CsGy5G028420 (gene) Cucumber (Gy14) v2.1

Overview


Name	CsGy5G028420
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description	Calcium-binding EF-hand family protein
Location	Gy14Chr5: 32045689 .. 32047479 (+)
RNA-Seq Expression	CsGy5G028420
Synteny	CsGy5G028420

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGTGATTGATTAAAAAATAATTAAAAAACAACGGGAAGTAATTTCTCATTTTTAATTAAGATTATATTCTAAACCATATAATACATTTTATTTCCCTTTTCCTCTAAATATTTTCAATAAAAAAAAGTTTGGTAGAAAAGTCCAGAAATTTGACAGAATGACCTAACCGATCCACCCATCTTCCCTTCCATTCCCTTCCCTTCTCAAACGAAGCTTCCTATTCAACAACTCCCAATTTTGTTTACACATTTTTTTCTTCAATTAAACTAAATTTCTTTTGACTTTCTTCCCCAAAATTCCCCTGTTTCCTTCCTCTTTCAATCCCCACTAACGCGCAAAACAGAGCTTCTTCAAATACCCCTAATTCGACATTCATACTCCATCCAACAACAATCACAAAATTCATTCACAAATCACACAGCAATACAAAAATGATGAATCTCGGTCTCTACAACGTCTCCATGGACGACAAATCAACCCCACAAAACCCCTCTTTCCGATTCCTCTTAATCATCGACTTCCTCCGTTTTCAAAACCTTGCCTCTTGGCTCTGTTCCTCCACCTCTACAAAACTAAAACAGAGTACTCCTCCGGCGTCCTCAAAATCGGTGGAATCCAGAGCCGAGGAGAGGCTGGTGAGTACAGAGGAGATGAAATGGGTTATGGGGAATTTAGGGATTGATTGTAGCGAAGGGTCCTCTGTTCCTCTGTTTGGGTCAGCGGAAGAAGTGGCGGAGATGTTTGATCAAACGGCGGCTAGTGTAGCGGAGGTGAAACAAGCTTTTGGTGTGTTTGATGTTAATGGCGATGGGTTTATCGACGTTGAAGAATTGCAGAGAGTGATGTGTGTTTTGGGATTTAAAGAAGGAGAAGGGATTGAGAATTGCGAGAAGATGATTCGTAAGTTTGATTCTAACAAAGATGGGAGAATTGATTTTGAAGAATTTGTTAAATTGATGGAGGCCGTGGTTTGATTGTAATTGAAAAGATTGAATTTTTTTATTTATTGAATTGTTGTCTGTAGTGTGGTCGAAAATGAAGCCTTCTACCCTTTTTGTGGTGTTCTTTTTTTGGGTAAATGGAATTGAAACTTTGGCCCATTCTATTTCACTATGTAATTTTCATATACACACACATATATAATACAATGTTCACATATATTTATATCTTCAATCTTTTATTTTTATGTTTCTTCTCATTTTATTTTACCAATCAATAAGTATAAGGGGAAAAAAATCAATTCATCACACTAAAAATAGTTTATAGAGCTTTGTTGCTTGTTTTAAAACGTTGATTTGTCTCTGGGGGCTTTGTAGCTTATTATGGACAATTTTGTCCTTCCCTTCACCAAATCCAAGGAAAGTAAGATTTTGATTTTTTTTAAAAAAATTCTATTACTTTTGTATCATTCATGGGTAGGAAACTCCACGTTCATTAGTGTTGATAGCCTTCCAACTTATTCTTGAAGTTATGGTTTCTTAGATTTAAAAGGAAGGTGATCTCTCAATTTCTTCTCGTAGTCTACAAAAATGTATTTGATTTTTCAATGTTTGGAACTTATTCTACTTTATAGATTCTCTTAAAAGTCTTTTACCTCTACCTCAAGTTATCAATGGAGTATTTTAATCTTCACATAATTTCAAAATATTTGTGTCTTTTATATCTTTGAATCTTCGCGAAGCTTTGGGAATTTAGGACCCGTTTGATAATGTTCTCATCTCATGTTCTATGTTTTTGTTTTTCATTTTTTTTTTAAAAACAGACTATTTGATAACCAATCTTGTTTCTCAT

mRNA sequence

TGTGATTGATTAAAAAATAATTAAAAAACAACGGGAAGTAATTTCTCATTTTTAATTAAGATTATATTCTAAACCATATAATACATTTTATTTCCCTTTTCCTCTAAATATTTTCAATAAAAAAAAGTTTGGTAGAAAAGTCCAGAAATTTGACAGAATGACCTAACCGATCCACCCATCTTCCCTTCCATTCCCTTCCCTTCTCAAACGAAGCTTCCTATTCAACAACTCCCAATTTTGTTTACACATTTTTTTCTTCAATTAAACTAAATTTCTTTTGACTTTCTTCCCCAAAATTCCCCTGTTTCCTTCCTCTTTCAATCCCCACTAACGCGCAAAACAGAGCTTCTTCAAATACCCCTAATTCGACATTCATACTCCATCCAACAACAATCACAAAATTCATTCACAAATCACACAGCAATACAAAAATGATGAATCTCGGTCTCTACAACGTCTCCATGGACGACAAATCAACCCCACAAAACCCCTCTTTCCGATTCCTCTTAATCATCGACTTCCTCCGTTTTCAAAACCTTGCCTCTTGGCTCTGTTCCTCCACCTCTACAAAACTAAAACAGAGTACTCCTCCGGCGTCCTCAAAATCGGTGGAATCCAGAGCCGAGGAGAGGCTGGTGAGTACAGAGGAGATGAAATGGGTTATGGGGAATTTAGGGATTGATTGTAGCGAAGGGTCCTCTGTTCCTCTGTTTGGGTCAGCGGAAGAAGTGGCGGAGATGTTTGATCAAACGGCGGCTAGTGTAGCGGAGGTGAAACAAGCTTTTGGTGTGTTTGATGTTAATGGCGATGGGTTTATCGACGTTGAAGAATTGCAGAGAGTGATGTGTGTTTTGGGATTTAAAGAAGGAGAAGGGATTGAGAATTGCGAGAAGATGATTCGTAAGTTTGATTCTAACAAAGATGGGAGAATTGATTTTGAAGAATTTGTTAAATTGATGGAGGCCGTGGTTTGATTGTAATTGAAAAGATTGAATTTTTTTATTTATTGAATTGTTGTCTGTAGTGTGGTCGAAAATGAAGCCTTCTACCCTTTTTGTGGTGTTCTTTTTTTGGGTAAATGGAATTGAAACTTTGGCCCATTCTATTTCACTATGTAATTTTCATATACACACACATATATAATACAATGTTCACATATATTTATATCTTCAATCTTTTATTTTTATGTTTCTTCTCATTTTATTTTACCAATCAATAAGTATAAGGGGAAAAAAATCAATTCATCACACTAAAAATAGTTTATAGAGCTTTGTTGCTTGTTTTAAAACGTTGATTTGTCTCTGGGGGCTTTGTAGCTTATTATGGACAATTTTGTCCTTCCCTTCACCAAATCCAAGGAAAGTAAGATTTTGATTTTTTTTAAAAAAATTCTATTACTTTTGTATCATTCATGGGTAGGAAACTCCACGTTCATTAGTGTTGATAGCCTTCCAACTTATTCTTGAAGTTATGGTTTCTTAGATTTAAAAGGAAGGTGATCTCTCAATTTCTTCTCGTAGTCTACAAAAATGTATTTGATTTTTCAATGTTTGGAACTTATTCTACTTTATAGATTCTCTTAAAAGTCTTTTACCTCTACCTCAAGTTATCAATGGAGTATTTTAATCTTCACATAATTTCAAAATATTTGTGTCTTTTATATCTTTGAATCTTCGCGAAGCTTTGGGAATTTAGGACCCGTTTGATAATGTTCTCATCTCATGTTCTATGTTTTTGTTTTTCATTTTTTTTTTAAAAACAGACTATTTGATAACCAATCTTGTTTCTCAT

Coding sequence (CDS)

ATGATGAATCTCGGTCTCTACAACGTCTCCATGGACGACAAATCAACCCCACAAAACCCCTCTTTCCGATTCCTCTTAATCATCGACTTCCTCCGTTTTCAAAACCTTGCCTCTTGGCTCTGTTCCTCCACCTCTACAAAACTAAAACAGAGTACTCCTCCGGCGTCCTCAAAATCGGTGGAATCCAGAGCCGAGGAGAGGCTGGTGAGTACAGAGGAGATGAAATGGGTTATGGGGAATTTAGGGATTGATTGTAGCGAAGGGTCCTCTGTTCCTCTGTTTGGGTCAGCGGAAGAAGTGGCGGAGATGTTTGATCAAACGGCGGCTAGTGTAGCGGAGGTGAAACAAGCTTTTGGTGTGTTTGATGTTAATGGCGATGGGTTTATCGACGTTGAAGAATTGCAGAGAGTGATGTGTGTTTTGGGATTTAAAGAAGGAGAAGGGATTGAGAATTGCGAGAAGATGATTCGTAAGTTTGATTCTAACAAAGATGGGAGAATTGATTTTGAAGAATTTGTTAAATTGATGGAGGCCGTGGTTTGA

Protein sequence

MMNLGLYNVSMDDKSTPQNPSFRFLLIIDFLRFQNLASWLCSSTSTKLKQSTPPASSKSVESRAEERLVSTEEMKWVMGNLGIDCSEGSSVPLFGSAEEVAEMFDQTAASVAEVKQAFGVFDVNGDGFIDVEELQRVMCVLGFKEGEGIENCEKMIRKFDSNKDGRIDFEEFVKLMEAVV*

Homology

[Close]

BLAST of CsGy5G028420 vs. ExPASy Swiss-Prot
Match: Q9MBG5 (Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 PE=2 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 5.2e-24
Identity = 58/109 (53.21%), Postives = 77/109 (70.64%), Query Frame = 0


Query: 69  VSTEEMKWVMGNLGIDCSEGSSVPLFGSAEEVAEMFDQTAASVAEVKQAFGVFDVNGDGF 128
           +S EE + VM +LG+  ++      + SA+EV+ +F++  AS+ EVKQAF VFD N DGF
Sbjct: 83  ISREEAEMVMRSLGLFYNDDQLQEQY-SAKEVSSLFEEKEASLEEVKQAFDVFDENKDGF 142

Query: 129 IDVEELQRVMCVLGFKEGEGIENCEKMIRKFDSNKDGRIDFEEFVKLME 178
           ID  ELQRV+ +LGFK+G  ++NC  MIR  D NKDG+IDF EFVK ME
Sbjct: 143 IDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKFME 190

[Close]

BLAST of CsGy5G028420 vs. ExPASy Swiss-Prot
Match: Q93Z27 (Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 PE=1 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 6.3e-22
Identity = 54/107 (50.47%), Postives = 73/107 (68.22%), Query Frame = 0


Query: 72  EEMKWVMGNLGIDC-SEGSSVPLFGSAEEVAEMFDQTAASVAEVKQAFGVFDVNGDGFID 131
           E++  VM +LG+    E   +    S++EV+ +F++   S+ EVKQAF VFD N DGFID
Sbjct: 94  EDVGMVMKSLGLSTDQENEGLQKQYSSKEVSNLFEEKEPSLEEVKQAFDVFDENRDGFID 153

Query: 132 VEELQRVMCVLGFKEGEGIENCEKMIRKFDSNKDGRIDFEEFVKLME 178
             +LQRV+ +LG K+G  +ENC +MIR FD +KDGRIDF  FVK ME
Sbjct: 154 PIDLQRVLTILGLKQGSNLENCRRMIRSFDGSKDGRIDFYGFVKFME 200

[Close]

BLAST of CsGy5G028420 vs. ExPASy Swiss-Prot
Match: Q9LNE7 (Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 1.4e-13
Identity = 37/95 (38.95%), Postives = 62/95 (65.26%), Query Frame = 0


Query: 83  IDCSEGSSVPLFGSAEEVAEMFDQTAASVAEVKQAFGVFDVNGDGFIDVEELQRVMCVLG 142
           ID +    V +    E    + D+      ++K+AF VFD NGDGFI V+EL+ V+  LG
Sbjct: 49  IDVNGDGCVDIDEFGELYKTIMDEEDEEEEDMKEAFNVFDQNGDGFITVDELKAVLSSLG 108

Query: 143 FKEGEGIENCEKMIRKFDSNKDGRIDFEEFVKLME 178
            K+G+ +++C+KMI+K D + DGR++++EF ++M+
Sbjct: 109 LKQGKTLDDCKKMIKKVDVDGDGRVNYKEFRQMMK 143

[Close]

BLAST of CsGy5G028420 vs. ExPASy Swiss-Prot
Match: Q9SVG9 (Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 2.4e-13
Identity = 51/122 (41.80%), Postives = 65/122 (53.28%), Query Frame = 0


Query: 68  LVSTEEMKWVMGNLGIDCSEGSSV------PLFGSAEEVAEMFD---QTAASVAEVKQAF 127
           L ST E     GN G++  + SS+        FG A    E  D     A + +++ +AF
Sbjct: 66  LKSTVESYIQPGNTGLNFDDFSSLHKTLDDSFFGGACGGGENEDDPSSAAENESDLAEAF 125

Query: 128 GVFDVNGDGFIDVEELQRVMCVLGFKEGEGIENCEKMIRKFDSNKDGRIDFEEFVKLMEA 181
            VFD NGDGFI   ELQ V+  LG  EG  +E  EKMI   D N+DGR+DF EF  +M  
Sbjct: 126 KVFDENGDGFISARELQTVLKKLGLPEGGEMERVEKMIVSVDRNQDGRVDFFEFKNMMRT 185

[Close]

BLAST of CsGy5G028420 vs. ExPASy Swiss-Prot
Match: Q9ZR02 (Calmodulin-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=CML6 PE=2 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 2.4e-13
Identity = 36/110 (32.73%), Postives = 66/110 (60.00%), Query Frame = 0


Query: 68  LVSTEEMKWVMGNLGIDCSEGSSVPLFGSAEEVAEMFDQTAASVAEVKQAFGVFDVNGDG 127
           ++  +E+  ++  + ++      +  FG   +   + D+      ++K+AF VFD NGDG
Sbjct: 36  IIPEDELTQIIQKIDVNGDGCVDIEEFGELYKTIMVEDEDEVGEEDMKEAFNVFDRNGDG 95

Query: 128 FIDVEELQRVMCVLGFKEGEGIENCEKMIRKFDSNKDGRIDFEEFVKLME 178
           FI V+EL+ V+  LG K+G+ +E C KMI + D + DGR+++ EF ++M+
Sbjct: 96  FITVDELKAVLSSLGLKQGKTLEECRKMIMQVDVDGDGRVNYMEFRQMMK 145

[Close]

BLAST of CsGy5G028420 vs. NCBI nr
Match: XP_031741770.1 (probable calcium-binding protein CML47 [Cucumis sativus])

HSP 1 Score: 355 bits (912), Expect = 1.35e-121
Identity = 180/180 (100.00%), Postives = 180/180 (100.00%), Query Frame = 0


Query: 1   MMNLGLYNVSMDDKSTPQNPSFRFLLIIDFLRFQNLASWLCSSTSTKLKQSTPPASSKSV 60
           MMNLGLYNVSMDDKSTPQNPSFRFLLIIDFLRFQNLASWLCSSTSTKLKQSTPPASSKSV
Sbjct: 131 MMNLGLYNVSMDDKSTPQNPSFRFLLIIDFLRFQNLASWLCSSTSTKLKQSTPPASSKSV 190

Query: 61  ESRAEERLVSTEEMKWVMGNLGIDCSEGSSVPLFGSAEEVAEMFDQTAASVAEVKQAFGV 120
           ESRAEERLVSTEEMKWVMGNLGIDCSEGSSVPLFGSAEEVAEMFDQTAASVAEVKQAFGV
Sbjct: 191 ESRAEERLVSTEEMKWVMGNLGIDCSEGSSVPLFGSAEEVAEMFDQTAASVAEVKQAFGV 250

Query: 121 FDVNGDGFIDVEELQRVMCVLGFKEGEGIENCEKMIRKFDSNKDGRIDFEEFVKLMEAVV 180
           FDVNGDGFIDVEELQRVMCVLGFKEGEGIENCEKMIRKFDSNKDGRIDFEEFVKLMEAVV
Sbjct: 251 FDVNGDGFIDVEELQRVMCVLGFKEGEGIENCEKMIRKFDSNKDGRIDFEEFVKLMEAVV 310

[Close]

BLAST of CsGy5G028420 vs. NCBI nr
Match: XP_008459700.2 (PREDICTED: probable calcium-binding protein CML30 [Cucumis melo])

HSP 1 Score: 322 bits (824), Expect = 3.87e-110
Identity = 166/181 (91.71%), Postives = 168/181 (92.82%), Query Frame = 0


Query: 2   MNLGLYNVSMDDKSTPQNPSFRFLLIIDFLRFQNLASWLCSSTSTKLKQSTPPASSKSVE 61
           MN GLYNVSMDDKSTPQNPSFRFL+IIDFLRFQNLASWLCS  STKLKQST PASSKSVE
Sbjct: 1   MNFGLYNVSMDDKSTPQNPSFRFLIIIDFLRFQNLASWLCSFASTKLKQSTLPASSKSVE 60

Query: 62  SRA--EERLVSTEEMKWVMGNLGIDCSEGSSVPLFGSAEEVAEMFDQTAASVAEVKQAFG 121
           S A   ERLVSTEEMKWVMGNLG+DCSEGSSVPLFGSAEEVAEMFDQTAAS AEVKQAF 
Sbjct: 61  SSAGERERLVSTEEMKWVMGNLGMDCSEGSSVPLFGSAEEVAEMFDQTAASAAEVKQAFD 120

Query: 122 VFDVNGDGFIDVEELQRVMCVLGFKEGEGIENCEKMIRKFDSNKDGRIDFEEFVKLMEAV 180
           VFDVN DGFIDVEELQRVMCVLGFKEGEGIENCEKMI KFD NKDGRIDFEEFVKLMEAV
Sbjct: 121 VFDVNADGFIDVEELQRVMCVLGFKEGEGIENCEKMISKFDYNKDGRIDFEEFVKLMEAV 180

[Close]

BLAST of CsGy5G028420 vs. NCBI nr
Match: KAA0039468.1 (putative calcium-binding protein CML30 [Cucumis melo var. makuwa] >TYK00658.1 putative calcium-binding protein CML30 [Cucumis melo var. makuwa])

HSP 1 Score: 308 bits (789), Expect = 6.12e-105
Identity = 159/172 (92.44%), Postives = 161/172 (93.60%), Query Frame = 0


Query: 11  MDDKSTPQNPSFRFLLIIDFLRFQNLASWLCSSTSTKLKQSTPPASSKSVESRA--EERL 70
           MDDKSTPQNPSFRFL+IIDFLRFQNLASWLCS  STKLKQST PASSKSVES A   ERL
Sbjct: 1   MDDKSTPQNPSFRFLIIIDFLRFQNLASWLCSFASTKLKQSTLPASSKSVESSAGERERL 60

Query: 71  VSTEEMKWVMGNLGIDCSEGSSVPLFGSAEEVAEMFDQTAASVAEVKQAFGVFDVNGDGF 130
           VSTEEMKWVMGNLG+DCSEGSSVPLFGSAEEVAEMFDQTAAS AEVKQAF VFDVNGDGF
Sbjct: 61  VSTEEMKWVMGNLGMDCSEGSSVPLFGSAEEVAEMFDQTAASAAEVKQAFDVFDVNGDGF 120

Query: 131 IDVEELQRVMCVLGFKEGEGIENCEKMIRKFDSNKDGRIDFEEFVKLMEAVV 180
           IDVEELQRVMCVLGFKEGEGIENCEKMI KFD NKDGRIDFEEFVKLMEAVV
Sbjct: 121 IDVEELQRVMCVLGFKEGEGIENCEKMISKFDYNKDGRIDFEEFVKLMEAVV 172

[Close]

BLAST of CsGy5G028420 vs. NCBI nr
Match: KAE8648926.1 (hypothetical protein Csa_007983 [Cucumis sativus])

HSP 1 Score: 289 bits (740), Expect = 1.27e-97
Identity = 146/146 (100.00%), Postives = 146/146 (100.00%), Query Frame = 0


Query: 11  MDDKSTPQNPSFRFLLIIDFLRFQNLASWLCSSTSTKLKQSTPPASSKSVESRAEERLVS 70
           MDDKSTPQNPSFRFLLIIDFLRFQNLASWLCSSTSTKLKQSTPPASSKSVESRAEERLVS
Sbjct: 1   MDDKSTPQNPSFRFLLIIDFLRFQNLASWLCSSTSTKLKQSTPPASSKSVESRAEERLVS 60

Query: 71  TEEMKWVMGNLGIDCSEGSSVPLFGSAEEVAEMFDQTAASVAEVKQAFGVFDVNGDGFID 130
           TEEMKWVMGNLGIDCSEGSSVPLFGSAEEVAEMFDQTAASVAEVKQAFGVFDVNGDGFID
Sbjct: 61  TEEMKWVMGNLGIDCSEGSSVPLFGSAEEVAEMFDQTAASVAEVKQAFGVFDVNGDGFID 120

Query: 131 VEELQRVMCVLGFKEGEGIENCEKMI 156
           VEELQRVMCVLGFKEGEGIENCEKMI
Sbjct: 121 VEELQRVMCVLGFKEGEGIENCEKMI 146

[Close]

BLAST of CsGy5G028420 vs. NCBI nr
Match: XP_038889669.1 (probable calcium-binding protein CML46 [Benincasa hispida])

HSP 1 Score: 270 bits (690), Expect = 9.14e-90
Identity = 136/179 (75.98%), Postives = 154/179 (86.03%), Query Frame = 0


Query: 2   MNLGLYNVSMDDKSTPQNPSFRFLLIIDFLRFQNLASWLCSSTSTKLKQSTPPASSKSVE 61
           M+ G+YNVSMDDK+T QNPSFRFL++IDFLRFQ LASWL +  +TK K S+ PA+SK+ E
Sbjct: 1   MDFGVYNVSMDDKTTTQNPSFRFLIVIDFLRFQKLASWLYAFATTKQKPSSFPATSKTEE 60

Query: 62  SRAEERLVSTEEMKWVMGNLGIDCSEGSSVPLFGSAEEVAEMFDQTAASVAEVKQAFGVF 121
              EERL++TEEMKWVMGNLGIDC E  SVPLFGS EEVAEMFD+ AAS  EVK+AF VF
Sbjct: 61  HGGEERLMTTEEMKWVMGNLGIDCGE-ESVPLFGSTEEVAEMFDERAASTVEVKKAFDVF 120

Query: 122 DVNGDGFIDVEELQRVMCVLGFKEGEGIENCEKMIRKFDSNKDGRIDFEEFVKLMEAVV 180
           DVNGDGFID EELQRVMCVLGFKEGE I+NCE+MIRKFD N DGRIDFEEF+KLME+VV
Sbjct: 121 DVNGDGFIDSEELQRVMCVLGFKEGERIQNCERMIRKFDHNGDGRIDFEEFLKLMESVV 178

[Close]

BLAST of CsGy5G028420 vs. ExPASy TrEMBL
Match: A0A0A0KS94 (Calcium ion binding protein OS=Cucumis sativus OX=3659 GN=Csa_5G636620 PE=4 SV=1)

HSP 1 Score: 355 bits (912), Expect = 6.92e-124
Identity = 180/180 (100.00%), Postives = 180/180 (100.00%), Query Frame = 0


Query: 1   MMNLGLYNVSMDDKSTPQNPSFRFLLIIDFLRFQNLASWLCSSTSTKLKQSTPPASSKSV 60
           MMNLGLYNVSMDDKSTPQNPSFRFLLIIDFLRFQNLASWLCSSTSTKLKQSTPPASSKSV
Sbjct: 1   MMNLGLYNVSMDDKSTPQNPSFRFLLIIDFLRFQNLASWLCSSTSTKLKQSTPPASSKSV 60

Query: 61  ESRAEERLVSTEEMKWVMGNLGIDCSEGSSVPLFGSAEEVAEMFDQTAASVAEVKQAFGV 120
           ESRAEERLVSTEEMKWVMGNLGIDCSEGSSVPLFGSAEEVAEMFDQTAASVAEVKQAFGV
Sbjct: 61  ESRAEERLVSTEEMKWVMGNLGIDCSEGSSVPLFGSAEEVAEMFDQTAASVAEVKQAFGV 120

Query: 121 FDVNGDGFIDVEELQRVMCVLGFKEGEGIENCEKMIRKFDSNKDGRIDFEEFVKLMEAVV 180
           FDVNGDGFIDVEELQRVMCVLGFKEGEGIENCEKMIRKFDSNKDGRIDFEEFVKLMEAVV
Sbjct: 121 FDVNGDGFIDVEELQRVMCVLGFKEGEGIENCEKMIRKFDSNKDGRIDFEEFVKLMEAVV 180

[Close]

BLAST of CsGy5G028420 vs. ExPASy TrEMBL
Match: A0A1S3CAB3 (probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103498740 PE=4 SV=1)

HSP 1 Score: 322 bits (824), Expect = 1.87e-110
Identity = 166/181 (91.71%), Postives = 168/181 (92.82%), Query Frame = 0


Query: 2   MNLGLYNVSMDDKSTPQNPSFRFLLIIDFLRFQNLASWLCSSTSTKLKQSTPPASSKSVE 61
           MN GLYNVSMDDKSTPQNPSFRFL+IIDFLRFQNLASWLCS  STKLKQST PASSKSVE
Sbjct: 1   MNFGLYNVSMDDKSTPQNPSFRFLIIIDFLRFQNLASWLCSFASTKLKQSTLPASSKSVE 60

Query: 62  SRA--EERLVSTEEMKWVMGNLGIDCSEGSSVPLFGSAEEVAEMFDQTAASVAEVKQAFG 121
           S A   ERLVSTEEMKWVMGNLG+DCSEGSSVPLFGSAEEVAEMFDQTAAS AEVKQAF 
Sbjct: 61  SSAGERERLVSTEEMKWVMGNLGMDCSEGSSVPLFGSAEEVAEMFDQTAASAAEVKQAFD 120

Query: 122 VFDVNGDGFIDVEELQRVMCVLGFKEGEGIENCEKMIRKFDSNKDGRIDFEEFVKLMEAV 180
           VFDVN DGFIDVEELQRVMCVLGFKEGEGIENCEKMI KFD NKDGRIDFEEFVKLMEAV
Sbjct: 121 VFDVNADGFIDVEELQRVMCVLGFKEGEGIENCEKMISKFDYNKDGRIDFEEFVKLMEAV 180

[Close]

BLAST of CsGy5G028420 vs. ExPASy TrEMBL
Match: A0A5A7T9C9 (Putative calcium-binding protein CML30 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G002800 PE=4 SV=1)

HSP 1 Score: 308 bits (789), Expect = 2.96e-105
Identity = 159/172 (92.44%), Postives = 161/172 (93.60%), Query Frame = 0


Query: 11  MDDKSTPQNPSFRFLLIIDFLRFQNLASWLCSSTSTKLKQSTPPASSKSVESRA--EERL 70
           MDDKSTPQNPSFRFL+IIDFLRFQNLASWLCS  STKLKQST PASSKSVES A   ERL
Sbjct: 1   MDDKSTPQNPSFRFLIIIDFLRFQNLASWLCSFASTKLKQSTLPASSKSVESSAGERERL 60

Query: 71  VSTEEMKWVMGNLGIDCSEGSSVPLFGSAEEVAEMFDQTAASVAEVKQAFGVFDVNGDGF 130
           VSTEEMKWVMGNLG+DCSEGSSVPLFGSAEEVAEMFDQTAAS AEVKQAF VFDVNGDGF
Sbjct: 61  VSTEEMKWVMGNLGMDCSEGSSVPLFGSAEEVAEMFDQTAASAAEVKQAFDVFDVNGDGF 120

Query: 131 IDVEELQRVMCVLGFKEGEGIENCEKMIRKFDSNKDGRIDFEEFVKLMEAVV 180
           IDVEELQRVMCVLGFKEGEGIENCEKMI KFD NKDGRIDFEEFVKLMEAVV
Sbjct: 121 IDVEELQRVMCVLGFKEGEGIENCEKMISKFDYNKDGRIDFEEFVKLMEAVV 172

[Close]

BLAST of CsGy5G028420 vs. ExPASy TrEMBL
Match: A0A6J1JZ70 (probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC111489598 PE=4 SV=1)

HSP 1 Score: 204 bits (518), Expect = 5.94e-64
Identity = 108/174 (62.07%), Postives = 128/174 (73.56%), Query Frame = 0


Query: 11  MDDKSTPQNPSFRFLLIIDFLRFQNLASWLCSSTSTKLKQSTPPAS--SKSVESRAEERL 70
           MD  + P NPSFRFL++ID  RFQ L SWL +  S K KQ + PAS  +KS + +     
Sbjct: 1   MDGNTIPANPSFRFLIMIDCFRFQKLGSWLYAFASGKRKQWSLPASETAKSEQRKEGGER 60

Query: 71  VSTEEMKWVMGNLGIDCSEGS--SVPLFGSAEEVAEMFDQTAASVAEVKQAFGVFDVNGD 130
           VS EEMKWVM  LG+   E +  S+P F S EE+ ++FDQTAAS AEVK+AF VFDVN D
Sbjct: 61  VSREEMKWVMEELGLSWGEETEESLPGFASMEEMCKIFDQTAASAAEVKEAFDVFDVNSD 120

Query: 131 GFIDVEELQRVMCVLGFKEGEGIENCEKMIRKFDSNKDGRIDFEEFVKLMEAVV 180
           GFIDVEELQRV+CVLGFKEGE I+NC +MI KFD N DGRIDF+EFVKLME+ V
Sbjct: 121 GFIDVEELQRVLCVLGFKEGEAIQNCRRMINKFDENNDGRIDFQEFVKLMESAV 174

[Close]

BLAST of CsGy5G028420 vs. ExPASy TrEMBL
Match: A0A6J1FKT4 (probable calcium-binding protein CML45 OS=Cucurbita moschata OX=3662 GN=LOC111445127 PE=4 SV=1)

HSP 1 Score: 198 bits (503), Expect = 1.13e-61
Identity = 104/174 (59.77%), Postives = 125/174 (71.84%), Query Frame = 0


Query: 11  MDDKSTPQNPSFRFLLIIDFLRFQNLASWLCSSTSTKLKQSTPPASSKSVESRAEE--RL 70
           MD  + P  PSFRFL++ID  RFQ L SWL +  + K KQ + PAS  +   + +E    
Sbjct: 1   MDGNTIPAIPSFRFLIMIDCFRFQKLGSWLYAFATGKRKQWSLPASETAKSEQRDEGGER 60

Query: 71  VSTEEMKWVMGNLGIDCSEGS--SVPLFGSAEEVAEMFDQTAASVAEVKQAFGVFDVNGD 130
           VS EEMKWVMG LG+   E +  S+P F S EE+  +FDQTAAS AEVK+AF VFDVN D
Sbjct: 61  VSREEMKWVMGELGLSSEEETEESLPGFASMEEMCRIFDQTAASAAEVKEAFDVFDVNSD 120

Query: 131 GFIDVEELQRVMCVLGFKEGEGIENCEKMIRKFDSNKDGRIDFEEFVKLMEAVV 180
           GFID +ELQRV+CVLGFKEGE I+NC +MI KFD N DGRIDF+EFVKLME+ V
Sbjct: 121 GFIDGDELQRVLCVLGFKEGEAIQNCRRMINKFDENNDGRIDFQEFVKLMESAV 174

[Close]

BLAST of CsGy5G028420 vs. TAIR 10
Match: AT3G29000.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 112.5 bits (280), Expect = 3.7e-25
Identity = 58/109 (53.21%), Postives = 77/109 (70.64%), Query Frame = 0


Query: 69  VSTEEMKWVMGNLGIDCSEGSSVPLFGSAEEVAEMFDQTAASVAEVKQAFGVFDVNGDGF 128
           +S EE + VM +LG+  ++      + SA+EV+ +F++  AS+ EVKQAF VFD N DGF
Sbjct: 83  ISREEAEMVMRSLGLFYNDDQLQEQY-SAKEVSSLFEEKEASLEEVKQAFDVFDENKDGF 142

Query: 129 IDVEELQRVMCVLGFKEGEGIENCEKMIRKFDSNKDGRIDFEEFVKLME 178
           ID  ELQRV+ +LGFK+G  ++NC  MIR  D NKDG+IDF EFVK ME
Sbjct: 143 IDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKFME 190

[Close]

BLAST of CsGy5G028420 vs. TAIR 10
Match: AT5G39670.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 105.5 bits (262), Expect = 4.5e-23
Identity = 54/107 (50.47%), Postives = 73/107 (68.22%), Query Frame = 0


Query: 72  EEMKWVMGNLGIDC-SEGSSVPLFGSAEEVAEMFDQTAASVAEVKQAFGVFDVNGDGFID 131
           E++  VM +LG+    E   +    S++EV+ +F++   S+ EVKQAF VFD N DGFID
Sbjct: 94  EDVGMVMKSLGLSTDQENEGLQKQYSSKEVSNLFEEKEPSLEEVKQAFDVFDENRDGFID 153

Query: 132 VEELQRVMCVLGFKEGEGIENCEKMIRKFDSNKDGRIDFEEFVKLME 178
             +LQRV+ +LG K+G  +ENC +MIR FD +KDGRIDF  FVK ME
Sbjct: 154 PIDLQRVLTILGLKQGSNLENCRRMIRSFDGSKDGRIDFYGFVKFME 200

[Close]

BLAST of CsGy5G028420 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 77.8 bits (190), Expect = 1.0e-14
Identity = 37/95 (38.95%), Postives = 62/95 (65.26%), Query Frame = 0


Query: 83  IDCSEGSSVPLFGSAEEVAEMFDQTAASVAEVKQAFGVFDVNGDGFIDVEELQRVMCVLG 142
           ID +    V +    E    + D+      ++K+AF VFD NGDGFI V+EL+ V+  LG
Sbjct: 49  IDVNGDGCVDIDEFGELYKTIMDEEDEEEEDMKEAFNVFDQNGDGFITVDELKAVLSSLG 108

Query: 143 FKEGEGIENCEKMIRKFDSNKDGRIDFEEFVKLME 178
            K+G+ +++C+KMI+K D + DGR++++EF ++M+
Sbjct: 109 LKQGKTLDDCKKMIKKVDVDGDGRVNYKEFRQMMK 143

[Close]

BLAST of CsGy5G028420 vs. TAIR 10
Match: AT4G03290.1 (EF hand calcium-binding protein family )

HSP 1 Score: 77.0 bits (188), Expect = 1.7e-14
Identity = 36/110 (32.73%), Postives = 66/110 (60.00%), Query Frame = 0


Query: 68  LVSTEEMKWVMGNLGIDCSEGSSVPLFGSAEEVAEMFDQTAASVAEVKQAFGVFDVNGDG 127
           ++  +E+  ++  + ++      +  FG   +   + D+      ++K+AF VFD NGDG
Sbjct: 36  IIPEDELTQIIQKIDVNGDGCVDIEEFGELYKTIMVEDEDEVGEEDMKEAFNVFDRNGDG 95

Query: 128 FIDVEELQRVMCVLGFKEGEGIENCEKMIRKFDSNKDGRIDFEEFVKLME 178
           FI V+EL+ V+  LG K+G+ +E C KMI + D + DGR+++ EF ++M+
Sbjct: 96  FITVDELKAVLSSLGLKQGKTLEECRKMIMQVDVDGDGRVNYMEFRQMMK 145

[Close]

BLAST of CsGy5G028420 vs. TAIR 10
Match: AT4G20780.1 (calmodulin like 42 )

HSP 1 Score: 77.0 bits (188), Expect = 1.7e-14
Identity = 51/122 (41.80%), Postives = 65/122 (53.28%), Query Frame = 0


Query: 68  LVSTEEMKWVMGNLGIDCSEGSSV------PLFGSAEEVAEMFD---QTAASVAEVKQAF 127
           L ST E     GN G++  + SS+        FG A    E  D     A + +++ +AF
Sbjct: 66  LKSTVESYIQPGNTGLNFDDFSSLHKTLDDSFFGGACGGGENEDDPSSAAENESDLAEAF 125

Query: 128 GVFDVNGDGFIDVEELQRVMCVLGFKEGEGIENCEKMIRKFDSNKDGRIDFEEFVKLMEA 181
            VFD NGDGFI   ELQ V+  LG  EG  +E  EKMI   D N+DGR+DF EF  +M  
Sbjct: 126 KVFDENGDGFISARELQTVLKKLGLPEGGEMERVEKMIVSVDRNQDGRVDFFEFKNMMRT 185

The following BLAST results are available for this feature:

BLAST of CsGy5G028420 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9MBG5	5.2e-24	53.21	Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 ...	[more]
Q93Z27	6.3e-22	50.47	Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 ...	[more]
Q9LNE7	1.4e-13	38.95	Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1	[more]
Q9SVG9	2.4e-13	41.80	Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1	[more]
Q9ZR02	2.4e-13	32.73	Calmodulin-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=CML6 PE=2 SV=1	[more]

BLAST of CsGy5G028420 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031741770.1	1.35e-121	100.00	probable calcium-binding protein CML47 [Cucumis sativus]	[more]
XP_008459700.2	3.87e-110	91.71	PREDICTED: probable calcium-binding protein CML30 [Cucumis melo]	[more]
KAA0039468.1	6.12e-105	92.44	putative calcium-binding protein CML30 [Cucumis melo var. makuwa] >TYK00658.1 pu...	[more]
KAE8648926.1	1.27e-97	100.00	hypothetical protein Csa_007983 [Cucumis sativus]	[more]
XP_038889669.1	9.14e-90	75.98	probable calcium-binding protein CML46 [Benincasa hispida]	[more]

BLAST of CsGy5G028420 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KS94	6.92e-124	100.00	Calcium ion binding protein OS=Cucumis sativus OX=3659 GN=Csa_5G636620 PE=4 SV=1	[more]
A0A1S3CAB3	1.87e-110	91.71	probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103498740 P...	[more]
A0A5A7T9C9	2.96e-105	92.44	Putative calcium-binding protein CML30 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A6J1JZ70	5.94e-64	62.07	probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC1114895...	[more]
A0A6J1FKT4	1.13e-61	59.77	probable calcium-binding protein CML45 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]

BLAST of CsGy5G028420 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G29000.1	3.7e-25	53.21	Calcium-binding EF-hand family protein	[more]
AT5G39670.1	4.5e-23	50.47	Calcium-binding EF-hand family protein	[more]
AT1G05990.1	1.0e-14	38.95	EF hand calcium-binding protein family	[more]
AT4G03290.1	1.7e-14	32.73	EF hand calcium-binding protein family	[more]
AT4G20780.1	1.7e-14	41.80	calmodulin like 42	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 113..141 e-value: 7.1E-5 score: 32.2 coord: 151..179 e-value: 2.6E-6 score: 37.0
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 114..177 e-value: 6.4E-14 score: 52.1
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 109..144 score: 15.043417
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 147..180 score: 15.82449
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 113..176 e-value: 3.4266E-19 score: 74.8917
None	No IPR available	GENE3D	1.10.238.10		coord: 94..178 e-value: 1.1E-21 score: 79.0
None	No IPR available	PANTHER	PTHR10891:SF745	CALCIUM-BINDING PROTEIN CML46-RELATED	coord: 10..177
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 10..177
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 160..172
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 122..134
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 68..177

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsGy5G028420.1	CsGy5G028420.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding