CsGy6G025670 (gene) Cucumber (Gy14) v2.1

Overview


Name	CsGy6G025670
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description	Aspartic acid proteinase inhibitor
Location	Gy14Chr6: 25192351 .. 25193034 (+)
RNA-Seq Expression	CsGy6G025670
Synteny	CsGy6G025670

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CACAAACCATATTACTATCTTTCTTCAAGTGGCCGGTTTACTTTTTTTTTTTAAAGAGAAATCAAGGTCATGGCTGCCGCTGGAATTGGACAACCTTCGATCGTGATTGGTACCTGGAATGCGATACCGAATGACGATCCAAAAGTAATAGAGATGGCAGAGTATGCAGTGAAAGAACAGGGAGAAGAATTGATCCTGAACAGTGTACAAGCTGGACAGATCGTTGAGGTGTTTTTGAGTGGCGACACTTATTACAACCTTCTCATCCTTGCTCATCATCCTCACACATTTCAGGGGTTTTATAGCACCATTGTGATGGAAAATACATCCAAAAATGTGAAGCAGGTCGTATCTTTTGTTCCTCTTCGTTAATGATGCAATGATGATGGATGCATCAATGATCTACAATCTCAACAAATTATCTACTCTCTCTCACTTGTTTATGTGTATGCATGTTATACATGCAATTGGAATGAAAAGTAGTACAAATTTAGATAAAATAAAAGTATAAAAAGCAGGTATGCTTTTGGTGTGTTTTCATCTAAATTCTCTCTCGTTTTAATTTATCTAGACCCAAAATTGAGACAGATTCAAAATCTAGAATTACGCCCACATGATGACTTCAACATTTTCTTGCACGATGAGTCCTTTGTATCAATTAGAACAAATTATGATAATTCTATT

mRNA sequence

CACAAACCATATTACTATCTTTCTTCAAGTGGCCGGTTTACTTTTTTTTTTTAAAGAGAAATCAAGGTCATGGCTGCCGCTGGAATTGGACAACCTTCGATCGTGATTGGTACCTGGAATGCGATACCGAATGACGATCCAAAAGTAATAGAGATGGCAGAGTATGCAGTGAAAGAACAGGGAGAAGAATTGATCCTGAACAGTGTACAAGCTGGACAGATCGTTGAGGTGTTTTTGAGTGGCGACACTTATTACAACCTTCTCATCCTTGCTCATCATCCTCACACATTTCAGGGGTTTTATAGCACCATTGTGATGGAAAATACATCCAAAAATGTGAAGCAGGTCGTATCTTTTGTTCCTCTTCGTTAATGATGCAATGATGATGGATGCATCAATGATCTACAATCTCAACAAATTATCTACTCTCTCTCACTTGTTTATGTGTATGCATGTTATACATGCAATTGGAATGAAAAGTAGTACAAATTTAGATAAAATAAAAGTATAAAAAGCAGGTATGCTTTTGGTGTGTTTTCATCTAAATTCTCTCTCGTTTTAATTTATCTAGACCCAAAATTGAGACAGATTCAAAATCTAGAATTACGCCCACATGATGACTTCAACATTTTCTTGCACGATGAGTCCTTTGTATCAATTAGAACAAATTATGATAATTCTATT

Coding sequence (CDS)

ATGGCTGCCGCTGGAATTGGACAACCTTCGATCGTGATTGGTACCTGGAATGCGATACCGAATGACGATCCAAAAGTAATAGAGATGGCAGAGTATGCAGTGAAAGAACAGGGAGAAGAATTGATCCTGAACAGTGTACAAGCTGGACAGATCGTTGAGGTGTTTTTGAGTGGCGACACTTATTACAACCTTCTCATCCTTGCTCATCATCCTCACACATTTCAGGGGTTTTATAGCACCATTGTGATGGAAAATACATCCAAAAATGTGAAGCAGGTCGTATCTTTTGTTCCTCTTCGTTAA

Protein sequence

MAAAGIGQPSIVIGTWNAIPNDDPKVIEMAEYAVKEQGEELILNSVQAGQIVEVFLSGDTYYNLLILAHHPHTFQGFYSTIVMENTSKNVKQVVSFVPLR*

Homology

[Close]

BLAST of CsGy6G025670 vs. NCBI nr
Match: KAE8645938.1 (hypothetical protein Csa_021378 [Cucumis sativus])

HSP 1 Score: 201 bits (510), Expect = 7.42e-65
Identity = 100/100 (100.00%), Postives = 100/100 (100.00%), Query Frame = 0


Query: 1   MAAAGIGQPSIVIGTWNAIPNDDPKVIEMAEYAVKEQGEELILNSVQAGQIVEVFLSGDT 60
           MAAAGIGQPSIVIGTWNAIPNDDPKVIEMAEYAVKEQGEELILNSVQAGQIVEVFLSGDT
Sbjct: 1   MAAAGIGQPSIVIGTWNAIPNDDPKVIEMAEYAVKEQGEELILNSVQAGQIVEVFLSGDT 60

Query: 61  YYNLLILAHHPHTFQGFYSTIVMENTSKNVKQVVSFVPLR 100
           YYNLLILAHHPHTFQGFYSTIVMENTSKNVKQVVSFVPLR
Sbjct: 61  YYNLLILAHHPHTFQGFYSTIVMENTSKNVKQVVSFVPLR 100

[Close]

BLAST of CsGy6G025670 vs. NCBI nr
Match: KAE8647440.1 (hypothetical protein Csa_003293, partial [Cucumis sativus])

HSP 1 Score: 149 bits (375), Expect = 3.07e-44
Identity = 75/75 (100.00%), Postives = 75/75 (100.00%), Query Frame = 0


Query: 26  VIEMAEYAVKEQGEELILNSVQAGQIVEVFLSGDTYYNLLILAHHPHTFQGFYSTIVMEN 85
           VIEMAEYAVKEQGEELILNSVQAGQIVEVFLSGDTYYNLLILAHHPHTFQGFYSTIVMEN
Sbjct: 28  VIEMAEYAVKEQGEELILNSVQAGQIVEVFLSGDTYYNLLILAHHPHTFQGFYSTIVMEN 87

Query: 86  TSKNVKQVVSFVPLR 100
           TSKNVKQVVSFVPLR
Sbjct: 88  TSKNVKQVVSFVPLR 102

[Close]

BLAST of CsGy6G025670 vs. NCBI nr
Match: KAA0025192.1 (aspartic protease inhibitor [Cucumis melo var. makuwa] >TYK07473.1 aspartic protease inhibitor [Cucumis melo var. makuwa])

HSP 1 Score: 145 bits (365), Expect = 8.57e-43
Identity = 71/90 (78.89%), Postives = 81/90 (90.00%), Query Frame = 0


Query: 10 SIVIGTWNAIPNDDPKVIEMAEYAVKEQGEELILNSVQAGQIVEVFLSGDTYYNLLILAH 69
          SIVIGT N+I  DDPKVIEMAEYA++EQ ++LILNSV  GQIV+VFLSG+TYYNL+ILAH
Sbjct: 6  SIVIGTSNSITKDDPKVIEMAEYAIREQNQDLILNSVADGQIVKVFLSGNTYYNLVILAH 65

Query: 70 HPHTFQGFYSTIVMENTSKNVKQVVSFVPL 99
          HPHTFQGFY+  V+ENTSKNVK VVSFVPL
Sbjct: 66 HPHTFQGFYNATVLENTSKNVKVVVSFVPL 95

[Close]

BLAST of CsGy6G025670 vs. NCBI nr
Match: XP_004151410.1 (uncharacterized protein LOC101213950 [Cucumis sativus] >XP_031744693.1 uncharacterized protein LOC116405126 [Cucumis sativus] >KAE8645937.1 hypothetical protein Csa_021388 [Cucumis sativus])

HSP 1 Score: 143 bits (360), Expect = 5.59e-42
Identity = 78/100 (78.00%), Postives = 83/100 (83.00%), Query Frame = 0


Query: 1  MAAAGIGQPSIVIGTWNAIPNDDPKVIEMAEYAVKEQGEE-LILNSVQAGQIVEVFLSGD 60
          M  AG G PSIVIG+ N I  DDPKVIEMAEYAVKEQGE+ LILNSV  GQIV VFL G+
Sbjct: 1  MDTAGFG-PSIVIGSCNPILKDDPKVIEMAEYAVKEQGEDHLILNSVADGQIVTVFLGGN 60

Query: 61 TYYNLLILAHHPHTFQGFYSTIVMENTSKNVKQVVSFVPL 99
          TYYNLLILAHHP TFQGFYS IV+ENTSKNVK+VVSF  L
Sbjct: 61 TYYNLLILAHHPRTFQGFYSAIVLENTSKNVKEVVSFFRL 99

[Close]

BLAST of CsGy6G025670 vs. NCBI nr
Match: KAE8647441.1 (hypothetical protein Csa_004388, partial [Cucumis sativus])

HSP 1 Score: 135 bits (341), Expect = 3.46e-39
Identity = 73/93 (78.49%), Postives = 78/93 (83.87%), Query Frame = 0


Query: 1  MAAAGIGQPSIVIGTWNAIPNDDPKVIEMAEYAVKEQGEE-LILNSVQAGQIVEVFLSGD 60
          M  AG G PSIVIG+ N I  DDPKVIEMAEYAVKEQGE+ LILNSV  GQIV VFL G+
Sbjct: 1  MDTAGFG-PSIVIGSCNPILKDDPKVIEMAEYAVKEQGEDHLILNSVADGQIVTVFLGGN 60

Query: 61 TYYNLLILAHHPHTFQGFYSTIVMENTSKNVKQ 92
          TYYNLLILAHHP TFQGFYS IV+ENTSKNVK+
Sbjct: 61 TYYNLLILAHHPRTFQGFYSAIVLENTSKNVKE 92

[Close]

BLAST of CsGy6G025670 vs. ExPASy TrEMBL
Match: A0A5A7SJ25 (Aspartic protease inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G002060 PE=4 SV=1)

HSP 1 Score: 145 bits (365), Expect = 4.15e-43
Identity = 71/90 (78.89%), Postives = 81/90 (90.00%), Query Frame = 0


Query: 10 SIVIGTWNAIPNDDPKVIEMAEYAVKEQGEELILNSVQAGQIVEVFLSGDTYYNLLILAH 69
          SIVIGT N+I  DDPKVIEMAEYA++EQ ++LILNSV  GQIV+VFLSG+TYYNL+ILAH
Sbjct: 6  SIVIGTSNSITKDDPKVIEMAEYAIREQNQDLILNSVADGQIVKVFLSGNTYYNLVILAH 65

Query: 70 HPHTFQGFYSTIVMENTSKNVKQVVSFVPL 99
          HPHTFQGFY+  V+ENTSKNVK VVSFVPL
Sbjct: 66 HPHTFQGFYNATVLENTSKNVKVVVSFVPL 95

[Close]

BLAST of CsGy6G025670 vs. ExPASy TrEMBL
Match: Q2V8I8 (Aspartic acid proteinase inhibitor (Fragment) OS=Cucurbita maxima OX=3661 GN=API PE=4 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 8.25e-16
Identity = 42/93 (45.16%), Postives = 59/93 (63.44%), Query Frame = 0


Query: 9   PSIVIGTWNAIPNDDPKVIEMAEYAVKEQGEE-LILNSVQAGQIVEVFLSGDTYYNLLIL 68
           P   IG    I  +DP+V E+AE+A+K+  E+ LIL  V AGQI++     D YYNL++ 
Sbjct: 9   PGPAIGEVIGISVNDPRVKEIAEFALKQHAEQNLILAGVDAGQIIKGIPHWDNYYNLILS 68

Query: 69  AHH-PHTFQGFYSTIVMENTSKNVKQVVSFVPL 99
           A H PH F  FY+ +V+E  S N  +++SFVPL
Sbjct: 69  AKHSPHEFSKFYNVVVLEKASDNSLKLISFVPL 101

[Close]

BLAST of CsGy6G025670 vs. ExPASy TrEMBL
Match: O81131 (Aspartic proteinase inhibitor (Fragment) OS=Cucurbita maxima OX=3661 GN=API-1 PE=2 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 1.34e-15
Identity = 42/93 (45.16%), Postives = 59/93 (63.44%), Query Frame = 0


Query: 9  PSIVIGTWNAIPNDDPKVIEMAEYAVKEQGEE-LILNSVQAGQIVEVFLSGDTYYNLLIL 68
          P   IG    I  +DP+V E+AE+A+K+  E+ LIL  V AGQI++     D YYNL++ 
Sbjct: 1  PGPAIGEVIGISVNDPRVKEIAEFALKQHAEQNLILAGVDAGQIIKGIPHWDNYYNLILS 60

Query: 69 AHH-PHTFQGFYSTIVMENTSKNVKQVVSFVPL 99
          A H PH F  FY+ +V+E  S N  ++V+FVPL
Sbjct: 61 AKHSPHEFSKFYNVVVLEKASDNSLKLVAFVPL 93

[Close]

BLAST of CsGy6G025670 vs. ExPASy TrEMBL
Match: Q6DLC9 (Aspartic protease inhibitor OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 1.66e-15
Identity = 42/93 (45.16%), Postives = 59/93 (63.44%), Query Frame = 0


Query: 9   PSIVIGTWNAIPNDDPKVIEMAEYAVKEQGEE-LILNSVQAGQIVEVFLSGDTYYNLLIL 68
           P   IG    I  +DP+V E+AE+A+K+  E+ LIL  V AGQI++     D YYNL++ 
Sbjct: 9   PGPAIGEVIGISVNDPRVKEIAEFALKQHAEQNLILAGVDAGQIIKGIPHWDNYYNLILS 68

Query: 69  AHH-PHTFQGFYSTIVMENTSKNVKQVVSFVPL 99
           A H PH F  FY+ +V+E  S N  ++V+FVPL
Sbjct: 69  AKHSPHEFSKFYNVVVLEKASDNSLKLVAFVPL 101

[Close]

BLAST of CsGy6G025670 vs. ExPASy TrEMBL
Match: O81132 (Aspartic proteinase inhibitor (Fragment) OS=Cucurbita maxima OX=3661 GN=API-2 PE=2 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 2.70e-15
Identity = 41/93 (44.09%), Postives = 59/93 (63.44%), Query Frame = 0


Query: 9  PSIVIGTWNAIPNDDPKVIEMAEYAVKEQGEE-LILNSVQAGQIVEVFLSGDTYYNLLIL 68
          P   IG    I  +DP+V E+AE+A+K+  E+ LIL  V AGQI++     + YYNL++ 
Sbjct: 1  PGPAIGEVIGISVNDPRVKEIAEFALKQHAEQNLILAGVDAGQIIKGIPDWNNYYNLILS 60

Query: 69 AHH-PHTFQGFYSTIVMENTSKNVKQVVSFVPL 99
          A H PH F  FY+ +V+E  S N  +++SFVPL
Sbjct: 61 AKHSPHEFSKFYNVVVLEKASDNSLKLISFVPL 93

The following BLAST results are available for this feature:

BLAST of CsGy6G025670 vs.


Match Name	E-value	Identity	Description

BLAST of CsGy6G025670 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8645938.1	7.42e-65	100.00	hypothetical protein Csa_021378 [Cucumis sativus]	[more]
KAE8647440.1	3.07e-44	100.00	hypothetical protein Csa_003293, partial [Cucumis sativus]	[more]
KAA0025192.1	8.57e-43	78.89	aspartic protease inhibitor [Cucumis melo var. makuwa] >TYK07473.1 aspartic prot...	[more]
XP_004151410.1	5.59e-42	78.00	uncharacterized protein LOC101213950 [Cucumis sativus] >XP_031744693.1 uncharact...	[more]
KAE8647441.1	3.46e-39	78.49	hypothetical protein Csa_004388, partial [Cucumis sativus]	[more]

BLAST of CsGy6G025670 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7SJ25	4.15e-43	78.89	Aspartic protease inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
Q2V8I8	8.25e-16	45.16	Aspartic acid proteinase inhibitor (Fragment) OS=Cucurbita maxima OX=3661 GN=API...	[more]
O81131	1.34e-15	45.16	Aspartic proteinase inhibitor (Fragment) OS=Cucurbita maxima OX=3661 GN=API-1 PE...	[more]
Q6DLC9	1.66e-15	45.16	Aspartic protease inhibitor OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]
O81132	2.70e-15	44.09	Aspartic proteinase inhibitor (Fragment) OS=Cucurbita maxima OX=3661 GN=API-2 PE...	[more]

BLAST of CsGy6G025670 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 22..97 e-value: 2.4E-8 score: 34.2
None	No IPR available	GENE3D	3.10.450.10		coord: 8..99 e-value: 2.1E-17 score: 65.1
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 14..99

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsGy6G025670.2	CsGy6G025670.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity