Homology
BLAST of CsGy6G007150 vs. ExPASy Swiss-Prot
Match:
W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)
HSP 1 Score: 532.3 bits (1370), Expect = 6.1e-150
Identity = 269/510 (52.75%), Postives = 362/510 (70.98%), Query Frame = 0
Query: 22 MEDSILQYSAFSILSIAVVVLAI-IKYLLKFNSNKNLPPSPP-SIPIIGHLHLLKLPVHR 81
ME S L Y+ +AVV+L+I +K +NLPPSP ++P+IGHLHL+ +HR
Sbjct: 1 MESSTLLYT-----FLAVVLLSISLKLFPVSRRRRNLPPSPGLALPVIGHLHLIGKLLHR 60
Query: 82 TLQTLSQKYGPVLSLRFGSRSVVVVTSLEAAEECFIKNDIIFANRPNFAVSHCLSYNETT 141
+L LS+KYG V SL+ G+R V+VV+S AAEECF KNDI+FANRP F + + YN TT
Sbjct: 61 SLYDLSKKYGSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTT 120
Query: 142 LGAAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEVSRDGFGKVEFKP 201
+ +PYG+HWR LRR++ +EI + LN IR DEV++ + +Y+ S FGKVE K
Sbjct: 121 MVGSPYGEHWRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKS 180
Query: 202 LVKELTMNVTMRMVAGKRYFGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYID 261
+ EL+ NVTMRMVAGKRYFG++ S EA+ F+A++ E+FE +S P DF+P L++ID
Sbjct: 181 KLSELSFNVTMRMVAGKRYFGQD-VDSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWID 240
Query: 262 IQGFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDII 321
+ + K+ K+ +DAF Q LI E R I+ + M+ H L LQE+QP+YYTD II
Sbjct: 241 FKNYEKKVSKISQEMDAFLQRLIHESR---INKNNV--TMIDHLLSLQESQPEYYTDQII 300
Query: 322 KGLILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLGGDKLIEETDLSKLNY 381
KG+I+ ++L G+DT+AVT+EWAMS LLNHP L+KAR EIE+Q+G ++LIEE DL KL Y
Sbjct: 301 KGIIMVLLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTY 360
Query: 382 LRYIILETLRLYPAGPLLLPHKSSADCRISGYDIPRDTMLLVNAWAIHRDPVLWEDPTSF 441
L II ET RL PA P+L+PH+SS DC++ GYD+P+ T+LLVNAWAIHRDP W++PT F
Sbjct: 361 LHNIISETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLF 420
Query: 442 KPERFENNNGEGDGNNKLMIAFGLGRRACPGTGMANRVMGLMLGTLIQCFDWKSIDGKEV 501
KPER +G + ++ FG+GRR+CPG+G+A RV+GL LG LIQCF+WK I ++
Sbjct: 421 KPER----HGGVELEPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKI 480
Query: 502 DMNEGKGVSMPKAQPLEALCKAREIATKLL 530
DM EG G++MPKAQPLEALCK R I K++
Sbjct: 481 DMAEGSGLTMPKAQPLEALCKPRNILHKVV 495
BLAST of CsGy6G007150 vs. ExPASy Swiss-Prot
Match:
Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)
HSP 1 Score: 525.8 bits (1353), Expect = 5.7e-148
Identity = 265/501 (52.89%), Postives = 354/501 (70.66%), Query Frame = 0
Query: 32 FSILSIAVVVLAIIKYLLKFNSN--KNLPPSPPSIPIIGHLHLLKLPVHRTLQTLSQKYG 91
+S+LS++ ++ IK LLK S KNLPP PP+IPIIG+LH LK P+HRT TLSQ YG
Sbjct: 5 YSLLSLSFII--TIKILLKITSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQTYG 64
Query: 92 PVLSLRFGSRSVVVVTSLEAAEECFIKNDIIFANRPNFAVSHCLSYNETTLGAAPYGDHW 151
+ SL FGSR VVVV+S A ECF KNDII ANRP F + YN TTLG+A YGDHW
Sbjct: 65 DIFSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHW 124
Query: 152 RKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIY-EVSRDGFG--KVEFKPLVKELTM 211
R LRR++T+++L ++RLN+ +RRDE R ++K+ +V +GFG KVE +P + E+T
Sbjct: 125 RNLRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTF 184
Query: 212 NVTMRMVAGKRYFGEEAAKS--SEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQGFM 271
N MRM++GKRY+G++ S EA+ F+ I+ E+ ++ DFLP+L+ +D+
Sbjct: 185 NAMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLE 244
Query: 272 KRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKGLIL 331
KR K++ R +AF +GLI+EHRRG I ++ M+ H LKL E+QP+YY+D +IKGLI
Sbjct: 245 KRCKRIAKRSNAFLEGLIEEHRRGNIHSD--GGTMIDHLLKLSESQPEYYSDHLIKGLIQ 304
Query: 332 TMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLGGDKLIEETDLSKLNYLRYII 391
M+L G+DT+AVTIEW MS LLNHP VLKKA+ E+++Q+G +KL++E DLSKL YL+ II
Sbjct: 305 GMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNII 364
Query: 392 LETLRLYPAGPLLLPHKSSADCRISGYDIPRDTMLLVNAWAIHRDPVLWEDPTSFKPERF 451
ETLRL+P PLLLPH SS DC I +++P+DT++L N W IHRDP W D SFKPERF
Sbjct: 365 SETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERF 424
Query: 452 ENNNGEGDGNNKLMIAFGLGRRACPGTGMANRVMGLMLGTLIQCFDWKSIDGKEVDMNEG 511
E + NK+M AFGLGRRACPG +A R +G +G LIQCF+W+ +++DM EG
Sbjct: 425 EKE----EEVNKVM-AFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEG 484
Query: 512 KGVSMPKAQPLEALCKAREIA 526
KG++MP PL A+CKA IA
Sbjct: 485 KGITMPMKIPLRAMCKALPIA 496
BLAST of CsGy6G007150 vs. ExPASy Swiss-Prot
Match:
Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)
HSP 1 Score: 522.7 bits (1345), Expect = 4.8e-147
Identity = 255/491 (51.93%), Postives = 345/491 (70.26%), Query Frame = 0
Query: 34 ILSIAVVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTLQTLSQKYGPVLS 93
I+S+ +++ + + KNLPP P +PIIG+LH LK P+H T TLSQKYG + S
Sbjct: 9 IISLFFLIITLKVFFNTSRKFKNLPPGPQCLPIIGNLHQLKQPLHHTFHTLSQKYGQIFS 68
Query: 94 LRFGSRSVVVVTSLEAAEECFIKNDIIFANRPNFAVSHCLSYNETTLGAAPYGDHWRKLR 153
L FGSR VVVV+SL A+ECF KNDI+ ANRP+F + YN TT+ +PYGDHWR LR
Sbjct: 69 LWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRNLR 128
Query: 154 RVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEVSRDGFGKVEFKPLVKELTMNVTMRMV 213
R+ ++EIL S RLN+ EIRRDE+ R ++K+ + S +GF +VE +P+ E+T N MRMV
Sbjct: 129 RILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEMTFNTIMRMV 188
Query: 214 AGKRYFGEEAAKS--SEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQGFMKRAKKLI 273
+GKRY+G + S EAR F+ I+ E+ ++ DFL L++ D G KR KK+
Sbjct: 189 SGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGLEKRLKKIS 248
Query: 274 TRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKGLILTMILGGS 333
R DAF QGLIDEHR G+ ++ N M+ H L Q++QP+YYTD IIKGL++ M+L G+
Sbjct: 249 KRTDAFLQGLIDEHRFGKRNS----NTMIDHLLTQQQSQPEYYTDQIIKGLMVVMLLAGT 308
Query: 334 DTTAVTIEWAMSNLLNHPSVLKKARAEIESQLGGDKLIEETDLSKLNYLRYIILETLRLY 393
DT++VTIEWAMSNLLNHP ++KKA+ E+++ +G D+ ++E D+SKL YL+ I+ ETLRL+
Sbjct: 309 DTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYETLRLH 368
Query: 394 PAGPLLLPHKSSADCRISGYDIPRDTMLLVNAWAIHRDPVLWEDPTSFKPERFENNNGEG 453
A PLL+PH SS D + GY+IP++T+L+VNAW IHRDP LW DPT FKPERFE EG
Sbjct: 369 AAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERFEK---EG 428
Query: 454 DGNNKLMIAFGLGRRACPGTGMANRVMGLMLGTLIQCFDWKSIDGKEVDMNEGKGVSMPK 513
+ N +++FGLGRRACPG ++ R GL LG LIQCF+WK I +++DM E KG++ K
Sbjct: 429 EVNK--LLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMVEAKGITAGK 488
Query: 514 AQPLEALCKAR 523
L A+CK R
Sbjct: 489 KTSLNAMCKVR 490
BLAST of CsGy6G007150 vs. ExPASy Swiss-Prot
Match:
P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)
HSP 1 Score: 510.0 bits (1312), Expect = 3.2e-143
Identity = 244/506 (48.22%), Postives = 349/506 (68.97%), Query Frame = 0
Query: 25 SILQYSAFSILSIAVVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTLQTL 84
S+L YS F + + I +++ KNLPP PPS+PIIG+LH LK P+HRT + L
Sbjct: 5 SLLSYSVFYL-----ALFFIFNIVIRARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGL 64
Query: 85 SQKYGPVLSLRFGSRSVVVVTSLEAAEECFIKNDIIFANRPNFAVSHCLSYNETTLGAAP 144
S+KYG V SL FGSR VVVV+S ++CF KND++ ANRP F + YN TTLG+
Sbjct: 65 SEKYGHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTS 124
Query: 145 YGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEVSRDGFGKVEFKPLVKEL 204
YG+HWR LRR++ L++L + R+N+ IRRDE +R + ++ + S F ++E + ++
Sbjct: 125 YGEHWRNLRRITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDM 184
Query: 205 TMNVTMRMVAGKRYFGEEAAKS--SEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQG 264
T N MRM++GKRY+GE+ S EA F+ +V EL + + ++ DF+P+L+++D +
Sbjct: 185 TFNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFEN 244
Query: 265 FMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKGL 324
KR K + + DAF +GLI+EHR + +E N M+ H L LQ++QP+YYTD IIKGL
Sbjct: 245 LEKRLKDISGKTDAFLRGLIEEHR----TKKERANTMIDHLLNLQDSQPEYYTDQIIKGL 304
Query: 325 ILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLGGDKLIEETDLSKLNYLRY 384
L M+L G+D++AVT+EW+MSNLLNHP VLKK + E+++ +G D+L++E+DL KL YL+
Sbjct: 305 ALAMLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKN 364
Query: 385 IILETLRLYPAGPLLLPHKSSADCRISGYDIPRDTMLLVNAWAIHRDPVLWEDPTSFKPE 444
+I ETLRLY PLLLPH +S +C I GY +P+DT++L+NAWAIHRDP LW + T+FKPE
Sbjct: 365 VINETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPE 424
Query: 445 RFENNNGEGDGNNKLMIAFGLGRRACPGTGMANRVMGLMLGTLIQCFDWKSIDGKEVDMN 504
RFE G + +IAFG+GRRACPG G+A R + + L LIQCFDWK I+G ++D+
Sbjct: 425 RFEKK-----GELEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLA 484
Query: 505 EGKGVSMPKAQPLEALCKAREIATKL 529
E G ++ K PL+A+CK+R + K+
Sbjct: 485 ERDGFTLTKLVPLKAMCKSRPVINKV 496
BLAST of CsGy6G007150 vs. ExPASy Swiss-Prot
Match:
Q6WNR0 (Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1)
HSP 1 Score: 504.2 bits (1297), Expect = 1.8e-141
Identity = 243/505 (48.12%), Postives = 348/505 (68.91%), Query Frame = 0
Query: 26 ILQYSAFSILSIAVVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTLQTLS 85
IL Y +S+ ++ + II+ L + KNLPP P S+PIIG+LH LK P++RT + L+
Sbjct: 3 ILSYLCYSLFYLS--IFFIIRLLFQSRKFKNLPPGPTSLPIIGNLHHLKRPLNRTFKALT 62
Query: 86 QKYGPVLSLRFGSRSVVVVTSLEAAEECFIKNDIIFANRPNFAVSHCLSYNETTLGAAPY 145
+KYG V+SL FGSR VVVV+SL +ECF KND++ ANRP F + YN TTLG+ Y
Sbjct: 63 EKYGNVISLWFGSRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSY 122
Query: 146 GDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEVSRDGFGKVEFKPLVKELT 205
G+HWR LRR+++L++L + R+N IRRDE +R +KK+ E S F +VE ++T
Sbjct: 123 GEHWRNLRRITSLDVLSNHRINNFAPIRRDETQRLIKKLAEDSSTKFAEVELTFRFFDMT 182
Query: 206 MNVTMRMVAGKRYFGEEA--AKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQGF 265
N MRM++GKRY+G++ ++ EA F+ +V EL + + ++ DF+P+LK++D +
Sbjct: 183 FNNIMRMISGKRYYGDDCDISEVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLDFENL 242
Query: 266 MKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKGLI 325
KR K++ + D F GL+ E R S +E N M+ H L +QE+QP+YYTD IIKGL
Sbjct: 243 EKRVKRIGEKNDVFLSGLLQEQR----SKKERTNTMIDHLLNMQESQPEYYTDTIIKGLC 302
Query: 326 LTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLGGDKLIEETDLSKLNYLRYI 385
L M+L G+D++AVT+EW MSN+LN+P VLKK R E+++ +G D+L++E+DL KL YLR +
Sbjct: 303 LAMLLAGTDSSAVTLEWTMSNILNYPEVLKKVRDEVDTHVGQDRLVDESDLPKLTYLRNV 362
Query: 386 ILETLRLYPAGPLLLPHKSSADCRISGYDIPRDTMLLVNAWAIHRDPVLWEDPTSFKPER 445
I ETLRLY PLLLPH ++ +C + GY +PRDT++L+NAWAIHRDP W + T+FKPER
Sbjct: 363 IYETLRLYTPAPLLLPHSTADECIMGGYKVPRDTIVLINAWAIHRDPETWSEATTFKPER 422
Query: 446 FENNNGEGDGNNKLMIAFGLGRRACPGTGMANRVMGLMLGTLIQCFDWKSIDGKEVDMNE 505
F+ G + MIAFG+GRRACPG G+A R + + L L+QCFDWK I+ +++DM+E
Sbjct: 423 FDKK-----GELEKMIAFGMGRRACPGEGLALRAISMTLALLVQCFDWKRINDEKIDMSE 482
Query: 506 GKGVSMPKAQPLEALCKAREIATKL 529
G +M K PL+A+CK R + K+
Sbjct: 483 RDGFTMTKLLPLKAMCKTRPVVNKV 496
BLAST of CsGy6G007150 vs. NCBI nr
Match:
KGN46394.2 (hypothetical protein Csa_005702 [Cucumis sativus])
HSP 1 Score: 1056 bits (2732), Expect = 0.0
Identity = 529/529 (100.00%), Postives = 529/529 (100.00%), Query Frame = 0
Query: 1 MCVCVNQPCSLVKEPSSMWEIMEDSILQYSAFSILSIAVVVLAIIKYLLKFNSNKNLPPS 60
MCVCVNQPCSLVKEPSSMWEIMEDSILQYSAFSILSIAVVVLAIIKYLLKFNSNKNLPPS
Sbjct: 1 MCVCVNQPCSLVKEPSSMWEIMEDSILQYSAFSILSIAVVVLAIIKYLLKFNSNKNLPPS 60
Query: 61 PPSIPIIGHLHLLKLPVHRTLQTLSQKYGPVLSLRFGSRSVVVVTSLEAAEECFIKNDII 120
PPSIPIIGHLHLLKLPVHRTLQTLSQKYGPVLSLRFGSRSVVVVTSLEAAEECFIKNDII
Sbjct: 61 PPSIPIIGHLHLLKLPVHRTLQTLSQKYGPVLSLRFGSRSVVVVTSLEAAEECFIKNDII 120
Query: 121 FANRPNFAVSHCLSYNETTLGAAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRA 180
FANRPNFAVSHCLSYNETTLGAAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRA
Sbjct: 121 FANRPNFAVSHCLSYNETTLGAAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRA 180
Query: 181 MKKIYEVSRDGFGKVEFKPLVKELTMNVTMRMVAGKRYFGEEAAKSSEARTFQAIVHELF 240
MKKIYEVSRDGFGKVEFKPLVKELTMNVTMRMVAGKRYFGEEAAKSSEARTFQAIVHELF
Sbjct: 181 MKKIYEVSRDGFGKVEFKPLVKELTMNVTMRMVAGKRYFGEEAAKSSEARTFQAIVHELF 240
Query: 241 EFTLSSYPADFLPILKYIDIQGFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMV 300
EFTLSSYPADFLPILKYIDIQGFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMV
Sbjct: 241 EFTLSSYPADFLPILKYIDIQGFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMV 300
Query: 301 AHFLKLQETQPDYYTDDIIKGLILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIE 360
AHFLKLQETQPDYYTDDIIKGLILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIE
Sbjct: 301 AHFLKLQETQPDYYTDDIIKGLILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIE 360
Query: 361 SQLGGDKLIEETDLSKLNYLRYIILETLRLYPAGPLLLPHKSSADCRISGYDIPRDTMLL 420
SQLGGDKLIEETDLSKLNYLRYIILETLRLYPAGPLLLPHKSSADCRISGYDIPRDTMLL
Sbjct: 361 SQLGGDKLIEETDLSKLNYLRYIILETLRLYPAGPLLLPHKSSADCRISGYDIPRDTMLL 420
Query: 421 VNAWAIHRDPVLWEDPTSFKPERFENNNGEGDGNNKLMIAFGLGRRACPGTGMANRVMGL 480
VNAWAIHRDPVLWEDPTSFKPERFENNNGEGDGNNKLMIAFGLGRRACPGTGMANRVMGL
Sbjct: 421 VNAWAIHRDPVLWEDPTSFKPERFENNNGEGDGNNKLMIAFGLGRRACPGTGMANRVMGL 480
Query: 481 MLGTLIQCFDWKSIDGKEVDMNEGKGVSMPKAQPLEALCKAREIATKLL 529
MLGTLIQCFDWKSIDGKEVDMNEGKGVSMPKAQPLEALCKAREIATKLL
Sbjct: 481 MLGTLIQCFDWKSIDGKEVDMNEGKGVSMPKAQPLEALCKAREIATKLL 529
BLAST of CsGy6G007150 vs. NCBI nr
Match:
NP_001289568.1 (isoflavone 2'-hydroxylase-like [Cucumis sativus] >AIT72031.1 cytochrome P450 [Cucumis sativus])
HSP 1 Score: 1011 bits (2615), Expect = 0.0
Identity = 508/508 (100.00%), Postives = 508/508 (100.00%), Query Frame = 0
Query: 22 MEDSILQYSAFSILSIAVVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTL 81
MEDSILQYSAFSILSIAVVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTL
Sbjct: 1 MEDSILQYSAFSILSIAVVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTL 60
Query: 82 QTLSQKYGPVLSLRFGSRSVVVVTSLEAAEECFIKNDIIFANRPNFAVSHCLSYNETTLG 141
QTLSQKYGPVLSLRFGSRSVVVVTSLEAAEECFIKNDIIFANRPNFAVSHCLSYNETTLG
Sbjct: 61 QTLSQKYGPVLSLRFGSRSVVVVTSLEAAEECFIKNDIIFANRPNFAVSHCLSYNETTLG 120
Query: 142 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEVSRDGFGKVEFKPLV 201
AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEVSRDGFGKVEFKPLV
Sbjct: 121 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEVSRDGFGKVEFKPLV 180
Query: 202 KELTMNVTMRMVAGKRYFGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQ 261
KELTMNVTMRMVAGKRYFGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQ
Sbjct: 181 KELTMNVTMRMVAGKRYFGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQ 240
Query: 262 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKG 321
GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKG
Sbjct: 241 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKG 300
Query: 322 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLGGDKLIEETDLSKLNYLR 381
LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLGGDKLIEETDLSKLNYLR
Sbjct: 301 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLGGDKLIEETDLSKLNYLR 360
Query: 382 YIILETLRLYPAGPLLLPHKSSADCRISGYDIPRDTMLLVNAWAIHRDPVLWEDPTSFKP 441
YIILETLRLYPAGPLLLPHKSSADCRISGYDIPRDTMLLVNAWAIHRDPVLWEDPTSFKP
Sbjct: 361 YIILETLRLYPAGPLLLPHKSSADCRISGYDIPRDTMLLVNAWAIHRDPVLWEDPTSFKP 420
Query: 442 ERFENNNGEGDGNNKLMIAFGLGRRACPGTGMANRVMGLMLGTLIQCFDWKSIDGKEVDM 501
ERFENNNGEGDGNNKLMIAFGLGRRACPGTGMANRVMGLMLGTLIQCFDWKSIDGKEVDM
Sbjct: 421 ERFENNNGEGDGNNKLMIAFGLGRRACPGTGMANRVMGLMLGTLIQCFDWKSIDGKEVDM 480
Query: 502 NEGKGVSMPKAQPLEALCKAREIATKLL 529
NEGKGVSMPKAQPLEALCKAREIATKLL
Sbjct: 481 NEGKGVSMPKAQPLEALCKAREIATKLL 508
BLAST of CsGy6G007150 vs. NCBI nr
Match:
XP_008459942.1 (PREDICTED: isoflavone 3'-hydroxylase-like [Cucumis melo] >KAA0039878.1 isoflavone 3'-hydroxylase-like [Cucumis melo var. makuwa] >TYK24623.1 isoflavone 3'-hydroxylase-like [Cucumis melo var. makuwa])
HSP 1 Score: 977 bits (2525), Expect = 0.0
Identity = 487/508 (95.87%), Postives = 499/508 (98.23%), Query Frame = 0
Query: 22 MEDSILQYSAFSILSIAVVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTL 81
MEDSILQYSAFSILSI VVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTL
Sbjct: 1 MEDSILQYSAFSILSIGVVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTL 60
Query: 82 QTLSQKYGPVLSLRFGSRSVVVVTSLEAAEECFIKNDIIFANRPNFAVSHCLSYNETTLG 141
QTLSQKYGPVLSLRFGSR VVVVTSLEAAEECF KND+IFANRPNFAVSHCLSYNETTLG
Sbjct: 61 QTLSQKYGPVLSLRFGSRLVVVVTSLEAAEECFTKNDVIFANRPNFAVSHCLSYNETTLG 120
Query: 142 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEVSRDGFGKVEFKPLV 201
AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRA+KKI+EVSRDGFGKVEFKPLV
Sbjct: 121 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAVKKIFEVSRDGFGKVEFKPLV 180
Query: 202 KELTMNVTMRMVAGKRYFGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQ 261
KELTMN+TMRMVAGKRY+GEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQ
Sbjct: 181 KELTMNITMRMVAGKRYYGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQ 240
Query: 262 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKG 321
GFMKRAKKLI RVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKG
Sbjct: 241 GFMKRAKKLIARVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKG 300
Query: 322 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLGGDKLIEETDLSKLNYLR 381
LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQL GDKL+EETDLSKL+YLR
Sbjct: 301 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLDGDKLLEETDLSKLSYLR 360
Query: 382 YIILETLRLYPAGPLLLPHKSSADCRISGYDIPRDTMLLVNAWAIHRDPVLWEDPTSFKP 441
I+LETLRLYPAGPLLLPHKSSADC ISGY+IPRDT+LLVNAWAIHRDP LWEDPTSF+P
Sbjct: 361 CIVLETLRLYPAGPLLLPHKSSADCSISGYNIPRDTILLVNAWAIHRDPTLWEDPTSFRP 420
Query: 442 ERFENNNGEGDGNNKLMIAFGLGRRACPGTGMANRVMGLMLGTLIQCFDWKSIDGKEVDM 501
ERFENNNGEGD NNKLMIAFGLGRRACPGTGMA+RVMGLMLGTLIQCFDWKSIDGKEVDM
Sbjct: 421 ERFENNNGEGDANNKLMIAFGLGRRACPGTGMAHRVMGLMLGTLIQCFDWKSIDGKEVDM 480
Query: 502 NEGKGVSMPKAQPLEALCKAREIATKLL 529
NEGKGVSMPKAQPLEALCKAREIATKLL
Sbjct: 481 NEGKGVSMPKAQPLEALCKAREIATKLL 508
BLAST of CsGy6G007150 vs. NCBI nr
Match:
XP_038907094.1 (cytochrome P450 81Q32-like [Benincasa hispida])
HSP 1 Score: 889 bits (2297), Expect = 0.0
Identity = 446/508 (87.80%), Postives = 474/508 (93.31%), Query Frame = 0
Query: 22 MEDSILQYSAFSILSIAVVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTL 81
MEDSIL YSA SILSI +V L IIK+LLK N KNLPP PPSIPIIGHLHLLKLPVHRTL
Sbjct: 1 MEDSILAYSAISILSIGIV-LGIIKHLLKSNCYKNLPPCPPSIPIIGHLHLLKLPVHRTL 60
Query: 82 QTLSQKYGPVLSLRFGSRSVVVVTSLEAAEECFIKNDIIFANRPNFAVSHCLSYNETTLG 141
QTLSQKYGPVLSLRFGSR VVVV+S++AAEECF KNDIIFANRP+FAVS CLSYN TTLG
Sbjct: 61 QTLSQKYGPVLSLRFGSRLVVVVSSMQAAEECFTKNDIIFANRPDFAVSRCLSYNHTTLG 120
Query: 142 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEVSRDGFGKVEFKPLV 201
AAPYGDHWRKLRRVS LEIL S+RLN N IRRDEVRR M+KIYE SRD FGKVEFK +V
Sbjct: 121 AAPYGDHWRKLRRVSALEILSSNRLNTNSGIRRDEVRRVMRKIYEASRDDFGKVEFKSMV 180
Query: 202 KELTMNVTMRMVAGKRYFGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQ 261
KELT+N+TMRMVAGKRY+GEEAAKSSEARTFQAIV+ELFEFTLSSYPADFLPILKYIDIQ
Sbjct: 181 KELTLNITMRMVAGKRYYGEEAAKSSEARTFQAIVNELFEFTLSSYPADFLPILKYIDIQ 240
Query: 262 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKG 321
GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEM+N MVAHFLKLQE+QP+YYTDDIIKG
Sbjct: 241 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMENSMVAHFLKLQESQPEYYTDDIIKG 300
Query: 322 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLGGDKLIEETDLSKLNYLR 381
LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAE+ESQLG D+L+EETDLSKL+YLR
Sbjct: 301 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAELESQLGEDQLLEETDLSKLSYLR 360
Query: 382 YIILETLRLYPAGPLLLPHKSSADCRISGYDIPRDTMLLVNAWAIHRDPVLWEDPTSFKP 441
I+LETLRLYPAGPLLLPHKSSADC I GYD+P DT+LLVNAWAIHRDP LWEDP SFKP
Sbjct: 361 CIVLETLRLYPAGPLLLPHKSSADCTILGYDVPHDTILLVNAWAIHRDPTLWEDPMSFKP 420
Query: 442 ERFENNNGEGDGNNKLMIAFGLGRRACPGTGMANRVMGLMLGTLIQCFDWKSIDGKEVDM 501
ERFENNNGEGDG++KLMIAFGLGRRACPGTGMA+RV+GL LG LIQCFDWKSI+GKEVDM
Sbjct: 421 ERFENNNGEGDGSSKLMIAFGLGRRACPGTGMAHRVLGLSLGLLIQCFDWKSIEGKEVDM 480
Query: 502 NEGKGVSMPKAQPLEALCKAREIATKLL 529
EG GVSMPKAQPLEALCKAREIATKLL
Sbjct: 481 TEGIGVSMPKAQPLEALCKAREIATKLL 507
BLAST of CsGy6G007150 vs. NCBI nr
Match:
KAG7025244.1 (Isoflavone 2'-hydroxylase [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 798 bits (2060), Expect = 2.60e-287
Identity = 397/508 (78.15%), Postives = 450/508 (88.58%), Query Frame = 0
Query: 22 MEDSILQYSAFSILSIAVVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTL 81
M+DS YS +L IA+VV I Y LK S KNLPP+PPSIPIIGHLHLLK PVHR+L
Sbjct: 1 MDDSTFLYS---VLCIALVVA--ISYALKQKSRKNLPPTPPSIPIIGHLHLLKPPVHRSL 60
Query: 82 QTLSQKYGPVLSLRFGSRSVVVVTSLEAAEECFIKNDIIFANRPNFAVSHCLSYNETTLG 141
TLSQKYGPVLSLRFGSR VVVV+S++AAEECF KND++FANRP+FAVS CLSYN TTLG
Sbjct: 61 LTLSQKYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLG 120
Query: 142 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEVSRDGFGKVEFKPLV 201
AAPYGDHWRKLRRVS LEIL ++RLN N IRRDEVRR + K+ + SRD F +VEFK +V
Sbjct: 121 AAPYGDHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQCSRDDFARVEFKSMV 180
Query: 202 KELTMNVTMRMVAGKRYFGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQ 261
KELT+N+TMRMVAGKRY+GEE +SSEARTF+AIV ELFEF L+ YPADFLPILKYIDIQ
Sbjct: 181 KELTLNITMRMVAGKRYYGEETDESSEARTFRAIVDELFEFMLAQYPADFLPILKYIDIQ 240
Query: 262 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKG 321
GFMKRAKKLI RVDAFWQG++DEHR +I N+EM+N MVAHFLKLQE+QP YYTDDIIKG
Sbjct: 241 GFMKRAKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKG 300
Query: 322 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLGGDKLIEETDLSKLNYLR 381
LILTM+LGGSDTTAVTIEWAMSNLLNHP+VLKKARAE+E+QLG D+L+EE+++ KL+YLR
Sbjct: 301 LILTMVLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQLG-DQLLEESEVPKLSYLR 360
Query: 382 YIILETLRLYPAGPLLLPHKSSADCRISGYDIPRDTMLLVNAWAIHRDPVLWEDPTSFKP 441
I+LETLRLYPAGPLLLPHKSSADC +SGYD+PRDT+LLVNAWAIHRDP LWEDPTSF+P
Sbjct: 361 CIVLETLRLYPAGPLLLPHKSSADCTLSGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRP 420
Query: 442 ERFENNNGEGDGNNKLMIAFGLGRRACPGTGMANRVMGLMLGTLIQCFDWKSIDGKEVDM 501
ERFE G+G+ N+KLMIAFGLGRRACPG+GMA+RV+GL LG LIQCFDWKSIDGKEVDM
Sbjct: 421 ERFE--GGDGEANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDM 480
Query: 502 NEGKGVSMPKAQPLEALCKAREIATKLL 529
EGKGVSMPKA+PLEALCK R IA+KLL
Sbjct: 481 TEGKGVSMPKAEPLEALCKPRPIASKLL 500
BLAST of CsGy6G007150 vs. ExPASy TrEMBL
Match:
A0A097IYP1 (Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa6G088160 PE=2 SV=1)
HSP 1 Score: 1011 bits (2615), Expect = 0.0
Identity = 508/508 (100.00%), Postives = 508/508 (100.00%), Query Frame = 0
Query: 22 MEDSILQYSAFSILSIAVVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTL 81
MEDSILQYSAFSILSIAVVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTL
Sbjct: 1 MEDSILQYSAFSILSIAVVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTL 60
Query: 82 QTLSQKYGPVLSLRFGSRSVVVVTSLEAAEECFIKNDIIFANRPNFAVSHCLSYNETTLG 141
QTLSQKYGPVLSLRFGSRSVVVVTSLEAAEECFIKNDIIFANRPNFAVSHCLSYNETTLG
Sbjct: 61 QTLSQKYGPVLSLRFGSRSVVVVTSLEAAEECFIKNDIIFANRPNFAVSHCLSYNETTLG 120
Query: 142 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEVSRDGFGKVEFKPLV 201
AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEVSRDGFGKVEFKPLV
Sbjct: 121 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEVSRDGFGKVEFKPLV 180
Query: 202 KELTMNVTMRMVAGKRYFGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQ 261
KELTMNVTMRMVAGKRYFGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQ
Sbjct: 181 KELTMNVTMRMVAGKRYFGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQ 240
Query: 262 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKG 321
GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKG
Sbjct: 241 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKG 300
Query: 322 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLGGDKLIEETDLSKLNYLR 381
LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLGGDKLIEETDLSKLNYLR
Sbjct: 301 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLGGDKLIEETDLSKLNYLR 360
Query: 382 YIILETLRLYPAGPLLLPHKSSADCRISGYDIPRDTMLLVNAWAIHRDPVLWEDPTSFKP 441
YIILETLRLYPAGPLLLPHKSSADCRISGYDIPRDTMLLVNAWAIHRDPVLWEDPTSFKP
Sbjct: 361 YIILETLRLYPAGPLLLPHKSSADCRISGYDIPRDTMLLVNAWAIHRDPVLWEDPTSFKP 420
Query: 442 ERFENNNGEGDGNNKLMIAFGLGRRACPGTGMANRVMGLMLGTLIQCFDWKSIDGKEVDM 501
ERFENNNGEGDGNNKLMIAFGLGRRACPGTGMANRVMGLMLGTLIQCFDWKSIDGKEVDM
Sbjct: 421 ERFENNNGEGDGNNKLMIAFGLGRRACPGTGMANRVMGLMLGTLIQCFDWKSIDGKEVDM 480
Query: 502 NEGKGVSMPKAQPLEALCKAREIATKLL 529
NEGKGVSMPKAQPLEALCKAREIATKLL
Sbjct: 481 NEGKGVSMPKAQPLEALCKAREIATKLL 508
BLAST of CsGy6G007150 vs. ExPASy TrEMBL
Match:
A0A5D3DLU7 (Isoflavone 3'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G001700 PE=3 SV=1)
HSP 1 Score: 977 bits (2525), Expect = 0.0
Identity = 487/508 (95.87%), Postives = 499/508 (98.23%), Query Frame = 0
Query: 22 MEDSILQYSAFSILSIAVVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTL 81
MEDSILQYSAFSILSI VVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTL
Sbjct: 1 MEDSILQYSAFSILSIGVVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTL 60
Query: 82 QTLSQKYGPVLSLRFGSRSVVVVTSLEAAEECFIKNDIIFANRPNFAVSHCLSYNETTLG 141
QTLSQKYGPVLSLRFGSR VVVVTSLEAAEECF KND+IFANRPNFAVSHCLSYNETTLG
Sbjct: 61 QTLSQKYGPVLSLRFGSRLVVVVTSLEAAEECFTKNDVIFANRPNFAVSHCLSYNETTLG 120
Query: 142 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEVSRDGFGKVEFKPLV 201
AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRA+KKI+EVSRDGFGKVEFKPLV
Sbjct: 121 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAVKKIFEVSRDGFGKVEFKPLV 180
Query: 202 KELTMNVTMRMVAGKRYFGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQ 261
KELTMN+TMRMVAGKRY+GEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQ
Sbjct: 181 KELTMNITMRMVAGKRYYGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQ 240
Query: 262 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKG 321
GFMKRAKKLI RVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKG
Sbjct: 241 GFMKRAKKLIARVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKG 300
Query: 322 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLGGDKLIEETDLSKLNYLR 381
LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQL GDKL+EETDLSKL+YLR
Sbjct: 301 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLDGDKLLEETDLSKLSYLR 360
Query: 382 YIILETLRLYPAGPLLLPHKSSADCRISGYDIPRDTMLLVNAWAIHRDPVLWEDPTSFKP 441
I+LETLRLYPAGPLLLPHKSSADC ISGY+IPRDT+LLVNAWAIHRDP LWEDPTSF+P
Sbjct: 361 CIVLETLRLYPAGPLLLPHKSSADCSISGYNIPRDTILLVNAWAIHRDPTLWEDPTSFRP 420
Query: 442 ERFENNNGEGDGNNKLMIAFGLGRRACPGTGMANRVMGLMLGTLIQCFDWKSIDGKEVDM 501
ERFENNNGEGD NNKLMIAFGLGRRACPGTGMA+RVMGLMLGTLIQCFDWKSIDGKEVDM
Sbjct: 421 ERFENNNGEGDANNKLMIAFGLGRRACPGTGMAHRVMGLMLGTLIQCFDWKSIDGKEVDM 480
Query: 502 NEGKGVSMPKAQPLEALCKAREIATKLL 529
NEGKGVSMPKAQPLEALCKAREIATKLL
Sbjct: 481 NEGKGVSMPKAQPLEALCKAREIATKLL 508
BLAST of CsGy6G007150 vs. ExPASy TrEMBL
Match:
A0A1S3CAW8 (isoflavone 3'-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103498910 PE=3 SV=1)
HSP 1 Score: 977 bits (2525), Expect = 0.0
Identity = 487/508 (95.87%), Postives = 499/508 (98.23%), Query Frame = 0
Query: 22 MEDSILQYSAFSILSIAVVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTL 81
MEDSILQYSAFSILSI VVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTL
Sbjct: 1 MEDSILQYSAFSILSIGVVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTL 60
Query: 82 QTLSQKYGPVLSLRFGSRSVVVVTSLEAAEECFIKNDIIFANRPNFAVSHCLSYNETTLG 141
QTLSQKYGPVLSLRFGSR VVVVTSLEAAEECF KND+IFANRPNFAVSHCLSYNETTLG
Sbjct: 61 QTLSQKYGPVLSLRFGSRLVVVVTSLEAAEECFTKNDVIFANRPNFAVSHCLSYNETTLG 120
Query: 142 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEVSRDGFGKVEFKPLV 201
AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRA+KKI+EVSRDGFGKVEFKPLV
Sbjct: 121 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAVKKIFEVSRDGFGKVEFKPLV 180
Query: 202 KELTMNVTMRMVAGKRYFGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQ 261
KELTMN+TMRMVAGKRY+GEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQ
Sbjct: 181 KELTMNITMRMVAGKRYYGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQ 240
Query: 262 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKG 321
GFMKRAKKLI RVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKG
Sbjct: 241 GFMKRAKKLIARVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKG 300
Query: 322 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLGGDKLIEETDLSKLNYLR 381
LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQL GDKL+EETDLSKL+YLR
Sbjct: 301 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLDGDKLLEETDLSKLSYLR 360
Query: 382 YIILETLRLYPAGPLLLPHKSSADCRISGYDIPRDTMLLVNAWAIHRDPVLWEDPTSFKP 441
I+LETLRLYPAGPLLLPHKSSADC ISGY+IPRDT+LLVNAWAIHRDP LWEDPTSF+P
Sbjct: 361 CIVLETLRLYPAGPLLLPHKSSADCSISGYNIPRDTILLVNAWAIHRDPTLWEDPTSFRP 420
Query: 442 ERFENNNGEGDGNNKLMIAFGLGRRACPGTGMANRVMGLMLGTLIQCFDWKSIDGKEVDM 501
ERFENNNGEGD NNKLMIAFGLGRRACPGTGMA+RVMGLMLGTLIQCFDWKSIDGKEVDM
Sbjct: 421 ERFENNNGEGDANNKLMIAFGLGRRACPGTGMAHRVMGLMLGTLIQCFDWKSIDGKEVDM 480
Query: 502 NEGKGVSMPKAQPLEALCKAREIATKLL 529
NEGKGVSMPKAQPLEALCKAREIATKLL
Sbjct: 481 NEGKGVSMPKAQPLEALCKAREIATKLL 508
BLAST of CsGy6G007150 vs. ExPASy TrEMBL
Match:
A0A6J1H614 (isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111460794 PE=3 SV=1)
HSP 1 Score: 796 bits (2055), Expect = 7.26e-287
Identity = 397/508 (78.15%), Postives = 449/508 (88.39%), Query Frame = 0
Query: 22 MEDSILQYSAFSILSIAVVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTL 81
M+DS YSA L IA+VV I Y LK S+KNLPP+PPSIPIIGHLHLLK PVHR+L
Sbjct: 1 MDDSTFLYSA---LCIALVVA--ISYALKPKSHKNLPPTPPSIPIIGHLHLLKPPVHRSL 60
Query: 82 QTLSQKYGPVLSLRFGSRSVVVVTSLEAAEECFIKNDIIFANRPNFAVSHCLSYNETTLG 141
TLSQKYGPVLSLRFGSR VVVV+S++AAEECF KND++FANRP+FAVS CLSYN TTLG
Sbjct: 61 LTLSQKYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLG 120
Query: 142 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEVSRDGFGKVEFKPLV 201
AAPYGDHWRKLRRVS LEIL ++RLN N IRRDEVRR K+ + SRD F +VEFK +V
Sbjct: 121 AAPYGDHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLANKLSQTSRDDFARVEFKSMV 180
Query: 202 KELTMNVTMRMVAGKRYFGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQ 261
KELT+N+TMRMVAGKRY+GEE +SSEA TF+AIV ELFEF L+ YPADFLPILKYIDIQ
Sbjct: 181 KELTLNITMRMVAGKRYYGEETDESSEAMTFRAIVDELFEFMLAQYPADFLPILKYIDIQ 240
Query: 262 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKG 321
GFMKRAKKLI RVDAFWQG++DEHR +I N+EM+N MVAHFLKLQE+QP YYTDDIIKG
Sbjct: 241 GFMKRAKKLIKRVDAFWQGIVDEHRTNQIHNQEMENSMVAHFLKLQESQPQYYTDDIIKG 300
Query: 322 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLGGDKLIEETDLSKLNYLR 381
LILTM+LGGSDTTAVTIEWAMSNLLNHP+VLKKARAE+E+QLG D+L+EE+++ KL+YLR
Sbjct: 301 LILTMVLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQLG-DQLLEESEVPKLSYLR 360
Query: 382 YIILETLRLYPAGPLLLPHKSSADCRISGYDIPRDTMLLVNAWAIHRDPVLWEDPTSFKP 441
I+LETLRLYPAGPLLLPHKSSADC +SGYD+PRDT+LLVNAWAIHRDP LWEDPTSF+P
Sbjct: 361 CIVLETLRLYPAGPLLLPHKSSADCTLSGYDVPRDTILLVNAWAIHRDPTLWEDPTSFRP 420
Query: 442 ERFENNNGEGDGNNKLMIAFGLGRRACPGTGMANRVMGLMLGTLIQCFDWKSIDGKEVDM 501
ERFE G+G+ N+KLMIAFGLGRRACPG+GMA+RV+GL LG LIQCFDWKSIDGKEVDM
Sbjct: 421 ERFE--GGDGEANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDM 480
Query: 502 NEGKGVSMPKAQPLEALCKAREIATKLL 529
EGKGVSMPKA+PLEALCK R IA+KLL
Sbjct: 481 TEGKGVSMPKAEPLEALCKPRPIASKLL 500
BLAST of CsGy6G007150 vs. ExPASy TrEMBL
Match:
A0A6J1KS03 (isoflavone 2'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111498126 PE=3 SV=1)
HSP 1 Score: 791 bits (2044), Expect = 3.43e-285
Identity = 393/508 (77.36%), Postives = 449/508 (88.39%), Query Frame = 0
Query: 22 MEDSILQYSAFSILSIAVVVLAIIKYLLKFNSNKNLPPSPPSIPIIGHLHLLKLPVHRTL 81
M+DS YS +L IA+VV I Y LK S KNLPP+PPSIPIIGHLHLLK PVHR+L
Sbjct: 1 MDDSTFLYS---VLCIALVVA--ISYALKPKSRKNLPPTPPSIPIIGHLHLLKPPVHRSL 60
Query: 82 QTLSQKYGPVLSLRFGSRSVVVVTSLEAAEECFIKNDIIFANRPNFAVSHCLSYNETTLG 141
TLSQKYGPVLSLRFGSR VVVV+S++AAEECF KND++FANRP+FAVS CLSYN TTLG
Sbjct: 61 LTLSQKYGPVLSLRFGSRLVVVVSSIDAAEECFTKNDVVFANRPDFAVSKCLSYNHTTLG 120
Query: 142 AAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEVSRDGFGKVEFKPLV 201
AAPYGDHWRKLRRVS LEIL ++RLN N IRRDEVRR + K+ + SRD F +VEFK +V
Sbjct: 121 AAPYGDHWRKLRRVSALEILSANRLNTNSAIRRDEVRRLVNKLSQSSRDDFARVEFKSMV 180
Query: 202 KELTMNVTMRMVAGKRYFGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQ 261
KELT+N+TMRMVAGKRY+GEE +SSEARTF+AIV+ELFEF L+ YPADFLPILKYID Q
Sbjct: 181 KELTLNITMRMVAGKRYYGEETNESSEARTFRAIVNELFEFMLAQYPADFLPILKYIDFQ 240
Query: 262 GFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKG 321
GFMKRAKKLI RVDAFWQG++DEHR ++ N+EM+N MVAHFLKLQE+QP YYTDDIIKG
Sbjct: 241 GFMKRAKKLIKRVDAFWQGIVDEHRTNQVHNQEMENSMVAHFLKLQESQPQYYTDDIIKG 300
Query: 322 LILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLGGDKLIEETDLSKLNYLR 381
LILTM+LGGSDTTAVTIEWAMSNLLNHP+VLKKARAE+E+QLG D+L+EE+++ KL+YLR
Sbjct: 301 LILTMVLGGSDTTAVTIEWAMSNLLNHPTVLKKARAELETQLG-DQLLEESEVPKLSYLR 360
Query: 382 YIILETLRLYPAGPLLLPHKSSADCRISGYDIPRDTMLLVNAWAIHRDPVLWEDPTSFKP 441
I+LETLRLYPAGPLLLPHKSSADC +SGY++PRDT+LLVNAWAIHRDP LWEDPTSF+P
Sbjct: 361 CIVLETLRLYPAGPLLLPHKSSADCTLSGYNVPRDTILLVNAWAIHRDPTLWEDPTSFRP 420
Query: 442 ERFENNNGEGDGNNKLMIAFGLGRRACPGTGMANRVMGLMLGTLIQCFDWKSIDGKEVDM 501
ERFE G+ + N+KLMIAFGLGRRACPG+GMA+RV+GL LG LIQCFDWKSIDGKEVDM
Sbjct: 421 ERFE--GGDSEANSKLMIAFGLGRRACPGSGMAHRVLGLTLGLLIQCFDWKSIDGKEVDM 480
Query: 502 NEGKGVSMPKAQPLEALCKAREIATKLL 529
EGKGVSMPKA+PLEALCK R IA+KLL
Sbjct: 481 TEGKGVSMPKAEPLEALCKPRPIASKLL 500
BLAST of CsGy6G007150 vs. TAIR 10
Match:
AT4G37310.1 (cytochrome P450, family 81, subfamily H, polypeptide 1 )
HSP 1 Score: 530.4 bits (1365), Expect = 1.6e-150
Identity = 261/503 (51.89%), Postives = 362/503 (71.97%), Query Frame = 0
Query: 34 ILSIAVVVLAIIKYLLKFNSNKNLPPSPP-SIPIIGHLHLLKLP-VHRTLQTLSQKYGPV 93
IL+ V + + LL NKNLPPSP PIIGHLHLLK P +HRTL LS GPV
Sbjct: 7 ILTTLVAIFIVKIVLLVTKPNKNLPPSPNICFPIIGHLHLLKKPLLHRTLSHLSHSLGPV 66
Query: 94 LSLRFGSRSVVVVTSLEAAEECFI-KNDIIFANRPNFAVSHCLSYNETTLGAAPYGDHWR 153
SLR GSR V+++S AAEECF+ KNDI+ ANRP F + ++Y+ T++ APYGDHWR
Sbjct: 67 FSLRLGSRLAVIISSPTAAEECFLTKNDIVLANRPRFIMGKYVAYDYTSMVTAPYGDHWR 126
Query: 154 KLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEVSRDGFGKVEFKPLVKELTMNVTM 213
LRR++ LE+ ++RLNA+ EIR DEV+ ++K++++S + KVE + L+ LT+NV M
Sbjct: 127 NLRRITALEVFSTNRLNASAEIRHDEVKMLLQKLHDLSVERPAKVELRQLLTGLTLNVIM 186
Query: 214 RMVAGKRYFGE-EAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQGFMKRAKK 273
RM+ GKR+F E + K+ + F+ +V E+ E + + PADFLP L++ D +G +KRAK+
Sbjct: 187 RMMTGKRFFEEDDGGKAGISLEFRELVAEILELSAADNPADFLPALRWFDYKGLVKRAKR 246
Query: 274 LITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKGLILTMILG 333
+ R+D+ QG +DEHR + E KN M+AH L QE +P Y+D IKGLIL M++G
Sbjct: 247 IGERMDSLLQGFLDEHRANK-DRLEFKNTMIAHLLDSQEKEPHNYSDQTIKGLILMMVVG 306
Query: 334 GSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQL---GGDKLIEETDLSKLNYLRYIILE 393
G+DT+A+T+EWAMSNLLNHP +L+ R I++Q+ +L++E DL +NYL+ ++ E
Sbjct: 307 GTDTSALTVEWAMSNLLNHPQILETTRQNIDTQMETSSSRRLLKEEDLVNMNYLKNVVSE 366
Query: 394 TLRLYPAGPLLLPHKSSADCRISGYDIPRDTMLLVNAWAIHRDPVLWEDPTSFKPERFEN 453
TLRLYP PL++PH S+DC I G+++PRDT++LVN WAIHRDP +W+DPTSFKPERFE
Sbjct: 367 TLRLYPVAPLMVPHVPSSDCVIGGFNVPRDTIVLVNLWAIHRDPSVWDDPTSFKPERFEG 426
Query: 454 NNGEGDGNNKLMIAFGLGRRACPGTGMANRVMGLMLGTLIQCFDWKSIDGKEVDMNEGKG 513
++ G N K+M FGLGRRACPG +ANRV+GL+LG++IQCF+W+S G +VDM EG G
Sbjct: 427 SDQFGHYNGKMM-PFGLGRRACPGLSLANRVVGLLLGSMIQCFEWESGSGGQVDMTEGPG 486
Query: 514 VSMPKAQPLEALCKAREIATKLL 530
+S+PKA+PL C+ RE+A++LL
Sbjct: 487 LSLPKAEPLVVTCRTREMASELL 507
BLAST of CsGy6G007150 vs. TAIR 10
Match:
AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )
HSP 1 Score: 513.1 bits (1320), Expect = 2.7e-145
Identity = 255/498 (51.20%), Postives = 352/498 (70.68%), Query Frame = 0
Query: 34 ILSIAVVVLAIIKYLLKFNSNKNLPPSPP-SIPIIGHLHLLKLPVHRTLQTLSQKY--GP 93
I SI VVL++I + K NLPPSP S+P+IGHL LLK P+HRT +LSQ P
Sbjct: 7 IFSILFVVLSLIYLIGKLKRKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNNAP 66
Query: 94 VLSLRFGSRSVVVVTSLEAAEECFIKNDIIFANRPNFAVSHCLSYNETTLGAAPYGDHWR 153
+ SLR G+R V V +S AEECF KND++ ANRPNF ++ ++Y+ TT+ AA YGDHWR
Sbjct: 67 IFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDHWR 126
Query: 154 KLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEVSRDGFGKVEFKPLVKELTMNVTM 213
LRR+ ++EI + RLN+ IR+DE+RR + ++ F KV+ K ++ +LT N +
Sbjct: 127 NLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFNNIL 186
Query: 214 RMVAGKRYFGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQGFMKRAKKL 273
RMVAGKRY+G+ EA+ + ++ ++ + D+LP+L+ + + R KKL
Sbjct: 187 RMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRL--VSDYETRVKKL 246
Query: 274 ITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKGLILTMILGG 333
R+D F QGL+DE R +E N M+ H L LQE+QPDY+TD IIKG +L +IL G
Sbjct: 247 AGRLDEFLQGLVDEKREA----KEKGNTMIDHLLTLQESQPDYFTDRIIKGNMLALILAG 306
Query: 334 SDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLGGDKLIEETDLSKLNYLRYIILETLRL 393
+DT+AVT+EWA+SN+LNHP VL KAR EI+ ++G D+L++E+D+S L YL+ I+ ETLRL
Sbjct: 307 TDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVSETLRL 366
Query: 394 YPAGPLLLPHKSSADCRISGYDIPRDTMLLVNAWAIHRDPVLWEDPTSFKPERFENNNGE 453
YPA P+LLPH +S DC+++GYD+PR T+LL N WAIHRDP LW+DP SFKPERFE
Sbjct: 367 YPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERFEK---- 426
Query: 454 GDGNNKLMIAFGLGRRACPGTGMANRVMGLMLGTLIQCFDWKSIDGKEVDMNEGKGVSMP 513
+G + ++ FGLGRRACPG+G+A+R++ L LG+LIQC +W+ I G+EVDM+EGKGV+MP
Sbjct: 427 -EGEAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKI-GEEVDMSEGKGVTMP 486
Query: 514 KAQPLEALCKAREIATKL 529
KA+PLEA+C+AR K+
Sbjct: 487 KAKPLEAMCRARPSVVKI 492
BLAST of CsGy6G007150 vs. TAIR 10
Match:
AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )
HSP 1 Score: 507.7 bits (1306), Expect = 1.1e-143
Identity = 255/499 (51.10%), Postives = 347/499 (69.54%), Query Frame = 0
Query: 34 ILSIAVVVLAIIKYLLKFNSNKNLPPSPP-SIPIIGHLHLLKLPVHRTLQTLSQKYG--P 93
I + +VL++I + + NLPPSP ++P+IGHL LLK P+HR ++SQ G P
Sbjct: 7 IFTFCFIVLSLIFLIGRIKRKLNLPPSPAWALPVIGHLRLLKPPLHRVFLSVSQSLGDAP 66
Query: 94 VLSLRFGSRSVVVVTSLEAAEECFIKNDIIFANRPNFAVSHCLSYNETTLGAAPYGDHWR 153
++SLR G+R + VV+S AEECF KND+I ANR + +SY +T+ +A Y +HWR
Sbjct: 67 IISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSASYSEHWR 126
Query: 154 KLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEVSRDGFGKVEFKPLVKELTMNVTM 213
LRR+ LEI + RLN+ IRRDE+RR + ++ S GF KVE K + +LT N +
Sbjct: 127 NLRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYGFTKVEMKSMFSDLTFNNII 186
Query: 214 RMVAGKRYFGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDIQGFMKRAKKL 273
RM+AGK Y+G+ EA+ + ++ E + AD++PIL +I R KKL
Sbjct: 187 RMLAGKCYYGDGKEDDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWITYS--ETRIKKL 246
Query: 274 ITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIKGLILTMILGG 333
R+D F QGL+DE R G+ E+ +N MV H L LQETQP+YY D IIKG +L++I GG
Sbjct: 247 AGRLDEFLQGLVDEKREGK---EKKENTMVDHLLCLQETQPEYYMDRIIKGTMLSLIAGG 306
Query: 334 SDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLGGDKLIEETDLSKLNYLRYIILETLRL 393
+DTTAVT+EWA+S+LLN+P VL KAR EI+ +G D+L+EE+D+ L YL+ I+ ETLRL
Sbjct: 307 TDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPNLPYLQNIVSETLRL 366
Query: 394 YPAGPLLLPHKSSADCRISGYDIPRDTMLLVNAWAIHRDPVLWEDPTSFKPERFENNNGE 453
YPA P+LLPH +S DC++ GYD+PR TMLL NAWAIHRDP+LW+DPTSFKPERFE
Sbjct: 367 YPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSFKPERFEK---- 426
Query: 454 GDGNNKLMIAFGLGRRACPGTGMANRVMGLMLGTLIQCFDWKSIDGKEVDMNEGKGVSMP 513
+G K ++ FGLGRRACPG+G+A R++ L LG+LIQCF+W+ I +EVDM EG G++MP
Sbjct: 427 -EGEAKKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGEEEVDMTEGPGLTMP 486
Query: 514 KAQPLEALCKAREIATKLL 530
KA+PLEA+C+AR+ K+L
Sbjct: 487 KARPLEAMCRARDFVGKIL 495
BLAST of CsGy6G007150 vs. TAIR 10
Match:
AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )
HSP 1 Score: 505.8 bits (1301), Expect = 4.3e-143
Identity = 258/509 (50.69%), Postives = 349/509 (68.57%), Query Frame = 0
Query: 24 DSILQYSAFSILSIAVVVLAIIKYLLKFNSNKNLPPSPP-SIPIIGHLHLLKLPVHRTLQ 83
+ IL S S+ ++A+ +L K L NLPPSP S+P+IGHLHLLK P+HRT
Sbjct: 4 NQILILSFLSLFTLAIFLLTRSKRKL------NLPPSPAISLPVIGHLHLLKPPLHRTFL 63
Query: 84 TLSQKYG--PVLSLRFGSRSVVVVTSLEAAEECFIKNDIIFANRPNFAVSHCLSYNETTL 143
+LS+ G PV LR G+R V V++S AEECF KND++ ANRP F +S L YN T L
Sbjct: 64 SLSKSIGNAPVFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYL 123
Query: 144 GAAPYGDHWRKLRRVSTLEILCSSRLNANYEIRRDEVRRAMKKIYEVSRDGFGKVEFKPL 203
+A YGDHWR LRR++ +EI + RLN+ IR+DE+RR + + S GF +VE K L
Sbjct: 124 LSASYGDHWRNLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISHLSRDSLHGFVEVEMKTL 183
Query: 204 VKELTMNVTMRMVAGKRYFGEEAAKSSEARTFQAIVHELFEFTLSSYPADFLPILKYIDI 263
+ L N T+RM+AGKRYFGE+ + +A+ + +V E + P D+L IL++ +
Sbjct: 184 LTNLASNTTIRMLAGKRYFGED---NDDAKLVKNLVSEAVTSAGAGNPIDYLSILRW--V 243
Query: 264 QGFMKRAKKLITRVDAFWQGLIDEHRRGEISNEEMKNCMVAHFLKLQETQPDYYTDDIIK 323
+ KR K L R D F Q L+DE R + +E M+ H L LQ+ QPDYYTD IIK
Sbjct: 244 SSYEKRIKNLGNRFDTFLQKLVDEKR----AEKEKGETMIDHLLALQDIQPDYYTDVIIK 303
Query: 324 GLILTMILGGSDTTAVTIEWAMSNLLNHPSVLKKARAEIESQLGGDKLIEETDLSKLNYL 383
G+ILT+I+ G+DT++VT+EWAMSNLLNHP +LKKAR EI+ ++G D+L++E+D+ L+YL
Sbjct: 304 GIILTLIIAGTDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNLSYL 363
Query: 384 RYIILETLRLYPAGPLLLPHKSSADCRISGYDIPRDTMLLVNAWAIHRDPVLWEDPTSFK 443
+ I+LETLR+YPA PLLLPH SS DC++ GYDIP TM+L NAWA+HRDP +WEDP FK
Sbjct: 364 QSIVLETLRMYPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFK 423
Query: 444 PERFENNNGEGDGNNKLMIAFGLGRRACPGTGMANRVMGLMLGTLIQCFDWKSIDGKEVD 503
PERFE +G + +I+FG+GRRACPG G+A+R++ LG+L+QCF+W+ + VD
Sbjct: 424 PERFEK-----EGEAEKLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFVD 483
Query: 504 MNEGKGVSMPKAQPLEALCKAREIATKLL 530
M E KG ++PKA PL A+CKAR I KL+
Sbjct: 484 MTEDKGATLPKAIPLRAMCKARSIVDKLI 492
BLAST of CsGy6G007150 vs. TAIR 10
Match:
AT3G28740.1 (Cytochrome P450 superfamily protein )
HSP 1 Score: 501.1 bits (1289), Expect = 1.1e-141
Identity = 252/478 (52.72%), Postives = 332/478 (69.46%), Query Frame = 0
Query: 56 NLPPSPP-SIPIIGHLHLLKLPVHRTLQTLSQKY--GPVLSLRFGSRSVVVVTSLEAAEE 115
NLPPSP PIIGHLHLLKLP+HR +LS+ + SL GSR V VV+S AEE
Sbjct: 38 NLPPSPSRPFPIIGHLHLLKLPLHRRFLSLSESLNNAKIFSLSLGSRLVFVVSSHAVAEE 97
Query: 116 CFIKNDIIFANRPNFAVSHCLSYNETTLGAAPYGDHWRKLRRVSTLEILCSSRLNANYEI 175
CF KND++ ANRP F V + YN TT+ A YGD WR LRR+ T+EI S RLN+ I
Sbjct: 98 CFTKNDVVLANRPEFLVGKHIGYNSTTMVGAAYGDSWRNLRRIGTIEIFSSLRLNSFVSI 157
Query: 176 RRDEVRRAMKKIYEVSRDGFGKVEFKPLVKELTMNVTMRMVAGKRYFGEEAAKSSEARTF 235
R+DE+RR + + + S+ GF KVE KPL LT+N +RMVAGKR++G+ +EA+
Sbjct: 158 RQDEIRRLIICLAKNSQHGFVKVEMKPLFMCLTINNIIRMVAGKRFYGDGTENDNEAKHV 217
Query: 236 QAIVHELFEFTLSSYPADFLPILKYIDIQGFMKRAKKLITRVDAFWQGLIDEHRRGEISN 295
+ ++ E+ + AD+ PIL+Y + + K KKL RVD F Q L++E R ++
Sbjct: 218 RQLIAEVVVSGGAGNAADYFPILRY--VTNYEKHVKKLAGRVDEFLQSLVNEKRVEKVKG 277
Query: 296 EEMKNCMVAHFLKLQETQPDYYTDDIIKGLILTMILGGSDTTAVTIEWAMSNLLNHPSVL 355
N M+ H L LQETQPDYYTD IIKG+IL MIL G+DT+A T+EWAMSNLLNHP VL
Sbjct: 278 ----NTMIDHLLSLQETQPDYYTDVIIKGIILVMILAGTDTSAGTLEWAMSNLLNHPEVL 337
Query: 356 KKARAEIESQLGGDKLIEETDLSKLNYLRYIILETLRLYPAGPLLLPHKSSADCRISGYD 415
+KA+ EI+ Q+G D+L+EE D+ KL YL++I+ ETLRLYP P+LLPH +S DC + GYD
Sbjct: 338 RKAKTEIDDQIGVDRLVEEQDIVKLPYLQHIVSETLRLYPVAPMLLPHLASEDCIVDGYD 397
Query: 416 IPRDTMLLVNAWAIHRDPVLWEDPTSFKPERFENNNGEGDGNNKLMIAFGLGRRACPGTG 475
+PR T++LVNAWAIHRDP LWE+P FKPERFE G +K ++ FG+GRR+CPG+G
Sbjct: 398 VPRGTIILVNAWAIHRDPKLWEEPEKFKPERFEKK-----GEDKKLMPFGIGRRSCPGSG 457
Query: 476 MANRVMGLMLGTLIQCFDWKSIDGKEVDMNEG-KGVSMPKAQPLEALCKAREIATKLL 530
+A R++ L LG+L+QCF+W+ ++ K +DM E KG +M KA L+A+CKAR I K+L
Sbjct: 458 LAQRLVTLALGSLVQCFEWERVEEKYLDMRESEKGTTMRKATSLQAMCKARPIVHKVL 504
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
W8JMU7 | 6.1e-150 | 52.75 | Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1 | [more] |
Q6WNQ9 | 5.7e-148 | 52.89 | Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 P... | [more] |
Q6WNQ8 | 4.8e-147 | 51.93 | Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1 | [more] |
P93147 | 3.2e-143 | 48.22 | Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2 | [more] |
Q6WNR0 | 1.8e-141 | 48.12 | Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
KGN46394.2 | 0.0 | 100.00 | hypothetical protein Csa_005702 [Cucumis sativus] | [more] |
NP_001289568.1 | 0.0 | 100.00 | isoflavone 2'-hydroxylase-like [Cucumis sativus] >AIT72031.1 cytochrome P450 [Cu... | [more] |
XP_008459942.1 | 0.0 | 95.87 | PREDICTED: isoflavone 3'-hydroxylase-like [Cucumis melo] >KAA0039878.1 isoflavon... | [more] |
XP_038907094.1 | 0.0 | 87.80 | cytochrome P450 81Q32-like [Benincasa hispida] | [more] |
KAG7025244.1 | 2.60e-287 | 78.15 | Isoflavone 2'-hydroxylase [Cucurbita argyrosperma subsp. argyrosperma] | [more] |
Match Name | E-value | Identity | Description | |
A0A097IYP1 | 0.0 | 100.00 | Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa6G088160 PE=2 SV=1 | [more] |
A0A5D3DLU7 | 0.0 | 95.87 | Isoflavone 3'-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... | [more] |
A0A1S3CAW8 | 0.0 | 95.87 | isoflavone 3'-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103498910 PE=3 SV=1 | [more] |
A0A6J1H614 | 7.26e-287 | 78.15 | isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111460794 PE=... | [more] |
A0A6J1KS03 | 3.43e-285 | 77.36 | isoflavone 2'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111498126 PE=3 ... | [more] |
Match Name | E-value | Identity | Description | |
AT4G37310.1 | 1.6e-150 | 51.89 | cytochrome P450, family 81, subfamily H, polypeptide 1 | [more] |
AT4G37370.1 | 2.7e-145 | 51.20 | cytochrome P450, family 81, subfamily D, polypeptide 8 | [more] |
AT4G37360.1 | 1.1e-143 | 51.10 | cytochrome P450, family 81, subfamily D, polypeptide 2 | [more] |
AT4G37330.1 | 4.3e-143 | 50.69 | cytochrome P450, family 81, subfamily D, polypeptide 4 | [more] |
AT3G28740.1 | 1.1e-141 | 52.72 | Cytochrome P450 superfamily protein | [more] |