CsGy5G029250 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy5G029250
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionTryptophan synthase
LocationGy14Chr5: 32603240 .. 32606555 (+)
RNA-Seq ExpressionCsGy5G029250
SyntenyCsGy5G029250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGCTACCTTCCATGCTGTTGGATCATTAGCTTCTTCAGGCACCAGTAATGTTGGAGGCATTTTCTTTCTCCCCTTTTTCTTTTCTCTATTTTTGTTGTTATTTGTGTTTGGAACTTCAGTTAGTTATGAATGAAATAAACCTGTCATTAGATTAAACCTACAAACTCTTTCTATTTTACTTCTTGTGGATATTGATCATGGTTTCTTTAGTGATTCTGTACCATTTTTTATGCTTCATGAGTTTTTACCTTCAAATTCATCATGTTTTGTTGCCATTTTTGGAGATGAAATTGTAGAAACTGTGTGGGATCTTTCATTTGGACATAACCCAGAAATTTTTTTCTATATAATTTGTGAGATGGGTGATGATAAGAAGGATAATGGAACCTCAGCCAATCTCTGCATTGGTCCACTTGGAAATAAATCCTTGTGGTTTTGCTTTTGAAATTGCCAAGAGGTTTTACACCGATGGGAATAACTGTCTTGACTTAAATGCCATCTATCTCTTCCATATCTAACCAATGTGGACACTATGTTAGTGATATATAATTAAATTTACCTTCACTCACCAGCTTAGGCTTTTGGGTCAATTGGTGATTTAAGAAGGTATTAGAGAGTATGGTCCAAGATGTTTTAAGTTCAAGCTCCTACGTTGTTTCCTCTCATTTAATACTTATTTCCATTTGTTGGGTCTTTCAAATATTTCAAGCCTACAAGTGAGGAGGAGTATTAATGATACATGATTAAATTTACTTCACCCACCAGCTTAAGCTTTTGGGTCAATTGGTGATTTAAAAGTTTGTTTGCATGAGAGAGAGAGATCTGTGTTGAAATGGGATTTGTCTGACTTGATGCTTGAAATGCTCCATTTCTTGTTTTATTGTGAAGCTCTTTGTTGGTTTCGATCAGCCATGTGATTATTGCTGGATTTCACTTTTGCCTTTTTATTTTCTTTTTGGAAGTCTACTGCATTCCTGTCCTTGGTTTCCAATCTTGATAGGAGGAGTTGGCAGTTTTATATTAATAAAGCAGGTCAATTATGTATGGAAAACACTTTCATTCTGACAATGTTGTGATAATTTCCCCATAGATAATAAGCAACTGCCTGGACGTTTTTCACTTAAGAGAACTCCAAGACATTTGAGGTTTTCAAATGGTTATAGAACAAAAGCAGCATCAAATCCTAATTTGAAGTCAGTTGAGGTCCCACGTCAATGGTACAATCTGGTTGCAGATCTTCCTGTAAAACCCCCTCCTCCATTGCATTCCAAGACTCTTGAACCATTAAAACCCGAAGATTTGTCACATCTCTATGCTGATGAATTGATTAAGCAAGAGACCAGTACCGATCGGTACATAAACATCCCCGATGAAGTTGTTGATATTTACGAGCTTTGGCGCCCAACTCCTCTGATCAGGTCTGTGGTATGTCTTGCTAAAATGCATGCATTGCAGCTTTGTTTAACACAACGCTTTCTCATCGTTGTTTGTTATGACATCTTGAATCCTTTCCCAGTAAGTTTTGTTATCAGACTTGCATCTCTTCCTTTTTCCTAAAGAATCTAATGGCATTTTTCAATGTGTATATATGTTAGAGCTAAGAGATTAGAGAAGCTTCTTGACACACCTGCTAGAATTTACTACAAGTATGAAGGAGTTAGCCCTTCTGGGTCACATAAACCCAACTCTGCTGTTCCACAAGTTTGGTATAATGTACAAGAAGGTGTCAAGAATGTTGTTACAGAAACTGGGGCTGGGCAATGGGGATGCAGTTTGGCATTTGCTTGCAGCATATTTGGTATTGGCTGTGAGGTAAGGAAAGATTAGTTGTGAACTTTTTATTTTTTGATATTACTGTTATGAAATCGACAAATTAAGAAAACTAGAAAAGACATTTTAGTGGTTCACCAATTGTGTCTTAACTACATCCATGAGTAGAGGAAGAAATGTTCACTATTAAAGAGAATAATTCAAATTACATATTAGAAGTGCTCCTAGGGTTAGAGAGTTTATATAGCCCACTTCTCTAAGCCCTAGGGTCAGCAAAATGCTTGGTTTGTTCATAAGTGGTAGATAACAGATTCAAAACAAGTAGAAATTTAAAGTTACTTCTAATGATTAACTGTTGTTGAATTAAGATGTAAAAAAAAAAACAGATCAAAGCTCAGCTCCTAACTTACTTCTGAAACATATCACATGAAATTTTATGTTGTGATTTCTTTACAGGTGTGGCAAGTGCGTGCTTCTTATGATCAAAAACCGTATCGACGAATAATGATGGAAACTTGGGGGGCAAAAGTACACCCATCTCCATCCAATATCACAGATGTAGGTAGAAAATTTCTTCAAGTTGACCCCTCAAGTTCGGGAAGTTTGGGAATTGCCATTTCAGAAGCAACGGAGATTGCAACTCTCAATGTTGATACCAGATACTGTTTGGGAAGTGTTCTCAACCATGTTTTGCTTCACCAAACCGTCATCGGTGAAGAGTGTCTAAAACAAATGGAAGCCTTAGGTGAGAGCCCGGATGTTGTAATAGGTTGCACTGGCGGGGGGTCAAACTTCGCAGGTCTTGCCTTCCCCTTCCTCCGCGAGAAACTTGCTGGAAAAATGAACCCTGTTATTAGAGCAGCTGAACCTGCAGCATGTCCTTCGTTAACCAAAGGCGTTTATGCTTATGATTATGGCGATACAGCTGGGATGACACCGTTAATGAAAATGCATACACTTGGTCACAACTTCATTCCTGACCCCATCCACGCTGGTAATGTATAATAAGCTATCAAATGATTGAAACTAATTGTTTCTCTTGATCATCATCTTGGCTCACTCGTTTTTGGCAGGTGGATTGCGGTATCATGGTATTGCTCCATTGATCTCACATGTCTATAATCTTGGTCTTTTGGAAGCTATATCACTTCCCCAAACAGAATGCTTTCGAGGTACCGATACATACTCAAATTTATTAAAGTCAACCGAAAAATAAACCAGTTACTAGAAATTTGCCTCTGCATGACCGGCCAACAATGCAACAACTAAGTTTCTTTTTGTTTGTAGGGGCTGTACAATTTGCTCGATCGGAAGGACTAATACCAGCACCTGAACCAACTCACGCAATTGCTGCAACTATCCGAGAGGCTCTTCGCTGTCGAGACACCGGAGAGTCGAAAGTTATTCTCACAGCCATGAGTGGGCATGGTCACTTTGACTTGCCAGTGTACGAGAAGTATCTACAAGGTGGGATTAGAGATCTTTCTTTTGCAAAGGAGAAGATACAAGAATCATTAACATCCATTCCTCAGGTGGCCTGA

mRNA sequence

ATGGCCGCTACCTTCCATGCTGTTGGATCATTAGCTTCTTCAGGCACCAGTAATGTTGGAGATAATAAGCAACTGCCTGGACGTTTTTCACTTAAGAGAACTCCAAGACATTTGAGGTTTTCAAATGGTTATAGAACAAAAGCAGCATCAAATCCTAATTTGAAGTCAGTTGAGGTCCCACGTCAATGGTACAATCTGGTTGCAGATCTTCCTGTAAAACCCCCTCCTCCATTGCATTCCAAGACTCTTGAACCATTAAAACCCGAAGATTTGTCACATCTCTATGCTGATGAATTGATTAAGCAAGAGACCAGTACCGATCGGTACATAAACATCCCCGATGAAGTTGTTGATATTTACGAGCTTTGGCGCCCAACTCCTCTGATCAGAGCTAAGAGATTAGAGAAGCTTCTTGACACACCTGCTAGAATTTACTACAAGTATGAAGGAGTTAGCCCTTCTGGGTCACATAAACCCAACTCTGCTGTTCCACAAGTTTGGTATAATGTACAAGAAGGTGTCAAGAATGTTGTTACAGAAACTGGGGCTGGGCAATGGGGATGCAGTTTGGCATTTGCTTGCAGCATATTTGGTATTGGCTGTGAGGTGTGGCAAGTGCGTGCTTCTTATGATCAAAAACCGTATCGACGAATAATGATGGAAACTTGGGGGGCAAAAGTACACCCATCTCCATCCAATATCACAGATGTAGGTAGAAAATTTCTTCAAGTTGACCCCTCAAGTTCGGGAAGTTTGGGAATTGCCATTTCAGAAGCAACGGAGATTGCAACTCTCAATGTTGATACCAGATACTGTTTGGGAAGTGTTCTCAACCATGTTTTGCTTCACCAAACCGTCATCGGTGAAGAGTGTCTAAAACAAATGGAAGCCTTAGGTGAGAGCCCGGATGTTGTAATAGGTTGCACTGGCGGGGGGTCAAACTTCGCAGGTCTTGCCTTCCCCTTCCTCCGCGAGAAACTTGCTGGAAAAATGAACCCTGTTATTAGAGCAGCTGAACCTGCAGCATGTCCTTCGTTAACCAAAGGCGTTTATGCTTATGATTATGGCGATACAGCTGGGATGACACCGTTAATGAAAATGCATACACTTGGTCACAACTTCATTCCTGACCCCATCCACGCTGGTGGATTGCGGTATCATGGTATTGCTCCATTGATCTCACATGTCTATAATCTTGGTCTTTTGGAAGCTATATCACTTCCCCAAACAGAATGCTTTCGAGGGGCTGTACAATTTGCTCGATCGGAAGGACTAATACCAGCACCTGAACCAACTCACGCAATTGCTGCAACTATCCGAGAGGCTCTTCGCTGTCGAGACACCGGAGAGTCGAAAGTTATTCTCACAGCCATGAGTGGGCATGGTCACTTTGACTTGCCAGTGTACGAGAAGTATCTACAAGGTGGGATTAGAGATCTTTCTTTTGCAAAGGAGAAGATACAAGAATCATTAACATCCATTCCTCAGGTGGCCTGA

Coding sequence (CDS)

ATGGCCGCTACCTTCCATGCTGTTGGATCATTAGCTTCTTCAGGCACCAGTAATGTTGGAGATAATAAGCAACTGCCTGGACGTTTTTCACTTAAGAGAACTCCAAGACATTTGAGGTTTTCAAATGGTTATAGAACAAAAGCAGCATCAAATCCTAATTTGAAGTCAGTTGAGGTCCCACGTCAATGGTACAATCTGGTTGCAGATCTTCCTGTAAAACCCCCTCCTCCATTGCATTCCAAGACTCTTGAACCATTAAAACCCGAAGATTTGTCACATCTCTATGCTGATGAATTGATTAAGCAAGAGACCAGTACCGATCGGTACATAAACATCCCCGATGAAGTTGTTGATATTTACGAGCTTTGGCGCCCAACTCCTCTGATCAGAGCTAAGAGATTAGAGAAGCTTCTTGACACACCTGCTAGAATTTACTACAAGTATGAAGGAGTTAGCCCTTCTGGGTCACATAAACCCAACTCTGCTGTTCCACAAGTTTGGTATAATGTACAAGAAGGTGTCAAGAATGTTGTTACAGAAACTGGGGCTGGGCAATGGGGATGCAGTTTGGCATTTGCTTGCAGCATATTTGGTATTGGCTGTGAGGTGTGGCAAGTGCGTGCTTCTTATGATCAAAAACCGTATCGACGAATAATGATGGAAACTTGGGGGGCAAAAGTACACCCATCTCCATCCAATATCACAGATGTAGGTAGAAAATTTCTTCAAGTTGACCCCTCAAGTTCGGGAAGTTTGGGAATTGCCATTTCAGAAGCAACGGAGATTGCAACTCTCAATGTTGATACCAGATACTGTTTGGGAAGTGTTCTCAACCATGTTTTGCTTCACCAAACCGTCATCGGTGAAGAGTGTCTAAAACAAATGGAAGCCTTAGGTGAGAGCCCGGATGTTGTAATAGGTTGCACTGGCGGGGGGTCAAACTTCGCAGGTCTTGCCTTCCCCTTCCTCCGCGAGAAACTTGCTGGAAAAATGAACCCTGTTATTAGAGCAGCTGAACCTGCAGCATGTCCTTCGTTAACCAAAGGCGTTTATGCTTATGATTATGGCGATACAGCTGGGATGACACCGTTAATGAAAATGCATACACTTGGTCACAACTTCATTCCTGACCCCATCCACGCTGGTGGATTGCGGTATCATGGTATTGCTCCATTGATCTCACATGTCTATAATCTTGGTCTTTTGGAAGCTATATCACTTCCCCAAACAGAATGCTTTCGAGGGGCTGTACAATTTGCTCGATCGGAAGGACTAATACCAGCACCTGAACCAACTCACGCAATTGCTGCAACTATCCGAGAGGCTCTTCGCTGTCGAGACACCGGAGAGTCGAAAGTTATTCTCACAGCCATGAGTGGGCATGGTCACTTTGACTTGCCAGTGTACGAGAAGTATCTACAAGGTGGGATTAGAGATCTTTCTTTTGCAAAGGAGAAGATACAAGAATCATTAACATCCATTCCTCAGGTGGCCTGA

Protein sequence

MAATFHAVGSLASSGTSNVGDNKQLPGRFSLKRTPRHLRFSNGYRTKAASNPNLKSVEVPRQWYNLVADLPVKPPPPLHSKTLEPLKPEDLSHLYADELIKQETSTDRYINIPDEVVDIYELWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPSGSHKPNSAVPQVWYNVQEGVKNVVTETGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRIMMETWGAKVHPSPSNITDVGRKFLQVDPSSSGSLGIAISEATEIATLNVDTRYCLGSVLNHVLLHQTVIGEECLKQMEALGESPDVVIGCTGGGSNFAGLAFPFLREKLAGKMNPVIRAAEPAACPSLTKGVYAYDYGDTAGMTPLMKMHTLGHNFIPDPIHAGGLRYHGIAPLISHVYNLGLLEAISLPQTECFRGAVQFARSEGLIPAPEPTHAIAATIREALRCRDTGESKVILTAMSGHGHFDLPVYEKYLQGGIRDLSFAKEKIQESLTSIPQVA*
Homology
BLAST of CsGy5G029250 vs. ExPASy Swiss-Prot
Match: O67409 (Tryptophan synthase beta chain 2 OS=Aquifex aeolicus (strain VF5) OX=224324 GN=trpB2 PE=3 SV=1)

HSP 1 Score: 531.9 bits (1369), Expect = 7.5e-150
Identity = 254/429 (59.21%), Postives = 318/429 (74.13%), Query Frame = 0

Query: 54  LKSVEVPRQWYNLVADLPVKPPPPLHSKTLEPLKPEDLSHLYADELIKQETSTDRYINIP 113
           L   E+P++W N++  LP    PPL  +T+EP+KPE L  ++ + L++QE S   +I+IP
Sbjct: 6   LSEGEIPKKWLNILPLLPEPLEPPLDPETMEPVKPEKLLAIFPEPLVEQEVSDKEWIDIP 65

Query: 114 DEVVDIYELWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPSGSHKPNSAVPQVWYNVQEG 173
           +EV+DIY LWRPTPL RAK LE+ L TPA+I+YK E VSP GSHKPN+AV Q +YN   G
Sbjct: 66  EEVLDIYSLWRPTPLHRAKNLEEFLGTPAKIFYKNESVSPPGSHKPNTAVAQAYYNKISG 125

Query: 174 VKNVVTETGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRIMMETWGAKVHPSPSN 233
           VK + TETGAGQWG +L+FA   F + C V+ VR SY+QKPYRRI+METW  +V PSPS 
Sbjct: 126 VKRLTTETGAGQWGSALSFATQFFDLQCRVYMVRVSYNQKPYRRILMETWKGEVIPSPSP 185

Query: 234 ITDVGRKFLQVDPSSSGSLGIAISEATEIATLNVDTRYCLGSVLNHVLLHQTVIGEECLK 293
            T+ GRK+ + +P   GSLGIAISEA E A    DT+Y LGSVLNHVLLHQTVIG E  K
Sbjct: 186 YTNAGRKYYEENPEHPGSLGIAISEAIEEAASREDTKYSLGSVLNHVLLHQTVIGLEAKK 245

Query: 294 QMEALGESPDVVIGCTGGGSNFAGLAFPFLREKLAG---KMNPVIRAAEPAACPSLTKGV 353
           QME  G  PDV+IG  GGGSNFAGL+FPFL + L G   K +  + A EP ACP+LTKG 
Sbjct: 246 QMEEAGYYPDVIIGAVGGGSNFAGLSFPFLADVLRGDKRKEDLKVLAVEPEACPTLTKGE 305

Query: 354 YAYDYGDTAGMTPLMKMHTLGHNFIPDPIHAGGLRYHGIAPLISHVYNLGLLEAISLPQT 413
           Y YD+GD+ G+TPL+KM+TLGH+F+P PIHAGGLRYHG APL+  +YNLG ++A++  QT
Sbjct: 306 YKYDFGDSVGLTPLIKMYTLGHDFVPSPIHAGGLRYHGDAPLVCKLYNLGYIDAVAYKQT 365

Query: 414 ECFRGAVQFARSEGLIPAPEPTHAIAATIREALRCRDTGESKVILTAMSGHGHFDLPVYE 473
           E F  AV FAR+EG++PAPE  HAI A I EAL+C++TGE KVIL  +SGHG+FDL  Y+
Sbjct: 366 EVFEAAVTFARTEGIVPAPESAHAIKAAIDEALKCKETGEEKVILFNLSGHGYFDLSAYD 425

Query: 474 KYLQGGIRD 480
           KYL G + D
Sbjct: 426 KYLHGELTD 434

BLAST of CsGy5G029250 vs. ExPASy Swiss-Prot
Match: Q8Q001 (Tryptophan synthase beta chain 2 OS=Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) OX=192952 GN=trpB2 PE=3 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 1.6e-147
Identity = 245/418 (58.61%), Postives = 315/418 (75.36%), Query Frame = 0

Query: 58  EVPRQWYNLVADLPVKPPPPLHSKTLEPLKPEDLSHLYADELIKQETSTDRYINIPDEVV 117
           E+P++WYN+++DLP    PPL  +T +P+ PE L  ++A ELI+QE S+DRYI+IP E++
Sbjct: 22  EMPKRWYNVLSDLPSPIDPPLDPRTWQPINPEALEPIFAKELIRQEMSSDRYIDIPAEIL 81

Query: 118 DIYELWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPSGSHKPNSAVPQVWYNVQEGVKNV 177
           D+Y LWRP+PL RA +LEK L TPA+IYYKYEGVSP+GSHK N+++ Q +YN++EG + +
Sbjct: 82  DVYRLWRPSPLFRAHQLEKDLKTPAKIYYKYEGVSPAGSHKTNTSIAQAYYNMKEGTERL 141

Query: 178 VTETGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRIMMETWGAKVHPSPSNITDV 237
            TETGAGQWG +L+ AC+ F + C+V+ VR+SY QKPYR+ +M  WG  V PSPS  T+ 
Sbjct: 142 TTETGAGQWGSALSLACNYFDLECKVYMVRSSYYQKPYRKSLMTLWGGNVVPSPSPDTEF 201

Query: 238 GRKFLQVDPSSSGSLGIAISEATEIATLNVDTRYCLGSVLNHVLLHQTVIGEECLKQMEA 297
           GRK L+  P + GSLGIAISEA E A  + +T+Y LGSVLNHV+LHQTVIG EC KQ+E 
Sbjct: 202 GRKILKEQPDTPGSLGIAISEAVEDAIAHDNTKYTLGSVLNHVVLHQTVIGAECKKQLEQ 261

Query: 298 LGESPDVVIGCTGGGSNFAGLAFPFLREKLAGKMNPVIRAAEPAACPSLTKGVYAYDYGD 357
           + E PDVVIGC GGGSN  G+   F++++L GK N  + A EP+ACPSLTKG Y YD+GD
Sbjct: 262 VEEYPDVVIGCCGGGSNLGGIGLEFIKDRLEGKHNARVVAVEPSACPSLTKGEYRYDFGD 321

Query: 358 TAGMTPLMKMHTLGHNFIPDPIHAGGLRYHGIAPLISHVYNLGLLEAISLPQTECFRGAV 417
           TA MTPL+KM+TLGH  IP  IHAGGLRYHG +P+IS +   GL+EA+S  Q E F  AV
Sbjct: 322 TAEMTPLLKMYTLGHKHIPPAIHAGGLRYHGDSPIISKLCAEGLMEAVSYGQKEVFDAAV 381

Query: 418 QFARSEGLIPAPEPTHAIAATIREALRCRDTGESKVILTAMSGHGHFDLPVYEKYLQG 476
           QFAR+EG++PAPE +HAI   I EAL  +  GE KVIL  +SGHGHFD+  Y+KY  G
Sbjct: 382 QFARTEGIVPAPESSHAIRCAIDEALEAKQKGEEKVILFNLSGHGHFDMASYDKYFSG 439

BLAST of CsGy5G029250 vs. ExPASy Swiss-Prot
Match: O29028 (Tryptophan synthase beta chain 2 OS=Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) OX=224325 GN=trpB2 PE=3 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 5.0e-146
Identity = 246/426 (57.75%), Postives = 313/426 (73.47%), Query Frame = 0

Query: 54  LKSVEVPRQWYNLVADLPVKPPPPLHSKTLEPLKPEDLSHLYADELIKQETSTDRYINIP 113
           L   E+P++WYN++ DLP   PPPLH  T EP+KPEDL  ++   LI+QE S +R+I IP
Sbjct: 11  LDESEMPKEWYNVLPDLPEPLPPPLHPATQEPVKPEDLEPIFPKGLIQQEMSGERWIRIP 70

Query: 114 DEVVDIYELWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPSGSHKPNSAVPQVWYNVQEG 173
           ++V +IY +WRPTPL+RA+RLEK L TPARIY+KYEG SP GSHKPN+AV Q +YN +EG
Sbjct: 71  EDVREIYRIWRPTPLVRAERLEKALKTPARIYFKYEGASPPGSHKPNTAVAQAYYNAKEG 130

Query: 174 VKNVVTETGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRIMMETWGAKVHPSPSN 233
           V+ + TETGAGQWG +L FA  +F + C V+ V+ S+ QKPYRR+MMETWG +V PSPS+
Sbjct: 131 VERLTTETGAGQWGSALCFATKLFEMACTVYMVKVSFMQKPYRRVMMETWGGEVIPSPSD 190

Query: 234 ITDVGRKFLQVDPSSSGSLGIAISEATEIATLNVDTRYCLGSVLNHVLLHQTVIGEECLK 293
            T+VGRK L  +P + GSLGIAISEA E A  N +T+Y LGSVLNHVLLHQTVIG E   
Sbjct: 191 RTEVGRKILAENPDTPGSLGIAISEAIEDAAKNENTKYSLGSVLNHVLLHQTVIGLETKA 250

Query: 294 QMEALGESPDVVIGCTGGGSNFAGLAFPFLREKLAGKMNPVIRAAEPAACPSLTKGVYAY 353
           Q+E + E PDV+IGC GGGSNFAGL +PF+ +   G +   I A EPAACP+LT G Y Y
Sbjct: 251 QLEKVDEKPDVLIGCVGGGSNFAGLTYPFVNDAKNGDLE--IIAVEPAACPTLTAGEYKY 310

Query: 354 DYGDTAGMTPLMKMHTLGHNFIPDPIHAGGLRYHGIAPLISHVYNLGLLEAISLPQTECF 413
           D+GD AG+TPL+KM+TLGH+FIP PIHAGGLRYHG AP +  +   G+++A ++ Q   F
Sbjct: 311 DFGDVAGLTPLLKMYTLGHDFIPPPIHAGGLRYHGDAPTLCMLVKHGVIKARAVKQLPTF 370

Query: 414 RGAVQFARSEGLIPAPEPTHAIAATIREALRCRDTGESKVILTAMSGHGHFDLPVYEKYL 473
              + FAR+EG+IPAPE  HA+ A I EA++ R+  E KVI+   SGHG  DL  Y+ YL
Sbjct: 371 EAGLLFARTEGIIPAPETNHAVRAAIDEAIKARENNEEKVIVFGFSGHGLLDLQAYDDYL 430

Query: 474 QGGIRD 480
            G + D
Sbjct: 431 AGRLAD 434

BLAST of CsGy5G029250 vs. ExPASy Swiss-Prot
Match: Q8TL44 (Tryptophan synthase beta chain 2 OS=Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) OX=188937 GN=trpB2 PE=3 SV=1)

HSP 1 Score: 515.8 bits (1327), Expect = 5.6e-145
Identity = 238/418 (56.94%), Postives = 314/418 (75.12%), Query Frame = 0

Query: 58  EVPRQWYNLVADLPVKPPPPLHSKTLEPLKPEDLSHLYADELIKQETSTDRYINIPDEVV 117
           E+P++WYN++ADLP    PPL  +T +P+ P+ L  ++   LI QE S+DRYI+IP+EV+
Sbjct: 22  EMPKKWYNVLADLPSPIDPPLDPRTWQPISPDALEPIFPKALIMQEMSSDRYIDIPEEVL 81

Query: 118 DIYELWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPSGSHKPNSAVPQVWYNVQEGVKNV 177
           D+Y LWRP+PL RA +LEK+L +PA+IYYKYEGVSP+GSHK N+++ Q +YN++EG + +
Sbjct: 82  DVYRLWRPSPLFRAHQLEKVLKSPAKIYYKYEGVSPAGSHKTNTSIAQAYYNMKEGTERL 141

Query: 178 VTETGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRIMMETWGAKVHPSPSNITDV 237
            TETGAGQWG +L+ AC+ F + C+V+ VR+S+ QKPYR+ ++  WG  V PSPS  T+ 
Sbjct: 142 TTETGAGQWGSALSLACNYFDLECKVYMVRSSFYQKPYRKSLITLWGGNVVPSPSPDTEF 201

Query: 238 GRKFLQVDPSSSGSLGIAISEATEIATLNVDTRYCLGSVLNHVLLHQTVIGEECLKQMEA 297
           GRK LQ  P + GSLGIAISEA E A  + +T+Y LGSVLNHV+LHQTVIG EC +Q+  
Sbjct: 202 GRKILQEQPDTPGSLGIAISEAVEDAIAHENTKYSLGSVLNHVVLHQTVIGAECKQQLAQ 261

Query: 298 LGESPDVVIGCTGGGSNFAGLAFPFLREKLAGKMNPVIRAAEPAACPSLTKGVYAYDYGD 357
           + E PDVVIGC GGGSN  G+   F++++L GK +  + A EP+ACPSLTKG Y YD+GD
Sbjct: 262 VEEYPDVVIGCCGGGSNLGGIGLEFIKDRLEGKHSARVVAVEPSACPSLTKGEYRYDFGD 321

Query: 358 TAGMTPLMKMHTLGHNFIPDPIHAGGLRYHGIAPLISHVYNLGLLEAISLPQTECFRGAV 417
           TA MTPL+KM+TLGH  +P  IHAGGLRYHG +P+IS + + GL+EA+S  Q E F  AV
Sbjct: 322 TAEMTPLLKMYTLGHKHVPPAIHAGGLRYHGDSPIISKLCSEGLMEAVSYDQQEVFDAAV 381

Query: 418 QFARSEGLIPAPEPTHAIAATIREALRCRDTGESKVILTAMSGHGHFDLPVYEKYLQG 476
           QFAR+EG++PAPE +HAI   I EAL  + TGE K IL  +SGHGHFD+  Y+KY  G
Sbjct: 382 QFARTEGIVPAPESSHAIRCAIDEALAAKQTGEEKTILFNLSGHGHFDMSSYDKYFSG 439

BLAST of CsGy5G029250 vs. ExPASy Swiss-Prot
Match: Q9WZ09 (Tryptophan synthase beta chain 2 OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=trpB2 PE=3 SV=1)

HSP 1 Score: 495.4 bits (1274), Expect = 7.8e-139
Identity = 241/417 (57.79%), Postives = 308/417 (73.86%), Query Frame = 0

Query: 53  NLKSVEVPRQWYNLVADLPVKPPPPLHSKTLEPLKPEDLSHLYADELIKQETSTDRYINI 112
           NLK  E+P+ WYN++ADLP K  PPL  +T +P+ PE LS ++   LI+QE S +R+I I
Sbjct: 6   NLKPEEIPKHWYNVLADLPFKLDPPLDPETKQPISPEKLSVIFPMSLIEQEVSEERFIEI 65

Query: 113 PDEVVDIYELWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPSGSHKPNSAVPQVWYNVQE 172
           P+ V+  Y ++RPTPLIRA  LE+ L TPARIYYKYEGVSP+GSHKPN+A+ Q +YN  E
Sbjct: 66  PEPVLKEYAVYRPTPLIRATFLEEYLQTPARIYYKYEGVSPTGSHKPNTAIAQAYYNKIE 125

Query: 173 GVKNVVTETGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRIMMETWGAKVHPSPS 232
           GVK +VTETGAGQWG +L++A + FG+  +V+ V+ SY QKP R+ MM  +G KV PSPS
Sbjct: 126 GVKRLVTETGAGQWGSALSYAGAKFGLEVKVFMVKVSYQQKPMRKYMMNLFGGKVTPSPS 185

Query: 233 NITDVGRKFLQVDPSSSGSLGIAISEATEIATLNVDTRYCLGSVLNHVLLHQTVIGEECL 292
             T+ GRK L  DP + GSLGIAISEA E+A  + +T+Y LGSVLNHVLLHQTVIG E  
Sbjct: 186 EETNFGRKILSEDPDNPGSLGIAISEALEVAVSDPNTKYSLGSVLNHVLLHQTVIGLEIK 245

Query: 293 KQMEALGESPDVVIGCTGGGSNFAGLAFPFLREKLAGKMNPVIRAAEPAACPSLTKGVYA 352
           KQ+E +GE PD+++GC GGGSNF G   PF+ +KL+G+    + A EPAACPSLTKG Y 
Sbjct: 246 KQLELIGEKPDILLGCHGGGSNFGGTILPFVPDKLSGRDIRFV-ACEPAACPSLTKGNYD 305

Query: 353 YDYGDTAGMTPLMKMHTLGHNFIPDPIHAGGLRYHGIAPLISHVYNLGLLEAISLPQTEC 412
           YD+GDTAG+TPL+KM+TLG +FIP  IHAGGLRYHG AP+I+ +   GL+EA +  Q E 
Sbjct: 306 YDFGDTAGLTPLLKMYTLGKDFIPPKIHAGGLRYHGSAPIIARLVKEGLVEAQAFDQDET 365

Query: 413 FRGAVQFARSEGLIPAPEPTHAIAATIREALRCRDTGESKVILTAMSGHGHFDLPVY 470
           F  A  FA+ EG+IPAPE  HAIA  IREA + ++ G+ +VI+  +SGHG  DL  Y
Sbjct: 366 FEAAKIFAKLEGIIPAPESAHAIAGAIREAKKAKEEGKERVIVFTLSGHGLLDLTAY 421

BLAST of CsGy5G029250 vs. NCBI nr
Match: XP_011656025.2 (uncharacterized protein LOC101219882 isoform X1 [Cucumis sativus] >XP_011656026.2 uncharacterized protein LOC101219882 isoform X1 [Cucumis sativus] >XP_031742107.1 uncharacterized protein LOC101219882 isoform X1 [Cucumis sativus])

HSP 1 Score: 1014 bits (2622), Expect = 0.0
Identity = 498/498 (100.00%), Postives = 498/498 (100.00%), Query Frame = 0

Query: 1   MAATFHAVGSLASSGTSNVGDNKQLPGRFSLKRTPRHLRFSNGYRTKAASNPNLKSVEVP 60
           MAATFHAVGSLASSGTSNVGDNKQLPGRFSLKRTPRHLRFSNGYRTKAASNPNLKSVEVP
Sbjct: 1   MAATFHAVGSLASSGTSNVGDNKQLPGRFSLKRTPRHLRFSNGYRTKAASNPNLKSVEVP 60

Query: 61  RQWYNLVADLPVKPPPPLHSKTLEPLKPEDLSHLYADELIKQETSTDRYINIPDEVVDIY 120
           RQWYNLVADLPVKPPPPLHSKTLEPLKPEDLSHLYADELIKQETSTDRYINIPDEVVDIY
Sbjct: 61  RQWYNLVADLPVKPPPPLHSKTLEPLKPEDLSHLYADELIKQETSTDRYINIPDEVVDIY 120

Query: 121 ELWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPSGSHKPNSAVPQVWYNVQEGVKNVVTE 180
           ELWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPSGSHKPNSAVPQVWYNVQEGVKNVVTE
Sbjct: 121 ELWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPSGSHKPNSAVPQVWYNVQEGVKNVVTE 180

Query: 181 TGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRIMMETWGAKVHPSPSNITDVGRK 240
           TGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRIMMETWGAKVHPSPSNITDVGRK
Sbjct: 181 TGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRIMMETWGAKVHPSPSNITDVGRK 240

Query: 241 FLQVDPSSSGSLGIAISEATEIATLNVDTRYCLGSVLNHVLLHQTVIGEECLKQMEALGE 300
           FLQVDPSSSGSLGIAISEATEIATLNVDTRYCLGSVLNHVLLHQTVIGEECLKQMEALGE
Sbjct: 241 FLQVDPSSSGSLGIAISEATEIATLNVDTRYCLGSVLNHVLLHQTVIGEECLKQMEALGE 300

Query: 301 SPDVVIGCTGGGSNFAGLAFPFLREKLAGKMNPVIRAAEPAACPSLTKGVYAYDYGDTAG 360
           SPDVVIGCTGGGSNFAGLAFPFLREKLAGKMNPVIRAAEPAACPSLTKGVYAYDYGDTAG
Sbjct: 301 SPDVVIGCTGGGSNFAGLAFPFLREKLAGKMNPVIRAAEPAACPSLTKGVYAYDYGDTAG 360

Query: 361 MTPLMKMHTLGHNFIPDPIHAGGLRYHGIAPLISHVYNLGLLEAISLPQTECFRGAVQFA 420
           MTPLMKMHTLGHNFIPDPIHAGGLRYHGIAPLISHVYNLGLLEAISLPQTECFRGAVQFA
Sbjct: 361 MTPLMKMHTLGHNFIPDPIHAGGLRYHGIAPLISHVYNLGLLEAISLPQTECFRGAVQFA 420

Query: 421 RSEGLIPAPEPTHAIAATIREALRCRDTGESKVILTAMSGHGHFDLPVYEKYLQGGIRDL 480
           RSEGLIPAPEPTHAIAATIREALRCRDTGESKVILTAMSGHGHFDLPVYEKYLQGGIRDL
Sbjct: 421 RSEGLIPAPEPTHAIAATIREALRCRDTGESKVILTAMSGHGHFDLPVYEKYLQGGIRDL 480

Query: 481 SFAKEKIQESLTSIPQVA 498
           SFAKEKIQESLTSIPQVA
Sbjct: 481 SFAKEKIQESLTSIPQVA 498

BLAST of CsGy5G029250 vs. NCBI nr
Match: XP_031742108.1 (uncharacterized protein LOC101219882 isoform X2 [Cucumis sativus] >XP_031742109.1 uncharacterized protein LOC101219882 isoform X2 [Cucumis sativus])

HSP 1 Score: 999 bits (2582), Expect = 0.0
Identity = 493/498 (99.00%), Postives = 494/498 (99.20%), Query Frame = 0

Query: 1   MAATFHAVGSLASSGTSNVGDNKQLPGRFSLKRTPRHLRFSNGYRTKAASNPNLKSVEVP 60
           MAATFHAVGSLASSGT+N    KQLPGRFSLKRTPRHLRFSNGYRTKAASNPNLKSVEVP
Sbjct: 1   MAATFHAVGSLASSGTNN----KQLPGRFSLKRTPRHLRFSNGYRTKAASNPNLKSVEVP 60

Query: 61  RQWYNLVADLPVKPPPPLHSKTLEPLKPEDLSHLYADELIKQETSTDRYINIPDEVVDIY 120
           RQWYNLVADLPVKPPPPLHSKTLEPLKPEDLSHLYADELIKQETSTDRYINIPDEVVDIY
Sbjct: 61  RQWYNLVADLPVKPPPPLHSKTLEPLKPEDLSHLYADELIKQETSTDRYINIPDEVVDIY 120

Query: 121 ELWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPSGSHKPNSAVPQVWYNVQEGVKNVVTE 180
           ELWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPSGSHKPNSAVPQVWYNVQEGVKNVVTE
Sbjct: 121 ELWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPSGSHKPNSAVPQVWYNVQEGVKNVVTE 180

Query: 181 TGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRIMMETWGAKVHPSPSNITDVGRK 240
           TGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRIMMETWGAKVHPSPSNITDVGRK
Sbjct: 181 TGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRIMMETWGAKVHPSPSNITDVGRK 240

Query: 241 FLQVDPSSSGSLGIAISEATEIATLNVDTRYCLGSVLNHVLLHQTVIGEECLKQMEALGE 300
           FLQVDPSSSGSLGIAISEATEIATLNVDTRYCLGSVLNHVLLHQTVIGEECLKQMEALGE
Sbjct: 241 FLQVDPSSSGSLGIAISEATEIATLNVDTRYCLGSVLNHVLLHQTVIGEECLKQMEALGE 300

Query: 301 SPDVVIGCTGGGSNFAGLAFPFLREKLAGKMNPVIRAAEPAACPSLTKGVYAYDYGDTAG 360
           SPDVVIGCTGGGSNFAGLAFPFLREKLAGKMNPVIRAAEPAACPSLTKGVYAYDYGDTAG
Sbjct: 301 SPDVVIGCTGGGSNFAGLAFPFLREKLAGKMNPVIRAAEPAACPSLTKGVYAYDYGDTAG 360

Query: 361 MTPLMKMHTLGHNFIPDPIHAGGLRYHGIAPLISHVYNLGLLEAISLPQTECFRGAVQFA 420
           MTPLMKMHTLGHNFIPDPIHAGGLRYHGIAPLISHVYNLGLLEAISLPQTECFRGAVQFA
Sbjct: 361 MTPLMKMHTLGHNFIPDPIHAGGLRYHGIAPLISHVYNLGLLEAISLPQTECFRGAVQFA 420

Query: 421 RSEGLIPAPEPTHAIAATIREALRCRDTGESKVILTAMSGHGHFDLPVYEKYLQGGIRDL 480
           RSEGLIPAPEPTHAIAATIREALRCRDTGESKVILTAMSGHGHFDLPVYEKYLQGGIRDL
Sbjct: 421 RSEGLIPAPEPTHAIAATIREALRCRDTGESKVILTAMSGHGHFDLPVYEKYLQGGIRDL 480

Query: 481 SFAKEKIQESLTSIPQVA 498
           SFAKEKIQESLTSIPQVA
Sbjct: 481 SFAKEKIQESLTSIPQVA 494

BLAST of CsGy5G029250 vs. NCBI nr
Match: XP_008459435.1 (PREDICTED: tryptophan synthase beta chain 2-like isoform X2 [Cucumis melo] >XP_016902452.1 PREDICTED: tryptophan synthase beta chain 2-like isoform X2 [Cucumis melo])

HSP 1 Score: 971 bits (2509), Expect = 0.0
Identity = 478/498 (95.98%), Postives = 487/498 (97.79%), Query Frame = 0

Query: 1   MAATFHAVGSLASSGTSNVGDNKQLPGRFSLKRTPRHLRFSNGYRTKAASNPNLKSVEVP 60
           M+ATFHAVGSL+SSG SN GD +QLPGR S  RTPRHLRFSNGYRTKAASN NLKSVEVP
Sbjct: 1   MSATFHAVGSLSSSGISNGGDIEQLPGRLS-PRTPRHLRFSNGYRTKAASNLNLKSVEVP 60

Query: 61  RQWYNLVADLPVKPPPPLHSKTLEPLKPEDLSHLYADELIKQETSTDRYINIPDEVVDIY 120
           RQWYNLVADLPVKPPPPLHSKTLEPLKPEDLSH YADELIKQETSTDRYINIPDEVVDIY
Sbjct: 61  RQWYNLVADLPVKPPPPLHSKTLEPLKPEDLSHFYADELIKQETSTDRYINIPDEVVDIY 120

Query: 121 ELWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPSGSHKPNSAVPQVWYNVQEGVKNVVTE 180
           +LWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPSGSHKPNSAVPQVWYNVQEGVKNVVTE
Sbjct: 121 KLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPSGSHKPNSAVPQVWYNVQEGVKNVVTE 180

Query: 181 TGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRIMMETWGAKVHPSPSNITDVGRK 240
           TGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRIMMETWGAKVHPSPSNITDVGRK
Sbjct: 181 TGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRIMMETWGAKVHPSPSNITDVGRK 240

Query: 241 FLQVDPSSSGSLGIAISEATEIATLNVDTRYCLGSVLNHVLLHQTVIGEECLKQMEALGE 300
           FLQVDPSSSGSLGIAISEATE+ATLNVDTRYCLGSVLNHVLLHQTVIGEECLKQMEALGE
Sbjct: 241 FLQVDPSSSGSLGIAISEATEVATLNVDTRYCLGSVLNHVLLHQTVIGEECLKQMEALGE 300

Query: 301 SPDVVIGCTGGGSNFAGLAFPFLREKLAGKMNPVIRAAEPAACPSLTKGVYAYDYGDTAG 360
           +PDVVIGCTGGGSNFAGL FPFLREKLAGKMNPVIRAAEPAACPSLTKGVYAYDYGDTAG
Sbjct: 301 NPDVVIGCTGGGSNFAGLVFPFLREKLAGKMNPVIRAAEPAACPSLTKGVYAYDYGDTAG 360

Query: 361 MTPLMKMHTLGHNFIPDPIHAGGLRYHGIAPLISHVYNLGLLEAISLPQTECFRGAVQFA 420
           MTPLMKMHTLGHNFIPDPIHAGGLRYHGIAPLISHV NLGLLEAISLPQTECFRGAVQFA
Sbjct: 361 MTPLMKMHTLGHNFIPDPIHAGGLRYHGIAPLISHVCNLGLLEAISLPQTECFRGAVQFA 420

Query: 421 RSEGLIPAPEPTHAIAATIREALRCRDTGESKVILTAMSGHGHFDLPVYEKYLQGGIRDL 480
           RSEGLIPAPEPTHAIAATIREALRCR+TGESKVILTAMSGHGHFDLPVYEKYL+GGI DL
Sbjct: 421 RSEGLIPAPEPTHAIAATIREALRCRETGESKVILTAMSGHGHFDLPVYEKYLRGGITDL 480

Query: 481 SFAKEKIQESLTSIPQVA 498
           SFAKEKIQESLT+IPQVA
Sbjct: 481 SFAKEKIQESLTTIPQVA 497

BLAST of CsGy5G029250 vs. NCBI nr
Match: KAE8648956.1 (hypothetical protein Csa_007904 [Cucumis sativus])

HSP 1 Score: 962 bits (2488), Expect = 0.0
Identity = 474/498 (95.18%), Postives = 481/498 (96.59%), Query Frame = 0

Query: 1   MAATFHAVGSLASSGTSNVGDNKQLPGRFSLKRTPRHLRFSNGYRTKAASNPNLKSVEVP 60
           MAATF  VGSL SS  +NVG+  QLPG  SLKR P HLRFSNGYRTKAASNPNLK+VEVP
Sbjct: 1   MAATFLTVGSLTSSSINNVGE--QLPGCLSLKRVPAHLRFSNGYRTKAASNPNLKAVEVP 60

Query: 61  RQWYNLVADLPVKPPPPLHSKTLEPLKPEDLSHLYADELIKQETSTDRYINIPDEVVDIY 120
           RQWYNLVADLPVKPPPPLH KT EP+KPEDLSHL+ DELIKQE ST+RYINIPDEVVDIY
Sbjct: 61  RQWYNLVADLPVKPPPPLHPKTYEPIKPEDLSHLFPDELIKQEASTERYINIPDEVVDIY 120

Query: 121 ELWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPSGSHKPNSAVPQVWYNVQEGVKNVVTE 180
            LWRPTPLIRAKRLEKLLDTPARIYYKYEGVSP+GSHKPNSAVPQVWYN QEGVKNVVTE
Sbjct: 121 GLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYNAQEGVKNVVTE 180

Query: 181 TGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRIMMETWGAKVHPSPSNITDVGRK 240
           TGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRIMMETWGAKVHPSPSNITDVGRK
Sbjct: 181 TGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRIMMETWGAKVHPSPSNITDVGRK 240

Query: 241 FLQVDPSSSGSLGIAISEATEIATLNVDTRYCLGSVLNHVLLHQTVIGEECLKQMEALGE 300
           FLQVDPSSSGSLGIAISEATEIATLNVDTRYCLGSVLNHVLLHQTVIGEECLKQMEALGE
Sbjct: 241 FLQVDPSSSGSLGIAISEATEIATLNVDTRYCLGSVLNHVLLHQTVIGEECLKQMEALGE 300

Query: 301 SPDVVIGCTGGGSNFAGLAFPFLREKLAGKMNPVIRAAEPAACPSLTKGVYAYDYGDTAG 360
           SPDVVIGCTGGGSNFAGLAFPFLREKLAGKMNPVIRAAEPAACPSLTKGVYAYDYGDTAG
Sbjct: 301 SPDVVIGCTGGGSNFAGLAFPFLREKLAGKMNPVIRAAEPAACPSLTKGVYAYDYGDTAG 360

Query: 361 MTPLMKMHTLGHNFIPDPIHAGGLRYHGIAPLISHVYNLGLLEAISLPQTECFRGAVQFA 420
           MTPLMKMHTLGHNFIPDPIHAGGLRYHGIAPLISHVYNLGLLEAISLPQTECFRGAVQFA
Sbjct: 361 MTPLMKMHTLGHNFIPDPIHAGGLRYHGIAPLISHVYNLGLLEAISLPQTECFRGAVQFA 420

Query: 421 RSEGLIPAPEPTHAIAATIREALRCRDTGESKVILTAMSGHGHFDLPVYEKYLQGGIRDL 480
           RSEGLIPAPEPTHAIAATIREALRCRDTGESKVILTAMSGHGHFDLPVYEKYLQGGIRDL
Sbjct: 421 RSEGLIPAPEPTHAIAATIREALRCRDTGESKVILTAMSGHGHFDLPVYEKYLQGGIRDL 480

Query: 481 SFAKEKIQESLTSIPQVA 498
           SFAKEKIQESLTSIPQVA
Sbjct: 481 SFAKEKIQESLTSIPQVA 496

BLAST of CsGy5G029250 vs. NCBI nr
Match: XP_008459438.1 (PREDICTED: tryptophan synthase beta chain 2-like isoform X3 [Cucumis melo])

HSP 1 Score: 958 bits (2476), Expect = 0.0
Identity = 475/498 (95.38%), Postives = 485/498 (97.39%), Query Frame = 0

Query: 1   MAATFHAVGSLASSGTSNVGDNKQLPGRFSLKRTPRHLRFSNGYRTKAASNPNLKSVEVP 60
           M+ATFHAVGSL+SSG  N+   +QLPGR S  RTPRHLRFSNGYRTKAASN NLKSVEVP
Sbjct: 1   MSATFHAVGSLSSSGI-NI---EQLPGRLS-PRTPRHLRFSNGYRTKAASNLNLKSVEVP 60

Query: 61  RQWYNLVADLPVKPPPPLHSKTLEPLKPEDLSHLYADELIKQETSTDRYINIPDEVVDIY 120
           RQWYNLVADLPVKPPPPLHSKTLEPLKPEDLSH YADELIKQETSTDRYINIPDEVVDIY
Sbjct: 61  RQWYNLVADLPVKPPPPLHSKTLEPLKPEDLSHFYADELIKQETSTDRYINIPDEVVDIY 120

Query: 121 ELWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPSGSHKPNSAVPQVWYNVQEGVKNVVTE 180
           +LWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPSGSHKPNSAVPQVWYNVQEGVKNVVTE
Sbjct: 121 KLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPSGSHKPNSAVPQVWYNVQEGVKNVVTE 180

Query: 181 TGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRIMMETWGAKVHPSPSNITDVGRK 240
           TGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRIMMETWGAKVHPSPSNITDVGRK
Sbjct: 181 TGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRIMMETWGAKVHPSPSNITDVGRK 240

Query: 241 FLQVDPSSSGSLGIAISEATEIATLNVDTRYCLGSVLNHVLLHQTVIGEECLKQMEALGE 300
           FLQVDPSSSGSLGIAISEATE+ATLNVDTRYCLGSVLNHVLLHQTVIGEECLKQMEALGE
Sbjct: 241 FLQVDPSSSGSLGIAISEATEVATLNVDTRYCLGSVLNHVLLHQTVIGEECLKQMEALGE 300

Query: 301 SPDVVIGCTGGGSNFAGLAFPFLREKLAGKMNPVIRAAEPAACPSLTKGVYAYDYGDTAG 360
           +PDVVIGCTGGGSNFAGL FPFLREKLAGKMNPVIRAAEPAACPSLTKGVYAYDYGDTAG
Sbjct: 301 NPDVVIGCTGGGSNFAGLVFPFLREKLAGKMNPVIRAAEPAACPSLTKGVYAYDYGDTAG 360

Query: 361 MTPLMKMHTLGHNFIPDPIHAGGLRYHGIAPLISHVYNLGLLEAISLPQTECFRGAVQFA 420
           MTPLMKMHTLGHNFIPDPIHAGGLRYHGIAPLISHV NLGLLEAISLPQTECFRGAVQFA
Sbjct: 361 MTPLMKMHTLGHNFIPDPIHAGGLRYHGIAPLISHVCNLGLLEAISLPQTECFRGAVQFA 420

Query: 421 RSEGLIPAPEPTHAIAATIREALRCRDTGESKVILTAMSGHGHFDLPVYEKYLQGGIRDL 480
           RSEGLIPAPEPTHAIAATIREALRCR+TGESKVILTAMSGHGHFDLPVYEKYL+GGI DL
Sbjct: 421 RSEGLIPAPEPTHAIAATIREALRCRETGESKVILTAMSGHGHFDLPVYEKYLRGGITDL 480

Query: 481 SFAKEKIQESLTSIPQVA 498
           SFAKEKIQESLT+IPQVA
Sbjct: 481 SFAKEKIQESLTTIPQVA 493

BLAST of CsGy5G029250 vs. ExPASy TrEMBL
Match: A0A0A0KSJ6 (Tryptophan synthase OS=Cucumis sativus OX=3659 GN=Csa_5G643340 PE=3 SV=1)

HSP 1 Score: 1011 bits (2613), Expect = 0.0
Identity = 497/498 (99.80%), Postives = 497/498 (99.80%), Query Frame = 0

Query: 1   MAATFHAVGSLASSGTSNVGDNKQLPGRFSLKRTPRHLRFSNGYRTKAASNPNLKSVEVP 60
           MAATFHAVGSLASSGTSNVGDNKQLPGRFSLKRTPRHLRFSNGYRTKAASNPNLKSVEVP
Sbjct: 1   MAATFHAVGSLASSGTSNVGDNKQLPGRFSLKRTPRHLRFSNGYRTKAASNPNLKSVEVP 60

Query: 61  RQWYNLVADLPVKPPPPLHSKTLEPLKPEDLSHLYADELIKQETSTDRYINIPDEVVDIY 120
           RQWYNLVADLPVKPPPPLHSKTLEPLKPEDLSHLYADELIKQETSTDRYINIPDEVVDIY
Sbjct: 61  RQWYNLVADLPVKPPPPLHSKTLEPLKPEDLSHLYADELIKQETSTDRYINIPDEVVDIY 120

Query: 121 ELWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPSGSHKPNSAVPQVWYNVQEGVKNVVTE 180
           ELWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPSGSHKPNSAVPQVWY VQEGVKNVVTE
Sbjct: 121 ELWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPSGSHKPNSAVPQVWYIVQEGVKNVVTE 180

Query: 181 TGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRIMMETWGAKVHPSPSNITDVGRK 240
           TGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRIMMETWGAKVHPSPSNITDVGRK
Sbjct: 181 TGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRIMMETWGAKVHPSPSNITDVGRK 240

Query: 241 FLQVDPSSSGSLGIAISEATEIATLNVDTRYCLGSVLNHVLLHQTVIGEECLKQMEALGE 300
           FLQVDPSSSGSLGIAISEATEIATLNVDTRYCLGSVLNHVLLHQTVIGEECLKQMEALGE
Sbjct: 241 FLQVDPSSSGSLGIAISEATEIATLNVDTRYCLGSVLNHVLLHQTVIGEECLKQMEALGE 300

Query: 301 SPDVVIGCTGGGSNFAGLAFPFLREKLAGKMNPVIRAAEPAACPSLTKGVYAYDYGDTAG 360
           SPDVVIGCTGGGSNFAGLAFPFLREKLAGKMNPVIRAAEPAACPSLTKGVYAYDYGDTAG
Sbjct: 301 SPDVVIGCTGGGSNFAGLAFPFLREKLAGKMNPVIRAAEPAACPSLTKGVYAYDYGDTAG 360

Query: 361 MTPLMKMHTLGHNFIPDPIHAGGLRYHGIAPLISHVYNLGLLEAISLPQTECFRGAVQFA 420
           MTPLMKMHTLGHNFIPDPIHAGGLRYHGIAPLISHVYNLGLLEAISLPQTECFRGAVQFA
Sbjct: 361 MTPLMKMHTLGHNFIPDPIHAGGLRYHGIAPLISHVYNLGLLEAISLPQTECFRGAVQFA 420

Query: 421 RSEGLIPAPEPTHAIAATIREALRCRDTGESKVILTAMSGHGHFDLPVYEKYLQGGIRDL 480
           RSEGLIPAPEPTHAIAATIREALRCRDTGESKVILTAMSGHGHFDLPVYEKYLQGGIRDL
Sbjct: 421 RSEGLIPAPEPTHAIAATIREALRCRDTGESKVILTAMSGHGHFDLPVYEKYLQGGIRDL 480

Query: 481 SFAKEKIQESLTSIPQVA 498
           SFAKEKIQESLTSIPQVA
Sbjct: 481 SFAKEKIQESLTSIPQVA 498

BLAST of CsGy5G029250 vs. ExPASy TrEMBL
Match: A0A1S4E2K1 (Tryptophan synthase OS=Cucumis melo OX=3656 GN=LOC103498573 PE=3 SV=1)

HSP 1 Score: 971 bits (2509), Expect = 0.0
Identity = 478/498 (95.98%), Postives = 487/498 (97.79%), Query Frame = 0

Query: 1   MAATFHAVGSLASSGTSNVGDNKQLPGRFSLKRTPRHLRFSNGYRTKAASNPNLKSVEVP 60
           M+ATFHAVGSL+SSG SN GD +QLPGR S  RTPRHLRFSNGYRTKAASN NLKSVEVP
Sbjct: 1   MSATFHAVGSLSSSGISNGGDIEQLPGRLS-PRTPRHLRFSNGYRTKAASNLNLKSVEVP 60

Query: 61  RQWYNLVADLPVKPPPPLHSKTLEPLKPEDLSHLYADELIKQETSTDRYINIPDEVVDIY 120
           RQWYNLVADLPVKPPPPLHSKTLEPLKPEDLSH YADELIKQETSTDRYINIPDEVVDIY
Sbjct: 61  RQWYNLVADLPVKPPPPLHSKTLEPLKPEDLSHFYADELIKQETSTDRYINIPDEVVDIY 120

Query: 121 ELWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPSGSHKPNSAVPQVWYNVQEGVKNVVTE 180
           +LWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPSGSHKPNSAVPQVWYNVQEGVKNVVTE
Sbjct: 121 KLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPSGSHKPNSAVPQVWYNVQEGVKNVVTE 180

Query: 181 TGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRIMMETWGAKVHPSPSNITDVGRK 240
           TGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRIMMETWGAKVHPSPSNITDVGRK
Sbjct: 181 TGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRIMMETWGAKVHPSPSNITDVGRK 240

Query: 241 FLQVDPSSSGSLGIAISEATEIATLNVDTRYCLGSVLNHVLLHQTVIGEECLKQMEALGE 300
           FLQVDPSSSGSLGIAISEATE+ATLNVDTRYCLGSVLNHVLLHQTVIGEECLKQMEALGE
Sbjct: 241 FLQVDPSSSGSLGIAISEATEVATLNVDTRYCLGSVLNHVLLHQTVIGEECLKQMEALGE 300

Query: 301 SPDVVIGCTGGGSNFAGLAFPFLREKLAGKMNPVIRAAEPAACPSLTKGVYAYDYGDTAG 360
           +PDVVIGCTGGGSNFAGL FPFLREKLAGKMNPVIRAAEPAACPSLTKGVYAYDYGDTAG
Sbjct: 301 NPDVVIGCTGGGSNFAGLVFPFLREKLAGKMNPVIRAAEPAACPSLTKGVYAYDYGDTAG 360

Query: 361 MTPLMKMHTLGHNFIPDPIHAGGLRYHGIAPLISHVYNLGLLEAISLPQTECFRGAVQFA 420
           MTPLMKMHTLGHNFIPDPIHAGGLRYHGIAPLISHV NLGLLEAISLPQTECFRGAVQFA
Sbjct: 361 MTPLMKMHTLGHNFIPDPIHAGGLRYHGIAPLISHVCNLGLLEAISLPQTECFRGAVQFA 420

Query: 421 RSEGLIPAPEPTHAIAATIREALRCRDTGESKVILTAMSGHGHFDLPVYEKYLQGGIRDL 480
           RSEGLIPAPEPTHAIAATIREALRCR+TGESKVILTAMSGHGHFDLPVYEKYL+GGI DL
Sbjct: 421 RSEGLIPAPEPTHAIAATIREALRCRETGESKVILTAMSGHGHFDLPVYEKYLRGGITDL 480

Query: 481 SFAKEKIQESLTSIPQVA 498
           SFAKEKIQESLT+IPQVA
Sbjct: 481 SFAKEKIQESLTTIPQVA 497

BLAST of CsGy5G029250 vs. ExPASy TrEMBL
Match: A0A1S3C9P1 (Tryptophan synthase OS=Cucumis melo OX=3656 GN=LOC103498573 PE=3 SV=1)

HSP 1 Score: 958 bits (2476), Expect = 0.0
Identity = 475/498 (95.38%), Postives = 485/498 (97.39%), Query Frame = 0

Query: 1   MAATFHAVGSLASSGTSNVGDNKQLPGRFSLKRTPRHLRFSNGYRTKAASNPNLKSVEVP 60
           M+ATFHAVGSL+SSG  N+   +QLPGR S  RTPRHLRFSNGYRTKAASN NLKSVEVP
Sbjct: 1   MSATFHAVGSLSSSGI-NI---EQLPGRLS-PRTPRHLRFSNGYRTKAASNLNLKSVEVP 60

Query: 61  RQWYNLVADLPVKPPPPLHSKTLEPLKPEDLSHLYADELIKQETSTDRYINIPDEVVDIY 120
           RQWYNLVADLPVKPPPPLHSKTLEPLKPEDLSH YADELIKQETSTDRYINIPDEVVDIY
Sbjct: 61  RQWYNLVADLPVKPPPPLHSKTLEPLKPEDLSHFYADELIKQETSTDRYINIPDEVVDIY 120

Query: 121 ELWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPSGSHKPNSAVPQVWYNVQEGVKNVVTE 180
           +LWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPSGSHKPNSAVPQVWYNVQEGVKNVVTE
Sbjct: 121 KLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPSGSHKPNSAVPQVWYNVQEGVKNVVTE 180

Query: 181 TGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRIMMETWGAKVHPSPSNITDVGRK 240
           TGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRIMMETWGAKVHPSPSNITDVGRK
Sbjct: 181 TGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRIMMETWGAKVHPSPSNITDVGRK 240

Query: 241 FLQVDPSSSGSLGIAISEATEIATLNVDTRYCLGSVLNHVLLHQTVIGEECLKQMEALGE 300
           FLQVDPSSSGSLGIAISEATE+ATLNVDTRYCLGSVLNHVLLHQTVIGEECLKQMEALGE
Sbjct: 241 FLQVDPSSSGSLGIAISEATEVATLNVDTRYCLGSVLNHVLLHQTVIGEECLKQMEALGE 300

Query: 301 SPDVVIGCTGGGSNFAGLAFPFLREKLAGKMNPVIRAAEPAACPSLTKGVYAYDYGDTAG 360
           +PDVVIGCTGGGSNFAGL FPFLREKLAGKMNPVIRAAEPAACPSLTKGVYAYDYGDTAG
Sbjct: 301 NPDVVIGCTGGGSNFAGLVFPFLREKLAGKMNPVIRAAEPAACPSLTKGVYAYDYGDTAG 360

Query: 361 MTPLMKMHTLGHNFIPDPIHAGGLRYHGIAPLISHVYNLGLLEAISLPQTECFRGAVQFA 420
           MTPLMKMHTLGHNFIPDPIHAGGLRYHGIAPLISHV NLGLLEAISLPQTECFRGAVQFA
Sbjct: 361 MTPLMKMHTLGHNFIPDPIHAGGLRYHGIAPLISHVCNLGLLEAISLPQTECFRGAVQFA 420

Query: 421 RSEGLIPAPEPTHAIAATIREALRCRDTGESKVILTAMSGHGHFDLPVYEKYLQGGIRDL 480
           RSEGLIPAPEPTHAIAATIREALRCR+TGESKVILTAMSGHGHFDLPVYEKYL+GGI DL
Sbjct: 421 RSEGLIPAPEPTHAIAATIREALRCRETGESKVILTAMSGHGHFDLPVYEKYLRGGITDL 480

Query: 481 SFAKEKIQESLTSIPQVA 498
           SFAKEKIQESLT+IPQVA
Sbjct: 481 SFAKEKIQESLTTIPQVA 493

BLAST of CsGy5G029250 vs. ExPASy TrEMBL
Match: A0A1S3CAP3 (Tryptophan synthase OS=Cucumis melo OX=3656 GN=LOC103498573 PE=3 SV=1)

HSP 1 Score: 942 bits (2436), Expect = 0.0
Identity = 462/478 (96.65%), Postives = 469/478 (98.12%), Query Frame = 0

Query: 21  DNKQLPGRFSLKRTPRHLRFSNGYRTKAASNPNLKSVEVPRQWYNLVADLPVKPPPPLHS 80
           D +QLPGR S  RTPRHLRFSNGYRTKAASN NLKSVEVPRQWYNLVADLPVKPPPPLHS
Sbjct: 36  DIEQLPGRLS-PRTPRHLRFSNGYRTKAASNLNLKSVEVPRQWYNLVADLPVKPPPPLHS 95

Query: 81  KTLEPLKPEDLSHLYADELIKQETSTDRYINIPDEVVDIYELWRPTPLIRAKRLEKLLDT 140
           KTLEPLKPEDLSH YADELIKQETSTDRYINIPDEVVDIY+LWRPTPLIRAKRLEKLLDT
Sbjct: 96  KTLEPLKPEDLSHFYADELIKQETSTDRYINIPDEVVDIYKLWRPTPLIRAKRLEKLLDT 155

Query: 141 PARIYYKYEGVSPSGSHKPNSAVPQVWYNVQEGVKNVVTETGAGQWGCSLAFACSIFGIG 200
           PARIYYKYEGVSPSGSHKPNSAVPQVWYNVQEGVKNVVTETGAGQWGCSLAFACSIFGIG
Sbjct: 156 PARIYYKYEGVSPSGSHKPNSAVPQVWYNVQEGVKNVVTETGAGQWGCSLAFACSIFGIG 215

Query: 201 CEVWQVRASYDQKPYRRIMMETWGAKVHPSPSNITDVGRKFLQVDPSSSGSLGIAISEAT 260
           CEVWQVRASYDQKPYRRIMMETWGAKVHPSPSNITDVGRKFLQVDPSSSGSLGIAISEAT
Sbjct: 216 CEVWQVRASYDQKPYRRIMMETWGAKVHPSPSNITDVGRKFLQVDPSSSGSLGIAISEAT 275

Query: 261 EIATLNVDTRYCLGSVLNHVLLHQTVIGEECLKQMEALGESPDVVIGCTGGGSNFAGLAF 320
           E+ATLNVDTRYCLGSVLNHVLLHQTVIGEECLKQMEALGE+PDVVIGCTGGGSNFAGL F
Sbjct: 276 EVATLNVDTRYCLGSVLNHVLLHQTVIGEECLKQMEALGENPDVVIGCTGGGSNFAGLVF 335

Query: 321 PFLREKLAGKMNPVIRAAEPAACPSLTKGVYAYDYGDTAGMTPLMKMHTLGHNFIPDPIH 380
           PFLREKLAGKMNPVIRAAEPAACPSLTKGVYAYDYGDTAGMTPLMKMHTLGHNFIPDPIH
Sbjct: 336 PFLREKLAGKMNPVIRAAEPAACPSLTKGVYAYDYGDTAGMTPLMKMHTLGHNFIPDPIH 395

Query: 381 AGGLRYHGIAPLISHVYNLGLLEAISLPQTECFRGAVQFARSEGLIPAPEPTHAIAATIR 440
           AGGLRYHGIAPLISHV NLGLLEAISLPQTECFRGAVQFARSEGLIPAPEPTHAIAATIR
Sbjct: 396 AGGLRYHGIAPLISHVCNLGLLEAISLPQTECFRGAVQFARSEGLIPAPEPTHAIAATIR 455

Query: 441 EALRCRDTGESKVILTAMSGHGHFDLPVYEKYLQGGIRDLSFAKEKIQESLTSIPQVA 498
           EALRCR+TGESKVILTAMSGHGHFDLPVYEKYL+GGI DLSFAKEKIQESLT+IPQVA
Sbjct: 456 EALRCRETGESKVILTAMSGHGHFDLPVYEKYLRGGITDLSFAKEKIQESLTTIPQVA 512

BLAST of CsGy5G029250 vs. ExPASy TrEMBL
Match: A0A5A7T950 (Tryptophan synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G002020 PE=3 SV=1)

HSP 1 Score: 941 bits (2433), Expect = 0.0
Identity = 461/476 (96.85%), Postives = 468/476 (98.32%), Query Frame = 0

Query: 23  KQLPGRFSLKRTPRHLRFSNGYRTKAASNPNLKSVEVPRQWYNLVADLPVKPPPPLHSKT 82
           +QLPGR S  RTPRHLRFSNGYRTKAASN NLKSVEVPRQWYNLVADLPVKPPPPLHSKT
Sbjct: 26  EQLPGRLS-PRTPRHLRFSNGYRTKAASNLNLKSVEVPRQWYNLVADLPVKPPPPLHSKT 85

Query: 83  LEPLKPEDLSHLYADELIKQETSTDRYINIPDEVVDIYELWRPTPLIRAKRLEKLLDTPA 142
           LEPLKPEDLSH YADELIKQETSTDRYINIPDEVVDIY+LWRPTPLIRAKRLEKLLDTPA
Sbjct: 86  LEPLKPEDLSHFYADELIKQETSTDRYINIPDEVVDIYKLWRPTPLIRAKRLEKLLDTPA 145

Query: 143 RIYYKYEGVSPSGSHKPNSAVPQVWYNVQEGVKNVVTETGAGQWGCSLAFACSIFGIGCE 202
           RIYYKYEGVSPSGSHKPNSAVPQVWYNVQEGVKNVVTETGAGQWGCSLAFACSIFGIGCE
Sbjct: 146 RIYYKYEGVSPSGSHKPNSAVPQVWYNVQEGVKNVVTETGAGQWGCSLAFACSIFGIGCE 205

Query: 203 VWQVRASYDQKPYRRIMMETWGAKVHPSPSNITDVGRKFLQVDPSSSGSLGIAISEATEI 262
           VWQVRASYDQKPYRRIMMETWGAKVHPSPSNITDVGRKFLQVDPSSSGSLGIAISEATE+
Sbjct: 206 VWQVRASYDQKPYRRIMMETWGAKVHPSPSNITDVGRKFLQVDPSSSGSLGIAISEATEV 265

Query: 263 ATLNVDTRYCLGSVLNHVLLHQTVIGEECLKQMEALGESPDVVIGCTGGGSNFAGLAFPF 322
           ATLNVDTRYCLGSVLNHVLLHQTVIGEECLKQMEALGE+PDVVIGCTGGGSNFAGL FPF
Sbjct: 266 ATLNVDTRYCLGSVLNHVLLHQTVIGEECLKQMEALGENPDVVIGCTGGGSNFAGLVFPF 325

Query: 323 LREKLAGKMNPVIRAAEPAACPSLTKGVYAYDYGDTAGMTPLMKMHTLGHNFIPDPIHAG 382
           LREKLAGKMNPVIRAAEPAACPSLTKGVYAYDYGDTAGMTPLMKMHTLGHNFIPDPIHAG
Sbjct: 326 LREKLAGKMNPVIRAAEPAACPSLTKGVYAYDYGDTAGMTPLMKMHTLGHNFIPDPIHAG 385

Query: 383 GLRYHGIAPLISHVYNLGLLEAISLPQTECFRGAVQFARSEGLIPAPEPTHAIAATIREA 442
           GLRYHGIAPLISHV NLGLLEAISLPQTECFRGAVQFARSEGLIPAPEPTHAIAATIREA
Sbjct: 386 GLRYHGIAPLISHVCNLGLLEAISLPQTECFRGAVQFARSEGLIPAPEPTHAIAATIREA 445

Query: 443 LRCRDTGESKVILTAMSGHGHFDLPVYEKYLQGGIRDLSFAKEKIQESLTSIPQVA 498
           LRCR+TGESKVILTAMSGHGHFDLPVYEKYL+GGI DLSFAKEKIQESLT+IPQVA
Sbjct: 446 LRCRETGESKVILTAMSGHGHFDLPVYEKYLRGGITDLSFAKEKIQESLTTIPQVA 500

BLAST of CsGy5G029250 vs. TAIR 10
Match: AT5G38530.1 (tryptophan synthase beta type 2 )

HSP 1 Score: 748.8 bits (1932), Expect = 2.8e-216
Identity = 349/470 (74.26%), Postives = 414/470 (88.09%), Query Frame = 0

Query: 32  KRTPRHLRFSNG--YRTKAA--SNPNLKSVEVPRQWYNLVADLPVKPPPPLHSKTLEPLK 91
           +++ +  R SNG   R KAA  S  N   VE+P+QWYNLVADL VKPPPPLH KT EP+K
Sbjct: 36  RKSNQATRVSNGSSLRVKAALRSTHNKSVVEIPKQWYNLVADLSVKPPPPLHPKTFEPIK 95

Query: 92  PEDLSHLYADELIKQETSTDRYINIPDEVVDIYELWRPTPLIRAKRLEKLLDTPARIYYK 151
           PEDL+HL+ +ELIKQE + +R+I+IP+EV++IY+LWRPTPLIRAKRLEKLL TPARIY+K
Sbjct: 96  PEDLAHLFPNELIKQEATQERFIDIPEEVLEIYKLWRPTPLIRAKRLEKLLQTPARIYFK 155

Query: 152 YEGVSPSGSHKPNSAVPQVWYNVQEGVKNVVTETGAGQWGCSLAFACSIFGIGCEVWQVR 211
           YEG SP+GSHKPN+AVPQ +YN +EGVKNVVTETGAGQWG SLAFA S+FG+ CEVWQV 
Sbjct: 156 YEGGSPAGSHKPNTAVPQAYYNAKEGVKNVVTETGAGQWGSSLAFASSLFGLDCEVWQVA 215

Query: 212 ASYDQKPYRRIMMETWGAKVHPSPSNITDVGRKFLQVDPSSSGSLGIAISEATEIATLNV 271
            SY  KPYRR+MM+TWGAKVHPSPS++T+ GR+ L+ DPSS GSLGIAISEA E+A  N 
Sbjct: 216 NSYHTKPYRRLMMQTWGAKVHPSPSDLTEAGRRILESDPSSPGSLGIAISEAVEVAARNE 275

Query: 272 DTRYCLGSVLNHVLLHQTVIGEECLKQMEALGESPDVVIGCTGGGSNFAGLAFPFLREKL 331
           DT+YCLGSVLNHVLLHQT+IGEEC++QME  GE+PD++IGCTGGGSNFAGL+FPF+REKL
Sbjct: 276 DTKYCLGSVLNHVLLHQTIIGEECIQQMENFGETPDLIIGCTGGGSNFAGLSFPFIREKL 335

Query: 332 AGKMNPVIRAAEPAACPSLTKGVYAYDYGDTAGMTPLMKMHTLGHNFIPDPIHAGGLRYH 391
            GK+NPVIRA EP+ACPSLTKGVYAYD+GDTAG+TPLMKMHTLGH+FIPDPIHAGGLRYH
Sbjct: 336 KGKINPVIRAVEPSACPSLTKGVYAYDFGDTAGLTPLMKMHTLGHDFIPDPIHAGGLRYH 395

Query: 392 GIAPLISHVYNLGLLEAISLPQTECFRGAVQFARSEGLIPAPEPTHAIAATIREALRCRD 451
           G+APLISHVY  G +EAIS+PQ ECF+GA+QFAR+EG+IPAPEPTHAIAATIREALRC++
Sbjct: 396 GMAPLISHVYEQGFMEAISIPQIECFQGAIQFARTEGIIPAPEPTHAIAATIREALRCKE 455

Query: 452 TGESKVILTAMSGHGHFDLPVYEKYLQGGIRDLSFAKEKIQESLTSIPQV 498
           TGE+KVIL AM GHGHFDL  Y+KYL+G + DLSF++EKI+ESL+ +P V
Sbjct: 456 TGEAKVILMAMCGHGHFDLTSYDKYLKGELVDLSFSEEKIRESLSKVPHV 505

BLAST of CsGy5G029250 vs. TAIR 10
Match: AT4G27070.1 (tryptophan synthase beta-subunit 2 )

HSP 1 Score: 126.3 bits (316), Expect = 6.8e-29
Identity = 120/405 (29.63%), Postives = 176/405 (43.46%), Query Frame = 0

Query: 88  PEDLSHLYAD-ELIKQETSTDRYINIPDEVVDIYE--LWRPTPLIRAKRL----EKLLDT 147
           PE L H  ++ E      +TD   +   E+ +I +  + R +PL  A+RL     +    
Sbjct: 95  PETLMHALSELETAFYSLATDE--DFQRELAEILKDYVGRESPLYFAERLTEHYRRENGE 154

Query: 148 PARIYYKYEGVSPSGSHKPNSAVPQVWYNVQEGVKNVVTETGAGQWGCSLAFACSIFGIG 207
              IY K E ++ +G+HK N+AV Q     + G K ++ ETGAGQ G + A  C+ FG+ 
Sbjct: 155 GPLIYLKREDLNHTGAHKINNAVAQALLAKRLGKKRIIAETGAGQHGVATATVCARFGLQ 214

Query: 208 CEVWQVRASYDQKPYRRIMMETWGAKVHPSPSNITDVGRKFLQVDPSSSGSLGIAISEAT 267
           C ++      +++      M   GA+V                   S + +L  A SEA 
Sbjct: 215 CIIYMGAQDMERQALNVFRMRLLGAEVRGV---------------HSGTATLKDATSEAI 274

Query: 268 EIATLNVD-TRYCLGSVLN------HVLLHQTVIGEECLKQ-MEALGESPDVVIGCTGGG 327
                NV+ T Y LGSV         V     VIG+E  KQ ME  G  PDV++ C GGG
Sbjct: 275 RDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQAMEKWGGKPDVLVACVGGG 334

Query: 328 SNFAGLAFPFLRE---KLAGKMNPVIRAAEPAACPSLTKGVYAYDYGDTAGMTPLMKMHT 387
           SN  GL   F+ +   ++ G               +LTKG    D G   G    +    
Sbjct: 335 SNAMGLFHEFVDDTEVRMIGVEAAGFGLDSGKHAATLTKG----DVGVLHGAMSYLLQDD 394

Query: 388 LGHNFIPDPIHAGGLRYHGIAPLISHVYNLGLLEAISLPQTECFRGAVQFARSEGLIPAP 447
            G    P  I A GL Y G+ P  S + ++G  E  S+   E      + +R EG+IPA 
Sbjct: 395 DGQIIEPHSISA-GLDYPGVGPEHSFLKDVGRAEYFSVTDEEALEAFKRVSRLEGIIPAL 454

Query: 448 EPTHAIAATIREALRCRDTGESKVILTAMSGHGHFDLPVYEKYLQ 475
           E +HA+A   +    C    +   ++   SG G  D+    KYL+
Sbjct: 455 ETSHALAHLEK---LCPTLPDGARVVLNFSGRGDKDVQTAIKYLE 474

BLAST of CsGy5G029250 vs. TAIR 10
Match: AT5G54810.1 (tryptophan synthase beta-subunit 1 )

HSP 1 Score: 124.8 bits (312), Expect = 2.0e-28
Identity = 104/341 (30.50%), Postives = 148/341 (43.40%), Query Frame = 0

Query: 144 IYYKYEGVSPSGSHKPNSAVPQVWYNVQEGVKNVVTETGAGQWGCSLAFACSIFGIGCEV 203
           IY K E ++ +G+HK N+AV Q     + G K ++ ETGAGQ G + A  C+ FG+ C +
Sbjct: 151 IYLKREDLNHTGAHKINNAVAQALLAKRLGKKRIIAETGAGQHGVATATVCARFGLECII 210

Query: 204 WQVRASYDQKPYRRIMMETWGAKVHPSPSNITDVGRKFLQVDPSSSGSLGIAISEATEIA 263
           +      +++      M   GA+V                   S + +L  A SEA    
Sbjct: 211 YMGAQDMERQALNVFRMRLLGAEVRGV---------------HSGTATLKDATSEAIRDW 270

Query: 264 TLNVD-TRYCLGSVLN------HVLLHQTVIGEECLKQ-MEALGESPDVVIGCTGGGSNF 323
             NV+ T Y LGSV         V     VIG+E  KQ +E  G  PDV++ C GGGSN 
Sbjct: 271 VTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKWGGKPDVLVACVGGGSNA 330

Query: 324 AGLAFPFLRE---KLAGKMNPVIRAAEPAACPSLTKGVYAYDYGDTAGMTPLMKMHTLGH 383
            GL   F+ +   ++ G               +LTKG    D G   G    +     G 
Sbjct: 331 MGLFHEFVNDTEVRMIGVEAAGFGLDSGKHAATLTKG----DVGVLHGAMSYLLQDDDGQ 390

Query: 384 NFIPDPIHAGGLRYHGIAPLISHVYNLGLLEAISLPQTECFRGAVQFARSEGLIPAPEPT 443
              P  I A GL Y G+ P  S   ++G  E  S+   E      + +R EG+IPA E +
Sbjct: 391 IIEPHSISA-GLDYPGVGPEHSFFKDMGRAEYYSITDEEALEAFKRVSRLEGIIPALETS 450

Query: 444 HAIAATIREALRCRDTGESKVILTAMSGHGHFDLPVYEKYL 474
           HA+A   +    C    +   ++   SG G  D+    KYL
Sbjct: 451 HALAYLEK---LCPTLSDGTRVVLNFSGRGDKDVQTVAKYL 468

BLAST of CsGy5G029250 vs. TAIR 10
Match: AT5G28237.1 (Pyridoxal-5'-phosphate-dependent enzyme family protein )

HSP 1 Score: 116.7 bits (291), Expect = 5.4e-26
Identity = 109/362 (30.11%), Postives = 160/362 (44.20%), Query Frame = 0

Query: 124 RPTPLIRAKRLEK-------LLDTPARIYYKYEGVSPSGSHKPNSAVPQVWYNVQEGVKN 183
           R TPL  A+RL +        ++    IY K E +S  GSHK N+A+ Q   + + G   
Sbjct: 118 RETPLYFAERLTEHYKNIVPTIEGGPEIYLKREDLSHCGSHKINNALAQAMISRRLGCSR 177

Query: 184 VVTETGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRIMMETWGAKVHPSPSNITD 243
           VV  TGAGQ G + A AC+   + C V+   A  +++ +  + M+  GA+V         
Sbjct: 178 VVAATGAGQHGVATAAACAKLSLECTVFMGAADIEKQSFNVLSMKLLGAQV--------- 237

Query: 244 VGRKFLQVDPSSSGSLGIAISEATEIATLNVDTRYCL-GSVLNH------VLLHQTVIGE 303
                     S  G+   A SEA      N+ T Y L G+V+        V   Q+VIG+
Sbjct: 238 ---------ISVEGTFKDASSEAIRNWVENLYTTYYLSGTVVGPHPCPIIVREFQSVIGK 297

Query: 304 ECLKQMEAL-GESPDVVIGCTGGGSNFAGLAFPFLRE---KLAGKMNPVIRAAEPAACPS 363
           E  +Q + L G  PDV++ C G GSN  GL   F+ +   +L G     + AA       
Sbjct: 298 ETRRQAKQLWGGKPDVLVACVGSGSNALGLFHEFVGDEDVRLVG-----VEAAGLGLDSG 357

Query: 364 LTKGVYAY-DYGDTAGMTPLMKMHTLGHNFIPDPIHAGGLRYHGIAPLISHVYNLGLLEA 423
                 A+ D G   G    +     G    P  +   GL Y G+ P IS +   G  E 
Sbjct: 358 KHSATLAFGDVGVYHGSMSYLLQDDQGQILKPHSVGV-GLEYPGVGPEISFMKETGRAEF 417

Query: 424 ISLPQTECFRGAVQFARSEGLIPAPEPTHAIAATIREALRCRDTGESKVILTAMSGHGHF 467
            +    E  +  ++ +R EG+IPA E +HA+A   +     RD   +KV++   SG G  
Sbjct: 418 YTATDEEAIQACMRLSRLEGIIPALEASHALAFLDKLVPTLRD--GAKVVVNC-SGRGDK 452

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O674097.5e-15059.21Tryptophan synthase beta chain 2 OS=Aquifex aeolicus (strain VF5) OX=224324 GN=t... [more]
Q8Q0011.6e-14758.61Tryptophan synthase beta chain 2 OS=Methanosarcina mazei (strain ATCC BAA-159 / ... [more]
O290285.0e-14657.75Tryptophan synthase beta chain 2 OS=Archaeoglobus fulgidus (strain ATCC 49558 / ... [more]
Q8TL445.6e-14556.94Tryptophan synthase beta chain 2 OS=Methanosarcina acetivorans (strain ATCC 3539... [more]
Q9WZ097.8e-13957.79Tryptophan synthase beta chain 2 OS=Thermotoga maritima (strain ATCC 43589 / DSM... [more]
Match NameE-valueIdentityDescription
XP_011656025.20.0100.00uncharacterized protein LOC101219882 isoform X1 [Cucumis sativus] >XP_011656026.... [more]
XP_031742108.10.099.00uncharacterized protein LOC101219882 isoform X2 [Cucumis sativus] >XP_031742109.... [more]
XP_008459435.10.095.98PREDICTED: tryptophan synthase beta chain 2-like isoform X2 [Cucumis melo] >XP_0... [more]
KAE8648956.10.095.18hypothetical protein Csa_007904 [Cucumis sativus][more]
XP_008459438.10.095.38PREDICTED: tryptophan synthase beta chain 2-like isoform X3 [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A0A0KSJ60.099.80Tryptophan synthase OS=Cucumis sativus OX=3659 GN=Csa_5G643340 PE=3 SV=1[more]
A0A1S4E2K10.095.98Tryptophan synthase OS=Cucumis melo OX=3656 GN=LOC103498573 PE=3 SV=1[more]
A0A1S3C9P10.095.38Tryptophan synthase OS=Cucumis melo OX=3656 GN=LOC103498573 PE=3 SV=1[more]
A0A1S3CAP30.096.65Tryptophan synthase OS=Cucumis melo OX=3656 GN=LOC103498573 PE=3 SV=1[more]
A0A5A7T9500.096.85Tryptophan synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G... [more]
Match NameE-valueIdentityDescription
AT5G38530.12.8e-21674.26tryptophan synthase beta type 2 [more]
AT4G27070.16.8e-2929.63tryptophan synthase beta-subunit 2 [more]
AT5G54810.12.0e-2830.50tryptophan synthase beta-subunit 1 [more]
AT5G28237.15.4e-2630.11Pyridoxal-5'-phosphate-dependent enzyme family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006316Tryptophan synthase, beta chain-likePIRSFPIRSF500824TrpB_prokcoord: 47..482
e-value: 2.9E-194
score: 643.5
IPR006316Tryptophan synthase, beta chain-likeTIGRFAMTIGR01415TIGR01415coord: 59..475
e-value: 1.8E-187
score: 621.1
IPR006316Tryptophan synthase, beta chain-likePANTHERPTHR48077:SF6TRYPTOPHAN SYNTHASE BETA CHAINcoord: 52..497
IPR023026Tryptophan synthase beta chain/beta chain-likePIRSFPIRSF001413Trp_syn_betacoord: 55..479
e-value: 4.6E-114
score: 379.7
IPR023026Tryptophan synthase beta chain/beta chain-likeHAMAPMF_00133Trp_synth_betacoord: 67..473
score: 38.421684
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeGENE3D3.40.50.1100coord: 75..285
e-value: 3.5E-146
score: 489.1
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeGENE3D3.40.50.1100coord: 124..466
e-value: 3.5E-146
score: 489.1
IPR036052Tryptophan synthase beta subunit-like PLP-dependent enzymeSUPERFAMILY53686Tryptophan synthase beta subunit-like PLP-dependent enzymescoord: 76..472
IPR001926Pyridoxal-phosphate dependent enzymePFAMPF00291PALPcoord: 122..460
e-value: 6.0E-40
score: 137.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 9..24
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..32
NoneNo IPR availablePANTHERPTHR48077TRYPTOPHAN SYNTHASE-RELATEDcoord: 52..497
IPR006653Tryptophan synthase, beta chain, conserved sitePROSITEPS00168TRP_SYNTHASE_BETAcoord: 151..165
IPR006654Tryptophan synthase, beta chainCDDcd06446Trp-synth_Bcoord: 98..469
e-value: 1.76866E-172
score: 488.199

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy5G029250.2CsGy5G029250.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000162 tryptophan biosynthetic process
biological_process GO:0006568 tryptophan metabolic process
molecular_function GO:0052684 L-serine hydro-lyase (adding indole, L-tryptophan-forming) activity
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0004834 tryptophan synthase activity