CsGy6G018160 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy6G018160
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionMetallocarboxypeptidase A-like protein
LocationGy14Chr6: 18938409 .. 18943181 (+)
RNA-Seq ExpressionCsGy6G018160
SyntenyCsGy6G018160
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTCGCCCTTTCTCTTCTTTTTTCCCTTCCTTATGGGTCACCTTCTTCTTCTTCTTCTTCACATCTTTCATGTGTCTTTTCGTTACTGCTGATGATCCCAATAGATTAACTCCAATCAACCGCGATCTTTATCATTCTAGGTGGGTAATTCTCTGATTCGGATTCCTTTACTTCTTTTTGTTAATTACGATGGATGAGATTAAGTGCGAAGTATAATTTGCTGGTTACTATGCTAATGGTTGTTGTTCTCCTTCTTCTCTCTTTGGAATTTGGGTATTTAATCATATAACTGATGCACATTCTCTGTTGTAATTTTGGTTTTAGAAATTTGATGTAGTGGCTAGATATGTGTGTATTTAACTTTCCCAATTCTGGAAAAAGGGTAAGTTTGATCTTATGGCCATATTCTGTGCGCCTCTGTTTTATAGAGTTTGATACAACTCGATCCAAGTTTTTTTTTTTGGAGATGTTCACAGTAGGGTCTTTATCTTTTTGGATATGAACTTTTGGAATCATTTGGTTTGAATACTTTCAATAGCTGAAACTTCCCATTTTGTTGGGTGATTGGATATTGCAGTGTTGATTTGATGAAGGAGATAGAGGCTTTGGTCCATCGACACTCAGATAAGCTGACTGTGTGTTCTTGTTGTTGGTTCTTCTTTATTTAGGCTTGATGCTTTTGGTTTGGTTACTTATTTTCAATATTTAAGCAGATAGAGACAATGAAGTCCAAAAATAAGGGCTATGCTGCAGAGATTCCAGTTGTTACTTATCGCCATGGAAGGAATAACATTGATGACACCTCAAAATTCCGGATACTTCTTGTAAGTTTTGTGTTTAATTGTGTACTCATTTGGTCTTCTAGGCTTTTATTCCATTGATATTATTTTTAGACTGATGCTTTATTCATGTTGAAAGTGAAAGCTGCAAGGCTTCTTGATCTAAACAAAAATGGGATTGATTTAGATTTTAATTTTTTTTTCTGTCTTTCTTTCTTTTCTTTTCCTCTCCTTAACGACTAAGGAACATATCATCTCAGATTGGCAATTTGCTGGTGTTCTTGACAGATGATTACTCCTTGTCTTCCAGAGTTTTGGACAGCATGGAAGGGAGCTCATAACAACAGAAGTTGCACTAAGAATTCTCTTAATTTTGAGTGAAGAACATTTGTTGCCCCACATGGATCGAGCTTCCTTAAACAACACACTCGCTAAGCTTGTCATTAAGGTATGCGTGGAGGCTGCAACTTTTTATATTGATAAGTGGAATACATTTCATCATCTTCTTCTCCACCTACTAGGTTTCGAACCCATAACCTAAGAGTTGGACCAAATTTTAAGCCAAACTCTTAACCATTAAGCTACGAAACAGTTGGGTTTGATAAGTGTAATAAGTTATAATGGAGTAAAAATCATATTGTTCTATAACTTTAGGAAACTTGCAGATAGTCGCACGGGAATCATGGCTAATTGAACAAACGATCAAAAGGATTAGTGGGATGCTTCAACTTTCCTCTTCCACCTAGCCCCTTCCTTTCTTTTTATGTAGCAATGAAGTTCGTGCAGTTAACCCTATAAAGCCCAGTTTTTGCTGTTCTTTTTTTTTTTCAAACTAAAGACAAACTTGTCTCTTTTCGTTGAATAAATGAAAAAGATTAATGCTAAGTGAAGGAAACTAACAAAGGGAGACTTAAAAGCTTGTAAAACAAATCTATGCTTTTAACTGATATTGGTGCTTCGATTTTAAATCACAAGATATTGTATGCATAAATAATGTGCTTGCTTATGTATGTTGTCAGGTGGTGCCAATGGAAAACTTAAATGGGCGAAGGCTTGTCGAGGGTGGGGATTTATGTGAAAGAAGAAATGGTAATGCAGAAGATTTAATCCATTATATAGTCACGGAAAGTCTCCAAGGTAGATATTGACATACCTGCGTTTGTCTTCTATGGCAGGGAGAGGTGTTGATTTGAACAGGAATTGGAGTGTCGATTGGGGTAAAAAGGAAAAGGTTGCTATCTATCTTCTGATTTTCTTTGAAATAATTTGACAAGGTTTTGATATAGTTTGTTAACTAAGTTATAAGTTCTTATTGGAAGGCAAGGAGAAACCATCATATTCATTTCTTGAAACAAGGCCTTTTCTTATGAAGCTCTATGGATGTTTGTCTGGCTTTTGTGAATGATAGTTGTTGTGACACTCTCTTCTATTCCAAGCATTTTAGTCATTCTTCTCCCTATTACAGGATTATGATCCTTATGAGGAAAATCCCGGATTTGCACCTTTCAGCGAACCCGAAACTCAAATTATGCGAAAACTTGCCTTGAGATTTGATCCACATATATGGGTCAATGTGCACTCTGGAATGGAGGTAAGAAACAATACCAAGATTTAGTTATTAACCTGGATTATGATAGGCTTCATTATCTGGTCAGTGCGGATTTGATCTTTTCCCATATGACTCGTTCCCTTGAAGCAAAATTGATCGTGCTACTTTTTCACAATCTCTCCCTTTCCTTTCAATCTTATGGAGCTTGGGAGAACACTGGAGTAGCTACATCTAACTATTTTCTTTTAAACCTGGATTATGATTAGTGAAAATTTTAATGAATACAGACTACAGTTGCTAGTCACCATTATTAAAACCCAGAATAAATTGTGTCTTCTTTTTACTTCCTTCTCTGTTAAAATCACAGCTTAACAATGTGATTGGTTCAGTTGACTGATGAAACTGTACTTAATATTCCACGACATAAATATGTTTAAGTTACAAGAGCATTCAAGCTTGTGTCTAAAAGATATCTGAACATTGAAAATGTTTAACAAGTATCCAATATCTCAGTTATGTTTAATAGATCCCTGACATTTTCGGCTCTCTCCCTCTTTCGAATTTGTTATTACTACTGGATACAGATTTCTGAATCCTGTTTATGAAAAAGAAAATCATAACAAAATCGTATTACTGTTATTTATTAACACCTTTTTTTTTTTCTCTCTGCTCTGTTTATTTGCTGAACTAAAAGATGAGGTTTTCCCATGCTCATTTTATACGACTTGCTAGTTTTGATTGTTACTTCTTTAGGCTTTATTCATGCCTTATGACCACAAAAACATAACTCCTGACGGTGAAATTTCTCAACAAATGAAACTTCTGCTTGAAGAGCTGAATATTCTCCATTGCCACAGCCGCTGCATGATAGGATCAGGCGGAGGTTCGGTCGGGTTAGTTCACTTCTTCTGACTACAATATGATAGCAGTTTACCATTTATACACCTGTTTTATATGTTTTTAAATGAATCTTCATTTGTCATTCTTTGATATTTGTTATAATATAGACAAATGAGTAACGTTTTGGTGCAACTCGTACTACACCAAAGTATTTTTCTGGACAAATATTGGACAATTTATTTGATTAGCCCACCTTCTTAGGTACCTACCAGTTGCATTGTGTCAATGTAGGAAAATTTTCGAATCACAGCTAGAAGTTCAGGAATGCAGGGAGGACTTCTAAAGTTTTAGGGAAAAATAATTTCTGCTCTAAACGTTCAGTTTATGAATTACAACTCTAAATGAATAGTTATATCAAGTTGATCAATTTAAACCTTAATAACTATGCTCACTCTATTTTACTTAAATTCAACCAAAAATCATCTGAAACTCTTCTTCAAAACTTTCGTATCATGTAATTATTCAAAATAAGTAGAGACAAATAGAAAACTACAAGAAGATGAAAATACTGCCACTATCCAGTTTATTCTAGTATTTCTCTCCAATATTATGTTCATTTTTAGATATAAAAAACCTTAACGTCTTACATAAATGTAAACATTATACATTAACAATTTTAATATGAAATCCTTTCGAGTTTTAATTGATAAAATTATTAATATAAATTGATTTTTCACTGAAAGTTGAAAACTAACTAAACAGCTAATCAAAATTCAAGGTTAAATCTAAAACTTATAGCATTTCTTTTCCAATGCATCAAAACTTTTAGATGCCTCCTTGCTTAATCTGAAGGCTAATTGATTTGAATTATTTTTTCTTTTAATTGAATCCTTTAATTGTTCTTGCTATACCATCCCTGATGTGCAATTGGGTCTGAATTTCTTCCTGCATAATGATTGTTGTCTTAAGGTATCTGGCACATGGGACAGCCACAGATTTCATGTTTGACAAGGCTCGAGTTCCTATGGCTTTCACATTTGAGGTTTGTCAAACAAGTTCATAAAATAATCTTAGTTTAATTTTTGGTCTTAAAATAAGTCTCTCTTCTTCGTGTGATTATATCAGATATATGGAGACGAAGCTGCCACATCAAAAGACTGCTTTAGAATGTTCAATCCCACAGATCCCAATACCTTCGACGTATGCAACCATCTTTTCTTGTCATATTGGCCAATTGATGCCCATGGAAATCCTTAGGCTGAATTCGTTTTTCATCTTTGTTTTCAGAGGGTGCTTGGCGATTGGTCTGCTGCGTTTTTTACAATATTTAAGATGGGTCCTGAGTATCTCGACGAAACTGAGTTCCGTCCAAAAAGTAACATAGACAAGCTGGTGTCCATTGATGAATATCTTGAAGGTTACTTGATTGAGAGAAGTAGCAGATATGGAAAGAAGAGGGAGGTGTTTGACCTAGGGATGCAAGAGATGAGAACTTATTTTAGGCTATTTTTGTTGTCATCAGTTCTATTGATGTTCATGTTTTGTTCTAGAATTTCTAAAAACAAGTTCACCAGACCACTCGTTTCTGCCATTTCCATTTGA

mRNA sequence

ATGGGTCGCCCTTTCTCTTCTTTTTTCCCTTCCTTATGGGTCACCTTCTTCTTCTTCTTCTTCACATCTTTCATGTGTCTTTTCGTTACTGCTGATGATCCCAATAGATTAACTCCAATCAACCGCGATCTTTATCATTCTAGTGTTGATTTGATGAAGGAGATAGAGGCTTTGGTCCATCGACACTCAGATAAGCTGACTATAGAGACAATGAAGTCCAAAAATAAGGGCTATGCTGCAGAGATTCCAGTTGTTACTTATCGCCATGGAAGGAATAACATTGATGACACCTCAAAATTCCGGATACTTCTTAGTTTTGGACAGCATGGAAGGGAGCTCATAACAACAGAAGTTGCACTAAGAATTCTCTTAATTTTGAGTGAAGAACATTTGTTGCCCCACATGGATCGAGCTTCCTTAAACAACACACTCGCTAAGCTTGTCATTAAGGTGGTGCCAATGGAAAACTTAAATGGGCGAAGGCTTGTCGAGGGTGGGGATTTATGTGAAAGAAGAAATGGGAGAGGTGTTGATTTGAACAGGAATTGGAGTGTCGATTGGGGTAAAAAGGAAAAGGATTATGATCCTTATGAGGAAAATCCCGGATTTGCACCTTTCAGCGAACCCGAAACTCAAATTATGCGAAAACTTGCCTTGAGATTTGATCCACATATATGGGTCAATGTGCACTCTGGAATGGAGGCTTTATTCATGCCTTATGACCACAAAAACATAACTCCTGACGGTGAAATTTCTCAACAAATGAAACTTCTGCTTGAAGAGCTGAATATTCTCCATTGCCACAGCCGCTGCATGATAGGATCAGGCGGAGGTTCGGTCGGGTATCTGGCACATGGGACAGCCACAGATTTCATGTTTGACAAGGCTCGAGTTCCTATGGCTTTCACATTTGAGATATATGGAGACGAAGCTGCCACATCAAAAGACTGCTTTAGAATGTTCAATCCCACAGATCCCAATACCTTCGACAGGGTGCTTGGCGATTGGTCTGCTGCGTTTTTTACAATATTTAAGATGGGTCCTGAGTATCTCGACGAAACTGAGTTCCGTCCAAAAAGTAACATAGACAAGCTGGTGTCCATTGATGAATATCTTGAAGGTTACTTGATTGAGAGAAGTAGCAGATATGGAAAGAAGAGGGAGGTGTTTGACCTAGGGATGCAAGAGATGAGAACTTATTTTAGGCTATTTTTGTTGTCATCAGTTCTATTGATGTTCATGTTTTGTTCTAGAATTTCTAAAAACAAGTTCACCAGACCACTCGTTTCTGCCATTTCCATTTGA

Coding sequence (CDS)

ATGGGTCGCCCTTTCTCTTCTTTTTTCCCTTCCTTATGGGTCACCTTCTTCTTCTTCTTCTTCACATCTTTCATGTGTCTTTTCGTTACTGCTGATGATCCCAATAGATTAACTCCAATCAACCGCGATCTTTATCATTCTAGTGTTGATTTGATGAAGGAGATAGAGGCTTTGGTCCATCGACACTCAGATAAGCTGACTATAGAGACAATGAAGTCCAAAAATAAGGGCTATGCTGCAGAGATTCCAGTTGTTACTTATCGCCATGGAAGGAATAACATTGATGACACCTCAAAATTCCGGATACTTCTTAGTTTTGGACAGCATGGAAGGGAGCTCATAACAACAGAAGTTGCACTAAGAATTCTCTTAATTTTGAGTGAAGAACATTTGTTGCCCCACATGGATCGAGCTTCCTTAAACAACACACTCGCTAAGCTTGTCATTAAGGTGGTGCCAATGGAAAACTTAAATGGGCGAAGGCTTGTCGAGGGTGGGGATTTATGTGAAAGAAGAAATGGGAGAGGTGTTGATTTGAACAGGAATTGGAGTGTCGATTGGGGTAAAAAGGAAAAGGATTATGATCCTTATGAGGAAAATCCCGGATTTGCACCTTTCAGCGAACCCGAAACTCAAATTATGCGAAAACTTGCCTTGAGATTTGATCCACATATATGGGTCAATGTGCACTCTGGAATGGAGGCTTTATTCATGCCTTATGACCACAAAAACATAACTCCTGACGGTGAAATTTCTCAACAAATGAAACTTCTGCTTGAAGAGCTGAATATTCTCCATTGCCACAGCCGCTGCATGATAGGATCAGGCGGAGGTTCGGTCGGGTATCTGGCACATGGGACAGCCACAGATTTCATGTTTGACAAGGCTCGAGTTCCTATGGCTTTCACATTTGAGATATATGGAGACGAAGCTGCCACATCAAAAGACTGCTTTAGAATGTTCAATCCCACAGATCCCAATACCTTCGACAGGGTGCTTGGCGATTGGTCTGCTGCGTTTTTTACAATATTTAAGATGGGTCCTGAGTATCTCGACGAAACTGAGTTCCGTCCAAAAAGTAACATAGACAAGCTGGTGTCCATTGATGAATATCTTGAAGGTTACTTGATTGAGAGAAGTAGCAGATATGGAAAGAAGAGGGAGGTGTTTGACCTAGGGATGCAAGAGATGAGAACTTATTTTAGGCTATTTTTGTTGTCATCAGTTCTATTGATGTTCATGTTTTGTTCTAGAATTTCTAAAAACAAGTTCACCAGACCACTCGTTTCTGCCATTTCCATTTGA

Protein sequence

MGRPFSSFFPSLWVTFFFFFFTSFMCLFVTADDPNRLTPINRDLYHSSVDLMKEIEALVHRHSDKLTIETMKSKNKGYAAEIPVVTYRHGRNNIDDTSKFRILLSFGQHGRELITTEVALRILLILSEEHLLPHMDRASLNNTLAKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNRNWSVDWGKKEKDYDPYEENPGFAPFSEPETQIMRKLALRFDPHIWVNVHSGMEALFMPYDHKNITPDGEISQQMKLLLEELNILHCHSRCMIGSGGGSVGYLAHGTATDFMFDKARVPMAFTFEIYGDEAATSKDCFRMFNPTDPNTFDRVLGDWSAAFFTIFKMGPEYLDETEFRPKSNIDKLVSIDEYLEGYLIERSSRYGKKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSRISKNKFTRPLVSAISI*
Homology
BLAST of CsGy6G018160 vs. ExPASy Swiss-Prot
Match: Q5B011 (Putative metallocarboxypeptidase ecm14 OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=ecm14 PE=3 SV=2)

HSP 1 Score: 57.4 bits (137), Expect = 4.7e-07
Identity = 74/316 (23.42%), Postives = 133/316 (42.09%), Query Frame = 0

Query: 81  EIPVVTYRHGRNNIDDTSKFRILLSFGQHGRELITTEVALRILLILSEEHLLPHMDRASL 140
           +IP +  R GR++    S+  IL+  G H RE I+T     I   L    +  +   A++
Sbjct: 226 QIPAL--RLGRSSQPGESRPMILIVGGNHAREWISTSTVAYIAYNL----MTRYGSSAAV 285

Query: 141 NNTLAKLVIKVVPMENLNGRRLVEGGDLCERRNGR--------GVDLNRNWSVDWGKKEK 200
            + L      +VP  N +G       D   R+N +        G+DL+R+WS +W   + 
Sbjct: 286 TSLLQDYDWVLVPTVNPDGYVYTWDSDRLWRKNRQPTSFRFCPGIDLDRSWSFEWDGNQT 345

Query: 201 DYDPYEEN-PGFAPFSEPETQIMRKLALRFDPHI------WVNVHSGMEALFMPYDH--K 260
             +P  EN  G  PF   E Q + + AL    H       +++ HS  + +  PY +   
Sbjct: 346 QTNPCSENYAGEEPFEGVEAQQLAEWALNETEHNNARFVGFLDFHSYAQQILYPYTYSCS 405

Query: 261 NITPDGEISQQMKLLLEELNILHCH------SRC----MIGSGGGS------VGYLAHGT 320
           ++ P  E  +++ L L ++  L  H      S C    +  SG  S      VG  + G+
Sbjct: 406 SVPPTLESLEELGLGLAKVIRLTTHEIYDVTSACEGVVIPASGDKSAKSLFPVGGSSGGS 465

Query: 321 ATDFMFDKARVPMAFTFEIYGDEAATSKDCFRMFNPTDPNTFDRVLGDWSAAFFTIFKMG 364
             D+ + + R   A+  ++        +  +    P+     + ++   + AF  + K+G
Sbjct: 466 GLDWFYHQLRTRYAYQIKL------RDRGSYGFLLPS-----EYIVPTGTEAFNAVLKLG 523

BLAST of CsGy6G018160 vs. ExPASy Swiss-Prot
Match: Q8N4T0 (Carboxypeptidase A6 OS=Homo sapiens OX=9606 GN=CPA6 PE=1 SV=2)

HSP 1 Score: 56.6 bits (135), Expect = 8.1e-07
Identity = 69/283 (24.38%), Postives = 119/283 (42.05%), Query Frame = 0

Query: 37  LTPINRDLYHSSVDLMKEIEALVHRHSDKLTIETMKSKNKGYAAEIPVVTYRHGRNNIDD 96
           L+  N ++YHS  ++   +  L   HS  + + ++    +G +  I     + GR +   
Sbjct: 131 LSGYNYEVYHSLEEIQNWMHHLNKTHSGLIHMFSIGRSYEGRSLFI----LKLGRRS--- 190

Query: 97  TSKFRILLSFGQHGRELITTEVALRILLILSEEHLLPHMDRASLNNTLAKLVIKVVPMEN 156
             K  + +  G H RE I        +    +E LL +    ++   L  L   ++P+ N
Sbjct: 191 RLKRAVWIDCGIHAREWIGPAFCQWFV----KEALLTYKSDPAMRKMLNHLYFYIMPVFN 250

Query: 157 LNGRRLVEGGDLCER----RNG----RGVDLNRNWSVDWGKKEKDYDPYEEN-PGFAPFS 216
           ++G       D   R    RN     RGVD NRNW V W  +     P ++   G  P S
Sbjct: 251 VDGYHFSWTNDRFWRKTRSRNSRFRCRGVDANRNWKVKWCDEGASMHPCDDTYCGPFPES 310

Query: 217 EPETQIMRKLALRFDPHI--WVNVHSGMEALFMPYDHKNIT-PDGEISQQMKLLLEELNI 276
           EPE + +     +   HI  +++ H+  + L  PY +K  T P+    +      + +N 
Sbjct: 311 EPEVKAVANFLRKHRKHIRAYLSFHAYAQMLLYPYSYKYATIPNFRCVE--SAAYKAVNA 370

Query: 277 LHCHSRCMIGSGGGSVG-YLAHGTATDFMFDKARVPMAFTFEI 307
           L          G  S   Y++ G++ D+ + K  +P AF FE+
Sbjct: 371 LQSVYGVRYRYGPASTTLYVSSGSSMDWAY-KNGIPYAFAFEL 399

BLAST of CsGy6G018160 vs. ExPASy Swiss-Prot
Match: Q5U901 (Carboxypeptidase A6 OS=Mus musculus OX=10090 GN=Cpa6 PE=2 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 4.4e-05
Identity = 64/287 (22.30%), Postives = 113/287 (39.37%), Query Frame = 0

Query: 37  LTPINRDLYHSSVDLMKEIEALVHRHSDKLTIETMKSKNKGYAAEIPVVTYRHGRNNIDD 96
           L+  N ++YHS  D+   +    H +  +  +  + S  + Y    P+   + GR +   
Sbjct: 131 LSEYNYEVYHSLEDIQSWLH---HLNQTQPGLVRVFSIGRSYEGR-PLFIMQLGRKS--R 190

Query: 97  TSKFRILLSFGQHGRELITTEVALRILLILSEEHLLPHMDRASLNNTLAKLVIKVVPMEN 156
             K  + +  G H RE I        +     E +L +    ++   L  L   ++P+ N
Sbjct: 191 AYKRAVWIDCGIHAREWIGPAFCQWFV----REAILTYKTDPAMKKMLNHLYFYIMPVFN 250

Query: 157 LNGRRLVEGGDLCERRNG--------RGVDLNRNWSVDWGKKEKDYDPYEEN-PGFAPFS 216
           ++G       D   R+          RGVD NRNW V W  +     P ++   G  P S
Sbjct: 251 VDGYHFSWTHDRFWRKTRSRDSKFRCRGVDANRNWKVKWCDEGASAHPCDDTYCGPFPES 310

Query: 217 EPETQIMRKLALRFDPHI--WVNVHSGMEALFMPYDHKNIT------PDGEISQQMKLLL 276
           EPE + +     +    I  +++ H+  + L  PY +K  T       +    + +K L 
Sbjct: 311 EPEVKAVANFLRKHRKRIRAYLSFHAYAQMLLYPYSYKYATIPNFSCVEFAAHKAVKALR 370

Query: 277 EELNILHCHSRCMIGSGGGSVGYLAHGTATDFMFDKARVPMAFTFEI 307
               I + H             Y++ G + D+ + K  +P AF FE+
Sbjct: 371 SVHGIRYRH------GPASQTLYVSSGNSMDWAY-KNGIPYAFAFEL 400

BLAST of CsGy6G018160 vs. NCBI nr
Match: XP_004144231.1 (metallocarboxypeptidase A-like protein MCYG_01475 [Cucumis sativus] >KGN47544.1 hypothetical protein Csa_019060 [Cucumis sativus])

HSP 1 Score: 872 bits (2253), Expect = 0.0
Identity = 433/434 (99.77%), Postives = 434/434 (100.00%), Query Frame = 0

Query: 1   MGRPFSSFFPSLWVTFFFFFFTSFMCLFVTADDPNRLTPINRDLYHSSVDLMKEIEALVH 60
           MGRPFSSFFPSLWVTFFFFFFTSFMCLFV+ADDPNRLTPINRDLYHSSVDLMKEIEALVH
Sbjct: 1   MGRPFSSFFPSLWVTFFFFFFTSFMCLFVSADDPNRLTPINRDLYHSSVDLMKEIEALVH 60

Query: 61  RHSDKLTIETMKSKNKGYAAEIPVVTYRHGRNNIDDTSKFRILLSFGQHGRELITTEVAL 120
           RHSDKLTIETMKSKNKGYAAEIPVVTYRHGRNNIDDTSKFRILLSFGQHGRELITTEVAL
Sbjct: 61  RHSDKLTIETMKSKNKGYAAEIPVVTYRHGRNNIDDTSKFRILLSFGQHGRELITTEVAL 120

Query: 121 RILLILSEEHLLPHMDRASLNNTLAKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLN 180
           RILLILSEEHLLPHMDRASLNNTLAKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLN
Sbjct: 121 RILLILSEEHLLPHMDRASLNNTLAKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLN 180

Query: 181 RNWSVDWGKKEKDYDPYEENPGFAPFSEPETQIMRKLALRFDPHIWVNVHSGMEALFMPY 240
           RNWSVDWGKKEKDYDPYEENPGFAPFSEPETQIMRKLALRFDPHIWVNVHSGMEALFMPY
Sbjct: 181 RNWSVDWGKKEKDYDPYEENPGFAPFSEPETQIMRKLALRFDPHIWVNVHSGMEALFMPY 240

Query: 241 DHKNITPDGEISQQMKLLLEELNILHCHSRCMIGSGGGSVGYLAHGTATDFMFDKARVPM 300
           DHKNITPDGEISQQMKLLLEELNILHCHSRCMIGSGGGSVGYLAHGTATDFMFDKARVPM
Sbjct: 241 DHKNITPDGEISQQMKLLLEELNILHCHSRCMIGSGGGSVGYLAHGTATDFMFDKARVPM 300

Query: 301 AFTFEIYGDEAATSKDCFRMFNPTDPNTFDRVLGDWSAAFFTIFKMGPEYLDETEFRPKS 360
           AFTFEIYGDEAATSKDCFRMFNPTDPNTFDRVLGDWSAAFFTIFKMGPEYLDETEFRPKS
Sbjct: 301 AFTFEIYGDEAATSKDCFRMFNPTDPNTFDRVLGDWSAAFFTIFKMGPEYLDETEFRPKS 360

Query: 361 NIDKLVSIDEYLEGYLIERSSRYGKKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSRIS 420
           NIDKLVSIDEYLEGYLIERSSRYGKKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSRIS
Sbjct: 361 NIDKLVSIDEYLEGYLIERSSRYGKKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSRIS 420

Query: 421 KNKFTRPLVSAISI 434
           KNKFTRPLVSAISI
Sbjct: 421 KNKFTRPLVSAISI 434

BLAST of CsGy6G018160 vs. NCBI nr
Match: XP_008464857.1 (PREDICTED: LOW QUALITY PROTEIN: metallocarboxypeptidase A-like protein TRV_02598 [Cucumis melo])

HSP 1 Score: 829 bits (2142), Expect = 2.47e-301
Identity = 413/433 (95.38%), Postives = 420/433 (97.00%), Query Frame = 0

Query: 2   GRPFSSFFPSLWVTFFFFFFTSFMCLFVTADDPNRLTPINRDLYHSSVDLMKEIEALVHR 61
           G PF  FF SLW+TFFFFF TSFMCLFVTADDP+RLTPIN DLYHSSVDLMKEIEALVHR
Sbjct: 76  GSPFLFFFSSLWITFFFFF-TSFMCLFVTADDPDRLTPINHDLYHSSVDLMKEIEALVHR 135

Query: 62  HSDKLTIETMKSKNKGYAAEIPVVTYRHGRNNIDDTSKFRILLSFGQHGRELITTEVALR 121
           H DKLTIETMKSKNKGYAAEIPVVTYR GRNNIDDTSKFRILLSFGQHGRELITTEVALR
Sbjct: 136 HPDKLTIETMKSKNKGYAAEIPVVTYRRGRNNIDDTSKFRILLSFGQHGRELITTEVALR 195

Query: 122 ILLILSEEHLLPHMDRASLNNTLAKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNR 181
           ILLILSEEHLLPHMDRASLNNTL KLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNR
Sbjct: 196 ILLILSEEHLLPHMDRASLNNTLDKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNR 255

Query: 182 NWSVDWGKKEKDYDPYEENPGFAPFSEPETQIMRKLALRFDPHIWVNVHSGMEALFMPYD 241
           NWSVDWGKKEKDYDPYEENPGFAPFSEPETQIMRK+ALRFDPHIWVNVHSGMEALFMPYD
Sbjct: 256 NWSVDWGKKEKDYDPYEENPGFAPFSEPETQIMRKIALRFDPHIWVNVHSGMEALFMPYD 315

Query: 242 HKNITPDGEISQQMKLLLEELNILHCHSRCMIGSGGGSVGYLAHGTATDFMFDKARVPMA 301
           HKN TPDGEI+QQMKLLLEELN LHCHSRCMIGSGGGSVGYLAHGTATDFMFDKARVPMA
Sbjct: 316 HKNTTPDGEIAQQMKLLLEELNHLHCHSRCMIGSGGGSVGYLAHGTATDFMFDKARVPMA 375

Query: 302 FTFEIYGDEAATSKDCFRMFNPTDPNTFDRVLGDWSAAFFTIFKMGPEYLDETEFRPKSN 361
           FTFEIYGDEAA+SKDCFRMFNPTDPNTF+RVL DWSAAFFTIFKMGPEYLDETEF PKSN
Sbjct: 376 FTFEIYGDEAASSKDCFRMFNPTDPNTFNRVLSDWSAAFFTIFKMGPEYLDETEFHPKSN 435

Query: 362 IDKLVSIDEYLEGYLIERSSRYGKKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSRISK 421
           +DKLVSIDEYLEGYLIERSSRYGKKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSRISK
Sbjct: 436 VDKLVSIDEYLEGYLIERSSRYGKKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSRISK 495

Query: 422 NKFTRPLVSAISI 434
           NKFTRPLVSAISI
Sbjct: 496 NKFTRPLVSAISI 507

BLAST of CsGy6G018160 vs. NCBI nr
Match: KAA0038457.1 (metallocarboxypeptidase A-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 800 bits (2067), Expect = 1.71e-291
Identity = 394/410 (96.10%), Postives = 401/410 (97.80%), Query Frame = 0

Query: 25  MCLFVTADDPNRLTPINRDLYHSSVDLMKEIEALVHRHSDKLTIETMKSKNKGYAAEIPV 84
           MCLFVTADDP+RLTPIN DLYHSSVDLMKEIEA+VHRH DKLTIETMKSKNKGYAAEIPV
Sbjct: 1   MCLFVTADDPDRLTPINHDLYHSSVDLMKEIEAMVHRHPDKLTIETMKSKNKGYAAEIPV 60

Query: 85  VTYRHGRNNIDDTSKFRILLSFGQHGRELITTEVALRILLILSEEHLLPHMDRASLNNTL 144
           VTYR GRNNIDDTSKFRILLSFGQHGRELITTEVALRILLILSEEHLLPHMD ASLNNTL
Sbjct: 61  VTYRRGRNNIDDTSKFRILLSFGQHGRELITTEVALRILLILSEEHLLPHMDHASLNNTL 120

Query: 145 AKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNRNWSVDWGKKEKDYDPYEENPGFA 204
            KLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNRNWSVDWGKKEKDYDPYEENPGFA
Sbjct: 121 DKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNRNWSVDWGKKEKDYDPYEENPGFA 180

Query: 205 PFSEPETQIMRKLALRFDPHIWVNVHSGMEALFMPYDHKNITPDGEISQQMKLLLEELNI 264
           PFSEPETQIMRK+ALRFDPHIWVNVHSGMEALFMPYDHKN TPDGEI+QQMKLLLEELN 
Sbjct: 181 PFSEPETQIMRKIALRFDPHIWVNVHSGMEALFMPYDHKNTTPDGEIAQQMKLLLEELNH 240

Query: 265 LHCHSRCMIGSGGGSVGYLAHGTATDFMFDKARVPMAFTFEIYGDEAATSKDCFRMFNPT 324
           LHCHSRCMIGSGGGSVGYLAHGTATDFMFDKARVPMAFTFEIYGDEAA+SKDCFRMFNPT
Sbjct: 241 LHCHSRCMIGSGGGSVGYLAHGTATDFMFDKARVPMAFTFEIYGDEAASSKDCFRMFNPT 300

Query: 325 DPNTFDRVLGDWSAAFFTIFKMGPEYLDETEFRPKSNIDKLVSIDEYLEGYLIERSSRYG 384
           DPNTF+RVL DWSAAFFTIFKMGPEYLDETEF PKSN+DKLVSIDEYLEGYLIERSSRYG
Sbjct: 301 DPNTFNRVLSDWSAAFFTIFKMGPEYLDETEFHPKSNVDKLVSIDEYLEGYLIERSSRYG 360

Query: 385 KKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSRISKNKFTRPLVSAISI 434
           KKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSRISKNKFTRPLVSAISI
Sbjct: 361 KKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSRISKNKFTRPLVSAISI 410

BLAST of CsGy6G018160 vs. NCBI nr
Match: XP_038883968.1 (metallocarboxypeptidase A-like protein MCYG_01475 [Benincasa hispida])

HSP 1 Score: 795 bits (2053), Expect = 6.45e-289
Identity = 396/437 (90.62%), Postives = 411/437 (94.05%), Query Frame = 0

Query: 1   MGRPFSSFFPSLWVTFFFF---FFTSFMCLFVTADDPNRLTPINRDLYHSSVDLMKEIEA 60
           MGRP SSFFPSLW+  FFF   FF SF+CLFVTADDP+RLTPINRDLYHSSVDLMKEI+A
Sbjct: 1   MGRPLSSFFPSLWIRLFFFCFCFFISFICLFVTADDPDRLTPINRDLYHSSVDLMKEIKA 60

Query: 61  LVHRHSDKLTIETMKSKNKGYAAEIPVVTYRHGRNNIDDTSKFRILLSFGQHGRELITTE 120
           LVHRH DKLTIETMKS+NKGY AEIPVVTY HGRNNIDDTSKFRILLSFGQHGRELITTE
Sbjct: 61  LVHRHPDKLTIETMKSRNKGYVAEIPVVTYCHGRNNIDDTSKFRILLSFGQHGRELITTE 120

Query: 121 VALRILLILSEEHLLPHMDRASLNNTLAKLVIKVVPMENLNGRRLVEGGDLCERRNGRGV 180
           VALRIL ILSEE LLPHMDRASLNNTL KLVIKVVPMENLNGRRLVEGGDLCERRNGRGV
Sbjct: 121 VALRILSILSEEQLLPHMDRASLNNTLDKLVIKVVPMENLNGRRLVEGGDLCERRNGRGV 180

Query: 181 DLNRNWSVDWGKKEKDYDPYEENPGFAPFSEPETQIMRKLALRFDPHIWVNVHSGMEALF 240
           DLNRNWSVDWGKKEKDYDPYEENPGFAPFSEPETQIMR LAL FDPH+WVNVHSGMEALF
Sbjct: 181 DLNRNWSVDWGKKEKDYDPYEENPGFAPFSEPETQIMRNLALTFDPHMWVNVHSGMEALF 240

Query: 241 MPYDHKNITPDGEISQQMKLLLEELNILHCHSRCMIGSGGGSVGYLAHGTATDFMFDKAR 300
           MPYDHKN TP+GEI+QQMKLL+EELN LHCH RCMIGSGGGSVGYLAHGTATDFMFDKA 
Sbjct: 241 MPYDHKNTTPEGEIAQQMKLLVEELNNLHCHHRCMIGSGGGSVGYLAHGTATDFMFDKAG 300

Query: 301 VPMAFTFEIYGDEAATSKDCFRMFNPTDPNTFDRVLGDWSAAFFTIFKMGPEYLDETEFR 360
           VPMAFTFEIYGDEAA+SKDCFRMFNPTDP TF+RVLGDWSAAFFTIFK+GPEYL ET+  
Sbjct: 301 VPMAFTFEIYGDEAASSKDCFRMFNPTDPATFNRVLGDWSAAFFTIFKLGPEYLGETKLH 360

Query: 361 PKSNIDKLVSIDEYLEGYLIERSSRYGKKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCS 420
            K N+DKLVSIDEYLEGYLIERSSRYGKKREV DLGMQEMRTYFRLFLLSSVLLMFMFCS
Sbjct: 361 SKGNLDKLVSIDEYLEGYLIERSSRYGKKREVIDLGMQEMRTYFRLFLLSSVLLMFMFCS 420

Query: 421 RISKNKFTRPLVSAISI 434
           RISKNKFTRPLVSAIS+
Sbjct: 421 RISKNKFTRPLVSAISL 437

BLAST of CsGy6G018160 vs. NCBI nr
Match: XP_023002027.1 (metallocarboxypeptidase A-like protein MCYG_01475 [Cucurbita maxima])

HSP 1 Score: 763 bits (1971), Expect = 1.93e-276
Identity = 379/436 (86.93%), Postives = 402/436 (92.20%), Query Frame = 0

Query: 1   MGRPFSSFFPSLWVTFFFFFFTSFMCLFVTADDP--NRLTPINRDLYHSSVDLMKEIEAL 60
           M RP  S   + +  FFFFFFT F C  V A+DP  NRLTPINRDLYHSSVDLMKEIEA+
Sbjct: 1   MARPLRSLCLTFFFFFFFFFFTFFDCFLVAAEDPDNNRLTPINRDLYHSSVDLMKEIEAV 60

Query: 61  VHRHSDKLTIETMKSKNKGYAAEIPVVTYRHGRNNIDDTSKFRILLSFGQHGRELITTEV 120
           VHRHSDKLTIETMKS+NKGY  E+PVVTYRHGRNNIDD SKFRILLSFGQHGRELITTE+
Sbjct: 61  VHRHSDKLTIETMKSRNKGYVVEMPVVTYRHGRNNIDDISKFRILLSFGQHGRELITTEL 120

Query: 121 ALRILLILSEEHLLPHMDRASLNNTLAKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVD 180
           ALRILLILSEE  LPHMDRASLNNTL KLVIKVVPMENLNGRRLVEGGDLCERRNGRGVD
Sbjct: 121 ALRILLILSEELFLPHMDRASLNNTLTKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVD 180

Query: 181 LNRNWSVDWGKKEKDYDPYEENPGFAPFSEPETQIMRKLALRFDPHIWVNVHSGMEALFM 240
           LNRNWSVDWGKKEKDYDPYEENPG +PFSEPETQIMRKLAL FDPHIWVNVHSGMEALFM
Sbjct: 181 LNRNWSVDWGKKEKDYDPYEENPGLSPFSEPETQIMRKLALTFDPHIWVNVHSGMEALFM 240

Query: 241 PYDHKNITPDGEISQQMKLLLEELNILHCHSRCMIGSGGGSVGYLAHGTATDFMFDKARV 300
           PYDHKN TPDGEI+QQMKLLLEELN LHCH RCMIGSGGGSVGYLAHGTATDFMFDKARV
Sbjct: 241 PYDHKNTTPDGEIAQQMKLLLEELNNLHCHHRCMIGSGGGSVGYLAHGTATDFMFDKARV 300

Query: 301 PMAFTFEIYGDEAATSKDCFRMFNPTDPNTFDRVLGDWSAAFFTIFKMGPEYLDETEFRP 360
           PM+FTFEIYGD AA+S DCF+MFNPTDP+TF+RVLGDWSAAFFTIFK+GPEYL ET+   
Sbjct: 301 PMSFTFEIYGDAAASSNDCFKMFNPTDPDTFNRVLGDWSAAFFTIFKLGPEYLGETKLHR 360

Query: 361 KSNIDKLVSIDEYLEGYLIERSSRYGKKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSR 420
           K N+DKLVSIDEYLEGYLIERSSRYGKKREVF+LG+QEMRTYFRLFLLSSVLLMFMFCSR
Sbjct: 361 KGNLDKLVSIDEYLEGYLIERSSRYGKKREVFELGLQEMRTYFRLFLLSSVLLMFMFCSR 420

Query: 421 ISKNKFTRPLVSAISI 434
           ISK+KFT+PLVSA+S+
Sbjct: 421 ISKSKFTKPLVSALSL 436

BLAST of CsGy6G018160 vs. ExPASy TrEMBL
Match: A0A0A0KFG6 (Peptidase_M14 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G358080 PE=3 SV=1)

HSP 1 Score: 872 bits (2253), Expect = 0.0
Identity = 433/434 (99.77%), Postives = 434/434 (100.00%), Query Frame = 0

Query: 1   MGRPFSSFFPSLWVTFFFFFFTSFMCLFVTADDPNRLTPINRDLYHSSVDLMKEIEALVH 60
           MGRPFSSFFPSLWVTFFFFFFTSFMCLFV+ADDPNRLTPINRDLYHSSVDLMKEIEALVH
Sbjct: 1   MGRPFSSFFPSLWVTFFFFFFTSFMCLFVSADDPNRLTPINRDLYHSSVDLMKEIEALVH 60

Query: 61  RHSDKLTIETMKSKNKGYAAEIPVVTYRHGRNNIDDTSKFRILLSFGQHGRELITTEVAL 120
           RHSDKLTIETMKSKNKGYAAEIPVVTYRHGRNNIDDTSKFRILLSFGQHGRELITTEVAL
Sbjct: 61  RHSDKLTIETMKSKNKGYAAEIPVVTYRHGRNNIDDTSKFRILLSFGQHGRELITTEVAL 120

Query: 121 RILLILSEEHLLPHMDRASLNNTLAKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLN 180
           RILLILSEEHLLPHMDRASLNNTLAKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLN
Sbjct: 121 RILLILSEEHLLPHMDRASLNNTLAKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLN 180

Query: 181 RNWSVDWGKKEKDYDPYEENPGFAPFSEPETQIMRKLALRFDPHIWVNVHSGMEALFMPY 240
           RNWSVDWGKKEKDYDPYEENPGFAPFSEPETQIMRKLALRFDPHIWVNVHSGMEALFMPY
Sbjct: 181 RNWSVDWGKKEKDYDPYEENPGFAPFSEPETQIMRKLALRFDPHIWVNVHSGMEALFMPY 240

Query: 241 DHKNITPDGEISQQMKLLLEELNILHCHSRCMIGSGGGSVGYLAHGTATDFMFDKARVPM 300
           DHKNITPDGEISQQMKLLLEELNILHCHSRCMIGSGGGSVGYLAHGTATDFMFDKARVPM
Sbjct: 241 DHKNITPDGEISQQMKLLLEELNILHCHSRCMIGSGGGSVGYLAHGTATDFMFDKARVPM 300

Query: 301 AFTFEIYGDEAATSKDCFRMFNPTDPNTFDRVLGDWSAAFFTIFKMGPEYLDETEFRPKS 360
           AFTFEIYGDEAATSKDCFRMFNPTDPNTFDRVLGDWSAAFFTIFKMGPEYLDETEFRPKS
Sbjct: 301 AFTFEIYGDEAATSKDCFRMFNPTDPNTFDRVLGDWSAAFFTIFKMGPEYLDETEFRPKS 360

Query: 361 NIDKLVSIDEYLEGYLIERSSRYGKKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSRIS 420
           NIDKLVSIDEYLEGYLIERSSRYGKKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSRIS
Sbjct: 361 NIDKLVSIDEYLEGYLIERSSRYGKKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSRIS 420

Query: 421 KNKFTRPLVSAISI 434
           KNKFTRPLVSAISI
Sbjct: 421 KNKFTRPLVSAISI 434

BLAST of CsGy6G018160 vs. ExPASy TrEMBL
Match: A0A1S3CMF4 (LOW QUALITY PROTEIN: metallocarboxypeptidase A-like protein TRV_02598 OS=Cucumis melo OX=3656 GN=LOC103502627 PE=3 SV=1)

HSP 1 Score: 829 bits (2142), Expect = 1.20e-301
Identity = 413/433 (95.38%), Postives = 420/433 (97.00%), Query Frame = 0

Query: 2   GRPFSSFFPSLWVTFFFFFFTSFMCLFVTADDPNRLTPINRDLYHSSVDLMKEIEALVHR 61
           G PF  FF SLW+TFFFFF TSFMCLFVTADDP+RLTPIN DLYHSSVDLMKEIEALVHR
Sbjct: 76  GSPFLFFFSSLWITFFFFF-TSFMCLFVTADDPDRLTPINHDLYHSSVDLMKEIEALVHR 135

Query: 62  HSDKLTIETMKSKNKGYAAEIPVVTYRHGRNNIDDTSKFRILLSFGQHGRELITTEVALR 121
           H DKLTIETMKSKNKGYAAEIPVVTYR GRNNIDDTSKFRILLSFGQHGRELITTEVALR
Sbjct: 136 HPDKLTIETMKSKNKGYAAEIPVVTYRRGRNNIDDTSKFRILLSFGQHGRELITTEVALR 195

Query: 122 ILLILSEEHLLPHMDRASLNNTLAKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNR 181
           ILLILSEEHLLPHMDRASLNNTL KLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNR
Sbjct: 196 ILLILSEEHLLPHMDRASLNNTLDKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNR 255

Query: 182 NWSVDWGKKEKDYDPYEENPGFAPFSEPETQIMRKLALRFDPHIWVNVHSGMEALFMPYD 241
           NWSVDWGKKEKDYDPYEENPGFAPFSEPETQIMRK+ALRFDPHIWVNVHSGMEALFMPYD
Sbjct: 256 NWSVDWGKKEKDYDPYEENPGFAPFSEPETQIMRKIALRFDPHIWVNVHSGMEALFMPYD 315

Query: 242 HKNITPDGEISQQMKLLLEELNILHCHSRCMIGSGGGSVGYLAHGTATDFMFDKARVPMA 301
           HKN TPDGEI+QQMKLLLEELN LHCHSRCMIGSGGGSVGYLAHGTATDFMFDKARVPMA
Sbjct: 316 HKNTTPDGEIAQQMKLLLEELNHLHCHSRCMIGSGGGSVGYLAHGTATDFMFDKARVPMA 375

Query: 302 FTFEIYGDEAATSKDCFRMFNPTDPNTFDRVLGDWSAAFFTIFKMGPEYLDETEFRPKSN 361
           FTFEIYGDEAA+SKDCFRMFNPTDPNTF+RVL DWSAAFFTIFKMGPEYLDETEF PKSN
Sbjct: 376 FTFEIYGDEAASSKDCFRMFNPTDPNTFNRVLSDWSAAFFTIFKMGPEYLDETEFHPKSN 435

Query: 362 IDKLVSIDEYLEGYLIERSSRYGKKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSRISK 421
           +DKLVSIDEYLEGYLIERSSRYGKKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSRISK
Sbjct: 436 VDKLVSIDEYLEGYLIERSSRYGKKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSRISK 495

Query: 422 NKFTRPLVSAISI 434
           NKFTRPLVSAISI
Sbjct: 496 NKFTRPLVSAISI 507

BLAST of CsGy6G018160 vs. ExPASy TrEMBL
Match: A0A5A7TA59 (Metallocarboxypeptidase A-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold119G00240 PE=3 SV=1)

HSP 1 Score: 800 bits (2067), Expect = 8.28e-292
Identity = 394/410 (96.10%), Postives = 401/410 (97.80%), Query Frame = 0

Query: 25  MCLFVTADDPNRLTPINRDLYHSSVDLMKEIEALVHRHSDKLTIETMKSKNKGYAAEIPV 84
           MCLFVTADDP+RLTPIN DLYHSSVDLMKEIEA+VHRH DKLTIETMKSKNKGYAAEIPV
Sbjct: 1   MCLFVTADDPDRLTPINHDLYHSSVDLMKEIEAMVHRHPDKLTIETMKSKNKGYAAEIPV 60

Query: 85  VTYRHGRNNIDDTSKFRILLSFGQHGRELITTEVALRILLILSEEHLLPHMDRASLNNTL 144
           VTYR GRNNIDDTSKFRILLSFGQHGRELITTEVALRILLILSEEHLLPHMD ASLNNTL
Sbjct: 61  VTYRRGRNNIDDTSKFRILLSFGQHGRELITTEVALRILLILSEEHLLPHMDHASLNNTL 120

Query: 145 AKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNRNWSVDWGKKEKDYDPYEENPGFA 204
            KLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNRNWSVDWGKKEKDYDPYEENPGFA
Sbjct: 121 DKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVDLNRNWSVDWGKKEKDYDPYEENPGFA 180

Query: 205 PFSEPETQIMRKLALRFDPHIWVNVHSGMEALFMPYDHKNITPDGEISQQMKLLLEELNI 264
           PFSEPETQIMRK+ALRFDPHIWVNVHSGMEALFMPYDHKN TPDGEI+QQMKLLLEELN 
Sbjct: 181 PFSEPETQIMRKIALRFDPHIWVNVHSGMEALFMPYDHKNTTPDGEIAQQMKLLLEELNH 240

Query: 265 LHCHSRCMIGSGGGSVGYLAHGTATDFMFDKARVPMAFTFEIYGDEAATSKDCFRMFNPT 324
           LHCHSRCMIGSGGGSVGYLAHGTATDFMFDKARVPMAFTFEIYGDEAA+SKDCFRMFNPT
Sbjct: 241 LHCHSRCMIGSGGGSVGYLAHGTATDFMFDKARVPMAFTFEIYGDEAASSKDCFRMFNPT 300

Query: 325 DPNTFDRVLGDWSAAFFTIFKMGPEYLDETEFRPKSNIDKLVSIDEYLEGYLIERSSRYG 384
           DPNTF+RVL DWSAAFFTIFKMGPEYLDETEF PKSN+DKLVSIDEYLEGYLIERSSRYG
Sbjct: 301 DPNTFNRVLSDWSAAFFTIFKMGPEYLDETEFHPKSNVDKLVSIDEYLEGYLIERSSRYG 360

Query: 385 KKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSRISKNKFTRPLVSAISI 434
           KKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSRISKNKFTRPLVSAISI
Sbjct: 361 KKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSRISKNKFTRPLVSAISI 410

BLAST of CsGy6G018160 vs. ExPASy TrEMBL
Match: A0A6J1KIA8 (metallocarboxypeptidase A-like protein MCYG_01475 OS=Cucurbita maxima OX=3661 GN=LOC111496034 PE=3 SV=1)

HSP 1 Score: 763 bits (1971), Expect = 9.35e-277
Identity = 379/436 (86.93%), Postives = 402/436 (92.20%), Query Frame = 0

Query: 1   MGRPFSSFFPSLWVTFFFFFFTSFMCLFVTADDP--NRLTPINRDLYHSSVDLMKEIEAL 60
           M RP  S   + +  FFFFFFT F C  V A+DP  NRLTPINRDLYHSSVDLMKEIEA+
Sbjct: 1   MARPLRSLCLTFFFFFFFFFFTFFDCFLVAAEDPDNNRLTPINRDLYHSSVDLMKEIEAV 60

Query: 61  VHRHSDKLTIETMKSKNKGYAAEIPVVTYRHGRNNIDDTSKFRILLSFGQHGRELITTEV 120
           VHRHSDKLTIETMKS+NKGY  E+PVVTYRHGRNNIDD SKFRILLSFGQHGRELITTE+
Sbjct: 61  VHRHSDKLTIETMKSRNKGYVVEMPVVTYRHGRNNIDDISKFRILLSFGQHGRELITTEL 120

Query: 121 ALRILLILSEEHLLPHMDRASLNNTLAKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVD 180
           ALRILLILSEE  LPHMDRASLNNTL KLVIKVVPMENLNGRRLVEGGDLCERRNGRGVD
Sbjct: 121 ALRILLILSEELFLPHMDRASLNNTLTKLVIKVVPMENLNGRRLVEGGDLCERRNGRGVD 180

Query: 181 LNRNWSVDWGKKEKDYDPYEENPGFAPFSEPETQIMRKLALRFDPHIWVNVHSGMEALFM 240
           LNRNWSVDWGKKEKDYDPYEENPG +PFSEPETQIMRKLAL FDPHIWVNVHSGMEALFM
Sbjct: 181 LNRNWSVDWGKKEKDYDPYEENPGLSPFSEPETQIMRKLALTFDPHIWVNVHSGMEALFM 240

Query: 241 PYDHKNITPDGEISQQMKLLLEELNILHCHSRCMIGSGGGSVGYLAHGTATDFMFDKARV 300
           PYDHKN TPDGEI+QQMKLLLEELN LHCH RCMIGSGGGSVGYLAHGTATDFMFDKARV
Sbjct: 241 PYDHKNTTPDGEIAQQMKLLLEELNNLHCHHRCMIGSGGGSVGYLAHGTATDFMFDKARV 300

Query: 301 PMAFTFEIYGDEAATSKDCFRMFNPTDPNTFDRVLGDWSAAFFTIFKMGPEYLDETEFRP 360
           PM+FTFEIYGD AA+S DCF+MFNPTDP+TF+RVLGDWSAAFFTIFK+GPEYL ET+   
Sbjct: 301 PMSFTFEIYGDAAASSNDCFKMFNPTDPDTFNRVLGDWSAAFFTIFKLGPEYLGETKLHR 360

Query: 361 KSNIDKLVSIDEYLEGYLIERSSRYGKKREVFDLGMQEMRTYFRLFLLSSVLLMFMFCSR 420
           K N+DKLVSIDEYLEGYLIERSSRYGKKREVF+LG+QEMRTYFRLFLLSSVLLMFMFCSR
Sbjct: 361 KGNLDKLVSIDEYLEGYLIERSSRYGKKREVFELGLQEMRTYFRLFLLSSVLLMFMFCSR 420

Query: 421 ISKNKFTRPLVSAISI 434
           ISK+KFT+PLVSA+S+
Sbjct: 421 ISKSKFTKPLVSALSL 436

BLAST of CsGy6G018160 vs. ExPASy TrEMBL
Match: A0A6J1GHD9 (metallocarboxypeptidase A-like protein TRV_02598 OS=Cucurbita moschata OX=3662 GN=LOC111454214 PE=3 SV=1)

HSP 1 Score: 759 bits (1960), Expect = 5.34e-275
Identity = 381/438 (86.99%), Postives = 399/438 (91.10%), Query Frame = 0

Query: 1   MGRPFSSFFPSLWVTFFFFFF---TSFMCLFVTADDP--NRLTPINRDLYHSSVDLMKEI 60
           M RP  S  PSL + FFFFFF   T F C  V A DP  NRLTPINRDLYHSSVDLMKEI
Sbjct: 1   MARPLRSLLPSLCLAFFFFFFFFFTFFDCFLVAAQDPDNNRLTPINRDLYHSSVDLMKEI 60

Query: 61  EALVHRHSDKLTIETMKSKNKGYAAEIPVVTYRHGRNNIDDTSKFRILLSFGQHGRELIT 120
           EA+VHRHSDKLTIETMKS+NKGY  E+PVVTYRHGRNNIDD SKFRILLSFGQHGRELIT
Sbjct: 61  EAVVHRHSDKLTIETMKSRNKGYVVEMPVVTYRHGRNNIDDISKFRILLSFGQHGRELIT 120

Query: 121 TEVALRILLILSEEHLLPHMDRASLNNTLAKLVIKVVPMENLNGRRLVEGGDLCERRNGR 180
           TE+ALRILLILSEE  LPHMDRASLNNTL KLVIKVVPMENLNGRRLVEGGDLCERRNGR
Sbjct: 121 TELALRILLILSEELFLPHMDRASLNNTLNKLVIKVVPMENLNGRRLVEGGDLCERRNGR 180

Query: 181 GVDLNRNWSVDWGKKEKDYDPYEENPGFAPFSEPETQIMRKLALRFDPHIWVNVHSGMEA 240
           GVDLNRNWSVDWGKKEKDYDPYEENPG +PFSEPETQIMRKLAL FDPHIWVNVHSGMEA
Sbjct: 181 GVDLNRNWSVDWGKKEKDYDPYEENPGLSPFSEPETQIMRKLALTFDPHIWVNVHSGMEA 240

Query: 241 LFMPYDHKNITPDGEISQQMKLLLEELNILHCHSRCMIGSGGGSVGYLAHGTATDFMFDK 300
           LFMPYDHKN TPDGEI+QQMKLLLEELN LHCH RCMIGSGGGSVGYLAHGTATDFMFDK
Sbjct: 241 LFMPYDHKNTTPDGEIAQQMKLLLEELNNLHCHHRCMIGSGGGSVGYLAHGTATDFMFDK 300

Query: 301 ARVPMAFTFEIYGDEAATSKDCFRMFNPTDPNTFDRVLGDWSAAFFTIFKMGPEYLDETE 360
           ARVPM+FTFEIYGD AA+S DCF+MFNPTDP TF+RVLGDWSAAFFTIFK+GP YL E +
Sbjct: 301 ARVPMSFTFEIYGDAAASSNDCFKMFNPTDPATFNRVLGDWSAAFFTIFKLGPGYLGEIK 360

Query: 361 FRPKSNIDKLVSIDEYLEGYLIERSSRYGKKREVFDLGMQEMRTYFRLFLLSSVLLMFMF 420
              K N+DKLVSIDEYLEGYLIERSSRYGKKREVF+LG+QEMRTYFRLFLLSSVLLMFMF
Sbjct: 361 LHRKGNLDKLVSIDEYLEGYLIERSSRYGKKREVFELGLQEMRTYFRLFLLSSVLLMFMF 420

Query: 421 CSRISKNKFTRPLVSAIS 433
           CSRISKNKFT+PLVSA+S
Sbjct: 421 CSRISKNKFTKPLVSALS 438

BLAST of CsGy6G018160 vs. TAIR 10
Match: AT5G42320.1 (Zn-dependent exopeptidases superfamily protein )

HSP 1 Score: 558.9 bits (1439), Expect = 3.6e-159
Identity = 270/375 (72.00%), Postives = 310/375 (82.67%), Query Frame = 0

Query: 52  MKEIEALVHRHSDKLTIETMKSKNKGYAAEIPVVTYRHGRNNIDDTSKFRILLSFGQHGR 111
           M++I +LVHRH DKL+IE +KS NKGY AE+ VVTY  G    DD S FRILL+FGQHGR
Sbjct: 1   MEQIHSLVHRHPDKLSIELIKSGNKGYNAEVNVVTYCRGGKESDDRSNFRILLTFGQHGR 60

Query: 112 ELITTEVALRILLILSEEHLLPHMDRASLNNTLAKLVIKVVPMENLNGRRLVEGGDLCER 171
           ELIT+E+A RIL ILSEE  LP+ +   L NTL KLVIK+VP+EN NGR+ VE GDLCER
Sbjct: 61  ELITSELAFRILSILSEEQFLPNKNGGILKNTLDKLVIKMVPIENPNGRKRVESGDLCER 120

Query: 172 RNGRGVDLNRNWSVDWGKKEKDYDPYEENPGFAPFSEPETQIMRKLALRFDPHIWVNVHS 231
           RNGRGVDLNRNW VDWGKKEKDYDP EENPG APFSEPETQIMRKLA+ FDPHIW+NVHS
Sbjct: 121 RNGRGVDLNRNWGVDWGKKEKDYDPSEENPGTAPFSEPETQIMRKLAISFDPHIWINVHS 180

Query: 232 GMEALFMPYDHKNITPDGEISQQMKLLLEELNILHCHSRCMIGSGGGSVGYLAHGTATDF 291
           GMEALFMPYDHKNITP+G  SQ+M+ LLE+LN  HCH RCMIGSGGGSVGYLAHGTATD+
Sbjct: 181 GMEALFMPYDHKNITPEGLPSQKMRTLLEKLNKFHCHDRCMIGSGGGSVGYLAHGTATDY 240

Query: 292 MFDKARVPMAFTFEIYGDEAATSKDCFRMFNPTDPNTFDRVLGDWSAAFFTIFKMGPEYL 351
           ++D  + PMAFTFEIYGD    S+DCF+MFNP D   F RVL DWSAAFFTIF++GP +L
Sbjct: 241 IYDVVKAPMAFTFEIYGDNQTASRDCFKMFNPVDLPNFKRVLNDWSAAFFTIFQLGPLHL 300

Query: 352 DETEFRPKSNIDKLVSIDEYLEGYLIERSSRYGKKREVFDLGMQEMRTYFRLFLLSSVLL 411
           D    +     DK VSIDEYL+GYL+ER +RYGK  EV D+GMQE++TYFRLFLLSSVLL
Sbjct: 301 DGNTSKA---ADKWVSIDEYLDGYLVERKNRYGKNMEVIDVGMQEIKTYFRLFLLSSVLL 360

Query: 412 MFMFCSRISKNKFTR 427
           MFMFCSRI+K+K++R
Sbjct: 361 MFMFCSRIAKSKYSR 372

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5B0114.7e-0723.42Putative metallocarboxypeptidase ecm14 OS=Emericella nidulans (strain FGSC A4 / ... [more]
Q8N4T08.1e-0724.38Carboxypeptidase A6 OS=Homo sapiens OX=9606 GN=CPA6 PE=1 SV=2[more]
Q5U9014.4e-0522.30Carboxypeptidase A6 OS=Mus musculus OX=10090 GN=Cpa6 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_004144231.10.099.77metallocarboxypeptidase A-like protein MCYG_01475 [Cucumis sativus] >KGN47544.1 ... [more]
XP_008464857.12.47e-30195.38PREDICTED: LOW QUALITY PROTEIN: metallocarboxypeptidase A-like protein TRV_02598... [more]
KAA0038457.11.71e-29196.10metallocarboxypeptidase A-like protein [Cucumis melo var. makuwa][more]
XP_038883968.16.45e-28990.62metallocarboxypeptidase A-like protein MCYG_01475 [Benincasa hispida][more]
XP_023002027.11.93e-27686.93metallocarboxypeptidase A-like protein MCYG_01475 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A0A0KFG60.099.77Peptidase_M14 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G3580... [more]
A0A1S3CMF41.20e-30195.38LOW QUALITY PROTEIN: metallocarboxypeptidase A-like protein TRV_02598 OS=Cucumis... [more]
A0A5A7TA598.28e-29296.10Metallocarboxypeptidase A-like protein OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A6J1KIA89.35e-27786.93metallocarboxypeptidase A-like protein MCYG_01475 OS=Cucurbita maxima OX=3661 GN... [more]
A0A6J1GHD95.34e-27586.99metallocarboxypeptidase A-like protein TRV_02598 OS=Cucurbita moschata OX=3662 G... [more]
Match NameE-valueIdentityDescription
AT5G42320.13.6e-15972.00Zn-dependent exopeptidases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000834Peptidase M14, carboxypeptidase ASMARTSM00631zn_carbcoord: 45..316
e-value: 8.8E-36
score: 134.9
IPR000834Peptidase M14, carboxypeptidase APFAMPF00246Peptidase_M14coord: 92..318
e-value: 1.4E-23
score: 84.2
NoneNo IPR availableGENE3D3.40.630.10Zn peptidasescoord: 43..328
e-value: 9.9E-48
score: 164.9
NoneNo IPR availablePANTHERPTHR11705:SF119OS02G0119300 PROTEINcoord: 31..433
NoneNo IPR availablePANTHERPTHR11705PROTEASE FAMILY M14 CARBOXYPEPTIDASE A,Bcoord: 31..433
NoneNo IPR availableSUPERFAMILY53187Zn-dependent exopeptidasescoord: 42..346
IPR034269At5g42320-like, carboxypeptidase domainCDDcd06227M14-CPA-likecoord: 99..321
e-value: 4.1168E-108
score: 316.521

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy6G018160.2CsGy6G018160.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005615 extracellular space
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004181 metallocarboxypeptidase activity
molecular_function GO:0008270 zinc ion binding