CsGy5G011625 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy5G011625
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Descriptioncalmodulin-like
LocationGy14Chr5: 12813383 .. 12813905 (+)
RNA-Seq ExpressionCsGy5G011625
SyntenyCsGy5G011625
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTACATCTCCAAACTTCAAGCTTCCATGGCTATGTATGAAGCAAGTATGTTGATCAAGCAGTATGAAAAAAATGGTGATGCTGTTAAAGTAAAAGCTTTCGATGATGTTAGAAGTTCAACTCAAATTGGCAATCGAGAAAGTGTACTGAAAATCCTAAAACCTAGTGACGGTGTTGCACACAAGCTTCGTTCAGAAGCACATATACAACCAACTCAGCGAACTGGAGGAAAGGGTAAAAAGTCCATCACAAGGGTTCTTGACAGAAAGTCATCTAATATGTCACTTAGTCGAGAGCGAATAAAGGAAATTTTCCAATATCATGATAACAACAATGATGGTTTTCTTAACAGAATGGAACTCACTAAAGCTTTCGCATTTTTAGGCTCAATGTTTCCATTTTACAAAGCTTGCTACGGAATGGTATATGCAGATGCAAATGAAGATGGTCTTATCAGTGAAGCTGAGCTTGACAAACTCATTGATTATGCGAGTAAAATTATCAAGAAGAAATAACTTTTAG

mRNA sequence

ATGTACATCTCCAAACTTCAAGCTTCCATGGCTATGTATGAAGCAAGTATGTTGATCAAGCAGTATGAAAAAAATGGTGATGCTGTTAAAGTAAAAGCTTTCGATGATGTTAGAAGTTCAACTCAAATTGGCAATCGAGAAAGTGTACTGAAAATCCTAAAACCTAGTGACGGTGTTGCACACAAGCTTCGTTCAGAAGCACATATACAACCAACTCAGCGAACTGGAGGAAAGGGTAAAAAGTCCATCACAAGGGTTCTTGACAGAAAGTCATCTAATATGTCACTTAGTCGAGAGCGAATAAAGGAAATTTTCCAATATCATGATAACAACAATGATGGTTTTCTTAACAGAATGGAACTCACTAAAGCTTTCGCATTTTTAGGCTCAATGTTTCCATTTTACAAAGCTTGCTACGGAATGGTATATGCAGATGCAAATGAAGATGGTCTTATCAGTGAAGCTGAGCTTGACAAACTCATTGATTATGCGAGTAAAATTATCAAGAAGAAATAACTTTTAG

Coding sequence (CDS)

ATGTACATCTCCAAACTTCAAGCTTCCATGGCTATGTATGAAGCAAGTATGTTGATCAAGCAGTATGAAAAAAATGGTGATGCTGTTAAAGTAAAAGCTTTCGATGATGTTAGAAGTTCAACTCAAATTGGCAATCGAGAAAGTGTACTGAAAATCCTAAAACCTAGTGACGGTGTTGCACACAAGCTTCGTTCAGAAGCACATATACAACCAACTCAGCGAACTGGAGGAAAGGGTAAAAAGTCCATCACAAGGGTTCTTGACAGAAAGTCATCTAATATGTCACTTAGTCGAGAGCGAATAAAGGAAATTTTCCAATATCATGATAACAACAATGATGGTTTTCTTAACAGAATGGAACTCACTAAAGCTTTCGCATTTTTAGGCTCAATGTTTCCATTTTACAAAGCTTGCTACGGAATGGTATATGCAGATGCAAATGAAGATGGTCTTATCAGTGAAGCTGAGCTTGACAAACTCATTGATTATGCGAGTAAAATTATCAAGAAGAAATAA

Protein sequence

MYISKLQASMAMYEASMLIKQYEKNGDAVKVKAFDDVRSSTQIGNRESVLKILKPSDGVAHKLRSEAHIQPTQRTGGKGKKSITRVLDRKSSNMSLSRERIKEIFQYHDNNNDGFLNRMELTKAFAFLGSMFPFYKACYGMVYADANEDGLISEAELDKLIDYASKIIKKK*
Homology
BLAST of CsGy5G011625 vs. NCBI nr
Match: KAE8648233.1 (hypothetical protein Csa_018371 [Cucumis sativus])

HSP 1 Score: 303 bits (775), Expect = 3.69e-103
Identity = 155/155 (100.00%), Postives = 155/155 (100.00%), Query Frame = 0

Query: 10  MAMYEASMLIKQYEKNGDAVKVKAFDDVRSSTQIGNRESVLKILKPSDGVAHKLRSEAHI 69
           MAMYEASMLIKQYEKNGDAVKVKAFDDVRSSTQIGNRESVLKILKPSDGVAHKLRSEAHI
Sbjct: 1   MAMYEASMLIKQYEKNGDAVKVKAFDDVRSSTQIGNRESVLKILKPSDGVAHKLRSEAHI 60

Query: 70  QPTQRTGGKGKKSITRVLDRKSSNMSLSRERIKEIFQYHDNNNDGFLNRMELTKAFAFLG 129
           QPTQRTGGKGKKSITRVLDRKSSNMSLSRERIKEIFQYHDNNNDGFLNRMELTKAFAFLG
Sbjct: 61  QPTQRTGGKGKKSITRVLDRKSSNMSLSRERIKEIFQYHDNNNDGFLNRMELTKAFAFLG 120

Query: 130 SMFPFYKACYGMVYADANEDGLISEAELDKLIDYA 164
           SMFPFYKACYGMVYADANEDGLISEAELDKLIDYA
Sbjct: 121 SMFPFYKACYGMVYADANEDGLISEAELDKLIDYA 155

BLAST of CsGy5G011625 vs. NCBI nr
Match: KAA0065465.1 (putative calcium-binding protein CML11 [Cucumis melo var. makuwa])

HSP 1 Score: 273 bits (699), Expect = 2.14e-91
Identity = 143/171 (83.63%), Postives = 153/171 (89.47%), Query Frame = 0

Query: 1   MYISKLQASMAMYEASMLIKQYEKNGDAVKVKAFDDVRSSTQIGNRESVLKILKPSDGVA 60
           MYISKLQASMAMYEA M IK YEK+GDAV+VK F+DVRSSTQIG +  V KILK SD VA
Sbjct: 1   MYISKLQASMAMYEARMFIKHYEKDGDAVQVKGFNDVRSSTQIGYQGIVPKILKSSDDVA 60

Query: 61  HKLRSEAHIQPTQRTGGKGKKSITRVLDRKSSNMSLSRERIKEIFQYHDNNNDGFLNRME 120
           HKL  EAHIQPTQ+TGGKGKKSI R LD KSSNMS SR++IKEIF+YHDNNNDGFLN +E
Sbjct: 61  HKLSLEAHIQPTQQTGGKGKKSIARALDIKSSNMSFSRQQIKEIFRYHDNNNDGFLNIVE 120

Query: 121 LTKAFAFLGSMFPFYKACYGMVYADANEDGLISEAELDKLIDYASKIIKKK 171
           LTKAFAFLGSMFPFYKACYGMVYADAN DGLISEAELDKLIDYA+KIIKKK
Sbjct: 121 LTKAFAFLGSMFPFYKACYGMVYADANADGLISEAELDKLIDYATKIIKKK 171

BLAST of CsGy5G011625 vs. NCBI nr
Match: XP_022930007.1 (uncharacterized protein LOC111436449 [Cucurbita moschata])

HSP 1 Score: 177 bits (450), Expect = 8.97e-54
Identity = 99/172 (57.56%), Postives = 121/172 (70.35%), Query Frame = 0

Query: 1   MYISKLQASMAMYEASMLIKQYEKNGDAVKVKAFDDV-RSSTQIGNRESVLKILKPSDGV 60
           M+ISKLQASMA YEAS LIK+Y +NGD V++K+F D+ R STQ+ N+  + +ILK +  V
Sbjct: 1   MFISKLQASMAKYEASELIKKYPRNGDDVQLKSFSDIARDSTQLNNQGKIPEILKDNKNV 60

Query: 61  AHKLRSEAHIQPTQRTGGKGKKSITRVLDRKSSNMSLSRERIKEIFQYHDNNNDGFLNRM 120
             KL S AH                     K+S +  SRE++KEIF+YHD++ DGFLN  
Sbjct: 61  GLKLSSNAHP--------------------KTSKLQFSREQMKEIFKYHDSDKDGFLNLR 120

Query: 121 ELTKAFAFLGSMFPFYKACYGMVYADANEDGLISEAELDKLIDYASKIIKKK 171
           ELTKAFAFLGS+ PF KA YGM YADAN+DGLISEAELDKLIDYA K +KKK
Sbjct: 121 ELTKAFAFLGSIIPFEKASYGMSYADANKDGLISEAELDKLIDYAQKFVKKK 152

BLAST of CsGy5G011625 vs. NCBI nr
Match: XP_023530717.1 (calmodulin-2/4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 176 bits (445), Expect = 5.15e-53
Identity = 98/172 (56.98%), Postives = 120/172 (69.77%), Query Frame = 0

Query: 1   MYISKLQASMAMYEASMLIKQYEKNGDAVKVKAFDDV-RSSTQIGNRESVLKILKPSDGV 60
           M+ISKLQASMA YEAS LIK+Y +NGD V++K+F  + R STQ+ N+  + +ILK +  V
Sbjct: 1   MFISKLQASMAKYEASELIKKYPRNGDDVQLKSFSGIARDSTQLNNQGKIPEILKDNKNV 60

Query: 61  AHKLRSEAHIQPTQRTGGKGKKSITRVLDRKSSNMSLSRERIKEIFQYHDNNNDGFLNRM 120
             KL S AH                     K+S +  SRE++KEIF+YHD++ DGFLN  
Sbjct: 61  GLKLSSNAHP--------------------KTSKLQFSREQMKEIFRYHDSDKDGFLNLR 120

Query: 121 ELTKAFAFLGSMFPFYKACYGMVYADANEDGLISEAELDKLIDYASKIIKKK 171
           ELTKAFAFLGS+ PF KA YGM YADAN+DGLISEAELDKLIDYA K +KKK
Sbjct: 121 ELTKAFAFLGSIIPFEKASYGMTYADANKDGLISEAELDKLIDYAQKFVKKK 152

BLAST of CsGy5G011625 vs. NCBI nr
Match: XP_023530727.1 (calmodulin-like protein 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 174 bits (442), Expect = 1.47e-52
Identity = 97/172 (56.40%), Postives = 121/172 (70.35%), Query Frame = 0

Query: 1   MYISKLQASMAMYEASMLIKQYEKNGDAVKVKAFDDV-RSSTQIGNRESVLKILKPSDGV 60
           M+ISKLQASMA YEAS +IK++ +NGD V++++F  + R STQ+ N+  +L+ILK +  V
Sbjct: 1   MFISKLQASMAKYEASEMIKKHIRNGDNVQLQSFSGIARDSTQLNNQGKILEILKDNKNV 60

Query: 61  AHKLRSEAHIQPTQRTGGKGKKSITRVLDRKSSNMSLSRERIKEIFQYHDNNNDGFLNRM 120
             KL S AH                     K+S +  SRE++KEIF+YHDN+ DGFLN  
Sbjct: 61  GLKLSSNAHP--------------------KTSKLQFSREQMKEIFRYHDNDKDGFLNLR 120

Query: 121 ELTKAFAFLGSMFPFYKACYGMVYADANEDGLISEAELDKLIDYASKIIKKK 171
           ELTKAFAFLGS+ PF KA YGM YADAN+DGLISEAELDKLIDYA K +KKK
Sbjct: 121 ELTKAFAFLGSIIPFEKASYGMSYADANKDGLISEAELDKLIDYAQKFVKKK 152

BLAST of CsGy5G011625 vs. ExPASy TrEMBL
Match: A0A0A0KMQ1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G067410 PE=4 SV=1)

HSP 1 Score: 330 bits (847), Expect = 2.85e-114
Identity = 171/171 (100.00%), Postives = 171/171 (100.00%), Query Frame = 0

Query: 1   MYISKLQASMAMYEASMLIKQYEKNGDAVKVKAFDDVRSSTQIGNRESVLKILKPSDGVA 60
           MYISKLQASMAMYEASMLIKQYEKNGDAVKVKAFDDVRSSTQIGNRESVLKILKPSDGVA
Sbjct: 1   MYISKLQASMAMYEASMLIKQYEKNGDAVKVKAFDDVRSSTQIGNRESVLKILKPSDGVA 60

Query: 61  HKLRSEAHIQPTQRTGGKGKKSITRVLDRKSSNMSLSRERIKEIFQYHDNNNDGFLNRME 120
           HKLRSEAHIQPTQRTGGKGKKSITRVLDRKSSNMSLSRERIKEIFQYHDNNNDGFLNRME
Sbjct: 61  HKLRSEAHIQPTQRTGGKGKKSITRVLDRKSSNMSLSRERIKEIFQYHDNNNDGFLNRME 120

Query: 121 LTKAFAFLGSMFPFYKACYGMVYADANEDGLISEAELDKLIDYASKIIKKK 171
           LTKAFAFLGSMFPFYKACYGMVYADANEDGLISEAELDKLIDYASKIIKKK
Sbjct: 121 LTKAFAFLGSMFPFYKACYGMVYADANEDGLISEAELDKLIDYASKIIKKK 171

BLAST of CsGy5G011625 vs. ExPASy TrEMBL
Match: A0A5A7VI66 (Putative calcium-binding protein CML11 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold17G001550 PE=4 SV=1)

HSP 1 Score: 273 bits (699), Expect = 1.04e-91
Identity = 143/171 (83.63%), Postives = 153/171 (89.47%), Query Frame = 0

Query: 1   MYISKLQASMAMYEASMLIKQYEKNGDAVKVKAFDDVRSSTQIGNRESVLKILKPSDGVA 60
           MYISKLQASMAMYEA M IK YEK+GDAV+VK F+DVRSSTQIG +  V KILK SD VA
Sbjct: 1   MYISKLQASMAMYEARMFIKHYEKDGDAVQVKGFNDVRSSTQIGYQGIVPKILKSSDDVA 60

Query: 61  HKLRSEAHIQPTQRTGGKGKKSITRVLDRKSSNMSLSRERIKEIFQYHDNNNDGFLNRME 120
           HKL  EAHIQPTQ+TGGKGKKSI R LD KSSNMS SR++IKEIF+YHDNNNDGFLN +E
Sbjct: 61  HKLSLEAHIQPTQQTGGKGKKSIARALDIKSSNMSFSRQQIKEIFRYHDNNNDGFLNIVE 120

Query: 121 LTKAFAFLGSMFPFYKACYGMVYADANEDGLISEAELDKLIDYASKIIKKK 171
           LTKAFAFLGSMFPFYKACYGMVYADAN DGLISEAELDKLIDYA+KIIKKK
Sbjct: 121 LTKAFAFLGSMFPFYKACYGMVYADANADGLISEAELDKLIDYATKIIKKK 171

BLAST of CsGy5G011625 vs. ExPASy TrEMBL
Match: A0A0A0KLF3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G067670 PE=4 SV=1)

HSP 1 Score: 189 bits (479), Expect = 7.86e-58
Identity = 104/173 (60.12%), Postives = 129/173 (74.57%), Query Frame = 0

Query: 1   MYISKLQASMAMYEASMLIKQYEKNGDAVKVKAFDD-VRSSTQIG-NRESVLKILKPSDG 60
           M+I +LQ S A YEAS L+K+YE+NG+ V + AF+D  R STQI  NRE   K LKP+D 
Sbjct: 28  MHIPQLQMSKAKYEASKLMKKYEQNGEGVILSAFNDNARGSTQINTNREMATKKLKPNDD 87

Query: 61  VAHKLRSEAHIQPTQRTGGKGKKSITRVLDRKSSNMSLSRERIKEIFQYHDNNNDGFLNR 120
           VAHKL S A ++   +  GK +  I RV D K+SNM  SRE+IKEIFQY+D++ DGFLN 
Sbjct: 88  VAHKLSSNAQLKSNNQIRGKAQVPIKRVYDTKASNMQFSREQIKEIFQYYDSDRDGFLNI 147

Query: 121 MELTKAFAFLGSMFPFYKACYGMVYADANEDGLISEAELDKLIDYASKIIKKK 171
            E+TKAFA LGS+FPF KA +GMVYAD N+DGLI E ELDKLI+YA+K +KKK
Sbjct: 148 REVTKAFALLGSIFPFNKAYHGMVYADTNKDGLIGEDELDKLINYANKFMKKK 200

BLAST of CsGy5G011625 vs. ExPASy TrEMBL
Match: A0A6J1EP67 (uncharacterized protein LOC111436449 OS=Cucurbita moschata OX=3662 GN=LOC111436449 PE=4 SV=1)

HSP 1 Score: 177 bits (450), Expect = 4.34e-54
Identity = 99/172 (57.56%), Postives = 121/172 (70.35%), Query Frame = 0

Query: 1   MYISKLQASMAMYEASMLIKQYEKNGDAVKVKAFDDV-RSSTQIGNRESVLKILKPSDGV 60
           M+ISKLQASMA YEAS LIK+Y +NGD V++K+F D+ R STQ+ N+  + +ILK +  V
Sbjct: 1   MFISKLQASMAKYEASELIKKYPRNGDDVQLKSFSDIARDSTQLNNQGKIPEILKDNKNV 60

Query: 61  AHKLRSEAHIQPTQRTGGKGKKSITRVLDRKSSNMSLSRERIKEIFQYHDNNNDGFLNRM 120
             KL S AH                     K+S +  SRE++KEIF+YHD++ DGFLN  
Sbjct: 61  GLKLSSNAHP--------------------KTSKLQFSREQMKEIFKYHDSDKDGFLNLR 120

Query: 121 ELTKAFAFLGSMFPFYKACYGMVYADANEDGLISEAELDKLIDYASKIIKKK 171
           ELTKAFAFLGS+ PF KA YGM YADAN+DGLISEAELDKLIDYA K +KKK
Sbjct: 121 ELTKAFAFLGSIIPFEKASYGMSYADANKDGLISEAELDKLIDYAQKFVKKK 152

BLAST of CsGy5G011625 vs. ExPASy TrEMBL
Match: A0A0A0KIY4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G068170 PE=4 SV=1)

HSP 1 Score: 178 bits (452), Expect = 4.62e-54
Identity = 100/176 (56.82%), Postives = 134/176 (76.14%), Query Frame = 0

Query: 1   MYISKLQASMAMYEASMLIKQYEKNGDAVKVKAFDDVR-SSTQIGNRESVLK--ILKPSD 60
           MYIS LQ S A YEAS L+K++E N + V +KAF+ +  +ST+I N++++    ILKPS+
Sbjct: 1   MYISNLQVSKAKYEASKLMKKHENNDEGVPLKAFNGIPPASTKINNKQAMATEIILKPSN 60

Query: 61  G-VAHKLRSEAHIQPTQRTG-GKGKKSITRVLDRKSSNMSLSRERIKEIFQYHDNNNDGF 120
             VAH L S A++ P+++ G GK + + T VLD K+SNM  SRE+IKEIFQYHD++NDGF
Sbjct: 61  HPVAHYLTSNAYMIPSKQLGAGKAQVTTTSVLDIKASNMQFSREQIKEIFQYHDSDNDGF 120

Query: 121 LNRMELTKAFAFLGSMFPFYKACYGMVYADANEDGLISEAELDKLIDYASKIIKKK 171
           LN  E+TKAFAFLG++ P YKA +GMVYADAN+DGLIS+ EL+KL+DYA K   K+
Sbjct: 121 LNLREVTKAFAFLGTILPLYKAYHGMVYADANKDGLISKDELEKLVDYAYKFFNKR 176

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
KAE8648233.13.69e-103100.00hypothetical protein Csa_018371 [Cucumis sativus][more]
KAA0065465.12.14e-9183.63putative calcium-binding protein CML11 [Cucumis melo var. makuwa][more]
XP_022930007.18.97e-5457.56uncharacterized protein LOC111436449 [Cucurbita moschata][more]
XP_023530717.15.15e-5356.98calmodulin-2/4-like [Cucurbita pepo subsp. pepo][more]
XP_023530727.11.47e-5256.40calmodulin-like protein 1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A0A0KMQ12.85e-114100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G067410 PE=4 SV=1[more]
A0A5A7VI661.04e-9183.63Putative calcium-binding protein CML11 OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A0A0KLF37.86e-5860.12Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G067670 PE=4 SV=1[more]
A0A6J1EP674.34e-5457.56uncharacterized protein LOC111436449 OS=Cucurbita moschata OX=3662 GN=LOC1114364... [more]
A0A0A0KIY44.62e-5456.82Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G068170 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 144..160
e-value: 0.13
score: 11.8
IPR002048EF-hand domainPFAMPF13405EF-hand_6coord: 101..127
e-value: 2.2E-5
score: 23.9
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 96..131
score: 12.700205
NoneNo IPR availableGENE3D1.10.238.10coord: 59..161
e-value: 1.5E-9
score: 39.8
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 109..121
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 145..157
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 94..159

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy5G011625.1CsGy5G011625.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding