CsGy5G022580 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy5G022580
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionPyruvate dehydrogenase E1 component subunit alpha
LocationGy14Chr5: 27920423 .. 27923790 (-)
RNA-Seq ExpressionCsGy5G022580
SyntenyCsGy5G022580
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTATTGGATTTGTGTTGTAGATTCTAAAATTGCGAAGTTAAAAGAATATGTGTATATTTCCTTTATCAAATAGAATAAAATGAAGTTGTAGAGAGAGAGAGAGAGAGACATTCAACGACTCATCACTTCTTGAACAGCCAACTTCCATTTTTCTTTCCCTATCTTTCCTTTTTTTCTTTATCCTTCACTTCTTTTTAATATTCCCGCCTCCCGCCGGTCACCGCCATTTCCCTACGCTCACTCTACCGCCCTCCTCATGTCTCATTCTCGCATTTCTTCTTCCTCCATTCTCAAACCTCTCTCTTCTTCTTTCCGTTTCCTTTCGTCTGCCTCCCACCTCACAATCCAAACTTCTGTCCCCTTCACTGCTCACAAATGCGAGCCACCATCGCGTTCCGTGGACACCAGTCCCAACGAGTTACTTGCTTTCTTTCGCGAAATGGCACTTATGCGACGCATGGAAATCGCCGCCGACTCGCTTTATAAAGCCAAATTGATTCGAGGATTTTGCCATCTCTACGACGGTCAGGAAGCCGTGGCTGTTGGAATGGAGGCTGCAATCACCAAGAAGGACGCCATTATCACCGCTTATCGGGATCACTGCATCTTCCTCGGCCGTGGGGGAACGCTTCTTCAGTCATTCGCCGAACTCATGGGCCGTCAAGCTGGGTGCTCTAAAGGAAAAGGGGGTTCGATGCATTTTTATAAGAAGGACGCTGGATTCTATGGCGGCCATGGAATAGTGGGGGCTCAAGTGCCTTTGGGATGTGGTTTGGCTTTTGCTCAGAAGTACTCGAAGGAGGGTACCGTGACCTTTGCTCTTTACGGTGACGGTGCGGCAAATCAGGGCCAGTTGTTCGAGGCGCTCAACATATCTGCTCTTTGGGACTTGCCGGTAATTTTGGTCTGTGAGAATAATCATTGTAAGTAATTGTTAGTTCTTGTCAGTTTAATCAGTATTTGTGCTTCTATAATCTGCTATAACAATTGATAATGTATAAATACTTCTTAGCATCGCGATATGCAATATGAAACATGAAATTAGGTTAGTTTATATCTATAGAACGTGTGTAATCTCTTGAGTATCTAGTGTTTCAAAGGATCCAGTACAAGCATGTTTATGCTATTTACGATTTTTACCATAGGTTCTATTGAAGTGTGTTATATATTACTCTGTAATTCTGATCTTATAATAAACTATTCTTACATATGGAAGTAGCTAGCACATTAGTGAACCTCTTAAATCTCTCTGTTAATTTTCTATTGCTTTATGTTTTCTGATTTCTTCCTCTGTTAATTCCGTAACAGAATGAACAAACACATCGATTAGTGAGCTAATATTGATTGTCTTTTGTTGATGCAGATGGTATGGGAACTGCAGAGTGGAGAGCAGCCAAGAGTGCAGCCTATTATAAGCGTGGTGACTATGTTCCTGGACTGAAGGTATCATTTTCATTTTAATTAGATGAGAAGTGGGAGTTATAGTTCCTAATGTTACAGATTTTCTACTATGCTTATGATCTTTCCTTTCTTTTCCCCTTATTGAATTATTCAACATTAATTGAATTAGTCGATTCATTATTGTTCTTGAATTCGTCTTGTACTGTTGTTAAAACTTAGCTAGTTTGATGTTTATATTGTTTCCTTAAACAGGCAACTATTTCTTGTTTTTTGTTATAGGTGGATGGCATGGATGCCCTTGCTGTCAAGCAAGCTTGTAAGTTTGCTAAGGAGCATGCCTTAAAGAATGGACCCATTGTAAGTTTATCTTTCTTAGATAAATATTATTCAACTAAATACATTACGAGCTTAATTGCGCTGAACAAAACACGTGTATTGCATTTGTTTCAAGCGTATTCCTGTTTAGGAATTTGTTGTTTTTTTCAATTTGAATCAACCAATTTGGTATCTTTTCCACATTTATAGGAGTACGTTTGTGTTTCTACTTTACGAATATGATATTAATTTTCTGGGAATTTCTAATTTCTAGAGTTTTAGCCCTAAAAACACGTCACTGCCTAATAACTTTCTGAACTCCTGAACATGCCATACTTCTGCCCCGAGCCAATTGTTTCTGTGGCTTTGTTTCACTTTATTTTAGAAGCAGTTTCTCCCTGTATAAAAACCTCACTTGATTTTCGAAGTAAACACTATTGGATTTCTGCGGTGATGTATAAAGCTTAGTCTGTTGAGCAATGCTTTCCTTTGTTTTCCTTCCACTTGGAATTTAGTTTATCTCTCTTGACTTTCAATTTCAGATTCTCGAGATGGACACTTACAGGTACCATGGTCATTCTATGTCTGATCCTGGAAGTACCTACCGTACACGTGATGAGATTAGCGGTGTGAGACAGGTCTGATCAGCTTCCTTAATGAACTCATCCCAGCTTCATAACTTCTTCGCTCTGATTTCTAAATAAATTTTCTGATTGATCAATCAGGAACGTGATCCAATTGAAAGGATTAGAAAACTGTTATTATCTCATGATTTAGCCACCGAAAAGGATCTGAAGGTATCTTTTAACTTCTCAATGATGCGTTGATGTTTTAAATTTAGTTCCAAAGAGGGAAAAAAAATTGTTTTTGTGTCTTTTGATCATCAGGATATTGAGAAGGAAATGAGAAAAGAAGTAGATGAAGCCATTGCTCAAGCCAAGGTAAGACGTGCAATTATCATCTAGCAAACTTATGATTTTCGAACAACTATATGGTTAACAACCTTAACCCGTAGTAATCCTGCTTTCTTCATCGCTATCTCGTTTGTGTTTCCAGGCAAGTCCAATGCCAGATTTGTCAGAACTTTTCTCAAACGTGTACGTGAAAGGATTTGAAGCTGAGGTAATGTATTACATCTCCCATCAACAGAGATAATGAAAAGGAAACCAAAGTCATTAGAATGTAATCTAAATACCCAGACGGTTTTTTCGTGCGTCTTTACAGGTGTTCGGAGCAGACCGAAAAGAAGTCAGGGGTGTTCTTCCATAAGTAAGACAAAGAGTTGGCATTGTATAGACGACCTTTTCACACGAATGCAGTATACCAACGAAACCAAATCCTTATGGTGAAAAAATCCAAAATAACAAACATGATTTCCTTTTTTCTGAATTTGATAGAAACAGCTCTCTTTTTCATGTTCATGGAAACCACTCTGTGGTTGAAATATAAGTTTGCTCTTTTAAGGTTTCTGAATAGCTAGTAAAAGGAAGAAAAAAAACCCTCCTGTATTAATACCTTGAATCACTTATTCCTTAAGTTACAATACTTGTACATAATCAAAATAAAGTTCTTCATGGGGAGAATTGCCCAATTATTTTATTATGCACTACAATGGATTTTAATTTAATTTTTAAACTTTCCTTTGATCATAG

mRNA sequence

CTATTGGATTTGTGTTGTAGATTCTAAAATTGCGAAGTTAAAAGAATATGTGTATATTTCCTTTATCAAATAGAATAAAATGAAGTTGTAGAGAGAGAGAGAGAGAGACATTCAACGACTCATCACTTCTTGAACAGCCAACTTCCATTTTTCTTTCCCTATCTTTCCTTTTTTTCTTTATCCTTCACTTCTTTTTAATATTCCCGCCTCCCGCCGGTCACCGCCATTTCCCTACGCTCACTCTACCGCCCTCCTCATGTCTCATTCTCGCATTTCTTCTTCCTCCATTCTCAAACCTCTCTCTTCTTCTTTCCGTTTCCTTTCGTCTGCCTCCCACCTCACAATCCAAACTTCTGTCCCCTTCACTGCTCACAAATGCGAGCCACCATCGCGTTCCGTGGACACCAGTCCCAACGAGTTACTTGCTTTCTTTCGCGAAATGGCACTTATGCGACGCATGGAAATCGCCGCCGACTCGCTTTATAAAGCCAAATTGATTCGAGGATTTTGCCATCTCTACGACGGTCAGGAAGCCGTGGCTGTTGGAATGGAGGCTGCAATCACCAAGAAGGACGCCATTATCACCGCTTATCGGGATCACTGCATCTTCCTCGGCCGTGGGGGAACGCTTCTTCAGTCATTCGCCGAACTCATGGGCCGTCAAGCTGGGTGCTCTAAAGGAAAAGGGGGTTCGATGCATTTTTATAAGAAGGACGCTGGATTCTATGGCGGCCATGGAATAGTGGGGGCTCAAGTGCCTTTGGGATGTGGTTTGGCTTTTGCTCAGAAGTACTCGAAGGAGGGTACCGTGACCTTTGCTCTTTACGGTGACGGTGCGGCAAATCAGGGCCAGTTGTTCGAGGCGCTCAACATATCTGCTCTTTGGGACTTGCCGGTAATTTTGGTCTGTGAGAATAATCATTATGGTATGGGAACTGCAGAGTGGAGAGCAGCCAAGAGTGCAGCCTATTATAAGCGTGGTGACTATGTTCCTGGACTGAAGGTGGATGGCATGGATGCCCTTGCTGTCAAGCAAGCTTGTAAGTTTGCTAAGGAGCATGCCTTAAAGAATGGACCCATTATTCTCGAGATGGACACTTACAGGTACCATGGTCATTCTATGTCTGATCCTGGAAGTACCTACCGTACACGTGATGAGATTAGCGGTGTGAGACAGGAACGTGATCCAATTGAAAGGATTAGAAAACTGTTATTATCTCATGATTTAGCCACCGAAAAGGATCTGAAGGATATTGAGAAGGAAATGAGAAAAGAAGTAGATGAAGCCATTGCTCAAGCCAAGGCAAGTCCAATGCCAGATTTGTCAGAACTTTTCTCAAACGTGTACGTGAAAGGATTTGAAGCTGAGGTGTTCGGAGCAGACCGAAAAGAAGTCAGGGGTGTTCTTCCATAAGTAAGACAAAGAGTTGGCATTGTATAGACGACCTTTTCACACGAATGCAGTATACCAACGAAACCAAATCCTTATGGTGAAAAAATCCAAAATAACAAACATGATTTCCTTTTTTCTGAATTTGATAGAAACAGCTCTCTTTTTCATGTTCATGGAAACCACTCTGTGGTTGAAATATAAGTTTGCTCTTTTAAGGTTTCTGAATAGCTAGTAAAAGGAAGAAAAAAAACCCTCCTGTATTAATACCTTGAATCACTTATTCCTTAAGTTACAATACTTGTACATAATCAAAATAAAGTTCTTCATGGGGAGAATTGCCCAATTATTTTATTATGCACTACAATGGATTTTAATTTAATTTTTAAACTTTCCTTTGATCATAG

Coding sequence (CDS)

ATGTCTCATTCTCGCATTTCTTCTTCCTCCATTCTCAAACCTCTCTCTTCTTCTTTCCGTTTCCTTTCGTCTGCCTCCCACCTCACAATCCAAACTTCTGTCCCCTTCACTGCTCACAAATGCGAGCCACCATCGCGTTCCGTGGACACCAGTCCCAACGAGTTACTTGCTTTCTTTCGCGAAATGGCACTTATGCGACGCATGGAAATCGCCGCCGACTCGCTTTATAAAGCCAAATTGATTCGAGGATTTTGCCATCTCTACGACGGTCAGGAAGCCGTGGCTGTTGGAATGGAGGCTGCAATCACCAAGAAGGACGCCATTATCACCGCTTATCGGGATCACTGCATCTTCCTCGGCCGTGGGGGAACGCTTCTTCAGTCATTCGCCGAACTCATGGGCCGTCAAGCTGGGTGCTCTAAAGGAAAAGGGGGTTCGATGCATTTTTATAAGAAGGACGCTGGATTCTATGGCGGCCATGGAATAGTGGGGGCTCAAGTGCCTTTGGGATGTGGTTTGGCTTTTGCTCAGAAGTACTCGAAGGAGGGTACCGTGACCTTTGCTCTTTACGGTGACGGTGCGGCAAATCAGGGCCAGTTGTTCGAGGCGCTCAACATATCTGCTCTTTGGGACTTGCCGGTAATTTTGGTCTGTGAGAATAATCATTATGGTATGGGAACTGCAGAGTGGAGAGCAGCCAAGAGTGCAGCCTATTATAAGCGTGGTGACTATGTTCCTGGACTGAAGGTGGATGGCATGGATGCCCTTGCTGTCAAGCAAGCTTGTAAGTTTGCTAAGGAGCATGCCTTAAAGAATGGACCCATTATTCTCGAGATGGACACTTACAGGTACCATGGTCATTCTATGTCTGATCCTGGAAGTACCTACCGTACACGTGATGAGATTAGCGGTGTGAGACAGGAACGTGATCCAATTGAAAGGATTAGAAAACTGTTATTATCTCATGATTTAGCCACCGAAAAGGATCTGAAGGATATTGAGAAGGAAATGAGAAAAGAAGTAGATGAAGCCATTGCTCAAGCCAAGGCAAGTCCAATGCCAGATTTGTCAGAACTTTTCTCAAACGTGTACGTGAAAGGATTTGAAGCTGAGGTGTTCGGAGCAGACCGAAAAGAAGTCAGGGGTGTTCTTCCATAA

Protein sequence

MSHSRISSSSILKPLSSSFRFLSSASHLTIQTSVPFTAHKCEPPSRSVDTSPNELLAFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHCIFLGRGGTLLQSFAELMGRQAGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFAQKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSAAYYKRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTYRTRDEISGVRQERDPIERIRKLLLSHDLATEKDLKDIEKEMRKEVDEAIAQAKASPMPDLSELFSNVYVKGFEAEVFGADRKEVRGVLP*
Homology
BLAST of CsGy5G022580 vs. ExPASy Swiss-Prot
Match: P52902 (Pyruvate dehydrogenase E1 component subunit alpha, mitochondrial OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 648.3 bits (1671), Expect = 5.6e-185
Identity = 326/397 (82.12%), Postives = 353/397 (88.92%), Query Frame = 0

Query: 1   MSHSRISSSS-------ILKPLSSSF---RFLSS--ASHLTIQTSVPFTAHKCEPPSRSV 60
           M+ SR+SSSS       +  P S++F   R +SS   + LTI+TS+PFTAH C+PPSRSV
Sbjct: 1   MALSRLSSSSSSSNGSNLFNPFSAAFTLNRPISSDTTATLTIETSLPFTAHNCDPPSRSV 60

Query: 61  DTSPNELLAFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAI 120
            TSP+ELL+FFR MALMRRMEIAADSLYKA LIRGFCHLYDGQEAVAVGMEA  TKKD I
Sbjct: 61  TTSPSELLSFFRTMALMRRMEIAADSLYKANLIRGFCHLYDGQEAVAVGMEAGTTKKDCI 120

Query: 121 ITAYRDHCIFLGRGGTLLQSFAELMGRQAGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVP 180
           ITAYRDHC FLGRGGTLL+ +AELMGR+ GCSKGKGGSMHFYKKD+GFYGGHGIVGAQVP
Sbjct: 121 ITAYRDHCTFLGRGGTLLRVYAELMGRRDGCSKGKGGSMHFYKKDSGFYGGHGIVGAQVP 180

Query: 181 LGCGLAFAQKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTA 240
           LGCGLAF QKY K+ +VTFALYGDGAANQGQLFEALNISALWDLP ILVCENNHYGMGTA
Sbjct: 181 LGCGLAFGQKYLKDESVTFALYGDGAANQGQLFEALNISALWDLPAILVCENNHYGMGTA 240

Query: 241 EWRAAKSAAYYKRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHS 300
            WR+AKS AY+KRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHS
Sbjct: 241 TWRSAKSPAYFKRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHS 300

Query: 301 MSDPGSTYRTRDEISGVRQERDPIERIRKLLLSHDLATEKDLKDIEKEMRKEVDEAIAQA 360
           MSDPGSTYRTRDEISGVRQERDPIER+RKLLLSHD+ATEK+LKD EKE+RKEVDEAIA+A
Sbjct: 301 MSDPGSTYRTRDEISGVRQERDPIERVRKLLLSHDIATEKELKDTEKEVRKEVDEAIAKA 360

Query: 361 KASPMPDLSELFSNVYVKGFEAEVFGADRKEVRGVLP 386
           K SPMPD S+LFSNVYVKG+  E FG DRKEVR  LP
Sbjct: 361 KDSPMPDPSDLFSNVYVKGYGVEAFGVDRKEVRVTLP 397

BLAST of CsGy5G022580 vs. ExPASy Swiss-Prot
Match: P52901 (Pyruvate dehydrogenase E1 component subunit alpha-1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=E1 ALPHA PE=1 SV=2)

HSP 1 Score: 643.7 bits (1659), Expect = 1.4e-183
Identity = 320/389 (82.26%), Postives = 353/389 (90.75%), Query Frame = 0

Query: 1   MSHSRISSSS--ILKPLSSSFRFLSS--ASHLTIQTSVPFTAHKCEPPSRSVDTSPNELL 60
           M+ SR+SS S  I +P S++F  L S   + +TI+TS+PFTAH C+PPSRSV++S  ELL
Sbjct: 1   MALSRLSSRSNIITRPFSAAFSRLISTDTTPITIETSLPFTAHLCDPPSRSVESSSQELL 60

Query: 61  AFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC 120
            FFR MALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVA+GMEAAITKKDAIITAYRDHC
Sbjct: 61  DFFRTMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAIGMEAAITKKDAIITAYRDHC 120

Query: 121 IFLGRGGTLLQSFAELMGRQAGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA 180
           IFLGRGG+L + F+ELMGRQAGCSKGKGGSMHFYKK++ FYGGHGIVGAQVPLGCG+AFA
Sbjct: 121 IFLGRGGSLHEVFSELMGRQAGCSKGKGGSMHFYKKESSFYGGHGIVGAQVPLGCGIAFA 180

Query: 181 QKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSA 240
           QKY+KE  VTFALYGDGAANQGQLFEALNISALWDLP ILVCENNHYGMGTAEWRAAKS 
Sbjct: 181 QKYNKEEAVTFALYGDGAANQGQLFEALNISALWDLPAILVCENNHYGMGTAEWRAAKSP 240

Query: 241 AYYKRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY 300
           +YYKRGDYVPGLKVDGMDA AVKQACKFAK+HAL+ GPIILEMDTYRYHGHSMSDPGSTY
Sbjct: 241 SYYKRGDYVPGLKVDGMDAFAVKQACKFAKQHALEKGPIILEMDTYRYHGHSMSDPGSTY 300

Query: 301 RTRDEISGVRQERDPIERIRKLLLSHDLATEKDLKDIEKEMRKEVDEAIAQAKASPMPDL 360
           RTRDEISGVRQERDPIERI+KL+LSHDLATEK+LKD+EKE+RKEVD+AIA+AK  PMP+ 
Sbjct: 301 RTRDEISGVRQERDPIERIKKLVLSHDLATEKELKDMEKEIRKEVDDAIAKAKDCPMPEP 360

Query: 361 SELFSNVYVKGFEAEVFGADRKEVRGVLP 386
           SELF+NVYVKGF  E FG DRKEV+  LP
Sbjct: 361 SELFTNVYVKGFGTESFGPDRKEVKASLP 389

BLAST of CsGy5G022580 vs. ExPASy Swiss-Prot
Match: Q6Z5N4 (Pyruvate dehydrogenase E1 component subunit alpha-1, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0739600 PE=2 SV=1)

HSP 1 Score: 633.3 bits (1632), Expect = 1.9e-180
Identity = 305/362 (84.25%), Postives = 331/362 (91.44%), Query Frame = 0

Query: 24  SASHLTIQTSVPFTAHKCEPPSRSVDTSPNELLAFFREMALMRRMEIAADSLYKAKLIRG 83
           S + LTI+TSVPFT+H  +PPSR V T+P ELL FFR+M++MRRMEIAADSLYKAKLIRG
Sbjct: 29  STAPLTIETSVPFTSHIVDPPSRDVTTTPAELLTFFRDMSVMRRMEIAADSLYKAKLIRG 88

Query: 84  FCHLYDGQEAVAVGMEAAITKKDAIITAYRDHCIFLGRGGTLLQSFAELMGRQAGCSKGK 143
           FCHLYDGQEAVAVGMEAAIT+ D+IITAYRDHC +L RGG L+ +FAELMGRQAGCS+GK
Sbjct: 89  FCHLYDGQEAVAVGMEAAITRSDSIITAYRDHCTYLARGGDLVSAFAELMGRQAGCSRGK 148

Query: 144 GGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFAQKYSKEGTVTFALYGDGAANQGQLFEA 203
           GGSMHFYKKDA FYGGHGIVGAQVPLGCGLAFAQKY KE T TFALYGDGAANQGQLFEA
Sbjct: 149 GGSMHFYKKDANFYGGHGIVGAQVPLGCGLAFAQKYRKEETATFALYGDGAANQGQLFEA 208

Query: 204 LNISALWDLPVILVCENNHYGMGTAEWRAAKSAAYYKRGDYVPGLKVDGMDALAVKQACK 263
           LNISALW LP ILVCENNHYGMGTAEWRAAKS AYYKRGDYVPGLKVDGMD LAVKQACK
Sbjct: 209 LNISALWKLPAILVCENNHYGMGTAEWRAAKSPAYYKRGDYVPGLKVDGMDVLAVKQACK 268

Query: 264 FAKEHALKNGPIILEMDTYRYHGHSMSDPGSTYRTRDEISGVRQERDPIERIRKLLLSHD 323
           FAKEHA+ NGPI+LEMDTYRYHGHSMSDPGSTYRTRDEISGVRQERDPIER+RKL+L+HD
Sbjct: 269 FAKEHAIANGPIVLEMDTYRYHGHSMSDPGSTYRTRDEISGVRQERDPIERVRKLILAHD 328

Query: 324 LATEKDLKDIEKEMRKEVDEAIAQAKASPMPDLSELFSNVYVKGFEAEVFGADRKEVRGV 383
           LAT  +LKD+EKE+RKEVD+AIA+AK SPMPD SELF+NVYVKGF  E FGADRKE+R  
Sbjct: 329 LATAAELKDMEKEIRKEVDDAIAKAKESPMPDTSELFTNVYVKGFGVESFGADRKELRAT 388

Query: 384 LP 386
           LP
Sbjct: 389 LP 390

BLAST of CsGy5G022580 vs. ExPASy Swiss-Prot
Match: Q8H1Y0 (Pyruvate dehydrogenase E1 component subunit alpha-2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=IAR4 PE=1 SV=2)

HSP 1 Score: 630.6 bits (1625), Expect = 1.2e-179
Identity = 314/393 (79.90%), Postives = 350/393 (89.06%), Query Frame = 0

Query: 1   MSHSRISSSS--ILKP----LSSSFR--FLSSASHLTIQTSVPFTAHKCEPPSRSVDTSP 60
           M+ SR+SS S   LKP    L SS R    + +S +TI+T+VPFT+H CE PSRSV+TS 
Sbjct: 1   MALSRLSSRSNTFLKPAITALPSSIRRHVSTDSSPITIETAVPFTSHLCESPSRSVETSS 60

Query: 61  NELLAFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAY 120
            E+LAFFR+MA MRRMEIAADSLYKAKLIRGFCHLYDGQEA+AVGMEAAITKKDAIIT+Y
Sbjct: 61  EEILAFFRDMARMRRMEIAADSLYKAKLIRGFCHLYDGQEALAVGMEAAITKKDAIITSY 120

Query: 121 RDHCIFLGRGGTLLQSFAELMGRQAGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCG 180
           RDHC F+GRGG L+ +F+ELMGR+ GCS GKGGSMHFYKKDA FYGGHGIVGAQ+PLGCG
Sbjct: 121 RDHCTFIGRGGKLVDAFSELMGRKTGCSHGKGGSMHFYKKDASFYGGHGIVGAQIPLGCG 180

Query: 181 LAFAQKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRA 240
           LAFAQKY+K+  VTFALYGDGAANQGQLFEALNISALWDLP ILVCENNHYGMGTA WR+
Sbjct: 181 LAFAQKYNKDEAVTFALYGDGAANQGQLFEALNISALWDLPAILVCENNHYGMGTATWRS 240

Query: 241 AKSAAYYKRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDP 300
           AKS AY+KRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDP
Sbjct: 241 AKSPAYFKRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDP 300

Query: 301 GSTYRTRDEISGVRQERDPIERIRKLLLSHDLATEKDLKDIEKEMRKEVDEAIAQAKASP 360
           GSTYRTRDEISGVRQ RDPIER+RKLLL+HD+ATEK+LKD+EKE+RKEVD+A+AQAK SP
Sbjct: 301 GSTYRTRDEISGVRQVRDPIERVRKLLLTHDIATEKELKDMEKEIRKEVDDAVAQAKESP 360

Query: 361 MPDLSELFSNVYVKGFEAEVFGADRKEVRGVLP 386
           +PD SELF+N+YVK    E FGADRKE++  LP
Sbjct: 361 IPDASELFTNMYVKDCGVESFGADRKELKVTLP 393

BLAST of CsGy5G022580 vs. ExPASy Swiss-Prot
Match: P52903 (Pyruvate dehydrogenase E1 component subunit alpha, mitochondrial OS=Solanum tuberosum OX=4113 PE=1 SV=1)

HSP 1 Score: 630.6 bits (1625), Expect = 1.2e-179
Identity = 308/380 (81.05%), Postives = 343/380 (90.26%), Query Frame = 0

Query: 11  ILKPLSSSF---RFLSSAS--HLTIQTSVPFTAHKCEPPSRSVDTSPNELLAFFREMALM 70
           I+KPLS++    R LSS S   +T++TS+PFT+H  +PPSRSV+TSP EL+ FF++M  M
Sbjct: 12  IMKPLSAAVCATRRLSSDSTATITVETSLPFTSHNIDPPSRSVETSPKELMTFFKDMTEM 71

Query: 71  RRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHCIFLGRGGTL 130
           RRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKD IITAYRDHCIFLGRGGTL
Sbjct: 72  RRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDCIITAYRDHCIFLGRGGTL 131

Query: 131 LQSFAELMGRQAGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFAQKYSKEGTV 190
           +++FAELMGR+ GCS+GKGGSMHFYKK++GFYGGHGIVGAQVPLG GLAFAQKY KE  V
Sbjct: 132 VEAFAELMGRRDGCSRGKGGSMHFYKKESGFYGGHGIVGAQVPLGIGLAFAQKYKKEDYV 191

Query: 191 TFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSAAYYKRGDYV 250
           TFA+YGDGAANQGQLFEALN++ALWDLP ILVCENNHYGMGTAEWRAAKS AYYKRGDYV
Sbjct: 192 TFAMYGDGAANQGQLFEALNMAALWDLPAILVCENNHYGMGTAEWRAAKSPAYYKRGDYV 251

Query: 251 PGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTYRTRDEISGV 310
           PGL+VDGMD  AVKQAC FAK+HALKNGPIILEMDTYRYHGHSMSDPGSTYRTRDEISGV
Sbjct: 252 PGLRVDGMDVFAVKQACTFAKQHALKNGPIILEMDTYRYHGHSMSDPGSTYRTRDEISGV 311

Query: 311 RQERDPIERIRKLLLSHDLATEKDLKDIEKEMRKEVDEAIAQAKASPMPDLSELFSNVYV 370
           RQERDP+ERIR L+L+H++ATE +LKDIEKE RK VDEAIA+AK SPMPD SELF+NVYV
Sbjct: 312 RQERDPVERIRSLILAHNIATEAELKDIEKENRKVVDEAIAKAKESPMPDPSELFTNVYV 371

Query: 371 KGFEAEVFGADRKEVRGVLP 386
           KGF  E +GADRKE+R  LP
Sbjct: 372 KGFGVEAYGADRKELRATLP 391

BLAST of CsGy5G022580 vs. NCBI nr
Match: XP_004135218.1 (pyruvate dehydrogenase E1 component subunit alpha, mitochondrial [Cucumis sativus] >KGN51850.1 hypothetical protein Csa_009017 [Cucumis sativus])

HSP 1 Score: 762 bits (1967), Expect = 1.61e-277
Identity = 385/385 (100.00%), Postives = 385/385 (100.00%), Query Frame = 0

Query: 1   MSHSRISSSSILKPLSSSFRFLSSASHLTIQTSVPFTAHKCEPPSRSVDTSPNELLAFFR 60
           MSHSRISSSSILKPLSSSFRFLSSASHLTIQTSVPFTAHKCEPPSRSVDTSPNELLAFFR
Sbjct: 1   MSHSRISSSSILKPLSSSFRFLSSASHLTIQTSVPFTAHKCEPPSRSVDTSPNELLAFFR 60

Query: 61  EMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHCIFLG 120
           EMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHCIFLG
Sbjct: 61  EMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHCIFLG 120

Query: 121 RGGTLLQSFAELMGRQAGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFAQKYS 180
           RGGTLLQSFAELMGRQAGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFAQKYS
Sbjct: 121 RGGTLLQSFAELMGRQAGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFAQKYS 180

Query: 181 KEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSAAYYK 240
           KEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSAAYYK
Sbjct: 181 KEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSAAYYK 240

Query: 241 RGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTYRTRD 300
           RGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTYRTRD
Sbjct: 241 RGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTYRTRD 300

Query: 301 EISGVRQERDPIERIRKLLLSHDLATEKDLKDIEKEMRKEVDEAIAQAKASPMPDLSELF 360
           EISGVRQERDPIERIRKLLLSHDLATEKDLKDIEKEMRKEVDEAIAQAKASPMPDLSELF
Sbjct: 301 EISGVRQERDPIERIRKLLLSHDLATEKDLKDIEKEMRKEVDEAIAQAKASPMPDLSELF 360

Query: 361 SNVYVKGFEAEVFGADRKEVRGVLP 385
           SNVYVKGFEAEVFGADRKEVRGVLP
Sbjct: 361 SNVYVKGFEAEVFGADRKEVRGVLP 385

BLAST of CsGy5G022580 vs. NCBI nr
Match: XP_008446275.1 (PREDICTED: pyruvate dehydrogenase E1 component subunit alpha, mitochondrial [Cucumis melo] >KAA0034351.1 pyruvate dehydrogenase E1 component subunit alpha [Cucumis melo var. makuwa] >TYK15568.1 pyruvate dehydrogenase E1 component subunit alpha [Cucumis melo var. makuwa])

HSP 1 Score: 737 bits (1902), Expect = 1.51e-267
Identity = 372/389 (95.63%), Postives = 380/389 (97.69%), Query Frame = 0

Query: 1   MSHSRISSSSILKPLSSSFRFLSSASH----LTIQTSVPFTAHKCEPPSRSVDTSPNELL 60
           MSHS ISSSSILKPLSSSFRFLSSA+H    LTIQTSVPFTAHKCEPPSRSVDTSPNELL
Sbjct: 1   MSHSPISSSSILKPLSSSFRFLSSAAHDSAHLTIQTSVPFTAHKCEPPSRSVDTSPNELL 60

Query: 61  AFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC 120
           AFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC
Sbjct: 61  AFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC 120

Query: 121 IFLGRGGTLLQSFAELMGRQAGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA 180
            FLGRGGTLL++FAELMGRQ GCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA
Sbjct: 121 TFLGRGGTLLEAFAELMGRQVGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA 180

Query: 181 QKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSA 240
           QKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKS+
Sbjct: 181 QKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSS 240

Query: 241 AYYKRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY 300
           AYYKRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY
Sbjct: 241 AYYKRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY 300

Query: 301 RTRDEISGVRQERDPIERIRKLLLSHDLATEKDLKDIEKEMRKEVDEAIAQAKASPMPDL 360
           RTRDEISGVRQERDPIERIRKLLLSH++ATEKDLKDIEKEMRK+VDEAIAQAKASPMPD 
Sbjct: 301 RTRDEISGVRQERDPIERIRKLLLSHEIATEKDLKDIEKEMRKKVDEAIAQAKASPMPDA 360

Query: 361 SELFSNVYVKGFEAEVFGADRKEVRGVLP 385
           SELF+NVYVKGFE EVFGADRKEVRGVLP
Sbjct: 361 SELFTNVYVKGFETEVFGADRKEVRGVLP 389

BLAST of CsGy5G022580 vs. NCBI nr
Match: XP_038891336.1 (pyruvate dehydrogenase E1 component subunit alpha, mitochondrial [Benincasa hispida])

HSP 1 Score: 716 bits (1847), Expect = 3.65e-259
Identity = 363/389 (93.32%), Postives = 371/389 (95.37%), Query Frame = 0

Query: 1   MSHSRISSSSILKPLSSSFRFLSSASH----LTIQTSVPFTAHKCEPPSRSVDTSPNELL 60
           MS SRI SSS LKP SSSFR LSSA+     LTIQTSVPFTAHKCEPPSRSVDTSP ELL
Sbjct: 1   MSLSRIPSSSFLKPFSSSFRLLSSAADVSADLTIQTSVPFTAHKCEPPSRSVDTSPKELL 60

Query: 61  AFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC 120
            FFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC
Sbjct: 61  TFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC 120

Query: 121 IFLGRGGTLLQSFAELMGRQAGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA 180
            FLGRGGTLL++FAELMGRQAGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA
Sbjct: 121 TFLGRGGTLLEAFAELMGRQAGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA 180

Query: 181 QKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSA 240
           QKYSKEGTVTFALYGDGAANQGQLFEALN+SALWDLPVILVCENNHYGMGTAEWRAAKS 
Sbjct: 181 QKYSKEGTVTFALYGDGAANQGQLFEALNMSALWDLPVILVCENNHYGMGTAEWRAAKSP 240

Query: 241 AYYKRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY 300
           AYYKRGDYVPGLKVDGMDALAVKQACKFAK+HALKNGPIILEMDTYRYHGHSMSDPGSTY
Sbjct: 241 AYYKRGDYVPGLKVDGMDALAVKQACKFAKDHALKNGPIILEMDTYRYHGHSMSDPGSTY 300

Query: 301 RTRDEISGVRQERDPIERIRKLLLSHDLATEKDLKDIEKEMRKEVDEAIAQAKASPMPDL 360
           RTRDEISGVRQERDPIERIRKLLLSH++ATEKDLKDIEKEMRKEVDEAIAQAK SPMPD 
Sbjct: 301 RTRDEISGVRQERDPIERIRKLLLSHEIATEKDLKDIEKEMRKEVDEAIAQAKGSPMPDP 360

Query: 361 SELFSNVYVKGFEAEVFGADRKEVRGVLP 385
           SELFSNVYVKGF AE FGADRKE+RGVLP
Sbjct: 361 SELFSNVYVKGFGAESFGADRKEIRGVLP 389

BLAST of CsGy5G022580 vs. NCBI nr
Match: XP_022943269.1 (pyruvate dehydrogenase E1 component subunit alpha, mitochondrial-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 687 bits (1772), Expect = 9.76e-248
Identity = 348/389 (89.46%), Postives = 361/389 (92.80%), Query Frame = 0

Query: 1   MSHSRISSSSILKPLSSSFRFLSSA----SHLTIQTSVPFTAHKCEPPSRSVDTSPNELL 60
           MS  R  SSSILKP SSS+   SSA    S LTI+TSVPFTAHKCE PSRSV TSPNELL
Sbjct: 1   MSLYRTPSSSILKPFSSSYPLFSSAAGHYSDLTIETSVPFTAHKCETPSRSVHTSPNELL 60

Query: 61  AFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC 120
           AFFR+MALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC
Sbjct: 61  AFFRQMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC 120

Query: 121 IFLGRGGTLLQSFAELMGRQAGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA 180
            FL RGGTLL++ AELMGRQAGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA
Sbjct: 121 TFLRRGGTLLEALAELMGRQAGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA 180

Query: 181 QKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSA 240
           QKYSK+GTVTF LYGDGAANQGQLFEALNISALWDLPVI VCENNHYGMGTAEWRAAKS 
Sbjct: 181 QKYSKDGTVTFVLYGDGAANQGQLFEALNISALWDLPVIFVCENNHYGMGTAEWRAAKSP 240

Query: 241 AYYKRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY 300
           AYYKRGDYVPG+KVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY
Sbjct: 241 AYYKRGDYVPGIKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY 300

Query: 301 RTRDEISGVRQERDPIERIRKLLLSHDLATEKDLKDIEKEMRKEVDEAIAQAKASPMPDL 360
           RTRDEI+G+RQERDPI+RIRKLLLSH++ATEKDLKDIEKEMRKEVDEAIAQAK SPMPD 
Sbjct: 301 RTRDEITGIRQERDPIDRIRKLLLSHEIATEKDLKDIEKEMRKEVDEAIAQAKESPMPDP 360

Query: 361 SELFSNVYVKGFEAEVFGADRKEVRGVLP 385
           +ELFSNVYV GF AE FGADRKEV  VLP
Sbjct: 361 TELFSNVYVNGFGAEAFGADRKEVMAVLP 389

BLAST of CsGy5G022580 vs. NCBI nr
Match: XP_023548204.1 (pyruvate dehydrogenase E1 component subunit alpha, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 687 bits (1772), Expect = 9.76e-248
Identity = 348/389 (89.46%), Postives = 361/389 (92.80%), Query Frame = 0

Query: 1   MSHSRISSSSILKPLSSSFRFLSSA----SHLTIQTSVPFTAHKCEPPSRSVDTSPNELL 60
           MS  R  SSSILKP SSS+   SSA    S LTI+TSVPFTAHKCE PSRSV TSPNELL
Sbjct: 1   MSLYRAPSSSILKPFSSSYPLFSSAAAHYSDLTIETSVPFTAHKCETPSRSVHTSPNELL 60

Query: 61  AFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC 120
           AFFR+MALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC
Sbjct: 61  AFFRQMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC 120

Query: 121 IFLGRGGTLLQSFAELMGRQAGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA 180
            FL RGGTLL++ AELMGRQAGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA
Sbjct: 121 TFLRRGGTLLEALAELMGRQAGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA 180

Query: 181 QKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSA 240
           QKYSK+GTVTF LYGDGAANQGQLFEALNISALWDLPVI VCENNHYGMGTAEWRAAKS 
Sbjct: 181 QKYSKDGTVTFVLYGDGAANQGQLFEALNISALWDLPVIFVCENNHYGMGTAEWRAAKSP 240

Query: 241 AYYKRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY 300
           AYYKRGDYVPG+KVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY
Sbjct: 241 AYYKRGDYVPGIKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY 300

Query: 301 RTRDEISGVRQERDPIERIRKLLLSHDLATEKDLKDIEKEMRKEVDEAIAQAKASPMPDL 360
           RTRDEI+G+RQERDPI+RIRKLLLSH++ATEKDLKDIEKEMRKEVDEAIAQAK SPMPD 
Sbjct: 301 RTRDEITGIRQERDPIDRIRKLLLSHEIATEKDLKDIEKEMRKEVDEAIAQAKESPMPDP 360

Query: 361 SELFSNVYVKGFEAEVFGADRKEVRGVLP 385
           +ELFSNVYV GF AE FGADRKEV  VLP
Sbjct: 361 TELFSNVYVNGFGAEAFGADRKEVMAVLP 389

BLAST of CsGy5G022580 vs. ExPASy TrEMBL
Match: A0A0A0KQD6 (Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis sativus OX=3659 GN=Csa_5G603950 PE=4 SV=1)

HSP 1 Score: 762 bits (1967), Expect = 7.80e-278
Identity = 385/385 (100.00%), Postives = 385/385 (100.00%), Query Frame = 0

Query: 1   MSHSRISSSSILKPLSSSFRFLSSASHLTIQTSVPFTAHKCEPPSRSVDTSPNELLAFFR 60
           MSHSRISSSSILKPLSSSFRFLSSASHLTIQTSVPFTAHKCEPPSRSVDTSPNELLAFFR
Sbjct: 1   MSHSRISSSSILKPLSSSFRFLSSASHLTIQTSVPFTAHKCEPPSRSVDTSPNELLAFFR 60

Query: 61  EMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHCIFLG 120
           EMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHCIFLG
Sbjct: 61  EMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHCIFLG 120

Query: 121 RGGTLLQSFAELMGRQAGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFAQKYS 180
           RGGTLLQSFAELMGRQAGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFAQKYS
Sbjct: 121 RGGTLLQSFAELMGRQAGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFAQKYS 180

Query: 181 KEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSAAYYK 240
           KEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSAAYYK
Sbjct: 181 KEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSAAYYK 240

Query: 241 RGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTYRTRD 300
           RGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTYRTRD
Sbjct: 241 RGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTYRTRD 300

Query: 301 EISGVRQERDPIERIRKLLLSHDLATEKDLKDIEKEMRKEVDEAIAQAKASPMPDLSELF 360
           EISGVRQERDPIERIRKLLLSHDLATEKDLKDIEKEMRKEVDEAIAQAKASPMPDLSELF
Sbjct: 301 EISGVRQERDPIERIRKLLLSHDLATEKDLKDIEKEMRKEVDEAIAQAKASPMPDLSELF 360

Query: 361 SNVYVKGFEAEVFGADRKEVRGVLP 385
           SNVYVKGFEAEVFGADRKEVRGVLP
Sbjct: 361 SNVYVKGFEAEVFGADRKEVRGVLP 385

BLAST of CsGy5G022580 vs. ExPASy TrEMBL
Match: A0A5D3CWE3 (Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G00620 PE=4 SV=1)

HSP 1 Score: 737 bits (1902), Expect = 7.33e-268
Identity = 372/389 (95.63%), Postives = 380/389 (97.69%), Query Frame = 0

Query: 1   MSHSRISSSSILKPLSSSFRFLSSASH----LTIQTSVPFTAHKCEPPSRSVDTSPNELL 60
           MSHS ISSSSILKPLSSSFRFLSSA+H    LTIQTSVPFTAHKCEPPSRSVDTSPNELL
Sbjct: 1   MSHSPISSSSILKPLSSSFRFLSSAAHDSAHLTIQTSVPFTAHKCEPPSRSVDTSPNELL 60

Query: 61  AFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC 120
           AFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC
Sbjct: 61  AFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC 120

Query: 121 IFLGRGGTLLQSFAELMGRQAGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA 180
            FLGRGGTLL++FAELMGRQ GCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA
Sbjct: 121 TFLGRGGTLLEAFAELMGRQVGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA 180

Query: 181 QKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSA 240
           QKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKS+
Sbjct: 181 QKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSS 240

Query: 241 AYYKRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY 300
           AYYKRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY
Sbjct: 241 AYYKRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY 300

Query: 301 RTRDEISGVRQERDPIERIRKLLLSHDLATEKDLKDIEKEMRKEVDEAIAQAKASPMPDL 360
           RTRDEISGVRQERDPIERIRKLLLSH++ATEKDLKDIEKEMRK+VDEAIAQAKASPMPD 
Sbjct: 301 RTRDEISGVRQERDPIERIRKLLLSHEIATEKDLKDIEKEMRKKVDEAIAQAKASPMPDA 360

Query: 361 SELFSNVYVKGFEAEVFGADRKEVRGVLP 385
           SELF+NVYVKGFE EVFGADRKEVRGVLP
Sbjct: 361 SELFTNVYVKGFETEVFGADRKEVRGVLP 389

BLAST of CsGy5G022580 vs. ExPASy TrEMBL
Match: A0A1S3BE63 (Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis melo OX=3656 GN=LOC103489051 PE=4 SV=1)

HSP 1 Score: 737 bits (1902), Expect = 7.33e-268
Identity = 372/389 (95.63%), Postives = 380/389 (97.69%), Query Frame = 0

Query: 1   MSHSRISSSSILKPLSSSFRFLSSASH----LTIQTSVPFTAHKCEPPSRSVDTSPNELL 60
           MSHS ISSSSILKPLSSSFRFLSSA+H    LTIQTSVPFTAHKCEPPSRSVDTSPNELL
Sbjct: 1   MSHSPISSSSILKPLSSSFRFLSSAAHDSAHLTIQTSVPFTAHKCEPPSRSVDTSPNELL 60

Query: 61  AFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC 120
           AFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC
Sbjct: 61  AFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC 120

Query: 121 IFLGRGGTLLQSFAELMGRQAGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA 180
            FLGRGGTLL++FAELMGRQ GCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA
Sbjct: 121 TFLGRGGTLLEAFAELMGRQVGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA 180

Query: 181 QKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSA 240
           QKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKS+
Sbjct: 181 QKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSS 240

Query: 241 AYYKRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY 300
           AYYKRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY
Sbjct: 241 AYYKRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY 300

Query: 301 RTRDEISGVRQERDPIERIRKLLLSHDLATEKDLKDIEKEMRKEVDEAIAQAKASPMPDL 360
           RTRDEISGVRQERDPIERIRKLLLSH++ATEKDLKDIEKEMRK+VDEAIAQAKASPMPD 
Sbjct: 301 RTRDEISGVRQERDPIERIRKLLLSHEIATEKDLKDIEKEMRKKVDEAIAQAKASPMPDA 360

Query: 361 SELFSNVYVKGFEAEVFGADRKEVRGVLP 385
           SELF+NVYVKGFE EVFGADRKEVRGVLP
Sbjct: 361 SELFTNVYVKGFETEVFGADRKEVRGVLP 389

BLAST of CsGy5G022580 vs. ExPASy TrEMBL
Match: A0A6J1FTT3 (Pyruvate dehydrogenase E1 component subunit alpha OS=Cucurbita moschata OX=3662 GN=LOC111448051 PE=4 SV=1)

HSP 1 Score: 687 bits (1772), Expect = 4.73e-248
Identity = 348/389 (89.46%), Postives = 361/389 (92.80%), Query Frame = 0

Query: 1   MSHSRISSSSILKPLSSSFRFLSSA----SHLTIQTSVPFTAHKCEPPSRSVDTSPNELL 60
           MS  R  SSSILKP SSS+   SSA    S LTI+TSVPFTAHKCE PSRSV TSPNELL
Sbjct: 1   MSLYRTPSSSILKPFSSSYPLFSSAAGHYSDLTIETSVPFTAHKCETPSRSVHTSPNELL 60

Query: 61  AFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC 120
           AFFR+MALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC
Sbjct: 61  AFFRQMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC 120

Query: 121 IFLGRGGTLLQSFAELMGRQAGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA 180
            FL RGGTLL++ AELMGRQAGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA
Sbjct: 121 TFLRRGGTLLEALAELMGRQAGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA 180

Query: 181 QKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSA 240
           QKYSK+GTVTF LYGDGAANQGQLFEALNISALWDLPVI VCENNHYGMGTAEWRAAKS 
Sbjct: 181 QKYSKDGTVTFVLYGDGAANQGQLFEALNISALWDLPVIFVCENNHYGMGTAEWRAAKSP 240

Query: 241 AYYKRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY 300
           AYYKRGDYVPG+KVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY
Sbjct: 241 AYYKRGDYVPGIKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY 300

Query: 301 RTRDEISGVRQERDPIERIRKLLLSHDLATEKDLKDIEKEMRKEVDEAIAQAKASPMPDL 360
           RTRDEI+G+RQERDPI+RIRKLLLSH++ATEKDLKDIEKEMRKEVDEAIAQAK SPMPD 
Sbjct: 301 RTRDEITGIRQERDPIDRIRKLLLSHEIATEKDLKDIEKEMRKEVDEAIAQAKESPMPDP 360

Query: 361 SELFSNVYVKGFEAEVFGADRKEVRGVLP 385
           +ELFSNVYV GF AE FGADRKEV  VLP
Sbjct: 361 TELFSNVYVNGFGAEAFGADRKEVMAVLP 389

BLAST of CsGy5G022580 vs. ExPASy TrEMBL
Match: A0A6J1FWM9 (Pyruvate dehydrogenase E1 component subunit alpha OS=Cucurbita moschata OX=3662 GN=LOC111448051 PE=4 SV=1)

HSP 1 Score: 674 bits (1739), Expect = 8.16e-243
Identity = 344/388 (88.66%), Postives = 358/388 (92.27%), Query Frame = 0

Query: 1   MSHSRISSSSILKPLSSSFRFLSSA----SHLTIQTSVPFTAHKCEPPSRSVDTSPNELL 60
           MS  R  SSSILKP SSS+   SSA    S LTI+TSVPFTAHKCE PSRSV TSPNELL
Sbjct: 1   MSLYRTPSSSILKPFSSSYPLFSSAAGHYSDLTIETSVPFTAHKCETPSRSVHTSPNELL 60

Query: 61  AFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC 120
           AFFR+MALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC
Sbjct: 61  AFFRQMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC 120

Query: 121 IFLGRGGTLLQSFAELMGRQAGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA 180
            FL RGGTLL++ AELMGRQAGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA
Sbjct: 121 TFLRRGGTLLEALAELMGRQAGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA 180

Query: 181 QKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSA 240
           QKYSK+GTVTF LYGDGAANQGQLFEALNISALWDLPVI VCENNHYGMGTAEWRAAKS 
Sbjct: 181 QKYSKDGTVTFVLYGDGAANQGQLFEALNISALWDLPVIFVCENNHYGMGTAEWRAAKSP 240

Query: 241 AYYKRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY 300
           AYYKRGDYVPG+KVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY
Sbjct: 241 AYYKRGDYVPGIKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY 300

Query: 301 RTRDEISGVRQERDPIERIRKLLLSHDLATEKDLKDIEKEMRKEVDEAIAQAKASPMPDL 360
           RTRDEI+G+RQERDPI+RIRKLLLSH++ATEKDLKDIEKEMRKEVDEAIAQAK SPMPD 
Sbjct: 301 RTRDEITGIRQERDPIDRIRKLLLSHEIATEKDLKDIEKEMRKEVDEAIAQAKESPMPDP 360

Query: 361 SELFSNVYVKGFEAEVFGADRKEVRGVL 384
           +ELFSNVYV GF AE FGAD  + RG L
Sbjct: 361 TELFSNVYVNGFGAEAFGAD--QTRGGL 386

BLAST of CsGy5G022580 vs. TAIR 10
Match: AT1G59900.1 (pyruvate dehydrogenase complex E1 alpha subunit )

HSP 1 Score: 643.7 bits (1659), Expect = 9.7e-185
Identity = 320/389 (82.26%), Postives = 353/389 (90.75%), Query Frame = 0

Query: 1   MSHSRISSSS--ILKPLSSSFRFLSS--ASHLTIQTSVPFTAHKCEPPSRSVDTSPNELL 60
           M+ SR+SS S  I +P S++F  L S   + +TI+TS+PFTAH C+PPSRSV++S  ELL
Sbjct: 1   MALSRLSSRSNIITRPFSAAFSRLISTDTTPITIETSLPFTAHLCDPPSRSVESSSQELL 60

Query: 61  AFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAYRDHC 120
            FFR MALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVA+GMEAAITKKDAIITAYRDHC
Sbjct: 61  DFFRTMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAIGMEAAITKKDAIITAYRDHC 120

Query: 121 IFLGRGGTLLQSFAELMGRQAGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCGLAFA 180
           IFLGRGG+L + F+ELMGRQAGCSKGKGGSMHFYKK++ FYGGHGIVGAQVPLGCG+AFA
Sbjct: 121 IFLGRGGSLHEVFSELMGRQAGCSKGKGGSMHFYKKESSFYGGHGIVGAQVPLGCGIAFA 180

Query: 181 QKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRAAKSA 240
           QKY+KE  VTFALYGDGAANQGQLFEALNISALWDLP ILVCENNHYGMGTAEWRAAKS 
Sbjct: 181 QKYNKEEAVTFALYGDGAANQGQLFEALNISALWDLPAILVCENNHYGMGTAEWRAAKSP 240

Query: 241 AYYKRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDPGSTY 300
           +YYKRGDYVPGLKVDGMDA AVKQACKFAK+HAL+ GPIILEMDTYRYHGHSMSDPGSTY
Sbjct: 241 SYYKRGDYVPGLKVDGMDAFAVKQACKFAKQHALEKGPIILEMDTYRYHGHSMSDPGSTY 300

Query: 301 RTRDEISGVRQERDPIERIRKLLLSHDLATEKDLKDIEKEMRKEVDEAIAQAKASPMPDL 360
           RTRDEISGVRQERDPIERI+KL+LSHDLATEK+LKD+EKE+RKEVD+AIA+AK  PMP+ 
Sbjct: 301 RTRDEISGVRQERDPIERIKKLVLSHDLATEKELKDMEKEIRKEVDDAIAKAKDCPMPEP 360

Query: 361 SELFSNVYVKGFEAEVFGADRKEVRGVLP 386
           SELF+NVYVKGF  E FG DRKEV+  LP
Sbjct: 361 SELFTNVYVKGFGTESFGPDRKEVKASLP 389

BLAST of CsGy5G022580 vs. TAIR 10
Match: AT1G24180.1 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 630.6 bits (1625), Expect = 8.5e-181
Identity = 314/393 (79.90%), Postives = 350/393 (89.06%), Query Frame = 0

Query: 1   MSHSRISSSS--ILKP----LSSSFR--FLSSASHLTIQTSVPFTAHKCEPPSRSVDTSP 60
           M+ SR+SS S   LKP    L SS R    + +S +TI+T+VPFT+H CE PSRSV+TS 
Sbjct: 1   MALSRLSSRSNTFLKPAITALPSSIRRHVSTDSSPITIETAVPFTSHLCESPSRSVETSS 60

Query: 61  NELLAFFREMALMRRMEIAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAY 120
            E+LAFFR+MA MRRMEIAADSLYKAKLIRGFCHLYDGQEA+AVGMEAAITKKDAIIT+Y
Sbjct: 61  EEILAFFRDMARMRRMEIAADSLYKAKLIRGFCHLYDGQEALAVGMEAAITKKDAIITSY 120

Query: 121 RDHCIFLGRGGTLLQSFAELMGRQAGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCG 180
           RDHC F+GRGG L+ +F+ELMGR+ GCS GKGGSMHFYKKDA FYGGHGIVGAQ+PLGCG
Sbjct: 121 RDHCTFIGRGGKLVDAFSELMGRKTGCSHGKGGSMHFYKKDASFYGGHGIVGAQIPLGCG 180

Query: 181 LAFAQKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMGTAEWRA 240
           LAFAQKY+K+  VTFALYGDGAANQGQLFEALNISALWDLP ILVCENNHYGMGTA WR+
Sbjct: 181 LAFAQKYNKDEAVTFALYGDGAANQGQLFEALNISALWDLPAILVCENNHYGMGTATWRS 240

Query: 241 AKSAAYYKRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDP 300
           AKS AY+KRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDP
Sbjct: 241 AKSPAYFKRGDYVPGLKVDGMDALAVKQACKFAKEHALKNGPIILEMDTYRYHGHSMSDP 300

Query: 301 GSTYRTRDEISGVRQERDPIERIRKLLLSHDLATEKDLKDIEKEMRKEVDEAIAQAKASP 360
           GSTYRTRDEISGVRQ RDPIER+RKLLL+HD+ATEK+LKD+EKE+RKEVD+A+AQAK SP
Sbjct: 301 GSTYRTRDEISGVRQVRDPIERVRKLLLTHDIATEKELKDMEKEIRKEVDDAVAQAKESP 360

Query: 361 MPDLSELFSNVYVKGFEAEVFGADRKEVRGVLP 386
           +PD SELF+N+YVK    E FGADRKE++  LP
Sbjct: 361 IPDASELFTNMYVKDCGVESFGADRKELKVTLP 393

BLAST of CsGy5G022580 vs. TAIR 10
Match: AT1G01090.1 (pyruvate dehydrogenase E1 alpha )

HSP 1 Score: 236.5 bits (602), Expect = 3.6e-62
Identity = 132/328 (40.24%), Postives = 191/328 (58.23%), Query Frame = 0

Query: 54  ELLAFFREMALMRRME-IAADSLYKAKLIRGFCHLYDGQEAVAVGMEAAITKKDAIITAY 113
           E L  + +M L R  E + A   Y+ K+  GF HLY+GQEAV+ G    +TK D++++ Y
Sbjct: 83  EGLELYEDMILGRSFEDMCAQMYYRGKMF-GFVHLYNGQEAVSTGFIKLLTKSDSVVSTY 142

Query: 114 RDHCIFLGRGGTLLQSFAELMGRQAGCSKGKGGSMHFYKKDAGFYGGHGIVGAQVPLGCG 173
           RDH   L +G +     +EL G+  GC +G+GGSMH + K+    GG   +G  +P+  G
Sbjct: 143 RDHVHALSKGVSARAVMSELFGKVTGCCRGQGGSMHMFSKEHNMLGGFAFIGEGIPVATG 202

Query: 174 LAFAQKYSKE------GTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENNHYGMG 233
            AF+ KY +E        VT A +GDG  N GQ FE LN++AL+ LP+I V ENN + +G
Sbjct: 203 AAFSSKYRREVLKQDCDDVTVAFFGDGTCNNGQFFECLNMAALYKLPIIFVVENNLWAIG 262

Query: 234 TAEWRAAKSAAYYKRGDY--VPGLKVDGMDALAVKQACKFAKEHALK-NGPIILEMDTYR 293
            +  RA      +K+G    +PG+ VDGMD L V++  K A   A +  GP ++E +TYR
Sbjct: 263 MSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAVTRARRGEGPTLVECETYR 322

Query: 294 YHGHSMSDPGSTYRTRDEISGVR-QERDPIERIRKLLLSHDLATEKDLKDIEKEMRKEVD 353
           + GHS++DP      RD     +   RDPI  ++K L+ + LA E +LK IEK++ + V+
Sbjct: 323 FRGHSLADPD---ELRDAAEKAKYAARDPIAALKKYLIENKLAKEAELKSIEKKIDELVE 382

Query: 354 EAIAQAKASPMPDLSELFSNVYV--KGF 369
           EA+  A ASP P  S+L  NV+   KGF
Sbjct: 383 EAVEFADASPQPGRSQLLENVFADPKGF 406

BLAST of CsGy5G022580 vs. TAIR 10
Match: AT5G09300.1 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 151.4 bits (381), Expect = 1.5e-36
Identity = 93/326 (28.53%), Postives = 164/326 (50.31%), Query Frame = 0

Query: 45  SRSVDTSPNELLAFFREMALMRRMEIAADSLYKAKLIRGFCHLYD---GQEAVAVGMEAA 104
           S+ V  S    +  + +M  ++ M    D+++     +G    Y    G+EA+ +   AA
Sbjct: 120 SQFVQVSEEVAVKIYSDMVTLQIM----DNIFYEAQRQGRLSFYATAIGEEAINIASAAA 179

Query: 105 ITKKDAIITAYRDHCIFLGRGGTLLQSFAELMGRQAGCSKGKGGSMHFYKKDAGFYGGHG 164
           +T +D I   YR+  + L RG TL +   +  G ++   KG+   +H+      ++    
Sbjct: 180 LTPQDVIFPQYREPGVLLWRGFTLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYFTVSA 239

Query: 165 IVGAQVPLGCGLAFAQKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENN 224
            +  Q+P   G A++ K  K+       +GDG  ++G    ALNI+A+ + PV+ +C NN
Sbjct: 240 TIATQLPNAVGAAYSLKMDKKDACAVTYFGDGGTSEGDFHAALNIAAVMEAPVLFICRNN 299

Query: 225 HYGMGTAEWRAAKSAAYYKRGDY--VPGLKVDGMDALAVKQACKFAKEHALK-NGPIILE 284
            + + T      +S     +G    +  ++VDG DALA+  A   A+E A++   PI++E
Sbjct: 300 GWAISTPTSDQFRSDGVVVKGRAYGIRSIRVDGNDALAMYSAVHTAREMAIREQRPILIE 359

Query: 285 MDTYRYHGHSMSDPGSTYRTRDEISGVRQERDPIERIRKLLLSHDLATEKDLKDIEKEMR 344
             TYR   HS SD  + YR+  EI    + R+P+ R R  + S+   ++K   D+   ++
Sbjct: 360 ALTYRVGHHSTSDDSTRYRSAGEIEWWNKARNPLSRFRTWIESNGWWSDKTESDLRSRIK 419

Query: 345 KEVDEAIAQAKASPMPDLSELFSNVY 365
           KE+ EA+  A+ +  P+L  +FS+VY
Sbjct: 420 KEMLEALRVAEKTEKPNLQNMFSDVY 441

BLAST of CsGy5G022580 vs. TAIR 10
Match: AT5G09300.2 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 151.4 bits (381), Expect = 1.5e-36
Identity = 93/326 (28.53%), Postives = 164/326 (50.31%), Query Frame = 0

Query: 45  SRSVDTSPNELLAFFREMALMRRMEIAADSLYKAKLIRGFCHLYD---GQEAVAVGMEAA 104
           S+ V  S    +  + +M  ++ M    D+++     +G    Y    G+EA+ +   AA
Sbjct: 49  SQFVQVSEEVAVKIYSDMVTLQIM----DNIFYEAQRQGRLSFYATAIGEEAINIASAAA 108

Query: 105 ITKKDAIITAYRDHCIFLGRGGTLLQSFAELMGRQAGCSKGKGGSMHFYKKDAGFYGGHG 164
           +T +D I   YR+  + L RG TL +   +  G ++   KG+   +H+      ++    
Sbjct: 109 LTPQDVIFPQYREPGVLLWRGFTLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYFTVSA 168

Query: 165 IVGAQVPLGCGLAFAQKYSKEGTVTFALYGDGAANQGQLFEALNISALWDLPVILVCENN 224
            +  Q+P   G A++ K  K+       +GDG  ++G    ALNI+A+ + PV+ +C NN
Sbjct: 169 TIATQLPNAVGAAYSLKMDKKDACAVTYFGDGGTSEGDFHAALNIAAVMEAPVLFICRNN 228

Query: 225 HYGMGTAEWRAAKSAAYYKRGDY--VPGLKVDGMDALAVKQACKFAKEHALK-NGPIILE 284
            + + T      +S     +G    +  ++VDG DALA+  A   A+E A++   PI++E
Sbjct: 229 GWAISTPTSDQFRSDGVVVKGRAYGIRSIRVDGNDALAMYSAVHTAREMAIREQRPILIE 288

Query: 285 MDTYRYHGHSMSDPGSTYRTRDEISGVRQERDPIERIRKLLLSHDLATEKDLKDIEKEMR 344
             TYR   HS SD  + YR+  EI    + R+P+ R R  + S+   ++K   D+   ++
Sbjct: 289 ALTYRVGHHSTSDDSTRYRSAGEIEWWNKARNPLSRFRTWIESNGWWSDKTESDLRSRIK 348

Query: 345 KEVDEAIAQAKASPMPDLSELFSNVY 365
           KE+ EA+  A+ +  P+L  +FS+VY
Sbjct: 349 KEMLEALRVAEKTEKPNLQNMFSDVY 370

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P529025.6e-18582.12Pyruvate dehydrogenase E1 component subunit alpha, mitochondrial OS=Pisum sativu... [more]
P529011.4e-18382.26Pyruvate dehydrogenase E1 component subunit alpha-1, mitochondrial OS=Arabidopsi... [more]
Q6Z5N41.9e-18084.25Pyruvate dehydrogenase E1 component subunit alpha-1, mitochondrial OS=Oryza sati... [more]
Q8H1Y01.2e-17979.90Pyruvate dehydrogenase E1 component subunit alpha-2, mitochondrial OS=Arabidopsi... [more]
P529031.2e-17981.05Pyruvate dehydrogenase E1 component subunit alpha, mitochondrial OS=Solanum tube... [more]
Match NameE-valueIdentityDescription
XP_004135218.11.61e-277100.00pyruvate dehydrogenase E1 component subunit alpha, mitochondrial [Cucumis sativu... [more]
XP_008446275.11.51e-26795.63PREDICTED: pyruvate dehydrogenase E1 component subunit alpha, mitochondrial [Cuc... [more]
XP_038891336.13.65e-25993.32pyruvate dehydrogenase E1 component subunit alpha, mitochondrial [Benincasa hisp... [more]
XP_022943269.19.76e-24889.46pyruvate dehydrogenase E1 component subunit alpha, mitochondrial-like isoform X2... [more]
XP_023548204.19.76e-24889.46pyruvate dehydrogenase E1 component subunit alpha, mitochondrial-like [Cucurbita... [more]
Match NameE-valueIdentityDescription
A0A0A0KQD67.80e-278100.00Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis sativus OX=3659 GN=... [more]
A0A5D3CWE37.33e-26895.63Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis melo var. makuwa OX... [more]
A0A1S3BE637.33e-26895.63Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis melo OX=3656 GN=LOC... [more]
A0A6J1FTT34.73e-24889.46Pyruvate dehydrogenase E1 component subunit alpha OS=Cucurbita moschata OX=3662 ... [more]
A0A6J1FWM98.16e-24388.66Pyruvate dehydrogenase E1 component subunit alpha OS=Cucurbita moschata OX=3662 ... [more]
Match NameE-valueIdentityDescription
AT1G59900.19.7e-18582.26pyruvate dehydrogenase complex E1 alpha subunit [more]
AT1G24180.18.5e-18179.90Thiamin diphosphate-binding fold (THDP-binding) superfamily protein [more]
AT1G01090.13.6e-6240.24pyruvate dehydrogenase E1 alpha [more]
AT5G09300.11.5e-3628.53Thiamin diphosphate-binding fold (THDP-binding) superfamily protein [more]
AT5G09300.21.5e-3628.53Thiamin diphosphate-binding fold (THDP-binding) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 327..347
NoneNo IPR availableGENE3D3.40.50.970coord: 1..380
e-value: 5.7E-133
score: 445.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 288..307
NoneNo IPR availablePANTHERPTHR11516PYRUVATE DEHYDROGENASE E1 COMPONENT, ALPHA SUBUNIT BACTERIAL AND ORGANELLARcoord: 9..385
NoneNo IPR availablePANTHERPTHR11516:SF65PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT ALPHA, MITOCHONDRIALcoord: 9..385
NoneNo IPR availableCDDcd02000TPP_E1_PDC_ADC_BCADCcoord: 58..348
e-value: 9.91524E-143
score: 405.341
IPR017597Pyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit, subgroup yTIGRFAMTIGR03182TIGR03182coord: 53..365
e-value: 1.2E-126
score: 419.8
IPR001017Dehydrogenase, E1 componentPFAMPF00676E1_dhcoord: 61..355
e-value: 4.1E-118
score: 393.7
IPR029061Thiamin diphosphate-binding foldSUPERFAMILY52518Thiamin diphosphate-binding fold (THDP-binding)coord: 44..366

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy5G022580.2CsGy5G022580.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006086 acetyl-CoA biosynthetic process from pyruvate
biological_process GO:0006096 glycolytic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0043231 intracellular membrane-bounded organelle
molecular_function GO:0004739 pyruvate dehydrogenase (acetyl-transferring) activity
molecular_function GO:0016624 oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor