CsGy5G013370 (gene) Cucumber (Gy14) v2.1

BLAST of CsGy5G013370 vs. ExPASy Swiss-Prot
Match: M4IQQ5 (2-methylpropanoate--CoA ligase CCL4 OS=Humulus lupulus OX=3486 GN=CCL4 PE=1 SV=1)

HSP 1 Score: 811.2 bits (2094), Expect = 7.4e-234
Identity = 378/548 (68.98%), Postives = 466/548 (85.04%), Query Frame = 0

Query: 22  MEHLKPTPANSSPLTPIGFLERASLIYADSPSIIYSSTTFTWSQTYRRCLRLASSLSSIG 81
           ME LKP PA+SSPLTP+GFLERA+ +Y D  S++Y + ++TWSQT+RRCL LASS++S+G
Sbjct: 1   MEDLKPRPASSSPLTPLGFLERAATVYGDCTSVVYDAVSYTWSQTHRRCLCLASSIASLG 60

Query: 82  IHKGHVVSVIAPNTPPMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 141
           I  GHVVSV+APN P MYEL FAVPMAGAIL+ +NLRLDARTIS+ L HSESKL+FVD  
Sbjct: 61  IENGHVVSVLAPNVPQMYELHFAVPMAGAILNAVNLRLDARTISILLHHSESKLIFVDHL 120

Query: 142 SSGLISDALALFPPEAKRPLLVLISDDAAVEDSASSVAIGGDVVDLYEDMVQKGDPEFRW 201
           S  LI +A+ALFP +A  P LV ++D++   +S +S  +G +    Y+D++ +GDP+F+W
Sbjct: 121 SRDLILEAIALFPKQAPVPRLVFMADES---ESGNSSELGKEFFCSYKDLIDRGDPDFKW 180

Query: 202 IRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMFHA 261
           + P SEWDP+++NYTSGTTSSPKGVVH HRGIF +T+DSLI+WGVPK  VYLWTLPMFHA
Sbjct: 181 VMPKSEWDPMILNYTSGTTSSPKGVVHCHRGIFIMTVDSLIDWGVPKQPVYLWTLPMFHA 240

Query: 262 NGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHMCGAPVVLNMLTNSPNNRPLER 321
           NGWS+P+ +AAVGGTNIC+RKFD+ +I+ +I RH VTHMCGAPVVLNML+N+P + PL+ 
Sbjct: 241 NGWSYPWGMAAVGGTNICLRKFDSEIIYDMIKRHGVTHMCGAPVVLNMLSNAPGSEPLKT 300

Query: 322 PVKILTAGAPPPAAVLLRTESLGFDVSHGYGLTETGGLVIFCSWKLEWNQLPATERARLK 381
            V+I+TAGAPPP+AVL RTESLGF VSHGYGLTET GLV+ C+WK EWN LPATERARLK
Sbjct: 301 TVQIMTAGAPPPSAVLFRTESLGFAVSHGYGLTETAGLVVSCAWKKEWNHLPATERARLK 360

Query: 382 ARQGVRTLAMAEADVLDSGTGKRVQRDGVSIGEIVIRGGSMMLGYFKDPEATKKAMTEEG 441
           +RQGV T+   + DV+D  TG  V+RDG ++GE+V+RGGS+MLGY KDPE T K+MT +G
Sbjct: 361 SRQGVGTVMQTKIDVVDPVTGAAVKRDGSTLGEVVLRGGSVMLGYLKDPEGTAKSMTADG 420

Query: 442 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF 501
           WF+TGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVES+LY++P + EAAVVARPDEF
Sbjct: 421 WFYTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESILYSHPDILEAAVVARPDEF 480

Query: 502 WGETPCAFVSLREGLTRVPTEEEIIEYCKGKLAGFMVPKTVVFMEQLPKTSTGKIQKFLL 561
           WGETPCAFVSL++GLT+ PTE+EI+EYC+ KL  +MVPKTVVF E+LPKTSTGK+QKF+L
Sbjct: 481 WGETPCAFVSLKKGLTKKPTEKEIVEYCRSKLPRYMVPKTVVFKEELPKTSTGKVQKFIL 540

Query: 562 REMAKSMG 570
           R+MA+ MG
Sbjct: 541 RDMARGMG 545

BLAST of CsGy5G013370 vs. ExPASy Swiss-Prot
Match: M4ISH2 (Probable CoA ligase CCL11 OS=Humulus lupulus OX=3486 GN=CCL11 PE=2 SV=1)

HSP 1 Score: 760.4 bits (1962), Expect = 1.5e-218
Identity = 369/555 (66.49%), Postives = 451/555 (81.26%), Query Frame = 0

Query: 22  MEHLKPTPANSSPLTPIGFLERASLIYADSPSIIYSSTTFTWSQTYRRCLRLASSLSS-I 81
           ME LKP  ANS PLTP+GFLERA+ +Y D  S++Y   ++TWSQT+ RCLR+AS + S +
Sbjct: 1   MEELKPRFANSCPLTPLGFLERAATVYGDCISVVYDDLSYTWSQTHTRCLRVASCIESCL 60

Query: 82  GIHKGHVVSVIAPNTPPMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQ 141
           G+ KG VVSV+APN P MYEL FAVPMAG +L+ IN RL+A TISV LRHSESKL+FVDQ
Sbjct: 61  GVKKGQVVSVVAPNVPAMYELNFAVPMAGVVLNNINTRLNAVTISVMLRHSESKLVFVDQ 120

Query: 142 ASSGLISDALALFPPEAKRPLLVLISDDAAVEDSASSVAIGGDVVDLYEDMVQKGDPE-F 201
            S  L+ DA++LFP     PLLVLI+D+   E+   +V      V  YED+V+KGD + F
Sbjct: 121 LSVRLVLDAVSLFPKNTPTPLLVLIADNITGEN--LTVEDREHFVCCYEDLVEKGDDKTF 180

Query: 202 RWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMF 261
           +W+RP+SEWDPIV+NYTSGTTSSPKGVVHSHR +F VTLDSLI+WGVPK  VYLWTLPMF
Sbjct: 181 KWVRPISEWDPIVLNYTSGTTSSPKGVVHSHRSVFVVTLDSLIDWGVPKQPVYLWTLPMF 240

Query: 262 HANGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHMCGAPVVLNMLTNSPNNRPL 321
           HANGW + + IAAVGGTNIC+R+FD  +IF+LI RH VTHMC AP+VLNML+NSPN  PL
Sbjct: 241 HANGWGYTWGIAAVGGTNICLRRFDGEIIFNLIRRHRVTHMCAAPIVLNMLSNSPNAEPL 300

Query: 322 ERPVKILTAGAPPPAAVLLRTESLGFDVSHGYGLTETGGLVIFCSWKLEWNQLPATERAR 381
             PV ++T GAPPPAAVLLRTESLGF +SHGYG+TE  G+V+ C+WK EWN+LPATE+AR
Sbjct: 301 PNPVHVMTGGAPPPAAVLLRTESLGFVISHGYGMTEMLGVVVSCAWKREWNRLPATEQAR 360

Query: 382 LKARQGVRTLAMAEADVLDSGTGKRVQRDGVSIGEIVIRGGSMMLGYFKDPEATKKAMTE 441
           LK+RQGVRT AM E DV+D  +G  V+RDG+++GEIV++G S+MLGY K+  AT K +  
Sbjct: 361 LKSRQGVRTAAMMEVDVVDPNSGVSVKRDGLTMGEIVLKGSSIMLGYLKNSAATAKCIRA 420

Query: 442 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 501
           +GWF+TGD+ VMHPDGYLEIKDRSKDVIISGGEN+SSVEVES LY++PAV+EAAVVA PD
Sbjct: 421 DGWFYTGDMAVMHPDGYLEIKDRSKDVIISGGENVSSVEVESALYSHPAVDEAAVVACPD 480

Query: 502 EFWGETPCAFVSLREGLTRVPTEEEIIEYCKGKLAGFMVPKTVVFMEQLPKTSTGKIQKF 561
           E+WGETP AFV+L++G+   PTE+EI+EYC+ KLA FMVPK VVF ++LPKTSTGKIQKF
Sbjct: 481 EYWGETPFAFVTLKKGMRVRPTEKEILEYCREKLAHFMVPKVVVFRDKLPKTSTGKIQKF 540

Query: 562 LLREMAKSMGCSPKS 575
            L+E+ K+MG S  S
Sbjct: 541 TLKEIVKTMGYSSSS 553

BLAST of CsGy5G013370 vs. ExPASy Swiss-Prot
Match: Q9FFE6 (Probable acyl-activating enzyme 5, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE5 PE=1 SV=1)

HSP 1 Score: 745.3 bits (1923), Expect = 5.0e-214
Identity = 365/556 (65.65%), Postives = 451/556 (81.12%), Query Frame = 0

Query: 22  MEHLKPTPANSSPLTPIGFLERASLIYADSPSIIY-SSTTFTWSQTYRRCLRLASSLSSI 81
           ME +KP  ANS PLTPIGFLERA+ +Y D  SI+Y S+T +TW +T  RCLR+ASSLSSI
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 82  GIHKGHVVSVIAPNTPPMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQ 141
           GI +  VVSV++PNTP MYELQFAVPM+GAIL+ IN RLDART+SV LRH  SKLLFVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 142 ASSGLISDALALFPPEAKRPLLVLISDDAAVEDSASSVAIGGDVVDLYEDMVQKGDPEFR 201
            S  L  +A+++   +   P+LV I+D    E   + VA        Y+D++ +GD +F+
Sbjct: 121 FSVDLAVEAISMMTTDP--PILVFIADKEE-EGGDADVADRTKFSYTYDDLIHRGDLDFK 180

Query: 202 WIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMFH 261
           WIRP SEWDP+V+NYTSGTTS+PKGVVH HRGIF +++DSLI+W VPK+ VYLWTLP+FH
Sbjct: 181 WIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPIFH 240

Query: 262 ANGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHMCGAPVVLNMLTNSPNNRPLE 321
           ANGWS+P+ IAAVGGTN+C+RKFDA LI+ LI  H VTHMCGAPVVLNML+ +   +PL 
Sbjct: 241 ANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQPLN 300

Query: 322 RPVKILTAGAPPPAAVLLRTESLGFDVSHGYGLTETGGLVIFCSWKLEWNQLPATERARL 381
           RPV ILTAGAPPPAAVLLR ES+GF +SHGYGLTET GL + C+WK +WN+LPA++RARL
Sbjct: 301 RPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRARL 360

Query: 382 KARQGVRTLAMAEADVLDSGTGKRVQRDGVSIGEIVIRGGSMMLGYFKDPEATKKAMTEE 441
           KARQGVRT+   E DV+D  +G+ V+R+G ++GEIV+RG S+MLGY KDP  T+KA+ + 
Sbjct: 361 KARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKAL-KN 420

Query: 442 GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDE 501
           GWF+TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLYTNPAVNE AVVARPD 
Sbjct: 421 GWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARPDV 480

Query: 502 FWGETPCAFVSLREGLTRVPTEEEIIEYCKGKLAGFMVPKTVVFMEQLPKTSTGKIQKFL 561
           FWGETPCAFVSL+ GLT+ PTE E+IEYC+ K+  +MVPKTV F+++LPKTSTGK+ KF+
Sbjct: 481 FWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMKFV 540

Query: 562 LREMAKSMGCSPKSRL 577
           LRE+AK MG +  SR+
Sbjct: 541 LREIAKKMGTTRLSRM 552

BLAST of CsGy5G013370 vs. ExPASy Swiss-Prot
Match: Q9FFE9 (Probable acyl-activating enzyme 6 OS=Arabidopsis thaliana OX=3702 GN=AAE6 PE=2 SV=1)

HSP 1 Score: 737.3 bits (1902), Expect = 1.4e-211
Identity = 359/555 (64.68%), Postives = 450/555 (81.08%), Query Frame = 0

Query: 22  MEHLKPTPANSSPLTPIGFLERASLIYADSPSIIY-SSTTFTWSQTYRRCLRLASSLSSI 81
           ME +KP  ANS PLTPIGFLERA+ +Y D  SI+Y S+T +TW +T  RCLR+ASSLSSI
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 82  GIHKGHVVSVIAPNTPPMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQ 141
           GI +  VVSV++PNTP MYELQFAVPM+GAIL+ IN RLDART+SV LRH ESKLLFVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 142 ASSGLISDALALFPPEAKRPLLVLISDDAAVEDSASSVAIGGDVVDL------YEDMVQK 201
            S  L  +A+++   +   P+LV+I+D    ++    VA   DV DL      Y+D++++
Sbjct: 121 FSVDLAVEAVSMMTTDP--PILVVIAD----KEEEGGVA---DVADLSKFSYTYDDLIER 180

Query: 202 GDPEFRWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLW 261
           GDP F+WIRP SEWDP+V+NYTSGTTS+PKGVVH HRGIF +++DSLI+W VPK+ VYLW
Sbjct: 181 GDPGFKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLW 240

Query: 262 TLPMFHANGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHMCGAPVVLNMLTNSP 321
           TLP+FH+NGW+ P+ IAAVGGTN+C+RKFDA LI+ LI  H VTHMCGAPVVLNML+ + 
Sbjct: 241 TLPIFHSNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQ 300

Query: 322 NNRPLERPVKILTAGAPPPAAVLLRTESLGFDVSHGYGLTETGGLVIFCSWKLEWNQLPA 381
            ++PL  PV ILTAG+PPPA VLLR ES+GF +SHGYGLTET G+++ C+WK +WN LPA
Sbjct: 301 ESQPLNHPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPA 360

Query: 382 TERARLKARQGVRTLAMAEADVLDSGTGKRVQRDGVSIGEIVIRGGSMMLGYFKDPEATK 441
           ++RARLKARQGVRT+   E DV+D  +G  V+R+G ++GEIV+RG S+MLGY KDP  T+
Sbjct: 361 SDRARLKARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTE 420

Query: 442 KAMTEEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAV 501
           KA+ + GWF+TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLYT PAVNE AV
Sbjct: 421 KAL-KNGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAV 480

Query: 502 VARPDEFWGETPCAFVSLREGLTRVPTEEEIIEYCKGKLAGFMVPKTVVFMEQLPKTSTG 561
           VARPDEFWGETPCAFVSL+ G +  PTEEE++EYC+ K+  +MVPKTV FM++LPK+STG
Sbjct: 481 VARPDEFWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTG 540

Query: 562 KIQKFLLREMAKSMG 570
           K+ KF+LR++AK MG
Sbjct: 541 KVTKFVLRDIAKKMG 545

BLAST of CsGy5G013370 vs. ExPASy Swiss-Prot
Match: O80658 (Probable acyl-activating enzyme 4 OS=Arabidopsis thaliana OX=3702 GN=AEE4 PE=2 SV=1)

HSP 1 Score: 709.1 bits (1829), Expect = 4.0e-203
Identity = 340/549 (61.93%), Postives = 437/549 (79.60%), Query Frame = 0

Query: 22  MEHLKPTPANSSPLTPIGFLERASLIYADSPSIIYSSTTFTWSQTYRRCLRLASSLS--S 81
           ME L P  +NS PLT +GFLERA+ ++ DSPS+++++T  TWS+T+ RCLR+AS+LS  S
Sbjct: 1   MELLLPHASNSCPLTVLGFLERAASVFGDSPSLLHTTTVHTWSETHSRCLRIASTLSSAS 60

Query: 82  IGIHKGHVVSVIAPNTPPMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFVD 141
           +GI++G VVSVI PN P +YELQFAVPM+GA+L+ IN RLDA  +SV LRHSESKL+FVD
Sbjct: 61  LGINRGQVVSVIGPNVPSVYELQFAVPMSGAVLNNINPRLDAHALSVLLRHSESKLVFVD 120

Query: 142 QASSGLISDALALFPPEAKRPLLVLISDDAAVEDSASSVAIGGDVVDLYEDMVQKGDPEF 201
             SS L+ +A++ F P+ +RP LV+++D     D  SS +   D +D YE  +++GD  F
Sbjct: 121 HHSSSLVLEAVS-FLPKDERPRLVILNDG---NDMPSSSSADMDFLDTYEGFMERGDLRF 180

Query: 202 RWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMF 261
           +W+RP SEW P+V+NYTSGTTSSPKGVVHSHR +F  T++SL++W +P   VYLWTLPMF
Sbjct: 181 KWVRPKSEWTPMVLNYTSGTTSSPKGVVHSHRSVFMSTINSLLDWSLPNRPVYLWTLPMF 240

Query: 262 HANGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHMCGAPVVLNMLTNSPNNRPL 321
           HANGWS+ +A AAVG  NIC+ + D   IF+LI+++ VTHMC AP+VLNMLTN P  +PL
Sbjct: 241 HANGWSYTWATAAVGARNICVTRVDVPTIFNLIDKYQVTHMCAAPMVLNMLTNHPAQKPL 300

Query: 322 ERPVKILTAGAPPPAAVLLRTESLGFDVSHGYGLTETGGLVIFCSWKLEWNQLPATERAR 381
           + PVK++TAGAPPPA V+ + E+LGFDVSHGYG+TETGGLV+ C+ K EW++L   ERA+
Sbjct: 301 QSPVKVMTAGAPPPATVISKAEALGFDVSHGYGMTETGGLVVSCALKPEWDRLEPDERAK 360

Query: 382 LKARQGVRTLAMAEADVLDSGTGKRVQRDGVSIGEIVIRGGSMMLGYFKDPEATKKAMTE 441
            K+RQG+RT   AE DV D  +GK V+ DG ++GEIV RGGS+MLGY+KDPE T  +M E
Sbjct: 361 QKSRQGIRTAVFAEVDVRDPISGKSVKHDGATVGEIVFRGGSVMLGYYKDPEGTAASMRE 420

Query: 442 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 501
           +GWF+TGD+GVMHPDGYLE+KDRSKDV+I GGEN+SS E+E+VLYTNPA+ EAAVVA+PD
Sbjct: 421 DGWFYTGDIGVMHPDGYLEVKDRSKDVVICGGENISSTELEAVLYTNPAIKEAAVVAKPD 480

Query: 502 EFWGETPCAFVSLREGLTRVPTEEEIIEYCKGKLAGFMVPKTVVFMEQLPKTSTGKIQKF 561
           + WGETPCAFVSL+     V TE EI E+CK KL  +MVP+ VVF+E+LPKTSTGKIQKF
Sbjct: 481 KMWGETPCAFVSLKYHDGSV-TEREIREFCKTKLPKYMVPRNVVFLEELPKTSTGKIQKF 540

Query: 562 LLREMAKSM 569
           LLR+MAKS+
Sbjct: 541 LLRQMAKSL 544

BLAST of CsGy5G013370 vs. NCBI nr
Match: XP_004148909.1 (probable acyl-activating enzyme 5, peroxisomal [Cucumis sativus] >KGN50896.1 hypothetical protein Csa_023073 [Cucumis sativus])

HSP 1 Score: 1156 bits (2990), Expect = 0.0
Identity = 576/576 (100.00%), Postives = 576/576 (100.00%), Query Frame = 0

Query: 1   MYRGEKQINNHTTTSLSLPISMEHLKPTPANSSPLTPIGFLERASLIYADSPSIIYSSTT 60
           MYRGEKQINNHTTTSLSLPISMEHLKPTPANSSPLTPIGFLERASLIYADSPSIIYSSTT
Sbjct: 1   MYRGEKQINNHTTTSLSLPISMEHLKPTPANSSPLTPIGFLERASLIYADSPSIIYSSTT 60

Query: 61  FTWSQTYRRCLRLASSLSSIGIHKGHVVSVIAPNTPPMYELQFAVPMAGAILSCINLRLD 120
           FTWSQTYRRCLRLASSLSSIGIHKGHVVSVIAPNTPPMYELQFAVPMAGAILSCINLRLD
Sbjct: 61  FTWSQTYRRCLRLASSLSSIGIHKGHVVSVIAPNTPPMYELQFAVPMAGAILSCINLRLD 120

Query: 121 ARTISVQLRHSESKLLFVDQASSGLISDALALFPPEAKRPLLVLISDDAAVEDSASSVAI 180
           ARTISVQLRHSESKLLFVDQASSGLISDALALFPPEAKRPLLVLISDDAAVEDSASSVAI
Sbjct: 121 ARTISVQLRHSESKLLFVDQASSGLISDALALFPPEAKRPLLVLISDDAAVEDSASSVAI 180

Query: 181 GGDVVDLYEDMVQKGDPEFRWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDS 240
           GGDVVDLYEDMVQKGDPEFRWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDS
Sbjct: 181 GGDVVDLYEDMVQKGDPEFRWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDS 240

Query: 241 LIEWGVPKHSVYLWTLPMFHANGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHM 300
           LIEWGVPKHSVYLWTLPMFHANGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHM
Sbjct: 241 LIEWGVPKHSVYLWTLPMFHANGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHM 300

Query: 301 CGAPVVLNMLTNSPNNRPLERPVKILTAGAPPPAAVLLRTESLGFDVSHGYGLTETGGLV 360
           CGAPVVLNMLTNSPNNRPLERPVKILTAGAPPPAAVLLRTESLGFDVSHGYGLTETGGLV
Sbjct: 301 CGAPVVLNMLTNSPNNRPLERPVKILTAGAPPPAAVLLRTESLGFDVSHGYGLTETGGLV 360

Query: 361 IFCSWKLEWNQLPATERARLKARQGVRTLAMAEADVLDSGTGKRVQRDGVSIGEIVIRGG 420
           IFCSWKLEWNQLPATERARLKARQGVRTLAMAEADVLDSGTGKRVQRDGVSIGEIVIRGG
Sbjct: 361 IFCSWKLEWNQLPATERARLKARQGVRTLAMAEADVLDSGTGKRVQRDGVSIGEIVIRGG 420

Query: 421 SMMLGYFKDPEATKKAMTEEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVE 480
           SMMLGYFKDPEATKKAMTEEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVE
Sbjct: 421 SMMLGYFKDPEATKKAMTEEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVE 480

Query: 481 SVLYTNPAVNEAAVVARPDEFWGETPCAFVSLREGLTRVPTEEEIIEYCKGKLAGFMVPK 540
           SVLYTNPAVNEAAVVARPDEFWGETPCAFVSLREGLTRVPTEEEIIEYCKGKLAGFMVPK
Sbjct: 481 SVLYTNPAVNEAAVVARPDEFWGETPCAFVSLREGLTRVPTEEEIIEYCKGKLAGFMVPK 540

Query: 541 TVVFMEQLPKTSTGKIQKFLLREMAKSMGCSPKSRL 576
           TVVFMEQLPKTSTGKIQKFLLREMAKSMGCSPKSRL
Sbjct: 541 TVVFMEQLPKTSTGKIQKFLLREMAKSMGCSPKSRL 576

BLAST of CsGy5G013370 vs. NCBI nr
Match: XP_008463175.1 (PREDICTED: probable acyl-activating enzyme 5, peroxisomal [Cucumis melo] >KAA0046215.1 putative acyl-activating enzyme 5, peroxisomal [Cucumis melo var. makuwa] >TYK18981.1 putative acyl-activating enzyme 5, peroxisomal [Cucumis melo var. makuwa])

HSP 1 Score: 1069 bits (2765), Expect = 0.0
Identity = 528/555 (95.14%), Postives = 544/555 (98.02%), Query Frame = 0

Query: 22  MEHLKPTPANSSPLTPIGFLERASLIYADSPSIIYSSTTFTWSQTYRRCLRLASSLSSIG 81
           M+HLKPTPANSSPLTPI FLERASL+YADSPSIIY+ST+FTWSQTY RCLRLASSLSS+G
Sbjct: 1   MDHLKPTPANSSPLTPISFLERASLVYADSPSIIYNSTSFTWSQTYLRCLRLASSLSSLG 60

Query: 82  IHKGHVVSVIAPNTPPMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 141
           IHKGHVVSVI+PNTPPMYELQF+VPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA
Sbjct: 61  IHKGHVVSVISPNTPPMYELQFSVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 120

Query: 142 SSGLISDALALFPPEAKRPLLVLISDDAAVEDSASSVAIGGDVVDLYEDMVQKGDPEFRW 201
           SS LISDAL LFPPE+KRPLLVLI+DDAAVEDSASSVAIGGD VD YEDM+QKGDPEFRW
Sbjct: 121 SSALISDALTLFPPESKRPLLVLIADDAAVEDSASSVAIGGDFVDFYEDMIQKGDPEFRW 180

Query: 202 IRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMFHA 261
           IRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMFHA
Sbjct: 181 IRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMFHA 240

Query: 262 NGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHMCGAPVVLNMLTNSPNNRPLER 321
           NGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTH+CGAPVVLNMLTNSPNNRPLER
Sbjct: 241 NGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHLCGAPVVLNMLTNSPNNRPLER 300

Query: 322 PVKILTAGAPPPAAVLLRTESLGFDVSHGYGLTETGGLVIFCSWKLEWNQLPATERARLK 381
           PVKILTAGAPPPAAVL RTESLGFDVSHGYGLTETGGLVIFCSWKL+WNQLPATERARLK
Sbjct: 301 PVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQLPATERARLK 360

Query: 382 ARQGVRTLAMAEADVLDSGTGKRVQRDGVSIGEIVIRGGSMMLGYFKDPEATKKAMTEEG 441
           ARQGVRTLAMAEADVLDS TGK V+RDGVSIGEIV+RGGSMMLGYFKDPEATKKAMTEEG
Sbjct: 361 ARQGVRTLAMAEADVLDSETGKSVKRDGVSIGEIVLRGGSMMLGYFKDPEATKKAMTEEG 420

Query: 442 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF 501
           WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF
Sbjct: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF 480

Query: 502 WGETPCAFVSLREGLTRVPTEEEIIEYCKGKLAGFMVPKTVVFMEQLPKTSTGKIQKFLL 561
           WGETPCAFVSLREGL+RVPTEEEIIEYC+GKLAGFMVPKTVVFMEQLPKTSTGKIQKFLL
Sbjct: 481 WGETPCAFVSLREGLSRVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTSTGKIQKFLL 540

Query: 562 REMAKSMGCSPKSRL 576
           REMAKSMG S KSRL
Sbjct: 541 REMAKSMGPSRKSRL 555

BLAST of CsGy5G013370 vs. NCBI nr
Match: XP_038877068.1 (probable acyl-activating enzyme 5, peroxisomal [Benincasa hispida])

HSP 1 Score: 1009 bits (2609), Expect = 0.0
Identity = 499/577 (86.48%), Postives = 537/577 (93.07%), Query Frame = 0

Query: 3   RGEKQINNHTTTSLSLPISMEHLKPTPANSSPLTPIGFLERASLIYADSPSIIYSSTTFT 62
           + EKQ N++  T++S  ISME LKP+ ANSSPLTPIGFLERAS+IY DSPSIIY+STT+T
Sbjct: 9   KREKQSNHNRATTISQSISMEDLKPSLANSSPLTPIGFLERASVIYGDSPSIIYNSTTYT 68

Query: 63  WSQTYRRCLRLASSLSSIGIHKGHVVSVIAPNTPPMYELQFAVPMAGAILSCINLRLDAR 122
           WS+TYRRCLR+ASSLSSIGI KG+VVSVIAPNTPPMYELQFAVPMAGA+L+CINLRLDAR
Sbjct: 69  WSETYRRCLRVASSLSSIGIQKGYVVSVIAPNTPPMYELQFAVPMAGAVLNCINLRLDAR 128

Query: 123 TISVQLRHSESKLLFVDQASSGLISDALALFPPEAKRPLLVLISDDAAVEDS---ASSVA 182
           TIS+ LRH ESKL+F+DQAS GLISDALALFPPE KRPLLVLI+DD AVEDS   +SS  
Sbjct: 129 TISILLRHCESKLIFIDQASCGLISDALALFPPETKRPLLVLIADDTAVEDSLSSSSSSV 188

Query: 183 IGGDVVDLYEDMVQKGDPEFRWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLD 242
           IGG+ VD YE+MVQKGD EFRWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIF VTLD
Sbjct: 189 IGGEFVDSYEEMVQKGDDEFRWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFAVTLD 248

Query: 243 SLIEWGVPKHSVYLWTLPMFHANGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTH 302
           SLIEWGVPK SVYLWTLPMFHANGWSFPF IAAVGGTN+CIRKFDAALIFSLI+RHHVTH
Sbjct: 249 SLIEWGVPKQSVYLWTLPMFHANGWSFPFGIAAVGGTNVCIRKFDAALIFSLIHRHHVTH 308

Query: 303 MCGAPVVLNMLTNSPNNRPLERPVKILTAGAPPPAAVLLRTESLGFDVSHGYGLTETGGL 362
           +CGAPVVLNMLTNSP+N+PLERPVKILTAGAPPPAAVL RTESLGFDV HGYGLTETGGL
Sbjct: 309 LCGAPVVLNMLTNSPDNQPLERPVKILTAGAPPPAAVLFRTESLGFDVGHGYGLTETGGL 368

Query: 363 VIFCSWKLEWNQLPATERARLKARQGVRTLAMAEADVLDSGTGKRVQRDGVSIGEIVIRG 422
           VI+CSWK +WN+LPATERARLKARQGVRTLAM+E DVLDS TGK V+RDG SIGEIV+RG
Sbjct: 369 VIYCSWKSKWNRLPATERARLKARQGVRTLAMSEVDVLDSETGKSVKRDGASIGEIVLRG 428

Query: 423 GSMMLGYFKDPEATKKAMTEEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEV 482
           GSMMLGYFKDPEATKKAMT+EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEV
Sbjct: 429 GSMMLGYFKDPEATKKAMTDEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEV 488

Query: 483 ESVLYTNPAVNEAAVVARPDEFWGETPCAFVSLREGLTRVPTEEEIIEYCKGKLAGFMVP 542
           ESVLYTNPAVNEAAVVARPDEFWGETPCAFVSLREGLTR+PTEEEI+EYC+ KL  FMVP
Sbjct: 489 ESVLYTNPAVNEAAVVARPDEFWGETPCAFVSLREGLTRLPTEEEIMEYCREKLPKFMVP 548

Query: 543 KTVVFMEQLPKTSTGKIQKFLLREMAKSMGCSPKSRL 576
           KTVVFME+LPKTSTGKIQKFLLRE AKSMG +  SRL
Sbjct: 549 KTVVFMEKLPKTSTGKIQKFLLREKAKSMGPARTSRL 585

BLAST of CsGy5G013370 vs. NCBI nr
Match: XP_022939278.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita moschata] >KAG6579057.1 putative acyl-activating enzyme 5, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 935 bits (2417), Expect = 0.0
Identity = 465/557 (83.48%), Postives = 503/557 (90.31%), Query Frame = 0

Query: 22  MEHLKPTPANSSPLTPIGFLERASLIYADSPSIIYSSTTFTWSQTYRRCLRLASSLSSIG 81
           ME LKP PANSSPLTPIGFLERA+++Y D PSIIY+STT+TW++T+RRCL+LASSL SIG
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 82  IHKGHVVSVIAPNTPPMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 141
           I +G VVSV++PNT  MYEL FAVPMAGA+L+ INLRLDARTISV LRH E+ L+FVDQA
Sbjct: 61  IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120

Query: 142 SSGLISDALALFPPEAKRPLLVLISDDAAVEDS--ASSVAIGGDVVDLYEDMVQKGDPEF 201
           S GLISDALALFPPE KRP L+LI+D+AA EDS  ASSV    D VD YE+MVQKGDP F
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSASSVWNRADFVDSYEEMVQKGDPLF 180

Query: 202 RWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMF 261
           +WIRP SEWDPIV+NYTSGTTSSPKGVVH HRGIFTVTLDSLIEWGVPK SVYLWTLPMF
Sbjct: 181 QWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWTLPMF 240

Query: 262 HANGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHMCGAPVVLNMLTNSPNNRPL 321
           HANGWSFP+ IAAVGGTN+CIRKFDAA IF+LI+R+HVTH+CGAPVVLNMLTNSP+NRPL
Sbjct: 241 HANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPDNRPL 300

Query: 322 ERPVKILTAGAPPPAAVLLRTESLGFDVSHGYGLTETGGLVIFCSWKLEWNQLPATERAR 381
           +RPVKILTAGAPPPAAVL RTESLGFDVSHGYGLTETGGLVI CSWK  WN+LPA ERAR
Sbjct: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAAERAR 360

Query: 382 LKARQGVRTLAMAEADVLDSGTGKRVQRDGVSIGEIVIRGGSMMLGYFKDPEATKKAMTE 441
           LKARQGVRTLAMA ADVLD  TGK V+RDG SIGEIV+RG SMMLGY KDPEAT KAMT 
Sbjct: 361 LKARQGVRTLAMAVADVLDPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAKAMTA 420

Query: 442 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 501
           EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD
Sbjct: 421 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480

Query: 502 EFWGETPCAFVSLREGLTRVPTEEEIIEYCKGKLAGFMVPKTVVFMEQLPKTSTGKIQKF 561
           EFWGETPCAFVSL+EGLTR PTE EIIEYC+GKL  FMVPKTVVF  +LPKTSTGKIQKF
Sbjct: 481 EFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540

Query: 562 LLREMAKSMGCSPKSRL 576
           +LREMAKSMG + KSRL
Sbjct: 541 VLREMAKSMGPAGKSRL 557

BLAST of CsGy5G013370 vs. NCBI nr
Match: XP_022993747.1 (probable acyl-activating enzyme 5, peroxisomal [Cucurbita maxima])

HSP 1 Score: 933 bits (2412), Expect = 0.0
Identity = 464/561 (82.71%), Postives = 505/561 (90.02%), Query Frame = 0

Query: 22  MEHLKPTPANSSPLTPIGFLERASLIYADSPSIIYSSTTFTWSQTYRRCLRLASSLSSIG 81
           ME LKP PANSSPLTPIGFLERA+++Y D PSIIY+STT+TW++T+RRCL+LASSL SIG
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 82  IHKGHVVSVIAPNTPPMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 141
           I +G VVSV++PNTP MYEL FAVPMAGA+L+ INLRLD+RTISV LRH E+ L+FVDQA
Sbjct: 61  IQRGQVVSVVSPNTPAMYELHFAVPMAGAVLNTINLRLDSRTISVILRHCETNLIFVDQA 120

Query: 142 SSGLISDALALFPPEAKRPLLVLISDDAAVEDS------ASSVAIGGDVVDLYEDMVQKG 201
           S GLISDALALFPPE KRP L+LI+D+AA EDS      ASSV+   D VD YE+MVQKG
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSSSSSASSVSNRADFVDSYEEMVQKG 180

Query: 202 DPEFRWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWT 261
           DP F+WIRPVSEWDPIV+NYTSGTTSSPKGVVH HRGIFTVTLDSLIEWGV K SVYLWT
Sbjct: 181 DPLFQWIRPVSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVSKQSVYLWT 240

Query: 262 LPMFHANGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHMCGAPVVLNMLTNSPN 321
           LPMFHANGWSFP+ IAAVGGTN+CIRKFDAA IF+LI+R+HVTH+CGAPVVLNMLTNSP+
Sbjct: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDAATIFALIHRYHVTHLCGAPVVLNMLTNSPD 300

Query: 322 NRPLERPVKILTAGAPPPAAVLLRTESLGFDVSHGYGLTETGGLVIFCSWKLEWNQLPAT 381
           NRPL+RPVKILTAGAPPPAAVL RTESLGFDVSHGYGLTETGGLVI CSWK  WN+LPA 
Sbjct: 301 NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAA 360

Query: 382 ERARLKARQGVRTLAMAEADVLDSGTGKRVQRDGVSIGEIVIRGGSMMLGYFKDPEATKK 441
           ERARLKARQGVRTLAMA ADVL   TGK V+RDG SIGEIV+RG SMMLGY KDPEAT K
Sbjct: 361 ERARLKARQGVRTLAMAVADVLCPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAK 420

Query: 442 AMTEEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 501
           AMT EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV
Sbjct: 421 AMTAEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480

Query: 502 ARPDEFWGETPCAFVSLREGLTRVPTEEEIIEYCKGKLAGFMVPKTVVFMEQLPKTSTGK 561
           ARPDEFWGETPCAFVSL+EGLTR PTE EIIEYC+GKL  FMVPKTVVFM +LPKTSTGK
Sbjct: 481 ARPDEFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFMAELPKTSTGK 540

Query: 562 IQKFLLREMAKSMGCSPKSRL 576
           IQKF+LREMAKSMG + +SRL
Sbjct: 541 IQKFVLREMAKSMGPAGRSRL 561

BLAST of CsGy5G013370 vs. ExPASy TrEMBL
Match: A0A0A0KT33 (AMP dependent ligase OS=Cucumis sativus OX=3659 GN=Csa_5G317890 PE=4 SV=1)

HSP 1 Score: 1156 bits (2990), Expect = 0.0
Identity = 576/576 (100.00%), Postives = 576/576 (100.00%), Query Frame = 0

Query: 1   MYRGEKQINNHTTTSLSLPISMEHLKPTPANSSPLTPIGFLERASLIYADSPSIIYSSTT 60
           MYRGEKQINNHTTTSLSLPISMEHLKPTPANSSPLTPIGFLERASLIYADSPSIIYSSTT
Sbjct: 1   MYRGEKQINNHTTTSLSLPISMEHLKPTPANSSPLTPIGFLERASLIYADSPSIIYSSTT 60

Query: 61  FTWSQTYRRCLRLASSLSSIGIHKGHVVSVIAPNTPPMYELQFAVPMAGAILSCINLRLD 120
           FTWSQTYRRCLRLASSLSSIGIHKGHVVSVIAPNTPPMYELQFAVPMAGAILSCINLRLD
Sbjct: 61  FTWSQTYRRCLRLASSLSSIGIHKGHVVSVIAPNTPPMYELQFAVPMAGAILSCINLRLD 120

Query: 121 ARTISVQLRHSESKLLFVDQASSGLISDALALFPPEAKRPLLVLISDDAAVEDSASSVAI 180
           ARTISVQLRHSESKLLFVDQASSGLISDALALFPPEAKRPLLVLISDDAAVEDSASSVAI
Sbjct: 121 ARTISVQLRHSESKLLFVDQASSGLISDALALFPPEAKRPLLVLISDDAAVEDSASSVAI 180

Query: 181 GGDVVDLYEDMVQKGDPEFRWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDS 240
           GGDVVDLYEDMVQKGDPEFRWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDS
Sbjct: 181 GGDVVDLYEDMVQKGDPEFRWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDS 240

Query: 241 LIEWGVPKHSVYLWTLPMFHANGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHM 300
           LIEWGVPKHSVYLWTLPMFHANGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHM
Sbjct: 241 LIEWGVPKHSVYLWTLPMFHANGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHM 300

Query: 301 CGAPVVLNMLTNSPNNRPLERPVKILTAGAPPPAAVLLRTESLGFDVSHGYGLTETGGLV 360
           CGAPVVLNMLTNSPNNRPLERPVKILTAGAPPPAAVLLRTESLGFDVSHGYGLTETGGLV
Sbjct: 301 CGAPVVLNMLTNSPNNRPLERPVKILTAGAPPPAAVLLRTESLGFDVSHGYGLTETGGLV 360

Query: 361 IFCSWKLEWNQLPATERARLKARQGVRTLAMAEADVLDSGTGKRVQRDGVSIGEIVIRGG 420
           IFCSWKLEWNQLPATERARLKARQGVRTLAMAEADVLDSGTGKRVQRDGVSIGEIVIRGG
Sbjct: 361 IFCSWKLEWNQLPATERARLKARQGVRTLAMAEADVLDSGTGKRVQRDGVSIGEIVIRGG 420

Query: 421 SMMLGYFKDPEATKKAMTEEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVE 480
           SMMLGYFKDPEATKKAMTEEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVE
Sbjct: 421 SMMLGYFKDPEATKKAMTEEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVE 480

Query: 481 SVLYTNPAVNEAAVVARPDEFWGETPCAFVSLREGLTRVPTEEEIIEYCKGKLAGFMVPK 540
           SVLYTNPAVNEAAVVARPDEFWGETPCAFVSLREGLTRVPTEEEIIEYCKGKLAGFMVPK
Sbjct: 481 SVLYTNPAVNEAAVVARPDEFWGETPCAFVSLREGLTRVPTEEEIIEYCKGKLAGFMVPK 540

Query: 541 TVVFMEQLPKTSTGKIQKFLLREMAKSMGCSPKSRL 576
           TVVFMEQLPKTSTGKIQKFLLREMAKSMGCSPKSRL
Sbjct: 541 TVVFMEQLPKTSTGKIQKFLLREMAKSMGCSPKSRL 576

BLAST of CsGy5G013370 vs. ExPASy TrEMBL
Match: A0A5A7TXV0 (Putative acyl-activating enzyme 5, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold418G00770 PE=4 SV=1)

HSP 1 Score: 1069 bits (2765), Expect = 0.0
Identity = 528/555 (95.14%), Postives = 544/555 (98.02%), Query Frame = 0

Query: 22  MEHLKPTPANSSPLTPIGFLERASLIYADSPSIIYSSTTFTWSQTYRRCLRLASSLSSIG 81
           M+HLKPTPANSSPLTPI FLERASL+YADSPSIIY+ST+FTWSQTY RCLRLASSLSS+G
Sbjct: 1   MDHLKPTPANSSPLTPISFLERASLVYADSPSIIYNSTSFTWSQTYLRCLRLASSLSSLG 60

Query: 82  IHKGHVVSVIAPNTPPMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 141
           IHKGHVVSVI+PNTPPMYELQF+VPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA
Sbjct: 61  IHKGHVVSVISPNTPPMYELQFSVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 120

Query: 142 SSGLISDALALFPPEAKRPLLVLISDDAAVEDSASSVAIGGDVVDLYEDMVQKGDPEFRW 201
           SS LISDAL LFPPE+KRPLLVLI+DDAAVEDSASSVAIGGD VD YEDM+QKGDPEFRW
Sbjct: 121 SSALISDALTLFPPESKRPLLVLIADDAAVEDSASSVAIGGDFVDFYEDMIQKGDPEFRW 180

Query: 202 IRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMFHA 261
           IRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMFHA
Sbjct: 181 IRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMFHA 240

Query: 262 NGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHMCGAPVVLNMLTNSPNNRPLER 321
           NGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTH+CGAPVVLNMLTNSPNNRPLER
Sbjct: 241 NGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHLCGAPVVLNMLTNSPNNRPLER 300

Query: 322 PVKILTAGAPPPAAVLLRTESLGFDVSHGYGLTETGGLVIFCSWKLEWNQLPATERARLK 381
           PVKILTAGAPPPAAVL RTESLGFDVSHGYGLTETGGLVIFCSWKL+WNQLPATERARLK
Sbjct: 301 PVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQLPATERARLK 360

Query: 382 ARQGVRTLAMAEADVLDSGTGKRVQRDGVSIGEIVIRGGSMMLGYFKDPEATKKAMTEEG 441
           ARQGVRTLAMAEADVLDS TGK V+RDGVSIGEIV+RGGSMMLGYFKDPEATKKAMTEEG
Sbjct: 361 ARQGVRTLAMAEADVLDSETGKSVKRDGVSIGEIVLRGGSMMLGYFKDPEATKKAMTEEG 420

Query: 442 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF 501
           WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF
Sbjct: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF 480

Query: 502 WGETPCAFVSLREGLTRVPTEEEIIEYCKGKLAGFMVPKTVVFMEQLPKTSTGKIQKFLL 561
           WGETPCAFVSLREGL+RVPTEEEIIEYC+GKLAGFMVPKTVVFMEQLPKTSTGKIQKFLL
Sbjct: 481 WGETPCAFVSLREGLSRVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTSTGKIQKFLL 540

Query: 562 REMAKSMGCSPKSRL 576
           REMAKSMG S KSRL
Sbjct: 541 REMAKSMGPSRKSRL 555

BLAST of CsGy5G013370 vs. ExPASy TrEMBL
Match: A0A1S3CIM6 (probable acyl-activating enzyme 5, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103501383 PE=4 SV=1)

HSP 1 Score: 1069 bits (2765), Expect = 0.0
Identity = 528/555 (95.14%), Postives = 544/555 (98.02%), Query Frame = 0

Query: 22  MEHLKPTPANSSPLTPIGFLERASLIYADSPSIIYSSTTFTWSQTYRRCLRLASSLSSIG 81
           M+HLKPTPANSSPLTPI FLERASL+YADSPSIIY+ST+FTWSQTY RCLRLASSLSS+G
Sbjct: 1   MDHLKPTPANSSPLTPISFLERASLVYADSPSIIYNSTSFTWSQTYLRCLRLASSLSSLG 60

Query: 82  IHKGHVVSVIAPNTPPMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 141
           IHKGHVVSVI+PNTPPMYELQF+VPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA
Sbjct: 61  IHKGHVVSVISPNTPPMYELQFSVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 120

Query: 142 SSGLISDALALFPPEAKRPLLVLISDDAAVEDSASSVAIGGDVVDLYEDMVQKGDPEFRW 201
           SS LISDAL LFPPE+KRPLLVLI+DDAAVEDSASSVAIGGD VD YEDM+QKGDPEFRW
Sbjct: 121 SSALISDALTLFPPESKRPLLVLIADDAAVEDSASSVAIGGDFVDFYEDMIQKGDPEFRW 180

Query: 202 IRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMFHA 261
           IRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMFHA
Sbjct: 181 IRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMFHA 240

Query: 262 NGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHMCGAPVVLNMLTNSPNNRPLER 321
           NGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTH+CGAPVVLNMLTNSPNNRPLER
Sbjct: 241 NGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHLCGAPVVLNMLTNSPNNRPLER 300

Query: 322 PVKILTAGAPPPAAVLLRTESLGFDVSHGYGLTETGGLVIFCSWKLEWNQLPATERARLK 381
           PVKILTAGAPPPAAVL RTESLGFDVSHGYGLTETGGLVIFCSWKL+WNQLPATERARLK
Sbjct: 301 PVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVIFCSWKLKWNQLPATERARLK 360

Query: 382 ARQGVRTLAMAEADVLDSGTGKRVQRDGVSIGEIVIRGGSMMLGYFKDPEATKKAMTEEG 441
           ARQGVRTLAMAEADVLDS TGK V+RDGVSIGEIV+RGGSMMLGYFKDPEATKKAMTEEG
Sbjct: 361 ARQGVRTLAMAEADVLDSETGKSVKRDGVSIGEIVLRGGSMMLGYFKDPEATKKAMTEEG 420

Query: 442 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF 501
           WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF
Sbjct: 421 WFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDEF 480

Query: 502 WGETPCAFVSLREGLTRVPTEEEIIEYCKGKLAGFMVPKTVVFMEQLPKTSTGKIQKFLL 561
           WGETPCAFVSLREGL+RVPTEEEIIEYC+GKLAGFMVPKTVVFMEQLPKTSTGKIQKFLL
Sbjct: 481 WGETPCAFVSLREGLSRVPTEEEIIEYCRGKLAGFMVPKTVVFMEQLPKTSTGKIQKFLL 540

Query: 562 REMAKSMGCSPKSRL 576
           REMAKSMG S KSRL
Sbjct: 541 REMAKSMGPSRKSRL 555

BLAST of CsGy5G013370 vs. ExPASy TrEMBL
Match: A0A6J1FL76 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111445243 PE=4 SV=1)

HSP 1 Score: 935 bits (2417), Expect = 0.0
Identity = 465/557 (83.48%), Postives = 503/557 (90.31%), Query Frame = 0

Query: 22  MEHLKPTPANSSPLTPIGFLERASLIYADSPSIIYSSTTFTWSQTYRRCLRLASSLSSIG 81
           ME LKP PANSSPLTPIGFLERA+++Y D PSIIY+STT+TW++T+RRCL+LASSL SIG
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 82  IHKGHVVSVIAPNTPPMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 141
           I +G VVSV++PNT  MYEL FAVPMAGA+L+ INLRLDARTISV LRH E+ L+FVDQA
Sbjct: 61  IQRGQVVSVVSPNTAAMYELHFAVPMAGAVLNTINLRLDARTISVILRHCETNLIFVDQA 120

Query: 142 SSGLISDALALFPPEAKRPLLVLISDDAAVEDS--ASSVAIGGDVVDLYEDMVQKGDPEF 201
           S GLISDALALFPPE KRP L+LI+D+AA EDS  ASSV    D VD YE+MVQKGDP F
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSASSVWNRADFVDSYEEMVQKGDPLF 180

Query: 202 RWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMF 261
           +WIRP SEWDPIV+NYTSGTTSSPKGVVH HRGIFTVTLDSLIEWGVPK SVYLWTLPMF
Sbjct: 181 QWIRPASEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVPKESVYLWTLPMF 240

Query: 262 HANGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHMCGAPVVLNMLTNSPNNRPL 321
           HANGWSFP+ IAAVGGTN+CIRKFDAA IF+LI+R+HVTH+CGAPVVLNMLTNSP+NRPL
Sbjct: 241 HANGWSFPWGIAAVGGTNVCIRKFDAAAIFALIHRYHVTHLCGAPVVLNMLTNSPDNRPL 300

Query: 322 ERPVKILTAGAPPPAAVLLRTESLGFDVSHGYGLTETGGLVIFCSWKLEWNQLPATERAR 381
           +RPVKILTAGAPPPAAVL RTESLGFDVSHGYGLTETGGLVI CSWK  WN+LPA ERAR
Sbjct: 301 DRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAAERAR 360

Query: 382 LKARQGVRTLAMAEADVLDSGTGKRVQRDGVSIGEIVIRGGSMMLGYFKDPEATKKAMTE 441
           LKARQGVRTLAMA ADVLD  TGK V+RDG SIGEIV+RG SMMLGY KDPEAT KAMT 
Sbjct: 361 LKARQGVRTLAMAVADVLDPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAKAMTA 420

Query: 442 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 501
           EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD
Sbjct: 421 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 480

Query: 502 EFWGETPCAFVSLREGLTRVPTEEEIIEYCKGKLAGFMVPKTVVFMEQLPKTSTGKIQKF 561
           EFWGETPCAFVSL+EGLTR PTE EIIEYC+GKL  FMVPKTVVF  +LPKTSTGKIQKF
Sbjct: 481 EFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFTAELPKTSTGKIQKF 540

Query: 562 LLREMAKSMGCSPKSRL 576
           +LREMAKSMG + KSRL
Sbjct: 541 VLREMAKSMGPAGKSRL 557

BLAST of CsGy5G013370 vs. ExPASy TrEMBL
Match: A0A6J1JTQ2 (probable acyl-activating enzyme 5, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111489657 PE=4 SV=1)

HSP 1 Score: 933 bits (2412), Expect = 0.0
Identity = 464/561 (82.71%), Postives = 505/561 (90.02%), Query Frame = 0

Query: 22  MEHLKPTPANSSPLTPIGFLERASLIYADSPSIIYSSTTFTWSQTYRRCLRLASSLSSIG 81
           ME LKP PANSSPLTPIGFLERA+++Y D PSIIY+STT+TW++T+RRCL+LASSL SIG
Sbjct: 1   MEDLKPNPANSSPLTPIGFLERAAVVYGDCPSIIYNSTTYTWTETHRRCLQLASSLVSIG 60

Query: 82  IHKGHVVSVIAPNTPPMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQA 141
           I +G VVSV++PNTP MYEL FAVPMAGA+L+ INLRLD+RTISV LRH E+ L+FVDQA
Sbjct: 61  IQRGQVVSVVSPNTPAMYELHFAVPMAGAVLNTINLRLDSRTISVILRHCETNLIFVDQA 120

Query: 142 SSGLISDALALFPPEAKRPLLVLISDDAAVEDS------ASSVAIGGDVVDLYEDMVQKG 201
           S GLISDALALFPPE KRP L+LI+D+AA EDS      ASSV+   D VD YE+MVQKG
Sbjct: 121 SCGLISDALALFPPEMKRPPLILIADNAAAEDSSSSSSSASSVSNRADFVDSYEEMVQKG 180

Query: 202 DPEFRWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWT 261
           DP F+WIRPVSEWDPIV+NYTSGTTSSPKGVVH HRGIFTVTLDSLIEWGV K SVYLWT
Sbjct: 181 DPLFQWIRPVSEWDPIVLNYTSGTTSSPKGVVHCHRGIFTVTLDSLIEWGVSKQSVYLWT 240

Query: 262 LPMFHANGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHMCGAPVVLNMLTNSPN 321
           LPMFHANGWSFP+ IAAVGGTN+CIRKFDAA IF+LI+R+HVTH+CGAPVVLNMLTNSP+
Sbjct: 241 LPMFHANGWSFPWGIAAVGGTNVCIRKFDAATIFALIHRYHVTHLCGAPVVLNMLTNSPD 300

Query: 322 NRPLERPVKILTAGAPPPAAVLLRTESLGFDVSHGYGLTETGGLVIFCSWKLEWNQLPAT 381
           NRPL+RPVKILTAGAPPPAAVL RTESLGFDVSHGYGLTETGGLVI CSWK  WN+LPA 
Sbjct: 301 NRPLDRPVKILTAGAPPPAAVLFRTESLGFDVSHGYGLTETGGLVICCSWKSGWNRLPAA 360

Query: 382 ERARLKARQGVRTLAMAEADVLDSGTGKRVQRDGVSIGEIVIRGGSMMLGYFKDPEATKK 441
           ERARLKARQGVRTLAMA ADVL   TGK V+RDG SIGEIV+RG SMMLGY KDPEAT K
Sbjct: 361 ERARLKARQGVRTLAMAVADVLCPETGKSVKRDGTSIGEIVLRGSSMMLGYLKDPEATAK 420

Query: 442 AMTEEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 501
           AMT EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV
Sbjct: 421 AMTAEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVV 480

Query: 502 ARPDEFWGETPCAFVSLREGLTRVPTEEEIIEYCKGKLAGFMVPKTVVFMEQLPKTSTGK 561
           ARPDEFWGETPCAFVSL+EGLTR PTE EIIEYC+GKL  FMVPKTVVFM +LPKTSTGK
Sbjct: 481 ARPDEFWGETPCAFVSLKEGLTRRPTEGEIIEYCRGKLPRFMVPKTVVFMAELPKTSTGK 540

Query: 562 IQKFLLREMAKSMGCSPKSRL 576
           IQKF+LREMAKSMG + +SRL
Sbjct: 541 IQKFVLREMAKSMGPAGRSRL 561

BLAST of CsGy5G013370 vs. TAIR 10
Match: AT5G16370.1 (acyl activating enzyme 5 )

HSP 1 Score: 745.3 bits (1923), Expect = 3.5e-215
Identity = 365/556 (65.65%), Postives = 451/556 (81.12%), Query Frame = 0

Query: 22  MEHLKPTPANSSPLTPIGFLERASLIYADSPSIIY-SSTTFTWSQTYRRCLRLASSLSSI 81
           ME +KP  ANS PLTPIGFLERA+ +Y D  SI+Y S+T +TW +T  RCLR+ASSLSSI
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 82  GIHKGHVVSVIAPNTPPMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQ 141
           GI +  VVSV++PNTP MYELQFAVPM+GAIL+ IN RLDART+SV LRH  SKLLFVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 142 ASSGLISDALALFPPEAKRPLLVLISDDAAVEDSASSVAIGGDVVDLYEDMVQKGDPEFR 201
            S  L  +A+++   +   P+LV I+D    E   + VA        Y+D++ +GD +F+
Sbjct: 121 FSVDLAVEAISMMTTDP--PILVFIADKEE-EGGDADVADRTKFSYTYDDLIHRGDLDFK 180

Query: 202 WIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMFH 261
           WIRP SEWDP+V+NYTSGTTS+PKGVVH HRGIF +++DSLI+W VPK+ VYLWTLP+FH
Sbjct: 181 WIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPIFH 240

Query: 262 ANGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHMCGAPVVLNMLTNSPNNRPLE 321
           ANGWS+P+ IAAVGGTN+C+RKFDA LI+ LI  H VTHMCGAPVVLNML+ +   +PL 
Sbjct: 241 ANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATNEFQPLN 300

Query: 322 RPVKILTAGAPPPAAVLLRTESLGFDVSHGYGLTETGGLVIFCSWKLEWNQLPATERARL 381
           RPV ILTAGAPPPAAVLLR ES+GF +SHGYGLTET GL + C+WK +WN+LPA++RARL
Sbjct: 301 RPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRARL 360

Query: 382 KARQGVRTLAMAEADVLDSGTGKRVQRDGVSIGEIVIRGGSMMLGYFKDPEATKKAMTEE 441
           KARQGVRT+   E DV+D  +G+ V+R+G ++GEIV+RG S+MLGY KDP  T+KA+ + 
Sbjct: 361 KARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKAL-KN 420

Query: 442 GWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPDE 501
           GWF+TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLYTNPAVNE AVVARPD 
Sbjct: 421 GWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARPDV 480

Query: 502 FWGETPCAFVSLREGLTRVPTEEEIIEYCKGKLAGFMVPKTVVFMEQLPKTSTGKIQKFL 561
           FWGETPCAFVSL+ GLT+ PTE E+IEYC+ K+  +MVPKTV F+++LPKTSTGK+ KF+
Sbjct: 481 FWGETPCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMKFV 540

Query: 562 LREMAKSMGCSPKSRL 577
           LRE+AK MG +  SR+
Sbjct: 541 LREIAKKMGTTRLSRM 552

BLAST of CsGy5G013370 vs. TAIR 10
Match: AT5G16340.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 737.3 bits (1902), Expect = 9.7e-213
Identity = 359/555 (64.68%), Postives = 450/555 (81.08%), Query Frame = 0

Query: 22  MEHLKPTPANSSPLTPIGFLERASLIYADSPSIIY-SSTTFTWSQTYRRCLRLASSLSSI 81
           ME +KP  ANS PLTPIGFLERA+ +Y D  SI+Y S+T +TW +T  RCLR+ASSLSSI
Sbjct: 1   MEEMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 82  GIHKGHVVSVIAPNTPPMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQ 141
           GI +  VVSV++PNTP MYELQFAVPM+GAIL+ IN RLDART+SV LRH ESKLLFVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDV 120

Query: 142 ASSGLISDALALFPPEAKRPLLVLISDDAAVEDSASSVAIGGDVVDL------YEDMVQK 201
            S  L  +A+++   +   P+LV+I+D    ++    VA   DV DL      Y+D++++
Sbjct: 121 FSVDLAVEAVSMMTTDP--PILVVIAD----KEEEGGVA---DVADLSKFSYTYDDLIER 180

Query: 202 GDPEFRWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLW 261
           GDP F+WIRP SEWDP+V+NYTSGTTS+PKGVVH HRGIF +++DSLI+W VPK+ VYLW
Sbjct: 181 GDPGFKWIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSVDSLIDWAVPKNPVYLW 240

Query: 262 TLPMFHANGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHMCGAPVVLNMLTNSP 321
           TLP+FH+NGW+ P+ IAAVGGTN+C+RKFDA LI+ LI  H VTHMCGAPVVLNML+ + 
Sbjct: 241 TLPIFHSNGWTNPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATQ 300

Query: 322 NNRPLERPVKILTAGAPPPAAVLLRTESLGFDVSHGYGLTETGGLVIFCSWKLEWNQLPA 381
            ++PL  PV ILTAG+PPPA VLLR ES+GF +SHGYGLTET G+++ C+WK +WN LPA
Sbjct: 301 ESQPLNHPVNILTAGSPPPATVLLRAESIGFVISHGYGLTETAGVIVSCAWKPKWNHLPA 360

Query: 382 TERARLKARQGVRTLAMAEADVLDSGTGKRVQRDGVSIGEIVIRGGSMMLGYFKDPEATK 441
           ++RARLKARQGVRT+   E DV+D  +G  V+R+G ++GEIV+RG S+MLGY KDP  T+
Sbjct: 361 SDRARLKARQGVRTVGFTEIDVVDPESGLSVERNGETVGEIVMRGSSVMLGYLKDPVGTE 420

Query: 442 KAMTEEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAV 501
           KA+ + GWF+TGDVGV+H DGYLEIKDRSKD+II+GGEN+SSVEVE+VLYT PAVNE AV
Sbjct: 421 KAL-KNGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTIPAVNEVAV 480

Query: 502 VARPDEFWGETPCAFVSLREGLTRVPTEEEIIEYCKGKLAGFMVPKTVVFMEQLPKTSTG 561
           VARPDEFWGETPCAFVSL+ G +  PTEEE++EYC+ K+  +MVPKTV FM++LPK+STG
Sbjct: 481 VARPDEFWGETPCAFVSLKNGFSGKPTEEELMEYCRKKMPKYMVPKTVSFMDELPKSSTG 540

Query: 562 KIQKFLLREMAKSMG 570
           K+ KF+LR++AK MG
Sbjct: 541 KVTKFVLRDIAKKMG 545

BLAST of CsGy5G013370 vs. TAIR 10
Match: AT1G77240.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 709.1 bits (1829), Expect = 2.8e-204
Identity = 340/549 (61.93%), Postives = 437/549 (79.60%), Query Frame = 0

Query: 22  MEHLKPTPANSSPLTPIGFLERASLIYADSPSIIYSSTTFTWSQTYRRCLRLASSLS--S 81
           ME L P  +NS PLT +GFLERA+ ++ DSPS+++++T  TWS+T+ RCLR+AS+LS  S
Sbjct: 1   MELLLPHASNSCPLTVLGFLERAASVFGDSPSLLHTTTVHTWSETHSRCLRIASTLSSAS 60

Query: 82  IGIHKGHVVSVIAPNTPPMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFVD 141
           +GI++G VVSVI PN P +YELQFAVPM+GA+L+ IN RLDA  +SV LRHSESKL+FVD
Sbjct: 61  LGINRGQVVSVIGPNVPSVYELQFAVPMSGAVLNNINPRLDAHALSVLLRHSESKLVFVD 120

Query: 142 QASSGLISDALALFPPEAKRPLLVLISDDAAVEDSASSVAIGGDVVDLYEDMVQKGDPEF 201
             SS L+ +A++ F P+ +RP LV+++D     D  SS +   D +D YE  +++GD  F
Sbjct: 121 HHSSSLVLEAVS-FLPKDERPRLVILNDG---NDMPSSSSADMDFLDTYEGFMERGDLRF 180

Query: 202 RWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMF 261
           +W+RP SEW P+V+NYTSGTTSSPKGVVHSHR +F  T++SL++W +P   VYLWTLPMF
Sbjct: 181 KWVRPKSEWTPMVLNYTSGTTSSPKGVVHSHRSVFMSTINSLLDWSLPNRPVYLWTLPMF 240

Query: 262 HANGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHMCGAPVVLNMLTNSPNNRPL 321
           HANGWS+ +A AAVG  NIC+ + D   IF+LI+++ VTHMC AP+VLNMLTN P  +PL
Sbjct: 241 HANGWSYTWATAAVGARNICVTRVDVPTIFNLIDKYQVTHMCAAPMVLNMLTNHPAQKPL 300

Query: 322 ERPVKILTAGAPPPAAVLLRTESLGFDVSHGYGLTETGGLVIFCSWKLEWNQLPATERAR 381
           + PVK++TAGAPPPA V+ + E+LGFDVSHGYG+TETGGLV+ C+ K EW++L   ERA+
Sbjct: 301 QSPVKVMTAGAPPPATVISKAEALGFDVSHGYGMTETGGLVVSCALKPEWDRLEPDERAK 360

Query: 382 LKARQGVRTLAMAEADVLDSGTGKRVQRDGVSIGEIVIRGGSMMLGYFKDPEATKKAMTE 441
            K+RQG+RT   AE DV D  +GK V+ DG ++GEIV RGGS+MLGY+KDPE T  +M E
Sbjct: 361 QKSRQGIRTAVFAEVDVRDPISGKSVKHDGATVGEIVFRGGSVMLGYYKDPEGTAASMRE 420

Query: 442 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 501
           +GWF+TGD+GVMHPDGYLE+KDRSKDV+I GGEN+SS E+E+VLYTNPA+ EAAVVA+PD
Sbjct: 421 DGWFYTGDIGVMHPDGYLEVKDRSKDVVICGGENISSTELEAVLYTNPAIKEAAVVAKPD 480

Query: 502 EFWGETPCAFVSLREGLTRVPTEEEIIEYCKGKLAGFMVPKTVVFMEQLPKTSTGKIQKF 561
           + WGETPCAFVSL+     V TE EI E+CK KL  +MVP+ VVF+E+LPKTSTGKIQKF
Sbjct: 481 KMWGETPCAFVSLKYHDGSV-TEREIREFCKTKLPKYMVPRNVVFLEELPKTSTGKIQKF 540

Query: 562 LLREMAKSM 569
           LLR+MAKS+
Sbjct: 541 LLRQMAKSL 544

BLAST of CsGy5G013370 vs. TAIR 10
Match: AT1G75960.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 707.6 bits (1825), Expect = 8.2e-204
Identity = 351/549 (63.93%), Postives = 439/549 (79.96%), Query Frame = 0

Query: 22  MEHLKPTPANSSPLTPIGFLERASLIYADSPSIIY-SSTTFTWSQTYRRCLRLASSLSSI 81
           ME LKP+ ANS PLT +GFLERA+ +Y D  SI+Y +ST +TW +T  RCL +AS+LSSI
Sbjct: 1   MEDLKPSAANSLPLTLLGFLERAATVYGDCTSIVYGNSTVYTWRETNHRCLCVASALSSI 60

Query: 82  GIHKGHVVSVIAPNTPPMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFVDQ 141
           GI +  VVSV++ NTP MYELQF+VPM+GAIL+ IN RLDART+SV LRH ESKLLFVD 
Sbjct: 61  GIGRSDVVSVLSANTPEMYELQFSVPMSGAILNNINTRLDARTVSVLLRHCESKLLFVDF 120

Query: 142 ASSGLISDALALFPPEAKRPLLVLISDDAAVEDSASSVAIGGDVVDLYEDMVQKGDPEFR 201
             S L  +A+ +       P+LVLI+++   E+  + V        LY D++ +G+P+F+
Sbjct: 121 FYSDLAVEAITML---LNPPILVLIANEEE-EEGGAEVTERSKFCYLYSDLITRGNPDFK 180

Query: 202 WIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPMFH 261
           WIRP SEWDPIV+NYTSGTTSSPKGVVH HRGIF +TLDSL +W VPK  VYLWTLP+FH
Sbjct: 181 WIRPGSEWDPIVVNYTSGTTSSPKGVVHCHRGIFVMTLDSLTDWAVPKTPVYLWTLPIFH 240

Query: 262 ANGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHMCGAPVVLNMLTNS-PNNRPL 321
           ANGW++P+ IAAVGGTN+C+RK  A  I+ LI  H VTHM GAP+VL +L+ S  +++PL
Sbjct: 241 ANGWTYPWGIAAVGGTNVCVRKLHAPSIYHLIRDHGVTHMYGAPIVLQILSASQESDQPL 300

Query: 322 ERPVKILTAGAPPPAAVLLRTESLGFDVSHGYGLTETGGLVIFCSWKLEWNQLPATERAR 381
           + PV  LTAG+ PPA VLLR ESLGF VSHGYGLTET G+++ C+WK  WN+LPA+++A+
Sbjct: 301 KSPVNFLTAGSSPPATVLLRAESLGFIVSHGYGLTETAGVIVSCAWKPNWNRLPASDQAQ 360

Query: 382 LKARQGVRTLAMAEADVLDSGTGKRVQRDGVSIGEIVIRGGSMMLGYFKDPEATKKAMTE 441
           LK+RQGVRT+  +E DV+D  +G+ V+RDG ++GEIV+RG S+MLGY K+P  T+ +  +
Sbjct: 361 LKSRQGVRTVGFSEIDVVDPESGRSVERDGETVGEIVLRGSSIMLGYLKNPIGTQNSF-K 420

Query: 442 EGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARPD 501
            GWFFTGD+GV+H DGYLEIKDRSKDVIISGGEN+SSVEVE+VLYTNPAVNEAAVVARPD
Sbjct: 421 NGWFFTGDLGVIHGDGYLEIKDRSKDVIISGGENVSSVEVEAVLYTNPAVNEAAVVARPD 480

Query: 502 EFWGETPCAFVSLREGLTRVPTEEEIIEYCKGKLAGFMVPKTVVFMEQLPKTSTGKIQKF 561
           EFWGETPCAFVSL+ GLTR PT++EIIEYCK K+  +M PKTV F+E+LPKTSTGKI K 
Sbjct: 481 EFWGETPCAFVSLKPGLTRKPTDKEIIEYCKYKMPRYMAPKTVSFLEELPKTSTGKIIKS 540

Query: 562 LLREMAKSM 569
           LL+E+AK+M
Sbjct: 541 LLKEIAKNM 544

BLAST of CsGy5G013370 vs. TAIR 10
Match: AT1G21540.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 707.2 bits (1824), Expect = 1.1e-203
Identity = 339/552 (61.41%), Postives = 436/552 (78.99%), Query Frame = 0

Query: 22  MEHLKPTPANSSPLTPIGFLERASLIYADSPSIIY-SSTTFTWSQTYRRCLRLASSL--S 81
           ME L P P+NS+PLT +GFL+RA+ +Y D PSI++ ++T  TWS+T+ RCLR+AS+L  S
Sbjct: 1   MELLLPHPSNSTPLTVLGFLDRAASVYGDCPSILHTTNTVHTWSETHNRCLRIASALTSS 60

Query: 82  SIGIHKGHVVSVIAPNTPPMYELQFAVPMAGAILSCINLRLDARTISVQLRHSESKLLFV 141
           S+GI++G VVSV+ PN P +YELQFAVPM+GAIL+ IN RLDA  +SV LRHSESKL+FV
Sbjct: 61  SLGINRGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSVLLRHSESKLVFV 120

Query: 142 DQASSGLISDALALFPPEAKRPLLVLISDDAAVEDSASSVAIGGDVVDLYEDMVQKGDPE 201
           D  S  ++ +A++ F  + ++P LVL+ DD   ED + S +   D +D Y+ ++++GD  
Sbjct: 121 DPNSISVVLEAVS-FMRQNEKPHLVLLDDDQ--EDGSLSPSAASDFLDTYQGVMERGDSR 180

Query: 202 FRWIRPVSEWDPIVMNYTSGTTSSPKGVVHSHRGIFTVTLDSLIEWGVPKHSVYLWTLPM 261
           F+WIRP +EW P+++NYTSGTTSSPKGVV SHR IF +T+ SL++W  P   VYLWTLPM
Sbjct: 181 FKWIRPQTEWQPMILNYTSGTTSSPKGVVLSHRAIFMLTVSSLLDWHFPNRPVYLWTLPM 240

Query: 262 FHANGWSFPFAIAAVGGTNICIRKFDAALIFSLINRHHVTHMCGAPVVLNMLTNSPNNRP 321
           FHANGW + +  AAVG TN+C R+ DA  I+ LI++HHVTHMC AP+VLNMLTN P+ +P
Sbjct: 241 FHANGWGYTWGTAAVGATNVCTRRVDAPTIYDLIDKHHVTHMCAAPMVLNMLTNYPSRKP 300

Query: 322 LERPVKILTAGAPPPAAVLLRTESLGFDVSHGYGLTETGGLVIFCSWKLEWNQLPATERA 381
           L+ PV+++TAGAPPPAA++ R E+LGF+V HGYGLTETGG V+ C+WK EW+ L   ERA
Sbjct: 301 LKNPVQVMTAGAPPPAAIISRAETLGFNVGHGYGLTETGGPVVSCAWKAEWDHLDPLERA 360

Query: 382 RLKARQGVRTLAMAEADVLDSGTGKRVQRDGVSIGEIVIRGGSMMLGYFKDPEATKKAMT 441
           RLK+RQGVRT+  AE DV D  TGK V+ DGVS+GEIV++GGS+MLGY+KDPE T   M 
Sbjct: 361 RLKSRQGVRTIGFAEVDVRDPRTGKSVEHDGVSVGEIVLKGGSVMLGYYKDPEGTAACMR 420

Query: 442 EEGWFFTGDVGVMHPDGYLEIKDRSKDVIISGGENLSSVEVESVLYTNPAVNEAAVVARP 501
           E+GWF++GDVGV+H DGYLE+KDRSKDVII GGEN+SS EVE+VLYTNP V EAAVVA+P
Sbjct: 421 EDGWFYSGDVGVIHEDGYLEVKDRSKDVIICGGENISSAEVETVLYTNPVVKEAAVVAKP 480

Query: 502 DEFWGETPCAFVSLR--EGLTRVPTEEEIIEYCKGKLAGFMVPKTVVFMEQLPKTSTGKI 561
           D+ WGETPCAFVSL+       + TE EI E+CK +L  +MVP+ V+F E+LPKTSTGKI
Sbjct: 481 DKMWGETPCAFVSLKYDSNGNGLVTEREIREFCKTRLPKYMVPRKVIFQEELPKTSTGKI 540

Query: 562 QKFLLREMAKSM 569
           QKFLLR+MAKS+
Sbjct: 541 QKFLLRQMAKSL 549