CsGy5G029240 (gene) Cucumber (Gy14) v2.1

BLAST of CsGy5G029240 vs. ExPASy Swiss-Prot
Match: O67409 (Tryptophan synthase beta chain 2 OS=Aquifex aeolicus (strain VF5) OX=224324 GN=trpB2 PE=3 SV=1)

HSP 1 Score: 549.7 bits (1415), Expect = 3.5e-155
Identity = 259/425 (60.94%), Postives = 325/425 (76.47%), Query Frame = 0

Query: 56  EVPRQWYNLVADLPVKPPPPLHPKTYEPIKPEDLSHLFPDELIKQEASTERYINIPDEVV 115
           E+P++W N++  LP    PPL P+T EP+KPE L  +FP+ L++QE S + +I+IP+EV+
Sbjct: 10  EIPKKWLNILPLLPEPLEPPLDPETMEPVKPEKLLAIFPEPLVEQEVSDKEWIDIPEEVL 69

Query: 116 DIYGLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYNAQEGVKNV 175
           DIY LWRPTPL RAK LE+ L TPA+I+YK E VSP GSHKPN+AV Q +YN   GVK +
Sbjct: 70  DIYSLWRPTPLHRAKNLEEFLGTPAKIFYKNESVSPPGSHKPNTAVAQAYYNKISGVKRL 129

Query: 176 VTETGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSDITDA 235
            TETGAGQWG +L+FA   F + C V+ VR SY+QKPYRR++METW  +V PSPS  T+A
Sbjct: 130 TTETGAGQWGSALSFATQFFDLQCRVYMVRVSYNQKPYRRILMETWKGEVIPSPSPYTNA 189

Query: 236 GRKFLQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQLEA 295
           GRK+ + +P  PGSLGIAISEA+E AA   DTKY LGSVLNHVLLHQTVIG E  KQ+E 
Sbjct: 190 GRKYYEENPEHPGSLGIAISEAIEEAASREDTKYSLGSVLNHVLLHQTVIGLEAKKQMEE 249

Query: 296 LGETPDLIIGCTGGGSNFAGLSFPFLREKLAGNINP---VIRAVEPAACPSLTKGVYAYD 355
            G  PD+IIG  GGGSNFAGLSFPFL + L G+       + AVEP ACP+LTKG Y YD
Sbjct: 250 AGYYPDVIIGAVGGGSNFAGLSFPFLADVLRGDKRKEDLKVLAVEPEACPTLTKGEYKYD 309

Query: 356 YGDTAGMTPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQ 415
           +GD+ G+TPL+KM+TLGH+F+P PIHAGGLRYHG APL+  +YNLG ++A++  QTE F+
Sbjct: 310 FGDSVGLTPLIKMYTLGHDFVPSPIHAGGLRYHGDAPLVCKLYNLGYIDAVAYKQTEVFE 369

Query: 416 GAIQFARSEGLIPAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQ 475
            A+ FAR+EG++PAPE  HAI A I EAL+C+ETGE KVIL  + GHG+FDL AY+KYL 
Sbjct: 370 AAVTFARTEGIVPAPESAHAIKAAIDEALKCKETGEEKVILFNLSGHGYFDLSAYDKYLH 429

Query: 476 GGISD 478
           G ++D
Sbjct: 430 GELTD 434

BLAST of CsGy5G029240 vs. ExPASy Swiss-Prot
Match: O29028 (Tryptophan synthase beta chain 2 OS=Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) OX=224325 GN=trpB2 PE=3 SV=1)

HSP 1 Score: 541.6 bits (1394), Expect = 9.4e-153
Identity = 255/422 (60.43%), Postives = 322/422 (76.30%), Query Frame = 0

Query: 56  EVPRQWYNLVADLPVKPPPPLHPKTYEPIKPEDLSHLFPDELIKQEASTERYINIPDEVV 115
           E+P++WYN++ DLP   PPPLHP T EP+KPEDL  +FP  LI+QE S ER+I IP++V 
Sbjct: 15  EMPKEWYNVLPDLPEPLPPPLHPATQEPVKPEDLEPIFPKGLIQQEMSGERWIRIPEDVR 74

Query: 116 DIYGLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYNAQEGVKNV 175
           +IY +WRPTPL+RA+RLEK L TPARIY+KYEG SP GSHKPN+AV Q +YNA+EGV+ +
Sbjct: 75  EIYRIWRPTPLVRAERLEKALKTPARIYFKYEGASPPGSHKPNTAVAQAYYNAKEGVERL 134

Query: 176 VTETGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSDITDA 235
            TETGAGQWG +L FA  +F + C V+ V+ S+ QKPYRR+MMETWG +V PSPSD T+ 
Sbjct: 135 TTETGAGQWGSALCFATKLFEMACTVYMVKVSFMQKPYRRVMMETWGGEVIPSPSDRTEV 194

Query: 236 GRKFLQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQLEA 295
           GRK L  +P +PGSLGIAISEA+E AA N +TKY LGSVLNHVLLHQTVIG E   QLE 
Sbjct: 195 GRKILAENPDTPGSLGIAISEAIEDAAKNENTKYSLGSVLNHVLLHQTVIGLETKAQLEK 254

Query: 296 LGETPDLIIGCTGGGSNFAGLSFPFLREKLAGNINPVIRAVEPAACPSLTKGVYAYDYGD 355
           + E PD++IGC GGGSNFAGL++PF+ +   G++   I AVEPAACP+LT G Y YD+GD
Sbjct: 255 VDEKPDVLIGCVGGGSNFAGLTYPFVNDAKNGDLE--IIAVEPAACPTLTAGEYKYDFGD 314

Query: 356 TAGMTPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAI 415
            AG+TPL+KM+TLGH+FIP PIHAGGLRYHG AP +  +   G+++A ++ Q   F+  +
Sbjct: 315 VAGLTPLLKMYTLGHDFIPPPIHAGGLRYHGDAPTLCMLVKHGVIKARAVKQLPTFEAGL 374

Query: 416 QFARSEGLIPAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGI 475
            FAR+EG+IPAPE  HA+ A I EA++ RE  E KVI+    GHG  DL AY+ YL G +
Sbjct: 375 LFARTEGIIPAPETNHAVRAAIDEAIKARENNEEKVIVFGFSGHGLLDLQAYDDYLAGRL 434

Query: 476 SD 478
           +D
Sbjct: 435 AD 434

BLAST of CsGy5G029240 vs. ExPASy Swiss-Prot
Match: Q8Q001 (Tryptophan synthase beta chain 2 OS=Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88) OX=192952 GN=trpB2 PE=3 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 5.7e-150
Identity = 247/418 (59.09%), Postives = 318/418 (76.08%), Query Frame = 0

Query: 56  EVPRQWYNLVADLPVKPPPPLHPKTYEPIKPEDLSHLFPDELIKQEASTERYINIPDEVV 115
           E+P++WYN+++DLP    PPL P+T++PI PE L  +F  ELI+QE S++RYI+IP E++
Sbjct: 22  EMPKRWYNVLSDLPSPIDPPLDPRTWQPINPEALEPIFAKELIRQEMSSDRYIDIPAEIL 81

Query: 116 DIYGLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYNAQEGVKNV 175
           D+Y LWRP+PL RA +LEK L TPA+IYYKYEGVSPAGSHK N+++ Q +YN +EG + +
Sbjct: 82  DVYRLWRPSPLFRAHQLEKDLKTPAKIYYKYEGVSPAGSHKTNTSIAQAYYNMKEGTERL 141

Query: 176 VTETGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSDITDA 235
            TETGAGQWG +L+ AC+ F + C+V+ VR+SY QKPYR+ +M  WG  V PSPS  T+ 
Sbjct: 142 TTETGAGQWGSALSLACNYFDLECKVYMVRSSYYQKPYRKSLMTLWGGNVVPSPSPDTEF 201

Query: 236 GRKFLQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQLEA 295
           GRK L+  P +PGSLGIAISEAVE A  + +TKY LGSVLNHV+LHQTVIG EC KQLE 
Sbjct: 202 GRKILKEQPDTPGSLGIAISEAVEDAIAHDNTKYTLGSVLNHVVLHQTVIGAECKKQLEQ 261

Query: 296 LGETPDLIIGCTGGGSNFAGLSFPFLREKLAGNINPVIRAVEPAACPSLTKGVYAYDYGD 355
           + E PD++IGC GGGSN  G+   F++++L G  N  + AVEP+ACPSLTKG Y YD+GD
Sbjct: 262 VEEYPDVVIGCCGGGSNLGGIGLEFIKDRLEGKHNARVVAVEPSACPSLTKGEYRYDFGD 321

Query: 356 TAGMTPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAI 415
           TA MTPL+KM+TLGH  IP  IHAGGLRYHG +P+IS +   GL+EA+S  Q E F  A+
Sbjct: 322 TAEMTPLLKMYTLGHKHIPPAIHAGGLRYHGDSPIISKLCAEGLMEAVSYGQKEVFDAAV 381

Query: 416 QFARSEGLIPAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQG 474
           QFAR+EG++PAPE +HAI   I EAL  ++ GE KVIL  + GHGHFD+ +Y+KY  G
Sbjct: 382 QFARTEGIVPAPESSHAIRCAIDEALEAKQKGEEKVILFNLSGHGHFDMASYDKYFSG 439

BLAST of CsGy5G029240 vs. ExPASy Swiss-Prot
Match: Q8TL44 (Tryptophan synthase beta chain 2 OS=Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) OX=188937 GN=trpB2 PE=3 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 6.3e-149
Identity = 242/418 (57.89%), Postives = 319/418 (76.32%), Query Frame = 0

Query: 56  EVPRQWYNLVADLPVKPPPPLHPKTYEPIKPEDLSHLFPDELIKQEASTERYINIPDEVV 115
           E+P++WYN++ADLP    PPL P+T++PI P+ L  +FP  LI QE S++RYI+IP+EV+
Sbjct: 22  EMPKKWYNVLADLPSPIDPPLDPRTWQPISPDALEPIFPKALIMQEMSSDRYIDIPEEVL 81

Query: 116 DIYGLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYNAQEGVKNV 175
           D+Y LWRP+PL RA +LEK+L +PA+IYYKYEGVSPAGSHK N+++ Q +YN +EG + +
Sbjct: 82  DVYRLWRPSPLFRAHQLEKVLKSPAKIYYKYEGVSPAGSHKTNTSIAQAYYNMKEGTERL 141

Query: 176 VTETGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSDITDA 235
            TETGAGQWG +L+ AC+ F + C+V+ VR+S+ QKPYR+ ++  WG  V PSPS  T+ 
Sbjct: 142 TTETGAGQWGSALSLACNYFDLECKVYMVRSSFYQKPYRKSLITLWGGNVVPSPSPDTEF 201

Query: 236 GRKFLQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQLEA 295
           GRK LQ  P +PGSLGIAISEAVE A  + +TKY LGSVLNHV+LHQTVIG EC +QL  
Sbjct: 202 GRKILQEQPDTPGSLGIAISEAVEDAIAHENTKYSLGSVLNHVVLHQTVIGAECKQQLAQ 261

Query: 296 LGETPDLIIGCTGGGSNFAGLSFPFLREKLAGNINPVIRAVEPAACPSLTKGVYAYDYGD 355
           + E PD++IGC GGGSN  G+   F++++L G  +  + AVEP+ACPSLTKG Y YD+GD
Sbjct: 262 VEEYPDVVIGCCGGGSNLGGIGLEFIKDRLEGKHSARVVAVEPSACPSLTKGEYRYDFGD 321

Query: 356 TAGMTPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAI 415
           TA MTPL+KM+TLGH  +P  IHAGGLRYHG +P+IS + + GL+EA+S  Q E F  A+
Sbjct: 322 TAEMTPLLKMYTLGHKHVPPAIHAGGLRYHGDSPIISKLCSEGLMEAVSYDQQEVFDAAV 381

Query: 416 QFARSEGLIPAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQG 474
           QFAR+EG++PAPE +HAI   I EAL  ++TGE K IL  + GHGHFD+ +Y+KY  G
Sbjct: 382 QFARTEGIVPAPESSHAIRCAIDEALAAKQTGEEKTILFNLSGHGHFDMSSYDKYFSG 439

BLAST of CsGy5G029240 vs. ExPASy Swiss-Prot
Match: Q9WZ09 (Tryptophan synthase beta chain 2 OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=trpB2 PE=3 SV=1)

HSP 1 Score: 511.5 bits (1316), Expect = 1.0e-143
Identity = 251/417 (60.19%), Postives = 312/417 (74.82%), Query Frame = 0

Query: 51  NLKAVEVPRQWYNLVADLPVKPPPPLHPKTYEPIKPEDLSHLFPDELIKQEASTERYINI 110
           NLK  E+P+ WYN++ADLP K  PPL P+T +PI PE LS +FP  LI+QE S ER+I I
Sbjct: 6   NLKPEEIPKHWYNVLADLPFKLDPPLDPETKQPISPEKLSVIFPMSLIEQEVSEERFIEI 65

Query: 111 PDEVVDIYGLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYNAQE 170
           P+ V+  Y ++RPTPLIRA  LE+ L TPARIYYKYEGVSP GSHKPN+A+ Q +YN  E
Sbjct: 66  PEPVLKEYAVYRPTPLIRATFLEEYLQTPARIYYKYEGVSPTGSHKPNTAIAQAYYNKIE 125

Query: 171 GVKNVVTETGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPS 230
           GVK +VTETGAGQWG +L++A + FG+  +V+ V+ SY QKP R+ MM  +G KV PSPS
Sbjct: 126 GVKRLVTETGAGQWGSALSYAGAKFGLEVKVFMVKVSYQQKPMRKYMMNLFGGKVTPSPS 185

Query: 231 DITDAGRKFLQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECL 290
           + T+ GRK L  DP +PGSLGIAISEA+EVA  + +TKY LGSVLNHVLLHQTVIG E  
Sbjct: 186 EETNFGRKILSEDPDNPGSLGIAISEALEVAVSDPNTKYSLGSVLNHVLLHQTVIGLEIK 245

Query: 291 KQLEALGETPDLIIGCTGGGSNFAGLSFPFLREKLAGNINPVIRAVEPAACPSLTKGVYA 350
           KQLE +GE PD+++GC GGGSNF G   PF+ +KL+G     + A EPAACPSLTKG Y 
Sbjct: 246 KQLELIGEKPDILLGCHGGGSNFGGTILPFVPDKLSGRDIRFV-ACEPAACPSLTKGNYD 305

Query: 351 YDYGDTAGMTPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTEC 410
           YD+GDTAG+TPL+KM+TLG +FIP  IHAGGLRYHG AP+I+ +   GL+EA +  Q E 
Sbjct: 306 YDFGDTAGLTPLLKMYTLGKDFIPPKIHAGGLRYHGSAPIIARLVKEGLVEAQAFDQDET 365

Query: 411 FQGAIQFARSEGLIPAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAY 468
           F+ A  FA+ EG+IPAPE  HAIA  IREA + +E G+ +VI+  + GHG  DL AY
Sbjct: 366 FEAAKIFAKLEGIIPAPESAHAIAGAIREAKKAKEEGKERVIVFTLSGHGLLDLTAY 421

BLAST of CsGy5G029240 vs. NCBI nr
Match: XP_031741291.1 (uncharacterized protein LOC101219640 [Cucumis sativus])

HSP 1 Score: 1018 bits (2633), Expect = 0.0
Identity = 496/496 (100.00%), Postives = 496/496 (100.00%), Query Frame = 0

Query: 1   MAATFLTVGSLTSSSINNVGEQLPGCLSLKRVPAHLRFSNGYRTKAASNPNLKAVEVPRQ 60
           MAATFLTVGSLTSSSINNVGEQLPGCLSLKRVPAHLRFSNGYRTKAASNPNLKAVEVPRQ
Sbjct: 1   MAATFLTVGSLTSSSINNVGEQLPGCLSLKRVPAHLRFSNGYRTKAASNPNLKAVEVPRQ 60

Query: 61  WYNLVADLPVKPPPPLHPKTYEPIKPEDLSHLFPDELIKQEASTERYINIPDEVVDIYGL 120
           WYNLVADLPVKPPPPLHPKTYEPIKPEDLSHLFPDELIKQEASTERYINIPDEVVDIYGL
Sbjct: 61  WYNLVADLPVKPPPPLHPKTYEPIKPEDLSHLFPDELIKQEASTERYINIPDEVVDIYGL 120

Query: 121 WRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYNAQEGVKNVVTETG 180
           WRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYNAQEGVKNVVTETG
Sbjct: 121 WRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYNAQEGVKNVVTETG 180

Query: 181 AGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSDITDAGRKFL 240
           AGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSDITDAGRKFL
Sbjct: 181 AGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSDITDAGRKFL 240

Query: 241 QVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQLEALGETP 300
           QVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQLEALGETP
Sbjct: 241 QVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQLEALGETP 300

Query: 301 DLIIGCTGGGSNFAGLSFPFLREKLAGNINPVIRAVEPAACPSLTKGVYAYDYGDTAGMT 360
           DLIIGCTGGGSNFAGLSFPFLREKLAGNINPVIRAVEPAACPSLTKGVYAYDYGDTAGMT
Sbjct: 301 DLIIGCTGGGSNFAGLSFPFLREKLAGNINPVIRAVEPAACPSLTKGVYAYDYGDTAGMT 360

Query: 361 PLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFARS 420
           PLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFARS
Sbjct: 361 PLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFARS 420

Query: 421 EGLIPAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGISDLSF 480
           EGLIPAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGISDLSF
Sbjct: 421 EGLIPAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGISDLSF 480

Query: 481 ANEKIQESLTTIPQVA 496
           ANEKIQESLTTIPQVA
Sbjct: 481 ANEKIQESLTTIPQVA 496

BLAST of CsGy5G029240 vs. NCBI nr
Match: XP_008459440.1 (PREDICTED: tryptophan synthase beta chain 2-like [Cucumis melo])

HSP 1 Score: 1008 bits (2605), Expect = 0.0
Identity = 486/496 (97.98%), Postives = 495/496 (99.80%), Query Frame = 0

Query: 1   MAATFLTVGSLTSSSINNVGEQLPGCLSLKRVPAHLRFSNGYRTKAASNPNLKAVEVPRQ 60
           MAATFLTVGSLTSSSINNVGEQLPGCLSLKRVP HLRFSNGYRTK+ASNPNLKAVE+PRQ
Sbjct: 1   MAATFLTVGSLTSSSINNVGEQLPGCLSLKRVPTHLRFSNGYRTKSASNPNLKAVEIPRQ 60

Query: 61  WYNLVADLPVKPPPPLHPKTYEPIKPEDLSHLFPDELIKQEASTERYINIPDEVVDIYGL 120
           WYNLVADLPVKPPPPLHPKTYEPIKPEDLSHLFPDELIKQEASTERYINIPDEVVDIYGL
Sbjct: 61  WYNLVADLPVKPPPPLHPKTYEPIKPEDLSHLFPDELIKQEASTERYINIPDEVVDIYGL 120

Query: 121 WRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYNAQEGVKNVVTETG 180
           WRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYNAQEGVKNVVTETG
Sbjct: 121 WRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYNAQEGVKNVVTETG 180

Query: 181 AGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSDITDAGRKFL 240
           AGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSD+TDAGRKFL
Sbjct: 181 AGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSDVTDAGRKFL 240

Query: 241 QVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQLEALGETP 300
           QVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQLEALGE+P
Sbjct: 241 QVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQLEALGESP 300

Query: 301 DLIIGCTGGGSNFAGLSFPFLREKLAGNINPVIRAVEPAACPSLTKGVYAYDYGDTAGMT 360
           DLIIGCTGGGSNFAGLSFPFLREKLAGNINPVIRAVEPAACPSLTKGVYAYDYGDTAGMT
Sbjct: 301 DLIIGCTGGGSNFAGLSFPFLREKLAGNINPVIRAVEPAACPSLTKGVYAYDYGDTAGMT 360

Query: 361 PLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFARS 420
           PLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEA+SLPQTECFQGAI+FARS
Sbjct: 361 PLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAVSLPQTECFQGAIKFARS 420

Query: 421 EGLIPAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGISDLSF 480
           EGLIPAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGI+DLSF
Sbjct: 421 EGLIPAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGITDLSF 480

Query: 481 ANEKIQESLTTIPQVA 496
           ANEK++ESLTTIPQVA
Sbjct: 481 ANEKMKESLTTIPQVA 496

BLAST of CsGy5G029240 vs. NCBI nr
Match: KAE8648956.1 (hypothetical protein Csa_007904 [Cucumis sativus])

HSP 1 Score: 978 bits (2529), Expect = 0.0
Identity = 470/496 (94.76%), Postives = 485/496 (97.78%), Query Frame = 0

Query: 1   MAATFLTVGSLTSSSINNVGEQLPGCLSLKRVPAHLRFSNGYRTKAASNPNLKAVEVPRQ 60
           MAATFLTVGSLTSSSINNVGEQLPGCLSLKRVPAHLRFSNGYRTKAASNPNLKAVEVPRQ
Sbjct: 1   MAATFLTVGSLTSSSINNVGEQLPGCLSLKRVPAHLRFSNGYRTKAASNPNLKAVEVPRQ 60

Query: 61  WYNLVADLPVKPPPPLHPKTYEPIKPEDLSHLFPDELIKQEASTERYINIPDEVVDIYGL 120
           WYNLVADLPVKPPPPLHPKTYEPIKPEDLSHLFPDELIKQEASTERYINIPDEVVDIYGL
Sbjct: 61  WYNLVADLPVKPPPPLHPKTYEPIKPEDLSHLFPDELIKQEASTERYINIPDEVVDIYGL 120

Query: 121 WRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYNAQEGVKNVVTETG 180
           WRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYNAQEGVKNVVTETG
Sbjct: 121 WRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYNAQEGVKNVVTETG 180

Query: 181 AGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSDITDAGRKFL 240
           AGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRR+MMETWGAKVHPSPS+ITD GRKFL
Sbjct: 181 AGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRIMMETWGAKVHPSPSNITDVGRKFL 240

Query: 241 QVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQLEALGETP 300
           QVDPSS GSLGIAISEA E+A LN DT+YCLGSVLNHVLLHQTVIGEECLKQ+EALGE+P
Sbjct: 241 QVDPSSSGSLGIAISEATEIATLNVDTRYCLGSVLNHVLLHQTVIGEECLKQMEALGESP 300

Query: 301 DLIIGCTGGGSNFAGLSFPFLREKLAGNINPVIRAVEPAACPSLTKGVYAYDYGDTAGMT 360
           D++IGCTGGGSNFAGL+FPFLREKLAG +NPVIRA EPAACPSLTKGVYAYDYGDTAGMT
Sbjct: 301 DVVIGCTGGGSNFAGLAFPFLREKLAGKMNPVIRAAEPAACPSLTKGVYAYDYGDTAGMT 360

Query: 361 PLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFARS 420
           PLMKMHTLGHNFIPDPIHAGGLRYHG+APLISHVYNLGLLEAISLPQTECF+GA+QFARS
Sbjct: 361 PLMKMHTLGHNFIPDPIHAGGLRYHGIAPLISHVYNLGLLEAISLPQTECFRGAVQFARS 420

Query: 421 EGLIPAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGISDLSF 480
           EGLIPAPEPTHAIAATIREALRCR+TGESKVILTAM GHGHFDLP YEKYLQGGI DLSF
Sbjct: 421 EGLIPAPEPTHAIAATIREALRCRDTGESKVILTAMSGHGHFDLPVYEKYLQGGIRDLSF 480

Query: 481 ANEKIQESLTTIPQVA 496
           A EKIQESLT+IPQVA
Sbjct: 481 AKEKIQESLTSIPQVA 496

BLAST of CsGy5G029240 vs. NCBI nr
Match: XP_038890851.1 (tryptophan synthase beta chain 2 [Benincasa hispida])

HSP 1 Score: 974 bits (2518), Expect = 0.0
Identity = 476/497 (95.77%), Postives = 485/497 (97.59%), Query Frame = 0

Query: 1   MAATFLTVGSLTSSSINN--VGEQLPGCLSLKRVPAHLRFSNGYRTKAASNPNLKAVEVP 60
           MAATFL VGSLTSS INN  VGEQL G LSLKRVP HLRFSNGYRTKAASNPNLKAVEVP
Sbjct: 1   MAATFLAVGSLTSSGINNGRVGEQLSGYLSLKRVPRHLRFSNGYRTKAASNPNLKAVEVP 60

Query: 61  RQWYNLVADLPVKPPPPLHPKTYEPIKPEDLSHLFPDELIKQEASTERYINIPDEVVDIY 120
           RQWYNLVADLPVKPPPPLHPKT EPIKPEDLSHLFPDELIKQEAST++YINIPDEVVDIY
Sbjct: 61  RQWYNLVADLPVKPPPPLHPKTLEPIKPEDLSHLFPDELIKQEASTDQYINIPDEVVDIY 120

Query: 121 GLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYNAQEGVKNVVTE 180
           GLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQV+YN QEGVKNVVTE
Sbjct: 121 GLWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVYYNVQEGVKNVVTE 180

Query: 181 TGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSDITDAGRK 240
           TGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSD+TDAGRK
Sbjct: 181 TGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSDMTDAGRK 240

Query: 241 FLQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQLEALGE 300
           FLQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQ+EALGE
Sbjct: 241 FLQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQMEALGE 300

Query: 301 TPDLIIGCTGGGSNFAGLSFPFLREKLAGNINPVIRAVEPAACPSLTKGVYAYDYGDTAG 360
           +PD+IIGCTGGGSNFAGLSFPFLREKLAG INPVIRAVEPAACPSLTKGVYAYDYGDTAG
Sbjct: 301 SPDVIIGCTGGGSNFAGLSFPFLREKLAGKINPVIRAVEPAACPSLTKGVYAYDYGDTAG 360

Query: 361 MTPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFA 420
           MTPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFA
Sbjct: 361 MTPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFA 420

Query: 421 RSEGLIPAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGISDL 480
           RSEGLIPAPEPTHAIAATI+EALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGI+DL
Sbjct: 421 RSEGLIPAPEPTHAIAATIQEALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGITDL 480

Query: 481 SFANEKIQESLTTIPQV 495
           SFA EKIQESL TIPQV
Sbjct: 481 SFAKEKIQESLITIPQV 497

BLAST of CsGy5G029240 vs. NCBI nr
Match: XP_022939582.1 (uncharacterized protein LOC111445435 [Cucurbita moschata])

HSP 1 Score: 936 bits (2419), Expect = 0.0
Identity = 453/488 (92.83%), Postives = 469/488 (96.11%), Query Frame = 0

Query: 8   VGSLTSSSINNVGEQLPGCLSLKRVPAHLRFSNGYRTKAASNPNLKAVEVPRQWYNLVAD 67
           V SL SS IN       GCLSL+R+P HLRFSNGYRTKAASNPNLKAVEVPRQWYNLVAD
Sbjct: 2   VASLISSGING-----GGCLSLERIPRHLRFSNGYRTKAASNPNLKAVEVPRQWYNLVAD 61

Query: 68  LPVKPPPPLHPKTYEPIKPEDLSHLFPDELIKQEASTERYINIPDEVVDIYGLWRPTPLI 127
           LPVKPPPPLHPKT+EPIKPEDLS LFPDELIKQEAS ERYI+IPDEVVDIYGLWRPTPLI
Sbjct: 62  LPVKPPPPLHPKTFEPIKPEDLSPLFPDELIKQEASAERYIDIPDEVVDIYGLWRPTPLI 121

Query: 128 RAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYNAQEGVKNVVTETGAGQWGCS 187
           RAKRLEKLL TPARIYYKYEGVSPAGSHKPNSAVPQVWYN QEGVKNVVTETGAGQWGCS
Sbjct: 122 RAKRLEKLLGTPARIYYKYEGVSPAGSHKPNSAVPQVWYNVQEGVKNVVTETGAGQWGCS 181

Query: 188 LAFACSIFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSDITDAGRKFLQVDPSSP 247
           LAFACS+FGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPS++TD+GRKFLQVDPSSP
Sbjct: 182 LAFACSLFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSNVTDSGRKFLQVDPSSP 241

Query: 248 GSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQLEALGETPDLIIGCT 307
           GSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQ+EALGETPD+IIGCT
Sbjct: 242 GSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQMEALGETPDVIIGCT 301

Query: 308 GGGSNFAGLSFPFLREKLAGNINPVIRAVEPAACPSLTKGVYAYDYGDTAGMTPLMKMHT 367
           GGGSNFAGLSFPFLREKLAG I+PVIRAVEPAACPSLTKGVYAYDYGDTAGMTPLMKMHT
Sbjct: 302 GGGSNFAGLSFPFLREKLAGKISPVIRAVEPAACPSLTKGVYAYDYGDTAGMTPLMKMHT 361

Query: 368 LGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFARSEGLIPAP 427
           LGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEA+SLPQTECFQGAIQFARSEGLIPAP
Sbjct: 362 LGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAVSLPQTECFQGAIQFARSEGLIPAP 421

Query: 428 EPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGISDLSFANEKIQE 487
           EPTHAIA TIREALRCRETGESKVILTAMCGHGHFDL AYEKYLQGG++DLSFA EKI+E
Sbjct: 422 EPTHAIAETIREALRCRETGESKVILTAMCGHGHFDLAAYEKYLQGGMTDLSFAEEKIKE 481

Query: 488 SLTTIPQV 495
           SLTTIPQ+
Sbjct: 482 SLTTIPQL 484

BLAST of CsGy5G029240 vs. ExPASy TrEMBL
Match: A0A0A0KUJ4 (Tryptophan synthase OS=Cucumis sativus OX=3659 GN=Csa_5G643330 PE=3 SV=1)

HSP 1 Score: 1017 bits (2630), Expect = 0.0
Identity = 495/496 (99.80%), Postives = 496/496 (100.00%), Query Frame = 0

Query: 1   MAATFLTVGSLTSSSINNVGEQLPGCLSLKRVPAHLRFSNGYRTKAASNPNLKAVEVPRQ 60
           MAATFLTVGSLTSSSINNVGEQLPGCLSLKRVPAHLRFSNGYRTKAASNPNLK+VEVPRQ
Sbjct: 1   MAATFLTVGSLTSSSINNVGEQLPGCLSLKRVPAHLRFSNGYRTKAASNPNLKSVEVPRQ 60

Query: 61  WYNLVADLPVKPPPPLHPKTYEPIKPEDLSHLFPDELIKQEASTERYINIPDEVVDIYGL 120
           WYNLVADLPVKPPPPLHPKTYEPIKPEDLSHLFPDELIKQEASTERYINIPDEVVDIYGL
Sbjct: 61  WYNLVADLPVKPPPPLHPKTYEPIKPEDLSHLFPDELIKQEASTERYINIPDEVVDIYGL 120

Query: 121 WRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYNAQEGVKNVVTETG 180
           WRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYNAQEGVKNVVTETG
Sbjct: 121 WRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYNAQEGVKNVVTETG 180

Query: 181 AGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSDITDAGRKFL 240
           AGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSDITDAGRKFL
Sbjct: 181 AGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSDITDAGRKFL 240

Query: 241 QVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQLEALGETP 300
           QVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQLEALGETP
Sbjct: 241 QVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQLEALGETP 300

Query: 301 DLIIGCTGGGSNFAGLSFPFLREKLAGNINPVIRAVEPAACPSLTKGVYAYDYGDTAGMT 360
           DLIIGCTGGGSNFAGLSFPFLREKLAGNINPVIRAVEPAACPSLTKGVYAYDYGDTAGMT
Sbjct: 301 DLIIGCTGGGSNFAGLSFPFLREKLAGNINPVIRAVEPAACPSLTKGVYAYDYGDTAGMT 360

Query: 361 PLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFARS 420
           PLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFARS
Sbjct: 361 PLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFARS 420

Query: 421 EGLIPAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGISDLSF 480
           EGLIPAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGISDLSF
Sbjct: 421 EGLIPAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGISDLSF 480

Query: 481 ANEKIQESLTTIPQVA 496
           ANEKIQESLTTIPQVA
Sbjct: 481 ANEKIQESLTTIPQVA 496

BLAST of CsGy5G029240 vs. ExPASy TrEMBL
Match: A0A1S3CA62 (Tryptophan synthase OS=Cucumis melo OX=3656 GN=LOC103498574 PE=3 SV=1)

HSP 1 Score: 1008 bits (2605), Expect = 0.0
Identity = 486/496 (97.98%), Postives = 495/496 (99.80%), Query Frame = 0

Query: 1   MAATFLTVGSLTSSSINNVGEQLPGCLSLKRVPAHLRFSNGYRTKAASNPNLKAVEVPRQ 60
           MAATFLTVGSLTSSSINNVGEQLPGCLSLKRVP HLRFSNGYRTK+ASNPNLKAVE+PRQ
Sbjct: 1   MAATFLTVGSLTSSSINNVGEQLPGCLSLKRVPTHLRFSNGYRTKSASNPNLKAVEIPRQ 60

Query: 61  WYNLVADLPVKPPPPLHPKTYEPIKPEDLSHLFPDELIKQEASTERYINIPDEVVDIYGL 120
           WYNLVADLPVKPPPPLHPKTYEPIKPEDLSHLFPDELIKQEASTERYINIPDEVVDIYGL
Sbjct: 61  WYNLVADLPVKPPPPLHPKTYEPIKPEDLSHLFPDELIKQEASTERYINIPDEVVDIYGL 120

Query: 121 WRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYNAQEGVKNVVTETG 180
           WRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYNAQEGVKNVVTETG
Sbjct: 121 WRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYNAQEGVKNVVTETG 180

Query: 181 AGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSDITDAGRKFL 240
           AGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSD+TDAGRKFL
Sbjct: 181 AGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSDVTDAGRKFL 240

Query: 241 QVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQLEALGETP 300
           QVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQLEALGE+P
Sbjct: 241 QVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQLEALGESP 300

Query: 301 DLIIGCTGGGSNFAGLSFPFLREKLAGNINPVIRAVEPAACPSLTKGVYAYDYGDTAGMT 360
           DLIIGCTGGGSNFAGLSFPFLREKLAGNINPVIRAVEPAACPSLTKGVYAYDYGDTAGMT
Sbjct: 301 DLIIGCTGGGSNFAGLSFPFLREKLAGNINPVIRAVEPAACPSLTKGVYAYDYGDTAGMT 360

Query: 361 PLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFARS 420
           PLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEA+SLPQTECFQGAI+FARS
Sbjct: 361 PLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAVSLPQTECFQGAIKFARS 420

Query: 421 EGLIPAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGISDLSF 480
           EGLIPAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGI+DLSF
Sbjct: 421 EGLIPAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGITDLSF 480

Query: 481 ANEKIQESLTTIPQVA 496
           ANEK++ESLTTIPQVA
Sbjct: 481 ANEKMKESLTTIPQVA 496

BLAST of CsGy5G029240 vs. ExPASy TrEMBL
Match: A0A6J1FH74 (Tryptophan synthase OS=Cucurbita moschata OX=3662 GN=LOC111445435 PE=3 SV=1)

HSP 1 Score: 936 bits (2419), Expect = 0.0
Identity = 453/488 (92.83%), Postives = 469/488 (96.11%), Query Frame = 0

Query: 8   VGSLTSSSINNVGEQLPGCLSLKRVPAHLRFSNGYRTKAASNPNLKAVEVPRQWYNLVAD 67
           V SL SS IN       GCLSL+R+P HLRFSNGYRTKAASNPNLKAVEVPRQWYNLVAD
Sbjct: 2   VASLISSGING-----GGCLSLERIPRHLRFSNGYRTKAASNPNLKAVEVPRQWYNLVAD 61

Query: 68  LPVKPPPPLHPKTYEPIKPEDLSHLFPDELIKQEASTERYINIPDEVVDIYGLWRPTPLI 127
           LPVKPPPPLHPKT+EPIKPEDLS LFPDELIKQEAS ERYI+IPDEVVDIYGLWRPTPLI
Sbjct: 62  LPVKPPPPLHPKTFEPIKPEDLSPLFPDELIKQEASAERYIDIPDEVVDIYGLWRPTPLI 121

Query: 128 RAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYNAQEGVKNVVTETGAGQWGCS 187
           RAKRLEKLL TPARIYYKYEGVSPAGSHKPNSAVPQVWYN QEGVKNVVTETGAGQWGCS
Sbjct: 122 RAKRLEKLLGTPARIYYKYEGVSPAGSHKPNSAVPQVWYNVQEGVKNVVTETGAGQWGCS 181

Query: 188 LAFACSIFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSDITDAGRKFLQVDPSSP 247
           LAFACS+FGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPS++TD+GRKFLQVDPSSP
Sbjct: 182 LAFACSLFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSNVTDSGRKFLQVDPSSP 241

Query: 248 GSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQLEALGETPDLIIGCT 307
           GSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQ+EALGETPD+IIGCT
Sbjct: 242 GSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQMEALGETPDVIIGCT 301

Query: 308 GGGSNFAGLSFPFLREKLAGNINPVIRAVEPAACPSLTKGVYAYDYGDTAGMTPLMKMHT 367
           GGGSNFAGLSFPFLREKLAG I+PVIRAVEPAACPSLTKGVYAYDYGDTAGMTPLMKMHT
Sbjct: 302 GGGSNFAGLSFPFLREKLAGKISPVIRAVEPAACPSLTKGVYAYDYGDTAGMTPLMKMHT 361

Query: 368 LGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFARSEGLIPAP 427
           LGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEA+SLPQTECFQGAIQFARSEGLIPAP
Sbjct: 362 LGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAVSLPQTECFQGAIQFARSEGLIPAP 421

Query: 428 EPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGISDLSFANEKIQE 487
           EPTHAIA TIREALRCRETGESKVILTAMCGHGHFDL AYEKYLQGG++DLSFA EKI+E
Sbjct: 422 EPTHAIAETIREALRCRETGESKVILTAMCGHGHFDLAAYEKYLQGGMTDLSFAEEKIKE 481

Query: 488 SLTTIPQV 495
           SLTTIPQ+
Sbjct: 482 SLTTIPQL 484

BLAST of CsGy5G029240 vs. ExPASy TrEMBL
Match: A0A6J1JY44 (Tryptophan synthase OS=Cucurbita maxima OX=3661 GN=LOC111489338 PE=3 SV=1)

HSP 1 Score: 934 bits (2413), Expect = 0.0
Identity = 451/488 (92.42%), Postives = 469/488 (96.11%), Query Frame = 0

Query: 8   VGSLTSSSINNVGEQLPGCLSLKRVPAHLRFSNGYRTKAASNPNLKAVEVPRQWYNLVAD 67
           V S  SS IN       GCLS++R+P HLRFSNGYRTKAASNPNLKAVEVPRQWYNLVAD
Sbjct: 2   VASSISSGING-----GGCLSIERIPRHLRFSNGYRTKAASNPNLKAVEVPRQWYNLVAD 61

Query: 68  LPVKPPPPLHPKTYEPIKPEDLSHLFPDELIKQEASTERYINIPDEVVDIYGLWRPTPLI 127
           LPVKPPPPLHPKT+EPIKPEDLS LFPDELIKQEAS ERYI+IPDEVVDIYGLWRPTPLI
Sbjct: 62  LPVKPPPPLHPKTFEPIKPEDLSPLFPDELIKQEASAERYIDIPDEVVDIYGLWRPTPLI 121

Query: 128 RAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYNAQEGVKNVVTETGAGQWGCS 187
           RAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYN QEGVKNVVTETGAGQWGCS
Sbjct: 122 RAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYNVQEGVKNVVTETGAGQWGCS 181

Query: 188 LAFACSIFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSDITDAGRKFLQVDPSSP 247
           LAFACS+FGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPS++TD+GRKFLQVDPSSP
Sbjct: 182 LAFACSLFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSNVTDSGRKFLQVDPSSP 241

Query: 248 GSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQLEALGETPDLIIGCT 307
           GSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQ+EALGETPD+IIGCT
Sbjct: 242 GSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQMEALGETPDVIIGCT 301

Query: 308 GGGSNFAGLSFPFLREKLAGNINPVIRAVEPAACPSLTKGVYAYDYGDTAGMTPLMKMHT 367
           GGGSNFAGLSFPFLREKLAG I+PVIRAVEPAACPSLTKGVYAYDYGDTAGMTPLMKMHT
Sbjct: 302 GGGSNFAGLSFPFLREKLAGKISPVIRAVEPAACPSLTKGVYAYDYGDTAGMTPLMKMHT 361

Query: 368 LGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFARSEGLIPAP 427
           LGHNFIPDPIHAGGLRYHGMAPLISHVY+LGLLEA+SLPQTECFQGAIQFARSEGLIPAP
Sbjct: 362 LGHNFIPDPIHAGGLRYHGMAPLISHVYDLGLLEAVSLPQTECFQGAIQFARSEGLIPAP 421

Query: 428 EPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGISDLSFANEKIQE 487
           EPTHAIA TIREALRCRETGESKVILTAMCGHGHFDL AYEKYLQGG++DLSFA EKI+E
Sbjct: 422 EPTHAIAETIREALRCRETGESKVILTAMCGHGHFDLAAYEKYLQGGMTDLSFAEEKIKE 481

Query: 488 SLTTIPQV 495
           SLTTIPQ+
Sbjct: 482 SLTTIPQL 484

BLAST of CsGy5G029240 vs. ExPASy TrEMBL
Match: A0A6J1BXI7 (Tryptophan synthase OS=Momordica charantia OX=3673 GN=LOC111006607 PE=3 SV=1)

HSP 1 Score: 929 bits (2401), Expect = 0.0
Identity = 450/497 (90.54%), Postives = 474/497 (95.37%), Query Frame = 0

Query: 1   MAATFLTVGSLTSSSINN-VGEQLPGCLSLKRVPAHLRFSNGYRTKAASNPNLKAVEVPR 60
           MA+ FL   S TS +IN  VGE+LPG LSLKR P +LRFSNGYRT+AASN NLK VEVPR
Sbjct: 1   MASHFLVAASSTSPAINGRVGEELPGRLSLKRNPRNLRFSNGYRTRAASNRNLKPVEVPR 60

Query: 61  QWYNLVADLPVKPPPPLHPKTYEPIKPEDLSHLFPDELIKQEASTERYINIPDEVVDIYG 120
           +WYNLVADLPVKPPPPLHPKT++PIKP+DLS LFPD LI+QEAST+RYINIPDEVVDIYG
Sbjct: 61  RWYNLVADLPVKPPPPLHPKTFQPIKPQDLSPLFPDALIEQEASTDRYINIPDEVVDIYG 120

Query: 121 LWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYNAQEGVKNVVTET 180
           LWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHK NSAVPQVWYN QEGVKNVVTET
Sbjct: 121 LWRPTPLIRAKRLEKLLDTPARIYYKYEGVSPAGSHKANSAVPQVWYNVQEGVKNVVTET 180

Query: 181 GAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSDITDAGRKF 240
           GAGQWG +LAFACSIFG+GCEVWQVRASYDQKPYRR+MM+TWG KVHPSPSD+TDAGRK 
Sbjct: 181 GAGQWGSALAFACSIFGLGCEVWQVRASYDQKPYRRLMMQTWGVKVHPSPSDLTDAGRKL 240

Query: 241 LQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQLEALGET 300
           LQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQ+EALGE+
Sbjct: 241 LQVDPSSPGSLGIAISEAVEVAALNADTKYCLGSVLNHVLLHQTVIGEECLKQMEALGES 300

Query: 301 PDLIIGCTGGGSNFAGLSFPFLREKLAGNINPVIRAVEPAACPSLTKGVYAYDYGDTAGM 360
           PD+IIGCTGGGSNFAGLSFPFLREKLAG INP++RAVEPAACPSLTKGVYAYD+GDTAGM
Sbjct: 301 PDVIIGCTGGGSNFAGLSFPFLREKLAGKINPILRAVEPAACPSLTKGVYAYDFGDTAGM 360

Query: 361 TPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFAR 420
           TPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFAR
Sbjct: 361 TPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFAR 420

Query: 421 SEGLIPAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGISDLS 480
           SEGLI APEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGG++DLS
Sbjct: 421 SEGLIAAPEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQGGMTDLS 480

Query: 481 FANEKIQESLTTIPQVA 496
           FA EKIQESLTTIPQVA
Sbjct: 481 FAEEKIQESLTTIPQVA 497

BLAST of CsGy5G029240 vs. TAIR 10
Match: AT5G38530.1 (tryptophan synthase beta type 2 )

HSP 1 Score: 789.3 bits (2037), Expect = 1.8e-228
Identity = 377/474 (79.54%), Postives = 424/474 (89.45%), Query Frame = 0

Query: 27  LSLKRVPAH-LRFSNG--YRTKAA--SNPNLKAVEVPRQWYNLVADLPVKPPPPLHPKTY 86
           L+LKR      R SNG   R KAA  S  N   VE+P+QWYNLVADL VKPPPPLHPKT+
Sbjct: 32  LTLKRKSNQATRVSNGSSLRVKAALRSTHNKSVVEIPKQWYNLVADLSVKPPPPLHPKTF 91

Query: 87  EPIKPEDLSHLFPDELIKQEASTERYINIPDEVVDIYGLWRPTPLIRAKRLEKLLDTPAR 146
           EPIKPEDL+HLFP+ELIKQEA+ ER+I+IP+EV++IY LWRPTPLIRAKRLEKLL TPAR
Sbjct: 92  EPIKPEDLAHLFPNELIKQEATQERFIDIPEEVLEIYKLWRPTPLIRAKRLEKLLQTPAR 151

Query: 147 IYYKYEGVSPAGSHKPNSAVPQVWYNAQEGVKNVVTETGAGQWGCSLAFACSIFGIGCEV 206
           IY+KYEG SPAGSHKPN+AVPQ +YNA+EGVKNVVTETGAGQWG SLAFA S+FG+ CEV
Sbjct: 152 IYFKYEGGSPAGSHKPNTAVPQAYYNAKEGVKNVVTETGAGQWGSSLAFASSLFGLDCEV 211

Query: 207 WQVRASYDQKPYRRMMMETWGAKVHPSPSDITDAGRKFLQVDPSSPGSLGIAISEAVEVA 266
           WQV  SY  KPYRR+MM+TWGAKVHPSPSD+T+AGR+ L+ DPSSPGSLGIAISEAVEVA
Sbjct: 212 WQVANSYHTKPYRRLMMQTWGAKVHPSPSDLTEAGRRILESDPSSPGSLGIAISEAVEVA 271

Query: 267 ALNADTKYCLGSVLNHVLLHQTVIGEECLKQLEALGETPDLIIGCTGGGSNFAGLSFPFL 326
           A N DTKYCLGSVLNHVLLHQT+IGEEC++Q+E  GETPDLIIGCTGGGSNFAGLSFPF+
Sbjct: 272 ARNEDTKYCLGSVLNHVLLHQTIIGEECIQQMENFGETPDLIIGCTGGGSNFAGLSFPFI 331

Query: 327 REKLAGNINPVIRAVEPAACPSLTKGVYAYDYGDTAGMTPLMKMHTLGHNFIPDPIHAGG 386
           REKL G INPVIRAVEP+ACPSLTKGVYAYD+GDTAG+TPLMKMHTLGH+FIPDPIHAGG
Sbjct: 332 REKLKGKINPVIRAVEPSACPSLTKGVYAYDFGDTAGLTPLMKMHTLGHDFIPDPIHAGG 391

Query: 387 LRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFARSEGLIPAPEPTHAIAATIREAL 446
           LRYHGMAPLISHVY  G +EAIS+PQ ECFQGAIQFAR+EG+IPAPEPTHAIAATIREAL
Sbjct: 392 LRYHGMAPLISHVYEQGFMEAISIPQIECFQGAIQFARTEGIIPAPEPTHAIAATIREAL 451

Query: 447 RCRETGESKVILTAMCGHGHFDLPAYEKYLQGGISDLSFANEKIQESLTTIPQV 496
           RC+ETGE+KVIL AMCGHGHFDL +Y+KYL+G + DLSF+ EKI+ESL+ +P V
Sbjct: 452 RCKETGEAKVILMAMCGHGHFDLTSYDKYLKGELVDLSFSEEKIRESLSKVPHV 505

BLAST of CsGy5G029240 vs. TAIR 10
Match: AT5G54810.1 (tryptophan synthase beta-subunit 1 )

HSP 1 Score: 120.2 bits (300), Expect = 4.8e-27
Identity = 105/346 (30.35%), Postives = 147/346 (42.49%), Query Frame = 0

Query: 142 IYYKYEGVSPAGSHKPNSAVPQVWYNAQEGVKNVVTETGAGQWGCSLAFACSIFGIGCEV 201
           IY K E ++  G+HK N+AV Q     + G K ++ ETGAGQ G + A  C+ FG+ C +
Sbjct: 151 IYLKREDLNHTGAHKINNAVAQALLAKRLGKKRIIAETGAGQHGVATATVCARFGLECII 210

Query: 202 WQVRASYDQKPYRRMMMETWGAKVHPSPSDITDAGRKFLQVDPSSPGSLGIAISEAVEVA 261
           +      +++      M   GA+V                   S   +L  A SEA+   
Sbjct: 211 YMGAQDMERQALNVFRMRLLGAEVRGV---------------HSGTATLKDATSEAIRDW 270

Query: 262 ALNAD-TKYCLGSVLN------HVLLHQTVIGEECLKQ-LEALGETPDLIIGCTGGGSNF 321
             N + T Y LGSV         V     VIG+E  KQ LE  G  PD+++ C GGGSN 
Sbjct: 271 VTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKWGGKPDVLVACVGGGSNA 330

Query: 322 AGLSFPFLREKLAGNINPVIRAVEPAA--------CPSLTKGVYAYDYGDTAGMTPLMKM 381
            GL   F+ +     I      VE A           +LTKG    D G   G    +  
Sbjct: 331 MGLFHEFVNDTEVRMI-----GVEAAGFGLDSGKHAATLTKG----DVGVLHGAMSYLLQ 390

Query: 382 HTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFARSEGLIP 441
              G    P  I A GL Y G+ P  S   ++G  E  S+   E  +   + +R EG+IP
Sbjct: 391 DDDGQIIEPHSISA-GLDYPGVGPEHSFFKDMGRAEYYSITDEEALEAFKRVSRLEGIIP 450

Query: 442 APEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYL 472
           A E +HA+A   +    C    +   ++    G G  D+    KYL
Sbjct: 451 ALETSHALAYLEK---LCPTLSDGTRVVLNFSGRGDKDVQTVAKYL 468

BLAST of CsGy5G029240 vs. TAIR 10
Match: AT4G27070.1 (tryptophan synthase beta-subunit 2 )

HSP 1 Score: 117.9 bits (294), Expect = 2.4e-26
Identity = 104/347 (29.97%), Postives = 149/347 (42.94%), Query Frame = 0

Query: 142 IYYKYEGVSPAGSHKPNSAVPQVWYNAQEGVKNVVTETGAGQWGCSLAFACSIFGIGCEV 201
           IY K E ++  G+HK N+AV Q     + G K ++ ETGAGQ G + A  C+ FG+ C +
Sbjct: 156 IYLKREDLNHTGAHKINNAVAQALLAKRLGKKRIIAETGAGQHGVATATVCARFGLQCII 215

Query: 202 WQVRASYDQKPYRRMMMETWGAKVHPSPSDITDAGRKFLQVDPSSPGSLGIAISEAVEVA 261
           +      +++      M   GA+V                   S   +L  A SEA+   
Sbjct: 216 YMGAQDMERQALNVFRMRLLGAEVRGV---------------HSGTATLKDATSEAIRDW 275

Query: 262 ALNAD-TKYCLGSVLN------HVLLHQTVIGEECLKQ-LEALGETPDLIIGCTGGGSNF 321
             N + T Y LGSV         V     VIG+E  KQ +E  G  PD+++ C GGGSN 
Sbjct: 276 VTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQAMEKWGGKPDVLVACVGGGSNA 335

Query: 322 AGLSFPFLREKLAGNINPVIRAVEPAA--------CPSLTKGVYAYDYGDTAGMTPLMKM 381
            GL   F+ +     I      VE A           +LTKG    D G   G    +  
Sbjct: 336 MGLFHEFVDDTEVRMI-----GVEAAGFGLDSGKHAATLTKG----DVGVLHGAMSYLLQ 395

Query: 382 HTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLEAISLPQTECFQGAIQFARSEGLIP 441
              G    P  I A GL Y G+ P  S + ++G  E  S+   E  +   + +R EG+IP
Sbjct: 396 DDDGQIIEPHSISA-GLDYPGVGPEHSFLKDVGRAEYFSVTDEEALEAFKRVSRLEGIIP 455

Query: 442 APEPTHAIAATIREALRCRETGESKVILTAMCGHGHFDLPAYEKYLQ 473
           A E +HA+A   +    C    +   ++    G G  D+    KYL+
Sbjct: 456 ALETSHALAHLEK---LCPTLPDGARVVLNFSGRGDKDVQTAIKYLE 474

BLAST of CsGy5G029240 vs. TAIR 10
Match: AT5G28237.1 (Pyridoxal-5'-phosphate-dependent enzyme family protein )

HSP 1 Score: 114.8 bits (286), Expect = 2.0e-25
Identity = 102/333 (30.63%), Postives = 150/333 (45.05%), Query Frame = 0

Query: 122 RPTPLIRAKRLEK-------LLDTPARIYYKYEGVSPAGSHKPNSAVPQVWYNAQEGVKN 181
           R TPL  A+RL +        ++    IY K E +S  GSHK N+A+ Q   + + G   
Sbjct: 118 RETPLYFAERLTEHYKNIVPTIEGGPEIYLKREDLSHCGSHKINNALAQAMISRRLGCSR 177

Query: 182 VVTETGAGQWGCSLAFACSIFGIGCEVWQVRASYDQKPYRRMMMETWGAKVHPSPSDITD 241
           VV  TGAGQ G + A AC+   + C V+   A  +++ +  + M+  GA+V         
Sbjct: 178 VVAATGAGQHGVATAAACAKLSLECTVFMGAADIEKQSFNVLSMKLLGAQV--------- 237

Query: 242 AGRKFLQVDPSSPGSLGIAISEAVEVAALNADTKYCL-GSVLNH------VLLHQTVIGE 301
                     S  G+   A SEA+     N  T Y L G+V+        V   Q+VIG+
Sbjct: 238 ---------ISVEGTFKDASSEAIRNWVENLYTTYYLSGTVVGPHPCPIIVREFQSVIGK 297

Query: 302 ECLKQLEAL-GETPDLIIGCTGGGSNFAGLSFPFLREKLAGNINPVIRAVEPAACPSLTK 361
           E  +Q + L G  PD+++ C G GSN  GL   F+     G+ +  +  VE AA   L  
Sbjct: 298 ETRRQAKQLWGGKPDVLVACVGSGSNALGLFHEFV-----GDEDVRLVGVE-AAGLGLDS 357

Query: 362 GVYAY-----DYGDTAGMTPLMKMHTLGHNFIPDPIHAGGLRYHGMAPLISHVYNLGLLE 421
           G ++      D G   G    +     G    P  +   GL Y G+ P IS +   G  E
Sbjct: 358 GKHSATLAFGDVGVYHGSMSYLLQDDQGQILKPHSVGV-GLEYPGVGPEISFMKETGRAE 417

Query: 422 AISLPQTECFQGAIQFARSEGLIPAPEPTHAIA 435
             +    E  Q  ++ +R EG+IPA E +HA+A
Sbjct: 418 FYTATDEEAIQACMRLSRLEGIIPALEASHALA 425