CsGy6G026350 (gene) Cucumber (Gy14) v2.1

BLAST of CsGy6G026350 vs. ExPASy Swiss-Prot
Match: C5H8J1 (Crossover junction endonuclease EME1B OS=Arabidopsis thaliana OX=3702 GN=EME1B PE=1 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 1.8e-139
Identity = 289/565 (51.15%), Postives = 393/565 (69.56%), Query Frame = 0

Query: 1   MSQPIILSDEEDRYSTPIPIPSKKRRTESDLLPNIKSTVLVLDDDPTPQKSNHNSAAS-- 60
           M+  I++SD ED+ +TP+P  SK+ R          S +L+ D DPTPQK    S+ +  
Sbjct: 1   MNDHILISDGEDQ-TTPLPSLSKRARKYP------ISAILISDSDPTPQKQPPESSFTPI 60

Query: 61  FVPETPLSPQPSSDVAIVKCTKAGLNLHARDLNSDQKFAGISGLICLESDNESESDSQKK 120
           FVPETPL    S D ++VKC+       A   N + KF   SG   +  D+E E   + +
Sbjct: 61  FVPETPL----SDDFSVVKCS---FGSRALASNREDKF---SGKRIISLDSEFEDSPRPE 120

Query: 121 KCEQSEWIGSDCYEMKNIGASLPFDSAGSGSSLGNDDLQEISGDMKHSTF--GRDDDIYQ 180
             +++E + +   E +    +   ++    + +   +L + +  M   +F     +D  +
Sbjct: 121 TSKKNESVLAGLREPRFGLEAETSEAYYKNTRIPETNLDDDTSWMHEVSFRSSPTNDTIE 180

Query: 181 VHDYPEEDNVGMEQ--NDNSMKTTGRKANAEKVTRKKATKDDKISLMEEKKKKKELEKLQ 240
           V    E++++ +E+      ++TT      E + +K+ +K+DK S MEEKK +KE E+L+
Sbjct: 181 VVSDQEKEDISVEKIGRKKKIRTTTLPVPGEALPKKRQSKEDKTSAMEEKKLRKEQERLE 240

Query: 241 KAAQKAEAAEMKKMQKEKQKWEKGKLALKSIVAEIDAKVVELGSIGGHLLTRFAEKGITF 300
           KAA KAE AE K+++KEK+KWEKGKLALKSIVAEID KV+E GSIGG LL+RF+EKGIT 
Sbjct: 241 KAASKAEEAERKRLEKEKKKWEKGKLALKSIVAEIDTKVLE-GSIGGLLLSRFSEKGITI 300

Query: 301 RIKSNPIERSILWTMNVPEHIS-ASSKGLEISYVLLVYEAENFCELLSKESFMDHVARVQ 360
            +  NPIERSI+WTM +PE I+    +G +I Y+LLVY+AE FC L++   F++ ++RVQ
Sbjct: 301 HVGPNPIERSIVWTMTIPEDIAPLFPQGPKIPYLLLVYDAEEFCNLVANGKFLEIISRVQ 360

Query: 361 SRYPSHTICYLTNRLMAFINKREQEKYKNLTCSNDWIRPPVEEELAKLTTHFVKVRSRQC 420
            RYPS+T+C LTN+LM+++ KRE+E+YKN     +W RPP++E LAKLTTH+VKV SR C
Sbjct: 361 DRYPSYTVCCLTNKLMSYVKKREKEEYKN---PGNWRRPPIDEVLAKLTTHYVKVHSRHC 420

Query: 421 VDEAEVADHIVGLTCGLASCQFRKKLTHLSVNANGSIIPKDCVDRNMIKKSLWLKALVAI 480
           VDEAEVA+HIVGLT  LASCQFRKKLT LSV+ANG+++ KD VD+++IKKS WLKALVAI
Sbjct: 421 VDEAEVAEHIVGLTSSLASCQFRKKLTMLSVSANGALVSKDSVDKHLIKKSPWLKALVAI 480

Query: 481 PKVQPRFAIAVWKKYPTMKSLLSVYMDPRKSVHEKEFLLKDLTIEGLLGDDRRLGEICSK 540
           PKVQPR+A+AVWKKYP+MKSLL VYMD  KSVHEKEFLLKDL +EGL+G D RLGEICSK
Sbjct: 481 PKVQPRYALAVWKKYPSMKSLLKVYMDRNKSVHEKEFLLKDLKVEGLVGGDIRLGEICSK 540

Query: 541 RVYRILMAQNGNMRTDDVEEGADLF 559
           R+YR+LM+ +G ++TDDVE GA  F
Sbjct: 541 RIYRVLMSHDGAIKTDDVENGAAFF 544

BLAST of CsGy6G026350 vs. ExPASy Swiss-Prot
Match: Q84M98 (Crossover junction endonuclease EME1A OS=Arabidopsis thaliana OX=3702 GN=EME1A PE=1 SV=1)

HSP 1 Score: 424.1 bits (1089), Expect = 2.5e-117
Identity = 262/565 (46.37%), Postives = 370/565 (65.49%), Query Frame = 0

Query: 1   MSQPIILSDEEDRYSTPIPIPSKKRRTESDLLPNIKSTVLVLDDDPTPQKSNHNSAAS-- 60
           MS  I++SD ED  + P    ++K RT +D         L LD +P+ QK    SA++  
Sbjct: 1   MSDFILISDGEDEATPPPSKRARKNRTPTD---------LNLDTEPSLQKQPPGSASTPF 60

Query: 61  FVPETPLSPQPSSDVAIVKCTKAGLNLHARDLNSDQKFAGISGLICLESDNESESDSQKK 120
           F+ ETPL    S DV ++K +  G    A     +  F G   +I LESD+E     +  
Sbjct: 61  FLDETPL----SDDVTVLK-SSFGSGTGASS-GRENNFFG-KRVISLESDSEDSPGPESS 120

Query: 121 KCEQSEWIGSDCYEMKNIGASLPFDSAGSGSSLGNDDLQEISGDMKHSTFGRDDDIYQVH 180
           K  + E + +D ++       L F   GS  +  +DD   +      S+  +  D  +V 
Sbjct: 121 K--KYEPVYTDSWKKP---CRLEF---GSSDANSDDDPSWMRRASFQSSLSK--DAIEVD 180

Query: 181 DYPEEDNVGMEQNDNSMKTTGRKA---NAEKVTRKKATKDDKISLMEEKKKKKELEKLQK 240
              E+++ G+E+     +T   K+   +A+ + +KK +KD+K    EEKK +KE EKLQK
Sbjct: 181 SDHEKEDTGVEKMGRKKQTITSKSTSLSADSLPKKKMSKDEKTRAAEEKKLQKEQEKLQK 240

Query: 241 AAQKAEAAEMKKMQKEKQKWEKGK-LALKSIVAEIDAKVVELGSIGGHLLTRFAEKGITF 300
           AA KAE AE KK+++EKQKW K K  ALK IVA ID KV+E GS GG L++   EK IT+
Sbjct: 241 AASKAEDAEHKKLEREKQKWAKEKDKALKCIVAWIDNKVLE-GSFGGLLISGLKEKCITY 300

Query: 301 RIKSNPIERSILWTMNVPEHISAS-SKGLEISYVLLVYEAENFCELLSKESFMDHVARVQ 360
            + +NPI+RSI+WTM +PE I+ S   G +I YVLL+YEAE+FC L++K+  +++V RV+
Sbjct: 301 HVTTNPIQRSIVWTMTLPEDIAQSLPLGSKIPYVLLLYEAEDFCNLVAKKELLENVYRVR 360

Query: 361 SRYPSHTICYLTNRLMAFINKREQEKYKNLTCSNDWIRPPVEEELAKLTTHFVKVRSRQC 420
             YPS+T+CYLTN+L++++NK+E+ +YK+      W +PP++E +AKL+TH++ V SR C
Sbjct: 361 DEYPSYTMCYLTNKLLSYVNKKERVEYKDPVNGCGWRKPPIDEAIAKLSTHYIGVHSRHC 420

Query: 421 VDEAEVADHIVGLTCGLASCQFRKKLTHLSVNANGSIIPKDCVDRNMIKKSLWLKALVAI 480
           VDEAEVADH+V LT  LA CQ RKKLT LSV A+G+++ K+  D+++I++S+WLK LVAI
Sbjct: 421 VDEAEVADHVVRLTSSLAHCQVRKKLTRLSVYADGTLMSKNAADKHLIRESIWLKVLVAI 480

Query: 481 PKVQPRFAIAVWKKYPTMKSLLSVYMDPRKSVHEKEFLLKDLTIEGLLGDDRRLGEICSK 540
           PKVQPR+AIAV KKYP++KSLL VYMDP  SVHEKEFLLKDL +E L+G D  +GE CSK
Sbjct: 481 PKVQPRYAIAVSKKYPSLKSLLKVYMDPNISVHEKEFLLKDLKVENLVGRDTSVGEACSK 538

Query: 541 RVYRILMAQNGNMRTDDVEEGADLF 559
           R+YR+LM+ +G ++TDDVE GA  F
Sbjct: 541 RIYRVLMSLDGTIKTDDVENGAASF 538

BLAST of CsGy6G026350 vs. ExPASy Swiss-Prot
Match: Q0J9J6 (Crossover junction endonuclease EME1 OS=Oryza sativa subsp. japonica OX=39947 GN=EME1 PE=2 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 2.7e-103
Identity = 257/628 (40.92%), Postives = 338/628 (53.82%), Query Frame = 0

Query: 9   DEEDRYSTPIPIPSKKRRTESDLLPNIKSTVLVLDDDPTPQKSN-----HNSAASFVPET 68
           D  D +S P P P  KRR        + ++ +VLDD PTP K        + +AS V +T
Sbjct: 43  DSPDAFS-PSP-PDPKRR-------QLAASTIVLDDTPTPPKRRPPPVAADRSASVVADT 102

Query: 69  PLSPQPSS--------DVAIVKCTKAGLNLHARD-----------------------LNS 128
           P S  P S        D           +L A D                        NS
Sbjct: 103 PRSFVPCSLRNRAIAGDTPDSVLPSPSFHLDAPDSATPGSDVPCSVGLDDIVPETPGFNS 162

Query: 129 D-----------------QKFAGISGLICLESDNESE----------------------- 188
                             +KF+G+S  I L+SD+E +                       
Sbjct: 163 PRLARPPAVPGLTSPMTARKFSGVSCPISLDSDDELDDTVYRESLTRTPSNMAKPEHAIQ 222

Query: 189 --SDSQKKKCEQSEWIGSDCYEMKNIGASLPFDSAGSGSSLGNDDLQEISGDMKHSTFGR 248
             + S   K E ++      Y   N+G      +  +  +   +     +   + ST   
Sbjct: 223 PCTTSCTDKVENTKSTDKKDYSKSNVGYQTNNSACKNNGTTSYNQPPRANSPCEDSTLKE 282

Query: 249 DDDIYQVHDYPEEDNVGMEQNDNSMKTTGRKANAEKVTRKKATKDDKISLMEEKKKKKEL 308
            D     H           Q +N++    RK   ++   K+  K+ K   +EEKK+K+  
Sbjct: 283 ADPFINNH---------CPQEENALPIEERKKKQQE--EKRLKKEKKAREIEEKKQKRLE 342

Query: 309 EKLQKAAQKAEAAEMKKMQKEKQKWEKGKLALKSIVAEIDAKVVELGSIGGHLLTRFAEK 368
            K QK A KAE AE+KK++KEK+KWE GKLA K IVAEID+ V+E GS+GGHL+  F EK
Sbjct: 343 TKKQKEAMKAELAELKKLEKEKKKWESGKLATKCIVAEIDSSVIESGSVGGHLVQGFHEK 402

Query: 369 GITFRIKSNPIERSILWTMNVP-EHISASSKGLEISYVLLVYEAENFCELLSKESFMDHV 428
           G+ FR+ SN I+ SILW M +P E     +   ++ Y+L V +AE FC+L+S  + +DHV
Sbjct: 403 GLCFRVTSNSIKGSILWKMQIPNEFTQDQASTSQVPYILFVLQAEEFCDLVSGGTLLDHV 462

Query: 429 ARVQSRYPSHTICYLTNRLMAFINKREQEKYKNLTC-SNDWIRPPVEEELAKLTTHFVKV 488
            +V+ +YP  TICY+TN+LM+FI +REQ +Y   T  SN W RPPVEE L KL TH+ +V
Sbjct: 463 QKVRRQYPEFTICYVTNKLMSFIKRREQNQYNKTTSNSNSWKRPPVEEALCKLATHYARV 522

Query: 489 RSRQCVDEAEVADHIVGLTCGLASCQFRKKLTHLSVNANGSIIPKDCVDRNMIKKSLWLK 548
           RSR C DEAEV +HIVGLT  LA+C+FRK LT LSV+ANGS I K CVD++ IKKS WLK
Sbjct: 523 RSRHCTDEAEVTEHIVGLTYSLANCKFRKPLTWLSVHANGSNISKGCVDKDRIKKSAWLK 582

Query: 549 ALVAIPKVQPRFAIAVWKKYPTMKSLLSVYMDPRKSVHEKEFLLKDLTIEGLLGD-DRRL 556
           +LVAIP V P  AIA+ KKYP+M+SLL+VYMD  KSVHEKE LL+DL +EG LGD  RRL
Sbjct: 583 SLVAIPGVSPGQAIAIEKKYPSMRSLLNVYMDDSKSVHEKEHLLEDLRLEGPLGDFKRRL 642

BLAST of CsGy6G026350 vs. NCBI nr
Match: XP_004142045.1 (crossover junction endonuclease EME1B [Cucumis sativus] >KGN48409.1 hypothetical protein Csa_002767 [Cucumis sativus])

HSP 1 Score: 1096 bits (2834), Expect = 0.0
Identity = 563/563 (100.00%), Postives = 563/563 (100.00%), Query Frame = 0

Query: 1   MSQPIILSDEEDRYSTPIPIPSKKRRTESDLLPNIKSTVLVLDDDPTPQKSNHNSAASFV 60
           MSQPIILSDEEDRYSTPIPIPSKKRRTESDLLPNIKSTVLVLDDDPTPQKSNHNSAASFV
Sbjct: 1   MSQPIILSDEEDRYSTPIPIPSKKRRTESDLLPNIKSTVLVLDDDPTPQKSNHNSAASFV 60

Query: 61  PETPLSPQPSSDVAIVKCTKAGLNLHARDLNSDQKFAGISGLICLESDNESESDSQKKKC 120
           PETPLSPQPSSDVAIVKCTKAGLNLHARDLNSDQKFAGISGLICLESDNESESDSQKKKC
Sbjct: 61  PETPLSPQPSSDVAIVKCTKAGLNLHARDLNSDQKFAGISGLICLESDNESESDSQKKKC 120

Query: 121 EQSEWIGSDCYEMKNIGASLPFDSAGSGSSLGNDDLQEISGDMKHSTFGRDDDIYQVHDY 180
           EQSEWIGSDCYEMKNIGASLPFDSAGSGSSLGNDDLQEISGDMKHSTFGRDDDIYQVHDY
Sbjct: 121 EQSEWIGSDCYEMKNIGASLPFDSAGSGSSLGNDDLQEISGDMKHSTFGRDDDIYQVHDY 180

Query: 181 PEEDNVGMEQNDNSMKTTGRKANAEKVTRKKATKDDKISLMEEKKKKKELEKLQKAAQKA 240
           PEEDNVGMEQNDNSMKTTGRKANAEKVTRKKATKDDKISLMEEKKKKKELEKLQKAAQKA
Sbjct: 181 PEEDNVGMEQNDNSMKTTGRKANAEKVTRKKATKDDKISLMEEKKKKKELEKLQKAAQKA 240

Query: 241 EAAEMKKMQKEKQKWEKGKLALKSIVAEIDAKVVELGSIGGHLLTRFAEKGITFRIKSNP 300
           EAAEMKKMQKEKQKWEKGKLALKSIVAEIDAKVVELGSIGGHLLTRFAEKGITFRIKSNP
Sbjct: 241 EAAEMKKMQKEKQKWEKGKLALKSIVAEIDAKVVELGSIGGHLLTRFAEKGITFRIKSNP 300

Query: 301 IERSILWTMNVPEHISASSKGLEISYVLLVYEAENFCELLSKESFMDHVARVQSRYPSHT 360
           IERSILWTMNVPEHISASSKGLEISYVLLVYEAENFCELLSKESFMDHVARVQSRYPSHT
Sbjct: 301 IERSILWTMNVPEHISASSKGLEISYVLLVYEAENFCELLSKESFMDHVARVQSRYPSHT 360

Query: 361 ICYLTNRLMAFINKREQEKYKNLTCSNDWIRPPVEEELAKLTTHFVKVRSRQCVDEAEVA 420
           ICYLTNRLMAFINKREQEKYKNLTCSNDWIRPPVEEELAKLTTHFVKVRSRQCVDEAEVA
Sbjct: 361 ICYLTNRLMAFINKREQEKYKNLTCSNDWIRPPVEEELAKLTTHFVKVRSRQCVDEAEVA 420

Query: 421 DHIVGLTCGLASCQFRKKLTHLSVNANGSIIPKDCVDRNMIKKSLWLKALVAIPKVQPRF 480
           DHIVGLTCGLASCQFRKKLTHLSVNANGSIIPKDCVDRNMIKKSLWLKALVAIPKVQPRF
Sbjct: 421 DHIVGLTCGLASCQFRKKLTHLSVNANGSIIPKDCVDRNMIKKSLWLKALVAIPKVQPRF 480

Query: 481 AIAVWKKYPTMKSLLSVYMDPRKSVHEKEFLLKDLTIEGLLGDDRRLGEICSKRVYRILM 540
           AIAVWKKYPTMKSLLSVYMDPRKSVHEKEFLLKDLTIEGLLGDDRRLGEICSKRVYRILM
Sbjct: 481 AIAVWKKYPTMKSLLSVYMDPRKSVHEKEFLLKDLTIEGLLGDDRRLGEICSKRVYRILM 540

Query: 541 AQNGNMRTDDVEEGADLFRSQLS 563
           AQNGNMRTDDVEEGADLFRSQLS
Sbjct: 541 AQNGNMRTDDVEEGADLFRSQLS 563

BLAST of CsGy6G026350 vs. NCBI nr
Match: XP_008440090.1 (PREDICTED: crossover junction endonuclease EME1B isoform X1 [Cucumis melo])

HSP 1 Score: 1055 bits (2728), Expect = 0.0
Identity = 542/563 (96.27%), Postives = 552/563 (98.05%), Query Frame = 0

Query: 1   MSQPIILSDEEDRYSTPIPIPSKKRRTESDLLPNIKSTVLVLDDDPTPQKSNHNSAASFV 60
           MSQPI+LSDEEDRYSTPIPIPSKKRRTESDLLP+IKSTVLVLDDDPTPQKSNHNSAASFV
Sbjct: 1   MSQPIVLSDEEDRYSTPIPIPSKKRRTESDLLPSIKSTVLVLDDDPTPQKSNHNSAASFV 60

Query: 61  PETPLSPQPSSDVAIVKCTKAGLNLHARDLNSDQKFAGISGLICLESDNESESDSQKKKC 120
           PETPLSPQ SSDVAIVKCTKAGLNLHARDLNSDQKFAGISGLICLESDNESESDSQK+KC
Sbjct: 61  PETPLSPQLSSDVAIVKCTKAGLNLHARDLNSDQKFAGISGLICLESDNESESDSQKRKC 120

Query: 121 EQSEWIGSDCYEMKNIGASLPFDSAGSGSSLGNDDLQEISGDMKHSTFGRDDDIYQVHDY 180
           EQSEWIGSDCYEMKNIGASLPFDSAGSGSS GNDDLQEISGD KHS FGRD DIYQVHDY
Sbjct: 121 EQSEWIGSDCYEMKNIGASLPFDSAGSGSSHGNDDLQEISGDTKHSAFGRDGDIYQVHDY 180

Query: 181 PEEDNVGMEQNDNSMKTTGRKANAEKVTRKKATKDDKISLMEEKKKKKELEKLQKAAQKA 240
           PE+DNVGMEQNDNS+KTTGRKANAEKV RKKATKDDKI LMEEKKKKKELEKLQKAAQKA
Sbjct: 181 PEKDNVGMEQNDNSIKTTGRKANAEKVMRKKATKDDKIRLMEEKKKKKELEKLQKAAQKA 240

Query: 241 EAAEMKKMQKEKQKWEKGKLALKSIVAEIDAKVVELGSIGGHLLTRFAEKGITFRIKSNP 300
           EAAEMKKMQKEKQKWEKGKLALKSIVAEIDAKVVELGSIGGHLLTRFAEKGITFRIKSNP
Sbjct: 241 EAAEMKKMQKEKQKWEKGKLALKSIVAEIDAKVVELGSIGGHLLTRFAEKGITFRIKSNP 300

Query: 301 IERSILWTMNVPEHISASSKGLEISYVLLVYEAENFCELLSKESFMDHVARVQSRYPSHT 360
           IERSILWTMNVPEHISASSKGLEISYVLLVYEAENFCELLSKESFMDHVARVQSRYPSHT
Sbjct: 301 IERSILWTMNVPEHISASSKGLEISYVLLVYEAENFCELLSKESFMDHVARVQSRYPSHT 360

Query: 361 ICYLTNRLMAFINKREQEKYKNLTCSNDWIRPPVEEELAKLTTHFVKVRSRQCVDEAEVA 420
           ICYLTNRLMAFINKREQEKYKN TC  DWIRPPVEEELAKL+THFVKVRSRQCVDEAEVA
Sbjct: 361 ICYLTNRLMAFINKREQEKYKNSTCCTDWIRPPVEEELAKLSTHFVKVRSRQCVDEAEVA 420

Query: 421 DHIVGLTCGLASCQFRKKLTHLSVNANGSIIPKDCVDRNMIKKSLWLKALVAIPKVQPRF 480
           DHIVGLTCGLASCQFRKKLTHLSVNANGSIIPKDCVDRNMIKKSLWLKALVAIPKVQPRF
Sbjct: 421 DHIVGLTCGLASCQFRKKLTHLSVNANGSIIPKDCVDRNMIKKSLWLKALVAIPKVQPRF 480

Query: 481 AIAVWKKYPTMKSLLSVYMDPRKSVHEKEFLLKDLTIEGLLGDDRRLGEICSKRVYRILM 540
           AIA+WKKYPTMKSLLSVYMDP KSVHEKEFLLKDLTIEGLLGDDRRLGEICSKRVYRILM
Sbjct: 481 AIAIWKKYPTMKSLLSVYMDPCKSVHEKEFLLKDLTIEGLLGDDRRLGEICSKRVYRILM 540

Query: 541 AQNGNMRTDDVEEGADLFRSQLS 563
           AQ+G+MRTDDVE+GADLFRSQLS
Sbjct: 541 AQSGSMRTDDVEDGADLFRSQLS 563

BLAST of CsGy6G026350 vs. NCBI nr
Match: XP_008440091.1 (PREDICTED: crossover junction endonuclease EME1B isoform X2 [Cucumis melo])

HSP 1 Score: 1036 bits (2678), Expect = 0.0
Identity = 532/553 (96.20%), Postives = 542/553 (98.01%), Query Frame = 0

Query: 1   MSQPIILSDEEDRYSTPIPIPSKKRRTESDLLPNIKSTVLVLDDDPTPQKSNHNSAASFV 60
           MSQPI+LSDEEDRYSTPIPIPSKKRRTESDLLP+IKSTVLVLDDDPTPQKSNHNSAASFV
Sbjct: 1   MSQPIVLSDEEDRYSTPIPIPSKKRRTESDLLPSIKSTVLVLDDDPTPQKSNHNSAASFV 60

Query: 61  PETPLSPQPSSDVAIVKCTKAGLNLHARDLNSDQKFAGISGLICLESDNESESDSQKKKC 120
           PETPLSPQ SSDVAIVKCTKAGLNLHARDLNSDQKFAGISGLICLESDNESESDSQK+KC
Sbjct: 61  PETPLSPQLSSDVAIVKCTKAGLNLHARDLNSDQKFAGISGLICLESDNESESDSQKRKC 120

Query: 121 EQSEWIGSDCYEMKNIGASLPFDSAGSGSSLGNDDLQEISGDMKHSTFGRDDDIYQVHDY 180
           EQSEWIGSDCYEMKNIGASLPFDSAGSGSS GNDDLQEISGD KHS FGRD DIYQVHDY
Sbjct: 121 EQSEWIGSDCYEMKNIGASLPFDSAGSGSSHGNDDLQEISGDTKHSAFGRDGDIYQVHDY 180

Query: 181 PEEDNVGMEQNDNSMKTTGRKANAEKVTRKKATKDDKISLMEEKKKKKELEKLQKAAQKA 240
           PE+DNVGMEQNDNS+KTTGRKANAEKV RKKATKDDKI LMEEKKKKKELEKLQKAAQKA
Sbjct: 181 PEKDNVGMEQNDNSIKTTGRKANAEKVMRKKATKDDKIRLMEEKKKKKELEKLQKAAQKA 240

Query: 241 EAAEMKKMQKEKQKWEKGKLALKSIVAEIDAKVVELGSIGGHLLTRFAEKGITFRIKSNP 300
           EAAEMKKMQKEKQKWEKGKLALKSIVAEIDAKVVELGSIGGHLLTRFAEKGITFRIKSNP
Sbjct: 241 EAAEMKKMQKEKQKWEKGKLALKSIVAEIDAKVVELGSIGGHLLTRFAEKGITFRIKSNP 300

Query: 301 IERSILWTMNVPEHISASSKGLEISYVLLVYEAENFCELLSKESFMDHVARVQSRYPSHT 360
           IERSILWTMNVPEHISASSKGLEISYVLLVYEAENFCELLSKESFMDHVARVQSRYPSHT
Sbjct: 301 IERSILWTMNVPEHISASSKGLEISYVLLVYEAENFCELLSKESFMDHVARVQSRYPSHT 360

Query: 361 ICYLTNRLMAFINKREQEKYKNLTCSNDWIRPPVEEELAKLTTHFVKVRSRQCVDEAEVA 420
           ICYLTNRLMAFINKREQEKYKN TC  DWIRPPVEEELAKL+THFVKVRSRQCVDEAEVA
Sbjct: 361 ICYLTNRLMAFINKREQEKYKNSTCCTDWIRPPVEEELAKLSTHFVKVRSRQCVDEAEVA 420

Query: 421 DHIVGLTCGLASCQFRKKLTHLSVNANGSIIPKDCVDRNMIKKSLWLKALVAIPKVQPRF 480
           DHIVGLTCGLASCQFRKKLTHLSVNANGSIIPKDCVDRNMIKKSLWLKALVAIPKVQPRF
Sbjct: 421 DHIVGLTCGLASCQFRKKLTHLSVNANGSIIPKDCVDRNMIKKSLWLKALVAIPKVQPRF 480

Query: 481 AIAVWKKYPTMKSLLSVYMDPRKSVHEKEFLLKDLTIEGLLGDDRRLGEICSKRVYRILM 540
           AIA+WKKYPTMKSLLSVYMDP KSVHEKEFLLKDLTIEGLLGDDRRLGEICSKRVYRILM
Sbjct: 481 AIAIWKKYPTMKSLLSVYMDPCKSVHEKEFLLKDLTIEGLLGDDRRLGEICSKRVYRILM 540

Query: 541 AQNGNMRTDDVEE 553
           AQ+G+MRTDDVE+
Sbjct: 541 AQSGSMRTDDVED 553

BLAST of CsGy6G026350 vs. NCBI nr
Match: XP_038880967.1 (crossover junction endonuclease EME1B-like [Benincasa hispida])

HSP 1 Score: 1003 bits (2592), Expect = 0.0
Identity = 516/569 (90.69%), Postives = 539/569 (94.73%), Query Frame = 0

Query: 1   MSQPIILSDEEDRYSTPIPIPSKKRRTESDLLPNIKSTVLVLDDDPTPQKSNHNSAASFV 60
           MSQPI+LSDEEDR STP+PI SKKRRTESD+ P IKSTVLVLDDDPTPQKS+ NSA SFV
Sbjct: 1   MSQPIVLSDEEDRNSTPLPIRSKKRRTESDVFPGIKSTVLVLDDDPTPQKSSRNSAVSFV 60

Query: 61  PETPLSPQPSSDVAIVKCTKAGLNLHARDLNSDQKFAGISGLICLESDNESESDSQKKKC 120
           PETPLSP PSSDVAIVKCTKA LNLHAR L SDQKFAGISGLICLESDNESESDSQK+KC
Sbjct: 61  PETPLSPPPSSDVAIVKCTKARLNLHARVLTSDQKFAGISGLICLESDNESESDSQKEKC 120

Query: 121 EQSEWIGSDCYEMKNIGASLPFDSAGSGSSLGNDDLQEISGDMKHSTFGRDDDIYQVHDY 180
             SEWIGSDCYEMKNIG++  FDSAGSG SLGNDDLQEISGD KHSTFG+DDDIYQVHDY
Sbjct: 121 GPSEWIGSDCYEMKNIGSTFQFDSAGSGPSLGNDDLQEISGDTKHSTFGQDDDIYQVHDY 180

Query: 181 PEEDNVGMEQNDNSMKTTGRKANAEK------VTRKKATKDDKISLMEEKKKKKELEKLQ 240
           PE+++VGMEQNDNS+K TGRKANAEK      V RKKATKDDKI LMEEKKKKKELEKLQ
Sbjct: 181 PEKESVGMEQNDNSIKATGRKANAEKNKSIDKVMRKKATKDDKIRLMEEKKKKKELEKLQ 240

Query: 241 KAAQKAEAAEMKKMQKEKQKWEKGKLALKSIVAEIDAKVVELGSIGGHLLTRFAEKGITF 300
           KAAQKAEAAEMKKMQKEKQKWEKGKLALKSIVAEIDAKVVELGS+GGHLLTRFAEKGITF
Sbjct: 241 KAAQKAEAAEMKKMQKEKQKWEKGKLALKSIVAEIDAKVVELGSVGGHLLTRFAEKGITF 300

Query: 301 RIKSNPIERSILWTMNVPEHISASSKGLEISYVLLVYEAENFCELLSKESFMDHVARVQS 360
           RIKSNPIERSILWTMNVPEHIS SSKGLEISY+LLVYEAENFCELLSKESFMDHVARV+S
Sbjct: 301 RIKSNPIERSILWTMNVPEHISESSKGLEISYILLVYEAENFCELLSKESFMDHVARVRS 360

Query: 361 RYPSHTICYLTNRLMAFINKREQEKYKNLTCSNDWIRPPVEEELAKLTTHFVKVRSRQCV 420
            YPS+TICYLTNRLMAFINKR+QEKYKN  CSNDWIRPPVEEELAKL+THFVKVRSRQCV
Sbjct: 361 HYPSYTICYLTNRLMAFINKRDQEKYKNSACSNDWIRPPVEEELAKLSTHFVKVRSRQCV 420

Query: 421 DEAEVADHIVGLTCGLASCQFRKKLTHLSVNANGSIIPKDCVDRNMIKKSLWLKALVAIP 480
           DEAEVADHIVGLTCGLASCQFRKKLTHLSVNANGSIIPKDCVDRN+IKKSLWLKALVAIP
Sbjct: 421 DEAEVADHIVGLTCGLASCQFRKKLTHLSVNANGSIIPKDCVDRNVIKKSLWLKALVAIP 480

Query: 481 KVQPRFAIAVWKKYPTMKSLLSVYMDPRKSVHEKEFLLKDLTIEGLLGDDRRLGEICSKR 540
           KVQPRFA+A+WKKYPTMKSLLSVYMDP KSVHEKEFLLKDLTIEGLLGDDRRLGE+CSKR
Sbjct: 481 KVQPRFAVAIWKKYPTMKSLLSVYMDPCKSVHEKEFLLKDLTIEGLLGDDRRLGEMCSKR 540

Query: 541 VYRILMAQNGNMRTDDVEEGADLFRSQLS 563
           VYRILMAQNG+MRTDDVEEGADLFRSQLS
Sbjct: 541 VYRILMAQNGSMRTDDVEEGADLFRSQLS 569

BLAST of CsGy6G026350 vs. NCBI nr
Match: TYK12984.1 (crossover junction endonuclease EME1B isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 992 bits (2565), Expect = 0.0
Identity = 514/563 (91.30%), Postives = 524/563 (93.07%), Query Frame = 0

Query: 1   MSQPIILSDEEDRYSTPIPIPSKKRRTESDLLPNIKSTVLVLDDDPTPQKSNHNSAASFV 60
           MSQPI+LSDEEDRYSTPIPIPSKKRRTESDLLP+IKSTVLVLDDDPTPQKSNHNSAASFV
Sbjct: 1   MSQPIVLSDEEDRYSTPIPIPSKKRRTESDLLPSIKSTVLVLDDDPTPQKSNHNSAASFV 60

Query: 61  PETPLSPQPSSDVAIVKCTKAGLNLHARDLNSDQKFAGISGLICLESDNESESDSQKKKC 120
           PETPLSPQPSSDVAIVKCTKAGLNLHARDLNSDQKFAGISGLICLESDNESESDSQK+KC
Sbjct: 61  PETPLSPQPSSDVAIVKCTKAGLNLHARDLNSDQKFAGISGLICLESDNESESDSQKRKC 120

Query: 121 EQSEWIGSDCYEMKNIGASLPFDSAGSGSSLGNDDLQEISGDMKHSTFGRDDDIYQVHDY 180
           EQSEWIGSDCYEMKNIGASLPFDSAGSGSS GNDDLQEISGD KHS FGRD DIYQVHDY
Sbjct: 121 EQSEWIGSDCYEMKNIGASLPFDSAGSGSSHGNDDLQEISGDTKHSAFGRDGDIYQVHDY 180

Query: 181 PEEDNVGMEQNDNSMKTTGRKANAEKVTRKKATKDDKISLMEEKKKKKELEKLQKAAQKA 240
           PE+DNVGMEQNDNS+KTTGRKANAEK                       LEKLQKAAQKA
Sbjct: 181 PEKDNVGMEQNDNSIKTTGRKANAEK-----------------------LEKLQKAAQKA 240

Query: 241 EAAEMKKMQKEKQKWEKGKLALKSIVAEIDAKVVELGSIGGHLLTRFAEKGITFRIKSNP 300
           EAAEMKKMQKEKQKWEKGKLALKSIVAEIDAKVVELGSIGGHLLTRFAEKGITFRIKSNP
Sbjct: 241 EAAEMKKMQKEKQKWEKGKLALKSIVAEIDAKVVELGSIGGHLLTRFAEKGITFRIKSNP 300

Query: 301 IERSILWTMNVPEHISASSKGLEISYVLLVYEAENFCELLSKESFMDHVARVQSRYPSHT 360
           IERSILWTMNVPEHISASSKGLEISYVLLVYEAENFCELLSKESFMDHVARVQSRYPSHT
Sbjct: 301 IERSILWTMNVPEHISASSKGLEISYVLLVYEAENFCELLSKESFMDHVARVQSRYPSHT 360

Query: 361 ICYLTNRLMAFINKREQEKYKNLTCSNDWIRPPVEEELAKLTTHFVKVRSRQCVDEAEVA 420
           ICYLTNR        EQEKYKN TC  DWIRPPVEEELAKL+THFVKVRSRQCVDEAEVA
Sbjct: 361 ICYLTNR--------EQEKYKNSTCCTDWIRPPVEEELAKLSTHFVKVRSRQCVDEAEVA 420

Query: 421 DHIVGLTCGLASCQFRKKLTHLSVNANGSIIPKDCVDRNMIKKSLWLKALVAIPKVQPRF 480
           DHIVGLTCGLASCQFRKKLTHLSVNANGSIIPKDCVDRNMIKKSLWLKALVAIPKVQPRF
Sbjct: 421 DHIVGLTCGLASCQFRKKLTHLSVNANGSIIPKDCVDRNMIKKSLWLKALVAIPKVQPRF 480

Query: 481 AIAVWKKYPTMKSLLSVYMDPRKSVHEKEFLLKDLTIEGLLGDDRRLGEICSKRVYRILM 540
           AIA+WKKYPTMKSLLSVYMDP KSVHEKEFLLKDLTIEGLLGDDRRLGEICSKRVYRILM
Sbjct: 481 AIAIWKKYPTMKSLLSVYMDPCKSVHEKEFLLKDLTIEGLLGDDRRLGEICSKRVYRILM 532

Query: 541 AQNGNMRTDDVEEGADLFRSQLS 563
           AQ+G+MRTDDVE+GADLFRSQLS
Sbjct: 541 AQSGSMRTDDVEDGADLFRSQLS 532

BLAST of CsGy6G026350 vs. ExPASy TrEMBL
Match: A0A0A0KHY1 (ERCC4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G486820 PE=3 SV=1)

HSP 1 Score: 1096 bits (2834), Expect = 0.0
Identity = 563/563 (100.00%), Postives = 563/563 (100.00%), Query Frame = 0

Query: 1   MSQPIILSDEEDRYSTPIPIPSKKRRTESDLLPNIKSTVLVLDDDPTPQKSNHNSAASFV 60
           MSQPIILSDEEDRYSTPIPIPSKKRRTESDLLPNIKSTVLVLDDDPTPQKSNHNSAASFV
Sbjct: 1   MSQPIILSDEEDRYSTPIPIPSKKRRTESDLLPNIKSTVLVLDDDPTPQKSNHNSAASFV 60

Query: 61  PETPLSPQPSSDVAIVKCTKAGLNLHARDLNSDQKFAGISGLICLESDNESESDSQKKKC 120
           PETPLSPQPSSDVAIVKCTKAGLNLHARDLNSDQKFAGISGLICLESDNESESDSQKKKC
Sbjct: 61  PETPLSPQPSSDVAIVKCTKAGLNLHARDLNSDQKFAGISGLICLESDNESESDSQKKKC 120

Query: 121 EQSEWIGSDCYEMKNIGASLPFDSAGSGSSLGNDDLQEISGDMKHSTFGRDDDIYQVHDY 180
           EQSEWIGSDCYEMKNIGASLPFDSAGSGSSLGNDDLQEISGDMKHSTFGRDDDIYQVHDY
Sbjct: 121 EQSEWIGSDCYEMKNIGASLPFDSAGSGSSLGNDDLQEISGDMKHSTFGRDDDIYQVHDY 180

Query: 181 PEEDNVGMEQNDNSMKTTGRKANAEKVTRKKATKDDKISLMEEKKKKKELEKLQKAAQKA 240
           PEEDNVGMEQNDNSMKTTGRKANAEKVTRKKATKDDKISLMEEKKKKKELEKLQKAAQKA
Sbjct: 181 PEEDNVGMEQNDNSMKTTGRKANAEKVTRKKATKDDKISLMEEKKKKKELEKLQKAAQKA 240

Query: 241 EAAEMKKMQKEKQKWEKGKLALKSIVAEIDAKVVELGSIGGHLLTRFAEKGITFRIKSNP 300
           EAAEMKKMQKEKQKWEKGKLALKSIVAEIDAKVVELGSIGGHLLTRFAEKGITFRIKSNP
Sbjct: 241 EAAEMKKMQKEKQKWEKGKLALKSIVAEIDAKVVELGSIGGHLLTRFAEKGITFRIKSNP 300

Query: 301 IERSILWTMNVPEHISASSKGLEISYVLLVYEAENFCELLSKESFMDHVARVQSRYPSHT 360
           IERSILWTMNVPEHISASSKGLEISYVLLVYEAENFCELLSKESFMDHVARVQSRYPSHT
Sbjct: 301 IERSILWTMNVPEHISASSKGLEISYVLLVYEAENFCELLSKESFMDHVARVQSRYPSHT 360

Query: 361 ICYLTNRLMAFINKREQEKYKNLTCSNDWIRPPVEEELAKLTTHFVKVRSRQCVDEAEVA 420
           ICYLTNRLMAFINKREQEKYKNLTCSNDWIRPPVEEELAKLTTHFVKVRSRQCVDEAEVA
Sbjct: 361 ICYLTNRLMAFINKREQEKYKNLTCSNDWIRPPVEEELAKLTTHFVKVRSRQCVDEAEVA 420

Query: 421 DHIVGLTCGLASCQFRKKLTHLSVNANGSIIPKDCVDRNMIKKSLWLKALVAIPKVQPRF 480
           DHIVGLTCGLASCQFRKKLTHLSVNANGSIIPKDCVDRNMIKKSLWLKALVAIPKVQPRF
Sbjct: 421 DHIVGLTCGLASCQFRKKLTHLSVNANGSIIPKDCVDRNMIKKSLWLKALVAIPKVQPRF 480

Query: 481 AIAVWKKYPTMKSLLSVYMDPRKSVHEKEFLLKDLTIEGLLGDDRRLGEICSKRVYRILM 540
           AIAVWKKYPTMKSLLSVYMDPRKSVHEKEFLLKDLTIEGLLGDDRRLGEICSKRVYRILM
Sbjct: 481 AIAVWKKYPTMKSLLSVYMDPRKSVHEKEFLLKDLTIEGLLGDDRRLGEICSKRVYRILM 540

Query: 541 AQNGNMRTDDVEEGADLFRSQLS 563
           AQNGNMRTDDVEEGADLFRSQLS
Sbjct: 541 AQNGNMRTDDVEEGADLFRSQLS 563

BLAST of CsGy6G026350 vs. ExPASy TrEMBL
Match: A0A1S3B119 (crossover junction endonuclease EME1B isoform X1 OS=Cucumis melo OX=3656 GN=LOC103484673 PE=3 SV=1)

HSP 1 Score: 1055 bits (2728), Expect = 0.0
Identity = 542/563 (96.27%), Postives = 552/563 (98.05%), Query Frame = 0

Query: 1   MSQPIILSDEEDRYSTPIPIPSKKRRTESDLLPNIKSTVLVLDDDPTPQKSNHNSAASFV 60
           MSQPI+LSDEEDRYSTPIPIPSKKRRTESDLLP+IKSTVLVLDDDPTPQKSNHNSAASFV
Sbjct: 1   MSQPIVLSDEEDRYSTPIPIPSKKRRTESDLLPSIKSTVLVLDDDPTPQKSNHNSAASFV 60

Query: 61  PETPLSPQPSSDVAIVKCTKAGLNLHARDLNSDQKFAGISGLICLESDNESESDSQKKKC 120
           PETPLSPQ SSDVAIVKCTKAGLNLHARDLNSDQKFAGISGLICLESDNESESDSQK+KC
Sbjct: 61  PETPLSPQLSSDVAIVKCTKAGLNLHARDLNSDQKFAGISGLICLESDNESESDSQKRKC 120

Query: 121 EQSEWIGSDCYEMKNIGASLPFDSAGSGSSLGNDDLQEISGDMKHSTFGRDDDIYQVHDY 180
           EQSEWIGSDCYEMKNIGASLPFDSAGSGSS GNDDLQEISGD KHS FGRD DIYQVHDY
Sbjct: 121 EQSEWIGSDCYEMKNIGASLPFDSAGSGSSHGNDDLQEISGDTKHSAFGRDGDIYQVHDY 180

Query: 181 PEEDNVGMEQNDNSMKTTGRKANAEKVTRKKATKDDKISLMEEKKKKKELEKLQKAAQKA 240
           PE+DNVGMEQNDNS+KTTGRKANAEKV RKKATKDDKI LMEEKKKKKELEKLQKAAQKA
Sbjct: 181 PEKDNVGMEQNDNSIKTTGRKANAEKVMRKKATKDDKIRLMEEKKKKKELEKLQKAAQKA 240

Query: 241 EAAEMKKMQKEKQKWEKGKLALKSIVAEIDAKVVELGSIGGHLLTRFAEKGITFRIKSNP 300
           EAAEMKKMQKEKQKWEKGKLALKSIVAEIDAKVVELGSIGGHLLTRFAEKGITFRIKSNP
Sbjct: 241 EAAEMKKMQKEKQKWEKGKLALKSIVAEIDAKVVELGSIGGHLLTRFAEKGITFRIKSNP 300

Query: 301 IERSILWTMNVPEHISASSKGLEISYVLLVYEAENFCELLSKESFMDHVARVQSRYPSHT 360
           IERSILWTMNVPEHISASSKGLEISYVLLVYEAENFCELLSKESFMDHVARVQSRYPSHT
Sbjct: 301 IERSILWTMNVPEHISASSKGLEISYVLLVYEAENFCELLSKESFMDHVARVQSRYPSHT 360

Query: 361 ICYLTNRLMAFINKREQEKYKNLTCSNDWIRPPVEEELAKLTTHFVKVRSRQCVDEAEVA 420
           ICYLTNRLMAFINKREQEKYKN TC  DWIRPPVEEELAKL+THFVKVRSRQCVDEAEVA
Sbjct: 361 ICYLTNRLMAFINKREQEKYKNSTCCTDWIRPPVEEELAKLSTHFVKVRSRQCVDEAEVA 420

Query: 421 DHIVGLTCGLASCQFRKKLTHLSVNANGSIIPKDCVDRNMIKKSLWLKALVAIPKVQPRF 480
           DHIVGLTCGLASCQFRKKLTHLSVNANGSIIPKDCVDRNMIKKSLWLKALVAIPKVQPRF
Sbjct: 421 DHIVGLTCGLASCQFRKKLTHLSVNANGSIIPKDCVDRNMIKKSLWLKALVAIPKVQPRF 480

Query: 481 AIAVWKKYPTMKSLLSVYMDPRKSVHEKEFLLKDLTIEGLLGDDRRLGEICSKRVYRILM 540
           AIA+WKKYPTMKSLLSVYMDP KSVHEKEFLLKDLTIEGLLGDDRRLGEICSKRVYRILM
Sbjct: 481 AIAIWKKYPTMKSLLSVYMDPCKSVHEKEFLLKDLTIEGLLGDDRRLGEICSKRVYRILM 540

Query: 541 AQNGNMRTDDVEEGADLFRSQLS 563
           AQ+G+MRTDDVE+GADLFRSQLS
Sbjct: 541 AQSGSMRTDDVEDGADLFRSQLS 563

BLAST of CsGy6G026350 vs. ExPASy TrEMBL
Match: A0A1S3AZW5 (crossover junction endonuclease EME1B isoform X2 OS=Cucumis melo OX=3656 GN=LOC103484673 PE=3 SV=1)

HSP 1 Score: 1036 bits (2678), Expect = 0.0
Identity = 532/553 (96.20%), Postives = 542/553 (98.01%), Query Frame = 0

Query: 1   MSQPIILSDEEDRYSTPIPIPSKKRRTESDLLPNIKSTVLVLDDDPTPQKSNHNSAASFV 60
           MSQPI+LSDEEDRYSTPIPIPSKKRRTESDLLP+IKSTVLVLDDDPTPQKSNHNSAASFV
Sbjct: 1   MSQPIVLSDEEDRYSTPIPIPSKKRRTESDLLPSIKSTVLVLDDDPTPQKSNHNSAASFV 60

Query: 61  PETPLSPQPSSDVAIVKCTKAGLNLHARDLNSDQKFAGISGLICLESDNESESDSQKKKC 120
           PETPLSPQ SSDVAIVKCTKAGLNLHARDLNSDQKFAGISGLICLESDNESESDSQK+KC
Sbjct: 61  PETPLSPQLSSDVAIVKCTKAGLNLHARDLNSDQKFAGISGLICLESDNESESDSQKRKC 120

Query: 121 EQSEWIGSDCYEMKNIGASLPFDSAGSGSSLGNDDLQEISGDMKHSTFGRDDDIYQVHDY 180
           EQSEWIGSDCYEMKNIGASLPFDSAGSGSS GNDDLQEISGD KHS FGRD DIYQVHDY
Sbjct: 121 EQSEWIGSDCYEMKNIGASLPFDSAGSGSSHGNDDLQEISGDTKHSAFGRDGDIYQVHDY 180

Query: 181 PEEDNVGMEQNDNSMKTTGRKANAEKVTRKKATKDDKISLMEEKKKKKELEKLQKAAQKA 240
           PE+DNVGMEQNDNS+KTTGRKANAEKV RKKATKDDKI LMEEKKKKKELEKLQKAAQKA
Sbjct: 181 PEKDNVGMEQNDNSIKTTGRKANAEKVMRKKATKDDKIRLMEEKKKKKELEKLQKAAQKA 240

Query: 241 EAAEMKKMQKEKQKWEKGKLALKSIVAEIDAKVVELGSIGGHLLTRFAEKGITFRIKSNP 300
           EAAEMKKMQKEKQKWEKGKLALKSIVAEIDAKVVELGSIGGHLLTRFAEKGITFRIKSNP
Sbjct: 241 EAAEMKKMQKEKQKWEKGKLALKSIVAEIDAKVVELGSIGGHLLTRFAEKGITFRIKSNP 300

Query: 301 IERSILWTMNVPEHISASSKGLEISYVLLVYEAENFCELLSKESFMDHVARVQSRYPSHT 360
           IERSILWTMNVPEHISASSKGLEISYVLLVYEAENFCELLSKESFMDHVARVQSRYPSHT
Sbjct: 301 IERSILWTMNVPEHISASSKGLEISYVLLVYEAENFCELLSKESFMDHVARVQSRYPSHT 360

Query: 361 ICYLTNRLMAFINKREQEKYKNLTCSNDWIRPPVEEELAKLTTHFVKVRSRQCVDEAEVA 420
           ICYLTNRLMAFINKREQEKYKN TC  DWIRPPVEEELAKL+THFVKVRSRQCVDEAEVA
Sbjct: 361 ICYLTNRLMAFINKREQEKYKNSTCCTDWIRPPVEEELAKLSTHFVKVRSRQCVDEAEVA 420

Query: 421 DHIVGLTCGLASCQFRKKLTHLSVNANGSIIPKDCVDRNMIKKSLWLKALVAIPKVQPRF 480
           DHIVGLTCGLASCQFRKKLTHLSVNANGSIIPKDCVDRNMIKKSLWLKALVAIPKVQPRF
Sbjct: 421 DHIVGLTCGLASCQFRKKLTHLSVNANGSIIPKDCVDRNMIKKSLWLKALVAIPKVQPRF 480

Query: 481 AIAVWKKYPTMKSLLSVYMDPRKSVHEKEFLLKDLTIEGLLGDDRRLGEICSKRVYRILM 540
           AIA+WKKYPTMKSLLSVYMDP KSVHEKEFLLKDLTIEGLLGDDRRLGEICSKRVYRILM
Sbjct: 481 AIAIWKKYPTMKSLLSVYMDPCKSVHEKEFLLKDLTIEGLLGDDRRLGEICSKRVYRILM 540

Query: 541 AQNGNMRTDDVEE 553
           AQ+G+MRTDDVE+
Sbjct: 541 AQSGSMRTDDVED 553

BLAST of CsGy6G026350 vs. ExPASy TrEMBL
Match: A0A5D3CPM4 (Crossover junction endonuclease EME1B isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G005810 PE=3 SV=1)

HSP 1 Score: 992 bits (2565), Expect = 0.0
Identity = 514/563 (91.30%), Postives = 524/563 (93.07%), Query Frame = 0

Query: 1   MSQPIILSDEEDRYSTPIPIPSKKRRTESDLLPNIKSTVLVLDDDPTPQKSNHNSAASFV 60
           MSQPI+LSDEEDRYSTPIPIPSKKRRTESDLLP+IKSTVLVLDDDPTPQKSNHNSAASFV
Sbjct: 1   MSQPIVLSDEEDRYSTPIPIPSKKRRTESDLLPSIKSTVLVLDDDPTPQKSNHNSAASFV 60

Query: 61  PETPLSPQPSSDVAIVKCTKAGLNLHARDLNSDQKFAGISGLICLESDNESESDSQKKKC 120
           PETPLSPQPSSDVAIVKCTKAGLNLHARDLNSDQKFAGISGLICLESDNESESDSQK+KC
Sbjct: 61  PETPLSPQPSSDVAIVKCTKAGLNLHARDLNSDQKFAGISGLICLESDNESESDSQKRKC 120

Query: 121 EQSEWIGSDCYEMKNIGASLPFDSAGSGSSLGNDDLQEISGDMKHSTFGRDDDIYQVHDY 180
           EQSEWIGSDCYEMKNIGASLPFDSAGSGSS GNDDLQEISGD KHS FGRD DIYQVHDY
Sbjct: 121 EQSEWIGSDCYEMKNIGASLPFDSAGSGSSHGNDDLQEISGDTKHSAFGRDGDIYQVHDY 180

Query: 181 PEEDNVGMEQNDNSMKTTGRKANAEKVTRKKATKDDKISLMEEKKKKKELEKLQKAAQKA 240
           PE+DNVGMEQNDNS+KTTGRKANAEK                       LEKLQKAAQKA
Sbjct: 181 PEKDNVGMEQNDNSIKTTGRKANAEK-----------------------LEKLQKAAQKA 240

Query: 241 EAAEMKKMQKEKQKWEKGKLALKSIVAEIDAKVVELGSIGGHLLTRFAEKGITFRIKSNP 300
           EAAEMKKMQKEKQKWEKGKLALKSIVAEIDAKVVELGSIGGHLLTRFAEKGITFRIKSNP
Sbjct: 241 EAAEMKKMQKEKQKWEKGKLALKSIVAEIDAKVVELGSIGGHLLTRFAEKGITFRIKSNP 300

Query: 301 IERSILWTMNVPEHISASSKGLEISYVLLVYEAENFCELLSKESFMDHVARVQSRYPSHT 360
           IERSILWTMNVPEHISASSKGLEISYVLLVYEAENFCELLSKESFMDHVARVQSRYPSHT
Sbjct: 301 IERSILWTMNVPEHISASSKGLEISYVLLVYEAENFCELLSKESFMDHVARVQSRYPSHT 360

Query: 361 ICYLTNRLMAFINKREQEKYKNLTCSNDWIRPPVEEELAKLTTHFVKVRSRQCVDEAEVA 420
           ICYLTNR        EQEKYKN TC  DWIRPPVEEELAKL+THFVKVRSRQCVDEAEVA
Sbjct: 361 ICYLTNR--------EQEKYKNSTCCTDWIRPPVEEELAKLSTHFVKVRSRQCVDEAEVA 420

Query: 421 DHIVGLTCGLASCQFRKKLTHLSVNANGSIIPKDCVDRNMIKKSLWLKALVAIPKVQPRF 480
           DHIVGLTCGLASCQFRKKLTHLSVNANGSIIPKDCVDRNMIKKSLWLKALVAIPKVQPRF
Sbjct: 421 DHIVGLTCGLASCQFRKKLTHLSVNANGSIIPKDCVDRNMIKKSLWLKALVAIPKVQPRF 480

Query: 481 AIAVWKKYPTMKSLLSVYMDPRKSVHEKEFLLKDLTIEGLLGDDRRLGEICSKRVYRILM 540
           AIA+WKKYPTMKSLLSVYMDP KSVHEKEFLLKDLTIEGLLGDDRRLGEICSKRVYRILM
Sbjct: 481 AIAIWKKYPTMKSLLSVYMDPCKSVHEKEFLLKDLTIEGLLGDDRRLGEICSKRVYRILM 532

Query: 541 AQNGNMRTDDVEEGADLFRSQLS 563
           AQ+G+MRTDDVE+GADLFRSQLS
Sbjct: 541 AQSGSMRTDDVEDGADLFRSQLS 532

BLAST of CsGy6G026350 vs. ExPASy TrEMBL
Match: A0A6J1IIZ2 (crossover junction endonuclease EME1B isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111477883 PE=3 SV=1)

HSP 1 Score: 959 bits (2479), Expect = 0.0
Identity = 494/568 (86.97%), Postives = 523/568 (92.08%), Query Frame = 0

Query: 1   MSQPIILSDEEDRYSTPIPIPSKKRRTESDLLPNIKSTVLVLDDDPTPQKSNHNSAASFV 60
           MS+PI+LSDEEDRYSTP+PIPSKKRRTES   P+IKSTVLVLDDDPTPQKS  NSAASFV
Sbjct: 1   MSRPIVLSDEEDRYSTPLPIPSKKRRTESHAFPSIKSTVLVLDDDPTPQKSGSNSAASFV 60

Query: 61  PETPLSPQPSSDVAIVKCTKAGLNLHARDLNSDQKFAGISGLICLESDNESESDSQKKKC 120
           PETPLSP PSSDV IVKCTK  L+L ARDLNSD KFAGISGLICLESDNESESDS KK C
Sbjct: 61  PETPLSPPPSSDVVIVKCTKVPLDLPARDLNSDHKFAGISGLICLESDNESESDSLKKMC 120

Query: 121 EQSEWIGSDCYEMKNIGASLPFDSAGSGSSLGNDDLQEISGDMKHSTFGRDDDIYQVHDY 180
             SEWIGSDC +MKN+G++  FDSAGSG S+GNDDL EISGD KHST GRDD IYQVHDY
Sbjct: 121 GPSEWIGSDCDDMKNLGSTSKFDSAGSGPSIGNDDLHEISGDTKHSTVGRDDYIYQVHDY 180

Query: 181 PEEDNVGMEQNDNSMKTTGRKANAEK------VTRKKATKDDKISLMEEKKKKKELEKLQ 240
           PE++NVGMEQNDN++K TGRK++AEK        RKKATKDDKI LMEEKKKKKELEKLQ
Sbjct: 181 PEKENVGMEQNDNTIKPTGRKSDAEKNKNVDKAMRKKATKDDKIRLMEEKKKKKELEKLQ 240

Query: 241 KAAQKAEAAEMKKMQKEKQKWEKGKLALKSIVAEIDAKVVELGSIGGHLLTRFAEKGITF 300
           KAAQKAEAAEM+KMQKEKQKWEKGKLALKSIVAEIDAKVVELGS+GGHLLTRFAEKGITF
Sbjct: 241 KAAQKAEAAEMRKMQKEKQKWEKGKLALKSIVAEIDAKVVELGSVGGHLLTRFAEKGITF 300

Query: 301 RIKSNPIERSILWTMNVPEHISASSKGLEISYVLLVYEAENFCELLSKESFMDHVARVQS 360
           RIKSNPIERSI+WTMN PEHIS S KGLEI+YVLL+YEAENFCELLSKES + HVARVQS
Sbjct: 301 RIKSNPIERSIVWTMNAPEHISESFKGLEIAYVLLMYEAENFCELLSKESLVHHVARVQS 360

Query: 361 RYPSHTICYLTNRLMAFINKREQEKYKNLTCSNDWIRPPVEEELAKLTTHFVKVRSRQCV 420
            YPSHTICYLTNRLMAFINKREQEKYKN   SN WIRPPVEEELAKLTTHFVKVRSRQCV
Sbjct: 361 HYPSHTICYLTNRLMAFINKREQEKYKNSASSNGWIRPPVEEELAKLTTHFVKVRSRQCV 420

Query: 421 DEAEVADHIVGLTCGLASCQFRKKLTHLSVNANGSIIPKDCVDRNMIKKSLWLKALVAIP 480
           DEAEVADHIVGLTC LASCQFRKKLTHLSVNANGSIIPKDC+DRNMIKKSLWLKALVAIP
Sbjct: 421 DEAEVADHIVGLTCSLASCQFRKKLTHLSVNANGSIIPKDCIDRNMIKKSLWLKALVAIP 480

Query: 481 KVQPRFAIAVWKKYPTMKSLLSVYMDPRKSVHEKEFLLKDLTIEGLLGDDRRLGEICSKR 540
           KVQPRFA+A+WKKYPTMKSLLSVYMDP KSVHEKEFLLKDLTIEGLLGDDRRLGE+CSKR
Sbjct: 481 KVQPRFAVAIWKKYPTMKSLLSVYMDPCKSVHEKEFLLKDLTIEGLLGDDRRLGEMCSKR 540

Query: 541 VYRILMAQNGNMRTDDVEEGADLFRSQL 562
           VYRILMAQ+G+MRTDDVEEGAD+FRSQL
Sbjct: 541 VYRILMAQSGSMRTDDVEEGADMFRSQL 568

BLAST of CsGy6G026350 vs. TAIR 10
Match: AT2G22140.1 (essential meiotic endonuclease 1B )

HSP 1 Score: 497.7 bits (1280), Expect = 1.3e-140
Identity = 289/565 (51.15%), Postives = 393/565 (69.56%), Query Frame = 0

Query: 1   MSQPIILSDEEDRYSTPIPIPSKKRRTESDLLPNIKSTVLVLDDDPTPQKSNHNSAAS-- 60
           M+  I++SD ED+ +TP+P  SK+ R          S +L+ D DPTPQK    S+ +  
Sbjct: 1   MNDHILISDGEDQ-TTPLPSLSKRARKYP------ISAILISDSDPTPQKQPPESSFTPI 60

Query: 61  FVPETPLSPQPSSDVAIVKCTKAGLNLHARDLNSDQKFAGISGLICLESDNESESDSQKK 120
           FVPETPL    S D ++VKC+       A   N + KF   SG   +  D+E E   + +
Sbjct: 61  FVPETPL----SDDFSVVKCS---FGSRALASNREDKF---SGKRIISLDSEFEDSPRPE 120

Query: 121 KCEQSEWIGSDCYEMKNIGASLPFDSAGSGSSLGNDDLQEISGDMKHSTF--GRDDDIYQ 180
             +++E + +   E +    +   ++    + +   +L + +  M   +F     +D  +
Sbjct: 121 TSKKNESVLAGLREPRFGLEAETSEAYYKNTRIPETNLDDDTSWMHEVSFRSSPTNDTIE 180

Query: 181 VHDYPEEDNVGMEQ--NDNSMKTTGRKANAEKVTRKKATKDDKISLMEEKKKKKELEKLQ 240
           V    E++++ +E+      ++TT      E + +K+ +K+DK S MEEKK +KE E+L+
Sbjct: 181 VVSDQEKEDISVEKIGRKKKIRTTTLPVPGEALPKKRQSKEDKTSAMEEKKLRKEQERLE 240

Query: 241 KAAQKAEAAEMKKMQKEKQKWEKGKLALKSIVAEIDAKVVELGSIGGHLLTRFAEKGITF 300
           KAA KAE AE K+++KEK+KWEKGKLALKSIVAEID KV+E GSIGG LL+RF+EKGIT 
Sbjct: 241 KAASKAEEAERKRLEKEKKKWEKGKLALKSIVAEIDTKVLE-GSIGGLLLSRFSEKGITI 300

Query: 301 RIKSNPIERSILWTMNVPEHIS-ASSKGLEISYVLLVYEAENFCELLSKESFMDHVARVQ 360
            +  NPIERSI+WTM +PE I+    +G +I Y+LLVY+AE FC L++   F++ ++RVQ
Sbjct: 301 HVGPNPIERSIVWTMTIPEDIAPLFPQGPKIPYLLLVYDAEEFCNLVANGKFLEIISRVQ 360

Query: 361 SRYPSHTICYLTNRLMAFINKREQEKYKNLTCSNDWIRPPVEEELAKLTTHFVKVRSRQC 420
            RYPS+T+C LTN+LM+++ KRE+E+YKN     +W RPP++E LAKLTTH+VKV SR C
Sbjct: 361 DRYPSYTVCCLTNKLMSYVKKREKEEYKN---PGNWRRPPIDEVLAKLTTHYVKVHSRHC 420

Query: 421 VDEAEVADHIVGLTCGLASCQFRKKLTHLSVNANGSIIPKDCVDRNMIKKSLWLKALVAI 480
           VDEAEVA+HIVGLT  LASCQFRKKLT LSV+ANG+++ KD VD+++IKKS WLKALVAI
Sbjct: 421 VDEAEVAEHIVGLTSSLASCQFRKKLTMLSVSANGALVSKDSVDKHLIKKSPWLKALVAI 480

Query: 481 PKVQPRFAIAVWKKYPTMKSLLSVYMDPRKSVHEKEFLLKDLTIEGLLGDDRRLGEICSK 540
           PKVQPR+A+AVWKKYP+MKSLL VYMD  KSVHEKEFLLKDL +EGL+G D RLGEICSK
Sbjct: 481 PKVQPRYALAVWKKYPSMKSLLKVYMDRNKSVHEKEFLLKDLKVEGLVGGDIRLGEICSK 540

Query: 541 RVYRILMAQNGNMRTDDVEEGADLF 559
           R+YR+LM+ +G ++TDDVE GA  F
Sbjct: 541 RIYRVLMSHDGAIKTDDVENGAAFF 544

BLAST of CsGy6G026350 vs. TAIR 10
Match: AT2G21800.1 (essential meiotic endonuclease 1A )

HSP 1 Score: 424.1 bits (1089), Expect = 1.8e-118
Identity = 262/565 (46.37%), Postives = 370/565 (65.49%), Query Frame = 0

Query: 1   MSQPIILSDEEDRYSTPIPIPSKKRRTESDLLPNIKSTVLVLDDDPTPQKSNHNSAAS-- 60
           MS  I++SD ED  + P    ++K RT +D         L LD +P+ QK    SA++  
Sbjct: 1   MSDFILISDGEDEATPPPSKRARKNRTPTD---------LNLDTEPSLQKQPPGSASTPF 60

Query: 61  FVPETPLSPQPSSDVAIVKCTKAGLNLHARDLNSDQKFAGISGLICLESDNESESDSQKK 120
           F+ ETPL    S DV ++K +  G    A     +  F G   +I LESD+E     +  
Sbjct: 61  FLDETPL----SDDVTVLK-SSFGSGTGASS-GRENNFFG-KRVISLESDSEDSPGPESS 120

Query: 121 KCEQSEWIGSDCYEMKNIGASLPFDSAGSGSSLGNDDLQEISGDMKHSTFGRDDDIYQVH 180
           K  + E + +D ++       L F   GS  +  +DD   +      S+  +  D  +V 
Sbjct: 121 K--KYEPVYTDSWKKP---CRLEF---GSSDANSDDDPSWMRRASFQSSLSK--DAIEVD 180

Query: 181 DYPEEDNVGMEQNDNSMKTTGRKA---NAEKVTRKKATKDDKISLMEEKKKKKELEKLQK 240
              E+++ G+E+     +T   K+   +A+ + +KK +KD+K    EEKK +KE EKLQK
Sbjct: 181 SDHEKEDTGVEKMGRKKQTITSKSTSLSADSLPKKKMSKDEKTRAAEEKKLQKEQEKLQK 240

Query: 241 AAQKAEAAEMKKMQKEKQKWEKGK-LALKSIVAEIDAKVVELGSIGGHLLTRFAEKGITF 300
           AA KAE AE KK+++EKQKW K K  ALK IVA ID KV+E GS GG L++   EK IT+
Sbjct: 241 AASKAEDAEHKKLEREKQKWAKEKDKALKCIVAWIDNKVLE-GSFGGLLISGLKEKCITY 300

Query: 301 RIKSNPIERSILWTMNVPEHISAS-SKGLEISYVLLVYEAENFCELLSKESFMDHVARVQ 360
            + +NPI+RSI+WTM +PE I+ S   G +I YVLL+YEAE+FC L++K+  +++V RV+
Sbjct: 301 HVTTNPIQRSIVWTMTLPEDIAQSLPLGSKIPYVLLLYEAEDFCNLVAKKELLENVYRVR 360

Query: 361 SRYPSHTICYLTNRLMAFINKREQEKYKNLTCSNDWIRPPVEEELAKLTTHFVKVRSRQC 420
             YPS+T+CYLTN+L++++NK+E+ +YK+      W +PP++E +AKL+TH++ V SR C
Sbjct: 361 DEYPSYTMCYLTNKLLSYVNKKERVEYKDPVNGCGWRKPPIDEAIAKLSTHYIGVHSRHC 420

Query: 421 VDEAEVADHIVGLTCGLASCQFRKKLTHLSVNANGSIIPKDCVDRNMIKKSLWLKALVAI 480
           VDEAEVADH+V LT  LA CQ RKKLT LSV A+G+++ K+  D+++I++S+WLK LVAI
Sbjct: 421 VDEAEVADHVVRLTSSLAHCQVRKKLTRLSVYADGTLMSKNAADKHLIRESIWLKVLVAI 480

Query: 481 PKVQPRFAIAVWKKYPTMKSLLSVYMDPRKSVHEKEFLLKDLTIEGLLGDDRRLGEICSK 540
           PKVQPR+AIAV KKYP++KSLL VYMDP  SVHEKEFLLKDL +E L+G D  +GE CSK
Sbjct: 481 PKVQPRYAIAVSKKYPSLKSLLKVYMDPNISVHEKEFLLKDLKVENLVGRDTSVGEACSK 538

Query: 541 RVYRILMAQNGNMRTDDVEEGADLF 559
           R+YR+LM+ +G ++TDDVE GA  F
Sbjct: 541 RIYRVLMSLDGTIKTDDVENGAASF 538

BLAST of CsGy6G026350 vs. TAIR 10
Match: AT2G21800.2 (essential meiotic endonuclease 1A )

HSP 1 Score: 419.9 bits (1078), Expect = 3.3e-117
Identity = 261/567 (46.03%), Postives = 369/567 (65.08%), Query Frame = 0

Query: 1   MSQPIILSDEEDRYSTPIPIPSKKRRTESDLLPNIKSTVLVLDDDPTPQKSNHNSAAS-- 60
           MS  I++SD ED  + P    ++K RT +D         L LD +P+ QK    SA++  
Sbjct: 1   MSDFILISDGEDEATPPPSKRARKNRTPTD---------LNLDTEPSLQKQPPGSASTPF 60

Query: 61  FVPETPLSPQPSSDVAIVKCTKAGLNLHARDLNSDQKFAGISGLICLESDNESESDSQKK 120
           F+ ETPL    S DV ++K +  G    A     +  F G   +I LESD+E     +  
Sbjct: 61  FLDETPL----SDDVTVLK-SSFGSGTGASS-GRENNFFG-KRVISLESDSEDSPGPESS 120

Query: 121 KCEQSEWIGSDCYEMKNIGASLPFDSAGSGSSLGNDDLQEISGDMKHSTFGRDDDIYQVH 180
           K  + E + +D ++       L F   GS  +  +DD   +      S+  +  D  +V 
Sbjct: 121 K--KYEPVYTDSWKKP---CRLEF---GSSDANSDDDPSWMRRASFQSSLSK--DAIEVD 180

Query: 181 DYPEEDNVGMEQNDNSMKTTGRKA---NAEKVTRKKATKDDKISLMEEKKKKKELEKLQK 240
              E+++ G+E+     +T   K+   +A+ + +KK +KD+K    EEKK +KE EKLQK
Sbjct: 181 SDHEKEDTGVEKMGRKKQTITSKSTSLSADSLPKKKMSKDEKTRAAEEKKLQKEQEKLQK 240

Query: 241 AAQKAEAAEMKKMQKEKQKWEKGK-LALKSIVAEIDAKVVE--LGSIGGHLLTRFAEKGI 300
           AA KAE AE KK+++EKQKW K K  ALK IVA ID KV+E   G I G L++   EK I
Sbjct: 241 AASKAEDAEHKKLEREKQKWAKEKDKALKCIVAWIDNKVLEGSFGVITGLLISGLKEKCI 300

Query: 301 TFRIKSNPIERSILWTMNVPEHISAS-SKGLEISYVLLVYEAENFCELLSKESFMDHVAR 360
           T+ + +NPI+RSI+WTM +PE I+ S   G +I YVLL+YEAE+FC L++K+  +++V R
Sbjct: 301 TYHVTTNPIQRSIVWTMTLPEDIAQSLPLGSKIPYVLLLYEAEDFCNLVAKKELLENVYR 360

Query: 361 VQSRYPSHTICYLTNRLMAFINKREQEKYKNLTCSNDWIRPPVEEELAKLTTHFVKVRSR 420
           V+  YPS+T+CYLTN+L++++NK+E+ +YK+      W +PP++E +AKL+TH++ V SR
Sbjct: 361 VRDEYPSYTMCYLTNKLLSYVNKKERVEYKDPVNGCGWRKPPIDEAIAKLSTHYIGVHSR 420

Query: 421 QCVDEAEVADHIVGLTCGLASCQFRKKLTHLSVNANGSIIPKDCVDRNMIKKSLWLKALV 480
            CVDEAEVADH+V LT  LA CQ RKKLT LSV A+G+++ K+  D+++I++S+WLK LV
Sbjct: 421 HCVDEAEVADHVVRLTSSLAHCQVRKKLTRLSVYADGTLMSKNAADKHLIRESIWLKVLV 480

Query: 481 AIPKVQPRFAIAVWKKYPTMKSLLSVYMDPRKSVHEKEFLLKDLTIEGLLGDDRRLGEIC 540
           AIPKVQPR+AIAV KKYP++KSLL VYMDP  SVHEKEFLLKDL +E L+G D  +GE C
Sbjct: 481 AIPKVQPRYAIAVSKKYPSLKSLLKVYMDPNISVHEKEFLLKDLKVENLVGRDTSVGEAC 540

Query: 541 SKRVYRILMAQNGNMRTDDVEEGADLF 559
           SKR+YR+LM+ +G ++TDDVE GA  F
Sbjct: 541 SKRIYRVLMSLDGTIKTDDVENGAASF 541