CsGy6G014850 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy6G014850
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionAdaptin ear-binding coat-associated protein 1 NECAP-1 isoform 2
LocationGy14Chr6: 13071780 .. 13076512 (+)
RNA-Seq ExpressionCsGy6G014850
SyntenyCsGy6G014850
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCCACCCCACGATCAACAGAGGATGAAAAGTTGACTGCCGCCACGGTTTCCAAAATCTAACGGTCAACTATTTTTAGAGAAAATGGTTGCATGTAGTACCGAACGCAGAGAATCGTGGGTTACTTCGTGTTTGGCCGATCTTTCCATCTTCTTTGAAGCAAAGAGAAGAGGAGAGAAGAGAAGGGAAGGCAACTGAAAGCGAGTTGTTTCCACATCCAATCACTCACCTCATTTTCCAATCCAACTTCAAAATTCCTCTATTCATTTTAATTTTCTCAAACCCATCTTTCGTTTCAACGAATTTCTTTTTCCTATTTCTCTGATCTCGGTTTTGATCTCACGATCTCTCCAATCTCCTTCTTATTTCTCCATGGAGGAGCAGCAGCAGAAGACGCCGAAGAATCAAGAAATCCAAGATGATCACGAAGACAATGAAGCAATTGAGCTTGTTCTCTTTCAAGTTCCGGAATGTTATGTCTATTTGGTGAGTCAAATTATGCCCTGCGCCTCTCACACAAATCCAGGTTCTTAGTTACTTCTGATGCATAATTCTTAAGCTTTCTGTGGCCTTCTTTTGTGATTACTCAAATGTGAACTCGTTGTTAGCACTGTACTTGATTGTATAGTATACTCTTGGGAATTGCTGATATCATGTTTTGGCATTTTTTTGTGCATTTGTCGTATCGGTATCTTCCCTTTCTTGTGCTTACGTCCTATTTCTACGTTTGGAAATCATGCAATTGAGGTATCTTCGTTATGAAGATGTTTCATATCTCGTTGGTGCGTGTAATTTTTGTTTGTGGTGCTGCGTTTCTGTATCCACATTTTGCCTTCTTGGTGGATGACGTTTGTATGAAAAATGTGAGAAAACTAATGTACTGTATTTGCTGGTTTTTGACTTCTTTATAATCTAAAAATACAACATATTTTTGTGTGTATGTATGATGGTATTAGGTTCTGTTGGTTATTTTATGTCATCAAATTACCTGGTTTTCTAGTACTTAATTTGATTTTTTATGATTTCCTGCAGATACCGCCAAGAAAAACTGCAGCTTCCTACAGGTTTTAATTGTTCCTTGTTAAACAAAGAATAATACTATAAATTGCAATATGTTGACTAATATTGTGCCTCTGTGTCAAATTGGTGATTTCATTATTTGGTTTTCCAATATAATGTCTCTGCTGTTGGCTTATAATTCCTAATATAAGTTATAAGAGAATATAACGAGTAAAAGAATTTGAAAGTACATCTTTAGATCTAGAAATATCTCCCAAGACGTGCATTTGTTCTTTGATGAGTCTTATGTGCTTTTCATATCCTCATACTAGTACAACTTTTATTGCACTGACATAGTTTCTGGCCTTGACTTTAAAATATATGCCCACTCACTCAATAGCTGTTCATTATTATCTTTGGTAGGGCCTGTCAGCACCTAACTAACACTGAATAATGGGAACACAAAGGACCACACTTTAGAACTGAAGTGAAAATGTTACACACCACAATAATAGCTTTTAGCATTCAAGAGTCACTGAACTGTTGTCTCTTACGCTTAGTGATTATGATCGTTCTAGTTATATTGAATGACTAACTGATACGACTGAAATTAAAATGTTCCTTGCCTTTCAAACATGTAGAATCTCTACCTCACCTCAAAGGCAAAAAAAAAATAGTGGCAATAAAAATAAAGTTCTGTTTATTGTTTTATTTCATTTTACCAACGGAAGCTCAGTTTCTATTTTTTTATATATAAAAAAAGTTATCTATTCATTACAATGCAAAATTTGACATTATTATGAATATTCTTTTTGGAATGAAACATAGACATTTTGTTAACATAATGGCATTATAAAAGAGGGAAAAGTCCTATCAGGATGATGATTTTTTTTTTTCAATCGGTCATGATAATAGATTTGCTTAATTACAAAAAGGAGGACCCAATTTACACCAACTAAGGAGACAATAAAACGATAGATGTGAGAAAACCCACATCTAAATTGAGCCAAGGAATCTTCTCTTCTTTCTTCTAAGGATGCCCAGAGTCCCGAATACTTCAAAAACAAAGTCCCAAAAGGTTCTTGCCTGTTTGCACCAAGTAAATGGTTTTGGGATTCCTCATCCTTTTTATATAGAATGCAGCAGTTTGGTGAGATGGATATCCATATCCGTGGAGATCCTTTTTGAAGATTTGTCTTGTTTATTAAGACACAAATGGTTCAACTGCCAAATGAAAAACTTCACCTTTTTGAAATATGGACCTTTCCAGATGGAGAGGTAAAGATCTGTGCAGACAGTATTTCCCCGTTGATGATATGGATCTAATGAGAGATTCTGAAGAAAAAAATCCCTTCTTTTTTCAAATGCCAACACCAAAAATTCATGGATCCATTTAGAGTAACCACATCAAGAGCATCGATAACATTGGTTGATTGAAGATCATAACACGATAGTTTTCTTTTTCCTTCCTTTTTTTTGGTATGTTTTGTGTGTGAGTATATAGGTTTACATTGGTTTGTCCCTAGCTGTGAAGAACAGATACTTAGTTTGATAATATATCTTTATATCATGTTTGATGTTTTATAAATCCACCATTCATGCTCCTATCTAAAAAAATCCACTACATATGTTCACCATTTATGGCTTAGTTATGAAAATCCATTATATACTCGTCATTTAAAGCTTTGTTTTACACTTGTCTTCTGGTTAACTTAAGGGCTGATGAATGGGATGTGAATAAATGGGCTTGGGAGGGAAAACTAAAAGTCCTTAGCAAGGGAGAAGAATGCATTATCAGACTTGAAGATAAGAGCACAGGTGAGACCATCTCTTAGTTTTTTCCTACAAAAGTATTGCGTATCTGTAGTATTAAAATGGTAGGTCAATTAAATAATTGCTGATTAGGTGTAGTTGGTGTGCTTCTAAAGACCAACTGATTATTTTTATAATAGGTGAGTTATATGCTCGGGCGTTCTTGAGAGATGGAGAAAAGCATCCAGTTGAACCTGTAATTGATAGCAGCAGGTTGATTTCATTTTGAATTGAATTCTAATTGATTTAGTTATAACATTCTTTGTTGGCCTAATCTTGTATGCCTAAATTGCAAGTTTTGTCGAGATTGCTAGTCCAAGTTAACTGCTGTTGTAGTTGGCGTCTAAAAGTTAGAGAAGCTCAGTTGTTCAGATGGATATATTTATTGGCATTTTTATCTTGTTTTAACGCTTATTTAAAGATGCAATGTTTGACTATTGCAGATATTTTGTTCTTCGCGTGGAAGAGAATATTGGTGAGATGACCATCTTTTCCACCCACATTTTCGATCTAATAACTGATCTCATTATAGAACACTAGCTGGTTCGCATTTATGTGTAATTTTTAATCTTTGGTCAATTTAGTAATTCATTCTTCCCCCCCTGTCTCACGTGACCTGATGTTCACTCCTTGAATCAAGCAATAACATAAAGTAATACCACAAAATGTTTCAATGAAGTAATTCCTTCACATAAAATGCCATCTGTCTACATTCTTGCATGAATGGCGCTGAAATAGCTGTAATGGGGTACTTATTTGTGAAACATGCGACGGGCTAACACTTGAGTAATCAATAAATGTGAAAGATCTCTATGTATGTATATGCAGATGGTCGCCTCCGGCACGCATTTATTGGCATAGGATTTCGAGAAAGAACCGAGGCTTATGACTTCCAAGCAGCTTTGCACGATCATATGAAGTACATACCTTCTACTCTGCCATTATTTTATTTTTCATCTAGTTGATCTTCCTAAACATAGAATTTGCTTGGGAATCTTTGAATGTTGATTTGTTCTACCATTAGTAAACCTAATGCACTTTTTACATCCATTTTCTGGAAAAAGATATCTCAACAAAAAGAAGAGTGCAGAAGAGATGGAACAACAGTTCCAGAATACATCCTCGGTTGATTATAGTTTGAAAGACGGGGAGACTATAGTACTTCAATTAAAAAGCAGAGTAAGCTCATTTTCAAGTCGTTGGTTGCTTCATTGTGATTTATCTCATCTTAACACTTTCTTTGTTATCATCACCAACAGAAAAGCACTAGCAACGTCAAGAAGTCTACTGAGCTGGATCATGTGAAAGTTTCCTCATTAGAAGAAGAAGGCAACACAAAAAAAGAGTCAATTTTTTGTATCAGACCGCCTCCACCTCCTCCAGCTCCACTTTCACCTGTTGCATCCCAAAAATCTCCTAAAAATCTACCACCAAATATCAATTTGGAAGAACCTTCTAAGCAAAAAGATACGGAACCTGCAAAAGAAGACACCAATGATGCTGAAGTTCAAAGCACACTTGATATACCTGACGACGATTTTGGAGATTTCCAGACAGCTGCGTGAGATGACATGGAACTTTGTACATTCTCGAGTACTCTCATCGATCTTCGGTTTCCAGTAGATTCTGCACTACAAATTGTTTAGTTTTTACAAGAGATCTTATGTACTGTTGTTAAACCTGTGTTGTGAAATTAGCTTGAAGAGTTTCCAATAAAGAGTGATCCCAAAATTATGTGATGTTACCATTCTGGGTGGTTCAATTATAAACGTGACGAAGCCAAGTGAAAGGGACAAAAGAGAATATGGAATATCGTAGAAAGTTCTCTCAAGTTTTATCTTGCTGCCTTTTCATTTCAATCCTGGATGATCAATTTAGATCATTTGTTTTATAGCTACTTTTTTCTAATTCCATTTAAAATCA

mRNA sequence

CCCCACCCCACGATCAACAGAGGATGAAAAGTTGACTGCCGCCACGGTTTCCAAAATCTAACGGTCAACTATTTTTAGAGAAAATGGTTGCATGTAGTACCGAACGCAGAGAATCGTGGGTTACTTCGTGTTTGGCCGATCTTTCCATCTTCTTTGAAGCAAAGAGAAGAGGAGAGAAGAGAAGGGAAGGCAACTGAAAGCGAGTTGTTTCCACATCCAATCACTCACCTCATTTTCCAATCCAACTTCAAAATTCCTCTATTCATTTTAATTTTCTCAAACCCATCTTTCGTTTCAACGAATTTCTTTTTCCTATTTCTCTGATCTCGGTTTTGATCTCACGATCTCTCCAATCTCCTTCTTATTTCTCCATGGAGGAGCAGCAGCAGAAGACGCCGAAGAATCAAGAAATCCAAGATGATCACGAAGACAATGAAGCAATTGAGCTTGTTCTCTTTCAAGTTCCGGAATGTTATGTCTATTTGATACCGCCAAGAAAAACTGCAGCTTCCTACAGGGCTGATGAATGGGATGTGAATAAATGGGCTTGGGAGGGAAAACTAAAAGTCCTTAGCAAGGGAGAAGAATGCATTATCAGACTTGAAGATAAGAGCACAGGTGAGTTATATGCTCGGGCGTTCTTGAGAGATGGAGAAAAGCATCCAGTTGAACCTGTAATTGATAGCAGCAGATATTTTGTTCTTCGCGTGGAAGAGAATATTGATGGTCGCCTCCGGCACGCATTTATTGGCATAGGATTTCGAGAAAGAACCGAGGCTTATGACTTCCAAGCAGCTTTGCACGATCATATGAAATATCTCAACAAAAAGAAGAGTGCAGAAGAGATGGAACAACAGTTCCAGAATACATCCTCGGTTGATTATAGTTTGAAAGACGGGGAGACTATAGTACTTCAATTAAAAAGCAGAAAAAGCACTAGCAACGTCAAGAAGTCTACTGAGCTGGATCATGTGAAAGTTTCCTCATTAGAAGAAGAAGGCAACACAAAAAAAGAGTCAATTTTTTGTATCAGACCGCCTCCACCTCCTCCAGCTCCACTTTCACCTGTTGCATCCCAAAAATCTCCTAAAAATCTACCACCAAATATCAATTTGGAAGAACCTTCTAAGCAAAAAGATACGGAACCTGCAAAAGAAGACACCAATGATGCTGAAGTTCAAAGCACACTTGATATACCTGACGACGATTTTGGAGATTTCCAGACAGCTGCGTGAGATGACATGGAACTTTGTACATTCTCGAGTACTCTCATCGATCTTCGGTTTCCAGTAGATTCTGCACTACAAATTGTTTAGTTTTTACAAGAGATCTTATGTACTGTTGTTAAACCTGTGTTGTGAAATTAGCTTGAAGAGTTTCCAATAAAGAGTGATCCCAAAATTATGTGATGTTACCATTCTGGGTGGTTCAATTATAAACGTGACGAAGCCAAGTGAAAGGGACAAAAGAGAATATGGAATATCGTAGAAAGTTCTCTCAAGTTTTATCTTGCTGCCTTTTCATTTCAATCCTGGATGATCAATTTAGATCATTTGTTTTATAGCTACTTTTTTCTAATTCCATTTAAAATCA

Coding sequence (CDS)

ATGGAGGAGCAGCAGCAGAAGACGCCGAAGAATCAAGAAATCCAAGATGATCACGAAGACAATGAAGCAATTGAGCTTGTTCTCTTTCAAGTTCCGGAATGTTATGTCTATTTGATACCGCCAAGAAAAACTGCAGCTTCCTACAGGGCTGATGAATGGGATGTGAATAAATGGGCTTGGGAGGGAAAACTAAAAGTCCTTAGCAAGGGAGAAGAATGCATTATCAGACTTGAAGATAAGAGCACAGGTGAGTTATATGCTCGGGCGTTCTTGAGAGATGGAGAAAAGCATCCAGTTGAACCTGTAATTGATAGCAGCAGATATTTTGTTCTTCGCGTGGAAGAGAATATTGATGGTCGCCTCCGGCACGCATTTATTGGCATAGGATTTCGAGAAAGAACCGAGGCTTATGACTTCCAAGCAGCTTTGCACGATCATATGAAATATCTCAACAAAAAGAAGAGTGCAGAAGAGATGGAACAACAGTTCCAGAATACATCCTCGGTTGATTATAGTTTGAAAGACGGGGAGACTATAGTACTTCAATTAAAAAGCAGAAAAAGCACTAGCAACGTCAAGAAGTCTACTGAGCTGGATCATGTGAAAGTTTCCTCATTAGAAGAAGAAGGCAACACAAAAAAAGAGTCAATTTTTTGTATCAGACCGCCTCCACCTCCTCCAGCTCCACTTTCACCTGTTGCATCCCAAAAATCTCCTAAAAATCTACCACCAAATATCAATTTGGAAGAACCTTCTAAGCAAAAAGATACGGAACCTGCAAAAGAAGACACCAATGATGCTGAAGTTCAAAGCACACTTGATATACCTGACGACGATTTTGGAGATTTCCAGACAGCTGCGTGA

Protein sequence

MEEQQQKTPKNQEIQDDHEDNEAIELVLFQVPECYVYLIPPRKTAASYRADEWDVNKWAWEGKLKVLSKGEECIIRLEDKSTGELYARAFLRDGEKHPVEPVIDSSRYFVLRVEENIDGRLRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQNTSSVDYSLKDGETIVLQLKSRKSTSNVKKSTELDHVKVSSLEEEGNTKKESIFCIRPPPPPPAPLSPVASQKSPKNLPPNINLEEPSKQKDTEPAKEDTNDAEVQSTLDIPDDDFGDFQTAA*
Homology
BLAST of CsGy6G014850 vs. ExPASy Swiss-Prot
Match: Q681Q7 (Uncharacterized protein At1g03900 OS=Arabidopsis thaliana OX=3702 GN=At1g03900 PE=1 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 3.9e-34
Identity = 90/235 (38.30%), Postives = 131/235 (55.74%), Query Frame = 0

Query: 16  DDHEDNEAIELVLFQVPECYVYLIPPRKTAASYRADEWDVNKWAWEGKLKVLSKGEECII 75
           ++ E+ E  E  L  V E  VY IPPR T+  Y+  EW  +   W G+L+V+S  + C I
Sbjct: 4   EEEEEEETFEHTLLVVREVSVYKIPPRTTSGGYKCGEWLQSDKIWSGRLRVVSCKDRCEI 63

Query: 76  RLEDKSTGELYARAFLRDGEK-HPVEPVIDSSRYFVLRVEENIDGRLRHAFIGIGFRERT 135
           RLED ++G+L+A  F+  G + + VEP +DSSRYFVLR++   DGR ++AFIG+GF ER 
Sbjct: 64  RLEDSNSGDLFAACFVDPGRRENSVEPSLDSSRYFVLRID---DGRGKYAFIGLGFAERN 123

Query: 136 EAYDFQAALHDHMKYLNKKKSAE-----EMEQQFQNTSSVDYSLKDGETIVLQLKSRKST 195
           EA+DF  AL DH KY+ ++K  E     E +       +V++ LK+GETI + +K + +T
Sbjct: 124 EAFDFNVALSDHEKYVRREKEKETGETSESDNHIDIHPAVNHRLKEGETIRINVKPKPTT 183

Query: 196 SNVKKSTELDHVKVSSLEEEGNTKKESIFCIRPP--------PPPPAPLSPVASQ 237
           +            + S    G  K + +    PP        P PP P  PVAS+
Sbjct: 184 NG---------TGMLSAALSGTGKPKPLALAPPPKAAGVTRSPLPPPPNDPVASR 226

BLAST of CsGy6G014850 vs. ExPASy Swiss-Prot
Match: Q6P756 (Adaptin ear-binding coat-associated protein 2 OS=Rattus norvegicus OX=10116 GN=Necap2 PE=1 SV=2)

HSP 1 Score: 134.0 bits (336), Expect = 2.6e-30
Identity = 68/163 (41.72%), Postives = 102/163 (62.58%), Query Frame = 0

Query: 25  ELVLFQVPECYVYLIPPRKTAASYRADEWDVNKWAWEGKLKVLSKGEECIIRLEDKSTGE 84
           E VL   PE +VY IPPR T   YRA EW +++ +W G+L++ +KG+   I+LED+++GE
Sbjct: 7   ESVLCVKPEVHVYRIPPRATNRGYRASEWQLDQPSWSGRLRITAKGKVAYIKLEDRTSGE 66

Query: 85  LYARAFLRDGEKHPVEPVIDSSRYFVLRVEENIDGRLRHAFIGIGFRERTEAYDFQAALH 144
           L+A+A +       VE V DSSRYFV+R+E   DG  R AFIGIGF +R +A+DF  AL 
Sbjct: 67  LFAQAPVDQFPGTAVESVTDSSRYFVIRIE---DGNGRRAFIGIGFGDRGDAFDFNVALQ 126

Query: 145 DHMKYLNKKKSAEEMEQQFQNTSSVDYSLKDGETIVLQLKSRK 188
           DH K++ ++    +  Q       +D   K+G+TI + + + +
Sbjct: 127 DHFKWVKQQCEFAKQAQNPDEGPKLDLGFKEGQTIKINIANMR 166

BLAST of CsGy6G014850 vs. ExPASy Swiss-Prot
Match: Q9NVZ3 (Adaptin ear-binding coat-associated protein 2 OS=Homo sapiens OX=9606 GN=NECAP2 PE=1 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 1.0e-29
Identity = 77/206 (37.38%), Postives = 114/206 (55.34%), Query Frame = 0

Query: 20  DNEAIELVLFQVPECYVYLIPPRKTAASYRADEWDVNKWAWEGKLKVLSKGEECIIRLED 79
           +    E VL   P+ +VY IPPR T   YRA EW +++ +W G+L++ +KG+   I+LED
Sbjct: 2   EESGYESVLCVKPDVHVYRIPPRATNRGYRAAEWQLDQPSWSGRLRITAKGQMAYIKLED 61

Query: 80  KSTGELYARAFLRDGEKHPVEPVIDSSRYFVLRVEENIDGRLRHAFIGIGFRERTEAYDF 139
           +++GEL+A+A +       VE V DSSRYFV+R+E   DG  R AFIGIGF +R +A+DF
Sbjct: 62  RTSGELFAQAPVDQFPGTAVESVTDSSRYFVIRIE---DGNGRRAFIGIGFGDRGDAFDF 121

Query: 140 QAALHDHMKYLNKKKSAEEMEQQFQNTSSVDYSLKDGETIVLQLKSRKSTSNVKKSTELD 199
             AL DH K++ ++    +  Q       +D   K+G+TI L + + K     KK     
Sbjct: 122 NVALQDHFKWVKQQCEFAKQAQNPDQGPKLDLGFKEGQTIKLNIANMK-----KKEGAAG 181

Query: 200 HVKVSSLEEEGNTKKESIFCIRPPPP 226
           + +V      G         + PPPP
Sbjct: 182 NPRVRPASTGG-------LSLLPPPP 192

BLAST of CsGy6G014850 vs. ExPASy Swiss-Prot
Match: Q5E9Q4 (Adaptin ear-binding coat-associated protein 2 OS=Bos taurus OX=9913 GN=NECAP2 PE=2 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 1.3e-29
Identity = 79/214 (36.92%), Postives = 117/214 (54.67%), Query Frame = 0

Query: 25  ELVLFQVPECYVYLIPPRKTAASYRADEWDVNKWAWEGKLKVLSKGEECIIRLEDKSTGE 84
           E VL   P+ +VY IPPR T   YRA EW +++ +W G+L++ +KG+   I+LED+++GE
Sbjct: 7   ESVLCVKPDVHVYRIPPRATNRGYRAAEWQLDQPSWSGRLRITAKGQVAYIKLEDRTSGE 66

Query: 85  LYARAFLRDGEKHPVEPVIDSSRYFVLRVEENIDGRLRHAFIGIGFRERTEAYDFQAALH 144
           L+A+A +       VE V DSSRYFV+R+E   DG  R AFIGIGF +R +A+DF  AL 
Sbjct: 67  LFAQAPVDQFPGTAVESVTDSSRYFVIRIE---DGNGRRAFIGIGFGDRGDAFDFNVALQ 126

Query: 145 DHMKYLNKKKSAEEMEQQFQNTSSVDYSLKDGETIVLQLKSRKSTSNVKKSTELDHVKVS 204
           DH K++ ++    +  Q       +D S K+G+TI L + S K       +         
Sbjct: 127 DHFKWVKQQCEFAKQAQNPDQGPKLDLSFKEGQTIKLNIASMKKKDGAAGTPRARPTSTG 186

Query: 205 SLEEEGNTKKESIFCIRPPPP--PPAPLSPVASQ 237
            L             + PPPP    A L+P++ +
Sbjct: 187 GLS------------LLPPPPGAKTAALAPLSGE 205

BLAST of CsGy6G014850 vs. ExPASy Swiss-Prot
Match: Q9CR95 (Adaptin ear-binding coat-associated protein 1 OS=Mus musculus OX=10090 GN=Necap1 PE=1 SV=2)

HSP 1 Score: 126.7 bits (317), Expect = 4.2e-28
Identity = 89/279 (31.90%), Postives = 141/279 (50.54%), Query Frame = 0

Query: 25  ELVLFQVPECYVYLIPPRKTAASYRADEWDVNKWAWEGKLKVLSKGEECIIRLEDKSTGE 84
           E VL   P+  VY IPPR +   YRA +W +++  W G+L++ SKG+   I+LEDK +GE
Sbjct: 8   ESVLCVKPDVSVYRIPPRASNRGYRASDWKLDQPDWTGRLRITSKGKIAYIKLEDKVSGE 67

Query: 85  LYARAFLRDGEKHPVEPVIDSSRYFVLRVEENIDGRLRHAFIGIGFRERTEAYDFQAALH 144
           L+A+A +       VE V DSSRYFV+R++   DG  R AFIGIGF +R +A+DF  +L 
Sbjct: 68  LFAQAPVEQYPGIAVETVTDSSRYFVIRIQ---DGTGRSAFIGIGFTDRGDAFDFNVSLQ 127

Query: 145 DHMKYLNKKKSAEEMEQQFQNTSSVDYSLKDGETIVLQLKSRKSTSNVKKSTELDHVKVS 204
           DH K++ ++    +  Q+  N   +D   K+G+TI L      S  N+         K  
Sbjct: 128 DHFKWVKQETEISKESQEMDNRPKLDLGFKEGQTIKL------SIGNIT-------AKKG 187

Query: 205 SLEEEGNTKKESIFCIRPPP------PPPAPLSPVASQKSPKNLPPN----------INL 264
              +   +    +  + PPP      PPP+    +++  +P  +P +          ++L
Sbjct: 188 GASKPRASGTGGLSLLPPPPGGKVTIPPPSSSVAISNHVTPPPIPKSNHGGNDSDILLDL 247

Query: 265 EEPSKQKDTEPAKEDTNDAEVQSTLDIPDDDFGDFQTAA 288
           + P+    + PA   T+           +D +GDF TA+
Sbjct: 248 DSPAPVSTSAPAPVSTS-----------NDLWGDFSTAS 259

BLAST of CsGy6G014850 vs. NCBI nr
Match: XP_004150324.1 (uncharacterized protein At1g03900 [Cucumis sativus] >KGN47271.1 hypothetical protein Csa_016981 [Cucumis sativus])

HSP 1 Score: 560 bits (1444), Expect = 4.14e-201
Identity = 286/287 (99.65%), Postives = 287/287 (100.00%), Query Frame = 0

Query: 1   MEEQQQKTPKNQEIQDDHEDNEAIELVLFQVPECYVYLIPPRKTAASYRADEWDVNKWAW 60
           MEEQQQKTPKNQEIQDDHEDNEAIELVLFQVPECYVYLIPPRKTAASYRADEWDVNKWAW
Sbjct: 1   MEEQQQKTPKNQEIQDDHEDNEAIELVLFQVPECYVYLIPPRKTAASYRADEWDVNKWAW 60

Query: 61  EGKLKVLSKGEECIIRLEDKSTGELYARAFLRDGEKHPVEPVIDSSRYFVLRVEENIDGR 120
           EGKLKVLSKGEECIIRLEDKSTGELYARAFLRDGEKHPVEPVIDSSRYFVLRVEENIDGR
Sbjct: 61  EGKLKVLSKGEECIIRLEDKSTGELYARAFLRDGEKHPVEPVIDSSRYFVLRVEENIDGR 120

Query: 121 LRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQNTSSVDYSLKDGETIV 180
           LRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQNTSSVDYSLKDGETIV
Sbjct: 121 LRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQNTSSVDYSLKDGETIV 180

Query: 181 LQLKSRKSTSNVKKSTELDHVKVSSLEEEGNTKKESIFCIRPPPPPPAPLSPVASQKSPK 240
           LQLKSRKSTSNVKKSTELDHVKVSSLEEEGNTKK+SIFCIRPPPPPPAPLSPVASQKSPK
Sbjct: 181 LQLKSRKSTSNVKKSTELDHVKVSSLEEEGNTKKDSIFCIRPPPPPPAPLSPVASQKSPK 240

Query: 241 NLPPNINLEEPSKQKDTEPAKEDTNDAEVQSTLDIPDDDFGDFQTAA 287
           NLPPNINLEEPSKQKDTEPAKEDTNDAEVQSTLDIPDDDFGDFQTAA
Sbjct: 241 NLPPNINLEEPSKQKDTEPAKEDTNDAEVQSTLDIPDDDFGDFQTAA 287

BLAST of CsGy6G014850 vs. NCBI nr
Match: XP_008456431.1 (PREDICTED: uncharacterized protein At1g03900 isoform X2 [Cucumis melo] >KAA0054424.1 Adaptin ear-binding coat-associated protein 1 NECAP-1 isoform 2 [Cucumis melo var. makuwa] >TYK14612.1 Adaptin ear-binding coat-associated protein 1 NECAP-1 isoform 2 [Cucumis melo var. makuwa])

HSP 1 Score: 542 bits (1396), Expect = 8.59e-194
Identity = 276/286 (96.50%), Postives = 281/286 (98.25%), Query Frame = 0

Query: 1   MEEQQQKTPKNQEIQDDHEDNEAIELVLFQVPECYVYLIPPRKTAASYRADEWDVNKWAW 60
           MEEQQQKTPKNQEIQDDHEDNEAIELVLFQVPECYVYLIPPRKTAASYRADEWDVNKWAW
Sbjct: 1   MEEQQQKTPKNQEIQDDHEDNEAIELVLFQVPECYVYLIPPRKTAASYRADEWDVNKWAW 60

Query: 61  EGKLKVLSKGEECIIRLEDKSTGELYARAFLRDGEKHPVEPVIDSSRYFVLRVEENIDGR 120
           EGKLKVLSKGEECIIRLEDKSTGELYARAFLRDGE+HPVEPVIDSSRYFVLRVEENIDGR
Sbjct: 61  EGKLKVLSKGEECIIRLEDKSTGELYARAFLRDGEQHPVEPVIDSSRYFVLRVEENIDGR 120

Query: 121 LRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQNTSSVDYSLKDGETIV 180
           LRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQNTSSVDYSLKDGETIV
Sbjct: 121 LRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQNTSSVDYSLKDGETIV 180

Query: 181 LQLKSRKSTSNVKKSTELDHVKVSSLEEEGNTKKESIFCIRPPPPPPAPLSPVASQKSPK 240
           LQLKS+KSTSN+KKSTELDHVKVSS EEEGNTKKESIFCI+PPPPPPAPLSPVASQKSPK
Sbjct: 181 LQLKSKKSTSNIKKSTELDHVKVSSSEEEGNTKKESIFCIKPPPPPPAPLSPVASQKSPK 240

Query: 241 NLPPNINLEEPSKQKDTEPAKEDTNDAEVQSTLDIPDDDFGDFQTA 286
            LPPNINLEEPSK+KD  P KEDTNDAEVQSTLDIPDDDFGDFQTA
Sbjct: 241 YLPPNINLEEPSKKKDLVPEKEDTNDAEVQSTLDIPDDDFGDFQTA 286

BLAST of CsGy6G014850 vs. NCBI nr
Match: XP_038902341.1 (uncharacterized protein LOC120088974 [Benincasa hispida])

HSP 1 Score: 499 bits (1286), Expect = 4.25e-171
Identity = 257/286 (89.86%), Postives = 270/286 (94.41%), Query Frame = 0

Query: 1   MEEQQQKTPKNQEIQDDHEDNEAIELVLFQVPECYVYLIPPRKTAASYRADEWDVNKWAW 60
           MEE+QQK  K+QE+  DHE+NEAIELVLFQV ECYVYLIPPRK+AASYRADEWDVNKWAW
Sbjct: 402 MEEEQQKKAKDQELLVDHEENEAIELVLFQVSECYVYLIPPRKSAASYRADEWDVNKWAW 461

Query: 61  EGKLKVLSKGEECIIRLEDKSTGELYARAFLRDGEKHPVEPVIDSSRYFVLRVEENIDGR 120
           EGKLKVLSKGEECIIRLEDK+TGELYARAFLRDGE+HPVEPVIDSSRYFVLRVEENIDGR
Sbjct: 462 EGKLKVLSKGEECIIRLEDKTTGELYARAFLRDGEQHPVEPVIDSSRYFVLRVEENIDGR 521

Query: 121 LRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQNTSSVDYSLKDGETIV 180
           LRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQNTSSVDYSLKDGETIV
Sbjct: 522 LRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQNTSSVDYSLKDGETIV 581

Query: 181 LQLKSRKSTSNVKKSTELDHVKVSSLEEEGNTKKESIFCIRPPPPPPAPLSPVASQKSPK 240
           LQLKS KSTSNVKKS++LDHVK+SS  EEGNTKKESIFCI+PPPPPPAPLSPV SQKSP 
Sbjct: 582 LQLKSNKSTSNVKKSSDLDHVKISS-SEEGNTKKESIFCIKPPPPPPAPLSPVTSQKSPT 641

Query: 241 NLPPNINLEEPSKQKDTEPAKEDTNDAEVQSTLDIPDDDFGDFQTA 286
           NLP NINLEEPSK K  EPAKED+N AE QSTLD+PDDDFGDFQTA
Sbjct: 642 NLPQNINLEEPSKHKTLEPAKEDSNAAEGQSTLDVPDDDFGDFQTA 686

BLAST of CsGy6G014850 vs. NCBI nr
Match: XP_022149778.1 (uncharacterized protein At1g03900 [Momordica charantia])

HSP 1 Score: 483 bits (1243), Expect = 1.65e-170
Identity = 246/284 (86.62%), Postives = 267/284 (94.01%), Query Frame = 0

Query: 3   EQQQKTPKNQEIQDDHEDNEAIELVLFQVPECYVYLIPPRKTAASYRADEWDVNKWAWEG 62
           E+QQKT  +Q +++DHE+NEAIE+VLFQV ECYVYLIPPRK+AASYRADEWDVNKWAWEG
Sbjct: 2   EEQQKTANDQAVKEDHEENEAIEIVLFQVSECYVYLIPPRKSAASYRADEWDVNKWAWEG 61

Query: 63  KLKVLSKGEECIIRLEDKSTGELYARAFLRDGEKHPVEPVIDSSRYFVLRVEENIDGRLR 122
           KLKV+SKGEECIIRLEDKSTGELYARAFLRDGE+HPVE VIDSSRYFVLRVEENI GR R
Sbjct: 62  KLKVVSKGEECIIRLEDKSTGELYARAFLRDGEQHPVETVIDSSRYFVLRVEENIGGRRR 121

Query: 123 HAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQNTSSVDYSLKDGETIVLQ 182
           HAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQNTSSVDYSLKDGETIVLQ
Sbjct: 122 HAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQNTSSVDYSLKDGETIVLQ 181

Query: 183 LKSRKSTSNVKKSTELDHVKVSSLEEEGNTKKESIFCIRPPPPPPAPLSPVASQKSPKNL 242
           LKS+KSTSN+KK +ELD VKVSS EE+G+TKKESIFCI+PPPPP +PLSPV +QKSP NL
Sbjct: 182 LKSKKSTSNIKKCSELD-VKVSSSEEDGSTKKESIFCIKPPPPPLSPLSPVEAQKSPTNL 241

Query: 243 PPNINLEEPSKQKDTEPAKEDTNDAEVQSTLDIPDDDFGDFQTA 286
           PPNINLEEPSK K +EPAKED+N AE QSTLD+PDDDFGDFQTA
Sbjct: 242 PPNINLEEPSKHKTSEPAKEDSNPAEAQSTLDVPDDDFGDFQTA 284

BLAST of CsGy6G014850 vs. NCBI nr
Match: KAG6570537.1 (Adaptin ear-binding coat-associated protein 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 483 bits (1243), Expect = 1.71e-170
Identity = 248/286 (86.71%), Postives = 268/286 (93.71%), Query Frame = 0

Query: 1   MEEQQQKTPKNQEIQDDHEDNEAIELVLFQVPECYVYLIPPRKTAASYRADEWDVNKWAW 60
           MEE+QQKT K+QE Q+DHE+NEAIELVLFQV ECYVYLIPPRK+AASYRADEWDVNKWAW
Sbjct: 1   MEEKQQKTAKDQERQEDHEENEAIELVLFQVSECYVYLIPPRKSAASYRADEWDVNKWAW 60

Query: 61  EGKLKVLSKGEECIIRLEDKSTGELYARAFLRDGEKHPVEPVIDSSRYFVLRVEENIDGR 120
           EGKLKVLSKGEECIIRLEDK+TGELYARAFLR+GE+HPVE VIDSSRYFVLR+EENI GR
Sbjct: 61  EGKLKVLSKGEECIIRLEDKATGELYARAFLREGEQHPVETVIDSSRYFVLRIEENIGGR 120

Query: 121 LRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQNTSSVDYSLKDGETIV 180
           LRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQ TSSVDYSLKDGETIV
Sbjct: 121 LRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQKTSSVDYSLKDGETIV 180

Query: 181 LQLKSRKSTSNVKKSTELDHVKVSSLEEEGNTKKESIFCIRPPPPPPAPLSPVASQKSPK 240
           LQ KS+KSTSN+KK +ELD VKVS  EEEGNTKKESIFCI+PPPPPPA LSPVA+QKSP 
Sbjct: 181 LQFKSKKSTSNIKKPSELD-VKVSPPEEEGNTKKESIFCIKPPPPPPALLSPVAAQKSPT 240

Query: 241 NLPPNINLEEPSKQKDTEPAKEDTNDAEVQSTLDIPDDDFGDFQTA 286
           N+PPNINL++PSK K  EPAKED+NDAE QSTLD+PDDDFG+FQ A
Sbjct: 241 NVPPNINLDDPSKHKLAEPAKEDSNDAEGQSTLDLPDDDFGNFQAA 285

BLAST of CsGy6G014850 vs. ExPASy TrEMBL
Match: A0A0A0KHL0 (DUF1681 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G239700 PE=4 SV=1)

HSP 1 Score: 560 bits (1444), Expect = 2.00e-201
Identity = 286/287 (99.65%), Postives = 287/287 (100.00%), Query Frame = 0

Query: 1   MEEQQQKTPKNQEIQDDHEDNEAIELVLFQVPECYVYLIPPRKTAASYRADEWDVNKWAW 60
           MEEQQQKTPKNQEIQDDHEDNEAIELVLFQVPECYVYLIPPRKTAASYRADEWDVNKWAW
Sbjct: 1   MEEQQQKTPKNQEIQDDHEDNEAIELVLFQVPECYVYLIPPRKTAASYRADEWDVNKWAW 60

Query: 61  EGKLKVLSKGEECIIRLEDKSTGELYARAFLRDGEKHPVEPVIDSSRYFVLRVEENIDGR 120
           EGKLKVLSKGEECIIRLEDKSTGELYARAFLRDGEKHPVEPVIDSSRYFVLRVEENIDGR
Sbjct: 61  EGKLKVLSKGEECIIRLEDKSTGELYARAFLRDGEKHPVEPVIDSSRYFVLRVEENIDGR 120

Query: 121 LRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQNTSSVDYSLKDGETIV 180
           LRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQNTSSVDYSLKDGETIV
Sbjct: 121 LRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQNTSSVDYSLKDGETIV 180

Query: 181 LQLKSRKSTSNVKKSTELDHVKVSSLEEEGNTKKESIFCIRPPPPPPAPLSPVASQKSPK 240
           LQLKSRKSTSNVKKSTELDHVKVSSLEEEGNTKK+SIFCIRPPPPPPAPLSPVASQKSPK
Sbjct: 181 LQLKSRKSTSNVKKSTELDHVKVSSLEEEGNTKKDSIFCIRPPPPPPAPLSPVASQKSPK 240

Query: 241 NLPPNINLEEPSKQKDTEPAKEDTNDAEVQSTLDIPDDDFGDFQTAA 287
           NLPPNINLEEPSKQKDTEPAKEDTNDAEVQSTLDIPDDDFGDFQTAA
Sbjct: 241 NLPPNINLEEPSKQKDTEPAKEDTNDAEVQSTLDIPDDDFGDFQTAA 287

BLAST of CsGy6G014850 vs. ExPASy TrEMBL
Match: A0A5A7UGZ1 (Adaptin ear-binding coat-associated protein 1 NECAP-1 isoform 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold552G001230 PE=4 SV=1)

HSP 1 Score: 542 bits (1396), Expect = 4.16e-194
Identity = 276/286 (96.50%), Postives = 281/286 (98.25%), Query Frame = 0

Query: 1   MEEQQQKTPKNQEIQDDHEDNEAIELVLFQVPECYVYLIPPRKTAASYRADEWDVNKWAW 60
           MEEQQQKTPKNQEIQDDHEDNEAIELVLFQVPECYVYLIPPRKTAASYRADEWDVNKWAW
Sbjct: 1   MEEQQQKTPKNQEIQDDHEDNEAIELVLFQVPECYVYLIPPRKTAASYRADEWDVNKWAW 60

Query: 61  EGKLKVLSKGEECIIRLEDKSTGELYARAFLRDGEKHPVEPVIDSSRYFVLRVEENIDGR 120
           EGKLKVLSKGEECIIRLEDKSTGELYARAFLRDGE+HPVEPVIDSSRYFVLRVEENIDGR
Sbjct: 61  EGKLKVLSKGEECIIRLEDKSTGELYARAFLRDGEQHPVEPVIDSSRYFVLRVEENIDGR 120

Query: 121 LRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQNTSSVDYSLKDGETIV 180
           LRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQNTSSVDYSLKDGETIV
Sbjct: 121 LRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQNTSSVDYSLKDGETIV 180

Query: 181 LQLKSRKSTSNVKKSTELDHVKVSSLEEEGNTKKESIFCIRPPPPPPAPLSPVASQKSPK 240
           LQLKS+KSTSN+KKSTELDHVKVSS EEEGNTKKESIFCI+PPPPPPAPLSPVASQKSPK
Sbjct: 181 LQLKSKKSTSNIKKSTELDHVKVSSSEEEGNTKKESIFCIKPPPPPPAPLSPVASQKSPK 240

Query: 241 NLPPNINLEEPSKQKDTEPAKEDTNDAEVQSTLDIPDDDFGDFQTA 286
            LPPNINLEEPSK+KD  P KEDTNDAEVQSTLDIPDDDFGDFQTA
Sbjct: 241 YLPPNINLEEPSKKKDLVPEKEDTNDAEVQSTLDIPDDDFGDFQTA 286

BLAST of CsGy6G014850 vs. ExPASy TrEMBL
Match: A0A1S3C3X0 (uncharacterized protein At1g03900 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103496372 PE=4 SV=1)

HSP 1 Score: 542 bits (1396), Expect = 4.16e-194
Identity = 276/286 (96.50%), Postives = 281/286 (98.25%), Query Frame = 0

Query: 1   MEEQQQKTPKNQEIQDDHEDNEAIELVLFQVPECYVYLIPPRKTAASYRADEWDVNKWAW 60
           MEEQQQKTPKNQEIQDDHEDNEAIELVLFQVPECYVYLIPPRKTAASYRADEWDVNKWAW
Sbjct: 1   MEEQQQKTPKNQEIQDDHEDNEAIELVLFQVPECYVYLIPPRKTAASYRADEWDVNKWAW 60

Query: 61  EGKLKVLSKGEECIIRLEDKSTGELYARAFLRDGEKHPVEPVIDSSRYFVLRVEENIDGR 120
           EGKLKVLSKGEECIIRLEDKSTGELYARAFLRDGE+HPVEPVIDSSRYFVLRVEENIDGR
Sbjct: 61  EGKLKVLSKGEECIIRLEDKSTGELYARAFLRDGEQHPVEPVIDSSRYFVLRVEENIDGR 120

Query: 121 LRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQNTSSVDYSLKDGETIV 180
           LRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQNTSSVDYSLKDGETIV
Sbjct: 121 LRHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQNTSSVDYSLKDGETIV 180

Query: 181 LQLKSRKSTSNVKKSTELDHVKVSSLEEEGNTKKESIFCIRPPPPPPAPLSPVASQKSPK 240
           LQLKS+KSTSN+KKSTELDHVKVSS EEEGNTKKESIFCI+PPPPPPAPLSPVASQKSPK
Sbjct: 181 LQLKSKKSTSNIKKSTELDHVKVSSSEEEGNTKKESIFCIKPPPPPPAPLSPVASQKSPK 240

Query: 241 NLPPNINLEEPSKQKDTEPAKEDTNDAEVQSTLDIPDDDFGDFQTA 286
            LPPNINLEEPSK+KD  P KEDTNDAEVQSTLDIPDDDFGDFQTA
Sbjct: 241 YLPPNINLEEPSKKKDLVPEKEDTNDAEVQSTLDIPDDDFGDFQTA 286

BLAST of CsGy6G014850 vs. ExPASy TrEMBL
Match: A0A6J1D7P3 (uncharacterized protein At1g03900 OS=Momordica charantia OX=3673 GN=LOC111018125 PE=4 SV=1)

HSP 1 Score: 483 bits (1243), Expect = 7.97e-171
Identity = 246/284 (86.62%), Postives = 267/284 (94.01%), Query Frame = 0

Query: 3   EQQQKTPKNQEIQDDHEDNEAIELVLFQVPECYVYLIPPRKTAASYRADEWDVNKWAWEG 62
           E+QQKT  +Q +++DHE+NEAIE+VLFQV ECYVYLIPPRK+AASYRADEWDVNKWAWEG
Sbjct: 2   EEQQKTANDQAVKEDHEENEAIEIVLFQVSECYVYLIPPRKSAASYRADEWDVNKWAWEG 61

Query: 63  KLKVLSKGEECIIRLEDKSTGELYARAFLRDGEKHPVEPVIDSSRYFVLRVEENIDGRLR 122
           KLKV+SKGEECIIRLEDKSTGELYARAFLRDGE+HPVE VIDSSRYFVLRVEENI GR R
Sbjct: 62  KLKVVSKGEECIIRLEDKSTGELYARAFLRDGEQHPVETVIDSSRYFVLRVEENIGGRRR 121

Query: 123 HAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQNTSSVDYSLKDGETIVLQ 182
           HAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQNTSSVDYSLKDGETIVLQ
Sbjct: 122 HAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQNTSSVDYSLKDGETIVLQ 181

Query: 183 LKSRKSTSNVKKSTELDHVKVSSLEEEGNTKKESIFCIRPPPPPPAPLSPVASQKSPKNL 242
           LKS+KSTSN+KK +ELD VKVSS EE+G+TKKESIFCI+PPPPP +PLSPV +QKSP NL
Sbjct: 182 LKSKKSTSNIKKCSELD-VKVSSSEEDGSTKKESIFCIKPPPPPLSPLSPVEAQKSPTNL 241

Query: 243 PPNINLEEPSKQKDTEPAKEDTNDAEVQSTLDIPDDDFGDFQTA 286
           PPNINLEEPSK K +EPAKED+N AE QSTLD+PDDDFGDFQTA
Sbjct: 242 PPNINLEEPSKHKTSEPAKEDSNPAEAQSTLDVPDDDFGDFQTA 284

BLAST of CsGy6G014850 vs. ExPASy TrEMBL
Match: A0A6J1FX33 (uncharacterized protein At1g03900-like OS=Cucurbita moschata OX=3662 GN=LOC111449048 PE=4 SV=1)

HSP 1 Score: 480 bits (1236), Expect = 9.99e-170
Identity = 245/285 (85.96%), Postives = 268/285 (94.04%), Query Frame = 0

Query: 2   EEQQQKTPKNQEIQDDHEDNEAIELVLFQVPECYVYLIPPRKTAASYRADEWDVNKWAWE 61
           EE+QQKT K+Q++Q+DHE+NEAIELVLFQV ECYVYLIPPRK+AASYRADEWDVNKWAWE
Sbjct: 3   EEKQQKTAKDQKLQEDHEENEAIELVLFQVSECYVYLIPPRKSAASYRADEWDVNKWAWE 62

Query: 62  GKLKVLSKGEECIIRLEDKSTGELYARAFLRDGEKHPVEPVIDSSRYFVLRVEENIDGRL 121
           GKLKVLSKGEECIIRLEDK+TGELYARAFLR+GE+HPVE VIDSSRYFVLR+EENI GRL
Sbjct: 63  GKLKVLSKGEECIIRLEDKATGELYARAFLREGEQHPVETVIDSSRYFVLRIEENIGGRL 122

Query: 122 RHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQNTSSVDYSLKDGETIVL 181
           RHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQ TSSVDYSLKDGETIVL
Sbjct: 123 RHAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQKTSSVDYSLKDGETIVL 182

Query: 182 QLKSRKSTSNVKKSTELDHVKVSSLEEEGNTKKESIFCIRPPPPPPAPLSPVASQKSPKN 241
           Q KS+KSTSN+KK +ELD VKVS  EEEGNTKKESIFCI+PPPPPPA LSPVA+QKSP N
Sbjct: 183 QFKSKKSTSNIKKPSELD-VKVSPPEEEGNTKKESIFCIKPPPPPPALLSPVAAQKSPTN 242

Query: 242 LPPNINLEEPSKQKDTEPAKEDTNDAEVQSTLDIPDDDFGDFQTA 286
           +PPNINL++PSK K  EPA+ED+NDAE QSTLD+PDDDFG+FQ A
Sbjct: 243 VPPNINLDDPSKHKIAEPAREDSNDAEGQSTLDLPDDDFGNFQAA 286

BLAST of CsGy6G014850 vs. TAIR 10
Match: AT3G58600.1 (Adaptin ear-binding coat-associated protein 1 NECAP-1 )

HSP 1 Score: 362.1 bits (928), Expect = 4.2e-100
Identity = 193/292 (66.10%), Postives = 232/292 (79.45%), Query Frame = 0

Query: 3   EQQQKTPKNQEIQDDHEDNEAIELVLFQVPECYVYLIPPRKTAASYRADEWDVNKWAWEG 62
           E+ +K+  +    DD+E+ EAIE+ LFQVPECYVYLIPPRKTAASYRADEWDVNKWAWEG
Sbjct: 11  EEDKKSSTDPVNVDDNEEREAIEIALFQVPECYVYLIPPRKTAASYRADEWDVNKWAWEG 70

Query: 63  KLKVLSKGEECIIRLEDKSTGELYARAFLRDGEKHPVEPVIDSSRYFVLRVEENIDGRLR 122
            LKV+SKGEECII+L DK+TGELYA+AFLR+GE HPVE VIDSSRYFVLRVEE IDGR+R
Sbjct: 71  ALKVVSKGEECIIKLVDKTTGELYAQAFLREGELHPVEAVIDSSRYFVLRVEEKIDGRVR 130

Query: 123 HAFIGIGFRERTEAYDFQAALHDHMKYLNKKKSAEEMEQQFQNTSSVDYSLKDGETIVLQ 182
           HAFIG+GFRERTEAYDFQAALHDHMKYLNKKK+AEEMEQ +QNTSSVDYSLK+GETIVLQ
Sbjct: 131 HAFIGLGFRERTEAYDFQAALHDHMKYLNKKKTAEEMEQHYQNTSSVDYSLKEGETIVLQ 190

Query: 183 LKSRKSTSNVKKSTELDHVKVSSLEEEGNTKKESIFCIRPPPPPPAPLSPV-ASQKSPKN 242
           LK+R    +  K+ E     + SLE++G + + +I  I  PPPPP PLSPV  +QKSP +
Sbjct: 191 LKNRSDKDSKSKTVEKSLGNL-SLEDKGKSIETTIPSIILPPPPPGPLSPVTTAQKSPSS 250

Query: 243 LPPNINLEEPSKQKDTEPAKEDTND-------AEVQSTLDIPDDDFGDFQTA 287
           LPP+++L+  S+Q+D +  +E+  +       A+     D PDDDFGDFQ A
Sbjct: 251 LPPSLSLQRSSEQQDLDTKREEEEEEKKEDLKAKDGGVEDAPDDDFGDFQAA 301

BLAST of CsGy6G014850 vs. TAIR 10
Match: AT1G03900.1 (non-intrinsic ABC protein 4 )

HSP 1 Score: 146.7 bits (369), Expect = 2.8e-35
Identity = 90/235 (38.30%), Postives = 131/235 (55.74%), Query Frame = 0

Query: 16  DDHEDNEAIELVLFQVPECYVYLIPPRKTAASYRADEWDVNKWAWEGKLKVLSKGEECII 75
           ++ E+ E  E  L  V E  VY IPPR T+  Y+  EW  +   W G+L+V+S  + C I
Sbjct: 4   EEEEEEETFEHTLLVVREVSVYKIPPRTTSGGYKCGEWLQSDKIWSGRLRVVSCKDRCEI 63

Query: 76  RLEDKSTGELYARAFLRDGEK-HPVEPVIDSSRYFVLRVEENIDGRLRHAFIGIGFRERT 135
           RLED ++G+L+A  F+  G + + VEP +DSSRYFVLR++   DGR ++AFIG+GF ER 
Sbjct: 64  RLEDSNSGDLFAACFVDPGRRENSVEPSLDSSRYFVLRID---DGRGKYAFIGLGFAERN 123

Query: 136 EAYDFQAALHDHMKYLNKKKSAE-----EMEQQFQNTSSVDYSLKDGETIVLQLKSRKST 195
           EA+DF  AL DH KY+ ++K  E     E +       +V++ LK+GETI + +K + +T
Sbjct: 124 EAFDFNVALSDHEKYVRREKEKETGETSESDNHIDIHPAVNHRLKEGETIRINVKPKPTT 183

Query: 196 SNVKKSTELDHVKVSSLEEEGNTKKESIFCIRPP--------PPPPAPLSPVASQ 237
           +            + S    G  K + +    PP        P PP P  PVAS+
Sbjct: 184 NG---------TGMLSAALSGTGKPKPLALAPPPKAAGVTRSPLPPPPNDPVASR 226

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q681Q73.9e-3438.30Uncharacterized protein At1g03900 OS=Arabidopsis thaliana OX=3702 GN=At1g03900 P... [more]
Q6P7562.6e-3041.72Adaptin ear-binding coat-associated protein 2 OS=Rattus norvegicus OX=10116 GN=N... [more]
Q9NVZ31.0e-2937.38Adaptin ear-binding coat-associated protein 2 OS=Homo sapiens OX=9606 GN=NECAP2 ... [more]
Q5E9Q41.3e-2936.92Adaptin ear-binding coat-associated protein 2 OS=Bos taurus OX=9913 GN=NECAP2 PE... [more]
Q9CR954.2e-2831.90Adaptin ear-binding coat-associated protein 1 OS=Mus musculus OX=10090 GN=Necap1... [more]
Match NameE-valueIdentityDescription
XP_004150324.14.14e-20199.65uncharacterized protein At1g03900 [Cucumis sativus] >KGN47271.1 hypothetical pro... [more]
XP_008456431.18.59e-19496.50PREDICTED: uncharacterized protein At1g03900 isoform X2 [Cucumis melo] >KAA00544... [more]
XP_038902341.14.25e-17189.86uncharacterized protein LOC120088974 [Benincasa hispida][more]
XP_022149778.11.65e-17086.62uncharacterized protein At1g03900 [Momordica charantia][more]
KAG6570537.11.71e-17086.71Adaptin ear-binding coat-associated protein 1, partial [Cucurbita argyrosperma s... [more]
Match NameE-valueIdentityDescription
A0A0A0KHL02.00e-20199.65DUF1681 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G239700 PE=... [more]
A0A5A7UGZ14.16e-19496.50Adaptin ear-binding coat-associated protein 1 NECAP-1 isoform 2 OS=Cucumis melo ... [more]
A0A1S3C3X04.16e-19496.50uncharacterized protein At1g03900 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10349... [more]
A0A6J1D7P37.97e-17186.62uncharacterized protein At1g03900 OS=Momordica charantia OX=3673 GN=LOC111018125... [more]
A0A6J1FX339.99e-17085.96uncharacterized protein At1g03900-like OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
Match NameE-valueIdentityDescription
AT3G58600.14.2e-10066.10Adaptin ear-binding coat-associated protein 1 NECAP-1 [more]
AT1G03900.12.8e-3538.30non-intrinsic ABC protein 4 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012466NECAP, PHear domainPFAMPF07933DUF1681coord: 24..183
e-value: 8.4E-52
score: 174.9
IPR012466NECAP, PHear domainCDDcd13228PHear_NECAPcoord: 27..148
e-value: 1.69112E-71
score: 213.956
IPR011993PH-like domain superfamilyGENE3D2.30.29.30coord: 28..153
e-value: 5.9E-45
score: 154.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 219..233
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 234..250
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 204..287
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 251..268
NoneNo IPR availablePANTHERPTHR12847ATP-BINDING CASSETTE ABC TRANSPORTER-RELATEDcoord: 5..286
NoneNo IPR availablePANTHERPTHR12847:SF9ADAPTIN EAR-BINDING COAT-ASSOCIATED PROTEIN 1 NECAP-1coord: 5..286
NoneNo IPR availableSUPERFAMILY50729PH domain-likecoord: 21..152

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy6G014850.1CsGy6G014850.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006897 endocytosis
cellular_component GO:0030125 clathrin vesicle coat
cellular_component GO:0016020 membrane