CsGy5G025485 (gene) Cucumber (Gy14) v2.1

Overview


Name	CsGy5G025485
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like
Location	Gy14Chr5: 29700193 .. 29702305 (-)
RNA-Seq Expression	CsGy5G025485
Synteny	CsGy5G025485

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCGTTTCATTCCTACCCATACCTTTTGAAGCTCTTTCCCATCTGGGTTTCGGCGGCAATGGACGTTTTGTCGGCGTTTCTCTTCCCTCCGCCTCCTTCCACGTTCGTCAATGTGATGACGGTAGTTAGCACGACGGCGTTGGCTATGGCTGGCCTTTCCGAGATCAACGGTAAGCATTTGCAGTATTCCAAGTTCTGGAATGCAAATGGTGCTTCGAAACTTTCAGCCGAAGAATCTAAGGTTTCGTCCAAATACGGAATGCTTTTGGCTTATACTCCGGCGTTTCTAGCTGGCGCCGCGTCGTTTTGGCTCTTTCCGAGCGGTGATGAGAGGGTTTTTCTGCTTAAATCTGCTCTCACTCTCCATTTCTTCAAGAGGATATTTGAGGTACTGTCTCTCTCTCTCTCTCTCTCTCTTTAAGCCTTTGTTTGAGTTCTGAAATTTCAAATCTGATTCTGGAATTAGGGATTACAGAATGCAGTTCTTCTCTGATGTTCTTGAGTACAGCGAAATTCATATCCTCGTAACTTCATTTGTTCGATTCGATTGGGTGTTTATTTAGAAGTTATTAATCTTATTCTTAAAAAAATTATCGGGTTCTTGAACACTGTATGAAAATTCGTGGCGAAGAGAATGTTGTAGAGAGAATTGAATCCTTTTTTCCTTCTCTGCTTCTTATCATTCCGTTGGAGATATCAAGTTTGGAACTCTGTTCTTCTTTGTTTACAGGCTTGAAAAACTTTTGACCCAATTTTTTTTTTAATTCATTGTTTGCATATATTATGAATTGGGGTTTCGAACTTTGACTTAGGCTCACTTTGGCGGTTGTCTTATTATCAATTTTGATGAGTACTTTTGTTTTTTTCTTTTGACCAAGGATGGCTTAATCAAAATCTCGAGTTCCAAGTTGCTAGTTGAGCTATGCCCATTTTAACAATAACCCATTTGCTTAGAGACAATCTGACTTTATTAATTTTACTCTTTGACTAATCTAATTATCATGAATATATTCGACTATTGTACTTTGTATAGAATGTTCAGACTGATTATTATATTAAGTCATGCTTAAGAAAGAAACAAACAACACGATTCTGTTCAGCAGTTCTCAAAAAATTATGTTTCACTAATCAACAATGTGATTGCAAGTCTTATACCTTTCATCCCTCGAACTATTCTTCTTTGTTCTTGATTATTTTTTTCTTTTTCACTCCATTTCAGGTGAAAGGTTGAACTATGAAACTTTGAAATAGTGTAACAGATATCTTGCAAATTATATGAGCTATTTAGAAAACAATGCAAAATGTTTTAAAAAATTAAGCTGATGGCCATGTTGAGTTTAATTATTTCAATACTTTACACTCTTCTTCACTTGTAAAGTTAGAAATTTTGGAATGCTTTGAATCTGTTACATGACTCTTCAAATGACTAACCTCGGGATCTCACTCTATTTGTTTAACGATTTTAGGCTAAAAGTTTAGAGAGCTGTCATATATAAACTCTCTAGTAATAATTTGTCCTTTCTCTATGGTCTACACCTACTCGCATTGTTTCTTTGTTGATTTTGTAACAAAGAAGTTCAACTACTATTATCTTCAACTACATCTTTGAAGTAGTGGTGTGTTCTTGAAAATCATGACAGGTACTGTTTATTCACAAATACAGCAGTAAAATGGTATTGAAGACAGCAATTCAAATCTCCTTAAGCTACTTCATGTCCACAGCAATCATGATATACACTCAGCACCTTTCACAAGGGCTTCCAGAGCCACCCATTGATCTGAAAAACATTGGAGTTGGTTCGTTTCTTATAGGCATTATTGGGAATTTTTACCATCATTATCTCTTGTCACAAACAAGAAAACAAGGAGACACAGCCTACAAAATTCCCAAAGGAGGCCTCTTCAGCTTTGTCGTTTGCCCTCATTATCTGTTTGAAATAATTGGGTATTTTGGATTTGCATTCATTTCTCAGGAGCTTTATCCTTTGTGCTTCAGCATTGGCACTGCTGTTTATTTGGCTGGGAGGAGCTATGCCACTAGGAAATGGTACATCTCCAAATTTGAAGCTTTCCCCAACAATGTCAAAGCTCTTCTCCCTTTTGTCTTTTAG

mRNA sequence

ATGCCGTTTCATTCCTACCCATACCTTTTGAAGCTCTTTCCCATCTGGGTTTCGGCGGCAATGGACGTTTTGTCGGCGTTTCTCTTCCCTCCGCCTCCTTCCACGTTCGTCAATGTGATGACGGTAGTTAGCACGACGGCGTTGGCTATGGCTGGCCTTTCCGAGATCAACGGTAAGCATTTGCAGTATTCCAAGTTCTGGAATGCAAATGGTGCTTCGAAACTTTCAGCCGAAGAATCTAAGGTTTCGTCCAAATACGGAATGCTTTTGGCTTATACTCCGGCGTTTCTAGCTGGCGCCGCGTCGTTTTGGCTCTTTCCGAGCGGTGATGAGAGGGTTTTTCTGCTTAAATCTGCTCTCACTCTCCATTTCTTCAAGAGGATATTTGAGGTACTGTTTATTCACAAATACAGCAGTAAAATGGTATTGAAGACAGCAATTCAAATCTCCTTAAGCTACTTCATGTCCACAGCAATCATGATATACACTCAGCACCTTTCACAAGGGCTTCCAGAGCCACCCATTGATCTGAAAAACATTGGAGTTGGTTCGTTTCTTATAGGCATTATTGGGAATTTTTACCATCATTATCTCTTGTCACAAACAAGAAAACAAGGAGACACAGCCTACAAAATTCCCAAAGGAGGCCTCTTCAGCTTTGTCGTTTGCCCTCATTATCTGTTTGAAATAATTGGGTATTTTGGATTTGCATTCATTTCTCAGGAGCTTTATCCTTTGTGCTTCAGCATTGGCACTGCTGTTTATTTGGCTGGGAGGAGCTATGCCACTAGGAAATGGTACATCTCCAAATTTGAAGCTTTCCCCAACAATGTCAAAGCTCTTCTCCCTTTTGTCTTTTAG

Coding sequence (CDS)

ATGCCGTTTCATTCCTACCCATACCTTTTGAAGCTCTTTCCCATCTGGGTTTCGGCGGCAATGGACGTTTTGTCGGCGTTTCTCTTCCCTCCGCCTCCTTCCACGTTCGTCAATGTGATGACGGTAGTTAGCACGACGGCGTTGGCTATGGCTGGCCTTTCCGAGATCAACGGTAAGCATTTGCAGTATTCCAAGTTCTGGAATGCAAATGGTGCTTCGAAACTTTCAGCCGAAGAATCTAAGGTTTCGTCCAAATACGGAATGCTTTTGGCTTATACTCCGGCGTTTCTAGCTGGCGCCGCGTCGTTTTGGCTCTTTCCGAGCGGTGATGAGAGGGTTTTTCTGCTTAAATCTGCTCTCACTCTCCATTTCTTCAAGAGGATATTTGAGGTACTGTTTATTCACAAATACAGCAGTAAAATGGTATTGAAGACAGCAATTCAAATCTCCTTAAGCTACTTCATGTCCACAGCAATCATGATATACACTCAGCACCTTTCACAAGGGCTTCCAGAGCCACCCATTGATCTGAAAAACATTGGAGTTGGTTCGTTTCTTATAGGCATTATTGGGAATTTTTACCATCATTATCTCTTGTCACAAACAAGAAAACAAGGAGACACAGCCTACAAAATTCCCAAAGGAGGCCTCTTCAGCTTTGTCGTTTGCCCTCATTATCTGTTTGAAATAATTGGGTATTTTGGATTTGCATTCATTTCTCAGGAGCTTTATCCTTTGTGCTTCAGCATTGGCACTGCTGTTTATTTGGCTGGGAGGAGCTATGCCACTAGGAAATGGTACATCTCCAAATTTGAAGCTTTCCCCAACAATGTCAAAGCTCTTCTCCCTTTTGTCTTTTAG

Protein sequence

MPFHSYPYLLKLFPIWVSAAMDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNANGASKLSAEESKVSSKYGMLLAYTPAFLAGAASFWLFPSGDERVFLLKSALTLHFFKRIFEVLFIHKYSSKMVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSFLIGIIGNFYHHYLLSQTRKQGDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFSIGTAVYLAGRSYATRKWYISKFEAFPNNVKALLPFVF*

Homology

[Close]

BLAST of CsGy5G025485 vs. ExPASy Swiss-Prot
Match: Q28891 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis OX=9541 GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 3.9e-18
Identity = 43/107 (40.19%), Postives = 65/107 (60.75%), Query Frame = 0


Query: 180 IGVGSFLIGIIGNFYHHYLLSQTRKQGDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFI 239
           IG G +L G++ N +  ++L   RK GDT YKIP+GGLF +V   +Y  EI+ + G+A  
Sbjct: 157 IGFGLWLAGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIMEWCGYALA 216

Query: 240 SQELYPLCFSIGTAVYLAGRSYATRKWYISKFEAFPNNVKALLPFVF 287
           S  +    F+  T  +L+GR+    +WY+ KFE +P   K L+PF+F
Sbjct: 217 SWSVQGAAFAFFTFCFLSGRAKEHHRWYLQKFEEYPKFRKILIPFLF 263

[Close]

BLAST of CsGy5G025485 vs. ExPASy Swiss-Prot
Match: P18405 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens OX=9606 GN=SRD5A1 PE=1 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 1.1e-17
Identity = 42/107 (39.25%), Postives = 65/107 (60.75%), Query Frame = 0


Query: 180 IGVGSFLIGIIGNFYHHYLLSQTRKQGDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFI 239
           IG G +L G++ N +  ++L   RK GDT YKIP+GGLF +V   +Y  EI+ + G+A  
Sbjct: 153 IGFGLWLTGMLINIHSDHILRNLRKPGDTGYKIPRGGLFEYVTAANYFGEIMEWCGYALA 212

Query: 240 SQELYPLCFSIGTAVYLAGRSYATRKWYISKFEAFPNNVKALLPFVF 287
           S  +    F+  T  +L+GR+    +WY+ KFE +P   K ++PF+F
Sbjct: 213 SWSVQGAAFAFFTFCFLSGRAKEHHEWYLRKFEEYPKFRKIIIPFLF 259

[Close]

BLAST of CsGy5G025485 vs. ExPASy Swiss-Prot
Match: Q55C17 (Very-long-chain enoyl-CoA reductase OS=Dictyostelium discoideum OX=44689 GN=gpsn2 PE=3 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 5.7e-17
Identity = 55/185 (29.73%), Postives = 96/185 (51.89%), Query Frame = 0


Query: 105 LFPSGDERVFLLKSAL---TLHFFKRIFEVLFIHKYSSKMVLKTAIQISLSYFMSTAIMI 164
           ++ +   + F  K AL   +LH+ KRI+E +F+H++S   +    +  + SY+     M+
Sbjct: 115 IYGTDSPKSFAQKVALVCYSLHYIKRIYETIFVHRFSHGTMPIFNLFKNCSYYWGCTAMV 174

Query: 165 YTQHLSQGLPEPPIDLKNIGVGSFLIGIIGNFYHHYLLSQTRKQGDTAYKIPKGGLFSFV 224
                     E PI+   +G+G ++IG + N+  H  L   R  G T  KIP+G LF FV
Sbjct: 175 SYFVNHPLYTEAPIERVYLGLGLWIIGEVFNYICHIQLRNLRPAGSTERKIPRGLLFEFV 234

Query: 225 VCPHYLFEIIGYFGFAFISQELYPLCFSIGTAVYLAGRSYATRKWYISKF-EAFPNNVKA 284
            CP+Y  EI+ + GF+ ++Q L    F++  A  +   +    + Y  +F + +P + K 
Sbjct: 235 SCPNYTVEILSWIGFSILTQTLTSWIFALMGAAQMWIWAVGKHRRYRKEFGDKYPKSRKI 294

Query: 285 LLPFV 286
           L+PF+
Sbjct: 295 LIPFL 299

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BLAST of CsGy5G025485 vs. ExPASy Swiss-Prot
Match: Q2QDF6 (Steroid 5-alpha-reductase DET2 OS=Gossypium hirsutum OX=3635 GN=DET2 PE=1 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 9.7e-17
Identity = 63/200 (31.50%), Postives = 104/200 (52.00%), Query Frame = 0


Query: 102 SFWLFPSG----DERVFLLKSALTLHFFKRIFEVLFIHKYSSKMVLKTAIQISLSYFMST 161
           +F+LFPSG    + + FLL S    H+F R   VL+  + +          +S++ FM+ 
Sbjct: 62  TFFLFPSGQHFYNPKSFLLISPFLFHYFNR--TVLYPLRLARNTTQTRGFPVSVA-FMAF 121

Query: 162 AIMIYTQHL-SQGLPEPPIDLKN---------IGVGSFLIGIIGNFYHHYLLSQTRKQGD 221
              +   +L ++ +     D +N          G+  F++G+  N     +L   +KQGD
Sbjct: 122 GFNLLNGYLQARWVSHYKDDYENEELFWWRFLAGLLIFVVGMWVNVRADKVLVGLKKQGD 181

Query: 222 TAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFSIGTAVYLAGRSYATRKWY 281
             YKIP+GGLF  V CP+Y  EI+ +FG+A ++       F + T   L  R+ ATR WY
Sbjct: 182 GGYKIPRGGLFELVSCPNYFGEIMEWFGWAVMTWSWVGFGFFLYTCANLMPRARATRLWY 241

Query: 282 ISKF-EAFPNNVKALLPFVF 287
           + KF + +P + KA++PF++
Sbjct: 242 LEKFKDDYPKDRKAVIPFIY 258

[Close]

BLAST of CsGy5G025485 vs. ExPASy Swiss-Prot
Match: Q28892 (3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Macaca fascicularis OX=9541 GN=SRD5A2 PE=1 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 2.8e-16
Identity = 52/176 (29.55%), Postives = 96/176 (54.55%), Query Frame = 0


Query: 115 LLKSALTLHFFKRIFEVLFIHK---YSSKMVLKTAIQISLSYFMSTAIMIYTQHLSQGLP 174
           +L     +H+F R F    +++   Y + ++ +     + + F+ +  +IY      G  
Sbjct: 82  VLLGLFCVHYFHRTFVYSLLNRGRPYPAVLIFRGIAFCAGNGFLQSYYLIYCAEYPDGW- 141

Query: 175 EPPIDLK-NIGVGSFLIGIIGNFYHHYLLSQTRKQGDTAYKIPKGGLFSFVVCPHYLFEI 234
               D++  +GV  F++G+  N +  Y+L Q RK G+  Y+IPKGGLF++V   ++L EI
Sbjct: 142 --YTDIRFCLGVFLFILGMGVNIHSDYILRQLRKPGEITYRIPKGGLFTYVSGANFLGEI 201

Query: 235 IGYFGFAFISQELYPLCFSIGTAVYLAGRSYATRKWYISKFEAFPNNVKALLPFVF 287
           I + G+A  +  L  L F+  +  +L  R++   ++Y+  FE +P + KAL+PF+F
Sbjct: 202 IEWIGYALATWSLPALAFAFFSVCFLGLRAFHHHRFYLKMFEDYPKSRKALIPFIF 254

[Close]

BLAST of CsGy5G025485 vs. NCBI nr
Match: XP_004135052.2 (3-oxo-5-alpha-steroid 4-dehydrogenase 2 [Cucumis sativus] >KAE8648808.1 hypothetical protein Csa_008329 [Cucumis sativus])

HSP 1 Score: 567 bits (1462), Expect = 6.91e-204
Identity = 286/286 (100.00%), Postives = 286/286 (100.00%), Query Frame = 0


Query: 1   MPFHSYPYLLKLFPIWVSAAMDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKH 60
           MPFHSYPYLLKLFPIWVSAAMDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKH
Sbjct: 1   MPFHSYPYLLKLFPIWVSAAMDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKH 60

Query: 61  LQYSKFWNANGASKLSAEESKVSSKYGMLLAYTPAFLAGAASFWLFPSGDERVFLLKSAL 120
           LQYSKFWNANGASKLSAEESKVSSKYGMLLAYTPAFLAGAASFWLFPSGDERVFLLKSAL
Sbjct: 61  LQYSKFWNANGASKLSAEESKVSSKYGMLLAYTPAFLAGAASFWLFPSGDERVFLLKSAL 120

Query: 121 TLHFFKRIFEVLFIHKYSSKMVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNI 180
           TLHFFKRIFEVLFIHKYSSKMVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNI
Sbjct: 121 TLHFFKRIFEVLFIHKYSSKMVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNI 180

Query: 181 GVGSFLIGIIGNFYHHYLLSQTRKQGDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFIS 240
           GVGSFLIGIIGNFYHHYLLSQTRKQGDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFIS
Sbjct: 181 GVGSFLIGIIGNFYHHYLLSQTRKQGDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFIS 240

Query: 241 QELYPLCFSIGTAVYLAGRSYATRKWYISKFEAFPNNVKALLPFVF 286
           QELYPLCFSIGTAVYLAGRSYATRKWYISKFEAFPNNVKALLPFVF
Sbjct: 241 QELYPLCFSIGTAVYLAGRSYATRKWYISKFEAFPNNVKALLPFVF 286

[Close]

BLAST of CsGy5G025485 vs. NCBI nr
Match: XP_008446722.1 (PREDICTED: 3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Cucumis melo])

HSP 1 Score: 510 bits (1313), Expect = 2.46e-181
Identity = 253/274 (92.34%), Postives = 263/274 (95.99%), Query Frame = 0


Query: 13  FPIWVSAAMDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNANGA 72
           FPIWVS AMD+LSAFLFPPPPSTFVNVMTVVS+T+LAMAGLSEI GKHLQYSKFWNAN  
Sbjct: 3   FPIWVSVAMDILSAFLFPPPPSTFVNVMTVVSSTSLAMAGLSEIKGKHLQYSKFWNANSG 62

Query: 73  SKLSAEESKVSSKYGMLLAYTPAFLAGAASFWLFPSGDERVFLLKSALTLHFFKRIFEVL 132
           SKLSA+ESK+SSKYGMLL YTPAFLAGAASFWLFPSGDER FLLKSALTLHFFKRIFEVL
Sbjct: 63  SKLSAQESKLSSKYGMLLVYTPAFLAGAASFWLFPSGDERFFLLKSALTLHFFKRIFEVL 122

Query: 133 FIHKYSSKMVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSFLIGIIGN 192
           FIHKYSSKMVLKTAIQIS SYF+STAIMIYTQHLSQGLPEPPIDLKNIGVG FL GIIGN
Sbjct: 123 FIHKYSSKMVLKTAIQISFSYFLSTAIMIYTQHLSQGLPEPPIDLKNIGVGLFLTGIIGN 182

Query: 193 FYHHYLLSQTRKQGDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFSIGT 252
           FYHHYLLSQTRKQ +TAYKIPKGGLFSFV+CPHYLFEIIGYFGFAFISQELYPLCFSIGT
Sbjct: 183 FYHHYLLSQTRKQEETAYKIPKGGLFSFVICPHYLFEIIGYFGFAFISQELYPLCFSIGT 242

Query: 253 AVYLAGRSYATRKWYISKFEAFPNNVKALLPFVF 286
           A+YLAGRSYATRKWYISKFE FPN+VKALLPFVF
Sbjct: 243 AIYLAGRSYATRKWYISKFENFPNHVKALLPFVF 276

[Close]

BLAST of CsGy5G025485 vs. NCBI nr
Match: XP_038891781.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Benincasa hispida])

HSP 1 Score: 492 bits (1267), Expect = 5.81e-174
Identity = 245/281 (87.19%), Postives = 265/281 (94.31%), Query Frame = 0


Query: 6   YPYLLKLFPIWVSAAMDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSK 65
           Y  LLKLFPI +SAAMDVLSAFLFPPPPS FVN+MTVVS+TALAMAG+SEINGKHLQYSK
Sbjct: 19  YTRLLKLFPIGISAAMDVLSAFLFPPPPSKFVNLMTVVSSTALAMAGISEINGKHLQYSK 78

Query: 66  FWNANGASKLSAEESKVSSKYGMLLAYTPAFLAGAASFWLFPSGDERVFLLKSALTLHFF 125
           FWNAN A KLS+E S +SSKYGMLLAYTPAFLAGAASFWLFPS DER+ LLKSALTLHFF
Sbjct: 79  FWNANTAPKLSSEVSMLSSKYGMLLAYTPAFLAGAASFWLFPSADERILLLKSALTLHFF 138

Query: 126 KRIFEVLFIHKYSSKMVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSF 185
           KRIFEVLFIHKYSSKMVL TAIQIS SYF+ST IMIYTQHLSQGLPEPPI+LKNIG+G F
Sbjct: 139 KRIFEVLFIHKYSSKMVLVTAIQISFSYFLSTVIMIYTQHLSQGLPEPPIELKNIGIGLF 198

Query: 186 LIGIIGNFYHHYLLSQTRKQGDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYP 245
           LIGIIGNFYHHYLLS+TRK+G+T+Y+IPKGGLFSFV+CPHYLFEII +FGFAFISQELYP
Sbjct: 199 LIGIIGNFYHHYLLSKTRKEGETSYEIPKGGLFSFVICPHYLFEIIEFFGFAFISQELYP 258

Query: 246 LCFSIGTAVYLAGRSYATRKWYISKFEAFPNNVKALLPFVF 286
           LCFSIGT++YLAGRSYATRKWYISKFE FPN+VKALLPFVF
Sbjct: 259 LCFSIGTSIYLAGRSYATRKWYISKFENFPNHVKALLPFVF 299

[Close]

BLAST of CsGy5G025485 vs. NCBI nr
Match: XP_022957108.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita moschata])

HSP 1 Score: 452 bits (1163), Expect = 1.22e-158
Identity = 220/266 (82.71%), Postives = 243/266 (91.35%), Query Frame = 0


Query: 21  MDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNANGASKLSAEES 80
           M +LSAFLFPPPPSTFVNVMTVVS+TA+ MAG+SEINGKHLQYSKFWNAN + K  +EE 
Sbjct: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60

Query: 81  KVSSKYGMLLAYTPAFLAGAASFWLFPSGDERVFLLKSALTLHFFKRIFEVLFIHKYSSK 140
           K+SSKYGMLLAYTPAFLAGAASFW FPS +ER+ +LK+ALTLHFFKRIFEVLFIHKYSSK
Sbjct: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120

Query: 141 MVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSFLIGIIGNFYHHYLLS 200
           MV  TAI+IS SY +ST IMIYTQHLSQGLPEPPIDLKNIG+  FLIGIIGNFYHH+LLS
Sbjct: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180

Query: 201 QTRKQGDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFSIGTAVYLAGRS 260
           +TRK G+T YKIPKGGLFS V+CPHYLFEII +FGFAFISQELYPLCFS GTA+YLAGRS
Sbjct: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240

Query: 261 YATRKWYISKFEAFPNNVKALLPFVF 286
           YATRKWY+SKFE FP++VKALLPFVF
Sbjct: 241 YATRKWYVSKFENFPSHVKALLPFVF 266

[Close]

BLAST of CsGy5G025485 vs. NCBI nr
Match: XP_022984951.1 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita maxima])

HSP 1 Score: 451 bits (1161), Expect = 2.46e-158
Identity = 219/266 (82.33%), Postives = 243/266 (91.35%), Query Frame = 0


Query: 21  MDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNANGASKLSAEES 80
           M +LSAFLFPPPPSTFVNVMTVVS+TA+ MAG+SEINGKHLQYSKFWNAN + K  +EE 
Sbjct: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60

Query: 81  KVSSKYGMLLAYTPAFLAGAASFWLFPSGDERVFLLKSALTLHFFKRIFEVLFIHKYSSK 140
           K+SSKYGMLLAYTPAFLAGAASFW FPS +ER+ +LK+ALTLHFFKRIFEVLFIHKYSSK
Sbjct: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120

Query: 141 MVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSFLIGIIGNFYHHYLLS 200
           MV  TAI+IS SY +ST IMIYTQHLSQG+PEPPIDLKNIG+  FLIGIIGNFYHH+LLS
Sbjct: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGIPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180

Query: 201 QTRKQGDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFSIGTAVYLAGRS 260
           +TRK G+T YKIPKGGLFS V+CPHYLFEII +FGFAFISQELYPLCFS GTA+YLAGRS
Sbjct: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240

Query: 261 YATRKWYISKFEAFPNNVKALLPFVF 286
           YATRKWY+SKFE FP++VKALLPFVF
Sbjct: 241 YATRKWYVSKFENFPSHVKALLPFVF 266

[Close]

BLAST of CsGy5G025485 vs. ExPASy TrEMBL
Match: A0A0A0KTC7 (S5A_REDUCTASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G612880 PE=3 SV=1)

HSP 1 Score: 525 bits (1353), Expect = 7.11e-188
Identity = 268/286 (93.71%), Postives = 268/286 (93.71%), Query Frame = 0


Query: 1   MPFHSYPYLLKLFPIWVSAAMDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKH 60
           MPFHSYPYLLKLFPIWVSAAMDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKH
Sbjct: 1   MPFHSYPYLLKLFPIWVSAAMDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKH 60

Query: 61  LQYSKFWNANGASKLSAEESKVSSKYGMLLAYTPAFLAGAASFWLFPSGDERVFLLKSAL 120
           LQYSKFWNANGASKLSAEESKVSSKYGMLLAYTPAFLAGAASFWLFPSGDERV       
Sbjct: 61  LQYSKFWNANGASKLSAEESKVSSKYGMLLAYTPAFLAGAASFWLFPSGDERV------- 120

Query: 121 TLHFFKRIFEVLFIHKYSSKMVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNI 180
                      LFIHKYSSKMVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNI
Sbjct: 121 -----------LFIHKYSSKMVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNI 180

Query: 181 GVGSFLIGIIGNFYHHYLLSQTRKQGDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFIS 240
           GVGSFLIGIIGNFYHHYLLSQTRKQGDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFIS
Sbjct: 181 GVGSFLIGIIGNFYHHYLLSQTRKQGDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFIS 240

Query: 241 QELYPLCFSIGTAVYLAGRSYATRKWYISKFEAFPNNVKALLPFVF 286
           QELYPLCFSIGTAVYLAGRSYATRKWYISKFEAFPNNVKALLPFVF
Sbjct: 241 QELYPLCFSIGTAVYLAGRSYATRKWYISKFEAFPNNVKALLPFVF 268

[Close]

BLAST of CsGy5G025485 vs. ExPASy TrEMBL
Match: A0A1S3BFS0 (3-oxo-5-alpha-steroid 4-dehydrogenase 2-like OS=Cucumis melo OX=3656 GN=LOC103489360 PE=3 SV=1)

HSP 1 Score: 510 bits (1313), Expect = 1.19e-181
Identity = 253/274 (92.34%), Postives = 263/274 (95.99%), Query Frame = 0


Query: 13  FPIWVSAAMDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNANGA 72
           FPIWVS AMD+LSAFLFPPPPSTFVNVMTVVS+T+LAMAGLSEI GKHLQYSKFWNAN  
Sbjct: 3   FPIWVSVAMDILSAFLFPPPPSTFVNVMTVVSSTSLAMAGLSEIKGKHLQYSKFWNANSG 62

Query: 73  SKLSAEESKVSSKYGMLLAYTPAFLAGAASFWLFPSGDERVFLLKSALTLHFFKRIFEVL 132
           SKLSA+ESK+SSKYGMLL YTPAFLAGAASFWLFPSGDER FLLKSALTLHFFKRIFEVL
Sbjct: 63  SKLSAQESKLSSKYGMLLVYTPAFLAGAASFWLFPSGDERFFLLKSALTLHFFKRIFEVL 122

Query: 133 FIHKYSSKMVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSFLIGIIGN 192
           FIHKYSSKMVLKTAIQIS SYF+STAIMIYTQHLSQGLPEPPIDLKNIGVG FL GIIGN
Sbjct: 123 FIHKYSSKMVLKTAIQISFSYFLSTAIMIYTQHLSQGLPEPPIDLKNIGVGLFLTGIIGN 182

Query: 193 FYHHYLLSQTRKQGDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFSIGT 252
           FYHHYLLSQTRKQ +TAYKIPKGGLFSFV+CPHYLFEIIGYFGFAFISQELYPLCFSIGT
Sbjct: 183 FYHHYLLSQTRKQEETAYKIPKGGLFSFVICPHYLFEIIGYFGFAFISQELYPLCFSIGT 242

Query: 253 AVYLAGRSYATRKWYISKFEAFPNNVKALLPFVF 286
           A+YLAGRSYATRKWYISKFE FPN+VKALLPFVF
Sbjct: 243 AIYLAGRSYATRKWYISKFENFPNHVKALLPFVF 276

[Close]

BLAST of CsGy5G025485 vs. ExPASy TrEMBL
Match: A0A6J1GZA3 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111458588 PE=3 SV=1)

HSP 1 Score: 452 bits (1163), Expect = 5.91e-159
Identity = 220/266 (82.71%), Postives = 243/266 (91.35%), Query Frame = 0


Query: 21  MDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNANGASKLSAEES 80
           M +LSAFLFPPPPSTFVNVMTVVS+TA+ MAG+SEINGKHLQYSKFWNAN + K  +EE 
Sbjct: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60

Query: 81  KVSSKYGMLLAYTPAFLAGAASFWLFPSGDERVFLLKSALTLHFFKRIFEVLFIHKYSSK 140
           K+SSKYGMLLAYTPAFLAGAASFW FPS +ER+ +LK+ALTLHFFKRIFEVLFIHKYSSK
Sbjct: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120

Query: 141 MVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSFLIGIIGNFYHHYLLS 200
           MV  TAI+IS SY +ST IMIYTQHLSQGLPEPPIDLKNIG+  FLIGIIGNFYHH+LLS
Sbjct: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGLPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180

Query: 201 QTRKQGDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFSIGTAVYLAGRS 260
           +TRK G+T YKIPKGGLFS V+CPHYLFEII +FGFAFISQELYPLCFS GTA+YLAGRS
Sbjct: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240

Query: 261 YATRKWYISKFEAFPNNVKALLPFVF 286
           YATRKWY+SKFE FP++VKALLPFVF
Sbjct: 241 YATRKWYVSKFENFPSHVKALLPFVF 266

[Close]

BLAST of CsGy5G025485 vs. ExPASy TrEMBL
Match: A0A6J1J6R3 (3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111483043 PE=3 SV=1)

HSP 1 Score: 451 bits (1161), Expect = 1.19e-158
Identity = 219/266 (82.33%), Postives = 243/266 (91.35%), Query Frame = 0


Query: 21  MDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNANGASKLSAEES 80
           M +LSAFLFPPPPSTFVNVMTVVS+TA+ MAG+SEINGKHLQYSKFWNAN + K  +EE 
Sbjct: 1   MGILSAFLFPPPPSTFVNVMTVVSSTAMGMAGISEINGKHLQYSKFWNANSSPKAPSEEP 60

Query: 81  KVSSKYGMLLAYTPAFLAGAASFWLFPSGDERVFLLKSALTLHFFKRIFEVLFIHKYSSK 140
           K+SSKYGMLLAYTPAFLAGAASFW FPS +ER+ +LK+ALTLHFFKRIFEVLFIHKYSSK
Sbjct: 61  KLSSKYGMLLAYTPAFLAGAASFWFFPSDNERILVLKAALTLHFFKRIFEVLFIHKYSSK 120

Query: 141 MVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSFLIGIIGNFYHHYLLS 200
           MV  TAI+IS SY +ST IMIYTQHLSQG+PEPPIDLKNIG+  FLIGIIGNFYHH+LLS
Sbjct: 121 MVFDTAIKISFSYCLSTVIMIYTQHLSQGIPEPPIDLKNIGIALFLIGIIGNFYHHFLLS 180

Query: 201 QTRKQGDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFSIGTAVYLAGRS 260
           +TRK G+T YKIPKGGLFS V+CPHYLFEII +FGFAFISQELYPLCFS GTA+YLAGRS
Sbjct: 181 KTRKPGETGYKIPKGGLFSLVICPHYLFEIIEFFGFAFISQELYPLCFSTGTAIYLAGRS 240

Query: 261 YATRKWYISKFEAFPNNVKALLPFVF 286
           YATRKWY+SKFE FP++VKALLPFVF
Sbjct: 241 YATRKWYVSKFENFPSHVKALLPFVF 266

[Close]

BLAST of CsGy5G025485 vs. ExPASy TrEMBL
Match: A0A6J1DAI6 (very-long-chain enoyl-CoA reductase-like OS=Momordica charantia OX=3673 GN=LOC111018692 PE=3 SV=1)

HSP 1 Score: 449 bits (1154), Expect = 1.39e-157
Identity = 218/266 (81.95%), Postives = 242/266 (90.98%), Query Frame = 0


Query: 21  MDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNANGASKLSAEES 80
           MD+ SAFLFPPPPSTFVNVMTVVS+TA+AM+G SE+NGKHLQYSKFWNANGASK S+EES
Sbjct: 1   MDISSAFLFPPPPSTFVNVMTVVSSTAMAMSGFSEMNGKHLQYSKFWNANGASKSSSEES 60

Query: 81  KVSSKYGMLLAYTPAFLAGAASFWLFPSGDERVFLLKSALTLHFFKRIFEVLFIHKYSSK 140
           KVSSKYGMLLAYTPAFLAGAASFW FPS D R+ LLKSALT HFFKRIFEVLF+HKYSSK
Sbjct: 61  KVSSKYGMLLAYTPAFLAGAASFWFFPSDDGRILLLKSALTFHFFKRIFEVLFVHKYSSK 120

Query: 141 MVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSFLIGIIGNFYHHYLLS 200
           MVL TA +ISLSYF+STAIMIYTQHLSQG P P IDLKNIG+G F++GIIGNFYHHYLLS
Sbjct: 121 MVLDTAFKISLSYFLSTAIMIYTQHLSQGFPAPSIDLKNIGIGCFVMGIIGNFYHHYLLS 180

Query: 201 QTRKQGDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFSIGTAVYLAGRS 260
           +TR +G+  YKIP GGLF  V+CPHYLFEII YFGFAFISQ ++PLCF+ GTA+YLAGRS
Sbjct: 181 RTRGKGEKEYKIPNGGLFCLVICPHYLFEIIVYFGFAFISQAIFPLCFATGTALYLAGRS 240

Query: 261 YATRKWYISKFEAFPNNVKALLPFVF 286
           YATRKWY+SKFE FPN+VKALLPF+F
Sbjct: 241 YATRKWYVSKFEHFPNHVKALLPFLF 266

[Close]

BLAST of CsGy5G025485 vs. TAIR 10
Match: AT5G16010.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 305.8 bits (782), Expect = 3.6e-83
Identity = 151/268 (56.34%), Postives = 200/268 (74.63%), Query Frame = 0


Query: 21  MDVLSAFLFPPPPSTFVNVMTVVSTTALAMAGLSEINGKHLQYSKFWNANGASKLSAEE- 80
           M+++++F++PPPPS  +N M+VV   ALA  G SEI G HL+YSKF  ++ + +   E  
Sbjct: 1   MEMVTSFVYPPPPSILLNCMSVVGVAALANIGWSEIRGNHLKYSKFGVSSSSPQPQKERF 60

Query: 81  SKVSSKYGMLLAYTPAFLAGAASFWLFPSGDERVFLLKSALTLHFFKRIFEVLFIHKYSS 140
             +SS+ GMLL YTPAFLA A+SF++ PS D R  LLKSAL LHFFKR+FEVLFIHKYS 
Sbjct: 61  GSISSRNGMLLLYTPAFLAAASSFFVVPSDDLRFLLLKSALALHFFKRVFEVLFIHKYSG 120

Query: 141 KMVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLKNIGVGSFLIGIIGNFYHHYLL 200
            M + +A+ IS SYF STA+M+Y+Q+L+ GL EP  D+K  GV  F++GI+GN YHH LL
Sbjct: 121 GMAIDSALTISSSYFSSTALMLYSQNLTLGLTEPSFDMKLAGVVMFVVGIVGNLYHHVLL 180

Query: 201 SQTRKQ-GDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFISQELYPLCFSIGTAVYLAG 260
           ++ RK+ G   YKIPKGGLF  ++CPHYLFEI+ ++ F  ISQ +Y   F++GT +YL G
Sbjct: 181 AKLRKEDGKKEYKIPKGGLFDIIICPHYLFEILVFWSFFLISQTIYSFSFAMGTMLYLIG 240

Query: 261 RSYATRKWYISKFEAFPNNVKALLPFVF 287
           RSYATR WY+SKF+ FP ++KAL+PFVF
Sbjct: 241 RSYATRTWYLSKFDDFPKHIKALIPFVF 268

[Close]

BLAST of CsGy5G025485 vs. TAIR 10
Match: AT2G38050.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 71.6 bits (174), Expect = 1.1e-12
Identity = 39/108 (36.11%), Postives = 60/108 (55.56%), Query Frame = 0


Query: 180 IGVGSFLIGIIGNFYHHYLLSQTRKQGDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFI 239
           IG+  F+ G+  N      L + +K+    Y IP+GG F  V CP+Y  E I + G+A +
Sbjct: 155 IGMVVFITGMYINITSDRTLVRLKKENRGGYVIPRGGWFELVSCPNYFGEAIEWLGWAVM 214

Query: 240 SQELYPLCFSIGTAVYLAGRSYATRKWYISKF-EAFPNNVKALLPFVF 287
           +     + F + T   L  R+ A+ KWYI+KF E +P   KA++PFV+
Sbjct: 215 TWSWAGIGFFLYTCSNLFPRARASHKWYIAKFKEEYPKTRKAVIPFVY 262

[Close]

BLAST of CsGy5G025485 vs. TAIR 10
Match: AT3G55360.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 67.0 bits (162), Expect = 2.8e-11
Identity = 48/150 (32.00%), Postives = 72/150 (48.00%), Query Frame = 0


Query: 123 HFFKRIFEVLFIHKYSSKMVLKTAIQISLSYFMSTAIMIYTQHLSQGLPEPPIDLK-NIG 182
           H+FKRI E  F+H++S        +  + +Y+ S    I   +++  L  P  DL+  IG
Sbjct: 141 HYFKRILETFFVHRFSHATSPIGNVFRNCAYYWSFGAYI-AYYVNHPLYTPVSDLQMKIG 200

Query: 183 VGSFLIGIIGNFYHHYLLSQTR-KQGDTAYKIPKGGLFSFVVCPHYLFEIIGYFGFAFIS 242
            G  L+  + NFY H LL   R   G   Y+IP+G LF+ V C +Y  EI  + GF   +
Sbjct: 201 FGFGLVCQVANFYCHILLKNLRDPSGAGGYQIPRGFLFNIVTCANYTTEIYQWLGFNIAT 260

Query: 243 QELYPLCFSIGTAVYLAGRSYATRKWYISK 271
           Q       +I   V+LA  +     W + K
Sbjct: 261 Q-------TIAGYVFLAVAALIMTNWALGK 282

[Close]

BLAST of CsGy5G025485 vs. TAIR 10
Match: AT1G72590.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 56.6 bits (135), Expect = 3.8e-08
Identity = 41/117 (35.04%), Postives = 54/117 (46.15%), Query Frame = 0


Query: 177 LKNIGVGSFLIGIIGNFYHHYLLSQTRKQGDTA--YKIPKGGLFSFVVCPHYLFEIIGYF 236
           L+ IG   FL G I     H +L   R+    A  Y IP G  F  V CPH+L EI+ Y 
Sbjct: 207 LQWIGGAIFLWGWIHQRRCHAILGSLREYPSQAKEYIIPYGDWFEMVSCPHFLAEIVLYL 266

Query: 237 GFAFISQ----ELYPLC-FSIGTAVYLAGRSYATRKWYISKFEAFPNNVKALLPFVF 287
           G    S      ++ L  F      Y AG    T +WY+ KFE +P +  A+ P V+
Sbjct: 267 GLLISSGGTDISIWLLFGFVAANLTYAAGE---THRWYLQKFENYPASRHAIFPHVY 320

[Close]

BLAST of CsGy5G025485 vs. TAIR 10
Match: AT2G16530.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 55.5 bits (132), Expect = 8.4e-08
Identity = 41/117 (35.04%), Postives = 54/117 (46.15%), Query Frame = 0


Query: 177 LKNIGVGSFLIGIIGNFYHHYLLSQTRKQGDTA--YKIPKGGLFSFVVCPHYLFEIIGYF 236
           L+ IG   FL G I     H +L   R+    A  Y IP G  F  V  PH+L EI+ Y 
Sbjct: 230 LQWIGGAIFLWGWIHQRRCHAILGSLRENPSQAKEYIIPYGDWFGMVSSPHFLAEIVLYA 289

Query: 237 GFAFISQ----ELYPLC-FSIGTAVYLAGRSYATRKWYISKFEAFPNNVKALLPFVF 287
           G    S      ++ L  F      Y AG    T +WY+ KFE +P N  A+ P+V+
Sbjct: 290 GLLIASGGTDITIWLLFGFVAANLTYAAGE---THRWYLRKFENYPANRHAIFPYVY 343

The following BLAST results are available for this feature:

BLAST of CsGy5G025485 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q28891	3.9e-18	40.19	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Macaca fascicularis OX=9541 GN=SRD5A1...	[more]
P18405	1.1e-17	39.25	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Homo sapiens OX=9606 GN=SRD5A1 PE=1 S...	[more]
Q55C17	5.7e-17	29.73	Very-long-chain enoyl-CoA reductase OS=Dictyostelium discoideum OX=44689 GN=gpsn...	[more]
Q2QDF6	9.7e-17	31.50	Steroid 5-alpha-reductase DET2 OS=Gossypium hirsutum OX=3635 GN=DET2 PE=1 SV=1	[more]
Q28892	2.8e-16	29.55	3-oxo-5-alpha-steroid 4-dehydrogenase 2 OS=Macaca fascicularis OX=9541 GN=SRD5A2...	[more]

BLAST of CsGy5G025485 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004135052.2	6.91e-204	100.00	3-oxo-5-alpha-steroid 4-dehydrogenase 2 [Cucumis sativus] >KAE8648808.1 hypothet...	[more]
XP_008446722.1	2.46e-181	92.34	PREDICTED: 3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Cucumis melo]	[more]
XP_038891781.1	5.81e-174	87.19	3-oxo-5-alpha-steroid 4-dehydrogenase 2-like [Benincasa hispida]	[more]
XP_022957108.1	1.22e-158	82.71	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita moschata]	[more]
XP_022984951.1	2.46e-158	82.33	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like [Cucurbita maxima]	[more]

BLAST of CsGy5G025485 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KTC7	7.11e-188	93.71	S5A_REDUCTASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G6128...	[more]
A0A1S3BFS0	1.19e-181	92.34	3-oxo-5-alpha-steroid 4-dehydrogenase 2-like OS=Cucumis melo OX=3656 GN=LOC10348...	[more]
A0A6J1GZA3	5.91e-159	82.71	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A6J1J6R3	1.19e-158	82.33	3-oxo-5-alpha-steroid 4-dehydrogenase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A6J1DAI6	1.39e-157	81.95	very-long-chain enoyl-CoA reductase-like OS=Momordica charantia OX=3673 GN=LOC11...	[more]

BLAST of CsGy5G025485 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G16010.1	3.6e-83	56.34	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G38050.1	1.1e-12	36.11	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT3G55360.1	2.8e-11	32.00	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT1G72590.1	3.8e-08	35.04	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G16530.1	8.4e-08	35.04	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PFAM	PF02544	Steroid_dh	coord: 177..286 e-value: 7.6E-28 score: 97.4
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PROSITE	PS50244	S5A_REDUCTASE	coord: 181..261 score: 24.887083
None	No IPR available	GENE3D	1.20.120.1630		coord: 112..285 e-value: 2.3E-6 score: 29.5
None	No IPR available	PANTHER	PTHR10556:SF35	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE FAMILY PROTEIN	coord: 26..286
IPR039357	3-oxo-5-alpha-steroid 4-dehydrogenase/very-long-chain enoyl-CoA reductase	PANTHER	PTHR10556	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE	coord: 26..286

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsGy5G025485.1	CsGy5G025485.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors