CsGy5G019830 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy5G019830
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionGRAM domain-containing protein
LocationGy14Chr5: 26069875 .. 26071157 (-)
RNA-Seq ExpressionCsGy5G019830
SyntenyCsGy5G019830
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CATCCCACCAAATCCAAAGCTCCATCATACTTAAATCTCTAAAACATCTCAAAATTATTTCTATCTTTTCTTCCATTTGTAAAGAAAAATGAAAACTACGATTCAAGAACATATGCTAGAATTTCAGATGACATTGGCTCTAGCAAAAGCTAGATATTTGGGCCATTCCTCGAAGCATCTTGACATATCTTCTTCAAACAATGGATCTCTTAAGAAAGGTAAACGAGATTAAGATTGAAGACTAAGAGTTTTTTTAAATAGTTTTTGCTAACTAATTTGGTTTTTAAATTGTTGTACAGGTAATGGAAAAGATTGGATCTTGAATAGACTCAACAAAAATGAAGGAAAAACAGACATGTTAGACGATATCATTCATGCACTCCAAGAACATGGTAAGTCTTCTTTAAAAATTCGAGTCTTTTTCGTCCAAAATAATTTGAATTGGAATTTGACATGTGCCCAATTACTTTGAATGAAAACTATCCTATGTTAGTAGTTGTGATGATTTGGAACATTATAGAGCGTAATTGTGATGATTTTGAGGTTTGAATTTTGCAGTGAAATTAGGAGGTAAGATATCAGAAAAAATAAAGGGAAAGTTGGGTTTAGGAGGAAGAACTTTAAGAGTTGGTGGGATGAGAAAAATCTACAAGAAATTATTTCCAATGAATGAAGAAGAGAAGCTTTTGAAGGTTTCACAATGTTACTTATCAACAACGGCTGGACCTTTGGGTGGCCTTCTCTTCCTTTCCACTCACAAAATTGCTTTTTGCAGCGCCAAATCCATCACAGTACTCTCTCCCCCAAATGGAGATAATGATTATGTTAGAATTCACTATAAAGTAAGCATCTTTAATTATTATAAGTCTATTATGATATCTATATATCTATATATGTTATATATGAATTAAGATATATATATATTATTAATGATGAAGGTTAATTAATTTTTACAGGTTGTGATTCCAATGGAGAAGGTGATGGGAGTAAATGAAAGTGATCAGAAGAATGTTAAGACAGATTCAGAAAAATATATACAAATAGTGACAGTGGACAACCTTGAGTTTTGGTTTATGGGATTATTAAATAATTATCAATCAATTTTCAATTCTCTTCAAGTGTTAGTAAAAACAAAACTTTGATTGTTTTTTGTTTATATATATATACTCATTGGCTTCTTGCTGATGAAGAGATATGTTTGTATATCTTGAAGAATAGAACAGTGGTTAGTTTGTACATATCATTTATTAAATTCAATCTCTCTAATGAGAAAATTAACTCCA

mRNA sequence

CATCCCACCAAATCCAAAGCTCCATCATACTTAAATCTCTAAAACATCTCAAAATTATTTCTATCTTTTCTTCCATTTGTAAAGAAAAATGAAAACTACGATTCAAGAACATATGCTAGAATTTCAGATGACATTGGCTCTAGCAAAAGCTAGATATTTGGGCCATTCCTCGAAGCATCTTGACATATCTTCTTCAAACAATGGATCTCTTAAGAAAGGTAATGGAAAAGATTGGATCTTGAATAGACTCAACAAAAATGAAGGAAAAACAGACATGTTAGACGATATCATTCATGCACTCCAAGAACATGTGAAATTAGGAGGTAAGATATCAGAAAAAATAAAGGGAAAGTTGGGTTTAGGAGGAAGAACTTTAAGAGTTGGTGGGATGAGAAAAATCTACAAGAAATTATTTCCAATGAATGAAGAAGAGAAGCTTTTGAAGGTTTCACAATGTTACTTATCAACAACGGCTGGACCTTTGGGTGGCCTTCTCTTCCTTTCCACTCACAAAATTGCTTTTTGCAGCGCCAAATCCATCACAGTACTCTCTCCCCCAAATGGAGATAATGATTATGTTAGAATTCACTATAAAGTTGTGATTCCAATGGAGAAGGTGATGGGAGTAAATGAAAGTGATCAGAAGAATGTTAAGACAGATTCAGAAAAATATATACAAATAGTGACAGTGGACAACCTTGAGTTTTGGTTTATGGGATTATTAAATAATTATCAATCAATTTTCAATTCTCTTCAAGTGTTAGTAAAAACAAAACTTTGATTGTTTTTTGTTTATATATATATACTCATTGGCTTCTTGCTGATGAAGAGATATGTTTGTATATCTTGAAGAATAGAACAGTGGTTAGTTTGTACATATCATTTATTAAATTCAATCTCTCTAATGAGAAAATTAACTCCA

Coding sequence (CDS)

ATGAAAACTACGATTCAAGAACATATGCTAGAATTTCAGATGACATTGGCTCTAGCAAAAGCTAGATATTTGGGCCATTCCTCGAAGCATCTTGACATATCTTCTTCAAACAATGGATCTCTTAAGAAAGGTAATGGAAAAGATTGGATCTTGAATAGACTCAACAAAAATGAAGGAAAAACAGACATGTTAGACGATATCATTCATGCACTCCAAGAACATGTGAAATTAGGAGGTAAGATATCAGAAAAAATAAAGGGAAAGTTGGGTTTAGGAGGAAGAACTTTAAGAGTTGGTGGGATGAGAAAAATCTACAAGAAATTATTTCCAATGAATGAAGAAGAGAAGCTTTTGAAGGTTTCACAATGTTACTTATCAACAACGGCTGGACCTTTGGGTGGCCTTCTCTTCCTTTCCACTCACAAAATTGCTTTTTGCAGCGCCAAATCCATCACAGTACTCTCTCCCCCAAATGGAGATAATGATTATGTTAGAATTCACTATAAAGTTGTGATTCCAATGGAGAAGGTGATGGGAGTAAATGAAAGTGATCAGAAGAATGTTAAGACAGATTCAGAAAAATATATACAAATAGTGACAGTGGACAACCTTGAGTTTTGGTTTATGGGATTATTAAATAATTATCAATCAATTTTCAATTCTCTTCAAGTGTTAGTAAAAACAAAACTTTGA

Protein sequence

MKTTIQEHMLEFQMTLALAKARYLGHSSKHLDISSSNNGSLKKGNGKDWILNRLNKNEGKTDMLDDIIHALQEHVKLGGKISEKIKGKLGLGGRTLRVGGMRKIYKKLFPMNEEEKLLKVSQCYLSTTAGPLGGLLFLSTHKIAFCSAKSITVLSPPNGDNDYVRIHYKVVIPMEKVMGVNESDQKNVKTDSEKYIQIVTVDNLEFWFMGLLNNYQSIFNSLQVLVKTKL*
Homology
BLAST of CsGy5G019830 vs. ExPASy Swiss-Prot
Match: Q9FMW4 (Putative GEM-like protein 8 OS=Arabidopsis thaliana OX=3702 GN=At5g23370 PE=3 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 7.7e-41
Identity = 93/197 (47.21%), Postives = 127/197 (64.47%), Query Frame = 0

Query: 27  SSKHLDISSSNNGSLKKGNGKDWILNRLNKNEGKTDMLDDIIHALQEHVKLGGKISEKIK 86
           S   L I +S+  S   G GK  +  + N         D   + +++  KLG K++E +K
Sbjct: 29  SINKLQIPTSSKFSFLTGKGKSMLRKKKN---------DSFTNGVRDQDKLGPKLTETVK 88

Query: 87  GKLGLGGRTLRVGGMRKIYKKLFPMNEEEKLLKVSQCYLSTTAGPLGGLLFLSTHKIAFC 146
            KL LG R L++GG+ KIYK+LF +++EEKL K  QCYLSTTAGP+ GLLF+S+ KIAFC
Sbjct: 89  RKLSLGARILQMGGLEKIYKRLFKVSDEEKLFKAYQCYLSTTAGPIAGLLFISSKKIAFC 148

Query: 147 SAKSITVLSPPNGDNDYVRIHYKVVIPMEKVMGVNESDQKNVKTDSEKYIQIVTVDNLEF 206
           S +SI V SP    N   R+HYKV IP+ K+ GVN+S  +N    S+KY+++VTVD  +F
Sbjct: 149 SERSIKVASPQGELN---RVHYKVSIPLCKINGVNQS--QNTTKPSQKYLEVVTVDGFDF 208

Query: 207 WFMGLLNNYQSIFNSLQ 224
           WFMG L +YQ  FN L+
Sbjct: 209 WFMGFL-SYQKAFNCLE 210

BLAST of CsGy5G019830 vs. ExPASy Swiss-Prot
Match: Q9FMW6 (GEM-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=At5g23350 PE=2 SV=2)

HSP 1 Score: 161.4 bits (407), Expect = 1.2e-38
Identity = 87/192 (45.31%), Postives = 126/192 (65.62%), Query Frame = 0

Query: 32  DISSSNNGSLKKGNGKDWILNRLNKNEGKTDMLDDIIHALQEHVKLGGKISEKIKGKLGL 91
           D +S NN  +   +   ++  +  K+  +    D   +  ++  KLG K++E +K KL L
Sbjct: 26  DPASINNHQIPTSSKVSYLTGK-GKSMLRKKKTDSFTNGARDQDKLGPKLTETVKRKLSL 85

Query: 92  GGRTLRVGGMRKIYKKLFPMNEEEKLLKVSQCYLSTTAGPLGGLLFLSTHKIAFCSAKSI 151
           G + L++GG+ KIYK+LF + ++EKL K  QCYLSTTAGP+ GLLF+S+ KIAFCS +SI
Sbjct: 86  GAKILQMGGLEKIYKRLFKVCDQEKLFKAYQCYLSTTAGPIAGLLFISSKKIAFCSERSI 145

Query: 152 TVLSPPNGDNDYVRIHYKVVIPMEKVMGVNESDQKNVKTDSEKYIQIVTVDNLEFWFMGL 211
            V SP        R+HYKV IP+ K+ GVN+S  +N K  S+KY++IVT+DN +FWFMG 
Sbjct: 146 KVASP---QGVLSRVHYKVSIPLCKINGVNQS--QNTKKPSQKYLEIVTIDNFDFWFMGF 205

Query: 212 LNNYQSIFNSLQ 224
           + +YQ  FN L+
Sbjct: 206 V-SYQKAFNCLE 210

BLAST of CsGy5G019830 vs. ExPASy Swiss-Prot
Match: Q9FTA0 (GEM-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=At5g08350 PE=2 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 1.6e-38
Identity = 82/164 (50.00%), Postives = 115/164 (70.12%), Query Frame = 0

Query: 60  KTDMLDDIIHALQEHVKLGGKISEKIKGKLGLGGRTLRVGGMRKIYKKLFPMNEEEKLLK 119
           K    D   + +++  K+  K++E +K KL LG R L+VGG+ KI+K+LF ++E EKL K
Sbjct: 52  KRKKTDGFTNGVRDQSKIRPKLTETVKRKLSLGARILQVGGLEKIFKRLFRVSEGEKLFK 111

Query: 120 VSQCYLSTTAGPLGGLLFLSTHKIAFCSAKSITVLSPPNGDNDYVRIHYKVVIPMEKVMG 179
           + QCYLSTTAGP+ GLLF+S+ K+AFCS +SI V SP     D +R+HYKV IP+ K+  
Sbjct: 112 MYQCYLSTTAGPIAGLLFISSKKMAFCSERSIKVDSP---QGDIIRVHYKVSIPLCKIDR 171

Query: 180 VNESDQKNVKTDSEKYIQIVTVDNLEFWFMGLLNNYQSIFNSLQ 224
           VN+S  +N K  S+KY+++VTVD  +FWFMG L +YQ  FN L+
Sbjct: 172 VNQS--QNTKKPSQKYLEVVTVDGFDFWFMGFL-SYQKAFNCLE 209

BLAST of CsGy5G019830 vs. ExPASy Swiss-Prot
Match: Q9FMW5 (GEM-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=At5g23360 PE=2 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 2.7e-38
Identity = 80/159 (50.31%), Postives = 112/159 (70.44%), Query Frame = 0

Query: 65  DDIIHALQEHVKLGGKISEKIKGKLGLGGRTLRVGGMRKIYKKLFPMNEEEKLLKVSQCY 124
           D   +  ++  KLG K++E +K KL LG + L++GG+ KIYK+LF + ++EKL K  QCY
Sbjct: 50  DSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDKEKLFKAYQCY 109

Query: 125 LSTTAGPLGGLLFLSTHKIAFCSAKSITVLSPPNGDNDYVRIHYKVVIPMEKVMGVNESD 184
           LSTT G + GLLF+S+ KIAFCS +SI V SP     D  R+HYKV IP+ K+ GVN+S 
Sbjct: 110 LSTTEGSIAGLLFISSKKIAFCSERSIKVTSP---QGDLTRVHYKVSIPLCKINGVNQS- 169

Query: 185 QKNVKTDSEKYIQIVTVDNLEFWFMGLLNNYQSIFNSLQ 224
            +N K  S++Y+++VTVDN +FWFMG + +YQ  FN L+
Sbjct: 170 -QNTKKPSQRYLEVVTVDNYDFWFMGFV-SYQKAFNCLE 202

BLAST of CsGy5G019830 vs. ExPASy Swiss-Prot
Match: Q9SE96 (GEM-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=FIP1 PE=1 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 5.9e-25
Identity = 66/168 (39.29%), Postives = 102/168 (60.71%), Query Frame = 0

Query: 61  TDMLDDIIHALQEHVKLGGKISEKIKGKLGLGGRTLRVGGMRKIYKKLFPMNEEEKLLKV 120
           T   +D+     +H+K G  +++    ++  G + L  GG  K++K+ F    +EKLLK 
Sbjct: 96  TKKAEDLAGNFWQHLKTGPSVADAAVSRIAQGTKILAEGGYEKVFKQTFDCLPDEKLLKT 155

Query: 121 SQCYLSTTAGPLGGLLFLSTHKIAFCSAKSITVLSPPNGDNDYVRIHYKVVIPMEKVMGV 180
             CYLST+AGP+ G+++LSTHK+AF S      LS   G+   +  +YKVV+P  ++  V
Sbjct: 156 YACYLSTSAGPVLGVMYLSTHKLAFSSDNP---LSYKEGEQT-LWSYYKVVLPANQLKAV 215

Query: 181 NESDQKNVKTDSEKYIQIVTVDNLEFWFMGLLNNYQSIFNSLQVLVKT 229
           N S  + V T S+KYIQ++++DN EFWFMG +  Y+S   SLQ  V++
Sbjct: 216 NPSTSR-VNT-SDKYIQVISIDNHEFWFMGFV-TYESAVKSLQEAVQS 256

BLAST of CsGy5G019830 vs. NCBI nr
Match: XP_011655498.1 (putative GEM-like protein 8 [Cucumis sativus] >KGN51564.1 hypothetical protein Csa_008849 [Cucumis sativus])

HSP 1 Score: 455 bits (1170), Expect = 3.00e-161
Identity = 228/230 (99.13%), Postives = 230/230 (100.00%), Query Frame = 0

Query: 1   MKTTIQEHMLEFQMTLALAKARYLGHSSKHLDISSSNNGSLKKGNGKDWILNRLNKNEGK 60
           MKTTIQEHMLEFQMTLALAKARYLGHSSKHLDISSSNNGSLKKGNGKDWILNRLNKNEGK
Sbjct: 1   MKTTIQEHMLEFQMTLALAKARYLGHSSKHLDISSSNNGSLKKGNGKDWILNRLNKNEGK 60

Query: 61  TDMLDDIIHALQEHVKLGGKISEKIKGKLGLGGRTLRVGGMRKIYKKLFPMNEEEKLLKV 120
           TDMLDDIIHALQEHVKLGGKISEKIKGKLGLGGRTLRVGGMRK+YKKLFPMNEEEKLLKV
Sbjct: 61  TDMLDDIIHALQEHVKLGGKISEKIKGKLGLGGRTLRVGGMRKMYKKLFPMNEEEKLLKV 120

Query: 121 SQCYLSTTAGPLGGLLFLSTHKIAFCSAKSITVLSPPNGDNDYVRIHYKVVIPMEKVMGV 180
           SQCYLSTTAGPLGGLLFLSTHKIAFCSAKSITVLSPPNGDNDYVRIHYKVVIPMEKVMGV
Sbjct: 121 SQCYLSTTAGPLGGLLFLSTHKIAFCSAKSITVLSPPNGDNDYVRIHYKVVIPMEKVMGV 180

Query: 181 NESDQKNVKTDSEKYIQIVTVDNLEFWFMGLLNNYQSIFNSLQVLVKTKL 230
           NESDQKNVKTDSEKYIQIVTVDNLEFWFMGLLNNYQSIFNSL+VLVKTKL
Sbjct: 181 NESDQKNVKTDSEKYIQIVTVDNLEFWFMGLLNNYQSIFNSLEVLVKTKL 230

BLAST of CsGy5G019830 vs. NCBI nr
Match: XP_008467050.1 (PREDICTED: putative GEM-like protein 8 [Cucumis melo])

HSP 1 Score: 395 bits (1016), Expect = 8.33e-138
Identity = 208/233 (89.27%), Postives = 217/233 (93.13%), Query Frame = 0

Query: 1   MKTTIQEHMLEFQMTLALAKARYLGHSSKHLDISSSNNGS---LKKGNGKDWILNRLNKN 60
           MKTT+QEH+LEFQM+LALAKARYLGHS KHL ISSSN+GS   +KKGNGKD ILNRLNKN
Sbjct: 1   MKTTLQEHILEFQMSLALAKARYLGHSLKHLRISSSNDGSQSLIKKGNGKDLILNRLNKN 60

Query: 61  EGKTDMLDDIIHALQEHVKLGGKISEKIKGKLGLGGRTLRVGGMRKIYKKLFPMNEEEKL 120
           EGKTDMLDD+IHA+QEH   GGKISEKIKGKLGLG R+LRVGGMRKIYKKLF MNEEEKL
Sbjct: 61  EGKTDMLDDVIHAVQEH---GGKISEKIKGKLGLGARSLRVGGMRKIYKKLFSMNEEEKL 120

Query: 121 LKVSQCYLSTTAGPLGGLLFLSTHKIAFCSAKSITVLSPPNGDNDYVRIHYKVVIPMEKV 180
           LKVSQCYLSTTAGPLGGLLFLSTHKIAFCSAKSIT  SPPNGDNDYVRIHYKVVIPMEKV
Sbjct: 121 LKVSQCYLSTTAGPLGGLLFLSTHKIAFCSAKSIT-FSPPNGDNDYVRIHYKVVIPMEKV 180

Query: 181 MGVNESDQKNVKTDSEKYIQIVTVDNLEFWFMGLLNNYQSIFNSLQVLVKTKL 230
           MGVNES+ KNVKTDSEKYIQIVTVDNLEFWFM  LNNYQSIFNSLQVLVKTKL
Sbjct: 181 MGVNESE-KNVKTDSEKYIQIVTVDNLEFWFMRFLNNYQSIFNSLQVLVKTKL 228

BLAST of CsGy5G019830 vs. NCBI nr
Match: XP_008465496.1 (PREDICTED: GEM-like protein 4 [Cucumis melo] >KAA0034082.1 GEM-like protein 4 [Cucumis melo var. makuwa])

HSP 1 Score: 305 bits (780), Expect = 6.47e-102
Identity = 168/230 (73.04%), Postives = 187/230 (81.30%), Query Frame = 0

Query: 3   TTIQEHMLEFQMTLALAKARYLGH-SSKHLDISSSNN----GSLKKGNGKDWILNRLNKN 62
           TTIQEHML FQM+ AL++ARYLGH  SKHL +SSS++     S+KK NGKD ILNRLNKN
Sbjct: 4   TTIQEHMLGFQMSFALSRARYLGHYPSKHLHVSSSDDRISQSSIKKYNGKDCILNRLNKN 63

Query: 63  EGKTDMLDDIIHALQEHVKLGGKISEKIKGKLGLGGRTLRVGGMRKIYKKLFPMNEEEKL 122
             KTD   +IIHAL+EHVKLG KISE +KGKL LG R LRVGG+RKIYKKLF MNEEEKL
Sbjct: 64  GKKTD---NIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMNEEEKL 123

Query: 123 LKVSQCYLSTTAGPLGGLLFLSTHKIAFCSAKSITVLSPPNGDNDYVRIHYKVVIPMEKV 182
           LKVSQCYLSTTAGPL GLLF+STHKIAFCS KSI + SP NGD  ++RIHYKVVIP EKV
Sbjct: 124 LKVSQCYLSTTAGPLAGLLFISTHKIAFCSDKSIKIASPNNGD--HIRIHYKVVIPKEKV 183

Query: 183 MGVNESDQKNVKTDSEKYIQIVTVDNLEFWFMGLLNNYQSIFNSLQVLVK 227
           M VNES+  NVK  SEKYIQI T+DN EFWFMG LN YQS FNSLQ + +
Sbjct: 184 MRVNESE--NVKKASEKYIQIETLDNFEFWFMGFLN-YQSTFNSLQEVAR 225

BLAST of CsGy5G019830 vs. NCBI nr
Match: XP_011655499.1 (GEM-like protein 4 [Cucumis sativus] >KGN52179.2 hypothetical protein Csa_008572 [Cucumis sativus])

HSP 1 Score: 303 bits (776), Expect = 2.37e-101
Identity = 168/231 (72.73%), Postives = 189/231 (81.82%), Query Frame = 0

Query: 1   MKTTIQEHMLEFQMTLALAKARYLGHSSKHLDISSSNN----GSLKKGNGKDWILNRLNK 60
           MKTTIQEHML FQM+LAL++AR LGHSSKHL +SSS++     S+KK NGKD IL RLNK
Sbjct: 1   MKTTIQEHMLGFQMSLALSRARSLGHSSKHLHVSSSDDRISQSSIKKYNGKDCILKRLNK 60

Query: 61  NEGKTDMLDDIIHALQEHVKLGGKISEKIKGKLGLGGRTLRVGGMRKIYKKLFPMNEEEK 120
           N  KTD   +IIHAL+EHVKLG KISE +KGKL LG R LRVGG+RKIYKKLF M+EEEK
Sbjct: 61  NGKKTD---NIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMSEEEK 120

Query: 121 LLKVSQCYLSTTAGPLGGLLFLSTHKIAFCSAKSITVLSPPNGDNDYVRIHYKVVIPMEK 180
           LLKVSQCYLSTTAGPL GLLF+STHKIAFCS KSI + SP NGD  ++RIHYKVVIP EK
Sbjct: 121 LLKVSQCYLSTTAGPLPGLLFISTHKIAFCSDKSIKIASP-NGD--HIRIHYKVVIPKEK 180

Query: 181 VMGVNESDQKNVKTDSEKYIQIVTVDNLEFWFMGLLNNYQSIFNSLQVLVK 227
           VM VNES+  NVK  SE+YIQI T+DN EFWFMG LN YQS FNSLQ + +
Sbjct: 181 VMRVNESE--NVKKTSERYIQIETLDNFEFWFMGFLN-YQSTFNSLQEVAR 222

BLAST of CsGy5G019830 vs. NCBI nr
Match: XP_008445894.1 (PREDICTED: GEM-like protein 4 [Cucumis melo])

HSP 1 Score: 301 bits (770), Expect = 1.69e-100
Identity = 167/226 (73.89%), Postives = 186/226 (82.30%), Query Frame = 0

Query: 1   MKTTIQEHMLEFQMTLALAKARYLGHSSKHLDISSSNNGS---LKKGNGKDWILNRLNKN 60
           MKTTIQEHML FQM+LAL++ARYLGH  K L +SSS++GS   +KK NGKD ILNRLN N
Sbjct: 1   MKTTIQEHMLGFQMSLALSRARYLGHPLKRLHLSSSDDGSQSSIKKYNGKDCILNRLNTN 60

Query: 61  EGKTDMLDDIIHALQEHVKLGGKISEKIKGKLGLGGRTLRVGGMRKIYKKLFPMNEEEKL 120
             +TD   +IIHAL+EHVKLGGKISE +KGKL LG + LRVGG+RKIYKKLF MNEEEKL
Sbjct: 61  GKRTD---NIIHALREHVKLGGKISEAVKGKLSLGAKILRVGGLRKIYKKLFSMNEEEKL 120

Query: 121 LKVSQCYLSTTAGPLGGLLFLSTHKIAFCSAKSITVLSPPNGDNDYVRIHYKVVIPMEKV 180
           LKVSQCYLSTTAGPL GLLF+STHKIAFCS KSI + SP NGD  ++RI YKVVIP EKV
Sbjct: 121 LKVSQCYLSTTAGPLAGLLFISTHKIAFCSDKSIKIASP-NGD--HIRILYKVVIPKEKV 180

Query: 181 MGVNESDQKNVKTDSEKYIQIVTVDNLEFWFMGLLNNYQSIFNSLQ 223
           M VNES+  NVK  SEKYIQI T+DN EFWFMG LN YQS FNSLQ
Sbjct: 181 MRVNESE--NVKKASEKYIQIETLDNFEFWFMGFLN-YQSTFNSLQ 217

BLAST of CsGy5G019830 vs. ExPASy TrEMBL
Match: A0A0A0KRR9 (GRAM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G579020 PE=3 SV=1)

HSP 1 Score: 455 bits (1170), Expect = 1.45e-161
Identity = 228/230 (99.13%), Postives = 230/230 (100.00%), Query Frame = 0

Query: 1   MKTTIQEHMLEFQMTLALAKARYLGHSSKHLDISSSNNGSLKKGNGKDWILNRLNKNEGK 60
           MKTTIQEHMLEFQMTLALAKARYLGHSSKHLDISSSNNGSLKKGNGKDWILNRLNKNEGK
Sbjct: 1   MKTTIQEHMLEFQMTLALAKARYLGHSSKHLDISSSNNGSLKKGNGKDWILNRLNKNEGK 60

Query: 61  TDMLDDIIHALQEHVKLGGKISEKIKGKLGLGGRTLRVGGMRKIYKKLFPMNEEEKLLKV 120
           TDMLDDIIHALQEHVKLGGKISEKIKGKLGLGGRTLRVGGMRK+YKKLFPMNEEEKLLKV
Sbjct: 61  TDMLDDIIHALQEHVKLGGKISEKIKGKLGLGGRTLRVGGMRKMYKKLFPMNEEEKLLKV 120

Query: 121 SQCYLSTTAGPLGGLLFLSTHKIAFCSAKSITVLSPPNGDNDYVRIHYKVVIPMEKVMGV 180
           SQCYLSTTAGPLGGLLFLSTHKIAFCSAKSITVLSPPNGDNDYVRIHYKVVIPMEKVMGV
Sbjct: 121 SQCYLSTTAGPLGGLLFLSTHKIAFCSAKSITVLSPPNGDNDYVRIHYKVVIPMEKVMGV 180

Query: 181 NESDQKNVKTDSEKYIQIVTVDNLEFWFMGLLNNYQSIFNSLQVLVKTKL 230
           NESDQKNVKTDSEKYIQIVTVDNLEFWFMGLLNNYQSIFNSL+VLVKTKL
Sbjct: 181 NESDQKNVKTDSEKYIQIVTVDNLEFWFMGLLNNYQSIFNSLEVLVKTKL 230

BLAST of CsGy5G019830 vs. ExPASy TrEMBL
Match: A0A1S3CST3 (putative GEM-like protein 8 OS=Cucumis melo OX=3656 GN=LOC103504485 PE=3 SV=1)

HSP 1 Score: 395 bits (1016), Expect = 4.03e-138
Identity = 208/233 (89.27%), Postives = 217/233 (93.13%), Query Frame = 0

Query: 1   MKTTIQEHMLEFQMTLALAKARYLGHSSKHLDISSSNNGS---LKKGNGKDWILNRLNKN 60
           MKTT+QEH+LEFQM+LALAKARYLGHS KHL ISSSN+GS   +KKGNGKD ILNRLNKN
Sbjct: 1   MKTTLQEHILEFQMSLALAKARYLGHSLKHLRISSSNDGSQSLIKKGNGKDLILNRLNKN 60

Query: 61  EGKTDMLDDIIHALQEHVKLGGKISEKIKGKLGLGGRTLRVGGMRKIYKKLFPMNEEEKL 120
           EGKTDMLDD+IHA+QEH   GGKISEKIKGKLGLG R+LRVGGMRKIYKKLF MNEEEKL
Sbjct: 61  EGKTDMLDDVIHAVQEH---GGKISEKIKGKLGLGARSLRVGGMRKIYKKLFSMNEEEKL 120

Query: 121 LKVSQCYLSTTAGPLGGLLFLSTHKIAFCSAKSITVLSPPNGDNDYVRIHYKVVIPMEKV 180
           LKVSQCYLSTTAGPLGGLLFLSTHKIAFCSAKSIT  SPPNGDNDYVRIHYKVVIPMEKV
Sbjct: 121 LKVSQCYLSTTAGPLGGLLFLSTHKIAFCSAKSIT-FSPPNGDNDYVRIHYKVVIPMEKV 180

Query: 181 MGVNESDQKNVKTDSEKYIQIVTVDNLEFWFMGLLNNYQSIFNSLQVLVKTKL 230
           MGVNES+ KNVKTDSEKYIQIVTVDNLEFWFM  LNNYQSIFNSLQVLVKTKL
Sbjct: 181 MGVNESE-KNVKTDSEKYIQIVTVDNLEFWFMRFLNNYQSIFNSLQVLVKTKL 228

BLAST of CsGy5G019830 vs. ExPASy TrEMBL
Match: A0A5A7SSL2 (GEM-like protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00700 PE=3 SV=1)

HSP 1 Score: 305 bits (780), Expect = 3.13e-102
Identity = 168/230 (73.04%), Postives = 187/230 (81.30%), Query Frame = 0

Query: 3   TTIQEHMLEFQMTLALAKARYLGH-SSKHLDISSSNN----GSLKKGNGKDWILNRLNKN 62
           TTIQEHML FQM+ AL++ARYLGH  SKHL +SSS++     S+KK NGKD ILNRLNKN
Sbjct: 4   TTIQEHMLGFQMSFALSRARYLGHYPSKHLHVSSSDDRISQSSIKKYNGKDCILNRLNKN 63

Query: 63  EGKTDMLDDIIHALQEHVKLGGKISEKIKGKLGLGGRTLRVGGMRKIYKKLFPMNEEEKL 122
             KTD   +IIHAL+EHVKLG KISE +KGKL LG R LRVGG+RKIYKKLF MNEEEKL
Sbjct: 64  GKKTD---NIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMNEEEKL 123

Query: 123 LKVSQCYLSTTAGPLGGLLFLSTHKIAFCSAKSITVLSPPNGDNDYVRIHYKVVIPMEKV 182
           LKVSQCYLSTTAGPL GLLF+STHKIAFCS KSI + SP NGD  ++RIHYKVVIP EKV
Sbjct: 124 LKVSQCYLSTTAGPLAGLLFISTHKIAFCSDKSIKIASPNNGD--HIRIHYKVVIPKEKV 183

Query: 183 MGVNESDQKNVKTDSEKYIQIVTVDNLEFWFMGLLNNYQSIFNSLQVLVK 227
           M VNES+  NVK  SEKYIQI T+DN EFWFMG LN YQS FNSLQ + +
Sbjct: 184 MRVNESE--NVKKASEKYIQIETLDNFEFWFMGFLN-YQSTFNSLQEVAR 225

BLAST of CsGy5G019830 vs. ExPASy TrEMBL
Match: A0A1S3CNX8 (GEM-like protein 4 OS=Cucumis melo OX=3656 GN=LOC103503118 PE=3 SV=1)

HSP 1 Score: 305 bits (780), Expect = 3.13e-102
Identity = 168/230 (73.04%), Postives = 187/230 (81.30%), Query Frame = 0

Query: 3   TTIQEHMLEFQMTLALAKARYLGH-SSKHLDISSSNN----GSLKKGNGKDWILNRLNKN 62
           TTIQEHML FQM+ AL++ARYLGH  SKHL +SSS++     S+KK NGKD ILNRLNKN
Sbjct: 4   TTIQEHMLGFQMSFALSRARYLGHYPSKHLHVSSSDDRISQSSIKKYNGKDCILNRLNKN 63

Query: 63  EGKTDMLDDIIHALQEHVKLGGKISEKIKGKLGLGGRTLRVGGMRKIYKKLFPMNEEEKL 122
             KTD   +IIHAL+EHVKLG KISE +KGKL LG R LRVGG+RKIYKKLF MNEEEKL
Sbjct: 64  GKKTD---NIIHALREHVKLGAKISETVKGKLSLGARILRVGGVRKIYKKLFSMNEEEKL 123

Query: 123 LKVSQCYLSTTAGPLGGLLFLSTHKIAFCSAKSITVLSPPNGDNDYVRIHYKVVIPMEKV 182
           LKVSQCYLSTTAGPL GLLF+STHKIAFCS KSI + SP NGD  ++RIHYKVVIP EKV
Sbjct: 124 LKVSQCYLSTTAGPLAGLLFISTHKIAFCSDKSIKIASPNNGD--HIRIHYKVVIPKEKV 183

Query: 183 MGVNESDQKNVKTDSEKYIQIVTVDNLEFWFMGLLNNYQSIFNSLQVLVK 227
           M VNES+  NVK  SEKYIQI T+DN EFWFMG LN YQS FNSLQ + +
Sbjct: 184 MRVNESE--NVKKASEKYIQIETLDNFEFWFMGFLN-YQSTFNSLQEVAR 225

BLAST of CsGy5G019830 vs. ExPASy TrEMBL
Match: A0A1S3BEG1 (GEM-like protein 4 OS=Cucumis melo OX=3656 GN=LOC103488778 PE=3 SV=1)

HSP 1 Score: 301 bits (770), Expect = 8.17e-101
Identity = 167/226 (73.89%), Postives = 186/226 (82.30%), Query Frame = 0

Query: 1   MKTTIQEHMLEFQMTLALAKARYLGHSSKHLDISSSNNGS---LKKGNGKDWILNRLNKN 60
           MKTTIQEHML FQM+LAL++ARYLGH  K L +SSS++GS   +KK NGKD ILNRLN N
Sbjct: 1   MKTTIQEHMLGFQMSLALSRARYLGHPLKRLHLSSSDDGSQSSIKKYNGKDCILNRLNTN 60

Query: 61  EGKTDMLDDIIHALQEHVKLGGKISEKIKGKLGLGGRTLRVGGMRKIYKKLFPMNEEEKL 120
             +TD   +IIHAL+EHVKLGGKISE +KGKL LG + LRVGG+RKIYKKLF MNEEEKL
Sbjct: 61  GKRTD---NIIHALREHVKLGGKISEAVKGKLSLGAKILRVGGLRKIYKKLFSMNEEEKL 120

Query: 121 LKVSQCYLSTTAGPLGGLLFLSTHKIAFCSAKSITVLSPPNGDNDYVRIHYKVVIPMEKV 180
           LKVSQCYLSTTAGPL GLLF+STHKIAFCS KSI + SP NGD  ++RI YKVVIP EKV
Sbjct: 121 LKVSQCYLSTTAGPLAGLLFISTHKIAFCSDKSIKIASP-NGD--HIRILYKVVIPKEKV 180

Query: 181 MGVNESDQKNVKTDSEKYIQIVTVDNLEFWFMGLLNNYQSIFNSLQ 223
           M VNES+  NVK  SEKYIQI T+DN EFWFMG LN YQS FNSLQ
Sbjct: 181 MRVNESE--NVKKASEKYIQIETLDNFEFWFMGFLN-YQSTFNSLQ 217

BLAST of CsGy5G019830 vs. TAIR 10
Match: AT5G23370.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 168.7 bits (426), Expect = 5.5e-42
Identity = 93/197 (47.21%), Postives = 127/197 (64.47%), Query Frame = 0

Query: 27  SSKHLDISSSNNGSLKKGNGKDWILNRLNKNEGKTDMLDDIIHALQEHVKLGGKISEKIK 86
           S   L I +S+  S   G GK  +  + N         D   + +++  KLG K++E +K
Sbjct: 29  SINKLQIPTSSKFSFLTGKGKSMLRKKKN---------DSFTNGVRDQDKLGPKLTETVK 88

Query: 87  GKLGLGGRTLRVGGMRKIYKKLFPMNEEEKLLKVSQCYLSTTAGPLGGLLFLSTHKIAFC 146
            KL LG R L++GG+ KIYK+LF +++EEKL K  QCYLSTTAGP+ GLLF+S+ KIAFC
Sbjct: 89  RKLSLGARILQMGGLEKIYKRLFKVSDEEKLFKAYQCYLSTTAGPIAGLLFISSKKIAFC 148

Query: 147 SAKSITVLSPPNGDNDYVRIHYKVVIPMEKVMGVNESDQKNVKTDSEKYIQIVTVDNLEF 206
           S +SI V SP    N   R+HYKV IP+ K+ GVN+S  +N    S+KY+++VTVD  +F
Sbjct: 149 SERSIKVASPQGELN---RVHYKVSIPLCKINGVNQS--QNTTKPSQKYLEVVTVDGFDF 208

Query: 207 WFMGLLNNYQSIFNSLQ 224
           WFMG L +YQ  FN L+
Sbjct: 209 WFMGFL-SYQKAFNCLE 210

BLAST of CsGy5G019830 vs. TAIR 10
Match: AT5G23350.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 161.4 bits (407), Expect = 8.8e-40
Identity = 87/192 (45.31%), Postives = 126/192 (65.62%), Query Frame = 0

Query: 32  DISSSNNGSLKKGNGKDWILNRLNKNEGKTDMLDDIIHALQEHVKLGGKISEKIKGKLGL 91
           D +S NN  +   +   ++  +  K+  +    D   +  ++  KLG K++E +K KL L
Sbjct: 88  DPASINNHQIPTSSKVSYLTGK-GKSMLRKKKTDSFTNGARDQDKLGPKLTETVKRKLSL 147

Query: 92  GGRTLRVGGMRKIYKKLFPMNEEEKLLKVSQCYLSTTAGPLGGLLFLSTHKIAFCSAKSI 151
           G + L++GG+ KIYK+LF + ++EKL K  QCYLSTTAGP+ GLLF+S+ KIAFCS +SI
Sbjct: 148 GAKILQMGGLEKIYKRLFKVCDQEKLFKAYQCYLSTTAGPIAGLLFISSKKIAFCSERSI 207

Query: 152 TVLSPPNGDNDYVRIHYKVVIPMEKVMGVNESDQKNVKTDSEKYIQIVTVDNLEFWFMGL 211
            V SP        R+HYKV IP+ K+ GVN+S  +N K  S+KY++IVT+DN +FWFMG 
Sbjct: 208 KVASP---QGVLSRVHYKVSIPLCKINGVNQS--QNTKKPSQKYLEIVTIDNFDFWFMGF 267

Query: 212 LNNYQSIFNSLQ 224
           + +YQ  FN L+
Sbjct: 268 V-SYQKAFNCLE 272

BLAST of CsGy5G019830 vs. TAIR 10
Match: AT5G08350.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 161.0 bits (406), Expect = 1.1e-39
Identity = 82/164 (50.00%), Postives = 115/164 (70.12%), Query Frame = 0

Query: 60  KTDMLDDIIHALQEHVKLGGKISEKIKGKLGLGGRTLRVGGMRKIYKKLFPMNEEEKLLK 119
           K    D   + +++  K+  K++E +K KL LG R L+VGG+ KI+K+LF ++E EKL K
Sbjct: 52  KRKKTDGFTNGVRDQSKIRPKLTETVKRKLSLGARILQVGGLEKIFKRLFRVSEGEKLFK 111

Query: 120 VSQCYLSTTAGPLGGLLFLSTHKIAFCSAKSITVLSPPNGDNDYVRIHYKVVIPMEKVMG 179
           + QCYLSTTAGP+ GLLF+S+ K+AFCS +SI V SP     D +R+HYKV IP+ K+  
Sbjct: 112 MYQCYLSTTAGPIAGLLFISSKKMAFCSERSIKVDSP---QGDIIRVHYKVSIPLCKIDR 171

Query: 180 VNESDQKNVKTDSEKYIQIVTVDNLEFWFMGLLNNYQSIFNSLQ 224
           VN+S  +N K  S+KY+++VTVD  +FWFMG L +YQ  FN L+
Sbjct: 172 VNQS--QNTKKPSQKYLEVVTVDGFDFWFMGFL-SYQKAFNCLE 209

BLAST of CsGy5G019830 vs. TAIR 10
Match: AT5G23360.1 (GRAM domain-containing protein / ABA-responsive protein-related )

HSP 1 Score: 160.2 bits (404), Expect = 2.0e-39
Identity = 80/159 (50.31%), Postives = 112/159 (70.44%), Query Frame = 0

Query: 65  DDIIHALQEHVKLGGKISEKIKGKLGLGGRTLRVGGMRKIYKKLFPMNEEEKLLKVSQCY 124
           D   +  ++  KLG K++E +K KL LG + L++GG+ KIYK+LF + ++EKL K  QCY
Sbjct: 50  DSFTNGARDQDKLGPKLTETVKRKLSLGAKILQMGGLEKIYKRLFKVCDKEKLFKAYQCY 109

Query: 125 LSTTAGPLGGLLFLSTHKIAFCSAKSITVLSPPNGDNDYVRIHYKVVIPMEKVMGVNESD 184
           LSTT G + GLLF+S+ KIAFCS +SI V SP     D  R+HYKV IP+ K+ GVN+S 
Sbjct: 110 LSTTEGSIAGLLFISSKKIAFCSERSIKVTSP---QGDLTRVHYKVSIPLCKINGVNQS- 169

Query: 185 QKNVKTDSEKYIQIVTVDNLEFWFMGLLNNYQSIFNSLQ 224
            +N K  S++Y+++VTVDN +FWFMG + +YQ  FN L+
Sbjct: 170 -QNTKKPSQRYLEVVTVDNYDFWFMGFV-SYQKAFNCLE 202

BLAST of CsGy5G019830 vs. TAIR 10
Match: AT1G28200.1 (FH interacting protein 1 )

HSP 1 Score: 115.9 bits (289), Expect = 4.2e-26
Identity = 66/168 (39.29%), Postives = 102/168 (60.71%), Query Frame = 0

Query: 61  TDMLDDIIHALQEHVKLGGKISEKIKGKLGLGGRTLRVGGMRKIYKKLFPMNEEEKLLKV 120
           T   +D+     +H+K G  +++    ++  G + L  GG  K++K+ F    +EKLLK 
Sbjct: 96  TKKAEDLAGNFWQHLKTGPSVADAAVSRIAQGTKILAEGGYEKVFKQTFDCLPDEKLLKT 155

Query: 121 SQCYLSTTAGPLGGLLFLSTHKIAFCSAKSITVLSPPNGDNDYVRIHYKVVIPMEKVMGV 180
             CYLST+AGP+ G+++LSTHK+AF S      LS   G+   +  +YKVV+P  ++  V
Sbjct: 156 YACYLSTSAGPVLGVMYLSTHKLAFSSDNP---LSYKEGEQT-LWSYYKVVLPANQLKAV 215

Query: 181 NESDQKNVKTDSEKYIQIVTVDNLEFWFMGLLNNYQSIFNSLQVLVKT 229
           N S  + V T S+KYIQ++++DN EFWFMG +  Y+S   SLQ  V++
Sbjct: 216 NPSTSR-VNT-SDKYIQVISIDNHEFWFMGFV-TYESAVKSLQEAVQS 256

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FMW47.7e-4147.21Putative GEM-like protein 8 OS=Arabidopsis thaliana OX=3702 GN=At5g23370 PE=3 SV... [more]
Q9FMW61.2e-3845.31GEM-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=At5g23350 PE=2 SV=2[more]
Q9FTA01.6e-3850.00GEM-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=At5g08350 PE=2 SV=1[more]
Q9FMW52.7e-3850.31GEM-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=At5g23360 PE=2 SV=1[more]
Q9SE965.9e-2539.29GEM-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=FIP1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_011655498.13.00e-16199.13putative GEM-like protein 8 [Cucumis sativus] >KGN51564.1 hypothetical protein C... [more]
XP_008467050.18.33e-13889.27PREDICTED: putative GEM-like protein 8 [Cucumis melo][more]
XP_008465496.16.47e-10273.04PREDICTED: GEM-like protein 4 [Cucumis melo] >KAA0034082.1 GEM-like protein 4 [C... [more]
XP_011655499.12.37e-10172.73GEM-like protein 4 [Cucumis sativus] >KGN52179.2 hypothetical protein Csa_008572... [more]
XP_008445894.11.69e-10073.89PREDICTED: GEM-like protein 4 [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A0A0KRR91.45e-16199.13GRAM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G579020 PE=3 S... [more]
A0A1S3CST34.03e-13889.27putative GEM-like protein 8 OS=Cucumis melo OX=3656 GN=LOC103504485 PE=3 SV=1[more]
A0A5A7SSL23.13e-10273.04GEM-like protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00... [more]
A0A1S3CNX83.13e-10273.04GEM-like protein 4 OS=Cucumis melo OX=3656 GN=LOC103503118 PE=3 SV=1[more]
A0A1S3BEG18.17e-10173.89GEM-like protein 4 OS=Cucumis melo OX=3656 GN=LOC103488778 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G23370.15.5e-4247.21GRAM domain-containing protein / ABA-responsive protein-related [more]
AT5G23350.18.8e-4045.31GRAM domain-containing protein / ABA-responsive protein-related [more]
AT5G08350.11.1e-3950.00GRAM domain-containing protein / ABA-responsive protein-related [more]
AT5G23360.12.0e-3950.31GRAM domain-containing protein / ABA-responsive protein-related [more]
AT1G28200.14.2e-2639.29FH interacting protein 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004182GRAM domainSMARTSM00568gram2001ccoord: 103..184
e-value: 1.2E-14
score: 64.7
IPR004182GRAM domainPFAMPF02893GRAMcoord: 105..225
e-value: 5.3E-15
score: 55.3
IPR011993PH-like domain superfamilyGENE3D2.30.29.30coord: 101..228
e-value: 3.0E-17
score: 64.4
NoneNo IPR availablePANTHERPTHR31969:SF38GEM-LIKE PROTEIN 4coord: 1..228
IPR037848GEM-like proteinPANTHERPTHR31969GEM-LIKE PROTEIN 2coord: 1..228

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy5G019830.2CsGy5G019830.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004252 serine-type endopeptidase activity