CsGy6G022100 (gene) Cucumber (Gy14) v2.1

Overview


Name	CsGy6G022100
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description	taxadiene 5-alpha hydroxylase
Location	Gy14Chr6: 22664168 .. 22666083 (+)
RNA-Seq Expression	CsGy6G022100
Synteny	CsGy6G022100

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTCTCTTTTTTAGCTGCAATACTGTTAATAATATCGATTGCTTTTGTGTTGAGAAGCACTAAGAGAAGCAGAAATGTCCCACCAGGGGCAATGGGTCTACCTTGGATTGGAGAAACAATGGTTTTTTACAGAGCTCAAAGTAAAAACAGATTGTTTGAAGACTTTGTTGAGCCAAGAGTTGCAAAATATGGAAAAATCTTCAAAACAAACTTAATGGGATCTCCAACAGTGGTTGTTAATGGAGCTGAAGCCAATAGATTTTTCTTGTCAAATGAGTTTAAGTTAGTGGTAAGTTCATGGCCATCGAGCTCTGTTCAGTTGATGGGTGAAGAATGTATTATGCAGAAGGAAGGTGAAAAGCATAGATGTGTTCGTGGTCTTATAGCTGCAACTCTTTCTACTTCGAGTCTTGACGTTATGATGCCTAAACTGTGCAATACAATTCAAGTTTATTTGGATACTAAATGGGTTGGTTGTGACACAATTGGTCTCTTTCACTCGGCTAAAGTATTGACATTTACAATTGTGTTTGAGTGTTTATTGGGTATTCAAGTGGAGCCACGTGTGTTGACGATGTTCGAGAGGGTTTTGGAAGGTGTTTTTGCACCACCATTCAGGTAGTTCAAAGTTATAAGCTGATTGGTTATGTTCAAGTTAATTGCATTATCATTTTCCTTTTGCACCACCAAGTTTTTATAAACTTAATTTTCAACGATAAAATAGTTATCAAACGAGACTTATCTCCTTTAACTTTTAAAACTTTGATTGATTTGTAAATACATTGGTGGAACTTGAATAACCTGGTAAAAATAGTTATTATAAGTTCTATTTCAAAAAATGAAAAACTAAAAGAAAATTGTTATCACATATTCTTAACACAGGTTTCCAGGAACAAGATTTTCAAGAGCCAAGAAAGCAAGACTGGAGATAGAGACAACGCTAAGGGAAGTGGTGAGAGAAAAGAGGAGAAAAATAGAAGGAAAGCTAGAAGAAGAAGAATGTGGGTCATTGCTTTCAAGGATGGTGAGAGCAATGATAGGAGGGGAAATCAGTGAAATGGAAGTCATAGACAATATTGTTTTGCTTGTTTTTGCAGCTCATGATACAACCTCCTTTGCCATTGCCATGACTTTCAAAATGTTGTCTCAGCACACAAATTGCTACACACTTCTTCTCCAGGGTAATCTTTCCTTTTTTTCAATCTTTTGAAGTAATACTCTAAGATCATAATAAACCAAAAATGGCTATTCTGATGGTTGACAAAATATCATTTTGCTGATTTAAACGGGTTCTAAAATGTTTTACTTTTAAAACAGAACACACTGACATAATAAGAAGCAAGGGGCCAGGCCAGAATTTGACAATGGAAGACATAAAGAAGATGAAATATACTTGGCAGGTAGCTCGTGAGAGCATTAGGTTGTTTCCCCCTATATTTGGTTCTTTCAGGAAAGCCATTTCAGACATTGAATATGAGAGCTTTCTCATTCCCAAAGGATGGAAGGTGAAAATTTCAATCCTTACATCTGCAGTCTAATTCTTATCCAGAAATGACCCTGATTTTACCTCGAATCATTTTGGTTTCATGGCAGGTCTTGTGGACGACGTACGGAACACATTACGGAGAAGAGTACTTTGAGGATCCATTGAGTTTCAAGCCAAGCAGATTTGAGGAACCTGTTTCACAGTATGTTTATGTTCCATTTGGAGGAGGGCCAAGAGCTTGTGCAGGCTACCAACTAGCGAAATTGAACATACTCATTTTTGTACACTCGGTTGTAACTCGTTATGATTGGAGTTTAGTTCATCCAGATGAGCCAATCATAATGGATCCTCTCCCTGTCCCTTATCATGGAATGCCTATTAAAATTTCCCCAAAATCACAAGTTTGTTCCAACGTTCACCAAATGTAA

mRNA sequence

ATGCTCTCTTTTTTAGCTGCAATACTGTTAATAATATCGATTGCTTTTGTGTTGAGAAGCACTAAGAGAAGCAGAAATGTCCCACCAGGGGCAATGGGTCTACCTTGGATTGGAGAAACAATGGTTTTTTACAGAGCTCAAAGTAAAAACAGATTGTTTGAAGACTTTGTTGAGCCAAGAGTTGCAAAATATGGAAAAATCTTCAAAACAAACTTAATGGGATCTCCAACAGTGGTTGTTAATGGAGCTGAAGCCAATAGATTTTTCTTGTCAAATGAGTTTAAGTTAGTGGTAAGTTCATGGCCATCGAGCTCTGTTCAGTTGATGGGTGAAGAATGTATTATGCAGAAGGAAGGTGAAAAGCATAGATGTGTTCGTGGTCTTATAGCTGCAACTCTTTCTACTTCGAGTCTTGACGTTATGATGCCTAAACTGTGCAATACAATTCAAGTTTATTTGGATACTAAATGGGTTGGTTGTGACACAATTGGTCTCTTTCACTCGGCTAAAGTATTGACATTTACAATTGTGTTTGAGTGTTTATTGGGTATTCAAGTGGAGCCACGTGTGTTGACGATGTTCGAGAGGGTTTTGGAAGGTGTTTTTGCACCACCATTCAGGTTTCCAGGAACAAGATTTTCAAGAGCCAAGAAAGCAAGACTGGAGATAGAGACAACGCTAAGGGAAGTGGTGAGAGAAAAGAGGAGAAAAATAGAAGGAAAGCTAGAAGAAGAAGAATGTGGGTCATTGCTTTCAAGGATGGTGAGAGCAATGATAGGAGGGGAAATCAGTGAAATGGAAGTCATAGACAATATTGTTTTGCTTGTTTTTGCAGCTCATGATACAACCTCCTTTGCCATTGCCATGACTTTCAAAATGTTGTCTCAGCACACAAATTGCTACACACTTCTTCTCCAGGAACACACTGACATAATAAGAAGCAAGGGGCCAGGCCAGAATTTGACAATGGAAGACATAAAGAAGATGAAATATACTTGGCAGGTAGCTCGTGAGAGCATTAGGTTGTTTCCCCCTATATTTGGTTCTTTCAGGAAAGCCATTTCAGACATTGAATATGAGAGCTTTCTCATTCCCAAAGGATGGAAGGTCTTGTGGACGACGTACGGAACACATTACGGAGAAGAGTACTTTGAGGATCCATTGAGTTTCAAGCCAAGCAGATTTGAGGAACCTGTTTCACAGTATGTTTATGTTCCATTTGGAGGAGGGCCAAGAGCTTGTGCAGGCTACCAACTAGCGAAATTGAACATACTCATTTTTGTACACTCGGTTGTAACTCGTTATGATTGGAGTTTAGTTCATCCAGATGAGCCAATCATAATGGATCCTCTCCCTGTCCCTTATCATGGAATGCCTATTAAAATTTCCCCAAAATCACAAGTTTGTTCCAACGTTCACCAAATGTAA

Coding sequence (CDS)

ATGCTCTCTTTTTTAGCTGCAATACTGTTAATAATATCGATTGCTTTTGTGTTGAGAAGCACTAAGAGAAGCAGAAATGTCCCACCAGGGGCAATGGGTCTACCTTGGATTGGAGAAACAATGGTTTTTTACAGAGCTCAAAGTAAAAACAGATTGTTTGAAGACTTTGTTGAGCCAAGAGTTGCAAAATATGGAAAAATCTTCAAAACAAACTTAATGGGATCTCCAACAGTGGTTGTTAATGGAGCTGAAGCCAATAGATTTTTCTTGTCAAATGAGTTTAAGTTAGTGGTAAGTTCATGGCCATCGAGCTCTGTTCAGTTGATGGGTGAAGAATGTATTATGCAGAAGGAAGGTGAAAAGCATAGATGTGTTCGTGGTCTTATAGCTGCAACTCTTTCTACTTCGAGTCTTGACGTTATGATGCCTAAACTGTGCAATACAATTCAAGTTTATTTGGATACTAAATGGGTTGGTTGTGACACAATTGGTCTCTTTCACTCGGCTAAAGTATTGACATTTACAATTGTGTTTGAGTGTTTATTGGGTATTCAAGTGGAGCCACGTGTGTTGACGATGTTCGAGAGGGTTTTGGAAGGTGTTTTTGCACCACCATTCAGGTTTCCAGGAACAAGATTTTCAAGAGCCAAGAAAGCAAGACTGGAGATAGAGACAACGCTAAGGGAAGTGGTGAGAGAAAAGAGGAGAAAAATAGAAGGAAAGCTAGAAGAAGAAGAATGTGGGTCATTGCTTTCAAGGATGGTGAGAGCAATGATAGGAGGGGAAATCAGTGAAATGGAAGTCATAGACAATATTGTTTTGCTTGTTTTTGCAGCTCATGATACAACCTCCTTTGCCATTGCCATGACTTTCAAAATGTTGTCTCAGCACACAAATTGCTACACACTTCTTCTCCAGGAACACACTGACATAATAAGAAGCAAGGGGCCAGGCCAGAATTTGACAATGGAAGACATAAAGAAGATGAAATATACTTGGCAGGTAGCTCGTGAGAGCATTAGGTTGTTTCCCCCTATATTTGGTTCTTTCAGGAAAGCCATTTCAGACATTGAATATGAGAGCTTTCTCATTCCCAAAGGATGGAAGGTCTTGTGGACGACGTACGGAACACATTACGGAGAAGAGTACTTTGAGGATCCATTGAGTTTCAAGCCAAGCAGATTTGAGGAACCTGTTTCACAGTATGTTTATGTTCCATTTGGAGGAGGGCCAAGAGCTTGTGCAGGCTACCAACTAGCGAAATTGAACATACTCATTTTTGTACACTCGGTTGTAACTCGTTATGATTGGAGTTTAGTTCATCCAGATGAGCCAATCATAATGGATCCTCTCCCTGTCCCTTATCATGGAATGCCTATTAAAATTTCCCCAAAATCACAAGTTTGTTCCAACGTTCACCAAATGTAA

Protein sequence

MLSFLAAILLIISIAFVLRSTKRSRNVPPGAMGLPWIGETMVFYRAQSKNRLFEDFVEPRVAKYGKIFKTNLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIAATLSTSSLDVMMPKLCNTIQVYLDTKWVGCDTIGLFHSAKVLTFTIVFECLLGIQVEPRVLTMFERVLEGVFAPPFRFPGTRFSRAKKARLEIETTLREVVREKRRKIEGKLEEEECGSLLSRMVRAMIGGEISEMEVIDNIVLLVFAAHDTTSFAIAMTFKMLSQHTNCYTLLLQEHTDIIRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWKVLWTTYGTHYGEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFVHSVVTRYDWSLVHPDEPIIMDPLPVPYHGMPIKISPKSQVCSNVHQM*

Homology

[Close]

BLAST of CsGy6G022100 vs. ExPASy Swiss-Prot
Match: Q6WG30 (Taxadiene 5-alpha hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=2)

HSP 1 Score: 348.2 bits (892), Expect = 1.5e-94
Identity = 188/475 (39.58%), Postives = 292/475 (61.47%), Query Frame = 0


Query: 2   LSFLAAILLIISIAFVLRSTKRSR-NVPPGAMGLPWIGETMVFYRAQSKNRLFEDFVEPR 61
           LS +A ILL++    + RS + S   +PPG +G+P+IGE+ +F RA   N L E F + R
Sbjct: 29  LSAIAGILLLL---LLFRSKRHSSLKLPPGKLGIPFIGESFIFLRALRSNSL-EQFFDER 88

Query: 62  VAKYGKIFKTNLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSSSVQLMGEECIMQKEGE 121
           V K+G +FKT+L+G PTVV+ G   NR  LSNE KLV  SWP+  ++LMGE  +  + GE
Sbjct: 89  VKKFGLVFKTSLIGHPTVVLCGPAGNRLILSNEEKLVQMSWPAQFMKLMGENSVATRRGE 148

Query: 122 KHRCVRGLIAATLSTSSLDVMMPKLCNTIQVYLDTKWVGCDTIGLFHSAKVLTF---TIV 181
            H  +R  +A      +L   + K+   IQ +++ KW G D + +    + L F    I+
Sbjct: 149 DHIVMRSALAGFFGPGALQSYIGKMNTEIQSHINEKWKGKDEVNVLPLVRELVFNISAIL 208

Query: 182 FECLLGIQVEPRVLTMFERVLEGVFAPPFRFPGTRFSRAKKARLEIETTLREVVREKRRK 241
           F  +   Q + R+  + E +L G FA P   PG  F RA + R ++   +  ++++++  
Sbjct: 209 FFNIYDKQEQDRLHKLLETILVGSFALPIDLPGFGFHRALQGRAKLNKIMLSLIKKRKED 268

Query: 242 IEGKLEEEECGSLLSRMV--RAMIGGEISEMEVIDNIVLLVFAAHDTTSFAIAMTFKMLS 301
           ++          LLS ++  R   G  ++  E++DN   L+ A++DTT+  +A+ FK+LS
Sbjct: 269 LQSG-SATATQDLLSVLLTFRDDKGTPLTNDEILDNFSSLLHASYDTTTSPMALIFKLLS 328

Query: 302 QHTNCYTLLLQEHTDIIRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAIS 361
            +  CY  ++QE  +I+ +K  G+ +T +D+K MKYTWQVA+E++R+FPP+FG+FRKAI+
Sbjct: 329 SNPECYQKVVQEQLEILSNKEEGEEITWKDLKAMKYTWQVAQETLRMFPPVFGTFRKAIT 388

Query: 362 DIEYESFLIPKGWKVLWTTYGTHYGEEYFEDPLSFKPSRFEEP---VSQYVYVPFGGGPR 421
           DI+Y+ + IPKGWK+LWTTY TH  + YF +P  F PSRF++    V+ Y ++PFGGG R
Sbjct: 389 DIQYDGYTIPKGWKLLWTTYSTHPKDLYFNEPEKFMPSRFDQEGKHVAPYTFLPFGGGQR 448

Query: 422 ACAGYQLAKLNILIFVHSVVTRY-DWSLVHPDEPIIMDPL-PVPYHGMPIKISPK 466
           +C G++ +K+ IL+FVH  V  +  ++ V PDE I  DPL P+P  G  IK+ P+
Sbjct: 449 SCVGWEFSKMEILLFVHHFVKTFSSYTPVDPDEKISGDPLPPLPSKGFSIKLFPR 498

[Close]

BLAST of CsGy6G022100 vs. ExPASy Swiss-Prot
Match: Q8W4T9 (Taxane 13-alpha-hydroxylase OS=Taxus cuspidata OX=99806 GN=CYP725A2 PE=1 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 4.9e-90
Identity = 175/470 (37.23%), Postives = 280/470 (59.57%), Query Frame = 0


Query: 8   ILLIISIAFVLRSTKRSR-NVPPGAMGLPWIGETMVFYRAQSKNRLFEDFVEPRVAKYGK 67
           + ++  I    RS + S   +PPG +G P +GET+ F R+   +   + F+E R++K+G 
Sbjct: 18  LAVMAGIILFFRSKRHSSVKLPPGNLGFPLVGETLQFVRSLGSSTP-QQFIEERMSKFGD 77

Query: 68  IFKTNLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVR 127
           +FKT+++G PTVV+ G   NR  LSNE KLV  SWPSS ++L+GE+C+  K GE+HR VR
Sbjct: 78  VFKTSIIGHPTVVLCGPAGNRLVLSNENKLVQMSWPSSMMKLIGEDCLGGKTGEQHRIVR 137

Query: 128 GLIAATLSTSSLDVMMPKLCNTIQVYLDTKWVGCDTIGLFHSAKVLTFTIVFECLLGI-- 187
             +   L   +L     K+ + IQ +++ KW G D   +    K L F++      GI  
Sbjct: 138 AALTRFLGPQALQNHFAKMSSGIQRHINEKWKGKDEATVLPLVKDLVFSVASRLFFGITE 197

Query: 188 -QVEPRVLTMFERVLEGVFAPPFRFPGTRFSRAKKARLEIETTLREVVREKRRKIEGKLE 247
             ++ ++  + E +L G F+ P   PG  + +A +AR  +   +  ++ ++R ++     
Sbjct: 198 EHLQEQLHNLLEVILVGSFSVPLNIPGFSYHKAIQARATLADIMTHLIEKRRNELRAGTA 257

Query: 248 EEECGSLLSRMVRAM--IGGEISEMEVIDNIVLLVFAAHDTTSFAIAMTFKMLSQHTNCY 307
            E    LLS ++      G  +++ E++DN  +L+  ++D+T+  + M  K+L+ H   Y
Sbjct: 258 SEN-QDLLSVLLTFTDERGNSLADKEILDNFSMLLHGSYDSTNSPLTMLIKVLASHPESY 317

Query: 308 TLLLQEHTDIIRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIEYES 367
             + QE   I+ +K  G+ +  +D+K+MKY+WQV +E++R++PPIFG+FRKAI+DI Y  
Sbjct: 318 EKVAQEQFGILSTKMEGEEIAWKDLKEMKYSWQVVQETLRMYPPIFGTFRKAITDIHYNG 377

Query: 368 FLIPKGWKVLWTTYGTHYGEEYFEDPLSFKPSRFEEP---VSQYVYVPFGGGPRACAGYQ 427
           + IPKGWK+LWTTY T   EEYF+D   FKPSRFEE    V+ Y Y+PFGGG R C G++
Sbjct: 378 YTIPKGWKLLWTTYSTQTKEEYFKDADQFKPSRFEEEGKHVTPYTYLPFGGGMRVCPGWE 437

Query: 428 LAKLNILIFVHSVVTRYD-WSLVHPDEPIIMDPL-PVPYHGMPIKISPKS 467
            AK+  L+F+H  V  +     + P+E +   PL P+P +G+PIK+  +S
Sbjct: 438 FAKMETLLFLHHFVKAFSGLKAIDPNEKLSGKPLPPLPVNGLPIKLYSRS 485

[Close]

BLAST of CsGy6G022100 vs. ExPASy Swiss-Prot
Match: Q50EK0 (Cytochrome P450 716B2 OS=Picea sitchensis OX=3332 GN=CYP716B2 PE=2 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 5.4e-89
Identity = 185/479 (38.62%), Postives = 272/479 (56.78%), Query Frame = 0


Query: 2   LSFLAAILLIISIAFVL-----RSTKRSRNVPPGAMGLPWIGETMVFYRAQSKNRLFEDF 61
           L F   +   I +AF         TK  R +PPG  G P IGET+ F   Q K     DF
Sbjct: 21  LMFTVFLASFIGLAFFFYLISNHRTKAWRGIPPGTFGWPLIGETLEFLGCQRKGNP-RDF 80

Query: 62  VEPRVAKYGKIFKTNLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSSSVQLMGEECIMQ 121
            + R  KYG +F T+L+G PTVV    E NRF  SNE KLVV+SWPSS   L     ++ 
Sbjct: 81  FDSRTQKYGNVFTTSLVGHPTVVFCSPEGNRFLFSNENKLVVNSWPSSVGNLF-RSSLIT 140

Query: 122 KEGEKHRCVRGLIAATLSTSSLDVMMPKLCNTIQVYLDTKWVGCDTIGLFHSAKVLTFTI 181
             G+  + +R ++   L   +L   + ++ +  + +L   W+G D +      K  TF++
Sbjct: 141 TVGDDAKRLRRILMTFLRPEALREFVGRVDSMTKRHLAEHWIGKDEVTALPLLKRYTFSL 200

Query: 182 VFECLLGIQVEP---RVLTMFERVLEGVFAPPFRFPGTRFSRAKKARLEIETTLREVVRE 241
             +    I  +    R+   F   ++GV   P   PGTR+++AK A   I   L  ++ E
Sbjct: 201 ACDLFASINNKDDLGRLWLHFMVFVKGVMQIPIDLPGTRYNKAKHAANAIRQQLGSIINE 260

Query: 242 KRRKIE---GKLEEEECGSLLSRMVRAMIGGEISEMEVIDNIVLLVFAAHDTTSFAIAMT 301
           ++  +E      E++    LLS +     G  +++ E+ DNI+LL++A HDT+S  + + 
Sbjct: 261 RKIGLEAGNASPEQDLLSFLLSNVDEQ--GESLTDNEIQDNILLLLYAGHDTSSSTLTVL 320

Query: 302 FKMLSQHTNCYTLLLQEHTDIIRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSF 361
            K L+++ +CY  +L+E  DI  SK  GQ L  ED+++MKY+W+VA+E++RLFP   GSF
Sbjct: 321 LKFLAENPHCYEEVLREQLDIAGSKEAGQLLEWEDLQRMKYSWRVAQEALRLFPAAQGSF 380

Query: 362 RKAISDIEYESFLIPKGWKVLWTTYGTHYGEEYFEDPLSFKPSRFE-EPVSQYVYVPFGG 421
           RKAI +  Y+ F IPKGWK+ WT   TH   EYF +P +F PSRFE E    Y +VPFGG
Sbjct: 381 RKAIKEFIYDGFTIPKGWKMYWTVNSTHRKSEYFSNPETFDPSRFEGEGPPPYTFVPFGG 440

Query: 422 GPRACAGYQLAKLNILIFVHSVVTRYDWSLVHPDEPIIMDPLPVPYHGMPIKISPKSQV 469
           GPR C G + A+L IL+F+H++V    W+LV+P E +I+DP+P P +G+PIK+ P   V
Sbjct: 441 GPRMCPGNEFARLEILVFLHNIVKNCKWNLVNPGEKVIVDPMPAPVNGLPIKLVPHDSV 495

[Close]

BLAST of CsGy6G022100 vs. ExPASy Swiss-Prot
Match: Q50EK1 (Cytochrome P450 716B1 OS=Picea sitchensis OX=3332 GN=CYP716B1 PE=2 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 2.1e-88
Identity = 182/475 (38.32%), Postives = 272/475 (57.26%), Query Frame = 0


Query: 2   LSFLAAILLIISIAFVL-----RSTKRSRNVPPGAMGLPWIGETMVFYRAQSKNRLFEDF 61
           L F   +   I +AF         TK  R +PPG  G P IGET+ F   Q K     DF
Sbjct: 21  LMFTVFLASFIGLAFFFYLISNHRTKAWRGIPPGTFGWPLIGETLEFLGCQRKGNP-RDF 80

Query: 62  VEPRVAKYGKIFKTNLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSSSVQLMGEECIMQ 121
            + R  KYG +F T+L+G PTVV    E NRF  SNE KLVV+SWPSS   L     ++ 
Sbjct: 81  FDSRTQKYGNVFTTSLVGHPTVVFCSPEGNRFLFSNENKLVVNSWPSSVGNLF-RSSLIT 140

Query: 122 KEGEKHRCVRGLIAATLSTSSLDVMMPKLCNTIQVYLDTKWVGCDTIGLFHSAKVLTFTI 181
             G+  + +R ++   L   +L   + ++ +  + +L   W+G D +      K  TF++
Sbjct: 141 TVGDDAKRLRRILMTFLRPEALREFVGRVDSMTKRHLAEHWIGKDEVMALPLLKRYTFSL 200

Query: 182 VFECLLGIQVE---PRVLTMFERVLEGVFAPPFRFPGTRFSRAKKARLEIETTLREVVRE 241
             +    I  +    R+   F   ++GV   P   PGTR+++ K A   I   L  ++ E
Sbjct: 201 ACDLFASINTKDDLDRLWLHFMVFVKGVMQIPIDLPGTRYNKTKHAANAIRQQLGSIINE 260

Query: 242 KRRKIE---GKLEEEECGSLLSRMVRAMIGGEISEMEVIDNIVLLVFAAHDTTSFAIAMT 301
           ++  +E      E++    LLS +     G  +++ E+ DNI+LL++A HDT+S  + + 
Sbjct: 261 RKIALEAGNASPEQDLLSFLLSNVDEQ--GESLTDNEIQDNILLLLYAGHDTSSSTLTVL 320

Query: 302 FKMLSQHTNCYTLLLQEHTDIIRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSF 361
            K L+++ +CY  +L+E  +I  SK  GQ L  ED+++MKY+W+VA+E++RLFP + GSF
Sbjct: 321 LKFLAENPHCYEEVLREQLNIAGSKEEGQLLEWEDLQRMKYSWRVAQEALRLFPAVQGSF 380

Query: 362 RKAISDIEYESFLIPKGWKVLWTTYGTHYGEEYFEDPLSFKPSRFE-EPVSQYVYVPFGG 421
           RKAI +  Y+ F IPKGWK+ WT   TH   EYF +P  F PSRFE E    Y +VPFGG
Sbjct: 381 RKAIKEFIYDGFTIPKGWKLHWTVNSTHQKSEYFSNPEKFDPSRFEGEGPPPYTFVPFGG 440

Query: 422 GPRACAGYQLAKLNILIFVHSVVTRYDWSLVHPDEPIIMDPLPVPYHGMPIKISP 465
           GPR C G + A++ ILIF+H++V  ++W+LV+P E +I+DP+P P +G+PIK+ P
Sbjct: 441 GPRMCPGNEFARMEILIFLHNIVKNFNWNLVNPLEKVIVDPMPAPVNGLPIKLVP 491

[Close]

BLAST of CsGy6G022100 vs. ExPASy Swiss-Prot
Match: Q84KI1 (Taxoid 14-beta-hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 2.3e-87
Identity = 183/477 (38.36%), Postives = 281/477 (58.91%), Query Frame = 0


Query: 1   MLSFLAAILLIISIAFVLRSTKRSR-NVPPGAMGLPWIGETMVFYRAQSKNRLFEDFVEP 60
           +LS +A I  ++ +   LRS +RS   +PPG +G P+IGE+++F +A   N + E F++ 
Sbjct: 25  VLSAVAGI--VLPLLLFLRSKRRSSVGLPPGKLGYPFIGESLLFLKALRSNTV-EQFLDE 84

Query: 61  RVAKYGKIFKTNLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSSSVQLMGEECIMQKEG 120
           RV  +G +FKT+L+G PTVV+ G   NR  L+NE KLV  SWP SS++LMGE+ I  K G
Sbjct: 85  RVKNFGNVFKTSLIGHPTVVLCGPAGNRLILANEEKLVQMSWPKSSMKLMGEKSITAKRG 144

Query: 121 EKHRCVRGLIAATLSTSSLDVMMPKLCNTIQVYLDTKWVGCDTIGLFHSAKVLTFTIVFE 180
           E H  +R  +    S  +L   + ++  TI+ +++ KW G D + +      L F I   
Sbjct: 145 EGHMIIRSALQGFFSPGALQKYIGQMSKTIENHINEKWKGNDQVSVVALVGDLVFDISAC 204

Query: 181 CLLGIQVE---PRVLTMFERVLEGVFAPPFRFPGTRFSRAKKARLEIETTLREVVREKRR 240
               I  +    R+  + E +  GV A P   PG  + RA +AR ++   L  ++ +++ 
Sbjct: 205 LFFNINEKHERERLFELLEIIAVGVLAVPVDLPGFAYHRALQARSKLNAILSGLIEKRKM 264

Query: 241 KIEGKLEEEECGSLLSRMV--RAMIGGEISEMEVIDNIVLLVFAAHDTTSFAIAMTFKML 300
            +   L       LLS  +  +   G   S+ E++DN   L+  ++DTT  A+A  FK+L
Sbjct: 265 DLSSGLATSN-QDLLSVFLTFKDDRGNPCSDEEILDNFSGLLHGSYDTTVSAMACVFKLL 324

Query: 301 SQHTNCYTLLLQEHTDIIRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAI 360
           S +  CY  ++QE   I+ +K  G  +T +D+K MKYTWQV +E++RL+P IFGSFR+AI
Sbjct: 325 SSNPECYEKVVQEQLGILSNKLEGDEITWKDVKSMKYTWQVVQETLRLYPSIFGSFRQAI 384

Query: 361 SDIEYESFLIPKGWKVLWTTYGTHYGEEYFEDPLSFKPSRFEEP---VSQYVYVPFGGGP 420
           +DI Y  ++IPKGWK+LWT Y TH  E YF +P  F PSRF++    V+ Y ++PFGGG 
Sbjct: 385 TDIHYNGYIIPKGWKLLWTPYTTHPKEMYFSEPEKFLPSRFDQEGKLVAPYTFLPFGGGQ 444

Query: 421 RACAGYQLAKLNILIFVHSVVTRYD-WSLVHPDEPIIMDPL-PVPYHGMPIKISPKS 467
           R+C G++ +K+ IL+ VH  V  +  ++ V P E I  D L P+P +G  +K+ P+S
Sbjct: 445 RSCPGWEFSKMEILLSVHHFVKTFSTFTPVDPAEIIARDSLCPLPSNGFSVKLFPRS 497

[Close]

BLAST of CsGy6G022100 vs. NCBI nr
Match: XP_004140249.1 (taxadiene 5-alpha hydroxylase [Cucumis sativus] >KAE8647348.1 hypothetical protein Csa_003098 [Cucumis sativus])

HSP 1 Score: 948 bits (2450), Expect = 0.0
Identity = 475/475 (100.00%), Postives = 475/475 (100.00%), Query Frame = 0


Query: 1   MLSFLAAILLIISIAFVLRSTKRSRNVPPGAMGLPWIGETMVFYRAQSKNRLFEDFVEPR 60
           MLSFLAAILLIISIAFVLRSTKRSRNVPPGAMGLPWIGETMVFYRAQSKNRLFEDFVEPR
Sbjct: 1   MLSFLAAILLIISIAFVLRSTKRSRNVPPGAMGLPWIGETMVFYRAQSKNRLFEDFVEPR 60

Query: 61  VAKYGKIFKTNLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSSSVQLMGEECIMQKEGE 120
           VAKYGKIFKTNLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSSSVQLMGEECIMQKEGE
Sbjct: 61  VAKYGKIFKTNLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSSSVQLMGEECIMQKEGE 120

Query: 121 KHRCVRGLIAATLSTSSLDVMMPKLCNTIQVYLDTKWVGCDTIGLFHSAKVLTFTIVFEC 180
           KHRCVRGLIAATLSTSSLDVMMPKLCNTIQVYLDTKWVGCDTIGLFHSAKVLTFTIVFEC
Sbjct: 121 KHRCVRGLIAATLSTSSLDVMMPKLCNTIQVYLDTKWVGCDTIGLFHSAKVLTFTIVFEC 180

Query: 181 LLGIQVEPRVLTMFERVLEGVFAPPFRFPGTRFSRAKKARLEIETTLREVVREKRRKIEG 240
           LLGIQVEPRVLTMFERVLEGVFAPPFRFPGTRFSRAKKARLEIETTLREVVREKRRKIEG
Sbjct: 181 LLGIQVEPRVLTMFERVLEGVFAPPFRFPGTRFSRAKKARLEIETTLREVVREKRRKIEG 240

Query: 241 KLEEEECGSLLSRMVRAMIGGEISEMEVIDNIVLLVFAAHDTTSFAIAMTFKMLSQHTNC 300
           KLEEEECGSLLSRMVRAMIGGEISEMEVIDNIVLLVFAAHDTTSFAIAMTFKMLSQHTNC
Sbjct: 241 KLEEEECGSLLSRMVRAMIGGEISEMEVIDNIVLLVFAAHDTTSFAIAMTFKMLSQHTNC 300

Query: 301 YTLLLQEHTDIIRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIEYE 360
           YTLLLQEHTDIIRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIEYE
Sbjct: 301 YTLLLQEHTDIIRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIEYE 360

Query: 361 SFLIPKGWKVLWTTYGTHYGEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLA 420
           SFLIPKGWKVLWTTYGTHYGEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLA
Sbjct: 361 SFLIPKGWKVLWTTYGTHYGEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLA 420

Query: 421 KLNILIFVHSVVTRYDWSLVHPDEPIIMDPLPVPYHGMPIKISPKSQVCSNVHQM 475
           KLNILIFVHSVVTRYDWSLVHPDEPIIMDPLPVPYHGMPIKISPKSQVCSNVHQM
Sbjct: 421 KLNILIFVHSVVTRYDWSLVHPDEPIIMDPLPVPYHGMPIKISPKSQVCSNVHQM 475

[Close]

BLAST of CsGy6G022100 vs. NCBI nr
Match: XP_008449518.1 (PREDICTED: taxadiene 5-alpha hydroxylase [Cucumis melo])

HSP 1 Score: 904 bits (2337), Expect = 0.0
Identity = 451/476 (94.75%), Postives = 463/476 (97.27%), Query Frame = 0


Query: 1   MLSFLAAILLIISIAFVLRSTKRSRNVPPGAMGLPWIGETMVFYRAQSKNRLFEDFVEPR 60
           MLSFLAAILLII+IAFVLRS KRS+NVPPG MGLPWIGETM FYRAQSKNRLFEDFVEPR
Sbjct: 1   MLSFLAAILLIITIAFVLRSNKRSKNVPPGEMGLPWIGETMAFYRAQSKNRLFEDFVEPR 60

Query: 61  VAKYGKIFKTNLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSSSVQLMGEECIMQKEGE 120
           VAKYGKIFKTNLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSSSVQLMGEECIMQKEGE
Sbjct: 61  VAKYGKIFKTNLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSSSVQLMGEECIMQKEGE 120

Query: 121 KHRCVRGLIAATLSTSSLDVMMPKLCNTIQVYLDTKWVGCDTIGLFHSAKVLTFTIVFEC 180
           KHRCVRGLIA+ LSTSSL+VMMPKLCNTIQVYLDTKWV CDT+GLF SAKVLTFTIVFEC
Sbjct: 121 KHRCVRGLIASALSTSSLEVMMPKLCNTIQVYLDTKWVDCDTVGLFRSAKVLTFTIVFEC 180

Query: 181 LLGIQVEPRVLTMFERVLEGVFAPPFRFPGTRFSRAKKARLEIETTLREVVREKRRKIEG 240
           LLGI+VEPRVLT FERVLEGVFAPPFRFPGTRFSRAKKARLEIET LREVVREKRRKIEG
Sbjct: 181 LLGIKVEPRVLTTFERVLEGVFAPPFRFPGTRFSRAKKARLEIETMLREVVREKRRKIEG 240

Query: 241 KLEEEE-CGSLLSRMVRAMIGGEISEMEVIDNIVLLVFAAHDTTSFAIAMTFKMLSQHTN 300
           KLEEEE CGSLLSRMVRAMIGGEISEMEV+DNIVLLVFAAHDTTSFAIAMTFKMLSQHTN
Sbjct: 241 KLEEEEQCGSLLSRMVRAMIGGEISEMEVVDNIVLLVFAAHDTTSFAIAMTFKMLSQHTN 300

Query: 301 CYTLLLQEHTDIIRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIEY 360
           CY+LLLQEH DI+RSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIEY
Sbjct: 301 CYSLLLQEHADIVRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIEY 360

Query: 361 ESFLIPKGWKVLWTTYGTHYGEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQL 420
           ESFLIPKGWKVLWTTYGTHYGEEYF DPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQL
Sbjct: 361 ESFLIPKGWKVLWTTYGTHYGEEYFGDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQL 420

Query: 421 AKLNILIFVHSVVTRYDWSLVHPDEPIIMDPLPVPYHGMPIKISPKSQVCSNVHQM 475
           AKLNILIF+HSVVTRYDWSLVHPDEPIIMDPLPVP HGMPIKISPKS++C NVHQM
Sbjct: 421 AKLNILIFIHSVVTRYDWSLVHPDEPIIMDPLPVPCHGMPIKISPKSEICPNVHQM 476

[Close]

BLAST of CsGy6G022100 vs. NCBI nr
Match: XP_038888983.1 (taxadiene 5-alpha hydroxylase [Benincasa hispida])

HSP 1 Score: 836 bits (2159), Expect = 9.85e-304
Identity = 423/476 (88.87%), Postives = 443/476 (93.07%), Query Frame = 0


Query: 1   MLSFLAAILLIISIAFVLRSTKRSRNVPPGAMGLPWIGETMVFYRAQSKNRLFEDFVEPR 60
           MLSF AA +LIISIAFV+RS KRS+NVPPG MGLPWIGETM FYRAQSKNRLFEDFVEPR
Sbjct: 1   MLSFFAA-MLIISIAFVIRSKKRSKNVPPGQMGLPWIGETMAFYRAQSKNRLFEDFVEPR 60

Query: 61  VAKYGKIFKTNLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSSSVQLMGEECIMQKEGE 120
           VAKYGKIFKT+LMGSPTVVVNGAEANRFFLSNEFKLVVSSWPS+SVQLMGEECIMQK+GE
Sbjct: 61  VAKYGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGE 120

Query: 121 KHRCVRGLIAATLSTSSLDVMMPKLCNTIQVYLDTKWVGCDTIGLFHSAKVLTFTIVFEC 180
            HR VR LIAA+LSTSSL+VMMPKL  TI+VYLDTKWVG DTIGLFHSAKVLTFT+VFEC
Sbjct: 121 SHRFVRSLIAASLSTSSLEVMMPKLFTTIRVYLDTKWVGRDTIGLFHSAKVLTFTVVFEC 180

Query: 181 LLGIQVEPRVLTMFERVLEGVFAPPFRFPGTRFSRAKKARLEIETTLREVVREKRRKIEG 240
           L+GI+VEPRVLT FERVLEGVFAPP RFPGTRFSRAKKARLEIE  LREVVREKRR+IEG
Sbjct: 181 LMGIKVEPRVLTTFERVLEGVFAPPIRFPGTRFSRAKKARLEIEKMLREVVREKRRRIEG 240

Query: 241 KLEEEE-CGSLLSRMVRAMIGGEISEMEVIDNIVLLVFAAHDTTSFAIAMTFKMLSQHTN 300
           KLEEEE CGSLLSRMVRAMI GEISEMEVIDN+VL VFAAHDTTSFAIAMTFKML+ HTN
Sbjct: 241 KLEEEEECGSLLSRMVRAMIRGEISEMEVIDNVVLFVFAAHDTTSFAIAMTFKMLAHHTN 300

Query: 301 CYTLLLQEHTDIIRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIEY 360
           CY+LLLQE TDI RSKG  QNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIEY
Sbjct: 301 CYSLLLQEQTDIARSKGSNQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIEY 360

Query: 361 ESFLIPKGWKVLWTTYGTHYGEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQL 420
           E F IP+GWKVLWTTYGTHY EEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQL
Sbjct: 361 ERFFIPEGWKVLWTTYGTHYKEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQL 420

Query: 421 AKLNILIFVHSVVTRYDWSLVHPDEPIIMDPLPVPYHGMPIKISPKSQVCSNVHQM 475
           AKLNILIF+H VVTRYDW+LVHPDEPIIMDPLPVP  GMP+KIS KS VC N HQM
Sbjct: 421 AKLNILIFIHLVVTRYDWNLVHPDEPIIMDPLPVPSQGMPMKISLKSDVCPNDHQM 475

[Close]

BLAST of CsGy6G022100 vs. NCBI nr
Match: KAG7012036.1 (hypothetical protein SDJN02_26944, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 805 bits (2080), Expect = 1.60e-291
Identity = 400/472 (84.75%), Postives = 431/472 (91.31%), Query Frame = 0


Query: 4   FLAAILLIISIAFVLRSTKRSRNVPPGAMGLPWIGETMVFYRAQSKNRLFEDFVEPRVAK 63
           FL+AIL IISI FV RS K S+ VPPG MGLPWIGETM FYRAQ KNRLFE+FV PRVAK
Sbjct: 16  FLSAIL-IISIVFVFRSIKGSKKVPPGQMGLPWIGETMAFYRAQRKNRLFEEFVGPRVAK 75

Query: 64  YGKIFKTNLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSSSVQLMGEECIMQKEGEKHR 123
           YGKIFKT+LMGSPTVVVNGAEANRFFLSNEFKLVVSSWPS+SVQLMGEECIMQK+GE HR
Sbjct: 76  YGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGESHR 135

Query: 124 CVRGLIAATLSTSSLDVMMPKLCNTIQVYLDTKWVGCDTIGLFHSAKVLTFTIVFECLLG 183
           C RGLIAA+L++SSL+ MMPKLC TIQ+YLDTKWVG D I L+HSAKVLTFTIVFECL+G
Sbjct: 136 CARGLIAASLASSSLEAMMPKLCTTIQLYLDTKWVGHDIISLYHSAKVLTFTIVFECLMG 195

Query: 184 IQVEPRVLTMFERVLEGVFAPPFRFPGTRFSRAKKARLEIETTLREVVREKRRKIEGKLE 243
           I+VEPR+LT FERVLEGVFAPPF FPGTRFSRAKKAR+EI   LREVVREKRR+IE + E
Sbjct: 196 IKVEPRMLTTFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLREVVREKRRRIEEEPE 255

Query: 244 EEECGSLLSRMVRAMIGGEISEMEVIDNIVLLVFAAHDTTSFAIAMTFKMLSQHTNCYTL 303
            EEC  LLSRMVRAMI GEISE+EV+DN+VLLVFAAHDTTSFAIA+TFKML+Q  NCY+L
Sbjct: 256 GEECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAVTFKMLAQQPNCYSL 315

Query: 304 LLQEHTDIIRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIEYESFL 363
           LLQEH DI+RSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAI+DIEYE F 
Sbjct: 316 LLQEHADIVRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITDIEYEGFF 375

Query: 364 IPKGWKVLWTTYGTHYGEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLN 423
           IPKGWKVLWTTYGTH  +EYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLN
Sbjct: 376 IPKGWKVLWTTYGTHLKDEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLN 435

Query: 424 ILIFVHSVVTRYDWSLVHPDEPIIMDPLPVPYHGMPIKISPKSQVCSNVHQM 475
           ILIF+H VVTRYDWSL+HPDEPIIMDPLP+P HGMPIKISPKS+   NV QM
Sbjct: 436 ILIFIHLVVTRYDWSLIHPDEPIIMDPLPIPSHGMPIKISPKSESHPNVQQM 486

[Close]

BLAST of CsGy6G022100 vs. NCBI nr
Match: XP_023554746.1 (taxadiene 5-alpha hydroxylase isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 804 bits (2076), Expect = 6.52e-291
Identity = 400/472 (84.75%), Postives = 431/472 (91.31%), Query Frame = 0


Query: 4   FLAAILLIISIAFVLRSTKRSRNVPPGAMGLPWIGETMVFYRAQSKNRLFEDFVEPRVAK 63
           FL+AIL IISI FV RS K S+ VPPG MGLPWIGETM FYRAQ KNRLFE+FV PRVAK
Sbjct: 16  FLSAIL-IISIIFVFRSIKGSKKVPPGQMGLPWIGETMAFYRAQRKNRLFEEFVGPRVAK 75

Query: 64  YGKIFKTNLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSSSVQLMGEECIMQKEGEKHR 123
           YGKIFKT+LMGSPTVVVNGAEANRFFLSNEFKLVVSSWPS+SVQLMGEECIMQK+GE HR
Sbjct: 76  YGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGESHR 135

Query: 124 CVRGLIAATLSTSSLDVMMPKLCNTIQVYLDTKWVGCDTIGLFHSAKVLTFTIVFECLLG 183
           CVRGLIAA+L++SSL+ MMPKLC TIQ+YLDTKW+G D I L+HSAKVLTFTIVFECL+G
Sbjct: 136 CVRGLIAASLASSSLEAMMPKLCTTIQLYLDTKWLGHDIISLYHSAKVLTFTIVFECLMG 195

Query: 184 IQVEPRVLTMFERVLEGVFAPPFRFPGTRFSRAKKARLEIETTLREVVREKRRKIEGKLE 243
           I+VEPR+LT FERVLEGVFAPPF FPGTRFSRAKKAR+EI   LREVVREKRR+IE + E
Sbjct: 196 IKVEPRMLTTFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLREVVREKRRRIEEEPE 255

Query: 244 EEECGSLLSRMVRAMIGGEISEMEVIDNIVLLVFAAHDTTSFAIAMTFKMLSQHTNCYTL 303
           EEEC  LLSRMVRAMI GEISE+EV+DN+VLLVFAAHDTTSFAIAMTFKML+Q  NCY+L
Sbjct: 256 EEECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAMTFKMLAQQPNCYSL 315

Query: 304 LLQEHTDIIRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIEYESFL 363
           LLQEH DI+RSKG GQN+TMEDIKKMKYTWQVARESIRLFPPIFGSFRKAI+DIEYE F 
Sbjct: 316 LLQEHADIVRSKGAGQNMTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITDIEYEGFF 375

Query: 364 IPKGWKVLWTTYGTHYGEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLN 423
           IPKGWKVLWTTYGTH  +EYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLN
Sbjct: 376 IPKGWKVLWTTYGTHLEDEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLN 435

Query: 424 ILIFVHSVVTRYDWSLVHPDEPIIMDPLPVPYHGMPIKISPKSQVCSNVHQM 475
           ILIF+H VVTRYDWSLVHPDE IIMDPLP+P HGMPIKISPKS+   NV QM
Sbjct: 436 ILIFIHLVVTRYDWSLVHPDETIIMDPLPIPSHGMPIKISPKSEARPNVQQM 486

[Close]

BLAST of CsGy6G022100 vs. ExPASy TrEMBL
Match: A0A1S3BLK9 (taxadiene 5-alpha hydroxylase OS=Cucumis melo OX=3656 GN=LOC103491382 PE=3 SV=1)

HSP 1 Score: 904 bits (2337), Expect = 0.0
Identity = 451/476 (94.75%), Postives = 463/476 (97.27%), Query Frame = 0


Query: 1   MLSFLAAILLIISIAFVLRSTKRSRNVPPGAMGLPWIGETMVFYRAQSKNRLFEDFVEPR 60
           MLSFLAAILLII+IAFVLRS KRS+NVPPG MGLPWIGETM FYRAQSKNRLFEDFVEPR
Sbjct: 1   MLSFLAAILLIITIAFVLRSNKRSKNVPPGEMGLPWIGETMAFYRAQSKNRLFEDFVEPR 60

Query: 61  VAKYGKIFKTNLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSSSVQLMGEECIMQKEGE 120
           VAKYGKIFKTNLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSSSVQLMGEECIMQKEGE
Sbjct: 61  VAKYGKIFKTNLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSSSVQLMGEECIMQKEGE 120

Query: 121 KHRCVRGLIAATLSTSSLDVMMPKLCNTIQVYLDTKWVGCDTIGLFHSAKVLTFTIVFEC 180
           KHRCVRGLIA+ LSTSSL+VMMPKLCNTIQVYLDTKWV CDT+GLF SAKVLTFTIVFEC
Sbjct: 121 KHRCVRGLIASALSTSSLEVMMPKLCNTIQVYLDTKWVDCDTVGLFRSAKVLTFTIVFEC 180

Query: 181 LLGIQVEPRVLTMFERVLEGVFAPPFRFPGTRFSRAKKARLEIETTLREVVREKRRKIEG 240
           LLGI+VEPRVLT FERVLEGVFAPPFRFPGTRFSRAKKARLEIET LREVVREKRRKIEG
Sbjct: 181 LLGIKVEPRVLTTFERVLEGVFAPPFRFPGTRFSRAKKARLEIETMLREVVREKRRKIEG 240

Query: 241 KLEEEE-CGSLLSRMVRAMIGGEISEMEVIDNIVLLVFAAHDTTSFAIAMTFKMLSQHTN 300
           KLEEEE CGSLLSRMVRAMIGGEISEMEV+DNIVLLVFAAHDTTSFAIAMTFKMLSQHTN
Sbjct: 241 KLEEEEQCGSLLSRMVRAMIGGEISEMEVVDNIVLLVFAAHDTTSFAIAMTFKMLSQHTN 300

Query: 301 CYTLLLQEHTDIIRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIEY 360
           CY+LLLQEH DI+RSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIEY
Sbjct: 301 CYSLLLQEHADIVRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIEY 360

Query: 361 ESFLIPKGWKVLWTTYGTHYGEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQL 420
           ESFLIPKGWKVLWTTYGTHYGEEYF DPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQL
Sbjct: 361 ESFLIPKGWKVLWTTYGTHYGEEYFGDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQL 420

Query: 421 AKLNILIFVHSVVTRYDWSLVHPDEPIIMDPLPVPYHGMPIKISPKSQVCSNVHQM 475
           AKLNILIF+HSVVTRYDWSLVHPDEPIIMDPLPVP HGMPIKISPKS++C NVHQM
Sbjct: 421 AKLNILIFIHSVVTRYDWSLVHPDEPIIMDPLPVPCHGMPIKISPKSEICPNVHQM 476

[Close]

BLAST of CsGy6G022100 vs. ExPASy TrEMBL
Match: A0A0A0KEB2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G423370 PE=3 SV=1)

HSP 1 Score: 897 bits (2317), Expect = 0.0
Identity = 444/444 (100.00%), Postives = 444/444 (100.00%), Query Frame = 0


Query: 32  MGLPWIGETMVFYRAQSKNRLFEDFVEPRVAKYGKIFKTNLMGSPTVVVNGAEANRFFLS 91
           MGLPWIGETMVFYRAQSKNRLFEDFVEPRVAKYGKIFKTNLMGSPTVVVNGAEANRFFLS
Sbjct: 1   MGLPWIGETMVFYRAQSKNRLFEDFVEPRVAKYGKIFKTNLMGSPTVVVNGAEANRFFLS 60

Query: 92  NEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIAATLSTSSLDVMMPKLCNTIQV 151
           NEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIAATLSTSSLDVMMPKLCNTIQV
Sbjct: 61  NEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIAATLSTSSLDVMMPKLCNTIQV 120

Query: 152 YLDTKWVGCDTIGLFHSAKVLTFTIVFECLLGIQVEPRVLTMFERVLEGVFAPPFRFPGT 211
           YLDTKWVGCDTIGLFHSAKVLTFTIVFECLLGIQVEPRVLTMFERVLEGVFAPPFRFPGT
Sbjct: 121 YLDTKWVGCDTIGLFHSAKVLTFTIVFECLLGIQVEPRVLTMFERVLEGVFAPPFRFPGT 180

Query: 212 RFSRAKKARLEIETTLREVVREKRRKIEGKLEEEECGSLLSRMVRAMIGGEISEMEVIDN 271
           RFSRAKKARLEIETTLREVVREKRRKIEGKLEEEECGSLLSRMVRAMIGGEISEMEVIDN
Sbjct: 181 RFSRAKKARLEIETTLREVVREKRRKIEGKLEEEECGSLLSRMVRAMIGGEISEMEVIDN 240

Query: 272 IVLLVFAAHDTTSFAIAMTFKMLSQHTNCYTLLLQEHTDIIRSKGPGQNLTMEDIKKMKY 331
           IVLLVFAAHDTTSFAIAMTFKMLSQHTNCYTLLLQEHTDIIRSKGPGQNLTMEDIKKMKY
Sbjct: 241 IVLLVFAAHDTTSFAIAMTFKMLSQHTNCYTLLLQEHTDIIRSKGPGQNLTMEDIKKMKY 300

Query: 332 TWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWKVLWTTYGTHYGEEYFEDPLSFK 391
           TWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWKVLWTTYGTHYGEEYFEDPLSFK
Sbjct: 301 TWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWKVLWTTYGTHYGEEYFEDPLSFK 360

Query: 392 PSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFVHSVVTRYDWSLVHPDEPIIMDPL 451
           PSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFVHSVVTRYDWSLVHPDEPIIMDPL
Sbjct: 361 PSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFVHSVVTRYDWSLVHPDEPIIMDPL 420

Query: 452 PVPYHGMPIKISPKSQVCSNVHQM 475
           PVPYHGMPIKISPKSQVCSNVHQM
Sbjct: 421 PVPYHGMPIKISPKSQVCSNVHQM 444

[Close]

BLAST of CsGy6G022100 vs. ExPASy TrEMBL
Match: A0A6J1GJY9 (taxadiene 5-alpha hydroxylase isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111455006 PE=3 SV=1)

HSP 1 Score: 801 bits (2069), Expect = 3.68e-290
Identity = 399/472 (84.53%), Postives = 431/472 (91.31%), Query Frame = 0


Query: 4   FLAAILLIISIAFVLRSTKRSRNVPPGAMGLPWIGETMVFYRAQSKNRLFEDFVEPRVAK 63
           FL+AIL IISI FV RS K S+ VPPG MGLPWIGETMVFYRAQ KNRLFE+FV PRVAK
Sbjct: 16  FLSAIL-IISIIFVFRSIKGSKKVPPGQMGLPWIGETMVFYRAQRKNRLFEEFVGPRVAK 75

Query: 64  YGKIFKTNLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSSSVQLMGEECIMQKEGEKHR 123
           YGKIFKT+LMGSPTVVVNGAEANRFFLSNEFKLVVSSWPS+SVQLMGEECIMQK+GE HR
Sbjct: 76  YGKIFKTSLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGESHR 135

Query: 124 CVRGLIAATLSTSSLDVMMPKLCNTIQVYLDTKWVGCDTIGLFHSAKVLTFTIVFECLLG 183
           CVRGLIAA+L++SSL+ MMPKLC TIQ+YLDTKW+G D I L+HSAKVLTFTIVFECL+G
Sbjct: 136 CVRGLIAASLASSSLEAMMPKLCTTIQLYLDTKWLGHDIISLYHSAKVLTFTIVFECLMG 195

Query: 184 IQVEPRVLTMFERVLEGVFAPPFRFPGTRFSRAKKARLEIETTLREVVREKRRKIEGKLE 243
           I+VEPR+LT FERVLEGVFAPPF FPGTRFSRAKKAR+EI   LREVVREKRR+IE +  
Sbjct: 196 IKVEPRMLTTFERVLEGVFAPPFSFPGTRFSRAKKARMEIGKMLREVVREKRRRIEEEPV 255

Query: 244 EEECGSLLSRMVRAMIGGEISEMEVIDNIVLLVFAAHDTTSFAIAMTFKMLSQHTNCYTL 303
           EEEC  LLSRMVRAMI GEISE+EV+DN+VLLVFAAHDTTSFAIAMTFKML+Q  NCY+L
Sbjct: 256 EEECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAMTFKMLAQQPNCYSL 315

Query: 304 LLQEHTDIIRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIEYESFL 363
           LLQEH DI+RSKG GQN+TMEDIKKMKYTWQVARESIRLFPPIFGSFRKAI+DIEYE F 
Sbjct: 316 LLQEHADIVRSKGAGQNMTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITDIEYEGFF 375

Query: 364 IPKGWKVLWTTYGTHYGEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLN 423
           IPKGWKVLWTTYGTH  ++YFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLN
Sbjct: 376 IPKGWKVLWTTYGTHLEDKYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLN 435

Query: 424 ILIFVHSVVTRYDWSLVHPDEPIIMDPLPVPYHGMPIKISPKSQVCSNVHQM 475
           ILIF+H VVTRYDWSLVHPDE IIMDPLP+P HGMPIKISPKS+   NV QM
Sbjct: 436 ILIFIHLVVTRYDWSLVHPDETIIMDPLPIPSHGMPIKISPKSEARPNVQQM 486

[Close]

BLAST of CsGy6G022100 vs. ExPASy TrEMBL
Match: A0A6J1HZM9 (taxadiene 5-alpha hydroxylase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111468378 PE=3 SV=1)

HSP 1 Score: 795 bits (2053), Expect = 6.41e-288
Identity = 395/472 (83.69%), Postives = 430/472 (91.10%), Query Frame = 0


Query: 4   FLAAILLIISIAFVLRSTKRSRNVPPGAMGLPWIGETMVFYRAQSKNRLFEDFVEPRVAK 63
           FL+AIL IISI FV RS K S+ VPPG MGLPWIG+TM FYRAQ KNRLFEDFV PRVAK
Sbjct: 4   FLSAIL-IISIIFVFRSIKGSKKVPPGQMGLPWIGQTMAFYRAQRKNRLFEDFVGPRVAK 63

Query: 64  YGKIFKTNLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSSSVQLMGEECIMQKEGEKHR 123
           YGKIFKT+LMGSPTVV+NGAEANRFFLSNEFKLVVSSWP++SVQLMGEECIMQK+GE HR
Sbjct: 64  YGKIFKTSLMGSPTVVINGAEANRFFLSNEFKLVVSSWPAASVQLMGEECIMQKQGESHR 123

Query: 124 CVRGLIAATLSTSSLDVMMPKLCNTIQVYLDTKWVGCDTIGLFHSAKVLTFTIVFECLLG 183
           CVRGLIAA+L++SSL+ MMPKLC TIQ++LDTKWVG D I L+HSAKVLTF+IVFECL+G
Sbjct: 124 CVRGLIAASLASSSLEAMMPKLCTTIQLHLDTKWVGHDIISLYHSAKVLTFSIVFECLMG 183

Query: 184 IQVEPRVLTMFERVLEGVFAPPFRFPGTRFSRAKKARLEIETTLREVVREKRRKIEGKLE 243
           I+VEPR+LT FERVLEGVFA PF FPGTRFSRAKKAR+EI   LREVVREKRR+IE +LE
Sbjct: 184 IKVEPRMLTTFERVLEGVFALPFSFPGTRFSRAKKARMEIGKMLREVVREKRRRIEEELE 243

Query: 244 EEECGSLLSRMVRAMIGGEISEMEVIDNIVLLVFAAHDTTSFAIAMTFKMLSQHTNCYTL 303
           EEEC  LLSRMVRAMI GEISE+EV+DN+VLLVFAAHDTTSFAIAMTFKML+QH +CY+L
Sbjct: 244 EEECELLLSRMVRAMIRGEISEVEVVDNVVLLVFAAHDTTSFAIAMTFKMLAQHPSCYSL 303

Query: 304 LLQEHTDIIRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIEYESFL 363
           LLQEH DI+RSKG GQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAI+DIEYE F 
Sbjct: 304 LLQEHADIVRSKGAGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITDIEYEGFF 363

Query: 364 IPKGWKVLWTTYGTHYGEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLN 423
           IPKGWKVLWT YGTH  +EYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLN
Sbjct: 364 IPKGWKVLWTMYGTHLEDEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLN 423

Query: 424 ILIFVHSVVTRYDWSLVHPDEPIIMDPLPVPYHGMPIKISPKSQVCSNVHQM 475
           ILIF+H VVTRYDWSL+HPDE IIMDPLP+P HGM  KISPKS+V  NV QM
Sbjct: 424 ILIFIHLVVTRYDWSLIHPDETIIMDPLPIPSHGMQTKISPKSEVRPNVQQM 474

[Close]

BLAST of CsGy6G022100 vs. ExPASy TrEMBL
Match: A0A6J1GK43 (taxadiene 5-alpha hydroxylase isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111455006 PE=3 SV=1)

HSP 1 Score: 791 bits (2042), Expect = 3.03e-286
Identity = 388/457 (84.90%), Postives = 419/457 (91.68%), Query Frame = 0


Query: 19  RSTKRSRNVPPGAMGLPWIGETMVFYRAQSKNRLFEDFVEPRVAKYGKIFKTNLMGSPTV 78
           RS K S+ VPPG MGLPWIGETMVFYRAQ KNRLFE+FV PRVAKYGKIFKT+LMGSPTV
Sbjct: 18  RSIKGSKKVPPGQMGLPWIGETMVFYRAQRKNRLFEEFVGPRVAKYGKIFKTSLMGSPTV 77

Query: 79  VVNGAEANRFFLSNEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIAATLSTSSL 138
           VVNGAEANRFFLSNEFKLVVSSWPS+SVQLMGEECIMQK+GE HRCVRGLIAA+L++SSL
Sbjct: 78  VVNGAEANRFFLSNEFKLVVSSWPSASVQLMGEECIMQKQGESHRCVRGLIAASLASSSL 137

Query: 139 DVMMPKLCNTIQVYLDTKWVGCDTIGLFHSAKVLTFTIVFECLLGIQVEPRVLTMFERVL 198
           + MMPKLC TIQ+YLDTKW+G D I L+HSAKVLTFTIVFECL+GI+VEPR+LT FERVL
Sbjct: 138 EAMMPKLCTTIQLYLDTKWLGHDIISLYHSAKVLTFTIVFECLMGIKVEPRMLTTFERVL 197

Query: 199 EGVFAPPFRFPGTRFSRAKKARLEIETTLREVVREKRRKIEGKLEEEECGSLLSRMVRAM 258
           EGVFAPPF FPGTRFSRAKKAR+EI   LREVVREKRR+IE +  EEEC  LLSRMVRAM
Sbjct: 198 EGVFAPPFSFPGTRFSRAKKARMEIGKMLREVVREKRRRIEEEPVEEECELLLSRMVRAM 257

Query: 259 IGGEISEMEVIDNIVLLVFAAHDTTSFAIAMTFKMLSQHTNCYTLLLQEHTDIIRSKGPG 318
           I GEISE+EV+DN+VLLVFAAHDTTSFAIAMTFKML+Q  NCY+LLLQEH DI+RSKG G
Sbjct: 258 IRGEISEVEVVDNVVLLVFAAHDTTSFAIAMTFKMLAQQPNCYSLLLQEHADIVRSKGAG 317

Query: 319 QNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWKVLWTTYGTH 378
           QN+TMEDIKKMKYTWQVARESIRLFPPIFGSFRKAI+DIEYE F IPKGWKVLWTTYGTH
Sbjct: 318 QNMTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAITDIEYEGFFIPKGWKVLWTTYGTH 377

Query: 379 YGEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFVHSVVTRYDWS 438
             ++YFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIF+H VVTRYDWS
Sbjct: 378 LEDKYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFIHLVVTRYDWS 437

Query: 439 LVHPDEPIIMDPLPVPYHGMPIKISPKSQVCSNVHQM 475
           LVHPDE IIMDPLP+P HGMPIKISPKS+   NV QM
Sbjct: 438 LVHPDETIIMDPLPIPSHGMPIKISPKSEARPNVQQM 474

[Close]

BLAST of CsGy6G022100 vs. TAIR 10
Match: AT2G42850.1 (cytochrome P450, family 718 )

HSP 1 Score: 604.4 bits (1557), Expect = 8.1e-173
Identity = 287/450 (63.78%), Postives = 362/450 (80.44%), Query Frame = 0


Query: 16  FVLRSTKRSRNVPPGAMGLPWIGETMVFYRAQSKNRLFEDFVEPRVAKYGKIFKTNLMGS 75
           F+ +  ++ + + PG MGLPWIGETM FY+AQ  NR+FEDFV PR+ K+G IFKT +MGS
Sbjct: 34  FITKKIQKKKKLLPGEMGLPWIGETMDFYKAQKSNRVFEDFVNPRIIKHGNIFKTRIMGS 93

Query: 76  PTVVVNGAEANRFFLSNEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRGLIAATLST 135
           PT+VVNGAEANR  LSNEF LVVSSWPSSSVQLMG  CIM K+GEKHR +RG++A +LS 
Sbjct: 94  PTIVVNGAEANRLILSNEFSLVVSSWPSSSVQLMGMNCIMAKQGEKHRVLRGIVANSLSY 153

Query: 136 SSLDVMMPKLCNTIQVYLDTKWVGCDTIGLFHSAKVLTFTIVFECLLGIQVEPRVLTMFE 195
             L+ ++PKLC+T++ + +T+W G + I L+ SAKVLTFT+VFECL GI+VE  +L +FE
Sbjct: 154 IGLESLIPKLCDTVKFHHETEWRGKEEISLYRSAKVLTFTVVFECLYGIKVEIGMLEVFE 213

Query: 196 RVLEGVFAPPFRFPGTRFSRAKKARLEIETTLREVVREKRRKIEGKLEEEECGSLLSRMV 255
           RVLEGVFA P  FP ++F+RAKKARLEIET L   VREKRR++E +  E+   +L SR+V
Sbjct: 214 RVLEGVFALPVEFPCSKFARAKKARLEIETFLVGKVREKRREMEKEGAEKPNTTLFSRLV 273

Query: 256 RAMIGGEISEMEVIDNIVLLVFAAHDTTSFAIAMTFKMLSQHTNCYTLLLQEHTDIIRSK 315
             +I G I+E EV+DN+VLLVFAAHDTTS+A++MTFKML+QH  C   LLQEH  I  +K
Sbjct: 274 EELIKGVITEEEVVDNMVLLVFAAHDTTSYAMSMTFKMLAQHPTCRDTLLQEHAQIKANK 333

Query: 316 GPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIEYESFLIPKGWKVLWTTY 375
           G G+ LT+ED+KKMKY+WQV RE++RL PPIFGSFRKA++DI+Y  + IPKGWK+LWTTY
Sbjct: 334 GEGEYLTVEDVKKMKYSWQVVRETMRLSPPIFGSFRKAVADIDYGGYTIPKGWKILWTTY 393

Query: 376 GTHYGEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNILIFVHSVVTRY 435
           GTHY  E F+DP+SF P+RF++P+  Y Y+PFGGGPR CAG+QLAK++IL+F+H VVT +
Sbjct: 394 GTHYNPEIFQDPMSFDPTRFDKPIQAYTYLPFGGGPRLCAGHQLAKISILVFMHFVVTGF 453

Query: 436 DWSLVHPDEPIIMDPLPVPYHGMPIKISPK 466
           DWSLV+PDE I MDPLP P  GMPIKISPK
Sbjct: 454 DWSLVYPDETISMDPLPFPSLGMPIKISPK 483

[Close]

BLAST of CsGy6G022100 vs. TAIR 10
Match: AT5G36110.1 (cytochrome P450, family 716, subfamily A, polypeptide 1 )

HSP 1 Score: 304.3 bits (778), Expect = 1.7e-82
Identity = 181/479 (37.79%), Postives = 272/479 (56.78%), Query Frame = 0


Query: 1   MLSFLAAILLIISIAFVLR---STKRSRNVPPGAMGLPWIGETMVFYRAQSKNRLFEDFV 60
           ++ FL++ILL  S+  +LR   S     N+PPG  GLP IGE+  F  A  +    E F+
Sbjct: 7   IILFLSSILL--SLLLLLRKHLSHFSYPNLPPGNTGLPLIGESFSFLSAGRQGHP-EKFI 66

Query: 61  EPRVAKYGK----IFKTNLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSSSVQLMGEEC 120
             RV ++      +FKT+L GSPT VV GA  N+F  +NE KLVVS WP  SV  +    
Sbjct: 67  TDRVRRFSSSSSCVFKTHLFGSPTAVVTGASGNKFLFTNENKLVVSWWP-DSVNKIFPSS 126

Query: 121 IMQKEGEKHRCVRGLIAATLSTSSLDVMMPKLCNTIQVYLDTKWVGCDTIGLFHSAKVLT 180
           +     E+ R +R L++  +   +L   +  +    Q + +T+W   D + +F   K  T
Sbjct: 127 MQTSSKEEARKLRMLLSQFMKPEALRRYVGVMDEIAQRHFETEWANQDQVIVFPLTKKFT 186

Query: 181 FTIVFECLLGIQVEPRVLTM---FERVLEGVFAPPFRFPGTRFSRAKKARLEIETTLREV 240
           F+I     L ++   RV  +   F  V  G+F+ P   PGTRF+RA KA   +   +  +
Sbjct: 187 FSIACRSFLSMEDPARVRQLEEQFNTVAVGIFSIPIDLPGTRFNRAIKASRLLRKEVSAI 246

Query: 241 VREKRRKIE-GKLEEEECGSLLSRMVRAMIGGEISEMEVIDNIVLLVFAAHDTTSFAIAM 300
           VR+++ +++ GK  EE    +LS M+  M  GE  + ++ D I+ L+   HDT S     
Sbjct: 247 VRQRKEELKAGKALEEH--DILSHML--MNIGETKDEDLADKIIGLLIGGHDTASIVCTF 306

Query: 301 TFKMLSQHTNCYTLLLQEHTDIIRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGS 360
               L++  + Y  +LQE  +I++ K   + L  EDI+KM+Y+W VA E +R+ PP+ G+
Sbjct: 307 VVNYLAEFPHVYQRVLQEQKEILKEKKEKEGLRWEDIEKMRYSWNVACEVMRIVPPLSGT 366

Query: 361 FRKAISDIEYESFLIPKGWKVLWTTYGTHYGEEYFEDPLSFKPSRFE-EPVSQYVYVPFG 420
           FR+AI    ++ F IPKGWK+ W+   TH   +YF +P  F+P+RFE      Y YVPFG
Sbjct: 367 FREAIDHFSFKGFYIPKGWKLYWSATATHMNPDYFPEPERFEPNRFEGSGPKPYTYVPFG 426

Query: 421 GGPRACAGYQLAKLNILIFVHSVVTRYDWSLVHPDE-PIIMDPLPVPYHGMPIKISPKS 467
           GGPR C G + A+L ILIF+H++V R+ W  V P+E  I++DPLP+P  G+PI+I P+S
Sbjct: 427 GGPRMCPGKEYARLEILIFMHNLVNRFKWEKVFPNENKIVVDPLPIPDKGLPIRIFPQS 477

[Close]

BLAST of CsGy6G022100 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 255.4 bits (651), Expect = 9.2e-68
Identity = 151/467 (32.33%), Postives = 250/467 (53.53%), Query Frame = 0


Query: 5   LAAILLIISIAFVLRSTKRSRNVPPGAMGLPWIGETMVFYRAQSKNRLFEDFVEPRVAKY 64
           +  +++++   + L   ++   +PPG+MGLP+IGET+  Y     +     F   R  KY
Sbjct: 27  IVVVVVVLLFKWWLHWKEQRLRLPPGSMGLPYIGETLRLYTENPNS-----FFATRQNKY 86

Query: 65  GKIFKTNLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRC 124
           G IFKT+++G P V+++  EA R  L ++  L   ++P S  +++G E +   +G  H  
Sbjct: 87  GDIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPYHST 146

Query: 125 VRGLIAATLSTSSLDVMMPKLCNTIQVYLDT--KWVGCDTIGLFHSAKVLTFTIVFECLL 184
           ++ L+ ++   S+L    P + +   + L T   W    +I      K   F +      
Sbjct: 147 LKRLVQSSFMPSAL---RPTVSHIELLVLQTLSSWTSQKSINTLEYMKRYAFDVAIMSAF 206

Query: 185 GIQVEPRVL----TMFERVLEGVFAPPFRFPGTRFSRAKKARLEIETTLREVVREKRRKI 244
           G + EP  +     +++R+  G  + P   PGT F ++ KAR+E+   LR+V+ EKRR+ 
Sbjct: 207 GDKEEPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELSEELRKVI-EKRRE- 266

Query: 245 EGKLEEEECGSLLSRMVRAMIGGEISEMEVIDNIVLLVFAAHDTTSFAIAMTFKMLSQHT 304
            G+ E    G LL    +   G  +S+ ++ DNI+ ++FAA DTT+  +    K L  H 
Sbjct: 267 NGREEGGLLGVLLGAKDQKRNG--LSDSQIADNIIGVIFAATDTTASVLTWLLKYLHDHP 326

Query: 305 NCYTLLLQEHTDIIRS-KGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDI 364
           N    + +E   I +  K   + ++ ED +KM  T +V +E++R    +  +FR+A+ D+
Sbjct: 327 NLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAASVLSFTFREAVQDV 386

Query: 365 EYESFLIPKGWKVLWTTYGTHYGEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGY 424
           EY+ +LIPKGWKVL      H+  E+F DP  F PSRFE     Y Y+PFG G  +C G 
Sbjct: 387 EYDGYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFEVAPKPYTYMPFGNGVHSCPGS 446

Query: 425 QLAKLNILIFVHSVVTRYDWSLVHPDEPIIMDPLPVPYHGMPIKISP 465
           +LAKL +LI +H + T + W ++  +E I   P PVP  G+PI+++P
Sbjct: 447 ELAKLEMLILLHHLTTSFRWEVIGDEEGIQYGPFPVPKKGLPIRVTP 481

[Close]

BLAST of CsGy6G022100 vs. TAIR 10
Match: AT2G29090.2 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 255.4 bits (651), Expect = 9.2e-68
Identity = 151/467 (32.33%), Postives = 250/467 (53.53%), Query Frame = 0


Query: 5   LAAILLIISIAFVLRSTKRSRNVPPGAMGLPWIGETMVFYRAQSKNRLFEDFVEPRVAKY 64
           +  +++++   + L   ++   +PPG+MGLP+IGET+  Y     +     F   R  KY
Sbjct: 27  IVVVVVVLLFKWWLHWKEQRLRLPPGSMGLPYIGETLRLYTENPNS-----FFATRQNKY 86

Query: 65  GKIFKTNLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRC 124
           G IFKT+++G P V+++  EA R  L ++  L   ++P S  +++G E +   +G  H  
Sbjct: 87  GDIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPYHST 146

Query: 125 VRGLIAATLSTSSLDVMMPKLCNTIQVYLDT--KWVGCDTIGLFHSAKVLTFTIVFECLL 184
           ++ L+ ++   S+L    P + +   + L T   W    +I      K   F +      
Sbjct: 147 LKRLVQSSFMPSAL---RPTVSHIELLVLQTLSSWTSQKSINTLEYMKRYAFDVAIMSAF 206

Query: 185 GIQVEPRVL----TMFERVLEGVFAPPFRFPGTRFSRAKKARLEIETTLREVVREKRRKI 244
           G + EP  +     +++R+  G  + P   PGT F ++ KAR+E+   LR+V+ EKRR+ 
Sbjct: 207 GDKEEPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELSEELRKVI-EKRRE- 266

Query: 245 EGKLEEEECGSLLSRMVRAMIGGEISEMEVIDNIVLLVFAAHDTTSFAIAMTFKMLSQHT 304
            G+ E    G LL    +   G  +S+ ++ DNI+ ++FAA DTT+  +    K L  H 
Sbjct: 267 NGREEGGLLGVLLGAKDQKRNG--LSDSQIADNIIGVIFAATDTTASVLTWLLKYLHDHP 326

Query: 305 NCYTLLLQEHTDIIRS-KGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDI 364
           N    + +E   I +  K   + ++ ED +KM  T +V +E++R    +  +FR+A+ D+
Sbjct: 327 NLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAASVLSFTFREAVQDV 386

Query: 365 EYESFLIPKGWKVLWTTYGTHYGEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGY 424
           EY+ +LIPKGWKVL      H+  E+F DP  F PSRFE     Y Y+PFG G  +C G 
Sbjct: 387 EYDGYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFEVAPKPYTYMPFGNGVHSCPGS 446

Query: 425 QLAKLNILIFVHSVVTRYDWSLVHPDEPIIMDPLPVPYHGMPIKISP 465
           +LAKL +LI +H + T + W ++  +E I   P PVP  G+PI+++P
Sbjct: 447 ELAKLEMLILLHHLTTSFRWEVIGDEEGIQYGPFPVPKKGLPIRVTP 481

[Close]

BLAST of CsGy6G022100 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 248.1 bits (632), Expect = 1.5e-65
Identity = 144/461 (31.24%), Postives = 244/461 (52.93%), Query Frame = 0


Query: 8   ILLIISIAFVLRSTKRSRNVPPGAMGLPWIGETMVFYRAQSKNRLFEDFVEPRVAKYGKI 67
           + L+  IA V RS+     +PPG MG P++GET   Y +Q  N  F      +  +YG +
Sbjct: 17  LCLLRFIAGVRRSSSTKLPLPPGTMGYPYVGETFQLY-SQDPNVFF----AAKQRRYGSV 76

Query: 68  FKTNLMGSPTVVVNGAEANRFFLSNEFKLVVSSWPSSSVQLMGEECIMQKEGEKHRCVRG 127
           FKT+++G P V+++  EA +F L  +  L   ++P+S  +++G++ I   +G+ H  +R 
Sbjct: 77  FKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHSKLRK 136

Query: 128 LIAATLSTSSLDVMMPKLCNTIQVYLDTKWVGCDTIGLFHSAKVLTFTIVFECLLG---I 187
           L+       ++  M+P + +  Q  L++ W G   +  +   K  TF +    +LG   +
Sbjct: 137 LVLRAFMPDAIRNMVPHIESIAQESLNS-WDGTQ-LNTYQEMKTYTFNVALISILGKDEV 196

Query: 188 QVEPRVLTMFERVLEGVFAPPFRFPGTRFSRAKKARLEIETTLREVVREKRRKIEGKLEE 247
                +   +  + +G  + P   PGT F +A KAR E+   L  ++ ++R+      + 
Sbjct: 197 YYREDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANILSKRRQNPSSHTD- 256

Query: 248 EECGSLLSRMVRAMIGGEISEMEVIDNIVLLVFAAHDTTSFAIAMTFKMLSQHTNCYTLL 307
                LL   +    G  +++ ++ DNI+ ++FAA DTT+  +    K L+ +      +
Sbjct: 257 -----LLGSFMEDKAG--LTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPTVLEAV 316

Query: 308 LQEHTDIIRSKGPGQNLTMEDIKKMKYTWQVARESIRLFPPIFGSFRKAISDIEYESFLI 367
            +E   I + K  G++LT ED KKM  T++V +E++R    +  +FR+A+ D+EYE +LI
Sbjct: 317 TEEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDVEYEGYLI 376

Query: 368 PKGWKVLWTTYGTHYGEEYFEDPLSFKPSRFEEPVSQYVYVPFGGGPRACAGYQLAKLNI 427
           PKGWKVL      H+  + F DP  F PSRFE       ++PFG G  +C G +LAKL I
Sbjct: 377 PKGWKVLPLFRNIHHNADIFSDPGKFDPSRFEVAPKPNTFMPFGSGIHSCPGNELAKLEI 436

Query: 428 LIFVHSVVTRYDWSLVHPDEPIIMDPLPVPYHGMPIKISPK 466
            + +H + T+Y WS+V P + I   P  +P +G+PI +  K
Sbjct: 437 SVLIHHLTTKYRWSIVGPSDGIQYGPFALPQNGLPIALERK 462

The following BLAST results are available for this feature:

BLAST of CsGy6G022100 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6WG30	1.5e-94	39.58	Taxadiene 5-alpha hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=2	[more]
Q8W4T9	4.9e-90	37.23	Taxane 13-alpha-hydroxylase OS=Taxus cuspidata OX=99806 GN=CYP725A2 PE=1 SV=1	[more]
Q50EK0	5.4e-89	38.62	Cytochrome P450 716B2 OS=Picea sitchensis OX=3332 GN=CYP716B2 PE=2 SV=1	[more]
Q50EK1	2.1e-88	38.32	Cytochrome P450 716B1 OS=Picea sitchensis OX=3332 GN=CYP716B1 PE=2 SV=1	[more]
Q84KI1	2.3e-87	38.36	Taxoid 14-beta-hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=1	[more]

BLAST of CsGy6G022100 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004140249.1	0.0	100.00	taxadiene 5-alpha hydroxylase [Cucumis sativus] >KAE8647348.1 hypothetical prote...	[more]
XP_008449518.1	0.0	94.75	PREDICTED: taxadiene 5-alpha hydroxylase [Cucumis melo]	[more]
XP_038888983.1	9.85e-304	88.87	taxadiene 5-alpha hydroxylase [Benincasa hispida]	[more]
KAG7012036.1	1.60e-291	84.75	hypothetical protein SDJN02_26944, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_023554746.1	6.52e-291	84.75	taxadiene 5-alpha hydroxylase isoform X2 [Cucurbita pepo subsp. pepo]	[more]

BLAST of CsGy6G022100 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BLK9	0.0	94.75	taxadiene 5-alpha hydroxylase OS=Cucumis melo OX=3656 GN=LOC103491382 PE=3 SV=1	[more]
A0A0A0KEB2	0.0	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G423370 PE=3 SV=1	[more]
A0A6J1GJY9	3.68e-290	84.53	taxadiene 5-alpha hydroxylase isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1HZM9	6.41e-288	83.69	taxadiene 5-alpha hydroxylase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11146...	[more]
A0A6J1GK43	3.03e-286	84.90	taxadiene 5-alpha hydroxylase isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]

BLAST of CsGy6G022100 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G42850.1	8.1e-173	63.78	cytochrome P450, family 718	[more]
AT5G36110.1	1.7e-82	37.79	cytochrome P450, family 716, subfamily A, polypeptide 1	[more]
AT2G29090.1	9.2e-68	32.33	cytochrome P450, family 707, subfamily A, polypeptide 2	[more]
AT2G29090.2	9.2e-68	32.33	cytochrome P450, family 707, subfamily A, polypeptide 2	[more]
AT5G45340.1	1.5e-65	31.24	cytochrome P450, family 707, subfamily A, polypeptide 3	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 216..236
None	No IPR available	PIRSR	PIRSR000047-1	PIRSR000047-1	coord: 5..446 e-value: 2.3E-12 score: 44.4
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 16..464
None	No IPR available	PANTHER	PTHR24286:SF221	TAXADIENE 5-ALPHA HYDROXYLASE	coord: 16..464
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 405..414 score: 58.89 coord: 414..425 score: 33.52 coord: 334..345 score: 32.64 coord: 278..295 score: 28.61
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 28..449 e-value: 7.7E-56 score: 189.7
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 373..397 score: 26.96 coord: 267..284 score: 26.61 coord: 414..437 score: 22.92 coord: 61..80 score: 30.76 coord: 404..414 score: 50.41
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 17..465 e-value: 2.9E-109 score: 367.8
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 22..464
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 407..416

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsGy6G022100.1	CsGy6G022100.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen