CsGy5G029380 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy5G029380
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionDNA-directed RNA polymerase II subunit 4-like
LocationGy14Chr5: 32691488 .. 32695132 (-)
RNA-Seq ExpressionCsGy5G029380
SyntenyCsGy5G029380
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTTCCAAAACTTACACCTACAAATCCCGCCGAATAAAGTGCAAAATATTATGATAACCGTTATTGGCCTTTGTCCGAGTTGTAAACGAAGAAGCTGCCTCTCATTTTGCCGTCACGTCTCGCCGTCGGTAGATCTCTGCGTTTTGTGGTGCGTCCTCTCCTCTCTCGGTTCTTTCTCTATTCTAATTTCAGAGGATGATTGAATCTTCTACACTAGAAGTTGACATTCATTTCCGTTCATCAAGATAACATATCTCTTTTTTCTAATCTAATTAAAAACTGCTGATCGTTATAGTTCATACAAACTTGATTTTGATGCAGTTTTTGGAATTTAAATTATAAGGTTCTGATGTTCACAGCCTCGTAAATTGTATTTCTAAATAATTTAAACCACGTTTGATTAATTGGTAAACTTGATAGAAATCGTGAGATATAAGAGTGTATTCATGTGCTTTATGAGTTACATCATGAGGACAATTTTGTAATGTTATAGACTAAATCGAGTAAAAGTGAATGAAGGAATTTCTTGTGTGATACCTTATTCAAATTCTTCTTCTTTTTTAAGCCTATCAGTTATACGTATATTTAGTTGCTGGCCTTAAATTTTCCCCTTTTTTTTACTGTACTTCTGATGCCTTGCAATTTGGTTGTGAAGTTTGCTGTCTGGGTTGGTATTTTGATTAATTGGTGTAGCTTGAGCTGTTTTTGTAATATCATTTTTATAGATTTAGTTTTGATCTTGGTCTACGCGTTTCCATATAGTGTAGGTTACACAAATCTTTACCTTGAAGCATGTCTGGAGAAGAGGAAGAAAATGCTGCAGAGCTTAAAATTGGGGATGGTACCATTCTTTCTATACATCTCTAATTTTCTATCAATATTCAATTCAATGAGATTTTAAGCCACTAACATATGTTGCAATGTGTTTGCTTTGTGTATTTGAAAATTTTTAATCTATTGAGCAATTCATGTGTTTGATCTTTTAATTTGCTTAAAAGTGAGAAACTTAATTCACTATGTTGTTCCCCATGCCTTAATGTAGCCTTATTGCACATTCTTTGAACCTTCTATTTGCCAATGTGGAGATTGTTTTTATTTTTTTAACAGTGCATTTAGTTTGGACTGGGAATGTGCAGTCAATGTAAACATTTTCTTAAAGAAATTTTGTGGTTAGAGTGATAGCTTAAAGATATATTTTTTTTCTAAGAAATCTAGATTTAATTGAGAAAAGCGAAGGTGTACTAGTGCATGCAAATAAGCCCACAAAAGGAGTACTCCTCGATTGTAATGCAAAGAAGTTGCAATCTCTTTCATTAAATCACATACCTCGTGGATGGTTGTTTTGCTTGGACGAGAGAGGAGAAATATAGTAAACTGCCTTGTTTCTTTAAGGCCATTAGTTGATGCGATTACATCTTGTACCCATCTGTTCAGGATCGTAGAATCTCTTAAATATTTCAAATATTGTTAGGTAAAGAAACAGTCATTAGATAGGTAGTTGGATGTCCATGTTTATGAAAGGAAGTTTCGTTTGCATGCACCTTCCATTTGTTGTTTATGATATCGTGCTATTATTTTTCGTAATGCATGTTCTTAACATTTTTAGCGGGTACATTTACTATGTTGAAGCATCACTTAATAATGTGAAATTTGGCCAATTGCATCTAAAAAGCCCTAGTCCTTGTGACGTGATTAAGGCACCTCACTTTATACTTGTTTAAGGTTGAAATAGCAAAATGTTAGTGATCTCCACAAAATCTTAATTCTAATCTATAATCATTTTGTCAGTAAGACACTAAAACTATCGCTAGGTTTTCCAGGATAATGCATAAGGCCTGAGACTAGCCAAATCTTTGTGATTTAAATGGATAAGTAATTATTCAAAATTTAAGCATTTGCCATCAAAATCTTTCTAGGGGGTACTCTAGCAGCCTTATTTTCTTGTATTTATCTTGCTCTTTGTGCTAAGATCCATGAAGAGAAATATAAGGCATCATAATTAACACTTTATTTGTCTACTTGCAGAGTTCTTAAAAGCTAAGTGTTTAATGAATTGTGAAGTTTCTTTGATCCTGGAACACAAGTATGAGCAGCTTCAACAAAGGGCTGAGGATCCAACAAATCAAGTTTCTCAGTGAGATTTATGCTTTTTCTTTATTGATTCATAAGTTAAGTTAGAGTTTCTCTTCTTTGTTGGAATAGCTAAAGTGTATAGTTTTATTACGGTATTCATTCTCACTTGCCATACAATAACAATGGATTTTTAATTTGGTCTTCTTTTGCAGAGTGTTTGAGAAGTCACTGCAGTATGTGAAACGTTTCAGTCGCTATAAGAACCCCGATGCTGTTAGACAAGTCCGAGAGTATCCTTTCTTAAATCTTCGATTCACCCAGAAGCTTAAGCTAGTGGGTGAAAGCAAATTTAAACTTATGCCTAACATTTTCTCCTCACTTGTGAGCTCAAAATAGGAAGAAAAATCCAACAAGTGGAAATGAATATTAAATCGAAAAGAAACGTCGAACTTAGACTACACTGCTCATGTACTATCTTAAATCACCGATTCACCCAAAAGCTTAAACTAGTGGGTGAAATCAAATTTAATATTGTATCTAACATAAACTATATAATGTTTTTTATTTTATGGGATGGTGGGAAGGATGAGTTGTGCATATCTACTCTATGCCATAACTAACCCCATTTGGAACTAGTAATTTACATTTGTCATATTTCTTGCTAGACCAATAACTCTCTGATTAGCTGTTACATATATATGTATATATGTATACACATCCATACTATTTAGTATTTTGTAGGTTTCCTCCATATCCATTTGAAAAAAACTATACTACTTCGGTATTTAATTCATGGAGGGAAATACAATTTAAGTTAAAAGAACAGAAAAGTTTGCCTCATTGTTTAAAACGGCATAAATATGGTTAAATCTTAACTATCATATAGAATTCTAAGCAGATATCAACTAGCTGAGTTCGAGGTATGTGTATGAGATCACTTATTAATTAACTTATTGACTATTGTTCTATGGAAAGGTTTATCACATTGTTCTTGAAATTTGCAGTTATGTGTTCTTGGCAACCTTTGCCCTGAAACTGTGGAGGAAGCTACTTCCATAGTTCCATCTTTGAAGGTATTTTTTTTTGCCAACTTTTTATAACTATGCCTGTATTTAATTATTTATTGTTATTTTGATGTGCCATAAGTAGGTACTGACTTTTAAAATGTTTGTTTGATGTGTTTATCTTTTAACATTGAATATGCATCAAGTTTTTGAATTAGGTGAAAAATCCTTTGAGGTATTCTTGACACCAAAATAGTCGAAAACTAATGATAAAAGTATTTCTTGAGCTGATTTGAAAGTTTGAAGGTATTTTTGAAACTTTTGAAACTTCAAAAACATTTTTGATACAAAATGTAAAATTTTGAGACATTTTCTATATTTTAGCTTTTAAGTTTTAAGTTACTGCTAAAATTTTAAAGTGAAAACAAAATGTTCATATATAGATGTATCAAAGTAGACTTTGCTTATAAATACTTTTCTTGGTTTCAGACTAAGGGAAGAGTGCACGAGGACGAAGCAATAGAGAAGATGTTGAATGATCTTTCATTAATAAAAAAGTTCGAGTAG

mRNA sequence

ATTTTCCAAAACTTACACCTACAAATCCCGCCGAATAAAGTGCAAAATATTATGATAACCGTTATTGGCCTTTGTCCGAGTTGTAAACGAAGAAGCTGCCTCTCATTTTGCCGTCACGTCTCGCCGTCGGTAGATCTCTGCGTTTTGTGGTTACACAAATCTTTACCTTGAAGCATGTCTGGAGAAGAGGAAGAAAATGCTGCAGAGCTTAAAATTGGGGATGAGTTCTTAAAAGCTAAGTGTTTAATGAATTGTGAAGTTTCTTTGATCCTGGAACACAAGTATGAGCAGCTTCAACAAAGGGCTGAGGATCCAACAAATCAAGTTTCTCAAGTGTTTGAGAAGTCACTGCAGTATGTGAAACGTTTCAGTCGCTATAAGAACCCCGATGCTGTTAGACAAGTCCGAGAAATTCTAAGCAGATATCAACTAGCTGAGTTCGAGTTATGTGTTCTTGGCAACCTTTGCCCTGAAACTGTGGAGGAAGCTACTTCCATAGTTCCATCTTTGAAGACTAAGGGAAGAGTGCACGAGGACGAAGCAATAGAGAAGATGTTGAATGATCTTTCATTAATAAAAAAGTTCGAGTAG

Coding sequence (CDS)

ATGTCTGGAGAAGAGGAAGAAAATGCTGCAGAGCTTAAAATTGGGGATGAGTTCTTAAAAGCTAAGTGTTTAATGAATTGTGAAGTTTCTTTGATCCTGGAACACAAGTATGAGCAGCTTCAACAAAGGGCTGAGGATCCAACAAATCAAGTTTCTCAAGTGTTTGAGAAGTCACTGCAGTATGTGAAACGTTTCAGTCGCTATAAGAACCCCGATGCTGTTAGACAAGTCCGAGAAATTCTAAGCAGATATCAACTAGCTGAGTTCGAGTTATGTGTTCTTGGCAACCTTTGCCCTGAAACTGTGGAGGAAGCTACTTCCATAGTTCCATCTTTGAAGACTAAGGGAAGAGTGCACGAGGACGAAGCAATAGAGAAGATGTTGAATGATCTTTCATTAATAAAAAAGTTCGAGTAG

Protein sequence

MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHEDEAIEKMLNDLSLIKKFE*
Homology
[Close]
BLAST of CsGy5G029380 vs. ExPASy Swiss-Prot
Match: O48890 (DNA-directed RNA polymerase II subunit 4 OS=Arabidopsis thaliana OX=3702 GN=NRPB4 PE=1 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 5.5e-66
Identity = 125/138 (90.58%), Postives = 133/138 (96.38%), Query Frame = 0 

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHK+EQLQQ +EDP NQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKFEQLQQISEDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHE 120
           YVKRFSRYKNPDAVRQVREILSR+QL EFELCVLGNLCPETVEEA ++VPSLKTKGR H+
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRHQLTEFELCVLGNLCPETVEEAVAMVPSLKTKGRAHD 120

Query: 121 DEAIEKMLNDLSLIKKFE 139
           DEAIEKMLNDLSL+K+FE
Sbjct: 121 DEAIEKMLNDLSLVKRFE 138

[Close]
BLAST of CsGy5G029380 vs. ExPASy Swiss-Prot
Match: O15514 (DNA-directed RNA polymerase II subunit RPB4 OS=Homo sapiens OX=9606 GN=POLR2D PE=1 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 6.3e-22
Identity = 58/137 (42.34%), Postives = 94/137 (68.61%), Query Frame = 0 

Query: 2   SGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQY 61
           +G+ EE+A++L    EF  A+ L+N EV ++LEH+ +Q  + AED   ++S+VF K+L Y
Sbjct: 10  AGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQ-NESAED-EQELSEVFMKTLNY 69

Query: 62  VKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHED 121
             RFSR+KN + +  VR +L + +L +FEL  L NLCPET EE+ +++PSL  +GR  ED
Sbjct: 70  TARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSL--EGR-FED 129

Query: 122 EAIEKMLNDLSLIKKFE 139
           E ++++L+D+   + F+
Sbjct: 130 EELQQILDDIQTKRSFQ 141

[Close]
BLAST of CsGy5G029380 vs. ExPASy Swiss-Prot
Match: Q9D7M8 (DNA-directed RNA polymerase II subunit RPB4 OS=Mus musculus OX=10090 GN=Polr2d PE=1 SV=2)

HSP 1 Score: 105.1 bits (261), Expect = 6.3e-22
Identity = 58/137 (42.34%), Postives = 94/137 (68.61%), Query Frame = 0 

Query: 2   SGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQY 61
           +G+ EE+A++L    EF  A+ L+N EV ++LEH+ +Q  + AED   ++S+VF K+L Y
Sbjct: 10  AGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQ-NESAED-EQELSEVFMKTLNY 69

Query: 62  VKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHED 121
             RFSR+KN + +  VR +L + +L +FEL  L NLCPET EE+ +++PSL  +GR  ED
Sbjct: 70  TARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSL--EGR-FED 129

Query: 122 EAIEKMLNDLSLIKKFE 139
           E ++++L+D+   + F+
Sbjct: 130 EELQQILDDIQTKRSFQ 141

[Close]
BLAST of CsGy5G029380 vs. ExPASy Swiss-Prot
Match: Q54S04 (DNA-directed RNA polymerase II subunit rpb4 OS=Dictyostelium discoideum OX=44689 GN=polr2d PE=3 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 2.2e-19
Identity = 54/147 (36.73%), Postives = 85/147 (57.82%), Query Frame = 0 

Query: 4   EEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAED-------------PTNQ 63
           +EEE+   LK   +   AK L+N EV+++LEH+    +    +               N 
Sbjct: 12  QEEEDLTTLKFPRDLKDAKFLLNSEVAILLEHRKGISESEGTEFPQNTLLIHLYYNSIND 71

Query: 64  VSQVFEKSLQYVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVP 123
           + + F K+L Y ++FSRYKN  +++QVR  LS+  L EFE+  L NLCPE  +EA S++P
Sbjct: 72  IIRTFHKTLAYAEKFSRYKNKTSIKQVRTALSKQNLEEFEIASLANLCPEISDEAKSLIP 131

Query: 124 SLKTKGRVHEDEAIEKMLNDLSLIKKF 138
           SLK      ED+ ++ +L++LS ++KF
Sbjct: 132 SLKKM----EDDELQAILDELSNLRKF 154

[Close]
BLAST of CsGy5G029380 vs. ExPASy Swiss-Prot
Match: Q9VEA5 (DNA-directed RNA polymerase II subunit Rpb4 OS=Drosophila melanogaster OX=7227 GN=Rpb4 PE=2 SV=5)

HSP 1 Score: 89.0 bits (219), Expect = 4.7e-17
Identity = 48/126 (38.10%), Postives = 80/126 (63.49%), Query Frame = 0 

Query: 6   EENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQYVKRF 65
           +E+AA+L+   EF  A+ L+  EV ++L+H+  + Q  + D   + S+VF K+  Y   F
Sbjct: 11  DEDAADLQFPKEFENAETLLISEVHMLLDHR--KRQNESADEEQEFSEVFMKTYAYTDSF 70

Query: 66  SRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHEDEAIE 125
            ++KN + +   R +L + +L +FEL  LGNLCPE  EEA +++PSL  +GR  EDE + 
Sbjct: 71  RKFKNKETIMSARSLLMQKKLHKFELAALGNLCPEAPEEAKALIPSL--EGR-FEDEELR 130

Query: 126 KMLNDL 132
           ++L+D+
Sbjct: 131 QILDDI 131

[Close]
BLAST of CsGy5G029380 vs. NCBI nr
Match: XP_004141497.1 (DNA-directed RNA polymerase II subunit 4 [Cucumis sativus] >XP_031742047.1 DNA-directed RNA polymerase II subunit 4 [Cucumis sativus] >KAE8648963.1 hypothetical protein Csa_008563 [Cucumis sativus])

HSP 1 Score: 268 bits (684), Expect = 3.54e-90
Identity = 138/138 (100.00%), Postives = 138/138 (100.00%), Query Frame = 0 

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHE 120
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHE
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHE 120

Query: 121 DEAIEKMLNDLSLIKKFE 138
           DEAIEKMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]
BLAST of CsGy5G029380 vs. NCBI nr
Match: XP_008459458.1 (PREDICTED: DNA-directed RNA polymerase II subunit 4-like [Cucumis melo] >XP_008459459.1 PREDICTED: DNA-directed RNA polymerase II subunit 4-like [Cucumis melo] >KAA0039380.1 DNA-directed RNA polymerase II subunit 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 266 bits (680), Expect = 1.44e-89
Identity = 136/138 (98.55%), Postives = 138/138 (100.00%), Query Frame = 0 

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQ+SQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQISQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHE 120
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVH+
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHD 120

Query: 121 DEAIEKMLNDLSLIKKFE 138
           DEAIEKMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]
BLAST of CsGy5G029380 vs. NCBI nr
Match: XP_038890773.1 (DNA-directed RNA polymerase II subunit 4-like [Benincasa hispida] >XP_038890774.1 DNA-directed RNA polymerase II subunit 4-like [Benincasa hispida])

HSP 1 Score: 264 bits (674), Expect = 1.19e-88
Identity = 135/138 (97.83%), Postives = 137/138 (99.28%), Query Frame = 0 

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHE 120
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEA S+VPSLKTKGRVH+
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISVVPSLKTKGRVHD 120

Query: 121 DEAIEKMLNDLSLIKKFE 138
           DEAIEKMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]
BLAST of CsGy5G029380 vs. NCBI nr
Match: XP_022939401.1 (DNA-directed RNA polymerase II subunit 4-like [Cucurbita moschata] >XP_022939402.1 DNA-directed RNA polymerase II subunit 4-like [Cucurbita moschata] >XP_022993195.1 DNA-directed RNA polymerase II subunit 4-like [Cucurbita maxima] >XP_022993196.1 DNA-directed RNA polymerase II subunit 4-like [Cucurbita maxima] >XP_023549816.1 DNA-directed RNA polymerase II subunit 4-like [Cucurbita pepo subsp. pepo] >XP_023549818.1 DNA-directed RNA polymerase II subunit 4-like [Cucurbita pepo subsp. pepo] >KAG6578503.1 DNA-directed RNA polymerase II subunit 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016067.1 DNA-directed RNA polymerase II subunit 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 258 bits (659), Expect = 2.30e-86
Identity = 132/138 (95.65%), Postives = 135/138 (97.83%), Query Frame = 0 

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQ +EDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHE 120
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEA S+VPSLKTKGR H+
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHD 120

Query: 121 DEAIEKMLNDLSLIKKFE 138
           DEAIEKMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]
BLAST of CsGy5G029380 vs. NCBI nr
Match: XP_038884885.1 (DNA-directed RNA polymerase II subunit 4 [Benincasa hispida])

HSP 1 Score: 257 bits (657), Expect = 4.64e-86
Identity = 131/138 (94.93%), Postives = 136/138 (98.55%), Query Frame = 0 

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQ +EDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHE 120
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEA ++VPSLKTKGRVH+
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120

Query: 121 DEAIEKMLNDLSLIKKFE 138
           DEAIEKMLNDLSLIK+FE
Sbjct: 121 DEAIEKMLNDLSLIKRFE 138

[Close]
BLAST of CsGy5G029380 vs. ExPASy TrEMBL
Match: A0A1S3CA78 (DNA-directed RNA polymerase II subunit 4-like OS=Cucumis melo OX=3656 GN=LOC103498588 PE=4 SV=1)

HSP 1 Score: 266 bits (680), Expect = 6.98e-90
Identity = 136/138 (98.55%), Postives = 138/138 (100.00%), Query Frame = 0 

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQ+SQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQISQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHE 120
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVH+
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHD 120

Query: 121 DEAIEKMLNDLSLIKKFE 138
           DEAIEKMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]
BLAST of CsGy5G029380 vs. ExPASy TrEMBL
Match: A0A5A7T811 (DNA-directed RNA polymerase II subunit 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold64G001820 PE=4 SV=1)

HSP 1 Score: 266 bits (680), Expect = 6.98e-90
Identity = 136/138 (98.55%), Postives = 138/138 (100.00%), Query Frame = 0 

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQ+SQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQISQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHE 120
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVH+
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHD 120

Query: 121 DEAIEKMLNDLSLIKKFE 138
           DEAIEKMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]
BLAST of CsGy5G029380 vs. ExPASy TrEMBL
Match: A0A6J1JVN3 (DNA-directed RNA polymerase II subunit 4-like OS=Cucurbita maxima OX=3661 GN=LOC111489290 PE=4 SV=1)

HSP 1 Score: 258 bits (659), Expect = 1.11e-86
Identity = 132/138 (95.65%), Postives = 135/138 (97.83%), Query Frame = 0 

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQ +EDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHE 120
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEA S+VPSLKTKGR H+
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHD 120

Query: 121 DEAIEKMLNDLSLIKKFE 138
           DEAIEKMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]
BLAST of CsGy5G029380 vs. ExPASy TrEMBL
Match: A0A6J1FFS7 (DNA-directed RNA polymerase II subunit 4-like OS=Cucurbita moschata OX=3662 GN=LOC111445330 PE=4 SV=1)

HSP 1 Score: 258 bits (659), Expect = 1.11e-86
Identity = 132/138 (95.65%), Postives = 135/138 (97.83%), Query Frame = 0 

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQ +EDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHE 120
           YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEA S+VPSLKTKGR H+
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHD 120

Query: 121 DEAIEKMLNDLSLIKKFE 138
           DEAIEKMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]
BLAST of CsGy5G029380 vs. ExPASy TrEMBL
Match: A0A5D3C019 (DNA-directed RNA polymerase II subunit 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold409G002310 PE=4 SV=1)

HSP 1 Score: 257 bits (656), Expect = 3.19e-86
Identity = 130/138 (94.20%), Postives = 136/138 (98.55%), Query Frame = 0 

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQ +EDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHE 120
           YVKRFSRYKNPDAVRQVRE+LSRYQLAEFELCVLGNLCPETVEEA ++VPSLKTKGRVH+
Sbjct: 61  YVKRFSRYKNPDAVRQVREVLSRYQLAEFELCVLGNLCPETVEEAIAMVPSLKTKGRVHD 120

Query: 121 DEAIEKMLNDLSLIKKFE 138
           DEAIEKMLNDLSLIK+FE
Sbjct: 121 DEAIEKMLNDLSLIKRFE 138

[Close]
BLAST of CsGy5G029380 vs. TAIR 10
Match: AT5G09920.1 (RNA polymerase II, Rpb4, core protein )

HSP 1 Score: 251.5 bits (641), Expect = 3.9e-67
Identity = 125/138 (90.58%), Postives = 133/138 (96.38%), Query Frame = 0 

Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHK+EQLQQ +EDP NQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKFEQLQQISEDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHE 120
           YVKRFSRYKNPDAVRQVREILSR+QL EFELCVLGNLCPETVEEA ++VPSLKTKGR H+
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRHQLTEFELCVLGNLCPETVEEAVAMVPSLKTKGRAHD 120

Query: 121 DEAIEKMLNDLSLIKKFE 139
           DEAIEKMLNDLSL+K+FE
Sbjct: 121 DEAIEKMLNDLSLVKRFE 138

[Close]
BLAST of CsGy5G029380 vs. TAIR 10
Match: AT4G15950.1 (RNA polymerase II, Rpb4, core protein )

HSP 1 Score: 82.8 bits (203), Expect = 2.4e-16
Identity = 44/118 (37.29%), Postives = 74/118 (62.71%), Query Frame = 0 

Query: 21  AKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQYVKRFSRYKNPDAVRQVREI 80
           A+C+M+CE   IL+    QL   +EDP+ ++   ++++L YV+    Y NP +VR+V E 
Sbjct: 89  AECMMDCEAFQILDGIKGQLVGLSEDPSIKIPVSYDRALAYVESCVHYTNPQSVRKVLEP 148

Query: 81  LSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHEDEAIEKMLNDLSLIKKFE 139
           L  Y +++ E+CV+ N   E+V+E  + +PSLKTK  V  ++ ++  L +LS +KK E
Sbjct: 149 LKTYGISDGEMCVIANASSESVDEVLAFIPSLKTKKEV-INQPLQDALEELSKLKKSE 205

The following BLAST results are available for this feature:
BLAST of CsGy5G029380 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O488905.5e-6690.58DNA-directed RNA polymerase II subunit 4 OS=Arabidopsis thaliana OX=3702 GN=NRPB... [more]
O155146.3e-2242.34DNA-directed RNA polymerase II subunit RPB4 OS=Homo sapiens OX=9606 GN=POLR2D PE... [more]
Q9D7M86.3e-2242.34DNA-directed RNA polymerase II subunit RPB4 OS=Mus musculus OX=10090 GN=Polr2d P... [more]
Q54S042.2e-1936.73DNA-directed RNA polymerase II subunit rpb4 OS=Dictyostelium discoideum OX=44689... [more]
Q9VEA54.7e-1738.10DNA-directed RNA polymerase II subunit Rpb4 OS=Drosophila melanogaster OX=7227 G... [more]
BLAST of CsGy5G029380 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004141497.13.54e-90100.00DNA-directed RNA polymerase II subunit 4 [Cucumis sativus] >XP_031742047.1 DNA-d... [more]
XP_008459458.11.44e-8998.55PREDICTED: DNA-directed RNA polymerase II subunit 4-like [Cucumis melo] >XP_0084... [more]
XP_038890773.11.19e-8897.83DNA-directed RNA polymerase II subunit 4-like [Benincasa hispida] >XP_038890774.... [more]
XP_022939401.12.30e-8695.65DNA-directed RNA polymerase II subunit 4-like [Cucurbita moschata] >XP_022939402... [more]
XP_038884885.14.64e-8694.93DNA-directed RNA polymerase II subunit 4 [Benincasa hispida][more]
BLAST of CsGy5G029380 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A1S3CA786.98e-9098.55DNA-directed RNA polymerase II subunit 4-like OS=Cucumis melo OX=3656 GN=LOC1034... [more]
A0A5A7T8116.98e-9098.55DNA-directed RNA polymerase II subunit 4-like OS=Cucumis melo var. makuwa OX=119... [more]
A0A6J1JVN31.11e-8695.65DNA-directed RNA polymerase II subunit 4-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1FFS71.11e-8695.65DNA-directed RNA polymerase II subunit 4-like OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A5D3C0193.19e-8694.20DNA-directed RNA polymerase II subunit 4 OS=Cucumis melo var. makuwa OX=1194695 ... [more]
BLAST of CsGy5G029380 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G09920.13.9e-6790.58RNA polymerase II, Rpb4, core protein [more]
AT4G15950.12.4e-1637.29RNA polymerase II, Rpb4, core protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006590RNA polymerase Rpb4/RPC9, coreSMARTSM00657rpol4neu2coord: 16..138
e-value: 1.3E-56
score: 204.0
IPR038324Rpb4/RPC9 superfamilyGENE3D1.20.1250.40coord: 2..138
e-value: 1.5E-50
score: 172.5
IPR005574RNA polymerase subunit RPB4/RPC9PFAMPF03874RNA_pol_Rpb4coord: 23..132
e-value: 6.0E-24
score: 84.7
NoneNo IPR availablePANTHERPTHR21297DNA-DIRECTED RNA POLYMERASE IIcoord: 1..138
NoneNo IPR availablePANTHERPTHR21297:SF3DNA-DIRECTED RNA POLYMERASE II SUBUNIT 4-LIKEcoord: 1..138
IPR010997HRDC-like superfamilySUPERFAMILY47819HRDC-likecoord: 3..136

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy5G029380.2CsGy5G029380.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0031990 mRNA export from nucleus in response to heat stress
biological_process GO:0000288 nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
biological_process GO:0045948 positive regulation of translational initiation
biological_process GO:0034402 recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex
biological_process GO:0006367 transcription initiation from RNA polymerase II promoter
biological_process GO:0044237 cellular metabolic process
biological_process GO:0006352 DNA-templated transcription, initiation
cellular_component GO:0000932 P-body
cellular_component GO:0005665 RNA polymerase II, core complex
cellular_component GO:0030880 RNA polymerase complex
molecular_function GO:0003899 DNA-directed 5'-3' RNA polymerase activity
molecular_function GO:0000166 nucleotide binding
molecular_function GO:0031369 translation initiation factor binding