CsGy5G029220 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy5G029220
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionProline dehydrogenase
LocationGy14Chr5: 32582769 .. 32586085 (+)
RNA-Seq ExpressionCsGy5G029220
SyntenyCsGy5G029220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATTAAACAAAAAAAAATTTAAAACATTCAAACAAAAAAAAAAAAAGAAGAAAAAAGAAAAGAAATTGAAAAAAAATTTCTAGAATTTTTTTTTTTATGGCTCAAGCTATTGTTTCAACCCAATCAAAGATCTCCAAAAGGGTATTTTTTTACGTTTTCAATCGGCCGTTGAACACCGCCGCCTCCTCCCCCGCCGCTGCCTTCACCGCGGCTACCGCTACCACCTCCCCACCGTCACCCCTTCATCTCAATCCTCCATCTACACCCACCGTTGATTTCACCGACTCTAGAGCCCTCTTCGGCTCGATTCCCACCTCCGACCTTCTACACGCCACCGCTACCCTCCACGCCGCCGCCGTCGGCCCCGTCGTCGACGTTGGAATGTGGGTTATGAATTCTAAATTGATGGACGTTGAGTTGTTTCGTGATGTGGTTTTGGGTACGGTTAAGCATAGTTTTTACCGGCATTTTTGTGCCGGTGAGGACGATACGTCCGTCGCTAAAACCGTTCGACGGTTGCACGATGTTGGGTTGAGAAGTATGCTTGATTATGCTCTTGAATATGCCGATGACGAAGCCTCCTGTGATCGGAATCTTGATGGCTTTCTTTCTACCGTCGAAGCAACTAAATCCCTCCCATCTGGTTCTGTAAGTACTTCTCTTAACTAATTTCTTGTTTGTTCTTCCTAACTTTTGATTCATTTCTTAAATTTTTCTATTTTAATCGCTAATTTTGATTCGATTTACAAACTTTCATGTTTGTTCTAAGTTTTTTCCGTTAATTTTTCTTTAAAACGTAACATTCATATTATCATTTCATTGTCCAACATATGAGTACTCAATCAATACCATCTTTGCTTTTTTCTAAAAGAAAAAAAAAAAAACTTTTTACTTAATTAATGCAAACTCTAACTATATATCTACATCAACCAATAAATAAATTTCCTCAAAGTCTACATTAACCCATTAAATATAAATTCTCTCATTGTTTCCATCTTTAAATACTAAATAATAATTAAAAAAAAAGGCATTTAGAATTAAATTAAATTGTATATTTATTTAAATAATATTAAACTTTAACTTAAAAATTACAACATTTGTATTCTTCGATAAGCACTGACTTACGTGTGCAAGAAGTTATATATATAAAGAAAAATGACTATTAGAGGCATATTTCTCAATTTAACTTGCTAGAAAGTTAGAAACGTAAATATTTTTTTTCTTATCATCTCAGAATATTTAAGCTGATTTATTGACATTTTAAAAAAGTTATTATTCTTTTTAAGTATATGGTAGTAATTAAAAACGTTACAAAATAATAAAGAATAATTCTTTTTGGTAATAAAATGAATTTTCTGGAATAGTCAACGGTCAATGATCATAGCATAAATTTCGATAAGATTTTGCTTCCACGTCGGTATGAATCATACATTGTGAGTATGAATAGTAGATTGCCACGTGGCTATTTGAGGGCTTTATACTCAAGTGACGGTGTGTGAATTTCCACCAATTTATATGGAGACTGAAAATGGAATCCTCTTCCTAAGTTTCTTTTTCATCTAAATCTTCTTCTCTTGTGAAAAGGAAAAGTTGACCAAGCAACTAGAATAAAACAAAAGAAAGGGCATTATGAACACATGATTACTGATAAAATTTTATCTAGTATTGTTTTCAACTTTTCACAGTTACCAATGTTACTGATATAGAGATTGAGCTTGTTCAACTTTCCAGTTTCTTTGCATGGTTTATTGACTTCATCAAAAGGAGTTTGGTTTGTTTGGATTGATGTGACATGTTTTGAATTTTCAATTCACAATGGACTGATGGAAGTGTTTGTGTTTTATTGATCATAGGCGAGTTTTGTGGTGACAAAAGTGAGTGCGATTTGTCCATTGAGATTGCTGGAGAGAGTTAGTGATTTGTTAAGATGGCAACAAAAGAACCCGAGTTTCAATCTTCCATGGAAGCTTCAAACTTTGCCAATTTTCTCTGAATCAAGTCCACTTTATCATACTCTTGAACAACCAGAACCCTTGACACGTGAAGAAGAAAAGAGTCTCCAAATGAGCCACGAAAGGCTCATGAAAATTTGCCAAAGTTGTGTTGACGCCAATGTTCCTTTAGCCATTGATGCTGAACATACTAAAGTTCAACCAGCCATTGATTACTTCACTTATTCAGCTGCCATTATTCACAACAAAGACCGTAATCCTATAGTTTATGGAACCATTCAAGCTTATCTAAAAGACGCAAAAGATAGGCTTTTGTTAGCTAACAAAGAGGCTTCCAAGTTGAAGGTTCCACTTGGGATTAAGCTTGTGAGAGGTGCTTATATGTCAAGTGAATCCAAACTTGCTTCTTCTCTTGGCTTTGAATCTCCAATTCACGATACCATTCAAGATACACATTCGTGTTACAATACTTGTGCTTCTTTCTTGCTTGACGACATCGCCAAGGGTTCGACCGGAGCTATTCTTGCAACCCATAATGTTGAATCAGGTAATGATCATCACAGAGTGTACACACACAATCATGCTTTTAGGTTCTTCAAAGTATTTAGTTTTCTGATTTTAAAACATTGTGTACGTGGTGTTTTGAGTAGGAAAACTGGCAGCTAGCAGAGCATACGAGATAGGGATTGGAAAGTTGAAGCAAAAGCTTGAGTTTGCTCAATTGTATGGAATGTCAGAAGCACTTTCTTTTGGGTTGAGAAATGCAGGGTTTCAAGTGAGTAAATACATGCCATTTGGACCTGTGGATATGGTAATGCCATACCTTTTGAGGAGAGCTGAGGAGAATAGAGGTCTCTTATCAGCTTCAAATTTGGATAGAGAACTAATGAGGTAATTATGAACTTGTGGTCTTCAAATTAAAAGTTTGGATTCAACTAACAAAAAATTTGACAAGTTTTTAAAATGGTTTTTGATTCTTATACAGAAAGGAGTTGGGAAGAAGGATGAAAGAGTACATAAGCTAAAAGAAAAAGAAAGGGCTTTCTAGGAATACTTCAACAAACATACAACAAAAACCAAGATTATTATTTGCAATTTTTTGTTTATAGGATTAGACATACTTTTAAACTTTACTCCTTTCCTTCTGTTACTATCCACATGTGTTTTCTACTTTTAAATTTGTATCATTATCTATTCTACCACTCAAATAATAGGACCTTTATAATTGAATTAGTAAGTTGTATGTAAGTTGTTGTGTCTCCATCTCATGTAAAAACAAATTAGATGATGATATATAAGAATAAAAATTTAGTTGTCACAAAAAGAACAAAATTGAAAGTGATCCAAAATTGACATCCAAACTCC

mRNA sequence

AATTAAACAAAAAAAAATTTAAAACATTCAAACAAAAAAAAAAAAAGAAGAAAAAAGAAAAGAAATTGAAAAAAAATTTCTAGAATTTTTTTTTTTATGGCTCAAGCTATTGTTTCAACCCAATCAAAGATCTCCAAAAGGGTATTTTTTTACGTTTTCAATCGGCCGTTGAACACCGCCGCCTCCTCCCCCGCCGCTGCCTTCACCGCGGCTACCGCTACCACCTCCCCACCGTCACCCCTTCATCTCAATCCTCCATCTACACCCACCGTTGATTTCACCGACTCTAGAGCCCTCTTCGGCTCGATTCCCACCTCCGACCTTCTACACGCCACCGCTACCCTCCACGCCGCCGCCGTCGGCCCCGTCGTCGACGTTGGAATGTGGGTTATGAATTCTAAATTGATGGACGTTGAGTTGTTTCGTGATGTGGTTTTGGGTACGGTTAAGCATAGTTTTTACCGGCATTTTTGTGCCGGTGAGGACGATACGTCCGTCGCTAAAACCGTTCGACGGTTGCACGATGTTGGGTTGAGAAGTATGCTTGATTATGCTCTTGAATATGCCGATGACGAAGCCTCCTGTGATCGGAATCTTGATGGCTTTCTTTCTACCGTCGAAGCAACTAAATCCCTCCCATCTGGTTCTGCGAGTTTTGTGGTGACAAAAGTGAGTGCGATTTGTCCATTGAGATTGCTGGAGAGAGTTAGTGATTTGTTAAGATGGCAACAAAAGAACCCGAGTTTCAATCTTCCATGGAAGCTTCAAACTTTGCCAATTTTCTCTGAATCAAGTCCACTTTATCATACTCTTGAACAACCAGAACCCTTGACACGTGAAGAAGAAAAGAGTCTCCAAATGAGCCACGAAAGGCTCATGAAAATTTGCCAAAGTTGTGTTGACGCCAATGTTCCTTTAGCCATTGATGCTGAACATACTAAAGTTCAACCAGCCATTGATTACTTCACTTATTCAGCTGCCATTATTCACAACAAAGACCGTAATCCTATAGTTTATGGAACCATTCAAGCTTATCTAAAAGACGCAAAAGATAGGCTTTTGTTAGCTAACAAAGAGGCTTCCAAGTTGAAGGTTCCACTTGGGATTAAGCTTGTGAGAGGTGCTTATATGTCAAGTGAATCCAAACTTGCTTCTTCTCTTGGCTTTGAATCTCCAATTCACGATACCATTCAAGATACACATTCGTGTTACAATACTTGTGCTTCTTTCTTGCTTGACGACATCGCCAAGGGTTCGACCGGAGCTATTCTTGCAACCCATAATGTTGAATCAGGAAAACTGGCAGCTAGCAGAGCATACGAGATAGGGATTGGAAAGTTGAAGCAAAAGCTTGAGTTTGCTCAATTGTATGGAATGTCAGAAGCACTTTCTTTTGGGTTGAGAAATGCAGGGTTTCAAGTGAGTAAATACATGCCATTTGGACCTGTGGATATGGTAATGCCATACCTTTTGAGGAGAGCTGAGGAGAATAGAGGTCTCTTATCAGCTTCAAATTTGGATAGAGAACTAATGAGAAAGGAGTTGGGAAGAAGGATGAAAGAGTACATAAGCTAAAAGAAAAAGAAAGGGCTTTCTAGGAATACTTCAACAAACATACAACAAAAACCAAGATTATTATTTGCAATTTTTTGTTTATAGGATTAGACATACTTTTAAACTTTACTCCTTTCCTTCTGTTACTATCCACATGTGTTTTCTACTTTTAAATTTGTATCATTATCTATTCTACCACTCAAATAATAGGACCTTTATAATTGAATTAGTAAGTTGTATGTAAGTTGTTGTGTCTCCATCTCATGTAAAAACAAATTAGATGATGATATATAAGAATAAAAATTTAGTTGTCACAAAAAGAACAAAATTGAAAGTGATCCAAAATTGACATCCAAACTCC

Coding sequence (CDS)

ATGGCTCAAGCTATTGTTTCAACCCAATCAAAGATCTCCAAAAGGGTATTTTTTTACGTTTTCAATCGGCCGTTGAACACCGCCGCCTCCTCCCCCGCCGCTGCCTTCACCGCGGCTACCGCTACCACCTCCCCACCGTCACCCCTTCATCTCAATCCTCCATCTACACCCACCGTTGATTTCACCGACTCTAGAGCCCTCTTCGGCTCGATTCCCACCTCCGACCTTCTACACGCCACCGCTACCCTCCACGCCGCCGCCGTCGGCCCCGTCGTCGACGTTGGAATGTGGGTTATGAATTCTAAATTGATGGACGTTGAGTTGTTTCGTGATGTGGTTTTGGGTACGGTTAAGCATAGTTTTTACCGGCATTTTTGTGCCGGTGAGGACGATACGTCCGTCGCTAAAACCGTTCGACGGTTGCACGATGTTGGGTTGAGAAGTATGCTTGATTATGCTCTTGAATATGCCGATGACGAAGCCTCCTGTGATCGGAATCTTGATGGCTTTCTTTCTACCGTCGAAGCAACTAAATCCCTCCCATCTGGTTCTGCGAGTTTTGTGGTGACAAAAGTGAGTGCGATTTGTCCATTGAGATTGCTGGAGAGAGTTAGTGATTTGTTAAGATGGCAACAAAAGAACCCGAGTTTCAATCTTCCATGGAAGCTTCAAACTTTGCCAATTTTCTCTGAATCAAGTCCACTTTATCATACTCTTGAACAACCAGAACCCTTGACACGTGAAGAAGAAAAGAGTCTCCAAATGAGCCACGAAAGGCTCATGAAAATTTGCCAAAGTTGTGTTGACGCCAATGTTCCTTTAGCCATTGATGCTGAACATACTAAAGTTCAACCAGCCATTGATTACTTCACTTATTCAGCTGCCATTATTCACAACAAAGACCGTAATCCTATAGTTTATGGAACCATTCAAGCTTATCTAAAAGACGCAAAAGATAGGCTTTTGTTAGCTAACAAAGAGGCTTCCAAGTTGAAGGTTCCACTTGGGATTAAGCTTGTGAGAGGTGCTTATATGTCAAGTGAATCCAAACTTGCTTCTTCTCTTGGCTTTGAATCTCCAATTCACGATACCATTCAAGATACACATTCGTGTTACAATACTTGTGCTTCTTTCTTGCTTGACGACATCGCCAAGGGTTCGACCGGAGCTATTCTTGCAACCCATAATGTTGAATCAGGAAAACTGGCAGCTAGCAGAGCATACGAGATAGGGATTGGAAAGTTGAAGCAAAAGCTTGAGTTTGCTCAATTGTATGGAATGTCAGAAGCACTTTCTTTTGGGTTGAGAAATGCAGGGTTTCAAGTGAGTAAATACATGCCATTTGGACCTGTGGATATGGTAATGCCATACCTTTTGAGGAGAGCTGAGGAGAATAGAGGTCTCTTATCAGCTTCAAATTTGGATAGAGAACTAATGAGAAAGGAGTTGGGAAGAAGGATGAAAGAGTACATAAGCTAA

Protein sequence

MAQAIVSTQSKISKRVFFYVFNRPLNTAASSPAAAFTAATATTSPPSPLHLNPPSTPTVDFTDSRALFGSIPTSDLLHATATLHAAAVGPVVDVGMWVMNSKLMDVELFRDVVLGTVKHSFYRHFCAGEDDTSVAKTVRRLHDVGLRSMLDYALEYADDEASCDRNLDGFLSTVEATKSLPSGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLEQPEPLTREEEKSLQMSHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNKDRNPIVYGTIQAYLKDAKDRLLLANKEASKLKVPLGIKLVRGAYMSSESKLASSLGFESPIHDTIQDTHSCYNTCASFLLDDIAKGSTGAILATHNVESGKLAASRAYEIGIGKLKQKLEFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRELMRKELGRRMKEYIS*
Homology
BLAST of CsGy5G029220 vs. ExPASy Swiss-Prot
Match: P92983 (Proline dehydrogenase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=POX1 PE=1 SV=2)

HSP 1 Score: 525.4 bits (1352), Expect = 6.9e-148
Identity = 269/470 (57.23%), Postives = 347/470 (73.83%), Query Frame = 0

Query: 24  PLNTAASSPAAAFTAATATTSPPSPLHLNPPSTPT---VDFTDSRALFGSIPTSDLLHAT 83
           P    AS+       +    +P  PLH   P+  +   +D +D   LF SIPTSDLL +T
Sbjct: 25  PPTVTASTAVVPEILSFGQQAPEPPLHHPKPTEQSHDGLDLSDQARLFSSIPTSDLLRST 84

Query: 84  ATLHAAAVGPVVDVGMWVMNSKLMDVELFRDVVLGTVKHSFYRHFCAGEDDTSVAKTVRR 143
           A LHAAA+GP+VD+G WVM+SKLMD  + R +VLG VK +FY HFCAGED  + A+ VR 
Sbjct: 85  AVLHAAAIGPMVDLGTWVMSSKLMDASVTRGMVLGLVKSTFYDHFCAGEDADAAAERVRS 144

Query: 144 LHD-VGLRSMLDYALEYADDEASCDRNLDGFLSTVEATKSLPSGSASFVVTKVSAICPLR 203
           +++  GL+ ML Y +E+ADD  SCD N+  F+ T+EA KSLP+   S VV K++AICP+ 
Sbjct: 145 VYEATGLKGMLVYGVEHADDAVSCDDNMQQFIRTIEAAKSLPTSHFSSVVVKITAICPIS 204

Query: 204 LLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLEQPEPLTREEEKSLQMSHER 263
           LL+RVSDLLRW+ K+P+F L WKL++ P+FSESSPLYHT  +PEPLT EEE+ L+ +H R
Sbjct: 205 LLKRVSDLLRWEYKSPNFKLSWKLKSFPVFSESSPLYHTNSEPEPLTAEEERELEAAHGR 264

Query: 264 LMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNKDRN-PIVYGTIQAYLKDAK 323
           + +IC+ C ++NVPL IDAE T +QPAIDY  YS+AI+ N D++ PIVY TIQAYL+DA 
Sbjct: 265 IQEICRKCQESNVPLLIDAEDTILQPAIDYMAYSSAIMFNADKDRPIVYNTIQAYLRDAG 324

Query: 324 DRLLLANKEASKLKVPLGIKLVRGAYMSSESKLASSLGFESPIHDTIQDTHSCYNTCASF 383
           +RL LA + A K  VP+G KLVRGAYMSSE+ LA SLG +SP+HDTIQDTHSCYN C +F
Sbjct: 325 ERLHLAVQNAEKENVPMGFKLVRGAYMSSEASLADSLGCKSPVHDTIQDTHSCYNDCMTF 384

Query: 384 LLDDIAKGS-TGAILATHNVESGKLAASRAYEIGIGKLKQKLEFAQLYGMSEALSFGLRN 443
           L++  + GS  G +LATHN +SG+LA+ +A ++GI K   K+EFAQLYGMS+ALSFGL+ 
Sbjct: 385 LMEKASNGSGFGVVLATHNADSGRLASRKASDLGIDKQNGKIEFAQLYGMSDALSFGLKR 444

Query: 444 AGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRELMRKELGRRM 488
           AGF VSKYMPFGPV   +PYLLRRA ENRG+++    DR+LMR EL RR+
Sbjct: 445 AGFNVSKYMPFGPVATAIPYLLRRAYENRGMMATGAHDRQLMRMELKRRL 494

BLAST of CsGy5G029220 vs. ExPASy Swiss-Prot
Match: Q6NKX1 (Proline dehydrogenase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=POX2 PE=2 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 4.5e-147
Identity = 263/450 (58.44%), Postives = 336/450 (74.67%), Query Frame = 0

Query: 42  TTSPPSPLHLNPPSTPT-VDFTDSRALFGSIPTSDLLHATATLHAAAVGPVVDVGMWVMN 101
           TT  P  L  + P     +D +D   LF S+P S LL +TA LHA ++GP+VD+G W+M+
Sbjct: 24  TTIIPEILSFDQPKPEVDLDLSDQARLFASVPISTLLRSTAILHATSIGPMVDLGSWLMS 83

Query: 102 SKLMDVELFRDVVLGTVKHSFYRHFCAGEDDTSVAKTVRRLHD-VGLRSMLDYALEYADD 161
           SKLMD  + RD+VL  VK +FY HFCAGED  + A+ V  +++  GL+ ML Y +E+A+D
Sbjct: 84  SKLMDTTVTRDLVLRIVKGTFYDHFCAGEDAAAAARRVSSVYESTGLKGMLVYGVEHAED 143

Query: 162 EASCDRNLDGFLSTVEATKSLPSGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNL 221
             +CD N+  F+ TVEA K+LPS   S VV K++AICP+ +L+RVSDLLRWQ KNP+F L
Sbjct: 144 GGACDENIQKFIETVEAAKTLPSSHLSSVVVKITAICPMNVLKRVSDLLRWQYKNPNFKL 203

Query: 222 PWKLQTLPIFSESSPLYHTLEQPEPLTREEEKSLQMSHERLMKICQSCVDANVPLAIDAE 281
           PWKL + P+FS  SPLYHT  +PEPLT EEE+ L+ +HERL  +C  C ++NVPL IDAE
Sbjct: 204 PWKLNSFPVFSGLSPLYHTTSEPEPLTVEEERELEKAHERLKSVCLRCQESNVPLLIDAE 263

Query: 282 HTKVQPAIDYFTYSAAIIHNKDRN-PIVYGTIQAYLKDAKDRLLLANKEASKLKVPLGIK 341
            T +QPAIDY  Y +AI+ N D++ PIVY TIQAYLKDA +RL LA +E+ K+ VP+G K
Sbjct: 264 DTILQPAIDYMAYWSAIMFNSDKDRPIVYNTIQAYLKDAGERLHLALRESEKMNVPIGFK 323

Query: 342 LVRGAYMSSESKLASSLGFESPIHDTIQDTHSCYNTCASFLLDDIAKGS-TGAILATHNV 401
           LVRGAYMSSE+KLA SLG++SP+HDTIQ+TH CYN C SFL++  + GS    ILATHN 
Sbjct: 324 LVRGAYMSSEAKLADSLGYKSPVHDTIQNTHDCYNDCMSFLMEKASNGSGIAVILATHNT 383

Query: 402 ESGKLAASRAYEIGIGKLKQKLEFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPY 461
           +SGKL A +A E+GI K   K+EFAQLYGMS+ALSFGL+ AGF VSKYMP+GPVD  +PY
Sbjct: 384 DSGKLGARKASELGINKENGKIEFAQLYGMSDALSFGLKRAGFNVSKYMPYGPVDTAIPY 443

Query: 462 LLRRAEENRGLLSASNLDRELMRKELGRRM 488
           L+RRA ENRG++S   LDR+LMRKEL RR+
Sbjct: 444 LIRRAYENRGMMSTGALDRQLMRKELKRRV 473

BLAST of CsGy5G029220 vs. ExPASy Swiss-Prot
Match: Q86H28 (Proline dehydrogenase 1, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=prodh PE=3 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 7.9e-51
Identity = 148/446 (33.18%), Postives = 228/446 (51.12%), Query Frame = 0

Query: 63  DSRALFGSIPTSDLLHATATLHAAAVGPVVDVGMWVMNSKLMDVELFRDVVLGT-----V 122
           D+  L+ S  T +L      L   ++  + D     +N       LF  + L       +
Sbjct: 145 DTSKLYVSKSTGELFFTFMILKVCSINFISDNSQKFLN-------LFEKLGLSKPLNFFI 204

Query: 123 KHSFYRHFCAGEDDTSVAKTVRRLHDVGLRSMLDYAL-EYADDEASCDRNLDGFLSTVEA 182
           K+SF++ FCAGE          +L+ +G+ ++LDYA+ E A      D   +    T+  
Sbjct: 205 KYSFFKQFCAGETIRETEIFTEKLNKLGIGTILDYAIEELAGSSEGFDSVAENICETIRV 264

Query: 183 TKSLPSGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLY 242
               P+ S S V  K + +    +LE+++ L+         N+   +  LPI S +SPL 
Sbjct: 265 AAKNPTNSFSCV--KFTGLVTPSVLEKMNTLVS--------NVTTNVSELPIESFNSPLD 324

Query: 243 HTLEQ--------PEP-LTREEEKSLQMSHERLMKICQSCVDANVPLAIDAEHTKVQPAI 302
             L Q         EP LT ++ K ++    R+ KI Q C    VP+ +DAE +  Q AI
Sbjct: 325 FYLNQSSSLMKQGSEPLLTSKDIKEIKEFFNRMDKIFQLCHQRGVPILVDAEQSYYQVAI 384

Query: 303 DYFTYSAAIIHNKDRNPIVYGTIQAYLKDAKDRLL--LANKEASKLKVPLGIKLVRGAYM 362
            + T S +I +NK++ PI+Y T Q YL +  + L        + K    LG K+VRGAYM
Sbjct: 385 HHLTMSYSIKYNKEK-PIIYNTYQMYLVNGMNVLKQHFELSSSQKFNFKLGAKIVRGAYM 444

Query: 363 SSESKLASSLGFESPIHDTIQDTHSCYNTCASFLLDDIAK--GSTGAILATHNVESGKLA 422
            +ES+ +  L  E+P+  TIQDTH  YNT   FLL+ I     S G ++A+HN +S  L 
Sbjct: 445 VTESERSQRLSTENPVLPTIQDTHKSYNTALDFLLNQIKSDPNSIGLMIASHNEDSINLG 504

Query: 423 ASRAYEIGIGKLKQKLEFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAE 482
                +  I      ++F QL+GM++ LSF L +   ++ KY+PFGPV+ V+PYL+RR  
Sbjct: 505 TKLIKQYKIDPTNPNIQFGQLFGMADFLSFNLVDQHQRIFKYVPFGPVEEVLPYLIRRMH 564

Query: 483 ENRGLLSASNLDREL--MRKELGRRM 488
           EN+G +  SN D+EL  ++KE+ RR+
Sbjct: 565 ENKGFI-GSNSDKELFYLKKEIKRRL 571

BLAST of CsGy5G029220 vs. ExPASy Swiss-Prot
Match: O45228 (Proline dehydrogenase 1, mitochondrial OS=Caenorhabditis elegans OX=6239 GN=prdh-1 PE=2 SV=2)

HSP 1 Score: 167.9 bits (424), Expect = 2.8e-40
Identity = 140/549 (25.50%), Postives = 240/549 (43.72%), Query Frame = 0

Query: 59  VDFTDSRALFGSIPTSDLLHATATLHAAAVGPVVDVGMWVMNSKLMDVELFRDVVLGTVK 118
           + F +++  F S   ++L+ A   L    +  +V+    ++N+  M   L +++   T+K
Sbjct: 59  LSFENTKEAFKSKSNTELVRALVVLRLCGIQTLVNQNQIILNT--MRRVLGKNLFKKTLK 118

Query: 119 HSFYRHFCAGEDDTSVAKTVRRLHDVGLRSMLDYALE----------------------- 178
           ++F+ HF AGE +  V   V +L + G++S+LDY++E                       
Sbjct: 119 NTFFGHFVAGETEEEVRHVVEKLRNYGVKSILDYSVEADITSQEATDKTVKGTSVATVKP 178

Query: 179 --------------------------------------YADDEASCDRNLDGFLSTVEAT 238
                                                 + + E  CD+N D F  ++ A 
Sbjct: 179 AAMTPVVDAKTLETTRERYTVHEEFGDRRQGVSSARTYFYEGEEQCDKNRDIFKDSINAV 238

Query: 239 KSLPSGSASFVVTKVSAICPLRLLERVSDLLRWQQ---------------KNPSFNLPWK 298
            S  + +  FV  K++A+   +LL ++S+ +   Q               +  S     +
Sbjct: 239 AS-ATKNEGFVAVKITALGRPQLLLKLSEAIVQTQNFFKALTGGMSLQEGRLTSQEFYKR 298

Query: 299 LQTLPIFSESSPL--------------------YHTLEQP-----------------EP- 358
           L  L + +++  +                     H L+                   EP 
Sbjct: 299 LGELGVKTDTESVKKFFDEVDFDSDGIVDLHGWNHILDDHVKLGQLFQVLNIKTGSLEPL 358

Query: 359 ---LTREEEKSLQMSHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNKD 418
              L+ EEE+  +    R + + +  ++  V + +DAE T +QPAI   T      +NK 
Sbjct: 359 IQNLSNEEEQEFRNMVRRTLDVAEYAIEKGVRIMVDAEQTYLQPAISKITIEMMKKYNKG 418

Query: 419 RNPIVYGTIQAYLKDAKDRLLLANKEASKLKVPLGIKLVRGAYMSSESKLASSLGFESPI 478
           R  I + T QAYLK     +    + A +     G KLVRGAYM  E   A ++G+E PI
Sbjct: 419 RGNI-FNTYQAYLKGTLQNMEADMQVARREGWHFGAKLVRGAYMEQERARAKAIGYEDPI 478

Query: 479 HDTIQDTHSCYNTCASFLLDDI---AKGSTGAILATHNVESGKLAASRAYEIGIGKLKQK 488
           +D  + T   Y +C + + D++    K +   ++A+HN ++ + A +   E  I   ++ 
Sbjct: 479 NDNFEATSKMYESCLTRIADEVHRRGKTNVSVMVASHNEDTVRFALNLMKEKCISPSERV 538

BLAST of CsGy5G029220 vs. ExPASy Swiss-Prot
Match: A6QQ74 (Hydroxyproline dehydrogenase OS=Bos taurus OX=9913 GN=PRODH2 PE=2 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 1.5e-38
Identity = 121/386 (31.35%), Postives = 193/386 (50.00%), Query Frame = 0

Query: 117 VKHSFYRHFCAGEDDTSVAKTVRRLHDVGLRSMLDYALEYADD------EASCDRNLDGF 176
           ++ S Y  F AGE    V   V +L ++GLR +L    E   D      EA  + NL   
Sbjct: 76  LRASIYGQFVAGETAEEVRSCVLQLQNLGLRPLLAVPTEEEPDSAVKTGEAWYEGNLSAM 135

Query: 177 LSTVEATKSL-----PSGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQT 236
           L  V+ ++ L     P+G+A  +  K++A+   RL ++++  +R     P  +L    + 
Sbjct: 136 LRCVDLSRGLLETPDPTGNA-LMQLKMTALMSTRLCKQLTSWVR----RPGDSLELSPER 195

Query: 237 LPIFSESSPLYHTLEQPEPLTREEEKSLQMSHERLMKICQSCVDANVPLAIDAEHTKVQP 296
           L    +S        Q   L  E+ + LQ S  RL ++ Q      V L +DAE+T + P
Sbjct: 196 LAEAMDSGQDL----QVSCLNTEQTRHLQASLSRLHRVVQHARAQRVRLLVDAEYTSLNP 255

Query: 297 AIDYFTYSAAIIHNK--DRNPIVYGTIQAYLKDAKDRLLLANKEASKLKVPLGIKLVRGA 356
           A+     + A   N   +  P V+ T QAYLKD  +RL    + A +  +  G+KLVRGA
Sbjct: 256 ALSLLVAALATRWNSSGEGGPWVWNTYQAYLKDTYERLRWDAEAADRAGLAFGVKLVRGA 315

Query: 357 YMSSESKLASSLGFESPIHDTIQDTHSCYNTCASFLLDDIAKGS--TGAILATHNVESGK 416
           Y+  E + A   G E P     + T   Y+ C   +L  ++        ++A+HN +S +
Sbjct: 316 YLDKERETARLQGTEDPTQPDYEATSQSYSRCLELMLTQVSHRGPMCHLMVASHNEDSVR 375

Query: 417 LAASRAYEIGIGKLKQKLEFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRR 476
            A  R +E+GI      + F QL GM + +S  L  AG+ V K +P+G ++ V+PYL+RR
Sbjct: 376 QATKRMWELGIPP-DGPVCFGQLLGMCDHVSLALGQAGYAVYKSIPYGSLEEVIPYLIRR 435

Query: 477 AEENRGLLSASNLDRELMRKELGRRM 488
           A+ENR +L  +  ++EL+ +EL RR+
Sbjct: 436 AQENRSVLRGARREQELLSQELRRRL 451

BLAST of CsGy5G029220 vs. NCBI nr
Match: XP_004141603.2 (proline dehydrogenase 2, mitochondrial [Cucumis sativus] >KGN52551.1 hypothetical protein Csa_008665 [Cucumis sativus])

HSP 1 Score: 958 bits (2476), Expect = 0.0
Identity = 492/492 (100.00%), Postives = 492/492 (100.00%), Query Frame = 0

Query: 1   MAQAIVSTQSKISKRVFFYVFNRPLNTAASSPAAAFTAATATTSPPSPLHLNPPSTPTVD 60
           MAQAIVSTQSKISKRVFFYVFNRPLNTAASSPAAAFTAATATTSPPSPLHLNPPSTPTVD
Sbjct: 1   MAQAIVSTQSKISKRVFFYVFNRPLNTAASSPAAAFTAATATTSPPSPLHLNPPSTPTVD 60

Query: 61  FTDSRALFGSIPTSDLLHATATLHAAAVGPVVDVGMWVMNSKLMDVELFRDVVLGTVKHS 120
           FTDSRALFGSIPTSDLLHATATLHAAAVGPVVDVGMWVMNSKLMDVELFRDVVLGTVKHS
Sbjct: 61  FTDSRALFGSIPTSDLLHATATLHAAAVGPVVDVGMWVMNSKLMDVELFRDVVLGTVKHS 120

Query: 121 FYRHFCAGEDDTSVAKTVRRLHDVGLRSMLDYALEYADDEASCDRNLDGFLSTVEATKSL 180
           FYRHFCAGEDDTSVAKTVRRLHDVGLRSMLDYALEYADDEASCDRNLDGFLSTVEATKSL
Sbjct: 121 FYRHFCAGEDDTSVAKTVRRLHDVGLRSMLDYALEYADDEASCDRNLDGFLSTVEATKSL 180

Query: 181 PSGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLE 240
           PSGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLE
Sbjct: 181 PSGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLE 240

Query: 241 QPEPLTREEEKSLQMSHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNK 300
           QPEPLTREEEKSLQMSHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNK
Sbjct: 241 QPEPLTREEEKSLQMSHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNK 300

Query: 301 DRNPIVYGTIQAYLKDAKDRLLLANKEASKLKVPLGIKLVRGAYMSSESKLASSLGFESP 360
           DRNPIVYGTIQAYLKDAKDRLLLANKEASKLKVPLGIKLVRGAYMSSESKLASSLGFESP
Sbjct: 301 DRNPIVYGTIQAYLKDAKDRLLLANKEASKLKVPLGIKLVRGAYMSSESKLASSLGFESP 360

Query: 361 IHDTIQDTHSCYNTCASFLLDDIAKGSTGAILATHNVESGKLAASRAYEIGIGKLKQKLE 420
           IHDTIQDTHSCYNTCASFLLDDIAKGSTGAILATHNVESGKLAASRAYEIGIGKLKQKLE
Sbjct: 361 IHDTIQDTHSCYNTCASFLLDDIAKGSTGAILATHNVESGKLAASRAYEIGIGKLKQKLE 420

Query: 421 FAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRELMR 480
           FAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRELMR
Sbjct: 421 FAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRELMR 480

Query: 481 KELGRRMKEYIS 492
           KELGRRMKEYIS
Sbjct: 481 KELGRRMKEYIS 492

BLAST of CsGy5G029220 vs. NCBI nr
Match: KAA0039400.1 (proline dehydrogenase 2 [Cucumis melo var. makuwa] >TYK00588.1 proline dehydrogenase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 922 bits (2383), Expect = 0.0
Identity = 470/492 (95.53%), Postives = 482/492 (97.97%), Query Frame = 0

Query: 1   MAQAIVSTQSKISKRVFFYVFNRPLNTAASSPAAAFTAATATTSPPSPLHLNPPSTPTVD 60
           MA+A+VSTQSKISKRVF YVFNRPLNTAASSPAAAFTA      PP PLHLNPPSTPTVD
Sbjct: 1   MARALVSTQSKISKRVFLYVFNRPLNTAASSPAAAFTA------PPPPLHLNPPSTPTVD 60

Query: 61  FTDSRALFGSIPTSDLLHATATLHAAAVGPVVDVGMWVMNSKLMDVELFRDVVLGTVKHS 120
           FTDSRALFGSIPTSDLLHATATLHA+A+GPVVDVGMWVMNSKLMDVELFRDVVLGTVKH+
Sbjct: 61  FTDSRALFGSIPTSDLLHATATLHASAIGPVVDVGMWVMNSKLMDVELFRDVVLGTVKHT 120

Query: 121 FYRHFCAGEDDTSVAKTVRRLHDVGLRSMLDYALEYADDEASCDRNLDGFLSTVEATKSL 180
           FYRHFCAGEDDTSVAKTVRRLHDVGLRSMLDYALEYADDEASCDRNLDGFL TVEATKSL
Sbjct: 121 FYRHFCAGEDDTSVAKTVRRLHDVGLRSMLDYALEYADDEASCDRNLDGFLRTVEATKSL 180

Query: 181 PSGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLE 240
           PSGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLE
Sbjct: 181 PSGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLE 240

Query: 241 QPEPLTREEEKSLQMSHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNK 300
           QPEPLTREEEKSLQ+SHERLMKICQSCVD NVPLAIDAEHTKVQPAIDYFTYSAAIIHNK
Sbjct: 241 QPEPLTREEEKSLQISHERLMKICQSCVDTNVPLAIDAEHTKVQPAIDYFTYSAAIIHNK 300

Query: 301 DRNPIVYGTIQAYLKDAKDRLLLANKEASKLKVPLGIKLVRGAYMSSESKLASSLGFESP 360
           DRNPIVYGTIQAYLKDAKDRLLLANKEASKLK+PLGIKLVRGAYMSSESKLASSLGFESP
Sbjct: 301 DRNPIVYGTIQAYLKDAKDRLLLANKEASKLKIPLGIKLVRGAYMSSESKLASSLGFESP 360

Query: 361 IHDTIQDTHSCYNTCASFLLDDIAKGSTGAILATHNVESGKLAASRAYEIGIGKLKQKLE 420
           IH+TIQDTHSCYNTCASFLLDDIAKGS+GAILATHNVESGKLAA+RAYEIGIGKLKQKLE
Sbjct: 361 IHNTIQDTHSCYNTCASFLLDDIAKGSSGAILATHNVESGKLAATRAYEIGIGKLKQKLE 420

Query: 421 FAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRELMR 480
           FAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDR+LMR
Sbjct: 421 FAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRQLMR 480

Query: 481 KELGRRMKEYIS 492
           KELGRRMKEY+S
Sbjct: 481 KELGRRMKEYLS 486

BLAST of CsGy5G029220 vs. NCBI nr
Match: XP_008459446.1 (PREDICTED: proline dehydrogenase 2, mitochondrial [Cucumis melo])

HSP 1 Score: 909 bits (2348), Expect = 0.0
Identity = 463/491 (94.30%), Postives = 478/491 (97.35%), Query Frame = 0

Query: 1   MAQAIVSTQSKISKRVFFYVFNRPLNTAASSPAAAFTAATATTSPPSPLHLNPPSTPTVD 60
           MA+A+VSTQSKISKRVF YVFNRPLNTAASSPAAAFTA      PP PLHLNPPSTPTVD
Sbjct: 1   MARALVSTQSKISKRVFLYVFNRPLNTAASSPAAAFTA------PPPPLHLNPPSTPTVD 60

Query: 61  FTDSRALFGSIPTSDLLHATATLHAAAVGPVVDVGMWVMNSKLMDVELFRDVVLGTVKHS 120
           FTDSRALFGSIPTSDLLHATATLHA+A+GPVVDVGMWVMNSKLMDVELFRDVVLGTVKH+
Sbjct: 61  FTDSRALFGSIPTSDLLHATATLHASAIGPVVDVGMWVMNSKLMDVELFRDVVLGTVKHT 120

Query: 121 FYRHFCAGEDDTSVAKTVRRLHDVGLRSMLDYALEYADDEASCDRNLDGFLSTVEATKSL 180
           FYRHFCAGEDDTSVAKTVRRLHDVGLRSMLDYALEYADDEASCDRNLDGFL TVEATKSL
Sbjct: 121 FYRHFCAGEDDTSVAKTVRRLHDVGLRSMLDYALEYADDEASCDRNLDGFLRTVEATKSL 180

Query: 181 PSGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLE 240
           PSGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLE
Sbjct: 181 PSGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLE 240

Query: 241 QPEPLTREEEKSLQMSHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNK 300
           QPEPLTREEEKSLQ+SHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNK
Sbjct: 241 QPEPLTREEEKSLQISHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNK 300

Query: 301 DRNPIVYGTIQAYLKDAKDRLLLANKEASKLKVPLGIKLVRGAYMSSESKLASSLGFESP 360
           DRNPIVYGTIQAYLKDAKDRLLLANKEASKLK+PLGIKLVRGAYMSSESKLASSLGFESP
Sbjct: 301 DRNPIVYGTIQAYLKDAKDRLLLANKEASKLKIPLGIKLVRGAYMSSESKLASSLGFESP 360

Query: 361 IHDTIQDTHSCYNTCASFLLDDIAKGSTGAILATHNVESGKLAASRAYEIGIGKLKQKLE 420
           IH+TIQDTHSCYNTCASFLLDDIAKGS+GAILATHNVESGKLAA+RAYEIGIGKLKQKLE
Sbjct: 361 IHNTIQDTHSCYNTCASFLLDDIAKGSSGAILATHNVESGKLAATRAYEIGIGKLKQKLE 420

Query: 421 FAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRELMR 480
           FAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDR+LMR
Sbjct: 421 FAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRQLMR 480

Query: 481 KELGRRMKEYI 491
           KE G++ +  +
Sbjct: 481 KEFGKKDERVL 485

BLAST of CsGy5G029220 vs. NCBI nr
Match: XP_038891036.1 (proline dehydrogenase 2, mitochondrial-like [Benincasa hispida])

HSP 1 Score: 870 bits (2247), Expect = 0.0
Identity = 444/491 (90.43%), Postives = 466/491 (94.91%), Query Frame = 0

Query: 1   MAQAIVSTQSKISKRVFFYVFNRPLNTAASSPAAAFTAATATTSPPSPLHLNPPSTPTVD 60
           MA+ IVSTQSKI K+VFFYVFNR LNTAASS AAAFTAA   TSPP PL+LN P+TPTVD
Sbjct: 1   MARTIVSTQSKIPKKVFFYVFNRSLNTAASSSAAAFTAAA--TSPPLPLNLNSPTTPTVD 60

Query: 61  FTDSRALFGSIPTSDLLHATATLHAAAVGPVVDVGMWVMNSKLMDVELFRDVVLGTVKHS 120
           FTD+RALFGS+PTSDLLHA  TLHAAA+GPVVDVGMWVMNSKLMDVEL RD+VLGTVKH+
Sbjct: 61  FTDTRALFGSLPTSDLLHAATTLHAAAIGPVVDVGMWVMNSKLMDVELVRDLVLGTVKHT 120

Query: 121 FYRHFCAGEDDTSVAKTVRRLHDVGLRSMLDYALEYADDEASCDRNLDGFLSTVEATKSL 180
           FYR FCAGEDD +VAKTVRRLHDVGLRSMLDYALEYADDEASCDRNLDGFL T+E TKSL
Sbjct: 121 FYRQFCAGEDDATVAKTVRRLHDVGLRSMLDYALEYADDEASCDRNLDGFLRTIEITKSL 180

Query: 181 PSGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLE 240
           P GSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKL+TLPIFSESSPLYHTLE
Sbjct: 181 PPGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLKTLPIFSESSPLYHTLE 240

Query: 241 QPEPLTREEEKSLQMSHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNK 300
           QP+PLT EEEKSLQMSHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNK
Sbjct: 241 QPKPLTHEEEKSLQMSHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNK 300

Query: 301 DRNPIVYGTIQAYLKDAKDRLLLANKEASKLKVPLGIKLVRGAYMSSESKLASSLGFESP 360
           D  PIVYGTIQAYLKDAKDRLLL NKEASKLKVPLGIKLVRGAYMSSESK+ASSLGFESP
Sbjct: 301 DHIPIVYGTIQAYLKDAKDRLLLVNKEASKLKVPLGIKLVRGAYMSSESKIASSLGFESP 360

Query: 361 IHDTIQDTHSCYNTCASFLLDDIAKGSTGAILATHNVESGKLAASRAYEIGIGKLKQKLE 420
           IH+TIQDTH CYNTCASFLLD+IAKGS+G ILATHNVESGKLAA++AYEIG+GKLKQKLE
Sbjct: 361 IHNTIQDTHVCYNTCASFLLDNIAKGSSGVILATHNVESGKLAATKAYEIGMGKLKQKLE 420

Query: 421 FAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRELMR 480
           FAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLS SNLDR+LMR
Sbjct: 421 FAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSTSNLDRQLMR 480

Query: 481 KELGRRMKEYI 491
           KELGRRMKE +
Sbjct: 481 KELGRRMKECL 489

BLAST of CsGy5G029220 vs. NCBI nr
Match: XP_023551146.1 (proline dehydrogenase 2, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 809 bits (2089), Expect = 2.10e-292
Identity = 409/492 (83.13%), Postives = 454/492 (92.28%), Query Frame = 0

Query: 1   MAQAIVSTQSKISKRVFFYVFNRPLNTAASSPAAAFTAATATTSPPSPLHLN-PPSTPTV 60
           MA++I+STQSKI K VFFY FNR LNTAASS AAAFT+A A+T PP PL+L   P+ PTV
Sbjct: 1   MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSA-ASTPPPPPLNLAVSPAAPTV 60

Query: 61  DFTDSRALFGSIPTSDLLHATATLHAAAVGPVVDVGMWVMNSKLMDVELFRDVVLGTVKH 120
           DF+D++ALFGS+ T ++LHA A+LH AAVGPVVDVGMWVMNSKLMDVEL RDV+ GTV+H
Sbjct: 61  DFSDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTVRH 120

Query: 121 SFYRHFCAGEDDTSVAKTVRRLHDVGLRSMLDYALEYADDEASCDRNLDGFLSTVEATKS 180
           SFYRHFCAGEDDT+V KTVRRLHDVGLRSMLDYA+EYA+DE SCDRNL GFL T+EAT++
Sbjct: 121 SFYRHFCAGEDDTAVGKTVRRLHDVGLRSMLDYAIEYANDEESCDRNLHGFLRTIEATRA 180

Query: 181 LPSGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTL 240
           LP GSASFVV KVSAICPLRLLERVSDLLRWQQK+P  NLPWKL+T PIFSESSPLYHTL
Sbjct: 181 LPPGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPIFSESSPLYHTL 240

Query: 241 EQPEPLTREEEKSLQMSHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHN 300
           +QP PLT EE+KSLQ+SH+RLMKICQSCVDANVPLAIDAEHTK+QPAIDYFTYSAAIIHN
Sbjct: 241 QQPNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHN 300

Query: 301 KDRNPIVYGTIQAYLKDAKDRLLLANKEASKLKVPLGIKLVRGAYMSSESKLASSLGFES 360
           KD NPIVYGT+QAYLKDAKDRLLL  KEA+K++VPLGIKLVRGAYMSSESKLASSLGFES
Sbjct: 301 KDHNPIVYGTLQAYLKDAKDRLLLVTKEANKMRVPLGIKLVRGAYMSSESKLASSLGFES 360

Query: 361 PIHDTIQDTHSCYNTCASFLLDDIAKGSTGAILATHNVESGKLAASRAYEIGIGKLKQKL 420
           PIH++I DTH+CYN+CASFLLD+IA GS+GAILATHNV+SGKLAA +AYEIGI KLK+KL
Sbjct: 361 PIHNSIHDTHACYNSCASFLLDNIAVGSSGAILATHNVDSGKLAAKKAYEIGIEKLKEKL 420

Query: 421 EFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRELM 480
           EFAQLYGMSEALS+GLRNAGFQVSKYMPFG VDMVMPYLLRRAEENRGLLSASNLDR+LM
Sbjct: 421 EFAQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQLM 480

Query: 481 RKELGRRMKEYI 491
           RKEL RRMKEY+
Sbjct: 481 RKELRRRMKEYL 491

BLAST of CsGy5G029220 vs. ExPASy TrEMBL
Match: A0A0A0KXN4 (Proline dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_5G643300 PE=3 SV=1)

HSP 1 Score: 958 bits (2476), Expect = 0.0
Identity = 492/492 (100.00%), Postives = 492/492 (100.00%), Query Frame = 0

Query: 1   MAQAIVSTQSKISKRVFFYVFNRPLNTAASSPAAAFTAATATTSPPSPLHLNPPSTPTVD 60
           MAQAIVSTQSKISKRVFFYVFNRPLNTAASSPAAAFTAATATTSPPSPLHLNPPSTPTVD
Sbjct: 1   MAQAIVSTQSKISKRVFFYVFNRPLNTAASSPAAAFTAATATTSPPSPLHLNPPSTPTVD 60

Query: 61  FTDSRALFGSIPTSDLLHATATLHAAAVGPVVDVGMWVMNSKLMDVELFRDVVLGTVKHS 120
           FTDSRALFGSIPTSDLLHATATLHAAAVGPVVDVGMWVMNSKLMDVELFRDVVLGTVKHS
Sbjct: 61  FTDSRALFGSIPTSDLLHATATLHAAAVGPVVDVGMWVMNSKLMDVELFRDVVLGTVKHS 120

Query: 121 FYRHFCAGEDDTSVAKTVRRLHDVGLRSMLDYALEYADDEASCDRNLDGFLSTVEATKSL 180
           FYRHFCAGEDDTSVAKTVRRLHDVGLRSMLDYALEYADDEASCDRNLDGFLSTVEATKSL
Sbjct: 121 FYRHFCAGEDDTSVAKTVRRLHDVGLRSMLDYALEYADDEASCDRNLDGFLSTVEATKSL 180

Query: 181 PSGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLE 240
           PSGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLE
Sbjct: 181 PSGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLE 240

Query: 241 QPEPLTREEEKSLQMSHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNK 300
           QPEPLTREEEKSLQMSHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNK
Sbjct: 241 QPEPLTREEEKSLQMSHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNK 300

Query: 301 DRNPIVYGTIQAYLKDAKDRLLLANKEASKLKVPLGIKLVRGAYMSSESKLASSLGFESP 360
           DRNPIVYGTIQAYLKDAKDRLLLANKEASKLKVPLGIKLVRGAYMSSESKLASSLGFESP
Sbjct: 301 DRNPIVYGTIQAYLKDAKDRLLLANKEASKLKVPLGIKLVRGAYMSSESKLASSLGFESP 360

Query: 361 IHDTIQDTHSCYNTCASFLLDDIAKGSTGAILATHNVESGKLAASRAYEIGIGKLKQKLE 420
           IHDTIQDTHSCYNTCASFLLDDIAKGSTGAILATHNVESGKLAASRAYEIGIGKLKQKLE
Sbjct: 361 IHDTIQDTHSCYNTCASFLLDDIAKGSTGAILATHNVESGKLAASRAYEIGIGKLKQKLE 420

Query: 421 FAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRELMR 480
           FAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRELMR
Sbjct: 421 FAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRELMR 480

Query: 481 KELGRRMKEYIS 492
           KELGRRMKEYIS
Sbjct: 481 KELGRRMKEYIS 492

BLAST of CsGy5G029220 vs. ExPASy TrEMBL
Match: A0A5D3BNA9 (Proline dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G002050 PE=3 SV=1)

HSP 1 Score: 922 bits (2383), Expect = 0.0
Identity = 470/492 (95.53%), Postives = 482/492 (97.97%), Query Frame = 0

Query: 1   MAQAIVSTQSKISKRVFFYVFNRPLNTAASSPAAAFTAATATTSPPSPLHLNPPSTPTVD 60
           MA+A+VSTQSKISKRVF YVFNRPLNTAASSPAAAFTA      PP PLHLNPPSTPTVD
Sbjct: 1   MARALVSTQSKISKRVFLYVFNRPLNTAASSPAAAFTA------PPPPLHLNPPSTPTVD 60

Query: 61  FTDSRALFGSIPTSDLLHATATLHAAAVGPVVDVGMWVMNSKLMDVELFRDVVLGTVKHS 120
           FTDSRALFGSIPTSDLLHATATLHA+A+GPVVDVGMWVMNSKLMDVELFRDVVLGTVKH+
Sbjct: 61  FTDSRALFGSIPTSDLLHATATLHASAIGPVVDVGMWVMNSKLMDVELFRDVVLGTVKHT 120

Query: 121 FYRHFCAGEDDTSVAKTVRRLHDVGLRSMLDYALEYADDEASCDRNLDGFLSTVEATKSL 180
           FYRHFCAGEDDTSVAKTVRRLHDVGLRSMLDYALEYADDEASCDRNLDGFL TVEATKSL
Sbjct: 121 FYRHFCAGEDDTSVAKTVRRLHDVGLRSMLDYALEYADDEASCDRNLDGFLRTVEATKSL 180

Query: 181 PSGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLE 240
           PSGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLE
Sbjct: 181 PSGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLE 240

Query: 241 QPEPLTREEEKSLQMSHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNK 300
           QPEPLTREEEKSLQ+SHERLMKICQSCVD NVPLAIDAEHTKVQPAIDYFTYSAAIIHNK
Sbjct: 241 QPEPLTREEEKSLQISHERLMKICQSCVDTNVPLAIDAEHTKVQPAIDYFTYSAAIIHNK 300

Query: 301 DRNPIVYGTIQAYLKDAKDRLLLANKEASKLKVPLGIKLVRGAYMSSESKLASSLGFESP 360
           DRNPIVYGTIQAYLKDAKDRLLLANKEASKLK+PLGIKLVRGAYMSSESKLASSLGFESP
Sbjct: 301 DRNPIVYGTIQAYLKDAKDRLLLANKEASKLKIPLGIKLVRGAYMSSESKLASSLGFESP 360

Query: 361 IHDTIQDTHSCYNTCASFLLDDIAKGSTGAILATHNVESGKLAASRAYEIGIGKLKQKLE 420
           IH+TIQDTHSCYNTCASFLLDDIAKGS+GAILATHNVESGKLAA+RAYEIGIGKLKQKLE
Sbjct: 361 IHNTIQDTHSCYNTCASFLLDDIAKGSSGAILATHNVESGKLAATRAYEIGIGKLKQKLE 420

Query: 421 FAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRELMR 480
           FAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDR+LMR
Sbjct: 421 FAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRQLMR 480

Query: 481 KELGRRMKEYIS 492
           KELGRRMKEY+S
Sbjct: 481 KELGRRMKEYLS 486

BLAST of CsGy5G029220 vs. ExPASy TrEMBL
Match: A0A1S3CBE9 (Proline dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103498577 PE=3 SV=1)

HSP 1 Score: 909 bits (2348), Expect = 0.0
Identity = 463/491 (94.30%), Postives = 478/491 (97.35%), Query Frame = 0

Query: 1   MAQAIVSTQSKISKRVFFYVFNRPLNTAASSPAAAFTAATATTSPPSPLHLNPPSTPTVD 60
           MA+A+VSTQSKISKRVF YVFNRPLNTAASSPAAAFTA      PP PLHLNPPSTPTVD
Sbjct: 1   MARALVSTQSKISKRVFLYVFNRPLNTAASSPAAAFTA------PPPPLHLNPPSTPTVD 60

Query: 61  FTDSRALFGSIPTSDLLHATATLHAAAVGPVVDVGMWVMNSKLMDVELFRDVVLGTVKHS 120
           FTDSRALFGSIPTSDLLHATATLHA+A+GPVVDVGMWVMNSKLMDVELFRDVVLGTVKH+
Sbjct: 61  FTDSRALFGSIPTSDLLHATATLHASAIGPVVDVGMWVMNSKLMDVELFRDVVLGTVKHT 120

Query: 121 FYRHFCAGEDDTSVAKTVRRLHDVGLRSMLDYALEYADDEASCDRNLDGFLSTVEATKSL 180
           FYRHFCAGEDDTSVAKTVRRLHDVGLRSMLDYALEYADDEASCDRNLDGFL TVEATKSL
Sbjct: 121 FYRHFCAGEDDTSVAKTVRRLHDVGLRSMLDYALEYADDEASCDRNLDGFLRTVEATKSL 180

Query: 181 PSGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLE 240
           PSGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLE
Sbjct: 181 PSGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLE 240

Query: 241 QPEPLTREEEKSLQMSHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNK 300
           QPEPLTREEEKSLQ+SHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNK
Sbjct: 241 QPEPLTREEEKSLQISHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNK 300

Query: 301 DRNPIVYGTIQAYLKDAKDRLLLANKEASKLKVPLGIKLVRGAYMSSESKLASSLGFESP 360
           DRNPIVYGTIQAYLKDAKDRLLLANKEASKLK+PLGIKLVRGAYMSSESKLASSLGFESP
Sbjct: 301 DRNPIVYGTIQAYLKDAKDRLLLANKEASKLKIPLGIKLVRGAYMSSESKLASSLGFESP 360

Query: 361 IHDTIQDTHSCYNTCASFLLDDIAKGSTGAILATHNVESGKLAASRAYEIGIGKLKQKLE 420
           IH+TIQDTHSCYNTCASFLLDDIAKGS+GAILATHNVESGKLAA+RAYEIGIGKLKQKLE
Sbjct: 361 IHNTIQDTHSCYNTCASFLLDDIAKGSSGAILATHNVESGKLAATRAYEIGIGKLKQKLE 420

Query: 421 FAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRELMR 480
           FAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDR+LMR
Sbjct: 421 FAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRQLMR 480

Query: 481 KELGRRMKEYI 491
           KE G++ +  +
Sbjct: 481 KEFGKKDERVL 485

BLAST of CsGy5G029220 vs. ExPASy TrEMBL
Match: A0A6J1FGN7 (Proline dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111445322 PE=3 SV=1)

HSP 1 Score: 803 bits (2075), Expect = 1.32e-290
Identity = 408/494 (82.59%), Postives = 453/494 (91.70%), Query Frame = 0

Query: 1   MAQAIVSTQSKISKRVFFYVFNRPLNTAASSPAAAFTAATATTSPPSPLHLN---PPSTP 60
           MA++I+STQSKI K VFFY FNR LNTAASS AAAFT+A A+T PP   HLN    P+ P
Sbjct: 1   MARSILSTQSKIPKNVFFYAFNRLLNTAASSSAAAFTSA-ASTPPP---HLNLAVSPAAP 60

Query: 61  TVDFTDSRALFGSIPTSDLLHATATLHAAAVGPVVDVGMWVMNSKLMDVELFRDVVLGTV 120
           TVDF+D++ALFGS+ T ++LHA A+LH AAVGPVVDVGMWVMNSKLMDVEL RDV+ GTV
Sbjct: 61  TVDFSDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDVIFGTV 120

Query: 121 KHSFYRHFCAGEDDTSVAKTVRRLHDVGLRSMLDYALEYADDEASCDRNLDGFLSTVEAT 180
           +HSFYRHFCAGEDDT+V KTVRRLH+VGLRSMLDYA+EYA+DE SCDRNL GFL T+EAT
Sbjct: 121 RHSFYRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLHGFLRTIEAT 180

Query: 181 KSLPSGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYH 240
           + LP GSASFVV KVSAICPLRLLERVSDLLRWQQK+P  NLPWKL+T PIFSESSPLYH
Sbjct: 181 RVLPRGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPIFSESSPLYH 240

Query: 241 TLEQPEPLTREEEKSLQMSHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAII 300
           TL+QP PLT EE+KSLQ+SH+RLMKICQSCVDANVPLAIDAEHTK+QPAIDYFTYSAAII
Sbjct: 241 TLQQPNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAII 300

Query: 301 HNKDRNPIVYGTIQAYLKDAKDRLLLANKEASKLKVPLGIKLVRGAYMSSESKLASSLGF 360
           HNKD NPIVYGT+QAYLKDAKDRLLL  KEA++++VPLGIKLVRGAYMSSESKLASSLGF
Sbjct: 301 HNKDHNPIVYGTLQAYLKDAKDRLLLVTKEANEMRVPLGIKLVRGAYMSSESKLASSLGF 360

Query: 361 ESPIHDTIQDTHSCYNTCASFLLDDIAKGSTGAILATHNVESGKLAASRAYEIGIGKLKQ 420
           ESPIH++I DTH+CYN+CASFLLD+IA GS+GAILATHNVESGKLAA +AYEIGI KLK+
Sbjct: 361 ESPIHNSIHDTHACYNSCASFLLDNIATGSSGAILATHNVESGKLAAKKAYEIGIEKLKK 420

Query: 421 KLEFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRE 480
           KLEFAQLYGMSEALS+GLRNAGFQVSKYMPFG VDMVMPYLLRRAEENRGLLSASNLDR+
Sbjct: 421 KLEFAQLYGMSEALSYGLRNAGFQVSKYMPFGAVDMVMPYLLRRAEENRGLLSASNLDRQ 480

Query: 481 LMRKELGRRMKEYI 491
           LMRKELGRR+KEY+
Sbjct: 481 LMRKELGRRVKEYL 490

BLAST of CsGy5G029220 vs. ExPASy TrEMBL
Match: A0A6J1JZR8 (Proline dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111489341 PE=3 SV=1)

HSP 1 Score: 791 bits (2042), Expect = 1.12e-285
Identity = 401/492 (81.50%), Postives = 447/492 (90.85%), Query Frame = 0

Query: 1   MAQAIVSTQSKISKRVFFYVFNRPLNTAASSPAAAFTAATATTSPPSPLHLN-PPSTPTV 60
           MA++I+STQSKI K VFFY  NR LNTA     AAFT+A +T   P PL+L   P+ PTV
Sbjct: 1   MARSILSTQSKIPKNVFFYASNRLLNTA-----AAFTSAAST---PPPLNLAVSPAAPTV 60

Query: 61  DFTDSRALFGSIPTSDLLHATATLHAAAVGPVVDVGMWVMNSKLMDVELFRDVVLGTVKH 120
           DF+D++ALFGS+ T ++LHA A+LH AAVGPVVDVGMWVMNSKLMDVEL RD++ GTV+H
Sbjct: 61  DFSDTKALFGSLSTLEVLHAAASLHVAAVGPVVDVGMWVMNSKLMDVELARDIIFGTVRH 120

Query: 121 SFYRHFCAGEDDTSVAKTVRRLHDVGLRSMLDYALEYADDEASCDRNLDGFLSTVEATKS 180
           SFYRHFCAGEDDT+V KTVRRLH+VGLRSMLDYA+EYA+DE SCDRNL GFL T+EAT++
Sbjct: 121 SFYRHFCAGEDDTAVGKTVRRLHNVGLRSMLDYAIEYANDEESCDRNLRGFLRTIEATRA 180

Query: 181 LPSGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTL 240
           LP GSASFVV KVSAICPLRLLERVSDLLRWQQK+P  NLPWKL+T PIFSESSPLYHTL
Sbjct: 181 LPPGSASFVVAKVSAICPLRLLERVSDLLRWQQKDPCLNLPWKLKTFPIFSESSPLYHTL 240

Query: 241 EQPEPLTREEEKSLQMSHERLMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHN 300
           +QP PLT EE+KSLQ+SH+RLMKICQSCVDANVPLAIDAEHTK+QPAIDYFTYSAAIIHN
Sbjct: 241 QQPNPLTNEEQKSLQISHQRLMKICQSCVDANVPLAIDAEHTKIQPAIDYFTYSAAIIHN 300

Query: 301 KDRNPIVYGTIQAYLKDAKDRLLLANKEASKLKVPLGIKLVRGAYMSSESKLASSLGFES 360
           KD NPIVYGT+QAYLKDAKDRLLL  KEA+K++VPLGIKLVRGAYMSSESKLASSLGFES
Sbjct: 301 KDHNPIVYGTLQAYLKDAKDRLLLVTKEANKMRVPLGIKLVRGAYMSSESKLASSLGFES 360

Query: 361 PIHDTIQDTHSCYNTCASFLLDDIAKGSTGAILATHNVESGKLAASRAYEIGIGKLKQKL 420
           PIH++I DTH+CYN+CASFLLD+IA GS+GAILATHNVESGKL A +AYEIGI KLK+KL
Sbjct: 361 PIHNSIHDTHACYNSCASFLLDNIAAGSSGAILATHNVESGKLVAKKAYEIGIEKLKEKL 420

Query: 421 EFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRELM 480
           EFAQLYGMSEALS+GLRNAGFQVSKYMPFG VDMVMPYLLRRAEENRGLLSASNLDR+LM
Sbjct: 421 EFAQLYGMSEALSYGLRNAGFQVSKYMPFGGVDMVMPYLLRRAEENRGLLSASNLDRQLM 480

Query: 481 RKELGRRMKEYI 491
           RKELGRRMKEY+
Sbjct: 481 RKELGRRMKEYL 484

BLAST of CsGy5G029220 vs. TAIR 10
Match: AT3G30775.1 (Methylenetetrahydrofolate reductase family protein )

HSP 1 Score: 525.4 bits (1352), Expect = 4.9e-149
Identity = 269/470 (57.23%), Postives = 347/470 (73.83%), Query Frame = 0

Query: 24  PLNTAASSPAAAFTAATATTSPPSPLHLNPPSTPT---VDFTDSRALFGSIPTSDLLHAT 83
           P    AS+       +    +P  PLH   P+  +   +D +D   LF SIPTSDLL +T
Sbjct: 25  PPTVTASTAVVPEILSFGQQAPEPPLHHPKPTEQSHDGLDLSDQARLFSSIPTSDLLRST 84

Query: 84  ATLHAAAVGPVVDVGMWVMNSKLMDVELFRDVVLGTVKHSFYRHFCAGEDDTSVAKTVRR 143
           A LHAAA+GP+VD+G WVM+SKLMD  + R +VLG VK +FY HFCAGED  + A+ VR 
Sbjct: 85  AVLHAAAIGPMVDLGTWVMSSKLMDASVTRGMVLGLVKSTFYDHFCAGEDADAAAERVRS 144

Query: 144 LHD-VGLRSMLDYALEYADDEASCDRNLDGFLSTVEATKSLPSGSASFVVTKVSAICPLR 203
           +++  GL+ ML Y +E+ADD  SCD N+  F+ T+EA KSLP+   S VV K++AICP+ 
Sbjct: 145 VYEATGLKGMLVYGVEHADDAVSCDDNMQQFIRTIEAAKSLPTSHFSSVVVKITAICPIS 204

Query: 204 LLERVSDLLRWQQKNPSFNLPWKLQTLPIFSESSPLYHTLEQPEPLTREEEKSLQMSHER 263
           LL+RVSDLLRW+ K+P+F L WKL++ P+FSESSPLYHT  +PEPLT EEE+ L+ +H R
Sbjct: 205 LLKRVSDLLRWEYKSPNFKLSWKLKSFPVFSESSPLYHTNSEPEPLTAEEERELEAAHGR 264

Query: 264 LMKICQSCVDANVPLAIDAEHTKVQPAIDYFTYSAAIIHNKDRN-PIVYGTIQAYLKDAK 323
           + +IC+ C ++NVPL IDAE T +QPAIDY  YS+AI+ N D++ PIVY TIQAYL+DA 
Sbjct: 265 IQEICRKCQESNVPLLIDAEDTILQPAIDYMAYSSAIMFNADKDRPIVYNTIQAYLRDAG 324

Query: 324 DRLLLANKEASKLKVPLGIKLVRGAYMSSESKLASSLGFESPIHDTIQDTHSCYNTCASF 383
           +RL LA + A K  VP+G KLVRGAYMSSE+ LA SLG +SP+HDTIQDTHSCYN C +F
Sbjct: 325 ERLHLAVQNAEKENVPMGFKLVRGAYMSSEASLADSLGCKSPVHDTIQDTHSCYNDCMTF 384

Query: 384 LLDDIAKGS-TGAILATHNVESGKLAASRAYEIGIGKLKQKLEFAQLYGMSEALSFGLRN 443
           L++  + GS  G +LATHN +SG+LA+ +A ++GI K   K+EFAQLYGMS+ALSFGL+ 
Sbjct: 385 LMEKASNGSGFGVVLATHNADSGRLASRKASDLGIDKQNGKIEFAQLYGMSDALSFGLKR 444

Query: 444 AGFQVSKYMPFGPVDMVMPYLLRRAEENRGLLSASNLDRELMRKELGRRM 488
           AGF VSKYMPFGPV   +PYLLRRA ENRG+++    DR+LMR EL RR+
Sbjct: 445 AGFNVSKYMPFGPVATAIPYLLRRAYENRGMMATGAHDRQLMRMELKRRL 494

BLAST of CsGy5G029220 vs. TAIR 10
Match: AT5G38710.1 (Methylenetetrahydrofolate reductase family protein )

HSP 1 Score: 522.7 bits (1345), Expect = 3.2e-148
Identity = 263/450 (58.44%), Postives = 336/450 (74.67%), Query Frame = 0

Query: 42  TTSPPSPLHLNPPSTPT-VDFTDSRALFGSIPTSDLLHATATLHAAAVGPVVDVGMWVMN 101
           TT  P  L  + P     +D +D   LF S+P S LL +TA LHA ++GP+VD+G W+M+
Sbjct: 24  TTIIPEILSFDQPKPEVDLDLSDQARLFASVPISTLLRSTAILHATSIGPMVDLGSWLMS 83

Query: 102 SKLMDVELFRDVVLGTVKHSFYRHFCAGEDDTSVAKTVRRLHD-VGLRSMLDYALEYADD 161
           SKLMD  + RD+VL  VK +FY HFCAGED  + A+ V  +++  GL+ ML Y +E+A+D
Sbjct: 84  SKLMDTTVTRDLVLRIVKGTFYDHFCAGEDAAAAARRVSSVYESTGLKGMLVYGVEHAED 143

Query: 162 EASCDRNLDGFLSTVEATKSLPSGSASFVVTKVSAICPLRLLERVSDLLRWQQKNPSFNL 221
             +CD N+  F+ TVEA K+LPS   S VV K++AICP+ +L+RVSDLLRWQ KNP+F L
Sbjct: 144 GGACDENIQKFIETVEAAKTLPSSHLSSVVVKITAICPMNVLKRVSDLLRWQYKNPNFKL 203

Query: 222 PWKLQTLPIFSESSPLYHTLEQPEPLTREEEKSLQMSHERLMKICQSCVDANVPLAIDAE 281
           PWKL + P+FS  SPLYHT  +PEPLT EEE+ L+ +HERL  +C  C ++NVPL IDAE
Sbjct: 204 PWKLNSFPVFSGLSPLYHTTSEPEPLTVEEERELEKAHERLKSVCLRCQESNVPLLIDAE 263

Query: 282 HTKVQPAIDYFTYSAAIIHNKDRN-PIVYGTIQAYLKDAKDRLLLANKEASKLKVPLGIK 341
            T +QPAIDY  Y +AI+ N D++ PIVY TIQAYLKDA +RL LA +E+ K+ VP+G K
Sbjct: 264 DTILQPAIDYMAYWSAIMFNSDKDRPIVYNTIQAYLKDAGERLHLALRESEKMNVPIGFK 323

Query: 342 LVRGAYMSSESKLASSLGFESPIHDTIQDTHSCYNTCASFLLDDIAKGS-TGAILATHNV 401
           LVRGAYMSSE+KLA SLG++SP+HDTIQ+TH CYN C SFL++  + GS    ILATHN 
Sbjct: 324 LVRGAYMSSEAKLADSLGYKSPVHDTIQNTHDCYNDCMSFLMEKASNGSGIAVILATHNT 383

Query: 402 ESGKLAASRAYEIGIGKLKQKLEFAQLYGMSEALSFGLRNAGFQVSKYMPFGPVDMVMPY 461
           +SGKL A +A E+GI K   K+EFAQLYGMS+ALSFGL+ AGF VSKYMP+GPVD  +PY
Sbjct: 384 DSGKLGARKASELGINKENGKIEFAQLYGMSDALSFGLKRAGFNVSKYMPYGPVDTAIPY 443

Query: 462 LLRRAEENRGLLSASNLDRELMRKELGRRM 488
           L+RRA ENRG++S   LDR+LMRKEL RR+
Sbjct: 444 LIRRAYENRGMMSTGALDRQLMRKELKRRV 473

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P929836.9e-14857.23Proline dehydrogenase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=POX1 P... [more]
Q6NKX14.5e-14758.44Proline dehydrogenase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=POX2 P... [more]
Q86H287.9e-5133.18Proline dehydrogenase 1, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=p... [more]
O452282.8e-4025.50Proline dehydrogenase 1, mitochondrial OS=Caenorhabditis elegans OX=6239 GN=prdh... [more]
A6QQ741.5e-3831.35Hydroxyproline dehydrogenase OS=Bos taurus OX=9913 GN=PRODH2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_004141603.20.0100.00proline dehydrogenase 2, mitochondrial [Cucumis sativus] >KGN52551.1 hypothetica... [more]
KAA0039400.10.095.53proline dehydrogenase 2 [Cucumis melo var. makuwa] >TYK00588.1 proline dehydroge... [more]
XP_008459446.10.094.30PREDICTED: proline dehydrogenase 2, mitochondrial [Cucumis melo][more]
XP_038891036.10.090.43proline dehydrogenase 2, mitochondrial-like [Benincasa hispida][more]
XP_023551146.12.10e-29283.13proline dehydrogenase 2, mitochondrial-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A0A0KXN40.0100.00Proline dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_5G643300 PE=3 SV=1[more]
A0A5D3BNA90.095.53Proline dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16... [more]
A0A1S3CBE90.094.30Proline dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103498577 PE=3 SV=1[more]
A0A6J1FGN71.32e-29082.59Proline dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111445322 PE=3 SV=1[more]
A0A6J1JZR81.12e-28581.50Proline dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111489341 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G30775.14.9e-14957.23Methylenetetrahydrofolate reductase family protein [more]
AT5G38710.13.2e-14858.44Methylenetetrahydrofolate reductase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002872Proline dehydrogenase domainPFAMPF01619Pro_dhcoord: 135..470
e-value: 1.6E-72
score: 244.4
NoneNo IPR availableGENE3D3.20.20.220coord: 101..489
e-value: 1.1E-90
score: 306.5
NoneNo IPR availablePANTHERPTHR13914:SF25PROLINE DEHYDROGENASEcoord: 9..489
IPR015659Proline oxidase familyPANTHERPTHR13914PROLINE OXIDASEcoord: 9..489
IPR029041FAD-linked oxidoreductase-likeSUPERFAMILY51730FAD-linked oxidoreductasecoord: 128..490

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy5G029220.1CsGy5G029220.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010133 proline catabolic process to glutamate
biological_process GO:0006562 proline catabolic process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005739 mitochondrion
molecular_function GO:0071949 FAD binding
molecular_function GO:0004657 proline dehydrogenase activity