CsGy6G025770 (gene) Cucumber (Gy14) v2.1

Overview


Name	CsGy6G025770
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description	Cysteine proteinase inhibitor 5-like
Location	Gy14Chr6: 25343242 .. 25343532 (+)
RNA-Seq Expression	CsGy6G025770
Synteny	CsGy6G025770

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGTTCTCATGTAACAACTGGAGCTTATGGTCCGTGTGAGGATCCAAATAGTTTACACGTGAAAAGAATAGCAGAATGGGCAGTGGAAGAATACAACAAAGGAGACCATTTCCTGAGCCTTGTAAGTATATTGAAATGTGAGTCGCAAGTGGTGAATGGAACCAATTGGCGTCTTAAATTGAAGTGTGTAGATCAAATTAACAATCGTTTGGGAATCTATGTGACTGTTGTGTGGGAGAAATTGGACGGAAGCCTGGTGCTCAGTGACTTCGTTCCTCTCCTCAAATGA

mRNA sequence

ATGTGTTCTCATGTAACAACTGGAGCTTATGGTCCGTGTGAGGATCCAAATAGTTTACACGTGAAAAGAATAGCAGAATGGGCAGTGGAAGAATACAACAAAGGAGACCATTTCCTGAGCCTTGTAAGTATATTGAAATGTGAGTCGCAAGTGGTGAATGGAACCAATTGGCGTCTTAAATTGAAGTGTGTAGATCAAATTAACAATCGTTTGGGAATCTATGTGACTGTTGTGTGGGAGAAATTGGACGGAAGCCTGGTGCTCAGTGACTTCGTTCCTCTCCTCAAATGA

Coding sequence (CDS)

ATGTGTTCTCATGTAACAACTGGAGCTTATGGTCCGTGTGAGGATCCAAATAGTTTACACGTGAAAAGAATAGCAGAATGGGCAGTGGAAGAATACAACAAAGGAGACCATTTCCTGAGCCTTGTAAGTATATTGAAATGTGAGTCGCAAGTGGTGAATGGAACCAATTGGCGTCTTAAATTGAAGTGTGTAGATCAAATTAACAATCGTTTGGGAATCTATGTGACTGTTGTGTGGGAGAAATTGGACGGAAGCCTGGTGCTCAGTGACTTCGTTCCTCTCCTCAAATGA

Protein sequence

MCSHVTTGAYGPCEDPNSLHVKRIAEWAVEEYNKGDHFLSLVSILKCESQVVNGTNWRLKLKCVDQINNRLGIYVTVVWEKLDGSLVLSDFVPLLK*

Homology

[Close]

BLAST of CsGy6G025770 vs. ExPASy Swiss-Prot
Match: P86472 (Cysteine proteinase inhibitor 1 OS=Actinidia chinensis var. chinensis OX=1590841 GN=CYT1 PE=1 SV=2)

HSP 1 Score: 52.8 bits (125), Expect = 2.6e-06
Identity = 30/78 (38.46%), Postives = 46/78 (58.97%), Query Frame = 0


Query: 5   VTTGAYGPCEDPNSLHVKRIAEWAVEEYNK-GDHFLSLVSILKCESQVVNGTNWRLKLKC 64
           V  G + P E+ NS  V+ +A++AV E+NK  +  L   S+++  +QVV GTN+RL +  
Sbjct: 27  VAAGGWRPIENLNSAEVQDVAQFAVSEHNKQANDELQYQSVVRGYTQVVAGTNYRLVIAA 86

Query: 65  VDQINNRLGIYVTVVWEK 82
            D     +G Y  VVW+K
Sbjct: 87  KD--GAVVGNYEAVVWDK 102

[Close]

BLAST of CsGy6G025770 vs. ExPASy Swiss-Prot
Match: Q6TPK4 (Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa OX=3627 PE=1 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 3.4e-06
Identity = 30/78 (38.46%), Postives = 45/78 (57.69%), Query Frame = 0


Query: 5   VTTGAYGPCEDPNSLHVKRIAEWAVEEYNK-GDHFLSLVSILKCESQVVNGTNWRLKLKC 64
           V  G + P E  NS  V+ +A++AV E+NK  +  L   S+++  +QVV GTN+RL +  
Sbjct: 27  VAAGGWRPIESLNSAEVQDVAQFAVSEHNKQANDELQYQSVVRGYTQVVAGTNYRLVIAA 86

Query: 65  VDQINNRLGIYVTVVWEK 82
            D     +G Y  VVW+K
Sbjct: 87  KD--GAVVGNYEAVVWDK 102

[Close]

BLAST of CsGy6G025770 vs. ExPASy Swiss-Prot
Match: Q41916 (Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=CYS5 PE=2 SV=2)

HSP 1 Score: 49.3 bits (116), Expect = 2.9e-05
Identity = 28/78 (35.90%), Postives = 44/78 (56.41%), Query Frame = 0


Query: 8   GAYGPCEDPNSLHVKRIAEWAVEEYNK-GDHFLSLVSILKCESQVVNGTNWRLKLKCVDQ 67
           G + P  +     V  I E+AV EYNK  +  L   +++  E+QVV+GTN+RLK+     
Sbjct: 29  GGWSPISNVTDPQVVEIGEFAVSEYNKRSESGLKFETVVSGETQVVSGTNYRLKVAA--- 88

Query: 68  INNRLGI---YVTVVWEK 82
            N+  G+   Y+ +VW+K
Sbjct: 89  -NDGDGVSKNYLAIVWDK 102

[Close]

BLAST of CsGy6G025770 vs. NCBI nr
Match: KAE8647451.1 (hypothetical protein Csa_002769 [Cucumis sativus])

HSP 1 Score: 189 bits (479), Expect = 2.72e-59
Identity = 87/89 (97.75%), Postives = 88/89 (98.88%), Query Frame = 0


Query: 1  MCSHVTTGAYGPCEDPNSLHVKRIAEWAVEEYNKGDHFLSLVSILKCESQVVNGTNWRLK 60
          MCSHVTTGAYGPCEDPNSLHVKRIAEWAVEEYNKGDHFLSLVSILKCESQVVNGTNWRLK
Sbjct: 1  MCSHVTTGAYGPCEDPNSLHVKRIAEWAVEEYNKGDHFLSLVSILKCESQVVNGTNWRLK 60

Query: 61 LKCVDQINNRLGIYVTVVWEKLDGSLVLS 89
          LKCVDQINNRLGIYVTVVWEKLDGSL +S
Sbjct: 61 LKCVDQINNRLGIYVTVVWEKLDGSLCMS 89

[Close]

BLAST of CsGy6G025770 vs. NCBI nr
Match: KAA0040710.1 (cysteine proteinase inhibitor 5-like [Cucumis melo var. makuwa])

HSP 1 Score: 104 bits (260), Expect = 6.81e-27
Identity = 51/89 (57.30%), Postives = 63/89 (70.79%), Query Frame = 0


Query: 8  GAYGPCEDPNSLHVKRIAEWAVEEYNKGDHFLSLVSILKCESQVVNGTNWRLKLKCVDQI 67
          G Y PC++  S H K++ +WAV E+NK  H L+ VS+LKCESQVV+GTNWRL +KC D+ 
Sbjct: 6  GGYVPCQESQSEHAKKLCQWAVAEHNKQGHHLTFVSLLKCESQVVDGTNWRLVMKCKDE- 65

Query: 68 NNRLGIYVTVVWEK-LDGSLVLSDFVPLL 95
          NN  G Y  VVWEK L+G L L  F PLL
Sbjct: 66 NNYEGNYEAVVWEKILEGFLQLISFFPLL 93

[Close]

BLAST of CsGy6G025770 vs. NCBI nr
Match: NP_001267677.1 (cysteine proteinase inhibitor 5-like [Cucumis sativus] >BAA28867.1 cystein proteinase inhibitor [Cucumis sativus])

HSP 1 Score: 104 bits (260), Expect = 7.21e-27
Identity = 53/76 (69.74%), Postives = 57/76 (75.00%), Query Frame = 0


Query: 8  GAYGPCEDPNSLHVKRIAEWAVEEYNKGD-HFLSLVSILKCESQVVNGTNWRLKLKCVDQ 67
          G Y PC+DPN  HVK IAEWAV EYNK   H L+LVSILKCESQVV G NWRL LKC D+
Sbjct: 7  GGYVPCKDPNDPHVKDIAEWAVAEYNKSQGHHLTLVSILKCESQVVAGVNWRLVLKCKDE 66

Query: 68 INNRLGIYVTVVWEKL 82
           NN  G Y TVVWEK+
Sbjct: 67 -NNGEGNYETVVWEKI 81

[Close]

BLAST of CsGy6G025770 vs. NCBI nr
Match: KAE8647756.1 (hypothetical protein Csa_003034 [Cucumis sativus])

HSP 1 Score: 104 bits (260), Expect = 1.17e-24
Identity = 53/76 (69.74%), Postives = 57/76 (75.00%), Query Frame = 0


Query: 8   GAYGPCEDPNSLHVKRIAEWAVEEYNKGD-HFLSLVSILKCESQVVNGTNWRLKLKCVDQ 67
           G Y PC+DPN  HVK IAEWAV EYNK   H L+LVSILKCESQVV G NWRL LKC D+
Sbjct: 220 GGYVPCKDPNDPHVKDIAEWAVAEYNKSQGHHLTLVSILKCESQVVAGVNWRLVLKCKDE 279

Query: 68  INNRLGIYVTVVWEKL 82
            NN  G Y TVVWEK+
Sbjct: 280 -NNGEGNYETVVWEKI 294

[Close]

BLAST of CsGy6G025770 vs. NCBI nr
Match: KAE8645925.1 (hypothetical protein Csa_021376 [Cucumis sativus])

HSP 1 Score: 96.3 bits (238), Expect = 1.95e-23
Identity = 50/85 (58.82%), Postives = 59/85 (69.41%), Query Frame = 0


Query: 1  MCSHVTTGAYGPCEDPNSLHVKRIAEWAVEEYN-KGDH---FLSLVSILKCESQVVNGTN 60
          M SHVT GAYGPCE+ +  H K +A+WAV EYN K  H   +L L+S+LKCESQVV GTN
Sbjct: 1  MSSHVTLGAYGPCENSDGEHAKEVAQWAVTEYNIKHRHERPYLYLLSVLKCESQVVAGTN 60

Query: 61 WRLKLKCVDQINNRLGIYVTVVWEK 81
          WRL LKC D+ N  +     VVWEK
Sbjct: 61 WRLGLKCKDENNIEVNCEA-VVWEK 84

[Close]

BLAST of CsGy6G025770 vs. ExPASy TrEMBL
Match: A0A0A0KHN6 (Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G482230 PE=4 SV=1)

HSP 1 Score: 205 bits (522), Expect = 3.95e-67
Identity = 96/96 (100.00%), Postives = 96/96 (100.00%), Query Frame = 0


Query: 1  MCSHVTTGAYGPCEDPNSLHVKRIAEWAVEEYNKGDHFLSLVSILKCESQVVNGTNWRLK 60
          MCSHVTTGAYGPCEDPNSLHVKRIAEWAVEEYNKGDHFLSLVSILKCESQVVNGTNWRLK
Sbjct: 1  MCSHVTTGAYGPCEDPNSLHVKRIAEWAVEEYNKGDHFLSLVSILKCESQVVNGTNWRLK 60

Query: 61 LKCVDQINNRLGIYVTVVWEKLDGSLVLSDFVPLLK 96
          LKCVDQINNRLGIYVTVVWEKLDGSLVLSDFVPLLK
Sbjct: 61 LKCVDQINNRLGIYVTVVWEKLDGSLVLSDFVPLLK 96

[Close]

BLAST of CsGy6G025770 vs. ExPASy TrEMBL
Match: A0A5A7TBL3 (Cysteine proteinase inhibitor 5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold529G00310 PE=4 SV=1)

HSP 1 Score: 104 bits (260), Expect = 3.30e-27
Identity = 51/89 (57.30%), Postives = 63/89 (70.79%), Query Frame = 0


Query: 8  GAYGPCEDPNSLHVKRIAEWAVEEYNKGDHFLSLVSILKCESQVVNGTNWRLKLKCVDQI 67
          G Y PC++  S H K++ +WAV E+NK  H L+ VS+LKCESQVV+GTNWRL +KC D+ 
Sbjct: 6  GGYVPCQESQSEHAKKLCQWAVAEHNKQGHHLTFVSLLKCESQVVDGTNWRLVMKCKDE- 65

Query: 68 NNRLGIYVTVVWEK-LDGSLVLSDFVPLL 95
          NN  G Y  VVWEK L+G L L  F PLL
Sbjct: 66 NNYEGNYEAVVWEKILEGFLQLISFFPLL 93

[Close]

BLAST of CsGy6G025770 vs. ExPASy TrEMBL
Match: O80389 (Cystein proteinase inhibitor OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 104 bits (260), Expect = 3.49e-27
Identity = 53/76 (69.74%), Postives = 57/76 (75.00%), Query Frame = 0


Query: 8  GAYGPCEDPNSLHVKRIAEWAVEEYNKGD-HFLSLVSILKCESQVVNGTNWRLKLKCVDQ 67
          G Y PC+DPN  HVK IAEWAV EYNK   H L+LVSILKCESQVV G NWRL LKC D+
Sbjct: 7  GGYVPCKDPNDPHVKDIAEWAVAEYNKSQGHHLTLVSILKCESQVVAGVNWRLVLKCKDE 66

Query: 68 INNRLGIYVTVVWEKL 82
           NN  G Y TVVWEK+
Sbjct: 67 -NNGEGNYETVVWEKI 81

[Close]

BLAST of CsGy6G025770 vs. ExPASy TrEMBL
Match: A0A0A0KHK7 (Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G459990 PE=4 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 9.44e-24
Identity = 50/85 (58.82%), Postives = 59/85 (69.41%), Query Frame = 0


Query: 1  MCSHVTTGAYGPCEDPNSLHVKRIAEWAVEEYN-KGDH---FLSLVSILKCESQVVNGTN 60
          M SHVT GAYGPCE+ +  H K +A+WAV EYN K  H   +L L+S+LKCESQVV GTN
Sbjct: 1  MSSHVTLGAYGPCENSDGEHAKEVAQWAVTEYNIKHRHERPYLYLLSVLKCESQVVAGTN 60

Query: 61 WRLKLKCVDQINNRLGIYVTVVWEK 81
          WRL LKC D+ N  +     VVWEK
Sbjct: 61 WRLGLKCKDENNIEVNCEA-VVWEK 84

[Close]

BLAST of CsGy6G025770 vs. ExPASy TrEMBL
Match: A0A0A0KF17 (Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G460000 PE=4 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 5.12e-21
Identity = 50/97 (51.55%), Postives = 61/97 (62.89%), Query Frame = 0


Query: 1  MCSHVTTGAYGPCEDPNSLHVKRIAEWAVEEYN-KGDH---FLSLVSILKCESQVVNGTN 60
          M S    G YGPCE+ +  H K +A+WAV EYN K  H   +L L+S+LKCESQVV GTN
Sbjct: 1  MSSDSIKGGYGPCENSDGEHAKEVAQWAVTEYNIKHRHERPYLYLLSVLKCESQVVAGTN 60

Query: 61 WRLKLKCVDQINNRLGIYVTVVWEKL-DGSLVLSDFV 92
          WRL LKC D+ N  +     VVWEK  +  L L+ FV
Sbjct: 61 WRLGLKCKDENNIEVNCEA-VVWEKRWENFLELTSFV 96

[Close]

BLAST of CsGy6G025770 vs. TAIR 10
Match: AT5G47550.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 49.3 bits (116), Expect = 2.0e-06
Identity = 28/78 (35.90%), Postives = 44/78 (56.41%), Query Frame = 0


Query: 8   GAYGPCEDPNSLHVKRIAEWAVEEYNK-GDHFLSLVSILKCESQVVNGTNWRLKLKCVDQ 67
           G + P  +     V  I E+AV EYNK  +  L   +++  E+QVV+GTN+RLK+     
Sbjct: 29  GGWSPISNVTDPQVVEIGEFAVSEYNKRSESGLKFETVVSGETQVVSGTNYRLKVAA--- 88

Query: 68  INNRLGI---YVTVVWEK 82
            N+  G+   Y+ +VW+K
Sbjct: 89  -NDGDGVSKNYLAIVWDK 102

The following BLAST results are available for this feature:

BLAST of CsGy6G025770 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P86472	2.6e-06	38.46	Cysteine proteinase inhibitor 1 OS=Actinidia chinensis var. chinensis OX=1590841...	[more]
Q6TPK4	3.4e-06	38.46	Cysteine proteinase inhibitor 1 OS=Actinidia deliciosa OX=3627 PE=1 SV=1	[more]
Q41916	2.9e-05	35.90	Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=CYS5 PE=2 SV=...	[more]

BLAST of CsGy6G025770 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8647451.1	2.72e-59	97.75	hypothetical protein Csa_002769 [Cucumis sativus]	[more]
KAA0040710.1	6.81e-27	57.30	cysteine proteinase inhibitor 5-like [Cucumis melo var. makuwa]	[more]
NP_001267677.1	7.21e-27	69.74	cysteine proteinase inhibitor 5-like [Cucumis sativus] >BAA28867.1 cystein prote...	[more]
KAE8647756.1	1.17e-24	69.74	hypothetical protein Csa_003034 [Cucumis sativus]	[more]
KAE8645925.1	1.95e-23	58.82	hypothetical protein Csa_021376 [Cucumis sativus]	[more]

BLAST of CsGy6G025770 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KHN6	3.95e-67	100.00	Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G482230 PE...	[more]
A0A5A7TBL3	3.30e-27	57.30	Cysteine proteinase inhibitor 5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
O80389	3.49e-27	69.74	Cystein proteinase inhibitor OS=Cucumis sativus OX=3659 PE=2 SV=1	[more]
A0A0A0KHK7	9.44e-24	58.82	Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G459990 PE...	[more]
A0A0A0KF17	5.12e-21	51.55	Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G460000 PE...	[more]

BLAST of CsGy6G025770 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G47550.1	2.0e-06	35.90	Cystatin/monellin superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.10.450.10		coord: 4..95 e-value: 2.3E-16 score: 61.7
None	No IPR available	PANTHER	PTHR47364:SF2	CYSTEINE PROTEINASE INHIBITOR 5	coord: 4..81
None	No IPR available	PANTHER	PTHR47364	CYSTEINE PROTEINASE INHIBITOR 5	coord: 4..81
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 4..83
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 17..87 e-value: 5.4E-14 score: 52.3
IPR000010	Cystatin domain	CDD	cd00042	CY	coord: 15..65 e-value: 3.13945E-7 score: 42.8464

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsGy6G025770.1	CsGy6G025770.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010466	negative regulation of peptidase activity
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity