEILTQNAHTEYVNRFRLNGATDRDTFKSKFPVVT 78
           EK+   L+FIE+ TTN D VQ+RVL EIL++NA  EY+ R  L G TDR+TFK   PVVT
Sbjct: 19  EKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPVVT 78

Query: 79  YEDLQPDIQRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIKEEMERRQLLYSLLM 138
           YED+QP+I RIANGD+S +  S+PISEFLTSSGTS GERKLMPTI+EE++RR LLYSLLM
Sbjct: 79  YEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLM 138

Query: 139 PIMNLYVPGLDKGKGLYFLFVKAETKTPGGLVARPVLTSYYKSDIFKTRHYDPFNDYTSP 198
           P+M+ +VPGLDKGKG+YFLF+K+E+KTPGGL ARPVLTSYYKS  FK R YDP+ +YTSP
Sbjct: 139 PVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYTSP 198

Query: 199 NEAVLCADSFQSMYTQMLCGLLMRDQVLRVGAVFASGLLRAIRFLQLNWKQLAHDISTGT 258
           N+ +LC+DS+QSMY+QMLCGL    +VLRVGAVFASG +RAI+FL+ +W +LA DI TGT
Sbjct: 199 NQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRTGT 258

Query: 259 LNPKITDTCLRECITSKYLTNPKPELAEFISSECCTEEWEGIITRIWPNTKYLDVIVTGA 318
           L+ +ITD+ +RE +    +  P P+LA+F+ SEC    W+GIITR+WPNTKY+DVIVTG 
Sbjct: 259 LSSEITDSSVREAVGE--ILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGT 318

Query: 319 MAQYIPTLEFYSGGLPMACTMYASSECYFGVNLNPMCKPSDVTYTIMPNMGYFEFLP--- 378
           M+QYIPTL++YS GLP+ CTMYASSECYFGVNL P+CKPS+V+YT++PNM YFEFLP   
Sbjct: 319 MSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHR 378

Query: 379 ----HDSSSRAPALSRDSPPRLVDLADVEVGKEYELVITTYAGLVRYRVGDVLEVTGFHN 438
                 S S   AL+      LVDL DV++G+EYELV+TTYAGL RYRVGDVL V GF N
Sbjct: 379 NSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFKN 438

Query: 439 AAPQFRFVRRKNVLLSIDSDKTDEAELQKAVENASSLLREFNTTVVEYTSYADTKTIPGH 498
            APQF F+ RKNV+LSIDSDKTDE ELQ AV+NA + L  F+ ++ EYTSYADT +IPGH
Sbjct: 439 NAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGH 498

Query: 499 YVIYWELLVKDNETMNSHPTDEILNQCCLAIEESLNSVYRQGRVADNSIGPLEIRVVKNG 558
           YV++WEL +  N  +       +   CCL IEESLNSVYRQGRV+D SIGPLEI++V++G
Sbjct: 499 YVLFWELCLNGNTPI----PPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESG 558

Query: 559 TFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVTSFHFSPSKPHWTPERRR 608
           TF++LMDYAIS GASINQYK PRCV F PI+ELL+SRV   +FSP  P W+P  ++
Sbjct: 559 TFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPKWSPGHKQ 608

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O823333.2e-27276.62Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=... [more]
O221901.8e-25974.46Indole-3-acetic acid-amido synthetase GH3.3 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
A3BLS08.0e-25573.04Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=399... [more]
Q0D4Z68.0e-25573.04Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. japonica OX=3... [more]
P0C0M27.0e-25171.21Probable indole-3-acetic acid-amido synthetase GH3.2 OS=Oryza sativa subsp. japo... [more]
Match NameE-valueIdentityDescription
XP_004141893.10.0100.00probable indole-3-acetic acid-amido synthetase GH3.1 [Cucumis sativus] >KGN48572... [more]
XP_008440335.10.098.02PREDICTED: probable indole-3-acetic acid-amido synthetase GH3.1 [Cucumis melo] >... [more]
XP_038881175.10.095.22probable indole-3-acetic acid-amido synthetase GH3.1 [Benincasa hispida][more]
KAG6604151.10.092.59putative indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma ... [more]
XP_023543141.10.092.59probable indole-3-acetic acid-amido synthetase GH3.1 [Cucurbita pepo subsp. pepo... [more]
Match NameE-valueIdentityDescription
A0A0A0KJM50.0100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G493310 PE=3 SV=1[more]
A0A5D3CNV80.098.02Putative indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo var. makuwa... [more]
A0A1S3B0G20.098.02probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucumis melo OX=3656 GN=... [more]
A0A6J1GH540.092.42probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita moschata OX=36... [more]
A0A6J1IRM50.091.93probable indole-3-acetic acid-amido synthetase GH3.1 OS=Cucurbita maxima OX=3661... [more]
Match NameE-valueIdentityDescription
AT2G14960.12.3e-27376.62Auxin-responsive GH3 family protein [more]
AT4G37390.16.1e-26674.67Auxin-responsive GH3 family protein [more]
AT2G23170.11.3e-26074.46Auxin-responsive GH3 family protein [more]
AT1G59500.14.0e-24971.24Auxin-responsive GH3 family protein [more]
AT5G54510.11.4e-23064.26Auxin-responsive GH3 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004993GH3 familyPFAMPF03321GH3coord: 28..578
e-value: 5.3E-201
score: 669.0
IPR004993GH3 familyPANTHERPTHR31901GH3 DOMAIN-CONTAINING PROTEINcoord: 10..607
NoneNo IPR availablePANTHERPTHR31901:SF7INDOLE-3-ACETIC ACID-AMIDO SYNTHETASE GH3.2-RELATEDcoord: 10..607

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy6G028030.1CsGy6G028030.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
molecular_function GO:0016881 acid-amino acid ligase activity