CsGy6G014930 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy6G014930
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionTVP38/TMEM64 family membrane protein slr0305-like
LocationGy14Chr6: 13181287 .. 13186660 (+)
RNA-Seq ExpressionCsGy6G014930
SyntenyCsGy6G014930
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGATCCAATTGAGAAGGTCCCAAATTTGACGCCAAAACTGGAGATCAAAGCTGCAAATCTTCGTTGTGAATTCAGAGGAAGTTACAGAGAAAAAGAGTAGGTAATGGTGCTGCTAGTTAATTCTTGTTGTAATTTTCCATTGATGGATGACGATCAAGCTTTCAAATCCTTTTTCACAATATCACTGATTTTCTAAATATATATATATAATATATATATTTTTTCCTTTCCAGTGGTAAAAACCTAGCCAGGAAATCTGGCAAACCTTGGAGGATTGAGGGCTCGCTTGTCGTTGAAGCATTCCATTTCTGATCACAACGACGACGAGGAGGAGTGCAGGCCTTGGCGTCGTCGTCTTTCTATGGCTTTCACATGGCGCTCCGCCCTCAAGATTACCCTCTTACTCCTACTTCTTGCTGCTGCTGTAACTGCCTGTTTTACCCTCCCAATCGAGAAGGTATCAATTTCTTGTTTCTTCCTCATTACCTACTTGAATTGTCGCATTTTCATTTTCATCTGTGCTTCAATTTTTTGAGGTCTGGATTATCTCATCAAATTAATGCTCTCAAGGAATTTCTTGCTTTACGTGTTTGTGTGAATGTTTCAATGTTATACCGTGTATGACGGTTTTTTCGTGTCTAAATGTAGTTTTGATTTTATATGATTTGTATCGATCATTTATTTGAAAATCCAACACCGTTGCAGATTCTGAAGGACTTTTTACTATGGGTTCATGAAGATCTTGGCGCTTGGGGTCCACTTGTGCTGTAAGTCGGTGTAGCTTAGTCCATTGTAGAACATTTGAAGTTGGTTTTAAATTATGTCTGTATCATCGCTTTTGCTTTCCCTCACCACTGGGGATTTATTGTTTTAGGTCTATTGCATACATTCCTTTGACAGTTCTGGCAGTTCCTGCCTCGATACTTACTGTAAGCATCAAATTAATTCTTCTTATACCTGGAAAAGATATAATATGTAGTGATGAAGGTGACTGGAATTGACTTATTTGTCTGTCTTTCAGCTTGGTGGTGGCTATCTGTTTGGGTTACCTGTGGGCTTTGTTGCTGATTCCATTGGTGCAACTGTTGGGGCTGGAGCAGCTTTCCTTCTTGGACGAACTGTAATGTTCGAACCCCTGTGCTTGTCTGTTATTTATATAAGTCTGCGAAACTTTGTATTTGATTATAGAATTATTCTTGGCCTACTTTGTTCTTTCGTTTGTATTTTATAATTGTCAAGTCCTTATTAGATCAAATATGTATGACTGCTAGTTTGTCTGATAATCATTTTATGACATTCCTTACAGATTGGAAAGTCATTTGTTGTTTCCAAGCTGAAGGATTATCCACAGTTTCATTCGGTTGCAATTGCAATCAATCGGTCTGGATTTAAGGTTTGTCCATTTTTGCGTACATCACTTTGTTTGTTTACCGGTGTTTGCATATTATATGGAGGAAAGGAAGAATATCATCTGCGAATTTGTATGCTTCTTTCCTTTTCTGTCATTCAATCTTTGAAATCACTTGCTTCTTCTCTTCCAATGGAAGAGATTTAACCACGTTGTATGTGTGGATTGCAGTTCATTTTCTTCTTTTATTTAATGTATATGAATGCCATTTGATGTTTCATGAATACTAATTCTTGCATATGTCATACATGTAGTGTTTCATAATCTATCTATGAAAATGTCTCCCCTTACAATTGCATGATGGAAGCATATTGAGCTGTAAATGAAATATAAACTAAATTTTGAACTGCAGTCCTAGAGTTGAGAGAGGATGCCAATGAACTTAGTTTGCTAAACTGAAATATAGGATTGCTTTAACTTGTGAGTAGGCTACTCTTGGTGCATGGGGGAAAAGGAAAAGTTAATTTTGTCTTCGTAACTATGAACATGCTGACTGATAAGGAATAGATTTTTATTTTGCTTTCATTAGATTAACATCTCTGGTATTTGTATTTTAAATGACTGAGCTGAGCATGGTACCGTTTGCACTTCTCCCAGTCTACATATCTCACTTGGAATTTGGAAATTATCAGGAATAAGTTATGGATGCTTTTAGTGACCCAAAGTTTTCACTTTTGCAGATCGTTTTGCTACTTCGGCTAGTACCTTTACTTCCATTCAACATGTTAAATTACCTATTATCTGTGACTCCTGTTTCACTAGGGGAGTACATGTTGGCTTCATGGTTGGGAATGATGGTACTTTCTTTATCATGTTTTTATAACATCAATTCTGACCTTGTGTATTTCAGATCACAAATTTGGCATAATTTGTATGTTGATCAGAGTGTTTTATCTAGCCAAGCATAAACTGTTGGTTATTTCTGTTTCATTTGTATGTTCATGCCTTGTTGCAAAATTTATTTAATTTCTCTCCAATTTTTAGTTGTCTGTCCATTTGTAGTGCATAGATAGGCATAATTTTATGTTGCAAAGAGTGATAACTTTTAACTGCATTTATTTTTCTGTCACGACCAGCTAAATAAAAATTGTAGTTTTCAGCCTTTTAGGCTCTTGTTAGTTCATGATGATAACTTCATTGATTACGAAGGAAAATATCATATAGGATTCTGGAGATAGTTTAAGGGGGAGTCTGTTAGGAAGAAAGAAAAGGGGTGCCATCTCCCATGTATAATTCAGATAGCCTTTTGAGACGACTATCTCACACTGGGCACAATTTTGGTACAATAATCTTCCTAGTGATTACTAAATGTTATTCCCATTCCCTAGGCCTGTTTGTCCTGCTAGATAGATAGGTCTGGCTCGTCCAACATCCATGTTCTGTGTTTTTAGTCTTCTTGGATTAACATTTATTATTAAGGAGGGCCTAGCATAATGGTTGCCAATGTAAAGTTATTGTTGAATGCTACAATATTTAGGTAGAGATTGTTCTTCATCCAGGTGAAAGATGGTAATTTTACAAGTTAGCTATTCTTTGTTCGTTTCTGTTCTTCTGCGTGTGGCATGTTTTAGAATCGCCATGATAGAACCAAGGAATTGTGTCTTTTCTTTTAAAATATCTACATCTATCAACCTTAGGAGCTAATTTTAATTACAAATTTTTATCTTTCATCGTCTCTGTTGCAGCCCATCACACTTACATTAGTATATGTTGGAACCACACTCAAAGATCTGTCGGATGTTACACATGGTTGGGGAGAATTTTCAAAGACTCGTTGGGTAAGTACTCATTAACTTCTACTGTATGTGTACATATAAAACAACTCTTTTATCAAGGGGGAAAAAAGAGAATACCCTGTACAAAAATCTAACCGCACAAAAACCCTCTATAGAAACTCAAGTAAAATGCTGTTCAAAGAGTAATTACGAAAGTTTTTCAAAGTTAAAATCTAAAGAGAAATATGACATCTCATCCAGGAACAAATATCACTAGGGTTCCTCTCCACACCTCTAGAAACTTTGTTATTTATCCGTTCCCAATGTCCCATAACAAAGCGCACACCCCAACGTACCATAAAAAAATGGCCTTTTTCGACTAAGAAGCGGACAGAGGAGGAACTCCTTGATTGAGATGTTCTTTAACTTCTACTTTGGGAAGGGAGCGCAACATCTCTCTTTTATGGCTGTTGAGTATCAAGATCTTGATGGCTTCCCCAATAATGTGGAGTTTTTGCTCCCCTATTTAATTTGTTTATGAAATTGCCTTTTCATTTAGAAAAGAAAAACGGAAAAACAGAAGAGGAAAAAGAAAATCAAGATCCTGATGGCATGTTTCTCGCTCGGACAGTTAAAATGTACCACACTATTTTCACGAAATATGAGCGTTCTCGTATATCAAACATATGCTATAGAATACCACTGGTTTTTTGAAGAGCTTCCGAATTCTACACTATATGATATATACACCTTTGTTCTTTCTTGTTTGACGCAATTTATTTCCTTTGCTGTGGATGCAGGCATTCATAATATTGGGCCTTGTAGTATCTGGTAAGTTAATATTATCTGCCGAAACTTGTATCTTTTTTTCTGGGTTTTATTTTCTTGTTCTGTTTCCGGCAAGCAGCTTTTTCTACAAAGATCAAAAGTGGACAATAATTCTAAGAAAAGCGTGTGAGACATTAAGAAAAAAAAGGGAAATGTCCTTTTTAGTCTTATCAAAATAAAGACTTGTGTCTTGTATGCAACATCTGCTACTTGCCAGGAAAAATCTTCGAGCATCTTTGTCAATTAAAATAACATTAGATGGTTCAATAATTAAAAGAAGAAGAAGAAAAAAACATTTGATTATTTGGCTTTAATTTCAAATAAAGTTATAGAAAAAGAGGGAACCGTACCGGGAAGGCTTTTCTGACATTGATTTACATGCAGTGGTTCTGGCGTACTGTGTTACCAGAGTTGCAAAGGCTGCTTTGGAAAAAGCTTTGGCTGAGAACGGAGATGGTGTTGATTATTTGGGTCTCTCCTCGCAGCTACCCATTGTAGATGAATCGCCTGTTGATCTTAACCAGCCTCTCATTATCAAGATTGACTCTCCTCAAGATGATGATCGTAAATGAAAAAACTTAACTCTTTTTCACATGTAAATTCTTCATCCTCCTCCTTCCTTCAATTCAATAGCCCAGGCTTCCCCGGACATTTGAAAACTTTTATTTTTTTATGTATGTACACAATTCATACTCCATAACATAGAATCTAGTTTTTTCATTTCTAAATTAAAAGCATGTATTAAAGTGGTAGCTGCTTGTGTTTTTTTTATTTTTTTTATTTTCCTTTTCCTTTTACGACTGCAATAAAAGTGACAGCCCTATTCATTAAAGTGATTTTCGGCACCGAGTCTGTTTTGTTCATCAGATTGTAATAGGTACATACATATATATGGGGTGAGTTCAACGTTTGGTGGTGAGATTTAAAATTTCAAACATCTTGGTCTATTTGGTTACTAAGCTCACGTTGAAACGTTCTTACCACTAATTTTCCGGTCCCTGATTAGGAAAAAGAAAAGAAAAACTAAAGGGAATTCAAGGCATTCGTTTTTTATTCCTTACTTTCAGATGACATTTGTTGAGACTGTTCGGGTTCATATTGTCATTCCATTTCGGATTTGGTTGTCTGCAATCAACGTCACTGGTTGCCTTTGCTGCGGTGCTGCCCGTGTTGTTGCCAAGCTAGCAAAGTGCTTCATCATAGACGAGCTTATCCTAAGCTTGGTTCTTCAGTTAAATTTCTTAGCATGCAATGATAAAAAAGGGCAATTGTTGAGCCATGAAAGTTGATGTGATTGAAACAGTTATTGTCTGTTCACTTGAATTCATAATTTGCAGGAGAAATCGCCTTTGAATGAAATCATTAATTGATTACCCCGATTATTATTTGTTCACTTGAATTCATAATATTTTCGATAAAATCC

mRNA sequence

CGATCCAATTGAGAAGGTCCCAAATTTGACGCCAAAACTGGAGATCAAAGCTGCAAATCTTCGTTGTGAATTCAGAGGAAGTTACAGAGAAAAAGAGTAGTGGTAAAAACCTAGCCAGGAAATCTGGCAAACCTTGGAGGATTGAGGGCTCGCTTGTCGTTGAAGCATTCCATTTCTGATCACAACGACGACGAGGAGGAGTGCAGGCCTTGGCGTCGTCGTCTTTCTATGGCTTTCACATGGCGCTCCGCCCTCAAGATTACCCTCTTACTCCTACTTCTTGCTGCTGCTGTAACTGCCTGTTTTACCCTCCCAATCGAGAAGATTCTGAAGGACTTTTTACTATGGGTTCATGAAGATCTTGGCGCTTGGGGTCCACTTGTGCTGTCTATTGCATACATTCCTTTGACAGTTCTGGCAGTTCCTGCCTCGATACTTACTCTTGGTGGTGGCTATCTGTTTGGGTTACCTGTGGGCTTTGTTGCTGATTCCATTGGTGCAACTGTTGGGGCTGGAGCAGCTTTCCTTCTTGGACGAACTATTGGAAAGTCATTTGTTGTTTCCAAGCTGAAGGATTATCCACAGTTTCATTCGGTTGCAATTGCAATCAATCGGTCTGGATTTAAGATCGTTTTGCTACTTCGGCTAGTACCTTTACTTCCATTCAACATGTTAAATTACCTATTATCTGTGACTCCTGTTTCACTAGGGGAGTACATGTTGGCTTCATGGTTGGGAATGATGCCCATCACACTTACATTAGTATATGTTGGAACCACACTCAAAGATCTGTCGGATGTTACACATGGTTGGGGAGAATTTTCAAAGACTCGTTGGGCATTCATAATATTGGGCCTTGTAGTATCTGTGGTTCTGGCGTACTGTGTTACCAGAGTTGCAAAGGCTGCTTTGGAAAAAGCTTTGGCTGAGAACGGAGATGGTGTTGATTATTTGGGTCTCTCCTCGCAGCTACCCATTGTAGATGAATCGCCTGTTGATCTTAACCAGCCTCTCATTATCAAGATTGACTCTCCTCAAGATGATGATCGTAAATGAAAAAACTTAACTCTTTTTCACATGTAAATTCTTCATCCTCCTCCTTCCTTCAATTCAATAGCCCAGGCTTCCCCGGACATTTGAAAACTTTTATTTTTTTATGTATGTACACAATTCATACTCCATAACATAGAATCTAGTTTTTTCATTTCTAAATTAAAAGCATGTATTAAAGTGGTAGCTGCTTGTGTTTTTTTTATTTTTTTTATTTTCCTTTTCCTTTTACGACTGCAATAAAAGTGACAGCCCTATTCATTAAAGTGATTTTCGGCACCGAGTCTGTTTTGTTCATCAGATTGTAATAGGTACATACATATATATGGGGTGAGTTCAACGTTTGGTGGTGAGATTTAAAATTTCAAACATCTTGGTCTATTTGGTTACTAAGCTCACGTTGAAACGTTCTTACCACTAATTTTCCGGTCCCTGATTAGGAAAAAGAAAAGAAAAACTAAAGGGAATTCAAGGCATTCGTTTTTTATTCCTTACTTTCAGATGACATTTGTTGAGACTGTTCGGGTTCATATTGTCATTCCATTTCGGATTTGGTTGTCTGCAATCAACGTCACTGGTTGCCTTTGCTGCGGTGCTGCCCGTGTTGTTGCCAAGCTAGCAAAGTGCTTCATCATAGACGAGCTTATCCTAAGCTTGGTTCTTCAGTTAAATTTCTTAGCATGCAATGATAAAAAAGGGCAATTGTTGAGCCATGAAAGTTGATGTGATTGAAACAGTTATTGTCTGTTCACTTGAATTCATAATTTGCAGGAGAAATCGCCTTTGAATGAAATCATTAATTGATTACCCCGATTATTATTTGTTCACTTGAATTCATAATATTTTCGATAAAATCC

Coding sequence (CDS)

ATGGCTTTCACATGGCGCTCCGCCCTCAAGATTACCCTCTTACTCCTACTTCTTGCTGCTGCTGTAACTGCCTGTTTTACCCTCCCAATCGAGAAGATTCTGAAGGACTTTTTACTATGGGTTCATGAAGATCTTGGCGCTTGGGGTCCACTTGTGCTGTCTATTGCATACATTCCTTTGACAGTTCTGGCAGTTCCTGCCTCGATACTTACTCTTGGTGGTGGCTATCTGTTTGGGTTACCTGTGGGCTTTGTTGCTGATTCCATTGGTGCAACTGTTGGGGCTGGAGCAGCTTTCCTTCTTGGACGAACTATTGGAAAGTCATTTGTTGTTTCCAAGCTGAAGGATTATCCACAGTTTCATTCGGTTGCAATTGCAATCAATCGGTCTGGATTTAAGATCGTTTTGCTACTTCGGCTAGTACCTTTACTTCCATTCAACATGTTAAATTACCTATTATCTGTGACTCCTGTTTCACTAGGGGAGTACATGTTGGCTTCATGGTTGGGAATGATGCCCATCACACTTACATTAGTATATGTTGGAACCACACTCAAAGATCTGTCGGATGTTACACATGGTTGGGGAGAATTTTCAAAGACTCGTTGGGCATTCATAATATTGGGCCTTGTAGTATCTGTGGTTCTGGCGTACTGTGTTACCAGAGTTGCAAAGGCTGCTTTGGAAAAAGCTTTGGCTGAGAACGGAGATGGTGTTGATTATTTGGGTCTCTCCTCGCAGCTACCCATTGTAGATGAATCGCCTGTTGATCTTAACCAGCCTCTCATTATCAAGATTGACTCTCCTCAAGATGATGATCGTAAATGA

Protein sequence

MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPLTVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQFHSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLTLVYVGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVDYLGLSSQLPIVDESPVDLNQPLIIKIDSPQDDDRK*
Homology
BLAST of CsGy6G014930 vs. ExPASy Swiss-Prot
Match: Q55909 (TVP38/TMEM64 family membrane protein slr0305 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=slr0305 PE=3 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 5.6e-30
Identity = 76/200 (38.00%), Postives = 123/200 (61.50%), Query Frame = 0

Query: 34  LKDFLLWVHEDLGAWGPLVLSIAYIPLTVLAVPASILTLGGGYLFGLPVGFVADSIGATV 93
           L + L W+ + LG W  +   + Y   TV+ +P SILTLG G +FG+ +G +   IGAT+
Sbjct: 5   LLNALQWI-DGLGTWAAIAFMLLYTVATVVFLPGSILTLGAGVVFGVILGSIYVFIGATL 64

Query: 94  GAGAAFLLGRTIGKSFVVSKLKDYPQFHSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLL 153
           GA AAFL+GR + + +V  K+    +F ++  A+ + G KIV+L RL P+ PFN+LNY  
Sbjct: 65  GATAAFLVGRYLARGWVAKKIAGNQKFKAIDEAVGKEGLKIVILTRLSPVFPFNLLNYAY 124

Query: 154 SVTPVSLGEYMLASWLGMMPITLTLVYVGTTLKDLSDVTHGWGEFSKT-RWAFIILGLVV 213
            +T VSL +Y++ S LGM+P T+  VY+G+    L+ +     + + T +W   I+G + 
Sbjct: 125 GITNVSLKDYVIGS-LGMIPGTIMYVYIGSLAGSLATLGTATNQANPTLQWTIRIVGFIA 184

Query: 214 SVVLAYCVTRVAKAALEKAL 233
           +V +   VT++A+ AL +A+
Sbjct: 185 TVAVTIYVTKIARKALNEAI 202

BLAST of CsGy6G014930 vs. ExPASy Swiss-Prot
Match: Q6YI46 (Transmembrane protein 64 OS=Homo sapiens OX=9606 GN=TMEM64 PE=2 SV=2)

HSP 1 Score: 77.4 bits (189), Expect = 2.8e-13
Identity = 62/226 (27.43%), Postives = 116/226 (51.33%), Query Frame = 0

Query: 12  TLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPLTVLAVPAS--- 71
           +L+L+ + AA+       + + L   LLWV       G L+  + +I   V++ P     
Sbjct: 122 SLVLVCVLAALCFASLALVRRYLHHLLLWVESLDSLLGVLLFVVGFI---VVSFPCGWGY 181

Query: 72  -ILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQFHSV-AIA 131
            +L +  GYL+G  +G     +G  +G   A ++ + +  ++V ++++   +  +V  + 
Sbjct: 182 IVLNVAAGYLYGFVLGMGLMMVGVLIGTFIAHVVCKRLLTAWVAARIQSSEKLSAVIRVV 241

Query: 132 INRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLTLVYVGTTLK 191
              SG K+V L RL P +PF + N + S+T +SL  Y++AS +G++P  L   Y+GTTL+
Sbjct: 242 EGGSGLKVVALARLTP-IPFGLQNAVFSITDLSLPNYLMASSVGLLPTQLLNSYLGTTLR 301

Query: 192 DLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKAL 233
            + DV     E S + +    L +++S+ L + V   A+  L  A+
Sbjct: 302 TMEDVI---AEQSVSGYFVFCLQIIISIGLMFYVVHRAQVELNAAI 340

BLAST of CsGy6G014930 vs. ExPASy Swiss-Prot
Match: Q3U145 (Transmembrane protein 64 OS=Mus musculus OX=10090 GN=Tmem64 PE=1 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 3.7e-13
Identity = 63/226 (27.88%), Postives = 116/226 (51.33%), Query Frame = 0

Query: 12  TLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPLTVLAVPAS--- 71
           +L+L+ + AA+       + + L+  LLWV       G L+  + +I   V++ P     
Sbjct: 123 SLVLVCVLAALCFASLALVRRYLQHLLLWVESLDSLLGVLLFVVGFI---VVSFPCGWGY 182

Query: 72  -ILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQFHSV-AIA 131
            +L +  GYL+G  +G     +G  +G   A ++ + +  ++V +++++  +  +V  + 
Sbjct: 183 IVLNVAAGYLYGFVLGMGLMVVGVLIGTFIAHVVCKRLLTAWVAARIQNSDKLSAVIRVV 242

Query: 132 INRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLTLVYVGTTLK 191
              SG K+V L RL P +PF + N + S+T V L  Y++AS  G++P  L   Y+GTTL+
Sbjct: 243 EGGSGLKVVALARLTP-IPFGLQNAVFSITDVPLPSYLMASSAGLLPTQLLNSYLGTTLR 302

Query: 192 DLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKAL 233
            + DV     E S + +    L +V+S+ L + V   A+  L  A+
Sbjct: 303 TMEDVI---AEQSLSGYFVFCLQIVISIGLMFYVVHRAQVELNAAI 341

BLAST of CsGy6G014930 vs. ExPASy Swiss-Prot
Match: P76219 (TVP38/TMEM64 family membrane protein YdjX OS=Escherichia coli (strain K12) OX=83333 GN=ydjX PE=3 SV=2)

HSP 1 Score: 69.7 bits (169), Expect = 5.8e-11
Identity = 48/166 (28.92%), Postives = 83/166 (50.00%), Query Frame = 0

Query: 10  KITLLLLLLAAAVTACFTLPIEKILKDF--LLWVHEDLGAWGPLVLSIAYIPLTVLAVPA 69
           K     L+ A  + A     +  +L D   L  +    G +G  +  + +I  T+L +P 
Sbjct: 6   KFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSGFFGYSLYILLFIIATLLLLPG 65

Query: 70  SILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQFHSVAIAI 129
           SIL + GG +FG  +G +   I AT+ +  +FLL R +G+  ++  +     F ++   I
Sbjct: 66  SILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARWLGRDLLLKYVGHSNTFQAIEKGI 125

Query: 130 NRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMP 174
            R+G   ++L RL+PL P+N+ NY   +T ++   Y L S L  +P
Sbjct: 126 ARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWPYTLISALTTLP 171

BLAST of CsGy6G014930 vs. ExPASy Swiss-Prot
Match: P9WFS2 (TVP38/TMEM64 family membrane protein MT1538 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=MT1538 PE=3 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 7.9e-08
Identity = 49/171 (28.65%), Postives = 82/171 (47.95%), Query Frame = 0

Query: 12  TLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPLTVLAVPASILT 71
           T++ + +   V     +P    L+D   W  + LGAW PL   + +  +TV   P +  T
Sbjct: 35  TVVGITVLVVVALLVPVPTAVELRD---WA-KSLGAWFPLAFLLVHTVVTVPPFPRTAFT 94

Query: 72  LGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQFHSVAIAINRSG 131
           L  G LFG  VG     +G+T  A  A LL R  G  + ++ L      + +   +   G
Sbjct: 95  LAAGLLFGSVVGVFIAVVGSTASAVIAMLLVRATG--WQLNSLVRRRAINRLDERLRERG 154

Query: 132 FKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLTLVYVG 183
           +  +L LRL+P++PF  +NY    + V +  +  A+  G++P T  +V +G
Sbjct: 155 WLAILSLRLIPVVPFAAINYAAGASGVRILSFAWATLAGLLPGTAAVVILG 199

BLAST of CsGy6G014930 vs. NCBI nr
Match: XP_004150898.1 (uncharacterized protein LOC101204495 [Cucumis sativus] >XP_011657201.1 uncharacterized protein LOC101204495 [Cucumis sativus] >KGN47260.1 hypothetical protein Csa_017041 [Cucumis sativus])

HSP 1 Score: 525 bits (1352), Expect = 1.73e-187
Identity = 275/275 (100.00%), Postives = 275/275 (100.00%), Query Frame = 0

Query: 1   MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL 60
           MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL
Sbjct: 1   MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL 60

Query: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120
           TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF
Sbjct: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120

Query: 121 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLTLVY 180
           HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLTLVY
Sbjct: 121 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLTLVY 180

Query: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVD 240
           VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVD
Sbjct: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVD 240

Query: 241 YLGLSSQLPIVDESPVDLNQPLIIKIDSPQDDDRK 275
           YLGLSSQLPIVDESPVDLNQPLIIKIDSPQDDDRK
Sbjct: 241 YLGLSSQLPIVDESPVDLNQPLIIKIDSPQDDDRK 275

BLAST of CsGy6G014930 vs. NCBI nr
Match: XP_008458555.1 (PREDICTED: TVP38/TMEM64 family membrane protein slr0305-like [Cucumis melo] >XP_008458556.1 PREDICTED: TVP38/TMEM64 family membrane protein slr0305-like [Cucumis melo])

HSP 1 Score: 520 bits (1338), Expect = 2.36e-185
Identity = 273/275 (99.27%), Postives = 273/275 (99.27%), Query Frame = 0

Query: 1   MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL 60
           MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL
Sbjct: 1   MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL 60

Query: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120
           TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF
Sbjct: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120

Query: 121 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLTLVY 180
           HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITL LVY
Sbjct: 121 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVY 180

Query: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVD 240
           VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVD
Sbjct: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVD 240

Query: 241 YLGLSSQLPIVDESPVDLNQPLIIKIDSPQDDDRK 275
           YLGLSSQLPIVDESPVDLNQPLIIKIDSPQ DDRK
Sbjct: 241 YLGLSSQLPIVDESPVDLNQPLIIKIDSPQVDDRK 275

BLAST of CsGy6G014930 vs. NCBI nr
Match: XP_038906539.1 (TVP38/TMEM64 family membrane protein slr0305-like [Benincasa hispida] >XP_038906541.1 TVP38/TMEM64 family membrane protein slr0305-like [Benincasa hispida] >XP_038906542.1 TVP38/TMEM64 family membrane protein slr0305-like [Benincasa hispida] >XP_038906543.1 TVP38/TMEM64 family membrane protein slr0305-like [Benincasa hispida] >XP_038906544.1 TVP38/TMEM64 family membrane protein slr0305-like [Benincasa hispida] >XP_038906545.1 TVP38/TMEM64 family membrane protein slr0305-like [Benincasa hispida])

HSP 1 Score: 496 bits (1276), Expect = 7.40e-176
Identity = 264/278 (94.96%), Postives = 267/278 (96.04%), Query Frame = 0

Query: 1   MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL 60
           MAFTWRSALKIT+ LLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLS+AYIPL
Sbjct: 1   MAFTWRSALKITVFLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSVAYIPL 60

Query: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120
           TVL VPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF
Sbjct: 61  TVLVVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120

Query: 121 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLTLVY 180
           HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITL LVY
Sbjct: 121 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVY 180

Query: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDG-V 240
           VGTTLKDLSDVTHGWGE SKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAE GDG V
Sbjct: 181 VGTTLKDLSDVTHGWGELSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAETGDGAV 240

Query: 241 DYLGLSSQLPIV--DESPVDLNQPLIIKIDSPQDDDRK 275
           DYLGLSSQLPIV  +   VDLNQPLIIKIDSPQDDDRK
Sbjct: 241 DYLGLSSQLPIVAAESHAVDLNQPLIIKIDSPQDDDRK 278

BLAST of CsGy6G014930 vs. NCBI nr
Match: XP_023000292.1 (uncharacterized protein LOC111494568 [Cucurbita maxima])

HSP 1 Score: 491 bits (1265), Expect = 3.15e-174
Identity = 259/275 (94.18%), Postives = 264/275 (96.00%), Query Frame = 0

Query: 1   MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL 60
           MAFTWRSALKIT  LLLLAA VTACFTLPIEKILKDFLLWV +DLGAWGPLVLS+AYIPL
Sbjct: 1   MAFTWRSALKITAALLLLAAVVTACFTLPIEKILKDFLLWVDQDLGAWGPLVLSVAYIPL 60

Query: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120
           TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF
Sbjct: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120

Query: 121 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLTLVY 180
           HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPIT  LVY
Sbjct: 121 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITFALVY 180

Query: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVD 240
           VGTTLKDLSDVTHGWGEFSKTRWAFI+LGLVVSVVLAYCVTRVAKAALEKALAENGD VD
Sbjct: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIMLGLVVSVVLAYCVTRVAKAALEKALAENGDAVD 240

Query: 241 YLGLSSQLPIVDES-PVDLNQPLIIKIDSPQDDDR 274
           YLG+SSQLPIV ES PVDLNQPLIIKIDSP DD +
Sbjct: 241 YLGISSQLPIVAESAPVDLNQPLIIKIDSPPDDSK 275

BLAST of CsGy6G014930 vs. NCBI nr
Match: XP_022930301.1 (uncharacterized protein LOC111436796 [Cucurbita moschata])

HSP 1 Score: 491 bits (1264), Expect = 4.47e-174
Identity = 259/273 (94.87%), Postives = 263/273 (96.34%), Query Frame = 0

Query: 1   MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL 60
           MAFTWRSALKIT  LLLLAA VTACFTLPIEKILKDFLLWV +DLGAWGPLVLS+AYIPL
Sbjct: 1   MAFTWRSALKITAALLLLAAVVTACFTLPIEKILKDFLLWVDQDLGAWGPLVLSVAYIPL 60

Query: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120
           TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF
Sbjct: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120

Query: 121 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLTLVY 180
           HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPIT  LVY
Sbjct: 121 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITFALVY 180

Query: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVD 240
           VGTTLKDLSDVTHGWGEFSKTRWAFI+LGLVVSVVLAYCVTRVAKAALEKALAENGD VD
Sbjct: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIMLGLVVSVVLAYCVTRVAKAALEKALAENGDAVD 240

Query: 241 YLGLSSQLPIVDES-PVDLNQPLIIKIDSPQDD 272
           YLG+SSQLPIV ES PVDLNQPLIIKIDSP DD
Sbjct: 241 YLGISSQLPIVAESAPVDLNQPLIIKIDSPPDD 273

BLAST of CsGy6G014930 vs. ExPASy TrEMBL
Match: A0A0A0KC10 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G237630 PE=4 SV=1)

HSP 1 Score: 525 bits (1352), Expect = 8.38e-188
Identity = 275/275 (100.00%), Postives = 275/275 (100.00%), Query Frame = 0

Query: 1   MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL 60
           MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL
Sbjct: 1   MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL 60

Query: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120
           TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF
Sbjct: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120

Query: 121 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLTLVY 180
           HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLTLVY
Sbjct: 121 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLTLVY 180

Query: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVD 240
           VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVD
Sbjct: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVD 240

Query: 241 YLGLSSQLPIVDESPVDLNQPLIIKIDSPQDDDRK 275
           YLGLSSQLPIVDESPVDLNQPLIIKIDSPQDDDRK
Sbjct: 241 YLGLSSQLPIVDESPVDLNQPLIIKIDSPQDDDRK 275

BLAST of CsGy6G014930 vs. ExPASy TrEMBL
Match: A0A1S3C8Q0 (TVP38/TMEM64 family membrane protein slr0305-like OS=Cucumis melo OX=3656 GN=LOC103497924 PE=4 SV=1)

HSP 1 Score: 520 bits (1338), Expect = 1.14e-185
Identity = 273/275 (99.27%), Postives = 273/275 (99.27%), Query Frame = 0

Query: 1   MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL 60
           MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL
Sbjct: 1   MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL 60

Query: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120
           TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF
Sbjct: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120

Query: 121 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLTLVY 180
           HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITL LVY
Sbjct: 121 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVY 180

Query: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVD 240
           VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVD
Sbjct: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVD 240

Query: 241 YLGLSSQLPIVDESPVDLNQPLIIKIDSPQDDDRK 275
           YLGLSSQLPIVDESPVDLNQPLIIKIDSPQ DDRK
Sbjct: 241 YLGLSSQLPIVDESPVDLNQPLIIKIDSPQVDDRK 275

BLAST of CsGy6G014930 vs. ExPASy TrEMBL
Match: A0A6J1KD88 (uncharacterized protein LOC111494568 OS=Cucurbita maxima OX=3661 GN=LOC111494568 PE=4 SV=1)

HSP 1 Score: 491 bits (1265), Expect = 1.52e-174
Identity = 259/275 (94.18%), Postives = 264/275 (96.00%), Query Frame = 0

Query: 1   MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL 60
           MAFTWRSALKIT  LLLLAA VTACFTLPIEKILKDFLLWV +DLGAWGPLVLS+AYIPL
Sbjct: 1   MAFTWRSALKITAALLLLAAVVTACFTLPIEKILKDFLLWVDQDLGAWGPLVLSVAYIPL 60

Query: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120
           TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF
Sbjct: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120

Query: 121 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLTLVY 180
           HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPIT  LVY
Sbjct: 121 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITFALVY 180

Query: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVD 240
           VGTTLKDLSDVTHGWGEFSKTRWAFI+LGLVVSVVLAYCVTRVAKAALEKALAENGD VD
Sbjct: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIMLGLVVSVVLAYCVTRVAKAALEKALAENGDAVD 240

Query: 241 YLGLSSQLPIVDES-PVDLNQPLIIKIDSPQDDDR 274
           YLG+SSQLPIV ES PVDLNQPLIIKIDSP DD +
Sbjct: 241 YLGISSQLPIVAESAPVDLNQPLIIKIDSPPDDSK 275

BLAST of CsGy6G014930 vs. ExPASy TrEMBL
Match: A0A6J1EQJ6 (uncharacterized protein LOC111436796 OS=Cucurbita moschata OX=3662 GN=LOC111436796 PE=4 SV=1)

HSP 1 Score: 491 bits (1264), Expect = 2.17e-174
Identity = 259/273 (94.87%), Postives = 263/273 (96.34%), Query Frame = 0

Query: 1   MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL 60
           MAFTWRSALKIT  LLLLAA VTACFTLPIEKILKDFLLWV +DLGAWGPLVLS+AYIPL
Sbjct: 1   MAFTWRSALKITAALLLLAAVVTACFTLPIEKILKDFLLWVDQDLGAWGPLVLSVAYIPL 60

Query: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120
           TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF
Sbjct: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120

Query: 121 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLTLVY 180
           HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPIT  LVY
Sbjct: 121 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITFALVY 180

Query: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVD 240
           VGTTLKDLSDVTHGWGEFSKTRWAFI+LGLVVSVVLAYCVTRVAKAALEKALAENGD VD
Sbjct: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIMLGLVVSVVLAYCVTRVAKAALEKALAENGDAVD 240

Query: 241 YLGLSSQLPIVDES-PVDLNQPLIIKIDSPQDD 272
           YLG+SSQLPIV ES PVDLNQPLIIKIDSP DD
Sbjct: 241 YLGISSQLPIVAESAPVDLNQPLIIKIDSPPDD 273

BLAST of CsGy6G014930 vs. ExPASy TrEMBL
Match: A0A6J1H6Q0 (uncharacterized protein LOC111460118 OS=Cucurbita moschata OX=3662 GN=LOC111460118 PE=4 SV=1)

HSP 1 Score: 482 bits (1241), Expect = 6.67e-171
Identity = 252/272 (92.65%), Postives = 260/272 (95.59%), Query Frame = 0

Query: 1   MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL 60
           MAFTWRSALKIT LL+L+AAAV ACFTLPIEKILKDFLLWV +DLGAWGPLVLS+AYIPL
Sbjct: 1   MAFTWRSALKITFLLILVAAAVIACFTLPIEKILKDFLLWVDQDLGAWGPLVLSLAYIPL 60

Query: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120
           TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF
Sbjct: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120

Query: 121 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLTLVY 180
           HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITL LVY
Sbjct: 121 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLALVY 180

Query: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVD 240
           VGTTLKDLSDVTHGWGE SKTR AFIILGLVVS +LAYC+TRVAKAALEKALAENGD VD
Sbjct: 181 VGTTLKDLSDVTHGWGELSKTRLAFIILGLVVSALLAYCITRVAKAALEKALAENGDAVD 240

Query: 241 YLGLSSQLPIVDESPVDLNQPLIIKIDSPQDD 272
           YLGLS +LP+  ESPVDLN PLIIKIDSPQDD
Sbjct: 241 YLGLSPRLPVAAESPVDLNLPLIIKIDSPQDD 272

BLAST of CsGy6G014930 vs. TAIR 10
Match: AT5G19070.1 (SNARE associated Golgi protein family )

HSP 1 Score: 384.0 bits (985), Expect = 9.9e-107
Identity = 196/275 (71.27%), Postives = 234/275 (85.09%), Query Frame = 0

Query: 1   MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL 60
           MAFTW SAL+I++LL+L+AA V AC+ LP+EK+LKDFLLWV +DLG WGP  L++AYIPL
Sbjct: 1   MAFTWGSALRISVLLILVAAIVLACYFLPVEKLLKDFLLWVEQDLGPWGPFALAVAYIPL 60

Query: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120
           TVLAVPAS+LTLGGGYLFGLP+GFVADS+GAT+G+GAAFLLGRTIGK FVV+KLKDYPQF
Sbjct: 61  TVLAVPASVLTLGGGYLFGLPIGFVADSVGATLGSGAAFLLGRTIGKPFVVAKLKDYPQF 120

Query: 121 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLTLVY 180
            SVA+AI +SGFKI LLLRL PLLPF+MLNYLLSVTP+ LG Y+L+SWLGMMPITL LVY
Sbjct: 121 QSVALAIEKSGFKICLLLRLAPLLPFSMLNYLLSVTPIRLGPYLLSSWLGMMPITLALVY 180

Query: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAEN-GDGV 240
           VGTTLKDLSDVTH W EFS  RWAF+I  LV+SV+L  CVT+VAK AL KALAE+ GD  
Sbjct: 181 VGTTLKDLSDVTHKWSEFSPGRWAFLISSLVISVILMVCVTKVAKDALRKALAEHGGDMN 240

Query: 241 DYLGLSSQLPIVDESPVDLNQPLIIKIDSPQDDDR 275
           + +    +L + D++  DLN+PL+IKID+ Q  D+
Sbjct: 241 EAVAALPELTVTDDASTDLNEPLLIKIDAQQPQDQ 275

BLAST of CsGy6G014930 vs. TAIR 10
Match: AT1G03260.1 (SNARE associated Golgi protein family )

HSP 1 Score: 342.4 bits (877), Expect = 3.3e-94
Identity = 181/269 (67.29%), Postives = 223/269 (82.90%), Query Frame = 0

Query: 1   MAFTWRSALKITLLLLLLAAAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIPL 60
           M+FT  S  +I + LLLL A V+A   LP+E+ LKDFLLW+ EDLG +GPL L++AYIPL
Sbjct: 4   MSFT-PSTFRIAISLLLLVAIVSAVIFLPVEQKLKDFLLWIKEDLGPFGPLALALAYIPL 63

Query: 61  TVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQF 120
           T++AVPAS+LTLGGGYLFGLPVGFVADS+GAT+GA AAFLLGRTIGKS+V SK+K YP+F
Sbjct: 64  TIVAVPASVLTLGGGYLFGLPVGFVADSLGATLGATAAFLLGRTIGKSYVTSKIKHYPKF 123

Query: 121 HSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLTLVY 180
            +V++AI +SGFKIVLLLR+VP+LPFNMLNYLLSVTPV LGEYMLA+WLGMMPIT  LVY
Sbjct: 124 QAVSVAIQKSGFKIVLLLRVVPILPFNMLNYLLSVTPVRLGEYMLATWLGMMPITFALVY 183

Query: 181 VGTTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAENGDGVD 240
           VGTTLKDLSD+THGW E S  RW  +++G+ ++V+L  C+TRVAK++L+KALAENG  +D
Sbjct: 184 VGTTLKDLSDITHGWHEVSVFRWVIMMVGVALAVILIICITRVAKSSLDKALAENGTELD 243

Query: 241 --YLGLSSQLPIVDESPVDLNQPLIIKID 268
                 +S LPI  E P DL +PL+I+ID
Sbjct: 244 GKKNDDASVLPIA-EPPPDLQEPLVIRID 270

BLAST of CsGy6G014930 vs. TAIR 10
Match: AT1G22850.1 (SNARE associated Golgi protein family )

HSP 1 Score: 91.3 bits (225), Expect = 1.3e-18
Identity = 77/234 (32.91%), Postives = 123/234 (52.56%), Query Frame = 0

Query: 7   SALKITLL--LLLLA-----AAVTACFTLPIEKILKDFLLWVHEDLGAWGPLVLSIAYIP 66
           +A+K T+L  +LL+      A V   +   I   L  F  ++ E  G  G  +    Y  
Sbjct: 104 AAIKGTILAGVLLIGTVGGFAGVGYVYRDQINTFLTQFSTYI-EGYGTAGYALFIAVYAG 163

Query: 67  LTVLAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQ 126
           L +LA+PA  LT+  G LFG  +G +  SI  T+ A  AFL+ R   +  ++  ++D  +
Sbjct: 164 LEILAIPALPLTMSAGLLFGPLIGTIIVSISGTMAASVAFLIARYFARERILKLVEDNKK 223

Query: 127 FHSVAIAINRSGFKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLTLV 186
           F ++  AI  +GF++V LLRL PLLPF++ NYL  +T V    Y+L SWLGM+P +   V
Sbjct: 224 FLAIDKAIGENGFRVVTLLRLSPLLPFSLGNYLYGLTSVKFVPYVLGSWLGMLPGSWAYV 283

Query: 187 YVG----TTLKDLSDVTHGWGEFSKTRWAFIILGLVVSVVLAYCVTRVAKAALE 230
             G      +++ S+V    G     +   + +GL+V+ +    VT +AK A++
Sbjct: 284 SAGAFGRAIIQEESNVGLPGG---NGQLLTLGVGLLVTALAGTYVTSLAKDAIK 333

BLAST of CsGy6G014930 vs. TAIR 10
Match: AT1G12450.1 (SNARE associated Golgi protein family )

HSP 1 Score: 55.5 bits (132), Expect = 8.1e-08
Identity = 62/202 (30.69%), Postives = 92/202 (45.54%), Query Frame = 0

Query: 30  IEKILKDFLLWVHE--DLGAWGPLVL-SIAYIPLTVLAVPASILTLGGGYLFGLPVGFVA 89
           IEK L  F+ WV     +   G L+  S+A  P  +L  P+S      G  FG   GF+ 
Sbjct: 85  IEKELIPFINWVRNTFSIPVLGLLLFASVALFPSILL--PSSPSMWMAGLTFGYGKGFLL 144

Query: 90  DSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQFHSVAIAINRS----GFKIVLLLRLVP 149
               A++G    FL+G       +   LK YP+  ++  A         F+ V L+R+ P
Sbjct: 145 ILSAASIGVTLPFLIGHLFLHK-MQEWLKKYPKKAAILRAAGEGTWFHQFQAVTLIRVSP 204

Query: 150 LLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLTLVYVGTTLKDLSDVTHGWGEFSKTR 209
             P+ + NY    T V  G Y+L S +GM+P     +Y G  L+ L+         S TR
Sbjct: 205 -FPYIIYNYCALATGVHYGPYILGSLVGMVPEIFVSIYTGIMLRTLAVA-------SDTR 264

Query: 210 WAFIILGLVVSVVLAYCVTRVA 225
               ++ +VV+ VL +CVT  A
Sbjct: 265 HTLSVVEIVVN-VLGFCVTASA 274

BLAST of CsGy6G014930 vs. TAIR 10
Match: AT4G22850.1 (SNARE associated Golgi protein family )

HSP 1 Score: 49.3 bits (116), Expect = 5.8e-06
Identity = 63/238 (26.47%), Postives = 102/238 (42.86%), Query Frame = 0

Query: 9   LKITLLLLLLAAAVTACF----TLPIEKILKDFLLWVHEDL--GAWGPLVLSIAYIPLTV 68
           +K++LL   LAA     +     L ++K L   + W          G LV +   I  T+
Sbjct: 57  VKLSLLFAFLAALAVVGYIWIGPLIMDKELIPLIQWEIRTFTHPVCGLLVFASVAIFPTI 116

Query: 69  LAVPASILTLGGGYLFGLPVGFVADSIGATVGAGAAFLLGRTIGKSFVVSKLKDYPQFHS 128
           L +P++      G  FG   GF+     A VG    + +G+      +   L+ YP   +
Sbjct: 117 L-LPSTPSMWIAGMTFGYGYGFLLIISAAAVGVSLPYFIGQLFCHK-IQGWLERYPDQAA 176

Query: 129 VAIAINRSG----FKIVLLLRLVPLLPFNMLNYLLSVTPVSLGEYMLASWLGMMPITLTL 188
           V  A         F +V L+R+ P  P+ + NY    T V  G Y+  S LGM+P     
Sbjct: 177 VLRAAGEGNWLHQFLLVTLIRISP-FPYILYNYCSVATRVKYGPYITGSLLGMVPEVFVA 236

Query: 189 VYVGTTLKDLSDVTHGWGE-FSKTRWAFIILGLVVSVVLAYCVTRVAKAALEKALAEN 236
           +Y G  ++ L++ +    +  S T+    ILG + +V     +T+ AK  LE    E+
Sbjct: 237 IYTGILVRTLAEASSAEEQGLSVTQVILNILGFLATVATTILITKYAKRQLETMKKED 291

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q559095.6e-3038.00TVP38/TMEM64 family membrane protein slr0305 OS=Synechocystis sp. (strain PCC 68... [more]
Q6YI462.8e-1327.43Transmembrane protein 64 OS=Homo sapiens OX=9606 GN=TMEM64 PE=2 SV=2[more]
Q3U1453.7e-1327.88Transmembrane protein 64 OS=Mus musculus OX=10090 GN=Tmem64 PE=1 SV=1[more]
P762195.8e-1128.92TVP38/TMEM64 family membrane protein YdjX OS=Escherichia coli (strain K12) OX=83... [more]
P9WFS27.9e-0828.65TVP38/TMEM64 family membrane protein MT1538 OS=Mycobacterium tuberculosis (strai... [more]
Match NameE-valueIdentityDescription
XP_004150898.11.73e-187100.00uncharacterized protein LOC101204495 [Cucumis sativus] >XP_011657201.1 uncharact... [more]
XP_008458555.12.36e-18599.27PREDICTED: TVP38/TMEM64 family membrane protein slr0305-like [Cucumis melo] >XP_... [more]
XP_038906539.17.40e-17694.96TVP38/TMEM64 family membrane protein slr0305-like [Benincasa hispida] >XP_038906... [more]
XP_023000292.13.15e-17494.18uncharacterized protein LOC111494568 [Cucurbita maxima][more]
XP_022930301.14.47e-17494.87uncharacterized protein LOC111436796 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A0A0KC108.38e-188100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G237630 PE=4 SV=1[more]
A0A1S3C8Q01.14e-18599.27TVP38/TMEM64 family membrane protein slr0305-like OS=Cucumis melo OX=3656 GN=LOC... [more]
A0A6J1KD881.52e-17494.18uncharacterized protein LOC111494568 OS=Cucurbita maxima OX=3661 GN=LOC111494568... [more]
A0A6J1EQJ62.17e-17494.87uncharacterized protein LOC111436796 OS=Cucurbita moschata OX=3662 GN=LOC1114367... [more]
A0A6J1H6Q06.67e-17192.65uncharacterized protein LOC111460118 OS=Cucurbita moschata OX=3662 GN=LOC1114601... [more]
Match NameE-valueIdentityDescription
AT5G19070.19.9e-10771.27SNARE associated Golgi protein family [more]
AT1G03260.13.3e-9467.29SNARE associated Golgi protein family [more]
AT1G22850.11.3e-1832.91SNARE associated Golgi protein family [more]
AT1G12450.18.1e-0830.69SNARE associated Golgi protein family [more]
AT4G22850.15.8e-0626.47SNARE associated Golgi protein family [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032816SNARE associated Golgi proteinPFAMPF09335SNARE_assoccoord: 65..183
e-value: 1.9E-24
score: 86.4
IPR015414Transmembrane protein TMEM64PANTHERPTHR12677UNCHARACTERIZEDcoord: 6..267
NoneNo IPR availablePANTHERPTHR12677:SF51SUBFAMILY NOT NAMEDcoord: 6..267
NoneNo IPR availablePROSITEPS51257PROKAR_LIPOPROTEINcoord: 1..25
score: 5.0

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy6G014930.2CsGy6G014930.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane