CsGy5G028530 (gene) Cucumber (Gy14) v2.1

Overview


Name	CsGy5G028530
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description	Pectinesterase
Location	Gy14Chr5: 32139651 .. 32142640 (+)
RNA-Seq Expression	CsGy5G028530
Synteny	CsGy5G028530

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGCCCAGCCCAGGGTCCTCACAGTAGCACACGACGGCTGTGCAGATTTCTGCACGGTTCAAGAAGCCATAGACGCGGTTCCATTCAGCAATACTTGCCGCACTATAATCCGTGTGTCTCCTGGAATTTACAAGCAGCCTTTATATGTACCCAAGACCAAAAATTTCATTACTTTCGCCGGTTTGAACCCTGAGACTACTATTCTCACTTGGGACAATACTGCCACCAAAATTAACCACCACCAAGTCCGTTTCTCATCTGTTTTCTTTTTTATCATTTCGTATCTGGGTTTTCTTTTAATTCTCAGAATTTGTAATCTTCTTTTCATATTTCCCTCTTTCGTGGTCAGGCTGCTCGTGTCATTGGAACTGGGACTTTTGGTTGTGGAAGTACAATTGTGGAAGGAGAGGATTTCCTTGCTGAGAATATCACGTTTGAGAATTCTTCCCCTCAAGTACCCTTTTACTTTTAAGACACCTTTGATCATCTTATCTTATCTTTACTTTGGATTTTGGATTTTGGATTTTGGATTTACTTTCTCACTCATTAAACATATCAGTAATATGGAGATGGAACTAATAATTAAAACCCATTTGTATTTCAATTTTGAGGTTGATTTTTGAAATTGTACCATGAGGATCTTTGTACAAGCTAATTTGCTGCGTTTTTCTGATCAACTTCCTTAGTGTTTTGAAGTTGGTGTTGTTTTAGTTATCTTGTGATTTCTTTGTTTTCTTCAGGGTTCTGGACAAGCTGTGGCTATCAGAGTTACAGCAGATCGTTGTGCCTTTTACAATTGCAGGTTTCTTGGTTGGCAGGTATTCCAATAGTGTATAGTTAGTTCAAGAAGTCTAACAATATTAAAGGCCTTTTTACCATAGAAACTTGTGCTTGGTTTGTTAGGGTGGATGTTTAACTTACCATATTTATTCAAAGAGGTATTTCCTAAGCTAATAAAGAGTGCGATGTTCTACGGTGCTGATCCTGGTCTTGGGCTAAGTTCGAGAGTAGGCCTGAATTTCCTCTCTTATCCATTATCAGTTATCTATTGTTGAGGATTCCACATTAGAATAATGAGGGACCATAACGTTTGCAAGATACATGAGCTACTCCTCTCCTGACTAGTTTTGAGAAGTAACCCCATGTTATGTAATATCTATATTGTCCAACTTTTAATGAAGTTGGCTCAATTCCATCAAGCTTTCTTTGCTTTTGCTATCTATAACTCGTGACTAGAATCATCAGTTTGATGCTGTTGTTGGCTACCTCAATCAGCCAATGCTGTTTGTGACGAAAGTAGATTGTTTTTTTGTACCTCTATTGCCTGCTATTGTTTCTTCATGTGAAAGACTTTTGGATGCCTAGGAAAAGCTTTGATCAGTTCCATTTTCCCTATATAATTTGATGCAGGATACTCTGTACCTACATTATGGAAGACAATATTTGAAGGATTGTTACATCGAAGGAAGCGTGGACTTCATATTTGGCAACAGCACTGCTCTGTTGGAGCATTGTCATGTCCACTGCAAGTCGAAAGGCTTTATTACTGCACAGAGCAGAAAATCTTCTCAAGAGACGACAGGTTATGTGTTCTTGAGGTGTGTTGGTGGAAACTATGTTCCTCTTGTTAGAAATGTCTGGTGTCTCATGGATTGTTGAAACAATGGTTCATGTTTATTAGGTTATTATAAAATAATAAAATATAAAGTGACAATGAGATTATGATTCAAACTCTATGCTGCCCGGCCAGGAAAAGAAATAGGATATTAAAATATGTTTTCTAACAATCAAATATTGTAGCATTAAATGGTTCTCGTGTGATATTAGTTGGAGGTGTACTTAACTAGTTAAGATGCTCATATTATATATAAAGGAAACTCTTTGAAAATACTGGATCTTCCAGTCTCTAATTAGTTTTTTGCTGTTATGAATCTGACTTTGAGATAAGATTTTGAAGCACTAACCATTAATTTACCCCAAAAACCAAGATATGCTTTTGTATGAGTTATTGCAATCTACTATATTGAATAGAGTTTCAAAGAAGGTCGATACTTTATCACAAGTGTTCTTTTAAAATGTTCACTGTTACATTCTGAAATGCTTTCTTGTGATAGCTTAAAATTTTGTCCGACTGTGCTAAATCTTCATCATATCCTTTTCTTTGGAAATGTTTTGACTCTCTTTTGATTAATGTAGGTGTGTGATCACAGGCAGTGGTGAGACTTCTTACGTTCATCTCGGGCGACCATGGGGACCATTTGCAAGGGTGGTATTTGCTTACACACACATGGATGTATGTATCAAACCAGCAGGCTGGGATAACTGGGGGAAAGCTGAAAACGAGAGAACTGCTTGCTTCTACGAGTACAAGTATGTTGTGTTTAATGCTTTTTTTCATCTTATCGACAATGACTATACGATGAATGAACATGAACATCATCGAGTGATCAAGCATTGATCACCTGATAGGAGACGTGTGTTTTCCGGTACAGGTGTTTCGGGCCAGGGAGTTGCTCAATGAAAAGGGTGTGTTGGGCAAGAGAATTATTGGATGAAGAAGCTGATGAGTTCATTCTGCATCGATTTATCGATCCCGACGTGGATAGGCCTTGGCTATGCCAGAGGATGGCACTGAGGATTCCTTTTTCTGCTTAA

mRNA sequence

ATGGCGGCCCAGCCCAGGGTCCTCACAGTAGCACACGACGGCTGTGCAGATTTCTGCACGGTTCAAGAAGCCATAGACGCGGTTCCATTCAGCAATACTTGCCGCACTATAATCCGTGTGTCTCCTGGAATTTACAAGCAGCCTTTATATGTACCCAAGACCAAAAATTTCATTACTTTCGCCGGTTTGAACCCTGAGACTACTATTCTCACTTGGGACAATACTGCCACCAAAATTAACCACCACCAAGCTGCTCGTGTCATTGGAACTGGGACTTTTGGTTGTGGAAGTACAATTGTGGAAGGAGAGGATTTCCTTGCTGAGAATATCACGTTTGAGAATTCTTCCCCTCAAGGTTCTGGACAAGCTGTGGCTATCAGAGTTACAGCAGATCGTTGTGCCTTTTACAATTGCAGGTTTCTTGGTTGGCAGGATACTCTGTACCTACATTATGGAAGACAATATTTGAAGGATTGTTACATCGAAGGAAGCGTGGACTTCATATTTGGCAACAGCACTGCTCTGTTGGAGCATTGTCATGTCCACTGCAAGTCGAAAGGCTTTATTACTGCACAGAGCAGAAAATCTTCTCAAGAGACGACAGGTTATGTGTTCTTGAGGTGTGTGATCACAGGCAGTGGTGAGACTTCTTACGTTCATCTCGGGCGACCATGGGGACCATTTGCAAGGGTGGTATTTGCTTACACACACATGGATGTATGTATCAAACCAGCAGGCTGGGATAACTGGGGGAAAGCTGAAAACGAGAGAACTGCTTGCTTCTACGAGTACAAGTGTTTCGGGCCAGGGAGTTGCTCAATGAAAAGGGTGTGTTGGGCAAGAGAATTATTGGATGAAGAAGCTGATGAGTTCATTCTGCATCGATTTATCGATCCCGACGTGGATAGGCCTTGGCTATGCCAGAGGATGGCACTGAGGATTCCTTTTTCTGCTTAA

Coding sequence (CDS)

ATGGCGGCCCAGCCCAGGGTCCTCACAGTAGCACACGACGGCTGTGCAGATTTCTGCACGGTTCAAGAAGCCATAGACGCGGTTCCATTCAGCAATACTTGCCGCACTATAATCCGTGTGTCTCCTGGAATTTACAAGCAGCCTTTATATGTACCCAAGACCAAAAATTTCATTACTTTCGCCGGTTTGAACCCTGAGACTACTATTCTCACTTGGGACAATACTGCCACCAAAATTAACCACCACCAAGCTGCTCGTGTCATTGGAACTGGGACTTTTGGTTGTGGAAGTACAATTGTGGAAGGAGAGGATTTCCTTGCTGAGAATATCACGTTTGAGAATTCTTCCCCTCAAGGTTCTGGACAAGCTGTGGCTATCAGAGTTACAGCAGATCGTTGTGCCTTTTACAATTGCAGGTTTCTTGGTTGGCAGGATACTCTGTACCTACATTATGGAAGACAATATTTGAAGGATTGTTACATCGAAGGAAGCGTGGACTTCATATTTGGCAACAGCACTGCTCTGTTGGAGCATTGTCATGTCCACTGCAAGTCGAAAGGCTTTATTACTGCACAGAGCAGAAAATCTTCTCAAGAGACGACAGGTTATGTGTTCTTGAGGTGTGTGATCACAGGCAGTGGTGAGACTTCTTACGTTCATCTCGGGCGACCATGGGGACCATTTGCAAGGGTGGTATTTGCTTACACACACATGGATGTATGTATCAAACCAGCAGGCTGGGATAACTGGGGGAAAGCTGAAAACGAGAGAACTGCTTGCTTCTACGAGTACAAGTGTTTCGGGCCAGGGAGTTGCTCAATGAAAAGGGTGTGTTGGGCAAGAGAATTATTGGATGAAGAAGCTGATGAGTTCATTCTGCATCGATTTATCGATCCCGACGTGGATAGGCCTTGGCTATGCCAGAGGATGGCACTGAGGATTCCTTTTTCTGCTTAA

Protein sequence

MAAQPRVLTVAHDGCADFCTVQEAIDAVPFSNTCRTIIRVSPGIYKQPLYVPKTKNFITFAGLNPETTILTWDNTATKINHHQAARVIGTGTFGCGSTIVEGEDFLAENITFENSSPQGSGQAVAIRVTADRCAFYNCRFLGWQDTLYLHYGRQYLKDCYIEGSVDFIFGNSTALLEHCHVHCKSKGFITAQSRKSSQETTGYVFLRCVITGSGETSYVHLGRPWGPFARVVFAYTHMDVCIKPAGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELLDEEADEFILHRFIDPDVDRPWLCQRMALRIPFSA*

Homology

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BLAST of CsGy5G028530 vs. ExPASy Swiss-Prot
Match: Q9LVQ0 (Pectinesterase 31 OS=Arabidopsis thaliana OX=3702 GN=PME31 PE=1 SV=1)

HSP 1 Score: 560.8 bits (1444), Expect = 9.6e-159
Identity = 242/316 (76.58%), Postives = 286/316 (90.51%), Query Frame = 0


Query: 3   AQPRVLTVAHDGCADFCTVQEAIDAVPFSNTCRTIIRVSPGIYKQPLYVPKTKNFITFAG 62
           A  R++ V+ DG  D+C+VQ+AID+VP  NTCRT+IR+SPGIY+QP+YVPK KNFITFAG
Sbjct: 2   ATTRMVRVSQDGSGDYCSVQDAIDSVPLGNTCRTVIRLSPGIYRQPVYVPKRKNFITFAG 61

Query: 63  LNPETTILTWDNTATKINHHQAARVIGTGTFGCGSTIVEGEDFLAENITFENSSPQGSGQ 122
           ++PE T+LTW+NTA+KI HHQA+RVIGTGTFGCGS IVEGEDF+AENITFENS+P+GSGQ
Sbjct: 62  ISPEITVLTWNNTASKIEHHQASRVIGTGTFGCGSVIVEGEDFIAENITFENSAPEGSGQ 121

Query: 123 AVAIRVTADRCAFYNCRFLGWQDTLYLHYGRQYLKDCYIEGSVDFIFGNSTALLEHCHVH 182
           AVAIRVTADRCAFYNCRFLGWQDTLYLH+G+QYLKDCYIEGSVDFIFGNSTALLEHCH+H
Sbjct: 122 AVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLEHCHIH 181

Query: 183 CKSKGFITAQSRKSSQETTGYVFLRCVITGSGETSYVHLGRPWGPFARVVFAYTHMDVCI 242
           CKS+GFITAQSRKSSQE+TGYVFLRCVITG+G++ Y++LGRPWGPF RVV AYT+MD CI
Sbjct: 182 CKSQGFITAQSRKSSQESTGYVFLRCVITGNGQSGYMYLGRPWGPFGRVVLAYTYMDACI 241

Query: 243 KPAGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELLDEEADEFILHRFIDPDVD 302
           +  GW NWG AENER+ACFYEY+CFGPGSCS +RV W+REL+D+EA  F+ H F+DP+ D
Sbjct: 242 RNVGWHNWGNAENERSACFYEYRCFGPGSCSSERVPWSRELMDDEAGHFVHHSFVDPEQD 301

Query: 303 RPWLCQRMALRIPFSA 319
           RPWLC RM ++ P+SA
Sbjct: 302 RPWLCLRMGVKTPYSA 317

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BLAST of CsGy5G028530 vs. ExPASy Swiss-Prot
Match: Q8VYZ3 (Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 6.9e-72
Identity = 140/289 (48.44%), Postives = 179/289 (61.94%), Query Frame = 0


Query: 17  DFCTVQEAIDAVPFSNTCRTIIRVSPGIYKQPLYVPKTKNFITFAGLNPETTILTWDNTA 76
           DF  +Q+AID++P  N  R +I+V  G+YK+ + +P  K FIT  G   E T + W +TA
Sbjct: 95  DFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVEWGDTA 154

Query: 77  TKINHHQAARVIGTGTFGCGSTIVEGEDFLAENITFENSSP-----QGSGQAVAIRVTAD 136
              +    ++    GT+   S  V    F+A+NITF N++P         QAVA+RV+AD
Sbjct: 155 QTPD----SKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSAD 214

Query: 137 RCAFYNCRFLGWQDTLYLHYGRQYLKDCYIEGSVDFIFGNSTALLEHCHVH--CKSKGFI 196
             AF+ CR LG QDTLY H GR Y KDCYIEGSVDFIFGN+ +L E CHVH      G +
Sbjct: 215 NAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGAV 274

Query: 197 TAQSRKSSQETTGYVFLRCVITGSGETSYVHLGRPWGPFARVVFAYTHMDVCIKPAGWDN 256
           TAQ R S  E TG+ F++C +TG+G    ++LGR WGPF+RVVFAYT+MD  I P GW N
Sbjct: 275 TAQGRSSVLEDTGFSFVKCKVTGTG---VLYLGRAWGPFSRVVFAYTYMDNIILPRGWYN 334

Query: 257 WGKAENERTACFYEYKCFGPGSCSMKRVCWARELLDEEADEFILHRFID 299
           WG    E T  + +YKC G G+    RV WAREL DEEA  F+   FID
Sbjct: 335 WGDPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLTFID 376

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BLAST of CsGy5G028530 vs. ExPASy Swiss-Prot
Match: Q9ZQA3 (Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 4.6e-60
Identity = 123/306 (40.20%), Postives = 177/306 (57.84%), Query Frame = 0


Query: 7   VLTVAHDGCADFCTVQEAIDAVPFSNTCRTIIRVSPGIYKQPLYVPKTKNFITFAGLNPE 66
           VLTV   GC +F  VQ AID VP  ++ +T+I V+ G Y++ + V + K  +   G   +
Sbjct: 90  VLTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENKTNLVIQGRGYQ 149

Query: 67  TTILTWDNTATKINHHQAARVIGTGTFGCGSTIVEGEDFLAENITFENSSP-----QGSG 126
            T + W++TA    +          T    S +V   +F A NI+F+N++P     +   
Sbjct: 150 NTSIEWNDTAKSAGN----------TADSFSFVVFAANFTAYNISFKNNAPEPDPGEADA 209

Query: 127 QAVAIRVTADRCAFYNCRFLGWQDTLYLHYGRQYLKDCYIEGSVDFIFGNSTALLEHCHV 186
           QAVA+R+  D+ AFY C F G QDTL    GR + K+C+I+GS+DFIFGN  +L + C +
Sbjct: 210 QAVALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRSLYQDCTI 269

Query: 187 HCKSK-------GFITAQSRKSSQETTGYVFLRCVITGSGETSYVHLGRPWGPFARVVFA 246
           +  +K       G ITAQ R+S  E +G+ F+ C I GSGE   + LGR WG +A VVF+
Sbjct: 270 NSIAKGNTSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGE---ILLGRAWGAYATVVFS 329

Query: 247 YTHMDVCIKPAGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELLDEEADEFILH 301
            T+M   I P GW+NWG +  E+T  F E+KC+GPG+   +RV + ++L D EA  FI  
Sbjct: 330 NTYMSGIITPEGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQLTDSEASSFIDV 382

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BLAST of CsGy5G028530 vs. ExPASy Swiss-Prot
Match: Q8LPF3 (Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 6.7e-59
Identity = 119/315 (37.78%), Postives = 180/315 (57.14%), Query Frame = 0


Query: 6   RVLTVAHDGCADFCTVQEAIDAVPFSNTCRTIIRVSPGIYKQPLYVPKTKNFITFAGLNP 65
           +V+TV+ +G A F +VQ+A+D++P +N     I+++PG Y++ + VP TK +ITF G   
Sbjct: 59  KVITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGR 118

Query: 66  ETTILTWDNTATKIN-HHQAARVIGTGTFGCGSTIVEGEDFLAENITFENSSP-----QG 125
           + T + W + A+ +  + Q  R     T+   S  V    F A NI+F N++P       
Sbjct: 119 DVTAIEWHDRASDLGANGQQLR-----TYQTASVTVYANYFTARNISFTNTAPAPLPGMQ 178

Query: 126 SGQAVAIRVTADRCAFYNCRFLGWQDTLYLHYGRQYLKDCYIEGSVDFIFGNSTALLEHC 185
             QAVA R++ D+  F  C F G QDTL    GR Y K+CYIEGS+DFIFGN  ++ + C
Sbjct: 179 GWQAVAFRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDC 238

Query: 186 HVHCKSK--GFITAQSRKSSQETTGYVFLRCVITGSGETSYVHLGRPWGPFARVVFAYTH 245
            +H  +   G I A  R   +E TG+ F+ C +TG+G    +++GR  G ++R+V+AYT+
Sbjct: 239 ELHSIASRFGSIAAHGRTCPEEKTGFAFVGCRVTGTGP---LYVGRAMGQYSRIVYAYTY 298

Query: 246 MDVCIKPAGWDNWG-KAENERTACFYEYKCFGPGSCSMKRVCWARELLDEEADEFILHRF 305
            D  +   GWD+W  K+   +TA F  Y C+GPG+ + + V WAR L  E A  FI   F
Sbjct: 299 FDALVAHGGWDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSF 358

Query: 306 IDPDVDRPWLCQRMA 312
           ++    R W+  R A
Sbjct: 359 VN---GRHWIAPRDA 362

[Close]

BLAST of CsGy5G028530 vs. ExPASy Swiss-Prot
Match: Q9SIJ9 (Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 1.9e-58
Identity = 117/295 (39.66%), Postives = 167/295 (56.61%), Query Frame = 0


Query: 7   VLTVAHDGCADFCTVQEAIDAVP--FSNTCRTIIRVSPGIYKQPLYVPKTKNFITFAGLN 66
           ++ V   G  DF  +QEAI+++P   +N+    I V PGIY++ + +P  K +IT +G  
Sbjct: 50  LIRVDQSGKGDFSKIQEAIESIPPNLNNSQLYFIWVKPGIYREKVVIPAEKPYITLSGTQ 109

Query: 67  PETTILTWDNTATKINHHQAARVIGTGTFGCGSTIVEGEDFLAENITFENSSPQGSGQAV 126
              T L W +              G       +  +   DF+   +T +N     +G+AV
Sbjct: 110 ASNTFLIWSD--------------GEDILESPTLTIFASDFVCRFLTIQNKFGT-AGRAV 169

Query: 127 AIRVTADRCAFYNCRFLGWQDTLYLHYGRQYLKDCYIEGSVDFIFGNSTALLEHCHVHCK 186
           A+RV AD+ AFY C    +QDTL    G  Y K+CYIEG+ DFI G++++L E CH+H  
Sbjct: 170 ALRVAADKAAFYGCVITSYQDTLLDDNGNHYFKNCYIEGATDFICGSASSLYERCHLHSL 229

Query: 187 S--KGFITAQSRKSSQETTGYVFLRCVITGSGETSYVHLGRPWGPFARVVFAYTHMDVCI 246
           S   G ITAQ R S+ E +G+ FL C +TGSG T    LGRPWG ++RVVFAY+     +
Sbjct: 230 SPNNGSITAQMRTSATEKSGFTFLGCKLTGSGST---FLGRPWGAYSRVVFAYSFFSNVV 289

Query: 247 KPAGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELLDEEADEFILHRFI 298
            P GW+ WG +  E T  + EYKC+GPG+   +RV W+++L DEEA  F+   FI
Sbjct: 290 APQGWNQWGDSTKENTVYYGEYKCYGPGADREQRVEWSKQLSDEEATVFLSKDFI 326

[Close]

BLAST of CsGy5G028530 vs. NCBI nr
Match: XP_004141450.1 (pectinesterase 31 [Cucumis sativus] >KGN52482.1 hypothetical protein Csa_007777 [Cucumis sativus])

HSP 1 Score: 680 bits (1754), Expect = 2.52e-247
Identity = 318/318 (100.00%), Postives = 318/318 (100.00%), Query Frame = 0


Query: 1   MAAQPRVLTVAHDGCADFCTVQEAIDAVPFSNTCRTIIRVSPGIYKQPLYVPKTKNFITF 60
           MAAQPRVLTVAHDGCADFCTVQEAIDAVPFSNTCRTIIRVSPGIYKQPLYVPKTKNFITF
Sbjct: 1   MAAQPRVLTVAHDGCADFCTVQEAIDAVPFSNTCRTIIRVSPGIYKQPLYVPKTKNFITF 60

Query: 61  AGLNPETTILTWDNTATKINHHQAARVIGTGTFGCGSTIVEGEDFLAENITFENSSPQGS 120
           AGLNPETTILTWDNTATKINHHQAARVIGTGTFGCGSTIVEGEDFLAENITFENSSPQGS
Sbjct: 61  AGLNPETTILTWDNTATKINHHQAARVIGTGTFGCGSTIVEGEDFLAENITFENSSPQGS 120

Query: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYGRQYLKDCYIEGSVDFIFGNSTALLEHCH 180
           GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYGRQYLKDCYIEGSVDFIFGNSTALLEHCH
Sbjct: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYGRQYLKDCYIEGSVDFIFGNSTALLEHCH 180

Query: 181 VHCKSKGFITAQSRKSSQETTGYVFLRCVITGSGETSYVHLGRPWGPFARVVFAYTHMDV 240
           VHCKSKGFITAQSRKSSQETTGYVFLRCVITGSGETSYVHLGRPWGPFARVVFAYTHMDV
Sbjct: 181 VHCKSKGFITAQSRKSSQETTGYVFLRCVITGSGETSYVHLGRPWGPFARVVFAYTHMDV 240

Query: 241 CIKPAGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELLDEEADEFILHRFIDPD 300
           CIKPAGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELLDEEADEFILHRFIDPD
Sbjct: 241 CIKPAGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELLDEEADEFILHRFIDPD 300

Query: 301 VDRPWLCQRMALRIPFSA 318
           VDRPWLCQRMALRIPFSA
Sbjct: 301 VDRPWLCQRMALRIPFSA 318

[Close]

BLAST of CsGy5G028530 vs. NCBI nr
Match: KAA0039456.1 (pectinesterase 31 [Cucumis melo var. makuwa] >TYK00646.1 pectinesterase 31 [Cucumis melo var. makuwa])

HSP 1 Score: 669 bits (1725), Expect = 6.67e-243
Identity = 312/318 (98.11%), Postives = 315/318 (99.06%), Query Frame = 0


Query: 1   MAAQPRVLTVAHDGCADFCTVQEAIDAVPFSNTCRTIIRVSPGIYKQPLYVPKTKNFITF 60
           MAAQPRVLTVAHDGCADF TVQEAIDAVPFSNTCRT+IRVSPGIYKQPLYVPKTKNFITF
Sbjct: 1   MAAQPRVLTVAHDGCADFRTVQEAIDAVPFSNTCRTVIRVSPGIYKQPLYVPKTKNFITF 60

Query: 61  AGLNPETTILTWDNTATKINHHQAARVIGTGTFGCGSTIVEGEDFLAENITFENSSPQGS 120
           AGLNPETTILTWDNTAT+I HHQAARVIGTGTFGCGSTIVEGEDF+AENITFENSSPQGS
Sbjct: 61  AGLNPETTILTWDNTATEIKHHQAARVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120

Query: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYGRQYLKDCYIEGSVDFIFGNSTALLEHCH 180
           GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYGRQYLKDCYIEGSVDFIFGNSTALLEHCH
Sbjct: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYGRQYLKDCYIEGSVDFIFGNSTALLEHCH 180

Query: 181 VHCKSKGFITAQSRKSSQETTGYVFLRCVITGSGETSYVHLGRPWGPFARVVFAYTHMDV 240
           VHCKSKGFITAQSRKSSQETTGYVFLRCVITGSGETSYVHLGRPWGPFARVVFAYTHMDV
Sbjct: 181 VHCKSKGFITAQSRKSSQETTGYVFLRCVITGSGETSYVHLGRPWGPFARVVFAYTHMDV 240

Query: 241 CIKPAGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELLDEEADEFILHRFIDPD 300
           CIKP GWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELLDEEADEFILHRFIDPD
Sbjct: 241 CIKPTGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELLDEEADEFILHRFIDPD 300

Query: 301 VDRPWLCQRMALRIPFSA 318
           VDRPWLCQRMALRIPFSA
Sbjct: 301 VDRPWLCQRMALRIPFSA 318

[Close]

BLAST of CsGy5G028530 vs. NCBI nr
Match: XP_038891224.1 (pectinesterase 31 [Benincasa hispida])

HSP 1 Score: 644 bits (1662), Expect = 2.69e-233
Identity = 296/318 (93.08%), Postives = 312/318 (98.11%), Query Frame = 0


Query: 1   MAAQPRVLTVAHDGCADFCTVQEAIDAVPFSNTCRTIIRVSPGIYKQPLYVPKTKNFITF 60
           MAA PR+LTVAHDGCADF TVQEAIDAVPFSNTCRTIIRVSPGIY+QPLYVPKTKNFITF
Sbjct: 1   MAAPPRLLTVAHDGCADFRTVQEAIDAVPFSNTCRTIIRVSPGIYRQPLYVPKTKNFITF 60

Query: 61  AGLNPETTILTWDNTATKINHHQAARVIGTGTFGCGSTIVEGEDFLAENITFENSSPQGS 120
           AGLNPETTILTWDNTATKI+HHQA+RVIGTGTFGCG+TIVEGEDF+AENITFENSSPQGS
Sbjct: 61  AGLNPETTILTWDNTATKISHHQASRVIGTGTFGCGTTIVEGEDFIAENITFENSSPQGS 120

Query: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYGRQYLKDCYIEGSVDFIFGNSTALLEHCH 180
           GQAVAIRVTADRCAFYNCRFLGWQDTLYLH+GRQYLKDCYIEGSVDFIFGNSTALLEHCH
Sbjct: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHHGRQYLKDCYIEGSVDFIFGNSTALLEHCH 180

Query: 181 VHCKSKGFITAQSRKSSQETTGYVFLRCVITGSGETSYVHLGRPWGPFARVVFAYTHMDV 240
           +HCKS+GFITAQSRKSSQETTGYVFLRCVITGSGETSYV+LGRPWGPFARVVFAYT+MD 
Sbjct: 181 IHCKSQGFITAQSRKSSQETTGYVFLRCVITGSGETSYVNLGRPWGPFARVVFAYTYMDA 240

Query: 241 CIKPAGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELLDEEADEFILHRFIDPD 300
           CIKP GWDNWGK ENERTACFYEYKCFGPGSCSMKRVCWAREL+DEEADEFILHRFIDPD
Sbjct: 241 CIKPTGWDNWGKTENERTACFYEYKCFGPGSCSMKRVCWARELVDEEADEFILHRFIDPD 300

Query: 301 VDRPWLCQRMALRIPFSA 318
           +++PWLC RMALR+PFSA
Sbjct: 301 IEKPWLCLRMALRLPFSA 318

[Close]

BLAST of CsGy5G028530 vs. NCBI nr
Match: XP_023551220.1 (pectinesterase 31 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023551221.1 pectinesterase 31 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 639 bits (1648), Expect = 3.66e-231
Identity = 294/318 (92.45%), Postives = 308/318 (96.86%), Query Frame = 0


Query: 1   MAAQPRVLTVAHDGCADFCTVQEAIDAVPFSNTCRTIIRVSPGIYKQPLYVPKTKNFITF 60
           MAA PRVLTVA DGCADF TVQEAIDAVPFSNTCRT+IRVSPGIY+QPLYVPKTKNFITF
Sbjct: 1   MAASPRVLTVAQDGCADFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60

Query: 61  AGLNPETTILTWDNTATKINHHQAARVIGTGTFGCGSTIVEGEDFLAENITFENSSPQGS 120
           AGLNPETT+LTWDNTATKI+HHQA+RVIGTGTFGCGSTIVEGEDF+AENITFENSSPQGS
Sbjct: 61  AGLNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120

Query: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYGRQYLKDCYIEGSVDFIFGNSTALLEHCH 180
           GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYG+QYLKDCY+EGSVDFIFGNSTALLE CH
Sbjct: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYGKQYLKDCYVEGSVDFIFGNSTALLERCH 180

Query: 181 VHCKSKGFITAQSRKSSQETTGYVFLRCVITGSGETSYVHLGRPWGPFARVVFAYTHMDV 240
           +HCKS GFITAQSRKSSQETTGYVFLRCVITGS  TSYVHLGRPWGPFARVVFAYT+MD 
Sbjct: 181 IHCKSHGFITAQSRKSSQETTGYVFLRCVITGSAGTSYVHLGRPWGPFARVVFAYTYMDA 240

Query: 241 CIKPAGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELLDEEADEFILHRFIDPD 300
           CIKPAGWDNWGK ENERTACFYEYKCFGPGSCSM RVCWAREL+DEEADEFILH+FIDPD
Sbjct: 241 CIKPAGWDNWGKTENERTACFYEYKCFGPGSCSMSRVCWARELVDEEADEFILHQFIDPD 300

Query: 301 VDRPWLCQRMALRIPFSA 318
           V+RPWLC+RMALRIPFSA
Sbjct: 301 VERPWLCRRMALRIPFSA 318

[Close]

BLAST of CsGy5G028530 vs. NCBI nr
Match: XP_022938497.1 (pectinesterase 31 [Cucurbita moschata])

HSP 1 Score: 638 bits (1645), Expect = 1.05e-230
Identity = 295/318 (92.77%), Postives = 307/318 (96.54%), Query Frame = 0


Query: 1   MAAQPRVLTVAHDGCADFCTVQEAIDAVPFSNTCRTIIRVSPGIYKQPLYVPKTKNFITF 60
           MAA PRVLTVA DGC DF TVQEAIDAVPFSNTCRT+IRVSPGIY+QPLYVPKTKNFITF
Sbjct: 1   MAAPPRVLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60

Query: 61  AGLNPETTILTWDNTATKINHHQAARVIGTGTFGCGSTIVEGEDFLAENITFENSSPQGS 120
           AGLNPETT+LTWDNTATKI+HHQA+RVIGTGTFGCGSTIVEGEDF+AENITFENSSPQGS
Sbjct: 61  AGLNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120

Query: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYGRQYLKDCYIEGSVDFIFGNSTALLEHCH 180
           GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYG+QYLKDCYIEGSVDFIFGNSTALLE CH
Sbjct: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYGKQYLKDCYIEGSVDFIFGNSTALLERCH 180

Query: 181 VHCKSKGFITAQSRKSSQETTGYVFLRCVITGSGETSYVHLGRPWGPFARVVFAYTHMDV 240
           VHCKS GFITAQSRKSSQETTGYVFLRCVITGS  TSYVHLGRPWGPFARVVFAYT+MD 
Sbjct: 181 VHCKSHGFITAQSRKSSQETTGYVFLRCVITGSAGTSYVHLGRPWGPFARVVFAYTYMDA 240

Query: 241 CIKPAGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELLDEEADEFILHRFIDPD 300
           CIKPAGWDNWGK ENERTACFYEYKCFGPGSCSM RVCWAREL+DEEADEFILH+FIDPD
Sbjct: 241 CIKPAGWDNWGKTENERTACFYEYKCFGPGSCSMSRVCWARELVDEEADEFILHQFIDPD 300

Query: 301 VDRPWLCQRMALRIPFSA 318
           V+RPWLC+RMALRIPFSA
Sbjct: 301 VERPWLCRRMALRIPFSA 318

[Close]

BLAST of CsGy5G028530 vs. ExPASy TrEMBL
Match: A0A0A0KVN5 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G637720 PE=4 SV=1)

HSP 1 Score: 680 bits (1754), Expect = 1.22e-247
Identity = 318/318 (100.00%), Postives = 318/318 (100.00%), Query Frame = 0


Query: 1   MAAQPRVLTVAHDGCADFCTVQEAIDAVPFSNTCRTIIRVSPGIYKQPLYVPKTKNFITF 60
           MAAQPRVLTVAHDGCADFCTVQEAIDAVPFSNTCRTIIRVSPGIYKQPLYVPKTKNFITF
Sbjct: 1   MAAQPRVLTVAHDGCADFCTVQEAIDAVPFSNTCRTIIRVSPGIYKQPLYVPKTKNFITF 60

Query: 61  AGLNPETTILTWDNTATKINHHQAARVIGTGTFGCGSTIVEGEDFLAENITFENSSPQGS 120
           AGLNPETTILTWDNTATKINHHQAARVIGTGTFGCGSTIVEGEDFLAENITFENSSPQGS
Sbjct: 61  AGLNPETTILTWDNTATKINHHQAARVIGTGTFGCGSTIVEGEDFLAENITFENSSPQGS 120

Query: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYGRQYLKDCYIEGSVDFIFGNSTALLEHCH 180
           GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYGRQYLKDCYIEGSVDFIFGNSTALLEHCH
Sbjct: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYGRQYLKDCYIEGSVDFIFGNSTALLEHCH 180

Query: 181 VHCKSKGFITAQSRKSSQETTGYVFLRCVITGSGETSYVHLGRPWGPFARVVFAYTHMDV 240
           VHCKSKGFITAQSRKSSQETTGYVFLRCVITGSGETSYVHLGRPWGPFARVVFAYTHMDV
Sbjct: 181 VHCKSKGFITAQSRKSSQETTGYVFLRCVITGSGETSYVHLGRPWGPFARVVFAYTHMDV 240

Query: 241 CIKPAGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELLDEEADEFILHRFIDPD 300
           CIKPAGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELLDEEADEFILHRFIDPD
Sbjct: 241 CIKPAGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELLDEEADEFILHRFIDPD 300

Query: 301 VDRPWLCQRMALRIPFSA 318
           VDRPWLCQRMALRIPFSA
Sbjct: 301 VDRPWLCQRMALRIPFSA 318

[Close]

BLAST of CsGy5G028530 vs. ExPASy TrEMBL
Match: A0A5A7TBP8 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G002680 PE=4 SV=1)

HSP 1 Score: 669 bits (1725), Expect = 3.23e-243
Identity = 312/318 (98.11%), Postives = 315/318 (99.06%), Query Frame = 0


Query: 1   MAAQPRVLTVAHDGCADFCTVQEAIDAVPFSNTCRTIIRVSPGIYKQPLYVPKTKNFITF 60
           MAAQPRVLTVAHDGCADF TVQEAIDAVPFSNTCRT+IRVSPGIYKQPLYVPKTKNFITF
Sbjct: 1   MAAQPRVLTVAHDGCADFRTVQEAIDAVPFSNTCRTVIRVSPGIYKQPLYVPKTKNFITF 60

Query: 61  AGLNPETTILTWDNTATKINHHQAARVIGTGTFGCGSTIVEGEDFLAENITFENSSPQGS 120
           AGLNPETTILTWDNTAT+I HHQAARVIGTGTFGCGSTIVEGEDF+AENITFENSSPQGS
Sbjct: 61  AGLNPETTILTWDNTATEIKHHQAARVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120

Query: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYGRQYLKDCYIEGSVDFIFGNSTALLEHCH 180
           GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYGRQYLKDCYIEGSVDFIFGNSTALLEHCH
Sbjct: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYGRQYLKDCYIEGSVDFIFGNSTALLEHCH 180

Query: 181 VHCKSKGFITAQSRKSSQETTGYVFLRCVITGSGETSYVHLGRPWGPFARVVFAYTHMDV 240
           VHCKSKGFITAQSRKSSQETTGYVFLRCVITGSGETSYVHLGRPWGPFARVVFAYTHMDV
Sbjct: 181 VHCKSKGFITAQSRKSSQETTGYVFLRCVITGSGETSYVHLGRPWGPFARVVFAYTHMDV 240

Query: 241 CIKPAGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELLDEEADEFILHRFIDPD 300
           CIKP GWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELLDEEADEFILHRFIDPD
Sbjct: 241 CIKPTGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELLDEEADEFILHRFIDPD 300

Query: 301 VDRPWLCQRMALRIPFSA 318
           VDRPWLCQRMALRIPFSA
Sbjct: 301 VDRPWLCQRMALRIPFSA 318

[Close]

BLAST of CsGy5G028530 vs. ExPASy TrEMBL
Match: A0A6J1FE78 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111444715 PE=4 SV=1)

HSP 1 Score: 638 bits (1645), Expect = 5.08e-231
Identity = 295/318 (92.77%), Postives = 307/318 (96.54%), Query Frame = 0


Query: 1   MAAQPRVLTVAHDGCADFCTVQEAIDAVPFSNTCRTIIRVSPGIYKQPLYVPKTKNFITF 60
           MAA PRVLTVA DGC DF TVQEAIDAVPFSNTCRT+IRVSPGIY+QPLYVPKTKNFITF
Sbjct: 1   MAAPPRVLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60

Query: 61  AGLNPETTILTWDNTATKINHHQAARVIGTGTFGCGSTIVEGEDFLAENITFENSSPQGS 120
           AGLNPETT+LTWDNTATKI+HHQA+RVIGTGTFGCGSTIVEGEDF+AENITFENSSPQGS
Sbjct: 61  AGLNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120

Query: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYGRQYLKDCYIEGSVDFIFGNSTALLEHCH 180
           GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYG+QYLKDCYIEGSVDFIFGNSTALLE CH
Sbjct: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYGKQYLKDCYIEGSVDFIFGNSTALLERCH 180

Query: 181 VHCKSKGFITAQSRKSSQETTGYVFLRCVITGSGETSYVHLGRPWGPFARVVFAYTHMDV 240
           VHCKS GFITAQSRKSSQETTGYVFLRCVITGS  TSYVHLGRPWGPFARVVFAYT+MD 
Sbjct: 181 VHCKSHGFITAQSRKSSQETTGYVFLRCVITGSAGTSYVHLGRPWGPFARVVFAYTYMDA 240

Query: 241 CIKPAGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELLDEEADEFILHRFIDPD 300
           CIKPAGWDNWGK ENERTACFYEYKCFGPGSCSM RVCWAREL+DEEADEFILH+FIDPD
Sbjct: 241 CIKPAGWDNWGKTENERTACFYEYKCFGPGSCSMSRVCWARELVDEEADEFILHQFIDPD 300

Query: 301 VDRPWLCQRMALRIPFSA 318
           V+RPWLC+RMALRIPFSA
Sbjct: 301 VERPWLCRRMALRIPFSA 318

[Close]

BLAST of CsGy5G028530 vs. ExPASy TrEMBL
Match: A0A6J1BZD5 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111006628 PE=4 SV=1)

HSP 1 Score: 635 bits (1638), Expect = 5.94e-230
Identity = 289/318 (90.88%), Postives = 307/318 (96.54%), Query Frame = 0


Query: 1   MAAQPRVLTVAHDGCADFCTVQEAIDAVPFSNTCRTIIRVSPGIYKQPLYVPKTKNFITF 60
           MAA PRV+TVAHDGC DF TVQEAIDAVPFSNTCRT+IRVSPGIY+QPLYVPKTKNFITF
Sbjct: 1   MAAPPRVITVAHDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60

Query: 61  AGLNPETTILTWDNTATKINHHQAARVIGTGTFGCGSTIVEGEDFLAENITFENSSPQGS 120
           AGL+PE+T+LTWDNTATKINHHQA+R+IGTGTFGCGSTIVEGEDF+AENITFENSSPQGS
Sbjct: 61  AGLSPESTVLTWDNTATKINHHQASRIIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120

Query: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYGRQYLKDCYIEGSVDFIFGNSTALLEHCH 180
           GQAVAIRVTADRCAFYNC+FLGWQDTLYLHYG+QYLKDCYIEGSVDFIFGNSTALLEHCH
Sbjct: 121 GQAVAIRVTADRCAFYNCKFLGWQDTLYLHYGKQYLKDCYIEGSVDFIFGNSTALLEHCH 180

Query: 181 VHCKSKGFITAQSRKSSQETTGYVFLRCVITGSGETSYVHLGRPWGPFARVVFAYTHMDV 240
           VHCKS GFITAQSRKSSQETTGYVFLRCVITGSG  SY+HLGRPWGPFARVVFAYT+MD 
Sbjct: 181 VHCKSPGFITAQSRKSSQETTGYVFLRCVITGSGGNSYIHLGRPWGPFARVVFAYTYMDA 240

Query: 241 CIKPAGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELLDEEADEFILHRFIDPD 300
           CIKPAGWDNWGK ENERTACFYEYKCFGPGSC MKRVCWAREL+DEE DEF+LH+FIDP+
Sbjct: 241 CIKPAGWDNWGKIENERTACFYEYKCFGPGSCQMKRVCWARELVDEETDEFLLHQFIDPN 300

Query: 301 VDRPWLCQRMALRIPFSA 318
           V+RPWLCQRMA RIPFSA
Sbjct: 301 VERPWLCQRMASRIPFSA 318

[Close]

BLAST of CsGy5G028530 vs. ExPASy TrEMBL
Match: A0A6J1JZ39 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111489575 PE=4 SV=1)

HSP 1 Score: 634 bits (1635), Expect = 1.70e-229
Identity = 292/318 (91.82%), Postives = 306/318 (96.23%), Query Frame = 0


Query: 1   MAAQPRVLTVAHDGCADFCTVQEAIDAVPFSNTCRTIIRVSPGIYKQPLYVPKTKNFITF 60
           MAA PRVLTVA DGC DF TVQEAIDAVPFSNTCRT+IRVSPGIY+QPLYVPKTKNFITF
Sbjct: 1   MAAPPRVLTVAQDGCGDFRTVQEAIDAVPFSNTCRTVIRVSPGIYRQPLYVPKTKNFITF 60

Query: 61  AGLNPETTILTWDNTATKINHHQAARVIGTGTFGCGSTIVEGEDFLAENITFENSSPQGS 120
           AGLNPETT+LTWDNTATKI+HHQA+RVIGTGTFGCGSTIVEGEDF+AENITFENSSPQGS
Sbjct: 61  AGLNPETTVLTWDNTATKISHHQASRVIGTGTFGCGSTIVEGEDFIAENITFENSSPQGS 120

Query: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYGRQYLKDCYIEGSVDFIFGNSTALLEHCH 180
           GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYG+QYLKDCYIEGSVDFIFGNSTALLE CH
Sbjct: 121 GQAVAIRVTADRCAFYNCRFLGWQDTLYLHYGKQYLKDCYIEGSVDFIFGNSTALLERCH 180

Query: 181 VHCKSKGFITAQSRKSSQETTGYVFLRCVITGSGETSYVHLGRPWGPFARVVFAYTHMDV 240
           +HCKS GFITAQSRKSSQETTGYVFLRCVITGS  +SYVHLGRPWGPFARVVFAYT+MD 
Sbjct: 181 IHCKSHGFITAQSRKSSQETTGYVFLRCVITGSAGSSYVHLGRPWGPFARVVFAYTYMDA 240

Query: 241 CIKPAGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELLDEEADEFILHRFIDPD 300
           CIKPAGWDNWGK ENERTACFYEYKCFGPGSC M RVCWAREL+DEEADEFILH+FIDPD
Sbjct: 241 CIKPAGWDNWGKTENERTACFYEYKCFGPGSCPMSRVCWARELVDEEADEFILHQFIDPD 300

Query: 301 VDRPWLCQRMALRIPFSA 318
           V+RPWLC+RMALRIPFSA
Sbjct: 301 VERPWLCRRMALRIPFSA 318

[Close]

BLAST of CsGy5G028530 vs. TAIR 10
Match: AT3G29090.1 (pectin methylesterase 31 )

HSP 1 Score: 560.8 bits (1444), Expect = 6.9e-160
Identity = 242/316 (76.58%), Postives = 286/316 (90.51%), Query Frame = 0


Query: 3   AQPRVLTVAHDGCADFCTVQEAIDAVPFSNTCRTIIRVSPGIYKQPLYVPKTKNFITFAG 62
           A  R++ V+ DG  D+C+VQ+AID+VP  NTCRT+IR+SPGIY+QP+YVPK KNFITFAG
Sbjct: 2   ATTRMVRVSQDGSGDYCSVQDAIDSVPLGNTCRTVIRLSPGIYRQPVYVPKRKNFITFAG 61

Query: 63  LNPETTILTWDNTATKINHHQAARVIGTGTFGCGSTIVEGEDFLAENITFENSSPQGSGQ 122
           ++PE T+LTW+NTA+KI HHQA+RVIGTGTFGCGS IVEGEDF+AENITFENS+P+GSGQ
Sbjct: 62  ISPEITVLTWNNTASKIEHHQASRVIGTGTFGCGSVIVEGEDFIAENITFENSAPEGSGQ 121

Query: 123 AVAIRVTADRCAFYNCRFLGWQDTLYLHYGRQYLKDCYIEGSVDFIFGNSTALLEHCHVH 182
           AVAIRVTADRCAFYNCRFLGWQDTLYLH+G+QYLKDCYIEGSVDFIFGNSTALLEHCH+H
Sbjct: 122 AVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLEHCHIH 181

Query: 183 CKSKGFITAQSRKSSQETTGYVFLRCVITGSGETSYVHLGRPWGPFARVVFAYTHMDVCI 242
           CKS+GFITAQSRKSSQE+TGYVFLRCVITG+G++ Y++LGRPWGPF RVV AYT+MD CI
Sbjct: 182 CKSQGFITAQSRKSSQESTGYVFLRCVITGNGQSGYMYLGRPWGPFGRVVLAYTYMDACI 241

Query: 243 KPAGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELLDEEADEFILHRFIDPDVD 302
           +  GW NWG AENER+ACFYEY+CFGPGSCS +RV W+REL+D+EA  F+ H F+DP+ D
Sbjct: 242 RNVGWHNWGNAENERSACFYEYRCFGPGSCSSERVPWSRELMDDEAGHFVHHSFVDPEQD 301

Query: 303 RPWLCQRMALRIPFSA 319
           RPWLC RM ++ P+SA
Sbjct: 302 RPWLCLRMGVKTPYSA 317

[Close]

BLAST of CsGy5G028530 vs. TAIR 10
Match: AT5G19730.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 272.3 bits (695), Expect = 4.9e-73
Identity = 140/289 (48.44%), Postives = 179/289 (61.94%), Query Frame = 0


Query: 17  DFCTVQEAIDAVPFSNTCRTIIRVSPGIYKQPLYVPKTKNFITFAGLNPETTILTWDNTA 76
           DF  +Q+AID++P  N  R +I+V  G+YK+ + +P  K FIT  G   E T + W +TA
Sbjct: 95  DFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVEWGDTA 154

Query: 77  TKINHHQAARVIGTGTFGCGSTIVEGEDFLAENITFENSSP-----QGSGQAVAIRVTAD 136
              +    ++    GT+   S  V    F+A+NITF N++P         QAVA+RV+AD
Sbjct: 155 QTPD----SKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSAD 214

Query: 137 RCAFYNCRFLGWQDTLYLHYGRQYLKDCYIEGSVDFIFGNSTALLEHCHVH--CKSKGFI 196
             AF+ CR LG QDTLY H GR Y KDCYIEGSVDFIFGN+ +L E CHVH      G +
Sbjct: 215 NAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGAV 274

Query: 197 TAQSRKSSQETTGYVFLRCVITGSGETSYVHLGRPWGPFARVVFAYTHMDVCIKPAGWDN 256
           TAQ R S  E TG+ F++C +TG+G    ++LGR WGPF+RVVFAYT+MD  I P GW N
Sbjct: 275 TAQGRSSVLEDTGFSFVKCKVTGTG---VLYLGRAWGPFSRVVFAYTYMDNIILPRGWYN 334

Query: 257 WGKAENERTACFYEYKCFGPGSCSMKRVCWARELLDEEADEFILHRFID 299
           WG    E T  + +YKC G G+    RV WAREL DEEA  F+   FID
Sbjct: 335 WGDPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLTFID 376

[Close]

BLAST of CsGy5G028530 vs. TAIR 10
Match: AT2G36710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 233.0 bits (593), Expect = 3.3e-61
Identity = 123/306 (40.20%), Postives = 177/306 (57.84%), Query Frame = 0


Query: 7   VLTVAHDGCADFCTVQEAIDAVPFSNTCRTIIRVSPGIYKQPLYVPKTKNFITFAGLNPE 66
           VLTV   GC +F  VQ AID VP  ++ +T+I V+ G Y++ + V + K  +   G   +
Sbjct: 90  VLTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENKTNLVIQGRGYQ 149

Query: 67  TTILTWDNTATKINHHQAARVIGTGTFGCGSTIVEGEDFLAENITFENSSP-----QGSG 126
            T + W++TA    +          T    S +V   +F A NI+F+N++P     +   
Sbjct: 150 NTSIEWNDTAKSAGN----------TADSFSFVVFAANFTAYNISFKNNAPEPDPGEADA 209

Query: 127 QAVAIRVTADRCAFYNCRFLGWQDTLYLHYGRQYLKDCYIEGSVDFIFGNSTALLEHCHV 186
           QAVA+R+  D+ AFY C F G QDTL    GR + K+C+I+GS+DFIFGN  +L + C +
Sbjct: 210 QAVALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRSLYQDCTI 269

Query: 187 HCKSK-------GFITAQSRKSSQETTGYVFLRCVITGSGETSYVHLGRPWGPFARVVFA 246
           +  +K       G ITAQ R+S  E +G+ F+ C I GSGE   + LGR WG +A VVF+
Sbjct: 270 NSIAKGNTSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGE---ILLGRAWGAYATVVFS 329

Query: 247 YTHMDVCIKPAGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELLDEEADEFILH 301
            T+M   I P GW+NWG +  E+T  F E+KC+GPG+   +RV + ++L D EA  FI  
Sbjct: 330 NTYMSGIITPEGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFGKQLTDSEASSFIDV 382

[Close]

BLAST of CsGy5G028530 vs. TAIR 10
Match: AT5G47500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 229.2 bits (583), Expect = 4.7e-60
Identity = 119/315 (37.78%), Postives = 180/315 (57.14%), Query Frame = 0


Query: 6   RVLTVAHDGCADFCTVQEAIDAVPFSNTCRTIIRVSPGIYKQPLYVPKTKNFITFAGLNP 65
           +V+TV+ +G A F +VQ+A+D++P +N     I+++PG Y++ + VP TK +ITF G   
Sbjct: 59  KVITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGR 118

Query: 66  ETTILTWDNTATKIN-HHQAARVIGTGTFGCGSTIVEGEDFLAENITFENSSP-----QG 125
           + T + W + A+ +  + Q  R     T+   S  V    F A NI+F N++P       
Sbjct: 119 DVTAIEWHDRASDLGANGQQLR-----TYQTASVTVYANYFTARNISFTNTAPAPLPGMQ 178

Query: 126 SGQAVAIRVTADRCAFYNCRFLGWQDTLYLHYGRQYLKDCYIEGSVDFIFGNSTALLEHC 185
             QAVA R++ D+  F  C F G QDTL    GR Y K+CYIEGS+DFIFGN  ++ + C
Sbjct: 179 GWQAVAFRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDC 238

Query: 186 HVHCKSK--GFITAQSRKSSQETTGYVFLRCVITGSGETSYVHLGRPWGPFARVVFAYTH 245
            +H  +   G I A  R   +E TG+ F+ C +TG+G    +++GR  G ++R+V+AYT+
Sbjct: 239 ELHSIASRFGSIAAHGRTCPEEKTGFAFVGCRVTGTGP---LYVGRAMGQYSRIVYAYTY 298

Query: 246 MDVCIKPAGWDNWG-KAENERTACFYEYKCFGPGSCSMKRVCWARELLDEEADEFILHRF 305
            D  +   GWD+W  K+   +TA F  Y C+GPG+ + + V WAR L  E A  FI   F
Sbjct: 299 FDALVAHGGWDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSF 358

Query: 306 IDPDVDRPWLCQRMA 312
           ++    R W+  R A
Sbjct: 359 VN---GRHWIAPRDA 362

[Close]

BLAST of CsGy5G028530 vs. TAIR 10
Match: AT2G21610.1 (pectinesterase 11 )

HSP 1 Score: 227.6 bits (579), Expect = 1.4e-59
Identity = 117/295 (39.66%), Postives = 167/295 (56.61%), Query Frame = 0


Query: 7   VLTVAHDGCADFCTVQEAIDAVP--FSNTCRTIIRVSPGIYKQPLYVPKTKNFITFAGLN 66
           ++ V   G  DF  +QEAI+++P   +N+    I V PGIY++ + +P  K +IT +G  
Sbjct: 50  LIRVDQSGKGDFSKIQEAIESIPPNLNNSQLYFIWVKPGIYREKVVIPAEKPYITLSGTQ 109

Query: 67  PETTILTWDNTATKINHHQAARVIGTGTFGCGSTIVEGEDFLAENITFENSSPQGSGQAV 126
              T L W +              G       +  +   DF+   +T +N     +G+AV
Sbjct: 110 ASNTFLIWSD--------------GEDILESPTLTIFASDFVCRFLTIQNKFGT-AGRAV 169

Query: 127 AIRVTADRCAFYNCRFLGWQDTLYLHYGRQYLKDCYIEGSVDFIFGNSTALLEHCHVHCK 186
           A+RV AD+ AFY C    +QDTL    G  Y K+CYIEG+ DFI G++++L E CH+H  
Sbjct: 170 ALRVAADKAAFYGCVITSYQDTLLDDNGNHYFKNCYIEGATDFICGSASSLYERCHLHSL 229

Query: 187 S--KGFITAQSRKSSQETTGYVFLRCVITGSGETSYVHLGRPWGPFARVVFAYTHMDVCI 246
           S   G ITAQ R S+ E +G+ FL C +TGSG T    LGRPWG ++RVVFAY+     +
Sbjct: 230 SPNNGSITAQMRTSATEKSGFTFLGCKLTGSGST---FLGRPWGAYSRVVFAYSFFSNVV 289

Query: 247 KPAGWDNWGKAENERTACFYEYKCFGPGSCSMKRVCWARELLDEEADEFILHRFI 298
            P GW+ WG +  E T  + EYKC+GPG+   +RV W+++L DEEA  F+   FI
Sbjct: 290 APQGWNQWGDSTKENTVYYGEYKCYGPGADREQRVEWSKQLSDEEATVFLSKDFI 326

The following BLAST results are available for this feature:

BLAST of CsGy5G028530 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LVQ0	9.6e-159	76.58	Pectinesterase 31 OS=Arabidopsis thaliana OX=3702 GN=PME31 PE=1 SV=1	[more]
Q8VYZ3	6.9e-72	48.44	Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1	[more]
Q9ZQA3	4.6e-60	40.20	Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1	[more]
Q8LPF3	6.7e-59	37.78	Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1	[more]
Q9SIJ9	1.9e-58	39.66	Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1	[more]

BLAST of CsGy5G028530 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004141450.1	2.52e-247	100.00	pectinesterase 31 [Cucumis sativus] >KGN52482.1 hypothetical protein Csa_007777 ...	[more]
KAA0039456.1	6.67e-243	98.11	pectinesterase 31 [Cucumis melo var. makuwa] >TYK00646.1 pectinesterase 31 [Cucu...	[more]
XP_038891224.1	2.69e-233	93.08	pectinesterase 31 [Benincasa hispida]	[more]
XP_023551220.1	3.66e-231	92.45	pectinesterase 31 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023551221.1 pectin...	[more]
XP_022938497.1	1.05e-230	92.77	pectinesterase 31 [Cucurbita moschata]	[more]

BLAST of CsGy5G028530 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KVN5	1.22e-247	100.00	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G637720 PE=4 SV=1	[more]
A0A5A7TBP8	3.23e-243	98.11	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G00268...	[more]
A0A6J1FE78	5.08e-231	92.77	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111444715 PE=4 SV=1	[more]
A0A6J1BZD5	5.94e-230	90.88	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111006628 PE=4 SV=1	[more]
A0A6J1JZ39	1.70e-229	91.82	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111489575 PE=4 SV=1	[more]

BLAST of CsGy5G028530 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G29090.1	6.9e-160	76.58	pectin methylesterase 31	[more]
AT5G19730.1	4.9e-73	48.44	Pectin lyase-like superfamily protein	[more]
AT2G36710.1	3.3e-61	40.20	Pectin lyase-like superfamily protein	[more]
AT5G47500.1	4.7e-60	37.78	Pectin lyase-like superfamily protein	[more]
AT2G21610.1	1.4e-59	39.66	pectinesterase 11	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 9..298 e-value: 2.6E-71 score: 239.8
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 3..310 e-value: 4.2E-106 score: 356.9
None	No IPR available	PANTHER	PTHR31321:SF71	PECTINESTERASE	coord: 6..317
None	No IPR available	PANTHER	PTHR31321	ACYL-COA THIOESTER HYDROLASE YBHC-RELATED	coord: 6..317
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 161..170
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 3..305

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsGy5G028530.1	CsGy5G028530.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0050829	defense response to Gram-negative bacterium
biological_process	GO:0045490	pectin catabolic process
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity