CsGy6G010530 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy6G010530
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionMannan endo-1,4-beta-mannosidase
LocationGy14Chr6: 9043879 .. 9048022 (+)
RNA-Seq ExpressionCsGy6G010530
SyntenyCsGy6G010530
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACATAGCTTTAAGAAAATGGAAATGAAGATGGGAGACAAGGGAAAACTCTAACATGTGAGTGATTCAGAAGAAGTTTATTTAAATCCAGTGTAGAAGTAAGATGATCTTTGTCTGTTTACTAAAAGAAAATTGTGGGGGTTTGAAAGATTTTAACGAGAAAATGGCAAAAAAAAAAAAAAACGTAGTTAAGAAAAAGATGAATATTTTAATCTCTGCTGGTTTTTGTTGAAAATTTGAAAATAACCCATTTACGTGTGTTGAACAAGACTGTTGCTTTTTGGGGTCGTGAGAGAAAAGATGGAAAGAAAATAAGAGGGTCTGTAGATCTAATTAAACCATCAAAAGATACCAACATTCTACCATACTCTCTCAACCTCTTTTTTCTTCTTTTGCTTAATCTCTCATTAATTTTTGGGCGACCAGTTTTGGTGTGATTTCTTCAATCTTATAAATTCATCAAAACCAATGATTGATTGCAATTAGCTTCAACTCCGAGTATTATTAGTATTCAATCCTTCACGAACAATCGTTGGCGTACATTTGTGGTAGAAATAGGGATCTGGGTATACCATAACATCGTCTGATTTTGCCGACATTCTTGTTCTTTGTGGTTTATTGTTGTAAAATCGCATCTGGGTACGAGTGGTTCTTTGATTTTCTGTGATAGATTGCTATAAACATTGAGTTTTTTTTTTTTTTACAATCATTTCGATTTGCGTTTTGGGTTTTCAATCTCCCTGTTTGTAGTGATGGCGGGGAATGGGTTGTTTTACCCGATTCTTGGATTCGCATCCTGTTTGGCTTTCATTTACTTGTCGTTTGGAGATTTGAGGATTGGGGACACTAAAGTAATCCCCATAGTAGATTTTGTAGAGAGGAACGGAACGCAGTTCATGTTGAACGGGAAGGCGTTTTATATTAATGGATGGAATTCTTATTGGTTTATGGACCATTCCGTTGAGGAATATAGAAAGCCTAGAATCAGAGCCATGCTGCAGGCGGCGAGAAAGATGGGGCTTACTGTTTGCCGGACTTGGGCTTTCAATGATGGTGGTTACAATGCCCTTCAGGTTTCTCCCGGAAGGTTTAACGAGCGAGTCTTTAAGGTATTGATTGAGCTGCATTTTCTGAGATTTCTGCTGTTTCTGTGATTGGATTCTTCATTTATTTGATTCTACTCTTAATGCTTTGGTTTTGTGTTCGTAATTTGTTTCTTTTTCTGAAATTTTATGGCAATGTCTGTTTTAGAGAGAGATGAGTGATTCTGCAGGGTTTGAAAGGTTCCATTGGCCAAAGAATGCAAAGCTTGGATGCTTCCACCGACAGAAAAACTGTGTCGGTAGTCATAAAACAATATGGAATTATTTTGTGAATCGCATGATTTTCTTGGAAAAGAAACGGTAGTTCTTGCCATTATGGTTAAGATGAATGAATTAGACTGAAGACAATAAATTATCTGTCAGTTCCTTTTGGCATTTAAAGGTGGATTAATTGAGTATGATTTAAATTCTCATGTTCTGTAACCCCAATAAGAAGCATTATAACTTGTATTTCTGTGGAAGATCAATCTGGTTTTGTTTGTTATCTTGTCAATATAGTTTCAAGTTCAAAAAATGAAGACTGGAATTCATGGAAGAACCAGAAAACTTTCTATTTCCATCTATCTCGCTTTCTCTTTTTGAGAAATATAAGGATAAATGACACATTATCCAAAAAATACACAAGAAAGCATATGTTTGTATATATTTTTTCCATAGCAATTGTTTAGTTTGATGGCGTTTTTTATTTGCGCAAATGCATATGTGAAGTTGTGCTGTTATCAGCTTACGTTTCAATTGGTCCTTCCTGGTGGCAGGCTTTGGATCATGTCATTGCAGAATCAAGGCAGCATGGGATAAGACTTCTTCTTAGCTTAGTTAACAACTTGCAAGCATACGGTGGGAAGACTCAGTATGTGAAGTGGGCATGGCAAGATGGTGTTGGTTTAAGCTCTTCCAATGATTCCTTCTTTTATGATCCATCAATACGCATTTATTTTAAAAATTATCTCAAGGTACATTCCTTATTTATCAATGAGTTTGTCCAATATGTTTCATAACATTGATGATTTGGTATGCATCCTGAGCTATGCCATGGATTCAAATATATTGATCCAATTGCTATTTACCATTGCATAAGCCATTAGTTCTCGGGTTCAAACAAAAATTCTTACCCTTGTCTTTGCAGATCTTACATTGCTAAATTCTACCTTATGGTGTTTTAGTGGTTCTAATGTGCACTATATTTTTCACTTTGTCAGACCGTCCTGACAAGAAAGAATTCTATCACTGGAATTGAATATAGAAATGATCCCACCATCTTTGGATGGGAATTGATTAATGAGCCACGTTGCATGACCGATGCTTCAGGCGATACACTTCAAGTAAGTTTTAAGATTTTGGTTCTTCTGATTTCTGAATGCTGAACCAAATGAGAATTGAAATAAATGGATTCTGGTGTACTCCCCAATTAAGTTGTAAGATTTTGATTCTTCTTGCTTCTAAATGTTAAACCAAAGGAAAACTAACCCTCATTGAACTTACTGTACTCCCGTTTGAGTCTTTTGATAGCTCACTTTTGTAATTTGGGATTGTTACGCAACTTTCTTTCTTCCAAACTTTTTCTTGTGAAAGTTATGAGCTCTCTACTTGGTATTTCTCAACCTCAACTTTGCTTCAAGTTGATTTCTCTAAAGCAAAGTTGCGTTTATTTTTCACCATGTTTTAAGTGTATTTTACCAGAATTAACTCAACGTCTAAAACGAACCTACCAGTCCCAATGTGGAATATATCTTTATCAGTTAATGGCAGAAGCAATATTCACAAATGGACTCATTTTGACCTTGTGTTTGATCCTAATATATGTTTCTCAAGATTTTTGTAAGTATGACCCAATCCTATAGACACTAATGTAGAATGACCCAATCCTAAATAATCAATGAAAACTAACCTTCAGTTGACGTCACATAGCACACTATCGCATTAGAGACATCCCTCCATTCGCAAAATAGTCGGTGCCCTAATGCAATTCATTTCAATAGCAATGTTTCTTTTTAATCAACAGTTGTCCAATCAGCAATGATCTAAAGAAACATCATATTGAAATGAATATCGGTGACTATTTTGCAATGGGTAAGACGTCTTGTTGCAAAAAGAAGTTTCGAGCAGTAGAGTGCTTTGTGATGTTAGTTGAAGGTTCGTTTTCATTGGTTTTTAGGATAGAGTCATTTCACATCAATGCTGCCACCAGGATTGGGTCATCAAATCTCTTGTTTCTCTTCCCACCCCCCACCCCCAAGGGAAGAAAAGAAAAAAGCATCGTTTCTATATCTATGCATAAGCTTATTTTGAGGAATAAAATTTGTAGGAATGGATTGAAGAAATGACAGCTTACATTAAATCGATTGACAAGAAACATCTGCTGACTGTTGGGTTGGAAGGATTTTATGGTCCAAATAGCCCGAAGAAATCTACTGTGAACCCGGAAGAGTGGGCATCAAGACTTGGATCGGATTTCATCCGCAATTCTGAGATCCAACACGTGGATTTTGCCTCTGTTCATATCTACCCCGACCATTGGTAAGCATTGATGAATCTCAGTTTAAAACTTGGATGGCAATCGTCAATGATTCTACTCATGTCCATGCTCTTAAACCTGTTATTTACATAACCACATTAACATTCTGTGTCGATTAGGTTCCACGATCAAGATTTCGAAGATGAACTGAAATTTGTCTCCAAGTGGATGCTCTCCCATATTGAAGATGGTGACAAAGAGTTGAAGAAGCCAGTCATGTTTACTGAATTTGGACTGTCAGATCTGAACAAGGGCTTCACCCCAGCTCAACGGGATAGTTTTTACAAAACCGTTTACGACGTCATTTACAAATCTGCCAAGAGGAATAGATCAGGAGCTGGCTCCTTAGCATGGCAGTTCCTTGTGGAAGGAATGGAAGAGAGTAATGATGATTTCGGGATCGTCCCGTGGGAAAGATCGTCGATATATCAGCTTATTATAGAGCAATCATGCAGGTTGGCAAGAATTGGTGGAGATACCCAACAGCTAAAAGCATTGAAGTATGTGTGTGCTCAGAGATGGTGA

mRNA sequence

ATGACATAGCTTTAAGAAAATGGAAATGAAGATGGGAGACAAGGGAAAACTCTAACATATTTTGTAGAGAGGAACGGAACGCAGTTCATGTTGAACGGGAAGGCGTTTTATATTAATGGATGGAATTCTTATTGGTTTATGGACCATTCCGTTGAGGAATATAGAAAGCCTAGAATCAGAGCCATGCTGCAGGCGGCGAGAAAGATGGGGCTTACTGTTTGCCGGACTTGGGCTTTCAATGATGGTGGTTACAATGCCCTTCAGGTTTCTCCCGGAAGGTTTAACGAGCGAGTCTTTAAGGCTTTGGATCATGTCATTGCAGAATCAAGGCAGCATGGGATAAGACTTCTTCTTAGCTTAGTTAACAACTTGCAAGCATACGGTGGGAAGACTCAGTATGTGAAGTGGGCATGGCAAGATGGTGTTGGTTTAAGCTCTTCCAATGATTCCTTCTTTTATGATCCATCAATACGCATTTATTTTAAAAATTATCTCAAGACCGTCCTGACAAGAAAGAATTCTATCACTGGAATTGAATATAGAAATGATCCCACCATCTTTGGATGGGAATTGATTAATGAGCCACGTTGCATGACCGATGCTTCAGGCGATACACTTCAAGAATGGATTGAAGAAATGACAGCTTACATTAAATCGATTGACAAGAAACATCTGCTGACTGTTGGGTTGGAAGGATTTTATGGTCCAAATAGCCCGAAGAAATCTACTGTGAACCCGGAAGAGTGGGCATCAAGACTTGGATCGGATTTCATCCGCAATTCTGAGATCCAACACGTGGATTTTGCCTCTGTTCATATCTACCCCGACCATTGGTTCCACGATCAAGATTTCGAAGATGAACTGAAATTTGTCTCCAAGTGGATGCTCTCCCATATTGAAGATGGTGACAAAGAGTTGAAGAAGCCAGTCATGTTTACTGAATTTGGACTGTCAGATCTGAACAAGGGCTTCACCCCAGCTCAACGGGATAGTTTTTACAAAACCGTTTACGACGTCATTTACAAATCTGCCAAGAGGAATAGATCAGGAGCTGGCTCCTTAGCATGGCAGTTCCTTGTGGAAGGAATGGAAGAGAGTAATGATGATTTCGGGATCGTCCCGTGGGAAAGATCGTCGATATATCAGCTTATTATAGAGCAATCATGCAGGTTGGCAAGAATTGGTGGAGATACCCAACAGCTAAAAGCATTGAAGTATGTGTGTGCTCAGAGATGGTGA

Coding sequence (CDS)

ATGTTGAACGGGAAGGCGTTTTATATTAATGGATGGAATTCTTATTGGTTTATGGACCATTCCGTTGAGGAATATAGAAAGCCTAGAATCAGAGCCATGCTGCAGGCGGCGAGAAAGATGGGGCTTACTGTTTGCCGGACTTGGGCTTTCAATGATGGTGGTTACAATGCCCTTCAGGTTTCTCCCGGAAGGTTTAACGAGCGAGTCTTTAAGGCTTTGGATCATGTCATTGCAGAATCAAGGCAGCATGGGATAAGACTTCTTCTTAGCTTAGTTAACAACTTGCAAGCATACGGTGGGAAGACTCAGTATGTGAAGTGGGCATGGCAAGATGGTGTTGGTTTAAGCTCTTCCAATGATTCCTTCTTTTATGATCCATCAATACGCATTTATTTTAAAAATTATCTCAAGACCGTCCTGACAAGAAAGAATTCTATCACTGGAATTGAATATAGAAATGATCCCACCATCTTTGGATGGGAATTGATTAATGAGCCACGTTGCATGACCGATGCTTCAGGCGATACACTTCAAGAATGGATTGAAGAAATGACAGCTTACATTAAATCGATTGACAAGAAACATCTGCTGACTGTTGGGTTGGAAGGATTTTATGGTCCAAATAGCCCGAAGAAATCTACTGTGAACCCGGAAGAGTGGGCATCAAGACTTGGATCGGATTTCATCCGCAATTCTGAGATCCAACACGTGGATTTTGCCTCTGTTCATATCTACCCCGACCATTGGTTCCACGATCAAGATTTCGAAGATGAACTGAAATTTGTCTCCAAGTGGATGCTCTCCCATATTGAAGATGGTGACAAAGAGTTGAAGAAGCCAGTCATGTTTACTGAATTTGGACTGTCAGATCTGAACAAGGGCTTCACCCCAGCTCAACGGGATAGTTTTTACAAAACCGTTTACGACGTCATTTACAAATCTGCCAAGAGGAATAGATCAGGAGCTGGCTCCTTAGCATGGCAGTTCCTTGTGGAAGGAATGGAAGAGAGTAATGATGATTTCGGGATCGTCCCGTGGGAAAGATCGTCGATATATCAGCTTATTATAGAGCAATCATGCAGGTTGGCAAGAATTGGTGGAGATACCCAACAGCTAAAAGCATTGAAGTATGTGTGTGCTCAGAGATGGTGA

Protein sequence

MLNGKAFYINGWNSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVSPGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSFFYDPSIRIYFKNYLKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRDSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCRLARIGGDTQQLKALKYVCAQRW*
Homology
BLAST of CsGy6G010530 vs. ExPASy Swiss-Prot
Match: Q7Y223 (Mannan endo-1,4-beta-mannosidase 2 OS=Arabidopsis thaliana OX=3702 GN=MAN2 PE=2 SV=1)

HSP 1 Score: 649.0 bits (1673), Expect = 3.2e-185
Identity = 295/382 (77.23%), Postives = 339/382 (88.74%), Query Frame = 0

Query: 1   MLNGKAFYINGWNSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQV 60
           +++GKA Y+NGWNSYWFMDH+V ++ + R+ AML+A  KMGLTVCRTWAFNDGGYNALQ+
Sbjct: 51  VVDGKALYVNGWNSYWFMDHAVNDHSRHRVSAMLEAGAKMGLTVCRTWAFNDGGYNALQI 110

Query: 61  SPGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSND 120
           SPGRF+ERVFKALDHVIAE++ HG+RLLLSLVNNLQAYGGKTQYV WAWQ+GVGLSSSND
Sbjct: 111 SPGRFDERVFKALDHVIAEAKTHGVRLLLSLVNNLQAYGGKTQYVNWAWQEGVGLSSSND 170

Query: 121 SFFYDPSIRIYFKNYLKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEW 180
           SFF+DPSIR YFKNYL  +LTRKNS+TGIEYRNDPTIF WELINEPRCM+D SGDTLQ+W
Sbjct: 171 SFFFDPSIRRYFKNYLTVLLTRKNSLTGIEYRNDPTIFAWELINEPRCMSDVSGDTLQDW 230

Query: 181 IEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFA 240
           I EMTA+IKSID KHLLTVGLEGFYGP+SPKK TVNPE WAS LGSDF+RNS+  ++DFA
Sbjct: 231 INEMTAFIKSIDNKHLLTVGLEGFYGPSSPKKLTVNPERWASELGSDFVRNSDSPNIDFA 290

Query: 241 SVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQR 300
           SVHIYPDHWFHDQ FE++LKFV KWMLSHIEDGDKELKKPV+FTEFGLS+LNK + P+QR
Sbjct: 291 SVHIYPDHWFHDQGFEEKLKFVVKWMLSHIEDGDKELKKPVLFTEFGLSNLNKDYDPSQR 350

Query: 301 DSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSC 360
           D FY+T++DVIYKSAKR RSGAG+L WQFL+EGME  NDDFGIVPWE+ SI +L+IEQSC
Sbjct: 351 DRFYRTIFDVIYKSAKRKRSGAGTLVWQFLIEGMEGFNDDFGIVPWEQDSIQRLMIEQSC 410

Query: 361 RLARIGGDTQQLKALKYVCAQR 383
           RL+RI G     K    +C+ R
Sbjct: 411 RLSRITGRHLLDKKSIEMCSHR 432

BLAST of CsGy6G010530 vs. ExPASy Swiss-Prot
Match: Q9M0H6 (Mannan endo-1,4-beta-mannosidase 5 OS=Arabidopsis thaliana OX=3702 GN=MAN5 PE=2 SV=1)

HSP 1 Score: 619.4 bits (1596), Expect = 2.8e-176
Identity = 280/382 (73.30%), Postives = 333/382 (87.17%), Query Frame = 0

Query: 1   MLNGKAFYINGWNSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQV 60
           +++ K  Y+NGWNSYWFMDH+V+E+ +  +  ML+A  KMGLTVCRTWAFNDGGYNALQ+
Sbjct: 50  VVDDKPLYVNGWNSYWFMDHAVDEHSRNLVGEMLEAGAKMGLTVCRTWAFNDGGYNALQI 109

Query: 61  SPGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSND 120
           SPGRF+ERVF+ALDHVIAE+R+H +RLLLSLVNNLQAYGGKTQYVKWAWQ+GVGLSSSND
Sbjct: 110 SPGRFDERVFQALDHVIAEARKHDVRLLLSLVNNLQAYGGKTQYVKWAWQEGVGLSSSND 169

Query: 121 SFFYDPSIRIYFKNYLKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEW 180
           SFF+DPSIR YFKNYLK +LTRKNS+TGIEYRNDPTIF WELINEPRC TD SG TLQ+W
Sbjct: 170 SFFFDPSIRNYFKNYLKVLLTRKNSVTGIEYRNDPTIFAWELINEPRCTTDVSGKTLQDW 229

Query: 181 IEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFA 240
           I+EMT +IKSID KHLLTVGLEGFYGPNSPK  TVNPE+WAS+LG+DF++NS   ++DFA
Sbjct: 230 IDEMTGFIKSIDDKHLLTVGLEGFYGPNSPKGLTVNPEQWASQLGTDFVQNSNSSNIDFA 289

Query: 241 SVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQR 300
           SVHIYPDHWFH+Q FE++LKFV KWM SHIEDG KELKKPV+FTEFGLS+ NK + P+QR
Sbjct: 290 SVHIYPDHWFHNQTFEEKLKFVVKWMQSHIEDGLKELKKPVLFTEFGLSNQNKDYEPSQR 349

Query: 301 DSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSC 360
           D FY+ ++DV+YKSAKR +SGAG+L WQ  +EGME  NDDFGIVP E+ SIY+L+IEQSC
Sbjct: 350 DKFYRIIFDVVYKSAKRKKSGAGTLVWQLFMEGMETFNDDFGIVPHEQDSIYKLMIEQSC 409

Query: 361 RLARIGGDTQQLKALKYVCAQR 383
           RL ++ G  ++ K LK +C+ R
Sbjct: 410 RLGKVTGRLKEQK-LKELCSHR 430

BLAST of CsGy6G010530 vs. ExPASy Swiss-Prot
Match: Q0JJD4 (Mannan endo-1,4-beta-mannosidase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=MAN2 PE=2 SV=2)

HSP 1 Score: 520.8 bits (1340), Expect = 1.3e-146
Identity = 238/382 (62.30%), Postives = 301/382 (78.80%), Query Frame = 0

Query: 2   LNGKAFYINGWNSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVS 61
           L+G+ FYINGWNSYW MD +VE   +PR+ +M + A  MGLTVCRTWAFNDG YNALQ+S
Sbjct: 64  LDGRPFYINGWNSYWLMDLAVEPNTRPRVSSMFRTAVSMGLTVCRTWAFNDGSYNALQLS 123

Query: 62  PGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDS 121
           PG F+ERVFKALD V+AE+ +HG+RL+LSL NNL AYGGK QYV+WAW++GVGL++SNDS
Sbjct: 124 PGHFDERVFKALDRVVAEASEHGVRLILSLANNLDAYGGKRQYVRWAWEEGVGLTASNDS 183

Query: 122 FFYDPSIRIYFKNYLKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWI 181
           FF+DP+IR YFK YLKT+L RKN +TG+EYR+DPTI  WEL+NEPRC +D SGDTLQ W+
Sbjct: 184 FFFDPAIRDYFKVYLKTLLMRKNHLTGLEYRDDPTILAWELMNEPRCTSDPSGDTLQRWM 243

Query: 182 EEMTAYIKSIDKKHLLTVGLEGFYGP-NSPKKSTVNPEEW-ASRLGSDFIRNSEIQHVDF 241
           EEM+AY+KSIDKKHLLTVG EGFYGP +S +K  +NP EW  +  G+DFIRNS+IQ +DF
Sbjct: 244 EEMSAYVKSIDKKHLLTVGTEGFYGPTSSQEKLNINPGEWFPNNYGADFIRNSKIQDIDF 303

Query: 242 ASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQ 301
           ASVH+YPD+W      +++LKF+++W+ +H+EDGD EL+KPV+ TEFGLS   +GF  A 
Sbjct: 304 ASVHVYPDNWLQHASLDEKLKFMTRWITAHVEDGDGELEKPVLVTEFGLSHQVEGFEDAH 363

Query: 302 RDSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQS 361
           RD  Y+ VYD+++ SA+R  +  G+L WQ   EGMEE +D F IVP ER S+ +LI EQS
Sbjct: 364 RDVLYRAVYDIVHGSARRGGAAGGALVWQLAAEGMEEYHDGFSIVPSERPSMMRLIKEQS 423

Query: 362 CRLARIG-GDTQQLKALKYVCA 381
           CRLA +  G+    K LK VCA
Sbjct: 424 CRLAAVRYGEEGARKVLKTVCA 445

BLAST of CsGy6G010530 vs. ExPASy Swiss-Prot
Match: Q0DCM5 (Mannan endo-1,4-beta-mannosidase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=MAN6 PE=2 SV=2)

HSP 1 Score: 405.2 bits (1040), Expect = 8.2e-112
Identity = 190/369 (51.49%), Postives = 259/369 (70.19%), Query Frame = 0

Query: 4   GKAFYINGWNSYWFMDHSVEEYRKPRIRA-MLQAARKMGLTVCRTWAFNDGGYNALQVSP 63
           G   Y+NGWNSYW +       R P + A ML+  R+MGL+VCRTWAF+DGG  ALQ+SP
Sbjct: 67  GSPLYVNGWNSYWLL-----PARSPALAAEMLRRGRRMGLSVCRTWAFSDGGPGALQISP 126

Query: 64  GRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDSF 123
           GRF+E VF+ LD+VI E+R++ IRL+L LVNNL   GGK QYV+WA   G  +++S DSF
Sbjct: 127 GRFSEAVFQVLDYVIYEARRNHIRLILCLVNNLDNLGGKAQYVQWAQAAGANMTNSTDSF 186

Query: 124 FYDPSIRIYFKNYLKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWIE 183
           +  P+I+ Y+K+Y+K +LTR+NS + I Y ++P IF WEL+NEPRC++++SG  LQ WI 
Sbjct: 187 YSHPTIKRYYKDYVKAILTRRNSYSRIRYSDEPAIFAWELMNEPRCVSNSSGPYLQAWIA 246

Query: 184 EMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFASV 243
           EM AY+KS+D  HL+TVG EGFYGP   ++  VNP EWA+ L SDFI+NS ++H+DFASV
Sbjct: 247 EMAAYVKSLDTNHLVTVGTEGFYGPGIAERLGVNPGEWAASLCSDFIQNSAVEHIDFASV 306

Query: 244 HIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGL---SDLNKGFTPAQ 303
           H YPD W      E++++++S W+ SH+ D ++ LKKPV+FTE G    SD N   T   
Sbjct: 307 HAYPDSWLPRASLEEKVRYLSNWVDSHLNDSEQILKKPVLFTEVGYLQHSDANSNST-VD 366

Query: 304 RDSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQS 363
           RD   + VYD IY SA++ ++G+G+L WQ +VEG     D+F +V  +R S Y LI  QS
Sbjct: 367 RDIILRIVYDKIYDSARKLQAGSGALIWQLMVEGTHMYGDNFSVVARDRPSTYSLITNQS 426

Query: 364 CRLARIGGD 369
           CRL R+ G+
Sbjct: 427 CRLQRLYGE 429

BLAST of CsGy6G010530 vs. ExPASy Swiss-Prot
Match: Q9LZV3 (Mannan endo-1,4-beta-mannosidase 6 OS=Arabidopsis thaliana OX=3702 GN=MAN6 PE=2 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 2.2e-104
Identity = 173/362 (47.79%), Postives = 250/362 (69.06%), Query Frame = 0

Query: 2   LNGKAFYINGWNSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVS 61
           LNG+ FY+NG+N+YW M  + +   + ++  + Q A  +G+TV RTWAFNDG + ALQ S
Sbjct: 57  LNGQPFYVNGFNTYWMMTLAADNSTRGKVTEVFQQASAVGMTVGRTWAFNDGQWRALQKS 116

Query: 62  PGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDS 121
           P  ++E VFKALD V++E+R++ IRL+LSLVNN  AYGGK QYVKW    G+ L +S+D 
Sbjct: 117 PSVYDEEVFKALDFVLSEARKYKIRLILSLVNNWDAYGGKAQYVKWGNASGLNL-TSDDD 176

Query: 122 FFYDPSIRIYFKNYLKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWI 181
           FF +P++R +++++++TVL R N+ T I Y+NDPTIF WEL+NEPRC +D SGD LQ WI
Sbjct: 177 FFTNPTLRNFYQSHVRTVLNRVNTFTNITYKNDPTIFAWELMNEPRCPSDPSGDKLQSWI 236

Query: 182 EEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFAS 241
           +EM  ++KS+D KHL+ +GLEGFYGP++P ++  NP  +A+++G+DFIRN+++  +DFAS
Sbjct: 237 QEMAVFVKSLDAKHLVEIGLEGFYGPSAPARTRFNPNPYAAQVGTDFIRNNQVLGIDFAS 296

Query: 242 VHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRD 301
           VH+YPD W         L+F S WM +H+ED +  L  PV+FTEFG+S  + GF  + RD
Sbjct: 297 VHVYPDSWISPAVSNSFLEFTSSWMQAHVEDAEMYLGMPVLFTEFGVSAHDPGFNTSFRD 356

Query: 302 SFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCR 361
               TVY +   S ++  +GAGSL WQ   +G E  +D + +      +  ++I  QS R
Sbjct: 357 MMLNTVYKMTLNSTRKGGAGAGSLVWQVFPQGAEFMDDGYAVYLTRAHTASKIISLQSKR 416

Query: 362 LA 364
           LA
Sbjct: 417 LA 417

BLAST of CsGy6G010530 vs. NCBI nr
Match: XP_004139946.1 (mannan endo-1,4-beta-mannosidase 2 [Cucumis sativus] >KGN46738.1 hypothetical protein Csa_021081 [Cucumis sativus])

HSP 1 Score: 801 bits (2069), Expect = 2.40e-292
Identity = 383/383 (100.00%), Postives = 383/383 (100.00%), Query Frame = 0

Query: 1   MLNGKAFYINGWNSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQV 60
           MLNGKAFYINGWNSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQV
Sbjct: 49  MLNGKAFYINGWNSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQV 108

Query: 61  SPGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSND 120
           SPGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSND
Sbjct: 109 SPGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSND 168

Query: 121 SFFYDPSIRIYFKNYLKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEW 180
           SFFYDPSIRIYFKNYLKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEW
Sbjct: 169 SFFYDPSIRIYFKNYLKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEW 228

Query: 181 IEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFA 240
           IEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFA
Sbjct: 229 IEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFA 288

Query: 241 SVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQR 300
           SVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQR
Sbjct: 289 SVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQR 348

Query: 301 DSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSC 360
           DSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSC
Sbjct: 349 DSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSC 408

Query: 361 RLARIGGDTQQLKALKYVCAQRW 383
           RLARIGGDTQQLKALKYVCAQRW
Sbjct: 409 RLARIGGDTQQLKALKYVCAQRW 431

BLAST of CsGy6G010530 vs. NCBI nr
Match: XP_008448293.1 (PREDICTED: mannan endo-1,4-beta-mannosidase 2 [Cucumis melo] >TYK04966.1 mannan endo-1,4-beta-mannosidase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 792 bits (2046), Expect = 7.68e-289
Identity = 379/383 (98.96%), Postives = 380/383 (99.22%), Query Frame = 0

Query: 1   MLNGKAFYINGWNSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQV 60
           MLNGKAFYINGWNSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQV
Sbjct: 49  MLNGKAFYINGWNSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQV 108

Query: 61  SPGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSND 120
           SPGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSND
Sbjct: 109 SPGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSND 168

Query: 121 SFFYDPSIRIYFKNYLKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEW 180
           SFFYDPSIRIYFKNY+KTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEW
Sbjct: 169 SFFYDPSIRIYFKNYIKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEW 228

Query: 181 IEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFA 240
           IEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEI HVDFA
Sbjct: 229 IEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHVDFA 288

Query: 241 SVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQR 300
           SVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFT AQR
Sbjct: 289 SVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTHAQR 348

Query: 301 DSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSC 360
           DSFYKTVYDVIYKSAKR RSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSC
Sbjct: 349 DSFYKTVYDVIYKSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSC 408

Query: 361 RLARIGGDTQQLKALKYVCAQRW 383
           RLARIGGDTQQLKALKYVCAQRW
Sbjct: 409 RLARIGGDTQQLKALKYVCAQRW 431

BLAST of CsGy6G010530 vs. NCBI nr
Match: KAA0062055.1 (mannan endo-1,4-beta-mannosidase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 790 bits (2039), Expect = 8.96e-288
Identity = 377/383 (98.43%), Postives = 380/383 (99.22%), Query Frame = 0

Query: 1   MLNGKAFYINGWNSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQV 60
           +LNGKAFYINGWNSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQV
Sbjct: 49  ILNGKAFYINGWNSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQV 108

Query: 61  SPGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSND 120
           SPGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSND
Sbjct: 109 SPGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSND 168

Query: 121 SFFYDPSIRIYFKNYLKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEW 180
           SFFYDPSIRIYFKNY+KTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEW
Sbjct: 169 SFFYDPSIRIYFKNYIKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEW 228

Query: 181 IEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFA 240
           IEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEI HVDFA
Sbjct: 229 IEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHVDFA 288

Query: 241 SVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQR 300
           SVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFT AQR
Sbjct: 289 SVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTHAQR 348

Query: 301 DSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSC 360
           DSFYKTVYDVIYKSAKR RSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSC
Sbjct: 349 DSFYKTVYDVIYKSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSC 408

Query: 361 RLARIGGDTQQLKALKYVCAQRW 383
           RLA+IGGDTQQLKALKYVCAQRW
Sbjct: 409 RLAKIGGDTQQLKALKYVCAQRW 431

BLAST of CsGy6G010530 vs. NCBI nr
Match: XP_038902035.1 (mannan endo-1,4-beta-mannosidase 2-like [Benincasa hispida])

HSP 1 Score: 777 bits (2007), Expect = 6.75e-283
Identity = 371/383 (96.87%), Postives = 376/383 (98.17%), Query Frame = 0

Query: 1   MLNGKAFYINGWNSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQV 60
           MLNGKAFYINGWNSYWFMDH+VEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQV
Sbjct: 49  MLNGKAFYINGWNSYWFMDHAVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQV 108

Query: 61  SPGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSND 120
           SPGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSND
Sbjct: 109 SPGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSND 168

Query: 121 SFFYDPSIRIYFKNYLKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEW 180
           SFFYDPSIRIYFKNY+KTVLTRKNSITGIEYR+DPTIFGWELINEPRCMTDASGDTLQEW
Sbjct: 169 SFFYDPSIRIYFKNYIKTVLTRKNSITGIEYRDDPTIFGWELINEPRCMTDASGDTLQEW 228

Query: 181 IEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFA 240
           IEEMTAYIKSIDKKHLLTVGLEGFYGPNS KKSTVNPEEWASRLGSDFIRNSEI +VDFA
Sbjct: 229 IEEMTAYIKSIDKKHLLTVGLEGFYGPNSRKKSTVNPEEWASRLGSDFIRNSEIPNVDFA 288

Query: 241 SVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQR 300
           SVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGD E+KKPVMFTEFGLSDLNKGFTPAQR
Sbjct: 289 SVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDIEMKKPVMFTEFGLSDLNKGFTPAQR 348

Query: 301 DSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSC 360
           D FYKTVYDVIYKSAKR RSGAGSLAWQFLVEGMEESNDDFGIVPWER SIYQLIIEQSC
Sbjct: 349 DRFYKTVYDVIYKSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERQSIYQLIIEQSC 408

Query: 361 RLARIGGDTQQLKALKYVCAQRW 383
           RLARIGGD QQLKALKYVCAQRW
Sbjct: 409 RLARIGGDAQQLKALKYVCAQRW 431

BLAST of CsGy6G010530 vs. NCBI nr
Match: XP_023512795.1 (mannan endo-1,4-beta-mannosidase 2-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 760 bits (1962), Expect = 6.85e-277
Identity = 360/379 (94.99%), Postives = 372/379 (98.15%), Query Frame = 0

Query: 1   MLNGKAFYINGWNSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQV 60
           M+NG+AFYINGWNSYWFMDH+VE YRKPR+RAML+AARKMGLTVCRTWAF+DGGYNALQV
Sbjct: 1   MVNGRAFYINGWNSYWFMDHAVEGYRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQV 60

Query: 61  SPGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSND 120
            PGRFNE+VFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSND
Sbjct: 61  FPGRFNEQVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSND 120

Query: 121 SFFYDPSIRIYFKNYLKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEW 180
           SFFYDPSIRIYFKNY+KTVLTR+NSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEW
Sbjct: 121 SFFYDPSIRIYFKNYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEW 180

Query: 181 IEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFA 240
           IEEM+AYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEI H+DFA
Sbjct: 181 IEEMSAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHIDFA 240

Query: 241 SVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQR 300
           SVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQR
Sbjct: 241 SVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQR 300

Query: 301 DSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSC 360
           D FYKTVYDVIYKSAKR RSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIY+LIIEQSC
Sbjct: 301 DRFYKTVYDVIYKSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYRLIIEQSC 360

Query: 361 RLARIGGDTQQLKALKYVC 379
           RLARIGGD QQLKALK VC
Sbjct: 361 RLARIGGDIQQLKALKNVC 379

BLAST of CsGy6G010530 vs. ExPASy TrEMBL
Match: A0A0A0KB35 (Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G127990 PE=3 SV=1)

HSP 1 Score: 801 bits (2069), Expect = 1.16e-292
Identity = 383/383 (100.00%), Postives = 383/383 (100.00%), Query Frame = 0

Query: 1   MLNGKAFYINGWNSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQV 60
           MLNGKAFYINGWNSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQV
Sbjct: 49  MLNGKAFYINGWNSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQV 108

Query: 61  SPGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSND 120
           SPGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSND
Sbjct: 109 SPGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSND 168

Query: 121 SFFYDPSIRIYFKNYLKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEW 180
           SFFYDPSIRIYFKNYLKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEW
Sbjct: 169 SFFYDPSIRIYFKNYLKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEW 228

Query: 181 IEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFA 240
           IEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFA
Sbjct: 229 IEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFA 288

Query: 241 SVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQR 300
           SVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQR
Sbjct: 289 SVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQR 348

Query: 301 DSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSC 360
           DSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSC
Sbjct: 349 DSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSC 408

Query: 361 RLARIGGDTQQLKALKYVCAQRW 383
           RLARIGGDTQQLKALKYVCAQRW
Sbjct: 409 RLARIGGDTQQLKALKYVCAQRW 431

BLAST of CsGy6G010530 vs. ExPASy TrEMBL
Match: A0A5D3C3G0 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold143G001850 PE=3 SV=1)

HSP 1 Score: 792 bits (2046), Expect = 3.72e-289
Identity = 379/383 (98.96%), Postives = 380/383 (99.22%), Query Frame = 0

Query: 1   MLNGKAFYINGWNSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQV 60
           MLNGKAFYINGWNSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQV
Sbjct: 49  MLNGKAFYINGWNSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQV 108

Query: 61  SPGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSND 120
           SPGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSND
Sbjct: 109 SPGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSND 168

Query: 121 SFFYDPSIRIYFKNYLKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEW 180
           SFFYDPSIRIYFKNY+KTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEW
Sbjct: 169 SFFYDPSIRIYFKNYIKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEW 228

Query: 181 IEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFA 240
           IEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEI HVDFA
Sbjct: 229 IEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHVDFA 288

Query: 241 SVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQR 300
           SVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFT AQR
Sbjct: 289 SVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTHAQR 348

Query: 301 DSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSC 360
           DSFYKTVYDVIYKSAKR RSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSC
Sbjct: 349 DSFYKTVYDVIYKSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSC 408

Query: 361 RLARIGGDTQQLKALKYVCAQRW 383
           RLARIGGDTQQLKALKYVCAQRW
Sbjct: 409 RLARIGGDTQQLKALKYVCAQRW 431

BLAST of CsGy6G010530 vs. ExPASy TrEMBL
Match: A0A1S3BJC2 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103490531 PE=3 SV=1)

HSP 1 Score: 792 bits (2046), Expect = 3.72e-289
Identity = 379/383 (98.96%), Postives = 380/383 (99.22%), Query Frame = 0

Query: 1   MLNGKAFYINGWNSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQV 60
           MLNGKAFYINGWNSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQV
Sbjct: 49  MLNGKAFYINGWNSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQV 108

Query: 61  SPGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSND 120
           SPGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSND
Sbjct: 109 SPGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSND 168

Query: 121 SFFYDPSIRIYFKNYLKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEW 180
           SFFYDPSIRIYFKNY+KTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEW
Sbjct: 169 SFFYDPSIRIYFKNYIKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEW 228

Query: 181 IEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFA 240
           IEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEI HVDFA
Sbjct: 229 IEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHVDFA 288

Query: 241 SVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQR 300
           SVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFT AQR
Sbjct: 289 SVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTHAQR 348

Query: 301 DSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSC 360
           DSFYKTVYDVIYKSAKR RSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSC
Sbjct: 349 DSFYKTVYDVIYKSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSC 408

Query: 361 RLARIGGDTQQLKALKYVCAQRW 383
           RLARIGGDTQQLKALKYVCAQRW
Sbjct: 409 RLARIGGDTQQLKALKYVCAQRW 431

BLAST of CsGy6G010530 vs. ExPASy TrEMBL
Match: A0A5A7V836 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold89G003960 PE=3 SV=1)

HSP 1 Score: 790 bits (2039), Expect = 4.34e-288
Identity = 377/383 (98.43%), Postives = 380/383 (99.22%), Query Frame = 0

Query: 1   MLNGKAFYINGWNSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQV 60
           +LNGKAFYINGWNSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQV
Sbjct: 49  ILNGKAFYINGWNSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQV 108

Query: 61  SPGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSND 120
           SPGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSND
Sbjct: 109 SPGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSND 168

Query: 121 SFFYDPSIRIYFKNYLKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEW 180
           SFFYDPSIRIYFKNY+KTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEW
Sbjct: 169 SFFYDPSIRIYFKNYIKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEW 228

Query: 181 IEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFA 240
           IEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEI HVDFA
Sbjct: 229 IEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHVDFA 288

Query: 241 SVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQR 300
           SVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFT AQR
Sbjct: 289 SVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTHAQR 348

Query: 301 DSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSC 360
           DSFYKTVYDVIYKSAKR RSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSC
Sbjct: 349 DSFYKTVYDVIYKSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSC 408

Query: 361 RLARIGGDTQQLKALKYVCAQRW 383
           RLA+IGGDTQQLKALKYVCAQRW
Sbjct: 409 RLAKIGGDTQQLKALKYVCAQRW 431

BLAST of CsGy6G010530 vs. ExPASy TrEMBL
Match: A0A6J1FWH9 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111448802 PE=3 SV=1)

HSP 1 Score: 758 bits (1958), Expect = 1.35e-276
Identity = 359/379 (94.72%), Postives = 372/379 (98.15%), Query Frame = 0

Query: 1   MLNGKAFYINGWNSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQV 60
           M+NG+AFYINGWNSYWFMDH+VE YRKPR+RAML+AARKMGLTVCRTWAF+DGGYNALQV
Sbjct: 1   MVNGRAFYINGWNSYWFMDHAVEGYRKPRVRAMLRAARKMGLTVCRTWAFSDGGYNALQV 60

Query: 61  SPGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSND 120
            PGRFNE+VFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSND
Sbjct: 61  FPGRFNEQVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSND 120

Query: 121 SFFYDPSIRIYFKNYLKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEW 180
           SFFYDPSIRIYFKNY+KTVLTR+NSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEW
Sbjct: 121 SFFYDPSIRIYFKNYIKTVLTRRNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEW 180

Query: 181 IEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFA 240
           IEEM+AYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEI H+DFA
Sbjct: 181 IEEMSAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIPHIDFA 240

Query: 241 SVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQR 300
           SVHIYPDHWFHDQDFE+ELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQR
Sbjct: 241 SVHIYPDHWFHDQDFEEELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQR 300

Query: 301 DSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSC 360
           D FYKTVYDVIYKSAKR RSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIY+LIIEQSC
Sbjct: 301 DRFYKTVYDVIYKSAKRKRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYRLIIEQSC 360

Query: 361 RLARIGGDTQQLKALKYVC 379
           RLARIGGD QQLKALK VC
Sbjct: 361 RLARIGGDIQQLKALKNVC 379

BLAST of CsGy6G010530 vs. TAIR 10
Match: AT2G20680.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 649.0 bits (1673), Expect = 2.3e-186
Identity = 295/382 (77.23%), Postives = 339/382 (88.74%), Query Frame = 0

Query: 1   MLNGKAFYINGWNSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQV 60
           +++GKA Y+NGWNSYWFMDH+V ++ + R+ AML+A  KMGLTVCRTWAFNDGGYNALQ+
Sbjct: 51  VVDGKALYVNGWNSYWFMDHAVNDHSRHRVSAMLEAGAKMGLTVCRTWAFNDGGYNALQI 110

Query: 61  SPGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSND 120
           SPGRF+ERVFKALDHVIAE++ HG+RLLLSLVNNLQAYGGKTQYV WAWQ+GVGLSSSND
Sbjct: 111 SPGRFDERVFKALDHVIAEAKTHGVRLLLSLVNNLQAYGGKTQYVNWAWQEGVGLSSSND 170

Query: 121 SFFYDPSIRIYFKNYLKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEW 180
           SFF+DPSIR YFKNYL  +LTRKNS+TGIEYRNDPTIF WELINEPRCM+D SGDTLQ+W
Sbjct: 171 SFFFDPSIRRYFKNYLTVLLTRKNSLTGIEYRNDPTIFAWELINEPRCMSDVSGDTLQDW 230

Query: 181 IEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFA 240
           I EMTA+IKSID KHLLTVGLEGFYGP+SPKK TVNPE WAS LGSDF+RNS+  ++DFA
Sbjct: 231 INEMTAFIKSIDNKHLLTVGLEGFYGPSSPKKLTVNPERWASELGSDFVRNSDSPNIDFA 290

Query: 241 SVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQR 300
           SVHIYPDHWFHDQ FE++LKFV KWMLSHIEDGDKELKKPV+FTEFGLS+LNK + P+QR
Sbjct: 291 SVHIYPDHWFHDQGFEEKLKFVVKWMLSHIEDGDKELKKPVLFTEFGLSNLNKDYDPSQR 350

Query: 301 DSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSC 360
           D FY+T++DVIYKSAKR RSGAG+L WQFL+EGME  NDDFGIVPWE+ SI +L+IEQSC
Sbjct: 351 DRFYRTIFDVIYKSAKRKRSGAGTLVWQFLIEGMEGFNDDFGIVPWEQDSIQRLMIEQSC 410

Query: 361 RLARIGGDTQQLKALKYVCAQR 383
           RL+RI G     K    +C+ R
Sbjct: 411 RLSRITGRHLLDKKSIEMCSHR 432

BLAST of CsGy6G010530 vs. TAIR 10
Match: AT4G28320.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 619.4 bits (1596), Expect = 2.0e-177
Identity = 280/382 (73.30%), Postives = 333/382 (87.17%), Query Frame = 0

Query: 1   MLNGKAFYINGWNSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQV 60
           +++ K  Y+NGWNSYWFMDH+V+E+ +  +  ML+A  KMGLTVCRTWAFNDGGYNALQ+
Sbjct: 50  VVDDKPLYVNGWNSYWFMDHAVDEHSRNLVGEMLEAGAKMGLTVCRTWAFNDGGYNALQI 109

Query: 61  SPGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSND 120
           SPGRF+ERVF+ALDHVIAE+R+H +RLLLSLVNNLQAYGGKTQYVKWAWQ+GVGLSSSND
Sbjct: 110 SPGRFDERVFQALDHVIAEARKHDVRLLLSLVNNLQAYGGKTQYVKWAWQEGVGLSSSND 169

Query: 121 SFFYDPSIRIYFKNYLKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEW 180
           SFF+DPSIR YFKNYLK +LTRKNS+TGIEYRNDPTIF WELINEPRC TD SG TLQ+W
Sbjct: 170 SFFFDPSIRNYFKNYLKVLLTRKNSVTGIEYRNDPTIFAWELINEPRCTTDVSGKTLQDW 229

Query: 181 IEEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFA 240
           I+EMT +IKSID KHLLTVGLEGFYGPNSPK  TVNPE+WAS+LG+DF++NS   ++DFA
Sbjct: 230 IDEMTGFIKSIDDKHLLTVGLEGFYGPNSPKGLTVNPEQWASQLGTDFVQNSNSSNIDFA 289

Query: 241 SVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQR 300
           SVHIYPDHWFH+Q FE++LKFV KWM SHIEDG KELKKPV+FTEFGLS+ NK + P+QR
Sbjct: 290 SVHIYPDHWFHNQTFEEKLKFVVKWMQSHIEDGLKELKKPVLFTEFGLSNQNKDYEPSQR 349

Query: 301 DSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSC 360
           D FY+ ++DV+YKSAKR +SGAG+L WQ  +EGME  NDDFGIVP E+ SIY+L+IEQSC
Sbjct: 350 DKFYRIIFDVVYKSAKRKKSGAGTLVWQLFMEGMETFNDDFGIVPHEQDSIYKLMIEQSC 409

Query: 361 RLARIGGDTQQLKALKYVCAQR 383
           RL ++ G  ++ K LK +C+ R
Sbjct: 410 RLGKVTGRLKEQK-LKELCSHR 430

BLAST of CsGy6G010530 vs. TAIR 10
Match: AT5G01930.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 380.6 bits (976), Expect = 1.5e-105
Identity = 173/362 (47.79%), Postives = 250/362 (69.06%), Query Frame = 0

Query: 2   LNGKAFYINGWNSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVS 61
           LNG+ FY+NG+N+YW M  + +   + ++  + Q A  +G+TV RTWAFNDG + ALQ S
Sbjct: 57  LNGQPFYVNGFNTYWMMTLAADNSTRGKVTEVFQQASAVGMTVGRTWAFNDGQWRALQKS 116

Query: 62  PGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDS 121
           P  ++E VFKALD V++E+R++ IRL+LSLVNN  AYGGK QYVKW    G+ L +S+D 
Sbjct: 117 PSVYDEEVFKALDFVLSEARKYKIRLILSLVNNWDAYGGKAQYVKWGNASGLNL-TSDDD 176

Query: 122 FFYDPSIRIYFKNYLKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWI 181
           FF +P++R +++++++TVL R N+ T I Y+NDPTIF WEL+NEPRC +D SGD LQ WI
Sbjct: 177 FFTNPTLRNFYQSHVRTVLNRVNTFTNITYKNDPTIFAWELMNEPRCPSDPSGDKLQSWI 236

Query: 182 EEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFAS 241
           +EM  ++KS+D KHL+ +GLEGFYGP++P ++  NP  +A+++G+DFIRN+++  +DFAS
Sbjct: 237 QEMAVFVKSLDAKHLVEIGLEGFYGPSAPARTRFNPNPYAAQVGTDFIRNNQVLGIDFAS 296

Query: 242 VHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRD 301
           VH+YPD W         L+F S WM +H+ED +  L  PV+FTEFG+S  + GF  + RD
Sbjct: 297 VHVYPDSWISPAVSNSFLEFTSSWMQAHVEDAEMYLGMPVLFTEFGVSAHDPGFNTSFRD 356

Query: 302 SFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCR 361
               TVY +   S ++  +GAGSL WQ   +G E  +D + +      +  ++I  QS R
Sbjct: 357 MMLNTVYKMTLNSTRKGGAGAGSLVWQVFPQGAEFMDDGYAVYLTRAHTASKIISLQSKR 416

Query: 362 LA 364
           LA
Sbjct: 417 LA 417

BLAST of CsGy6G010530 vs. TAIR 10
Match: AT5G66460.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 374.0 bits (959), Expect = 1.4e-103
Identity = 172/364 (47.25%), Postives = 243/364 (66.76%), Query Frame = 0

Query: 2   LNGKAFYINGWNSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQVS 61
           LNG  +Y NG+N+YW M  + +  ++ +I    Q A + GLTV RTWAF+DGGY ALQ S
Sbjct: 41  LNGYPYYANGFNAYWLMYVASDPSQRSKISTAFQDASRHGLTVARTWAFSDGGYRALQYS 100

Query: 62  PGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSNDS 121
           PG +NE +F+ LD  +AE+R+HGI+++LS  NN +++GG+ QYV WA   G  +SS +D 
Sbjct: 101 PGSYNEDMFQGLDFALAEARRHGIKIILSFANNYESFGGRKQYVDWARSRGRPVSSEDD- 160

Query: 122 FFYDPSIRIYFKNYLKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEWI 181
           FF D  ++ ++KN++K VL R N+ T + Y++DPTI  WEL+NEPRC +D SG  +Q WI
Sbjct: 161 FFTDSLVKDFYKNHIKAVLNRFNTFTKVHYKDDPTIMAWELMNEPRCPSDPSGRAIQAWI 220

Query: 182 EEMTAYIKSIDKKHLLTVGLEGFYGPNSPKKSTVNPEEWASRLGSDFIRNSEIQHVDFAS 241
            EM A++KS+D+ HLL  GLEGFYG +SP+  T+NP     + G+DFI N+ I  +DF +
Sbjct: 221 TEMAAHVKSLDRNHLLEAGLEGFYGQSSPQSKTLNP---PGQFGTDFIANNRIPGIDFVT 280

Query: 242 VHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQRD 301
           VH YPD WF D   + ++ F++KW+ +HI+D    L KP++  EFG S    G+TPAQRD
Sbjct: 281 VHSYPDEWFPDSSEQSQMDFLNKWLDAHIQDAQNVLHKPIILAEFGKSMKKPGYTPAQRD 340

Query: 302 SFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQSCR 361
             + TVY  IY SAKR  + AG L WQ LV G++   D +GI+  + SS   +I +QS +
Sbjct: 341 IVFNTVYSKIYGSAKRGGAAAGGLFWQLLVNGIDNFQDGYGIILSQSSSTVNVISQQSRK 400

Query: 362 LARI 366
           L  I
Sbjct: 401 LTLI 400

BLAST of CsGy6G010530 vs. TAIR 10
Match: AT3G10890.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 352.1 bits (902), Expect = 5.8e-97
Identity = 162/368 (44.02%), Postives = 241/368 (65.49%), Query Frame = 0

Query: 1   MLNGKAFYINGWNSYWFMDHSVEEYRKPRIRAMLQAARKMGLTVCRTWAFNDGGYNALQV 60
           +LNGK FY NG+N+YW    + +   + +I  + Q A    LT+ RTW F DGGY ALQ+
Sbjct: 40  ILNGKPFYANGFNAYWLAYEATDSTTRFKITYVFQNATIHDLTIVRTWGFRDGGYRALQI 99

Query: 61  SPGRFNERVFKALDHVIAESRQHGIRLLLSLVNNLQAYGGKTQYVKWAWQDGVGLSSSND 120
           +PG ++E+ F+ LD  IAE+++ GI+++++ VNN   +GG+ QYV WA   G  + SS+D
Sbjct: 100 APGVYDEKTFQGLDFAIAEAKRLGIKMIITFVNNYSDFGGRKQYVDWAKNTGQNV-SSDD 159

Query: 121 SFFYDPSIRIYFKNYLKTVLTRKNSITGIEYRNDPTIFGWELINEPRCMTDASGDTLQEW 180
            F+ +P ++ Y+KN++KT++ R N+ T +EY+++PTI GWEL+NEP+C  D SG TL  W
Sbjct: 160 DFYTNPLVKQYYKNHVKTMVNRVNTFTKVEYKDEPTIMGWELMNEPQCRADPSGKTLTAW 219

Query: 181 IEEMTAYIKSIDKKHLLTVGLEGFYGPNSP-KKSTVNPEEWASRLGSDFIRNSEIQHVDF 240
           + EM  Y+KS+D KHLL+ GLEGFYG +SP +K+++NP   A+ LG+DFI N ++  +DF
Sbjct: 220 MNEMALYVKSVDSKHLLSTGLEGFYGDSSPQRKTSLNPVA-ANVLGTDFIANHKLDAIDF 279

Query: 241 ASVHIYPDHWFHDQDFEDELKFVSKWMLSHIEDGDKELKKPVMFTEFGLSDLNKGFTPAQ 300
           AS+H YPD WF + D +  L  + KW+  H+ED    LKKP++  EFG      G+T AQ
Sbjct: 280 ASIHSYPDLWFPNLDEKSRLNLLRKWLECHLEDAQNILKKPLILGEFGKPTNTPGYTQAQ 339

Query: 301 RDSFYKTVYDVIYKSAKRNRSGAGSLAWQFLVEGMEESNDDFGIVPWERSSIYQLIIEQS 360
           RD+ +   +D IY+SA++    AG+L W  + +GM    D   IV  E S+   +I E+S
Sbjct: 340 RDAVFNATFDTIYESAEKGGPAAGALFWHVISDGMNNFKDPLSIVLSENSTTVNIITEES 399

Query: 361 CRLARIGG 368
            +L  I G
Sbjct: 400 RKLGLIRG 405

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q7Y2233.2e-18577.23Mannan endo-1,4-beta-mannosidase 2 OS=Arabidopsis thaliana OX=3702 GN=MAN2 PE=2 ... [more]
Q9M0H62.8e-17673.30Mannan endo-1,4-beta-mannosidase 5 OS=Arabidopsis thaliana OX=3702 GN=MAN5 PE=2 ... [more]
Q0JJD41.3e-14662.30Mannan endo-1,4-beta-mannosidase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=M... [more]
Q0DCM58.2e-11251.49Mannan endo-1,4-beta-mannosidase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=M... [more]
Q9LZV32.2e-10447.79Mannan endo-1,4-beta-mannosidase 6 OS=Arabidopsis thaliana OX=3702 GN=MAN6 PE=2 ... [more]
Match NameE-valueIdentityDescription
XP_004139946.12.40e-292100.00mannan endo-1,4-beta-mannosidase 2 [Cucumis sativus] >KGN46738.1 hypothetical pr... [more]
XP_008448293.17.68e-28998.96PREDICTED: mannan endo-1,4-beta-mannosidase 2 [Cucumis melo] >TYK04966.1 mannan ... [more]
KAA0062055.18.96e-28898.43mannan endo-1,4-beta-mannosidase 2 [Cucumis melo var. makuwa][more]
XP_038902035.16.75e-28396.87mannan endo-1,4-beta-mannosidase 2-like [Benincasa hispida][more]
XP_023512795.16.85e-27794.99mannan endo-1,4-beta-mannosidase 2-like isoform X2 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A0A0KB351.16e-292100.00Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G127990 PE=3... [more]
A0A5D3C3G03.72e-28998.96Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3BJC23.72e-28998.96Mannan endo-1,4-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103490531 PE=3 SV... [more]
A0A5A7V8364.34e-28898.43Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A6J1FWH91.35e-27694.72Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111448802 P... [more]
Match NameE-valueIdentityDescription
AT2G20680.12.3e-18677.23Glycosyl hydrolase superfamily protein [more]
AT4G28320.12.0e-17773.30Glycosyl hydrolase superfamily protein [more]
AT5G01930.11.5e-10547.79Glycosyl hydrolase superfamily protein [more]
AT5G66460.11.4e-10347.25Glycosyl hydrolase superfamily protein [more]
AT3G10890.15.8e-9744.02Glycosyl hydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 1..366
e-value: 1.1E-128
score: 431.4
NoneNo IPR availablePANTHERPTHR31451:SF61BNAA09G43620D PROTEINcoord: 2..381
IPR001547Glycoside hydrolase, family 5PFAMPF00150Cellulasecoord: 4..327
e-value: 3.4E-16
score: 59.5
IPR045053Mannan endo-1,4-beta-mannosidase-likePANTHERPTHR31451FAMILY NOT NAMEDcoord: 2..381
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 2..350

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy6G010530.2CsGy6G010530.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071704 organic substance metabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016985 mannan endo-1,4-beta-mannosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds