CsGy5G017930 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy5G017930
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Descriptionsirohydrochlorin ferrochelatase, chloroplastic
LocationGy14Chr5: 24104951 .. 24107520 (+)
RNA-Seq ExpressionCsGy5G017930
SyntenyCsGy5G017930
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTAGGCCAATGATGCAATTGAATTTTTACCAACGACGTCGTTTTTTAAGTATCAGGTTCAAGGGCTGCCTCTTTCTCTGGCAAAGGATCAGGACCCATGTCTTCGTTTTCCATCACTCCTCAAATTTCACCCAAGAGGTTTCAATATCTCCTAAACCCTTGTTTTCTTTTCTATTTTTTCTTGATTTTTTTCCTCTCTAATGGCCGTCCATTTTGCCATTGAAGTTCTTCACCAGTGGAAACTAGAAGACTATTCTCAATCTGCGCGTCTCAGAGATCTTCAATTCCTGTTGGACGCCTTTCTAAATTCAGAAATTTCACCTCGCGGTTCCGCATGAGTATTGATAATGGAAGCTCTAATCGGATTGGTGCCGGAGATGCTGTGATTATCGTCGACCACGGCTCCCGTCGCCGGGAATCTAATCTCATGCTGAGTAAGTTAGGGTTTTAGAATTTGGAATTTTGGGTGAAGTTTTGAAGTTATTCGTTGGATAATTCTCTTGTGGTGATATTTTTTTCTTAAAAAAAAAAAAAAATTCAGATGAGTTCGTTGCTATGTTTAAGGATAAAACTGGTTACCCCATTGTGGAGCCTGCTCACATGGTATGTTCTTTTCAACTGTTTCTTATGCACAAAACTATTACTTTCATTCTTGAAGCAACATTATTTATTTTGGTAGTTGGTTTTTGGAAATCAATGCTGCATTTTAGTTAGGAGTGTAATGAAATGAGTTTGTAATGTAATAGGTCCAAAACCCATGTTTGGATTAAGCATTTCTGGTGGATTTGTAATACCAAACTTATGTCCTTTATGGAGTTTTCAATCACGGTTCCATTTCGCATCTCAATGCTTCTCAATTCTGGTTGCATTCTAGCTCTCCAAACATTTCCTTAAAAGTATTTTGGTCCCTCAATTATGGATTTAGTTCATTTATAAGTATTTTGGTCCCTCAATTATGGATTTAGTTCATTCTGGTTCCTATACTTCAATGTATATTTTGGAACAGAAATAAACATTTTGAAAATATAACTACCAAAATGTACCAAGTATAAGGACCAAACTAACATTTTAAACCTTTTGTTTGGTTCCATAATGGTGGTGCTAACCACTTTTTTTTTTGTTTTGTTCATGGCATATGTGTAAACCAATTAAACTATGTGTTAAGTTATTAACATTTGATCGATCAATAAAGACTAAAAGTAAACCATGATGAACAAAACTATAATAATTATTCAACATCAAGATTGAATCTTTTGATTTTTGAATAAATGTACAGTTTTGTGGATTTTTTTTTTCTACACTCTGAAATACAGCATAGTGAATAGAAAATCAAAATAAATACCGATACCATTCAAAATGAATACTGATTTGGAACTATTCTATCTCATCGTACTGCCAGTAGTTTCTTTGTCTTTTATTTCTCTCTCTTTTTGTTGCATTCAACACATGATAGAGTAACAAGTCACAGCCATGTCCAATGTACTTAGTTTCATTACTTTTTTTCTGAAACAACGTAGGAGCTGGCCGAACCATCCATTAAAGACTCATTCACATTGTGTGTTGAACAAGGGGCCAAACGTATCATCGTCAGCCCGTTCTTTCTTTTCCCCGGTCGACATTGGCAACAGGTATGTAACTCACTGTAGGACACTTGATTTGATGAAATGAACTGGTGGATTGATGAGAGCAATACGTGAAACTTTGCAGGATATTCCTTCCTTAACAGCTGAAGCAGCAAAGGATCACCCAGGAATATCGTATATAATAACTGCACCTCTCGGTCTTCACGAACAACTTGTGGTAAGTATTTCTAAAGCATATTTTTTTCTAGTATCTAATAGAAAAAGGATGAAAATATCCATTGACATGAAATCAAAGGTTTGACATCAATATTAAATATACGTCATTAATAAATGCTTATAAAACAAAAAGAAATGTTGTCATTGTTTAATCTAATATGAATAGAAACGTTAATAACAATATCCACAAGGAAAACAATTTGATTGTTAATCTAAATAAATTTTATAAGTCATTCGATATCAATATTTAATCAAAATATCTGTTGATATATCTGTAAAATTGAAATCATGTTGCGTATGATAAAAGAAAATGACTATTTTTTTAGTATTGAACTATGAGATGGTTCTGTTGGCAGGATGTTGTGAACGATAGAATAAATTACTGCTTAAGCCATGCTGCTGGAGTGTCAGACGAATGTGAAGTTTGTGCTGGCACAGGCAAATGTAGATTTAATTAGTGTATGAAAGTAGAGACTTGGGATGAAATCGCTCGGTTTTTCGACGACTTTCATTTGAATACCATTCAATGGCTTGGCAGTTGAAGGCTTGAGAATCTATTTATCTTGTTTTGTATCAATACTTTGTAAAATTTGAGCTCTTTGGTTGTTTTTGTATATTATATCTTCACATATGACTACGGTCTACAACAATATGCACAATAATATTAGTTCCCAAAAATGATTTTGAGAACAGTTCGAGATTAGTGATAGAAAATCAGAGCTTTTTCATGAACCTACCTATCACTAAAAACTTGCTTTACCCAATTCCAC

mRNA sequence

ATTTAGGCCAATGATGCAATTGAATTTTTACCAACGACGTCGTTTTTTAAGTATCAGGTTCAAGGGCTGCCTCTTTCTCTGGCAAAGGATCAGGACCCATGTCTTCGTTTTCCATCACTCCTCAAATTTCACCCAAGAGTTCTTCACCAGTGGAAACTAGAAGACTATTCTCAATCTGCGCGTCTCAGAGATCTTCAATTCCTGTTGGACGCCTTTCTAAATTCAGAAATTTCACCTCGCGGTTCCGCATGAGTATTGATAATGGAAGCTCTAATCGGATTGGTGCCGGAGATGCTGTGATTATCGTCGACCACGGCTCCCGTCGCCGGGAATCTAATCTCATGCTGAATGAGTTCGTTGCTATGTTTAAGGATAAAACTGGTTACCCCATTGTGGAGCCTGCTCACATGGAGCTGGCCGAACCATCCATTAAAGACTCATTCACATTGTGTGTTGAACAAGGGGCCAAACGTATCATCGTCAGCCCGTTCTTTCTTTTCCCCGGTCGACATTGGCAACAGGATATTCCTTCCTTAACAGCTGAAGCAGCAAAGGATCACCCAGGAATATCGTATATAATAACTGCACCTCTCGGTCTTCACGAACAACTTGTGGATGTTGTGAACGATAGAATAAATTACTGCTTAAGCCATGCTGCTGGAGTGTCAGACGAATGTGAAGTTTGTGCTGGCACAGGCAAATGTAGATTTAATTAGTGTATGAAAGTAGAGACTTGGGATGAAATCGCTCGGTTTTTCGACGACTTTCATTTGAATACCATTCAATGGCTTGGCAGTTGAAGGCTTGAGAATCTATTTATCTTGTTTTGTATCAATACTTTGTAAAATTTGAGCTCTTTGGTTGTTTTTGTATATTATATCTTCACATATGACTACGGTCTACAACAATATGCACAATAATATTAGTTCCCAAAAATGATTTTGAGAACAGTTCGAGATTAGTGATAGAAAATCAGAGCTTTTTCATGAACCTACCTATCACTAAAAACTTGCTTTACCCAATTCCAC

Coding sequence (CDS)

ATGTCTTCGTTTTCCATCACTCCTCAAATTTCACCCAAGAGTTCTTCACCAGTGGAAACTAGAAGACTATTCTCAATCTGCGCGTCTCAGAGATCTTCAATTCCTGTTGGACGCCTTTCTAAATTCAGAAATTTCACCTCGCGGTTCCGCATGAGTATTGATAATGGAAGCTCTAATCGGATTGGTGCCGGAGATGCTGTGATTATCGTCGACCACGGCTCCCGTCGCCGGGAATCTAATCTCATGCTGAATGAGTTCGTTGCTATGTTTAAGGATAAAACTGGTTACCCCATTGTGGAGCCTGCTCACATGGAGCTGGCCGAACCATCCATTAAAGACTCATTCACATTGTGTGTTGAACAAGGGGCCAAACGTATCATCGTCAGCCCGTTCTTTCTTTTCCCCGGTCGACATTGGCAACAGGATATTCCTTCCTTAACAGCTGAAGCAGCAAAGGATCACCCAGGAATATCGTATATAATAACTGCACCTCTCGGTCTTCACGAACAACTTGTGGATGTTGTGAACGATAGAATAAATTACTGCTTAAGCCATGCTGCTGGAGTGTCAGACGAATGTGAAGTTTGTGCTGGCACAGGCAAATGTAGATTTAATTAG

Protein sequence

MSSFSITPQISPKSSSPVETRRLFSICASQRSSIPVGRLSKFRNFTSRFRMSIDNGSSNRIGAGDAVIIVDHGSRRRESNLMLNEFVAMFKDKTGYPIVEPAHMELAEPSIKDSFTLCVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRINYCLSHAAGVSDECEVCAGTGKCRFN*
Homology
[Close]
BLAST of CsGy5G017930 vs. ExPASy Swiss-Prot
Match: Q84JH7 (Sirohydrochlorin ferrochelatase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SIRB PE=1 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 3.8e-63
Identity = 122/205 (59.51%), Postives = 147/205 (71.71%), Query Frame = 0 

Query: 2   SSFSITPQISPKSSSPVETRRLFSICAS---QRSSIPVGRLSKFRNFTSRFRMSIDNGSS 61
           S+     ++SP S     T R +++  S   ++  +  GR  +           + NG  
Sbjct: 20  SNLRANNRVSPSSCQITRTNRSWALPVSFKVEKFQLQRGRRRRGSPCFGESEGLVKNG-- 79

Query: 62  NRIGAGDAVIIVDHGSRRRESNLMLNEFVAMFKDKTGYPIVEPAHMELAEPSIKDSFTLC 121
             IG  D +IIVDHGSRRRESNLML EFV MFK+KTGYPIVEPAHMELAEPSIKD+F+LC
Sbjct: 80  --IGDADGIIIVDHGSRRRESNLMLEEFVKMFKEKTGYPIVEPAHMELAEPSIKDAFSLC 139

Query: 122 VEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR 181
           V+QGAKR++VSPFFLFPGRHW  DIPSLTA+AAK+  GISY+ITAPLG H  L+DVVNDR
Sbjct: 140 VQQGAKRVVVSPFFLFPGRHWHTDIPSLTADAAKEFSGISYLITAPLGPHNLLLDVVNDR 199

Query: 182 INYCLSHAAGVSDECEVCAGTGKCR 204
           I +CLSH  G +DEC VCAGT KC+
Sbjct: 200 IQHCLSHVEGDADECLVCAGTNKCK 220

[Close]
BLAST of CsGy5G017930 vs. ExPASy Swiss-Prot
Match: P61817 (Sirohydrochlorin ferrochelatase OS=Bacillus megaterium OX=1404 GN=sirB PE=3 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 2.6e-16
Identity = 42/116 (36.21%), Postives = 62/116 (53.45%), Query Frame = 0 

Query: 65  DAVIIVDHGSRRRESNLMLNEFVAMFKDKTGYPIVEPAHMELAEPSIKDSFTLCVEQGAK 124
           DAV+ V HGSR +E       F+   K     PI E   +ELA P+I+  F  C+EQGA 
Sbjct: 13  DAVLYVCHGSRVKEGADQAVAFIERCKKNLDVPIQEVCFLELASPTIEQGFEACIEQGAT 72

Query: 125 RIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRIN 181
           RI + P  L    H + DIP    +  + +P +  +   P G+ E++VD++ +RIN
Sbjct: 73  RIAIVPLLLLTAAHAKHDIPEEIQKVYERYPQVEVLYGEPFGVDERIVDILVERIN 128

[Close]
BLAST of CsGy5G017930 vs. ExPASy Swiss-Prot
Match: P61819 (Sirohydrochlorin cobaltochelatase OS=Methanococcus maripaludis (strain S2 / LL) OX=267377 GN=cbiX PE=3 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 1.2e-13
Identity = 42/141 (29.79%), Postives = 67/141 (47.52%), Query Frame = 0 

Query: 65  DAVIIVDHGSRRRESNLMLNEFVAMFKDKTGYPIVEPAHMELAEPSIKDSFTLCVEQGAK 124
           +A+++V HGSR   S  ++ E     K +  Y IVE   ME  EP+I  +    ++ GAK
Sbjct: 2   EALVLVGHGSRLPHSKNVVMEVAEKIKARNIYDIVEVGMMEFNEPTIPQTIKKVIDAGAK 61

Query: 125 RIIVSPFFLFPGRHWQQDIPSLTA------EAAKDH-------------------PGISY 181
           ++IV+P FL PG H ++DIP +        E    H                    G+  
Sbjct: 62  KVIVTPVFLAPGNHTERDIPKILGIYEGDDEGGHHHHHDHEHHHHHHDTTAVEIPEGVEL 121

[Close]
BLAST of CsGy5G017930 vs. ExPASy Swiss-Prot
Match: Q58380 (Sirohydrochlorin cobaltochelatase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=cbiX PE=1 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 4.7e-13
Identity = 44/138 (31.88%), Postives = 66/138 (47.83%), Query Frame = 0 

Query: 65  DAVIIVDHGSRRRESNLMLNEFVAMFKDKTGYPIVEPAHMELAEPSIKDSFTLCVEQGAK 124
           +A+++V HGSR   S  +L +     K++  +PIVE   ME +EP+I  +    +EQGAK
Sbjct: 2   EALVLVGHGSRLPYSKELLVKLAEKVKERNLFPIVEIGLMEFSEPTIPQAVKKAIEQGAK 61

Query: 125 RIIVSPFFLFPGRHWQQDIPSLTAEAAKDH------------------------PGISYI 179
           RIIV P FL  G H  +DIP L      +H                          +  I
Sbjct: 62  RIIVVPVFLAHGIHTTRDIPRLLGLIEDNHEHHHEHSHHHHHHHHHEHEKLEIPEDVEII 121

[Close]
BLAST of CsGy5G017930 vs. ExPASy Swiss-Prot
Match: A6UWT5 (Sirohydrochlorin cobaltochelatase OS=Methanococcus aeolicus (strain ATCC BAA-1280 / DSM 17508 / OCM 812 / Nankai-3) OX=419665 GN=cbiX PE=3 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 8.8e-12
Identity = 41/138 (29.71%), Postives = 66/138 (47.83%), Query Frame = 0 

Query: 65  DAVIIVDHGSRRRESNLMLNEFVAMFKDKTGYPIVEPAHMELAEPSIKDSFTLCVEQGAK 124
           +A++++ HGSR   S  ++ +     K+K  Y IVE   ME  EP+I  +    + +GAK
Sbjct: 2   EALVLLGHGSRLPYSKEIVGKVAEKIKEKNIYDIVEIGMMEFNEPTIPQTINKVIAEGAK 61

Query: 125 RIIVSPFFLFPGRHWQQDIPSLTA--EAAKDH----------------------PGISYI 179
           +II+ P FL  G H ++DIP +    E  + H                       G+  I
Sbjct: 62  KIIIVPVFLAHGNHTKRDIPQILGLIECEEHHHEGEGGHHHHHHHHHGEKIEVPEGVEII 121

[Close]
BLAST of CsGy5G017930 vs. NCBI nr
Match: XP_031742215.1 (sirohydrochlorin ferrochelatase, chloroplastic [Cucumis sativus] >KGN51370.1 hypothetical protein Csa_008200 [Cucumis sativus])

HSP 1 Score: 412 bits (1060), Expect = 2.61e-145
Identity = 205/205 (100.00%), Postives = 205/205 (100.00%), Query Frame = 0 

Query: 1   MSSFSITPQISPKSSSPVETRRLFSICASQRSSIPVGRLSKFRNFTSRFRMSIDNGSSNR 60
           MSSFSITPQISPKSSSPVETRRLFSICASQRSSIPVGRLSKFRNFTSRFRMSIDNGSSNR
Sbjct: 1   MSSFSITPQISPKSSSPVETRRLFSICASQRSSIPVGRLSKFRNFTSRFRMSIDNGSSNR 60

Query: 61  IGAGDAVIIVDHGSRRRESNLMLNEFVAMFKDKTGYPIVEPAHMELAEPSIKDSFTLCVE 120
           IGAGDAVIIVDHGSRRRESNLMLNEFVAMFKDKTGYPIVEPAHMELAEPSIKDSFTLCVE
Sbjct: 61  IGAGDAVIIVDHGSRRRESNLMLNEFVAMFKDKTGYPIVEPAHMELAEPSIKDSFTLCVE 120

Query: 121 QGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRIN 180
           QGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRIN
Sbjct: 121 QGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRIN 180

Query: 181 YCLSHAAGVSDECEVCAGTGKCRFN 205
           YCLSHAAGVSDECEVCAGTGKCRFN
Sbjct: 181 YCLSHAAGVSDECEVCAGTGKCRFN 205

[Close]
BLAST of CsGy5G017930 vs. NCBI nr
Match: KAA0037556.1 (sirohydrochlorin ferrochelatase [Cucumis melo var. makuwa] >TYK03116.1 sirohydrochlorin ferrochelatase [Cucumis melo var. makuwa])

HSP 1 Score: 384 bits (986), Expect = 5.00e-134
Identity = 188/205 (91.71%), Postives = 197/205 (96.10%), Query Frame = 0 

Query: 1   MSSFSITPQISPKSSSPVETRRLFSICASQRSSIPVGRLSKFRNFTSRFRMSIDNGSSNR 60
           MSS S+TPQISPKS SPVETR  ++ICA QRS IP+GRLSKFRNFTSR RMSIDNGSSNR
Sbjct: 1   MSSLSVTPQISPKSPSPVETRSPYTICAFQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNR 60

Query: 61  IGAGDAVIIVDHGSRRRESNLMLNEFVAMFKDKTGYPIVEPAHMELAEPSIKDSFTLCVE 120
           IGAGDAVIIVDHGSRRRESNLMLNEFVAMF+DKTGYPIVEPAHMELAEPSI+DSFT CVE
Sbjct: 61  IGAGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVE 120

Query: 121 QGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRIN 180
           QGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHE+LVDVVNDRIN
Sbjct: 121 QGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDRIN 180

Query: 181 YCLSHAAGVSDECEVCAGTGKCRFN 205
           +CLSHAAGV+DECEVCAGTGKCRFN
Sbjct: 181 HCLSHAAGVADECEVCAGTGKCRFN 205

[Close]
BLAST of CsGy5G017930 vs. NCBI nr
Match: XP_008458790.1 (PREDICTED: sirohydrochlorin ferrochelatase, chloroplastic [Cucumis melo])

HSP 1 Score: 384 bits (986), Expect = 5.00e-134
Identity = 188/205 (91.71%), Postives = 197/205 (96.10%), Query Frame = 0 

Query: 1   MSSFSITPQISPKSSSPVETRRLFSICASQRSSIPVGRLSKFRNFTSRFRMSIDNGSSNR 60
           MSS S+TPQISPKS SPVETR  ++ICASQRS IP+GRLSKFRNFTSR RMSIDNGSSNR
Sbjct: 1   MSSLSVTPQISPKSPSPVETRSPYTICASQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNR 60

Query: 61  IGAGDAVIIVDHGSRRRESNLMLNEFVAMFKDKTGYPIVEPAHMELAEPSIKDSFTLCVE 120
           IGAGDAVIIVDHGSRRRESN MLNEFVAMF+DKTGYPIVEPAHMELAEPSI+DSFT CVE
Sbjct: 61  IGAGDAVIIVDHGSRRRESNRMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVE 120

Query: 121 QGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRIN 180
           QGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHE+LVDVVNDRIN
Sbjct: 121 QGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDRIN 180

Query: 181 YCLSHAAGVSDECEVCAGTGKCRFN 205
           +CLSHAAGV+DECEVCAGTGKCRFN
Sbjct: 181 HCLSHAAGVADECEVCAGTGKCRFN 205

[Close]
BLAST of CsGy5G017930 vs. NCBI nr
Match: XP_038890885.1 (sirohydrochlorin ferrochelatase, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 363 bits (931), Expect = 5.15e-125
Identity = 183/210 (87.14%), Postives = 197/210 (93.81%), Query Frame = 0 

Query: 1   MSSFSI-TPQISPKSSSPVETRRLFSICASQRSSIPVGRLSKFRNFTSRFRMSIDNGSSN 60
           M+S SI TPQIS KSSS VETRR ++ICASQ+S I + RLS+FRNFTSRFRMS+DNGSSN
Sbjct: 37  MTSLSIVTPQISLKSSSAVETRRKYTICASQKSPILIRRLSRFRNFTSRFRMSVDNGSSN 96

Query: 61  R----IGAGDAVIIVDHGSRRRESNLMLNEFVAMFKDKTGYPIVEPAHMELAEPSIKDSF 120
           R    IGAGDAVIIVDHGSRRRESNLMLNEFVAMF+DKTGYPIVEPAHMELAEPSI+DSF
Sbjct: 97  RSGDRIGAGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSF 156

Query: 121 TLCVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVV 180
            LCVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHE+LVDVV
Sbjct: 157 ALCVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVV 216

Query: 181 NDRINYCLSHAAGVSDECEVCAGTGKCRFN 205
           NDRI++CLSHAAGV++ECEVCAGTGKCRFN
Sbjct: 217 NDRISHCLSHAAGVAEECEVCAGTGKCRFN 246

[Close]
BLAST of CsGy5G017930 vs. NCBI nr
Match: XP_022993758.1 (sirohydrochlorin ferrochelatase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 353 bits (905), Expect = 1.19e-121
Identity = 174/207 (84.06%), Postives = 190/207 (91.79%), Query Frame = 0 

Query: 1   MSSFSITPQISPKSSSPVETRRLFSICASQRSSI--PVGRLSKFRNFTSRFRMSIDNGSS 60
           M+S SI PQI+ KS S  ETR+ ++ICASQ+SSI   +GR S  RNF+SRFRMS+DNG S
Sbjct: 1   MTSLSIPPQITVKSPSAGETRKTYTICASQKSSILIGIGRSSTLRNFSSRFRMSVDNGGS 60

Query: 61  NRIGAGDAVIIVDHGSRRRESNLMLNEFVAMFKDKTGYPIVEPAHMELAEPSIKDSFTLC 120
           NRIGAGDAVIIVDHGSRRRESNLMLNEFVAMF+DKTGYPIVEPAHMELAEPSI+DSF  C
Sbjct: 61  NRIGAGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASC 120

Query: 121 VEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR 180
           VEQGAKRI+VSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR
Sbjct: 121 VEQGAKRIVVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR 180

Query: 181 INYCLSHAAGVSDECEVCAGTGKCRFN 205
           I++CLSHAAGV +ECEVCAGTGKC+FN
Sbjct: 181 ISHCLSHAAGVVEECEVCAGTGKCKFN 207

[Close]
BLAST of CsGy5G017930 vs. ExPASy TrEMBL
Match: A0A0A0KP17 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G523150 PE=4 SV=1)

HSP 1 Score: 412 bits (1060), Expect = 1.26e-145
Identity = 205/205 (100.00%), Postives = 205/205 (100.00%), Query Frame = 0 

Query: 1   MSSFSITPQISPKSSSPVETRRLFSICASQRSSIPVGRLSKFRNFTSRFRMSIDNGSSNR 60
           MSSFSITPQISPKSSSPVETRRLFSICASQRSSIPVGRLSKFRNFTSRFRMSIDNGSSNR
Sbjct: 1   MSSFSITPQISPKSSSPVETRRLFSICASQRSSIPVGRLSKFRNFTSRFRMSIDNGSSNR 60

Query: 61  IGAGDAVIIVDHGSRRRESNLMLNEFVAMFKDKTGYPIVEPAHMELAEPSIKDSFTLCVE 120
           IGAGDAVIIVDHGSRRRESNLMLNEFVAMFKDKTGYPIVEPAHMELAEPSIKDSFTLCVE
Sbjct: 61  IGAGDAVIIVDHGSRRRESNLMLNEFVAMFKDKTGYPIVEPAHMELAEPSIKDSFTLCVE 120

Query: 121 QGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRIN 180
           QGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRIN
Sbjct: 121 QGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRIN 180

Query: 181 YCLSHAAGVSDECEVCAGTGKCRFN 205
           YCLSHAAGVSDECEVCAGTGKCRFN
Sbjct: 181 YCLSHAAGVSDECEVCAGTGKCRFN 205

[Close]
BLAST of CsGy5G017930 vs. ExPASy TrEMBL
Match: A0A5D3BY55 (Sirohydrochlorin ferrochelatase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold374G00520 PE=4 SV=1)

HSP 1 Score: 384 bits (986), Expect = 2.42e-134
Identity = 188/205 (91.71%), Postives = 197/205 (96.10%), Query Frame = 0 

Query: 1   MSSFSITPQISPKSSSPVETRRLFSICASQRSSIPVGRLSKFRNFTSRFRMSIDNGSSNR 60
           MSS S+TPQISPKS SPVETR  ++ICA QRS IP+GRLSKFRNFTSR RMSIDNGSSNR
Sbjct: 1   MSSLSVTPQISPKSPSPVETRSPYTICAFQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNR 60

Query: 61  IGAGDAVIIVDHGSRRRESNLMLNEFVAMFKDKTGYPIVEPAHMELAEPSIKDSFTLCVE 120
           IGAGDAVIIVDHGSRRRESNLMLNEFVAMF+DKTGYPIVEPAHMELAEPSI+DSFT CVE
Sbjct: 61  IGAGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVE 120

Query: 121 QGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRIN 180
           QGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHE+LVDVVNDRIN
Sbjct: 121 QGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDRIN 180

Query: 181 YCLSHAAGVSDECEVCAGTGKCRFN 205
           +CLSHAAGV+DECEVCAGTGKCRFN
Sbjct: 181 HCLSHAAGVADECEVCAGTGKCRFN 205

[Close]
BLAST of CsGy5G017930 vs. ExPASy TrEMBL
Match: A0A1S3C8S9 (sirohydrochlorin ferrochelatase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103498093 PE=4 SV=1)

HSP 1 Score: 384 bits (986), Expect = 2.42e-134
Identity = 188/205 (91.71%), Postives = 197/205 (96.10%), Query Frame = 0 

Query: 1   MSSFSITPQISPKSSSPVETRRLFSICASQRSSIPVGRLSKFRNFTSRFRMSIDNGSSNR 60
           MSS S+TPQISPKS SPVETR  ++ICASQRS IP+GRLSKFRNFTSR RMSIDNGSSNR
Sbjct: 1   MSSLSVTPQISPKSPSPVETRSPYTICASQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNR 60

Query: 61  IGAGDAVIIVDHGSRRRESNLMLNEFVAMFKDKTGYPIVEPAHMELAEPSIKDSFTLCVE 120
           IGAGDAVIIVDHGSRRRESN MLNEFVAMF+DKTGYPIVEPAHMELAEPSI+DSFT CVE
Sbjct: 61  IGAGDAVIIVDHGSRRRESNRMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVE 120

Query: 121 QGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRIN 180
           QGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHE+LVDVVNDRIN
Sbjct: 121 QGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDRIN 180

Query: 181 YCLSHAAGVSDECEVCAGTGKCRFN 205
           +CLSHAAGV+DECEVCAGTGKCRFN
Sbjct: 181 HCLSHAAGVADECEVCAGTGKCRFN 205

[Close]
BLAST of CsGy5G017930 vs. ExPASy TrEMBL
Match: A0A6J1K384 (sirohydrochlorin ferrochelatase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111489668 PE=4 SV=1)

HSP 1 Score: 353 bits (905), Expect = 5.77e-122
Identity = 174/207 (84.06%), Postives = 190/207 (91.79%), Query Frame = 0 

Query: 1   MSSFSITPQISPKSSSPVETRRLFSICASQRSSI--PVGRLSKFRNFTSRFRMSIDNGSS 60
           M+S SI PQI+ KS S  ETR+ ++ICASQ+SSI   +GR S  RNF+SRFRMS+DNG S
Sbjct: 1   MTSLSIPPQITVKSPSAGETRKTYTICASQKSSILIGIGRSSTLRNFSSRFRMSVDNGGS 60

Query: 61  NRIGAGDAVIIVDHGSRRRESNLMLNEFVAMFKDKTGYPIVEPAHMELAEPSIKDSFTLC 120
           NRIGAGDAVIIVDHGSRRRESNLMLNEFVAMF+DKTGYPIVEPAHMELAEPSI+DSF  C
Sbjct: 61  NRIGAGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASC 120

Query: 121 VEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR 180
           VEQGAKRI+VSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR
Sbjct: 121 VEQGAKRIVVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR 180

Query: 181 INYCLSHAAGVSDECEVCAGTGKCRFN 205
           I++CLSHAAGV +ECEVCAGTGKC+FN
Sbjct: 181 ISHCLSHAAGVVEECEVCAGTGKCKFN 207

[Close]
BLAST of CsGy5G017930 vs. ExPASy TrEMBL
Match: A0A6J1FHT5 (sirohydrochlorin ferrochelatase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111445470 PE=4 SV=1)

HSP 1 Score: 347 bits (890), Expect = 1.11e-119
Identity = 172/207 (83.09%), Postives = 189/207 (91.30%), Query Frame = 0 

Query: 1   MSSFSITPQISPKSSSPVETRRLFSICASQRSSI--PVGRLSKFRNFTSRFRMSIDNGSS 60
           M+S SI  QI+ KS S  ETR+ ++ICASQ+SSI   VGR S  RNF+SRFRMS+DNG +
Sbjct: 1   MTSLSIPSQITVKSPSAGETRKTYTICASQKSSILIGVGRSSTLRNFSSRFRMSVDNGGA 60

Query: 61  NRIGAGDAVIIVDHGSRRRESNLMLNEFVAMFKDKTGYPIVEPAHMELAEPSIKDSFTLC 120
           +RIGAGDAVIIVDHGSRRRESNLMLNEFVAMF+DKTGYPIVEPAHMELAEPSI+DSF  C
Sbjct: 61  DRIGAGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASC 120

Query: 121 VEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR 180
           VEQGAKRI+VSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR
Sbjct: 121 VEQGAKRIVVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR 180

Query: 181 INYCLSHAAGVSDECEVCAGTGKCRFN 205
           I++CLSHAAGV +ECEVCAGTGKC+FN
Sbjct: 181 ISHCLSHAAGVVEECEVCAGTGKCKFN 207

[Close]
BLAST of CsGy5G017930 vs. TAIR 10
Match: AT1G50170.1 (sirohydrochlorin ferrochelatase B )

HSP 1 Score: 242.7 bits (618), Expect = 2.7e-64
Identity = 122/205 (59.51%), Postives = 147/205 (71.71%), Query Frame = 0 

Query: 2   SSFSITPQISPKSSSPVETRRLFSICAS---QRSSIPVGRLSKFRNFTSRFRMSIDNGSS 61
           S+     ++SP S     T R +++  S   ++  +  GR  +           + NG  
Sbjct: 20  SNLRANNRVSPSSCQITRTNRSWALPVSFKVEKFQLQRGRRRRGSPCFGESEGLVKNG-- 79

Query: 62  NRIGAGDAVIIVDHGSRRRESNLMLNEFVAMFKDKTGYPIVEPAHMELAEPSIKDSFTLC 121
             IG  D +IIVDHGSRRRESNLML EFV MFK+KTGYPIVEPAHMELAEPSIKD+F+LC
Sbjct: 80  --IGDADGIIIVDHGSRRRESNLMLEEFVKMFKEKTGYPIVEPAHMELAEPSIKDAFSLC 139

Query: 122 VEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDR 181
           V+QGAKR++VSPFFLFPGRHW  DIPSLTA+AAK+  GISY+ITAPLG H  L+DVVNDR
Sbjct: 140 VQQGAKRVVVSPFFLFPGRHWHTDIPSLTADAAKEFSGISYLITAPLGPHNLLLDVVNDR 199

Query: 182 INYCLSHAAGVSDECEVCAGTGKCR 204
           I +CLSH  G +DEC VCAGT KC+
Sbjct: 200 IQHCLSHVEGDADECLVCAGTNKCK 220

The following BLAST results are available for this feature:
BLAST of CsGy5G017930 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q84JH73.8e-6359.51Sirohydrochlorin ferrochelatase, chloroplastic OS=Arabidopsis thaliana OX=3702 G... [more]
P618172.6e-1636.21Sirohydrochlorin ferrochelatase OS=Bacillus megaterium OX=1404 GN=sirB PE=3 SV=1[more]
P618191.2e-1329.79Sirohydrochlorin cobaltochelatase OS=Methanococcus maripaludis (strain S2 / LL) ... [more]
Q583804.7e-1331.88Sirohydrochlorin cobaltochelatase OS=Methanocaldococcus jannaschii (strain ATCC ... [more]
A6UWT58.8e-1229.71Sirohydrochlorin cobaltochelatase OS=Methanococcus aeolicus (strain ATCC BAA-128... [more]
BLAST of CsGy5G017930 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_031742215.12.61e-145100.00sirohydrochlorin ferrochelatase, chloroplastic [Cucumis sativus] >KGN51370.1 hyp... [more]
KAA0037556.15.00e-13491.71sirohydrochlorin ferrochelatase [Cucumis melo var. makuwa] >TYK03116.1 sirohydro... [more]
XP_008458790.15.00e-13491.71PREDICTED: sirohydrochlorin ferrochelatase, chloroplastic [Cucumis melo][more]
XP_038890885.15.15e-12587.14sirohydrochlorin ferrochelatase, chloroplastic isoform X1 [Benincasa hispida][more]
XP_022993758.11.19e-12184.06sirohydrochlorin ferrochelatase, chloroplastic-like [Cucurbita maxima][more]
BLAST of CsGy5G017930 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KP171.26e-145100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G523150 PE=4 SV=1[more]
A0A5D3BY552.42e-13491.71Sirohydrochlorin ferrochelatase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A1S3C8S92.42e-13491.71sirohydrochlorin ferrochelatase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A6J1K3845.77e-12284.06sirohydrochlorin ferrochelatase, chloroplastic-like OS=Cucurbita maxima OX=3661 ... [more]
A0A6J1FHT51.11e-11983.09sirohydrochlorin ferrochelatase, chloroplastic-like OS=Cucurbita moschata OX=366... [more]
BLAST of CsGy5G017930 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G50170.12.7e-6459.51sirohydrochlorin ferrochelatase B [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002762Sirohydrochlorin cobaltochelatase CbiX-likePFAMPF01903CbiXcoord: 72..176
e-value: 2.2E-29
score: 101.9
NoneNo IPR availableGENE3D3.40.50.1400coord: 63..185
e-value: 2.0E-33
score: 117.3
NoneNo IPR availablePANTHERPTHR33542SIROHYDROCHLORIN FERROCHELATASE, CHLOROPLASTICcoord: 58..202
NoneNo IPR availablePANTHERPTHR33542:SF3SIROHYDROCHLORIN FERROCHELATASE, CHLOROPLASTICcoord: 58..202
NoneNo IPR availableCDDcd03416CbiX_SirB_Ncoord: 66..166
e-value: 2.17811E-38
score: 126.147
NoneNo IPR availableSUPERFAMILY53800Chelatasecoord: 65..180

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy5G017930.2CsGy5G017930.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006979 response to oxidative stress
biological_process GO:0019354 siroheme biosynthetic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0042802 identical protein binding
molecular_function GO:0051536 iron-sulfur cluster binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0051266 sirohydrochlorin ferrochelatase activity
molecular_function GO:0016829 lyase activity