CsGy6G002295 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy6G002295
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionDNA-directed RNA polymerase II subunit 4
LocationGy14Chr6: 1681623 .. 1685339 (-)
RNA-Seq ExpressionCsGy6G002295
SyntenyCsGy6G002295
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCAACTAAATACATCCTTAAATTAAAATAGGAAAAGTAATGTTACAATTGAGAGAAAGCAATGTCTTAGGTTCAATCGGGAGAGAAAGCAATGTTTAGGTTCAATCGAGAGAATGTTTTAGGTTGAATCGAGAGAGGAAGCAATAGCAATGTCTTTAACTGAGAGAAAAAAGAGAAAGCAATCTACCAAAAGAAAAAAGCTAACACAATTTTATAAATATTTTTAAAACCACTATGTTTCAAATAAAAGTTTCGATATTTATCCAAATTTCTCCTTTAAAAGCGCCCTCTCTATGATTATTCCGTATCTCTTCCCTTTGTTCAATAACAGTTCATTCGTTTCCTTTCTTCTCCGGCACTTCCGCGGCAGCCAAACCCGCCTCTCGCCGGACACCATCACCACCTAACAACCATCTTTGAGTCTTCTCTTTGCTCCCTCCTTCTCCGCTGCCTCCATTATCGGTCGCCAGTACGCCTCAGCATTCGATTTCTCTATTTTTCCTTCATCACCGCCACGCTGCTACCAGGACCTGCAGAACTCCATCTCGAGGTCTGTCGAAGTTTTTTTTTTATCCTCTTGCTTTTCTTTATTTACAATCTGATCATGAATAGAACGATTAATTTCTTTAAATGCATATATTCCTTTAACTCAAGAGGTTTGCTACTCTAAATCACGGGGTCGAATTCGAATCTTTAAACGTTGACTCTTGTATATCTTGCAAAAATTTTCCTAATTTTGTAATCATATGTTTCACAGTTCTCTTGAACTTATTGTGAAAATTTTGTGCTAAGTCATTCAGTTGGGGCTCAAATGCCATCTAGTTTAGGGTTTATTGCTGCCGACTTTTGAGAGGGTTTTTTAGTTCATGATTATTTGGTTAATGTAAAATTGATTTGGGCTTCATTTAAGTGTGAAACGCATCCATCATTTATCTTTTAAAATTAAAGATTAACCATGAACAAGTTGATCGAGTTTTCGTGTGTAGGCTTGCGAAGTATCTTACTAAGTTACTAGCTCATTAATGGAAAACAATGATTAAAATTATGAAACATAAGAGTGCCTTCATGTATTTAATGAGTTACATCACGAGGACAATTTGTTAAGGTTATAAACTAAATCCAATATTAGCAAATGAGAGAACCTTTTGGGTCATTCTATTTCGAGTTTCTTCTCATCGATCTTAACTTTTATCTAGAATCAGTTTTTAACTTAGGCCTTCTAATTTGGTGTCTTGTAATTTTGATATATTAGTTTAGTTGGGCGAAGTTAACTTTTTTACTTTGTAGATTTAGTTGTAATCTTGCTGTACTCTGTCCCCAAATATTTTAGGTTGACACAGAACTCCAACTTCAAGCATGTCCGGGGAGGAGGAAGAAAATGCTGCTGAGCTTAAAATTGGAGATGGTAGAAATCTATCTATAAATTAAAACTTCCTATCTATATTCAATGAGAATTTAAGTTTCTTGTATATTTATGGTGATGGGTTTCCTTCATATATTTGTAAAATTAGAATTTTATAAGCAACTGATGTATTTAATTTTTTATTTTGCTCAAAAAGTTAGAAACTTAATTTATAATGTTATACCCCATGATATTATATGTATCATTATTGCAATTCTTTGATCCTTCTAGTGGCCAATACCGATAGGGAGACTGTTCTTATGTACAATGCATATAGATATAGTTTAGATTGGAAATGTGGAGTTAATGTTATCTTCAAGCAGTTTTATGGGTAGTGCGGTAATAATCTCTTAGGCCTGAGGGGAGAAAGGCTGAACTTCCTTAGTTCTTTAAGATCGATAGCTATGTGATTATATATTGTAGCCATGTAGTATCTCATAACTTTCTTGGATATTGTTCGGTAAGTCATAGTCATTTGATTGGTGGCTTGATGACACGTCAACAAAAGAAGTTTATTTTGTCTATATCTTCCCATTGTGTTTTACGATATCATGTTGTTTCTCATAATATTTTAAGCTAGCTCATTTACTTCAAAGGAATACTTCCTTCATATGATGTTAAAGGGTCAAATGGCTGAATGCTTTGAGTTCTCTTATATTTTGGATTAATATCATATTGGTTATTAGGTTTTTAAAAAAATTTAGATGAAAATGTGTGATCTATTCCGTATCTAAATTCTTATCTATGATTATTCTGGCAAAAAGATAACCAATTATTGTAAGTTTTTCCATTCTTATGAATCTATGAAAATGCGGACTCAAGAATGACCAAATATTTGGTTTACATAAGTAATTGTGCAAAAGTCAAGCTCTTGCACCCAAATCATTTTGGTGTATATTCTAAGCTTTGAAAGGTAATAACTGGCGTTTACTTTCTTTGGTTACTTGCAGAGTTCTTAAAAGCCAAATGTTTAATGAATTGTGAAGTTTCTCTAATCCTTGAACATAAATATGAGCAACTTCAACAGATGTCCGAGGATCCAACAAATCAAGTTTCCCAGTGAGTTTTATGTATATCCTTTATTGATTCATGAGTTAATTTAGAGAAGCTACTATATAATTTTTTAAAAATAAAATGACGAGAGAATTCTTAATTATGGTCTTATTTTGCAGAGTGTTTGAAAAGTCACTTCAATATGTGAAACGCTTCAGCCGTTATAAGAATCCCGATGCTGTTAGACAAGTTCGAGAGTATCCTTAAAACTATATATGTTGATGTTTTTACTGGGTGAGAAGGCACGTTTCATTTTATTTGATGGCGGGAATGGTCAACTACCAAGCTAAAAACTCTCTTTGGTTAATTATTTTTTTGGGAAATACTTAATAAATTAGAAGTTCACATAACCATACCATTTACCAACGTAATTTTGAATTGAAAGTTTGGGGTATCATTGTTTAGAAATGGATTAAATAATGTGTAATTGTTATCTTTTGAAATATTGAATCTTAACAAACAAACAAACAGAGTTCTAAGCAGGTATCAGTTGGCGGAGTTTGAGGTATGTACTCTAGACACCTTGGTTTAATCATTTTGTGTAATATTATCGTCTCTTTCTTTTTTGAGGAAACGCTTATCTCATTAGTTTCGAAATTTGCAGCTTTGTGTTCTTGGCAACCTTTGCCCTGAAACTGTGGAAGAAGCCATTGCCATGGTACCATCATTGAAGGTATCTTTTGCCACTTTTGTGCTATTGGATTAGCTAAGTATCAATCATTTTTCTGTTTGGTTGAACCGATAGTATATTTATAGTTATCTTGCTAAATCACAGATACAATTTTTTGGAAGTTTACTATTATTGTATTTGTCTCTCAACATTGAACATGCATCAAATTTTAAGATGTTACTTAAAACTTTAAAATTTTGATATGATCTTTTTTCTTTTCCATCTTTTGAAAACAGAAGTTAATCGCTTATGAATGATTTTGTTTTTGCTGGTTTCAGACCAAGGGAAGGGTGCATGACGATGAAGCAATTGAGAAGATGTTGAATGATCTTTCATTAATCAAAAGATTTGAGTAGTTAGTTAAATGTGTAAACAGAATTATACTCTTGCATAATTTAGAGTTATGACTTTATGGGTTAGTTTGTAATTGGGATCCTCAACCGACTTGAGGATCATAAACTCTTTGATATTTACAAGGATTTATTATGATTATGTGTTTGGCTTTGGGGTTTCTATTTTTTAGATTAAATTTTGGTTGGTACTTAGGTGATTAGTTTTAAAAAATTTATAATCTAATTTGGAAAGATGCGTAC

mRNA sequence

TCAACTAAATACATCCTTAAATTAAAATAGGAAAAGTAATGTTACAATTGAGAGAAAGCAATGTCTTAGGTTCAATCGGGAGAGAAAGCAATGTTTAGGTTCAATCGAGAGAATGTTTTAGGTTGAATCGAGAGAGGAAGCAATAGCAATGTCTTTAACTGAGAGAAAAAAGAGAAAGCAATCTACCAAAAGAAAAAAGCTAACACAATTTTATAAATATTTTTAAAACCACTATGTTTCAAATAAAAGTTTCGATATTTATCCAAATTTCTCCTTTAAAAGCGCCCTCTCTATGATTATTCCGTATCTCTTCCCTTTGTTCAATAACAGTTCATTCGTTTCCTTTCTTCTCCGGCACTTCCGCGGCAGCCAAACCCGCCTCTCGCCGGACACCATCACCACCTAACAACCATCTTTGAGTCTTCTCTTTGCTCCCTCCTTCTCCGCTGCCTCCATTATCGGTCGCCAGTACGCCTCAGCATTCGATTTCTCTATTTTTCCTTCATCACCGCCACGCTGCTACCAGGACCTGCAGAACTCCATCTCGAGGTTGACACAGAACTCCAACTTCAAGCATGTCCGGGGAGGAGGAAGAAAATGCTGCTGAGCTTAAAATTGGAGATGAGTTCTTAAAAGCCAAATGTTTAATGAATTGTGAAGTTTCTCTAATCCTTGAACATAAATATGAGCAACTTCAACAGATGTCCGAGGATCCAACAAATCAAGTTTCCCAAGTGTTTGAAAAGTCACTTCAATATGTGAAACGCTTCAGCCGTTATAAGAATCCCGATGCTGTTAGACAAGTTCGAGAAGTTCTAAGCAGGTATCAGTTGGCGGAGTTTGAGCTTTGTGTTCTTGGCAACCTTTGCCCTGAAACTGTGGAAGAAGCCATTGCCATGGTACCATCATTGAAGACCAAGGGAAGGGTGCATGACGATGAAGCAATTGAGAAGATGTTGAATGATCTTTCATTAATCAAAAGATTTGAGTAGTTAGTTAAATGTGTAAACAGAATTATACTCTTGCATAATTTAGAGTTATGACTTTATGGGTTAGTTTGTAATTGGGATCCTCAACCGACTTGAGGATCATAAACTCTTTGATATTTACAAGGATTTATTATGATTATGTGTTTGGCTTTGGGGTTTCTATTTTTTAGATTAAATTTTGGTTGGTACTTAGGTGATTAGTTTTAAAAAATTTATAATCTAATTTGGAAAGATGCGTAC

Coding sequence (CDS)

ATGTCCGGGGAGGAGGAAGAAAATGCTGCTGAGCTTAAAATTGGAGATGAGTTCTTAAAAGCCAAATGTTTAATGAATTGTGAAGTTTCTCTAATCCTTGAACATAAATATGAGCAACTTCAACAGATGTCCGAGGATCCAACAAATCAAGTTTCCCAAGTGTTTGAAAAGTCACTTCAATATGTGAAACGCTTCAGCCGTTATAAGAATCCCGATGCTGTTAGACAAGTTCGAGAAGTTCTAAGCAGGTATCAGTTGGCGGAGTTTGAGCTTTGTGTTCTTGGCAACCTTTGCCCTGAAACTGTGGAAGAAGCCATTGCCATGGTACCATCATTGAAGACCAAGGGAAGGGTGCATGACGATGAAGCAATTGAGAAGATGTTGAATGATCTTTCATTAATCAAAAGATTTGAGTAG

Protein sequence

MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHDDEAIEKMLNDLSLIKRFE*
Homology
[Close]
BLAST of CsGy6G002295 vs. ExPASy Swiss-Prot
Match: O48890 (DNA-directed RNA polymerase II subunit 4 OS=Arabidopsis thaliana OX=3702 GN=NRPB4 PE=1 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 1.2e-68
Identity = 129/138 (93.48%), Postives = 135/138 (97.83%), Query Frame = 0 

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHK+EQLQQ+SEDP NQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKFEQLQQISEDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120
           YVKRFSRYKNPDAVRQVRE+LSR+QL EFELCVLGNLCPETVEEA+AMVPSLKTKGR HD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRHQLTEFELCVLGNLCPETVEEAVAMVPSLKTKGRAHD 120

Query: 121 DEAIEKMLNDLSLIKRFE 139
           DEAIEKMLNDLSL+KRFE
Sbjct: 121 DEAIEKMLNDLSLVKRFE 138

[Close]
BLAST of CsGy6G002295 vs. ExPASy Swiss-Prot
Match: O15514 (DNA-directed RNA polymerase II subunit RPB4 OS=Homo sapiens OX=9606 GN=POLR2D PE=1 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 1.8e-21
Identity = 56/137 (40.88%), Postives = 93/137 (67.88%), Query Frame = 0 

Query: 2   SGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQY 61
           +G+ EE+A++L    EF  A+ L+N EV ++LEH+ +Q +  S +   ++S+VF K+L Y
Sbjct: 10  AGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNE--SAEDEQELSEVFMKTLNY 69

Query: 62  VKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHDD 121
             RFSR+KN + +  VR +L + +L +FEL  L NLCPET EE+ A++PSL  +GR  D+
Sbjct: 70  TARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSL--EGRFEDE 129

Query: 122 EAIEKMLNDLSLIKRFE 139
           E ++++L+D+   + F+
Sbjct: 130 E-LQQILDDIQTKRSFQ 141

[Close]
BLAST of CsGy6G002295 vs. ExPASy Swiss-Prot
Match: Q9D7M8 (DNA-directed RNA polymerase II subunit RPB4 OS=Mus musculus OX=10090 GN=Polr2d PE=1 SV=2)

HSP 1 Score: 103.6 bits (257), Expect = 1.8e-21
Identity = 56/137 (40.88%), Postives = 93/137 (67.88%), Query Frame = 0 

Query: 2   SGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQY 61
           +G+ EE+A++L    EF  A+ L+N EV ++LEH+ +Q +  S +   ++S+VF K+L Y
Sbjct: 10  AGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNE--SAEDEQELSEVFMKTLNY 69

Query: 62  VKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHDD 121
             RFSR+KN + +  VR +L + +L +FEL  L NLCPET EE+ A++PSL  +GR  D+
Sbjct: 70  TARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSL--EGRFEDE 129

Query: 122 EAIEKMLNDLSLIKRFE 139
           E ++++L+D+   + F+
Sbjct: 130 E-LQQILDDIQTKRSFQ 141

[Close]
BLAST of CsGy6G002295 vs. ExPASy Swiss-Prot
Match: Q54S04 (DNA-directed RNA polymerase II subunit rpb4 OS=Dictyostelium discoideum OX=44689 GN=polr2d PE=3 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 1.1e-18
Identity = 55/147 (37.41%), Postives = 90/147 (61.22%), Query Frame = 0 

Query: 4   EEEENAAELKIGDEFLKAKCLMNCEVSLILEHK----------YEQ---LQQMSEDPTNQ 63
           +EEE+   LK   +   AK L+N EV+++LEH+          + Q   L  +  +  N 
Sbjct: 12  QEEEDLTTLKFPRDLKDAKFLLNSEVAILLEHRKGISESEGTEFPQNTLLIHLYYNSIND 71

Query: 64  VSQVFEKSLQYVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVP 123
           + + F K+L Y ++FSRYKN  +++QVR  LS+  L EFE+  L NLCPE  +EA +++P
Sbjct: 72  IIRTFHKTLAYAEKFSRYKNKTSIKQVRTALSKQNLEEFEIASLANLCPEISDEAKSLIP 131

Query: 124 SLKTKGRVHDDEAIEKMLNDLSLIKRF 138
           SLK   ++ DDE ++ +L++LS +++F
Sbjct: 132 SLK---KMEDDE-LQAILDELSNLRKF 154

[Close]
BLAST of CsGy6G002295 vs. ExPASy Swiss-Prot
Match: Q9VEA5 (DNA-directed RNA polymerase II subunit Rpb4 OS=Drosophila melanogaster OX=7227 GN=Rpb4 PE=2 SV=5)

HSP 1 Score: 87.8 bits (216), Expect = 1.0e-16
Identity = 49/126 (38.89%), Postives = 80/126 (63.49%), Query Frame = 0 

Query: 6   EENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQYVKRF 65
           +E+AA+L+   EF  A+ L+  EV ++L+H+  + Q  S D   + S+VF K+  Y   F
Sbjct: 11  DEDAADLQFPKEFENAETLLISEVHMLLDHR--KRQNESADEEQEFSEVFMKTYAYTDSF 70

Query: 66  SRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHDDEAIE 125
            ++KN + +   R +L + +L +FEL  LGNLCPE  EEA A++PSL  +GR  D+E + 
Sbjct: 71  RKFKNKETIMSARSLLMQKKLHKFELAALGNLCPEAPEEAKALIPSL--EGRFEDEE-LR 130

Query: 126 KMLNDL 132
           ++L+D+
Sbjct: 131 QILDDI 131

[Close]
BLAST of CsGy6G002295 vs. NCBI nr
Match: XP_004138390.1 (DNA-directed RNA polymerase II subunit 4 [Cucumis sativus] >XP_008456795.1 PREDICTED: DNA-directed RNA polymerase II subunit 4 [Cucumis melo] >XP_011656444.1 DNA-directed RNA polymerase II subunit 4 [Cucumis sativus] >KAA0051957.1 DNA-directed RNA polymerase II subunit 4 [Cucumis melo var. makuwa] >KGN45845.1 hypothetical protein Csa_005663 [Cucumis sativus] >TYK04594.1 DNA-directed RNA polymerase II subunit 4 [Cucumis melo var. makuwa])

HSP 1 Score: 268 bits (686), Expect = 1.75e-90
Identity = 138/138 (100.00%), Postives = 138/138 (100.00%), Query Frame = 0 

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120
           YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120

Query: 121 DEAIEKMLNDLSLIKRFE 138
           DEAIEKMLNDLSLIKRFE
Sbjct: 121 DEAIEKMLNDLSLIKRFE 138

[Close]
BLAST of CsGy6G002295 vs. NCBI nr
Match: XP_038884885.1 (DNA-directed RNA polymerase II subunit 4 [Benincasa hispida])

HSP 1 Score: 268 bits (685), Expect = 2.49e-90
Identity = 137/138 (99.28%), Postives = 138/138 (100.00%), Query Frame = 0 

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120
           YVKRFSRYKNPDAVRQVRE+LSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120

Query: 121 DEAIEKMLNDLSLIKRFE 138
           DEAIEKMLNDLSLIKRFE
Sbjct: 121 DEAIEKMLNDLSLIKRFE 138

[Close]
BLAST of CsGy6G002295 vs. NCBI nr
Match: XP_022939401.1 (DNA-directed RNA polymerase II subunit 4-like [Cucurbita moschata] >XP_022939402.1 DNA-directed RNA polymerase II subunit 4-like [Cucurbita moschata] >XP_022993195.1 DNA-directed RNA polymerase II subunit 4-like [Cucurbita maxima] >XP_022993196.1 DNA-directed RNA polymerase II subunit 4-like [Cucurbita maxima] >XP_023549816.1 DNA-directed RNA polymerase II subunit 4-like [Cucurbita pepo subsp. pepo] >XP_023549818.1 DNA-directed RNA polymerase II subunit 4-like [Cucurbita pepo subsp. pepo] >KAG6578503.1 DNA-directed RNA polymerase II subunit 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016067.1 DNA-directed RNA polymerase II subunit 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 264 bits (675), Expect = 8.35e-89
Identity = 134/138 (97.10%), Postives = 137/138 (99.28%), Query Frame = 0 

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120
           YVKRFSRYKNPDAVRQVRE+LSRYQLAEFELCVLGNLCPETVEEAI+MVPSLKTKGR HD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHD 120

Query: 121 DEAIEKMLNDLSLIKRFE 138
           DEAIEKMLNDLSLIK+FE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]
BLAST of CsGy6G002295 vs. NCBI nr
Match: XP_022133507.1 (DNA-directed RNA polymerase II subunit 4 [Momordica charantia])

HSP 1 Score: 263 bits (672), Expect = 2.39e-88
Identity = 134/138 (97.10%), Postives = 136/138 (98.55%), Query Frame = 0 

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDP NQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120
           YVKRFSRYKNPDAVRQVRE+LSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGR HD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRAHD 120

Query: 121 DEAIEKMLNDLSLIKRFE 138
           DEAIEKMLNDLSLIK+FE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]
BLAST of CsGy6G002295 vs. NCBI nr
Match: XP_022954766.1 (DNA-directed RNA polymerase II subunit 4 [Cucurbita moschata] >XP_022954773.1 DNA-directed RNA polymerase II subunit 4 [Cucurbita moschata] >XP_022954778.1 DNA-directed RNA polymerase II subunit 4 [Cucurbita moschata] >XP_022990067.1 DNA-directed RNA polymerase II subunit 4 [Cucurbita maxima] >XP_022990068.1 DNA-directed RNA polymerase II subunit 4 [Cucurbita maxima] >XP_022990070.1 DNA-directed RNA polymerase II subunit 4 [Cucurbita maxima] >XP_023536652.1 DNA-directed RNA polymerase II subunit 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 263 bits (671), Expect = 3.40e-88
Identity = 134/138 (97.10%), Postives = 136/138 (98.55%), Query Frame = 0 

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDP NQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120
           YVKRFSRYKNPDAVRQVRE+LSRYQLAEFELCVLGNLCPETVEEAIAMVPSLK KGRVHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKAKGRVHD 120

Query: 121 DEAIEKMLNDLSLIKRFE 138
           DEAIEKMLNDLSLIK+FE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]
BLAST of CsGy6G002295 vs. ExPASy TrEMBL
Match: A0A5D3C019 (DNA-directed RNA polymerase II subunit 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold409G002310 PE=4 SV=1)

HSP 1 Score: 268 bits (686), Expect = 8.49e-91
Identity = 138/138 (100.00%), Postives = 138/138 (100.00%), Query Frame = 0 

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120
           YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120

Query: 121 DEAIEKMLNDLSLIKRFE 138
           DEAIEKMLNDLSLIKRFE
Sbjct: 121 DEAIEKMLNDLSLIKRFE 138

[Close]
BLAST of CsGy6G002295 vs. ExPASy TrEMBL
Match: A0A0A0K7V5 (RPOL4c domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G014650 PE=4 SV=1)

HSP 1 Score: 268 bits (686), Expect = 8.49e-91
Identity = 138/138 (100.00%), Postives = 138/138 (100.00%), Query Frame = 0 

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120
           YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120

Query: 121 DEAIEKMLNDLSLIKRFE 138
           DEAIEKMLNDLSLIKRFE
Sbjct: 121 DEAIEKMLNDLSLIKRFE 138

[Close]
BLAST of CsGy6G002295 vs. ExPASy TrEMBL
Match: A0A1S3C4R9 (DNA-directed RNA polymerase II subunit 4 OS=Cucumis melo OX=3656 GN=LOC103496636 PE=4 SV=1)

HSP 1 Score: 268 bits (686), Expect = 8.49e-91
Identity = 138/138 (100.00%), Postives = 138/138 (100.00%), Query Frame = 0 

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120
           YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120

Query: 121 DEAIEKMLNDLSLIKRFE 138
           DEAIEKMLNDLSLIKRFE
Sbjct: 121 DEAIEKMLNDLSLIKRFE 138

[Close]
BLAST of CsGy6G002295 vs. ExPASy TrEMBL
Match: A0A6J1JVN3 (DNA-directed RNA polymerase II subunit 4-like OS=Cucurbita maxima OX=3661 GN=LOC111489290 PE=4 SV=1)

HSP 1 Score: 264 bits (675), Expect = 4.04e-89
Identity = 134/138 (97.10%), Postives = 137/138 (99.28%), Query Frame = 0 

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120
           YVKRFSRYKNPDAVRQVRE+LSRYQLAEFELCVLGNLCPETVEEAI+MVPSLKTKGR HD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHD 120

Query: 121 DEAIEKMLNDLSLIKRFE 138
           DEAIEKMLNDLSLIK+FE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]
BLAST of CsGy6G002295 vs. ExPASy TrEMBL
Match: A0A6J1FFS7 (DNA-directed RNA polymerase II subunit 4-like OS=Cucurbita moschata OX=3662 GN=LOC111445330 PE=4 SV=1)

HSP 1 Score: 264 bits (675), Expect = 4.04e-89
Identity = 134/138 (97.10%), Postives = 137/138 (99.28%), Query Frame = 0 

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120
           YVKRFSRYKNPDAVRQVRE+LSRYQLAEFELCVLGNLCPETVEEAI+MVPSLKTKGR HD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHD 120

Query: 121 DEAIEKMLNDLSLIKRFE 138
           DEAIEKMLNDLSLIK+FE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]
BLAST of CsGy6G002295 vs. TAIR 10
Match: AT5G09920.1 (RNA polymerase II, Rpb4, core protein )

HSP 1 Score: 260.4 bits (664), Expect = 8.3e-70
Identity = 129/138 (93.48%), Postives = 135/138 (97.83%), Query Frame = 0 

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHK+EQLQQ+SEDP NQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKFEQLQQISEDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120
           YVKRFSRYKNPDAVRQVRE+LSR+QL EFELCVLGNLCPETVEEA+AMVPSLKTKGR HD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRHQLTEFELCVLGNLCPETVEEAVAMVPSLKTKGRAHD 120

Query: 121 DEAIEKMLNDLSLIKRFE 139
           DEAIEKMLNDLSL+KRFE
Sbjct: 121 DEAIEKMLNDLSLVKRFE 138

[Close]
BLAST of CsGy6G002295 vs. TAIR 10
Match: AT4G15950.1 (RNA polymerase II, Rpb4, core protein )

HSP 1 Score: 87.0 bits (214), Expect = 1.3e-17
Identity = 45/118 (38.14%), Postives = 76/118 (64.41%), Query Frame = 0 

Query: 21  AKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREV 80
           A+C+M+CE   IL+    QL  +SEDP+ ++   ++++L YV+    Y NP +VR+V E 
Sbjct: 89  AECMMDCEAFQILDGIKGQLVGLSEDPSIKIPVSYDRALAYVESCVHYTNPQSVRKVLEP 148

Query: 81  LSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHDDEAIEKMLNDLSLIKRFE 139
           L  Y +++ E+CV+ N   E+V+E +A +PSLKTK  V  ++ ++  L +LS +K+ E
Sbjct: 149 LKTYGISDGEMCVIANASSESVDEVLAFIPSLKTKKEV-INQPLQDALEELSKLKKSE 205

The following BLAST results are available for this feature:
BLAST of CsGy6G002295 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O488901.2e-6893.48DNA-directed RNA polymerase II subunit 4 OS=Arabidopsis thaliana OX=3702 GN=NRPB... [more]
O155141.8e-2140.88DNA-directed RNA polymerase II subunit RPB4 OS=Homo sapiens OX=9606 GN=POLR2D PE... [more]
Q9D7M81.8e-2140.88DNA-directed RNA polymerase II subunit RPB4 OS=Mus musculus OX=10090 GN=Polr2d P... [more]
Q54S041.1e-1837.41DNA-directed RNA polymerase II subunit rpb4 OS=Dictyostelium discoideum OX=44689... [more]
Q9VEA51.0e-1638.89DNA-directed RNA polymerase II subunit Rpb4 OS=Drosophila melanogaster OX=7227 G... [more]
BLAST of CsGy6G002295 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004138390.11.75e-90100.00DNA-directed RNA polymerase II subunit 4 [Cucumis sativus] >XP_008456795.1 PREDI... [more]
XP_038884885.12.49e-9099.28DNA-directed RNA polymerase II subunit 4 [Benincasa hispida][more]
XP_022939401.18.35e-8997.10DNA-directed RNA polymerase II subunit 4-like [Cucurbita moschata] >XP_022939402... [more]
XP_022133507.12.39e-8897.10DNA-directed RNA polymerase II subunit 4 [Momordica charantia][more]
XP_022954766.13.40e-8897.10DNA-directed RNA polymerase II subunit 4 [Cucurbita moschata] >XP_022954773.1 DN... [more]
BLAST of CsGy6G002295 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5D3C0198.49e-91100.00DNA-directed RNA polymerase II subunit 4 OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A0A0K7V58.49e-91100.00RPOL4c domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G014650 PE=4... [more]
A0A1S3C4R98.49e-91100.00DNA-directed RNA polymerase II subunit 4 OS=Cucumis melo OX=3656 GN=LOC103496636... [more]
A0A6J1JVN34.04e-8997.10DNA-directed RNA polymerase II subunit 4-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1FFS74.04e-8997.10DNA-directed RNA polymerase II subunit 4-like OS=Cucurbita moschata OX=3662 GN=L... [more]
BLAST of CsGy6G002295 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G09920.18.3e-7093.48RNA polymerase II, Rpb4, core protein [more]
AT4G15950.11.3e-1738.14RNA polymerase II, Rpb4, core protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006590RNA polymerase Rpb4/RPC9, coreSMARTSM00657rpol4neu2coord: 16..138
e-value: 2.6E-58
score: 209.7
IPR005574RNA polymerase subunit RPB4/RPC9PFAMPF03874RNA_pol_Rpb4coord: 23..132
e-value: 1.4E-23
score: 83.5
IPR038324Rpb4/RPC9 superfamilyGENE3D1.20.1250.40coord: 2..138
e-value: 1.4E-51
score: 175.7
NoneNo IPR availablePANTHERPTHR21297DNA-DIRECTED RNA POLYMERASE IIcoord: 1..138
NoneNo IPR availablePANTHERPTHR21297:SF3DNA-DIRECTED RNA POLYMERASE II SUBUNIT 4-LIKEcoord: 1..138
IPR010997HRDC-like superfamilySUPERFAMILY47819HRDC-likecoord: 3..135

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy6G002295.1CsGy6G002295.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0031990 mRNA export from nucleus in response to heat stress
biological_process GO:0000288 nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
biological_process GO:0045948 positive regulation of translational initiation
biological_process GO:0034402 recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex
biological_process GO:0006367 transcription initiation from RNA polymerase II promoter
biological_process GO:0044237 cellular metabolic process
biological_process GO:0006352 DNA-templated transcription, initiation
cellular_component GO:0000932 P-body
cellular_component GO:0005665 RNA polymerase II, core complex
cellular_component GO:0030880 RNA polymerase complex
molecular_function GO:0003899 DNA-directed 5'-3' RNA polymerase activity
molecular_function GO:0000166 nucleotide binding
molecular_function GO:0031369 translation initiation factor binding