CsGy5G009470 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy5G009470
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionPectinesterase
LocationGy14Chr5: 8047837 .. 8048931 (+)
RNA-Seq ExpressionCsGy5G009470
SyntenyCsGy5G009470
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAAATTTGAAAGTAGAGACAATTATTTTGTTGTTCTTTTTCAATCTCCTCCTATACATCCAATCTTTCACTTTCAATGCAACGGTGTCTTTAGATGGTTCCATAGATCATTTTAAGACTATTACTGATGCAATTGCATCAGTCCCAAATAACTTAAACACACGATTTTATATTCATGTAACTCCTGGTACTTACCATGAGTGTCTTCAAATCCCACCAACCAAGACCTTCATCGCTTTGATCGGCGATAATGCACTCACCACCATTATTGTTGATGATAGAAGTAATGCTCGTGGATTCAAGACAATTGACTCTGCAACTCTTAGTGAGTGTCATTCAATTTCTTTTTTTATTATGCCTTTTCACAAATACATACTACTTTACTAACCATTTTTCTTTCACGCAGCGGTTAATGGTAATAATTTTCTTGCACAATCATTGACATTTAAGAACTCCGCTGGTCCTCAAAATGGCCAAGCAGTGGCAGTTCTTGACGAAGCTCACTTCACTACGTACTACAAGTGCAGGTTCTTAGGTTTTCAAGACACTTTGTACGTCAGAGGGAAACCTCAATTCTTCAAAGAGTGTGATATCTATGGCAGTGTCGACTTTATTTTTGGAGATGGCTTAGTTATGTTTCAAGATTGTAACATCTATGCCCGAGAACCAAACAGGTCTATCACAATCACTGCTCAATCAAAAAAACAATTACACGAAGAAAGCGGCTTTTCATTTCAAAATTGTACTATCACTATTTCTTCGGAGATAGCAGCCAACAAAGCCGACGTCAAGATCTATCTTGGCCGACCGTGGAGACAATATTCACAGGTTGTGTTCATGGAGTCATTTCTTGATAAGGAGGTGATGCCTAAAGGATGGTTAAAGTGGTCAGGGGTACCGTTGAACAATTTGTTTTACGGAGAGTTTAATAACCGTGGTCCTGGGGCAGATGTTTCTAAAAGAATTCACTGTACTGGGTTTCATGTGTTAGATAAGCAGTCAGCCAATCAATTTACTATAAACTTTGTTAATGGATCTGACTGGTTACCTGAAACTGGTGTTCCATTTAGAAGTGGACTTTTTTCTGAATGA

mRNA sequence

ATGAAAAATTTGAAAGTAGAGACAATTATTTTGTTGTTCTTTTTCAATCTCCTCCTATACATCCAATCTTTCACTTTCAATGCAACGGTGTCTTTAGATGGTTCCATAGATCATTTTAAGACTATTACTGATGCAATTGCATCAGTCCCAAATAACTTAAACACACGATTTTATATTCATGTAACTCCTGGTACTTACCATGAGTGTCTTCAAATCCCACCAACCAAGACCTTCATCGCTTTGATCGGCGATAATGCACTCACCACCATTATTGTTGATGATAGAAGTAATGCTCGTGGATTCAAGACAATTGACTCTGCAACTCTTACGGTTAATGGTAATAATTTTCTTGCACAATCATTGACATTTAAGAACTCCGCTGGTCCTCAAAATGGCCAAGCAGTGGCAGTTCTTGACGAAGCTCACTTCACTACGTACTACAAGTGCAGGTTCTTAGGTTTTCAAGACACTTTGTACGTCAGAGGGAAACCTCAATTCTTCAAAGAGTGTGATATCTATGGCAGTGTCGACTTTATTTTTGGAGATGGCTTAGTTATGTTTCAAGATTGTAACATCTATGCCCGAGAACCAAACAGGTCTATCACAATCACTGCTCAATCAAAAAAACAATTACACGAAGAAAGCGGCTTTTCATTTCAAAATTGTACTATCACTATTTCTTCGGAGATAGCAGCCAACAAAGCCGACGTCAAGATCTATCTTGGCCGACCGTGGAGACAATATTCACAGGTTGTGTTCATGGAGTCATTTCTTGATAAGGAGGTGATGCCTAAAGGATGGTTAAAGTGGTCAGGGGTACCGTTGAACAATTTGTTTTACGGAGAGTTTAATAACCGTGGTCCTGGGGCAGATGTTTCTAAAAGAATTCACTGTACTGGGTTTCATGTGTTAGATAAGCAGTCAGCCAATCAATTTACTATAAACTTTGTTAATGGATCTGACTGGTTACCTGAAACTGGTGTTCCATTTAGAAGTGGACTTTTTTCTGAATGA

Coding sequence (CDS)

ATGAAAAATTTGAAAGTAGAGACAATTATTTTGTTGTTCTTTTTCAATCTCCTCCTATACATCCAATCTTTCACTTTCAATGCAACGGTGTCTTTAGATGGTTCCATAGATCATTTTAAGACTATTACTGATGCAATTGCATCAGTCCCAAATAACTTAAACACACGATTTTATATTCATGTAACTCCTGGTACTTACCATGAGTGTCTTCAAATCCCACCAACCAAGACCTTCATCGCTTTGATCGGCGATAATGCACTCACCACCATTATTGTTGATGATAGAAGTAATGCTCGTGGATTCAAGACAATTGACTCTGCAACTCTTACGGTTAATGGTAATAATTTTCTTGCACAATCATTGACATTTAAGAACTCCGCTGGTCCTCAAAATGGCCAAGCAGTGGCAGTTCTTGACGAAGCTCACTTCACTACGTACTACAAGTGCAGGTTCTTAGGTTTTCAAGACACTTTGTACGTCAGAGGGAAACCTCAATTCTTCAAAGAGTGTGATATCTATGGCAGTGTCGACTTTATTTTTGGAGATGGCTTAGTTATGTTTCAAGATTGTAACATCTATGCCCGAGAACCAAACAGGTCTATCACAATCACTGCTCAATCAAAAAAACAATTACACGAAGAAAGCGGCTTTTCATTTCAAAATTGTACTATCACTATTTCTTCGGAGATAGCAGCCAACAAAGCCGACGTCAAGATCTATCTTGGCCGACCGTGGAGACAATATTCACAGGTTGTGTTCATGGAGTCATTTCTTGATAAGGAGGTGATGCCTAAAGGATGGTTAAAGTGGTCAGGGGTACCGTTGAACAATTTGTTTTACGGAGAGTTTAATAACCGTGGTCCTGGGGCAGATGTTTCTAAAAGAATTCACTGTACTGGGTTTCATGTGTTAGATAAGCAGTCAGCCAATCAATTTACTATAAACTTTGTTAATGGATCTGACTGGTTACCTGAAACTGGTGTTCCATTTAGAAGTGGACTTTTTTCTGAATGA

Protein sequence

MKNLKVETIILLFFFNLLLYIQSFTFNATVSLDGSIDHFKTITDAIASVPNNLNTRFYIHVTPGTYHECLQIPPTKTFIALIGDNALTTIIVDDRSNARGFKTIDSATLTVNGNNFLAQSLTFKNSAGPQNGQAVAVLDEAHFTTYYKCRFLGFQDTLYVRGKPQFFKECDIYGSVDFIFGDGLVMFQDCNIYAREPNRSITITAQSKKQLHEESGFSFQNCTITISSEIAANKADVKIYLGRPWRQYSQVVFMESFLDKEVMPKGWLKWSGVPLNNLFYGEFNNRGPGADVSKRIHCTGFHVLDKQSANQFTINFVNGSDWLPETGVPFRSGLFSE*
Homology
BLAST of CsGy5G009470 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 285.8 bits (730), Expect = 6.3e-76
Identity = 137/312 (43.91%), Postives = 206/312 (66.03%), Query Frame = 0

Query: 27  NATVSLDGSIDHFKTITDAIASVPNNLNTRFYIHVTPGTYHECLQIPPTKTFIALIGDNA 86
           N  V+ DGS  + KTI +A+A+      +R+ I++  GTY+E +++      I  +GD  
Sbjct: 202 NIVVAQDGS-GNVKTIQEAVAAASRAGGSRYVIYIKAGTYNENIEVKLKN--IMFVGDGI 261

Query: 87  LTTIIVDDRSNARGFKTIDSATLTVNGNNFLAQSLTFKNSAGPQNGQAVAVLDEAHFTTY 146
             TII   +S   G  T  SAT+ V G+NF+A+ +T +N+AGP N QAVA+   +  + +
Sbjct: 262 GKTIITGSKSVGGGATTFKSATVAVVGDNFIARDITIRNTAGPNNHQAVALRSGSDLSVF 321

Query: 147 YKCRFLGFQDTLYVRGKPQFFKECDIYGSVDFIFGDGLVMFQDCNIYARE-PNRSITITA 206
           Y+C F G+QDTLYV  + QF++ECDIYG+VDFIFG+  V+ Q+CNI+AR+ PNR+ T+TA
Sbjct: 322 YRCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQNCNIFARKPPNRTNTLTA 381

Query: 207 QSKKQLHEESGFSFQNCTITISSEIAANKADVKIYLGRPWRQYSQVVFMESFLDKEVMPK 266
           Q +   ++ +G    NC +T +S++   ++ VK +LGRPW+QYS+ V++++FLD  + P 
Sbjct: 382 QGRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGRPWKQYSRTVYIKTFLDSLINPA 441

Query: 267 GWLKWSG-VPLNNLFYGEFNNRGPGADVSKRIHCTGFHVLDKQS-ANQFTI-NFVNGSDW 326
           GW++WSG   LN L+Y E+ N GPG+  + R+   G+HVL   S  +QFT+ NF+ G+ W
Sbjct: 442 GWMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQVSQFTVGNFIAGNSW 501

Query: 327 LPETGVPFRSGL 335
           LP T VPF SGL
Sbjct: 502 LPATNVPFTSGL 510

BLAST of CsGy5G009470 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 1.1e-75
Identity = 137/316 (43.35%), Postives = 206/316 (65.19%), Query Frame = 0

Query: 29  TVSLDGSIDHFKTITDAIASVPNNLNTR---FYIHVTPGTYHECLQIPPTKTFIALIGDN 88
           TVS DG+  +F  IT A+A+ PNN +     F I+VT G Y E + I   K ++ +IGD 
Sbjct: 266 TVSQDGT-GNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEEYISIAKNKRYMMMIGDG 325

Query: 89  ALTTIIVDDRSNARGFKTIDSATLTVNGNNFLAQSLTFKNSAGPQNGQAVAVLDEAHFTT 148
              T++  +RS   G+ T +SAT  V   NF+A ++TF+N+AGP+  QAVA+   A F+ 
Sbjct: 326 INQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNTAGPEKHQAVALRSGADFSI 385

Query: 149 YYKCRFLGFQDTLYVRGKPQFFKECDIYGSVDFIFGDGLVMFQDCNIYARE--PNRSITI 208
           +Y C F  +QDTLY     QF++ECD+YG+VDFIFG+  V+FQ+CN+Y R+  PN+   I
Sbjct: 386 FYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYPRKPMPNQFNAI 445

Query: 209 TAQSKKQLHEESGFSFQNCTITISSEIAANKADVKIYLGRPWRQYSQVVFMESFLDKEVM 268
           TAQ +   ++ +G S QNCTI  + ++ ++   VK YLGRPW++YS+ V+M+S++D  V 
Sbjct: 446 TAQGRSDPNQNTGTSIQNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVYMQSYIDGFVE 505

Query: 269 PKGWLKWSG-VPLNNLFYGEFNNRGPGADVSKRIHCTGFHVLDKQSANQFTIN--FVNGS 328
           P GW +W+G   L+ L+Y E+NN GPG++ + R+   G+HV++   A  FT+   F+  +
Sbjct: 506 PVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVINSTDAANFTVTGLFIE-A 565

Query: 329 DWLPETGVPFRSGLFS 337
           DW+ +TGVP+ SGL S
Sbjct: 566 DWIWKTGVPYTSGLIS 579

BLAST of CsGy5G009470 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 284.6 bits (727), Expect = 1.4e-75
Identity = 135/315 (42.86%), Postives = 204/315 (64.76%), Query Frame = 0

Query: 29  TVSLDGSIDHFKTITDAIASVPNNLNTR---FYIHVTPGTYHECLQIPPTKTFIALIGDN 88
           TV+ +G+  +F TIT+A+ S PN  +     F I+VT G Y E + I   K ++ +IGD 
Sbjct: 260 TVNQNGT-GNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAKNKRYLMMIGDG 319

Query: 89  ALTTIIVDDRSNARGFKTIDSATLTVNGNNFLAQSLTFKNSAGPQNGQAVAVLDEAHFTT 148
              T++  +R+   G+ T +SAT  V   NF+A ++TF+N+AGP+  QAVA+   A  + 
Sbjct: 320 INRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQAVAMRSSADLSI 379

Query: 149 YYKCRFLGFQDTLYVRGKPQFFKECDIYGSVDFIFGDGLVMFQDCNIYAREP--NRSITI 208
           +Y C F  +QDTLY     QF++ECDIYG+VDFIFG+  V+FQDCN+Y R+P  N+   I
Sbjct: 380 FYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYPRQPMQNQFNAI 439

Query: 209 TAQSKKQLHEESGFSFQNCTITISSEIAANKADVKIYLGRPWRQYSQVVFMESFLDKEVM 268
           TAQ +   ++ +G S  NCTI  + ++ ++   VK YLGRPW++YS+ VFM+S++D+ V 
Sbjct: 440 TAQGRTDPNQNTGISIHNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVFMQSYIDEVVE 499

Query: 269 PKGWLKWSG-VPLNNLFYGEFNNRGPGADVSKRIHCTGFHVLDKQSANQFTI-NFVNGSD 328
           P GW +W+G   L+ L+Y E+NN G G+  + R+   G+HV++   AN FT+ NF+ G  
Sbjct: 500 PVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINSTDANNFTVENFLLGDG 559

Query: 329 WLPETGVPFRSGLFS 337
           W+ ++GVP+ SGL S
Sbjct: 560 WMVQSGVPYISGLLS 573

BLAST of CsGy5G009470 vs. ExPASy Swiss-Prot
Match: O81415 (Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3702 GN=PME39 PE=2 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 9.2e-75
Identity = 136/318 (42.77%), Postives = 202/318 (63.52%), Query Frame = 0

Query: 21  IQSFTFNATVSLDGSIDHFKTITDAIASVPNNLNTRFYIHVTPGTYHECLQIPPTKTFIA 80
           +Q   +N +V++DG+  +F TI DA+ + PN   TRF I++  G Y E +++P  KT I 
Sbjct: 215 VQETNYNLSVAIDGT-GNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIM 274

Query: 81  LIGDNALTTIIVDDRSNARGFKTIDSATLTVNGNNFLAQSLTFKNSAGPQNGQAVAVLDE 140
            IGD    T+I  +RS   G+ T  + T+ V G  ++A+ ++F NSAGP   QAVA    
Sbjct: 275 FIGDGIGKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSG 334

Query: 141 AHFTTYYKCRFLGFQDTLYVRGKPQFFKECDIYGSVDFIFGDGLVMFQDCNIYAREPN-- 200
           +  + +Y+C F G+QDTLYV    QF++ECDIYG++DFIFG+  V+FQ+ ++YAR+PN  
Sbjct: 335 SDHSAFYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPG 394

Query: 201 RSITITAQSKKQLHEESGFSFQNCTITISSEIAANKADVKIYLGRPWRQYSQVVFMESFL 260
             I  TAQS+ Q  + +G S  NC I  + ++   K + K YLGRPWR+YS+ V ++SF+
Sbjct: 395 HKIAFTAQSRNQSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFI 454

Query: 261 DKEVMPKGWLKW-SGVPLNNLFYGEFNNRGPGADVSKRIHCTGFHVLDKQS-ANQFTIN- 320
           D  + P GWL+      L  L+YGE+ N GPGA+++KR+   GF  ++ Q+ A QFT+  
Sbjct: 455 DDLIHPAGWLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGP 514

Query: 321 FVNGSDWLPETGVPFRSG 334
           F++GS WL  TG+PF  G
Sbjct: 515 FIDGSTWLNSTGIPFSLG 531

BLAST of CsGy5G009470 vs. ExPASy Swiss-Prot
Match: Q94CB1 (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3702 GN=PME25 PE=2 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 7.8e-74
Identity = 131/308 (42.53%), Postives = 197/308 (63.96%), Query Frame = 0

Query: 37  DHFKTITDAIASVPNNLNTR---FYIHVTPGTYHECLQIPPTKTFIALIGDNALTTIIVD 96
           D+F TITDAIA+ PNN       F I+   G Y E + +P  K  + L+GD    TII  
Sbjct: 311 DNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTIITG 370

Query: 97  DRSNARGFKTIDSATLTVNGNNFLAQSLTFKNSAGPQNGQAVAVLDEAHFTTYYKCRFLG 156
           + +   G+ T + ++  V G  F+A  +TF+N+AGP+  QAVA+ + A  +++Y+C F G
Sbjct: 371 NHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFEG 430

Query: 157 FQDTLYVRGKPQFFKECDIYGSVDFIFGDGLVMFQDCNIYAREP--NRSITITAQSKKQL 216
           +QDTLYV    QF++ECDIYG+VDFIFG+   +FQ+CNIYAR+P   +   ITA  +   
Sbjct: 431 YQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLDP 490

Query: 217 HEESGFSFQNCTITISSEIAANKADVKIYLGRPWRQYSQVVFMESFLDKEVMPKGWLKWS 276
           ++ +G S  NCTI  + ++AA       +LGRPW+ YS+ VFM+S++   V P GWL+W+
Sbjct: 491 NQNTGISIINCTIKAAPDLAAEPKSAMTFLGRPWKPYSRTVFMQSYISDIVQPVGWLEWN 550

Query: 277 G-VPLNNLFYGEFNNRGPGADVSKRIHCTGFHVLDKQSANQFTI-NFVNGSDWLPETGVP 336
           G + L+ ++YGE++N GPGA+ ++R+   G+++L+   A  FT+ NF  G  WLP+T +P
Sbjct: 551 GTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWLPQTDIP 610

Query: 337 FRSGLFSE 338
           F  GL S+
Sbjct: 611 FYGGLLSK 618

BLAST of CsGy5G009470 vs. NCBI nr
Match: KAE8648077.1 (hypothetical protein Csa_018832 [Cucumis sativus])

HSP 1 Score: 691 bits (1782), Expect = 6.01e-251
Identity = 337/337 (100.00%), Postives = 337/337 (100.00%), Query Frame = 0

Query: 1   MKNLKVETIILLFFFNLLLYIQSFTFNATVSLDGSIDHFKTITDAIASVPNNLNTRFYIH 60
           MKNLKVETIILLFFFNLLLYIQSFTFNATVSLDGSIDHFKTITDAIASVPNNLNTRFYIH
Sbjct: 1   MKNLKVETIILLFFFNLLLYIQSFTFNATVSLDGSIDHFKTITDAIASVPNNLNTRFYIH 60

Query: 61  VTPGTYHECLQIPPTKTFIALIGDNALTTIIVDDRSNARGFKTIDSATLTVNGNNFLAQS 120
           VTPGTYHECLQIPPTKTFIALIGDNALTTIIVDDRSNARGFKTIDSATLTVNGNNFLAQS
Sbjct: 61  VTPGTYHECLQIPPTKTFIALIGDNALTTIIVDDRSNARGFKTIDSATLTVNGNNFLAQS 120

Query: 121 LTFKNSAGPQNGQAVAVLDEAHFTTYYKCRFLGFQDTLYVRGKPQFFKECDIYGSVDFIF 180
           LTFKNSAGPQNGQAVAVLDEAHFTTYYKCRFLGFQDTLYVRGKPQFFKECDIYGSVDFIF
Sbjct: 121 LTFKNSAGPQNGQAVAVLDEAHFTTYYKCRFLGFQDTLYVRGKPQFFKECDIYGSVDFIF 180

Query: 181 GDGLVMFQDCNIYAREPNRSITITAQSKKQLHEESGFSFQNCTITISSEIAANKADVKIY 240
           GDGLVMFQDCNIYAREPNRSITITAQSKKQLHEESGFSFQNCTITISSEIAANKADVKIY
Sbjct: 181 GDGLVMFQDCNIYAREPNRSITITAQSKKQLHEESGFSFQNCTITISSEIAANKADVKIY 240

Query: 241 LGRPWRQYSQVVFMESFLDKEVMPKGWLKWSGVPLNNLFYGEFNNRGPGADVSKRIHCTG 300
           LGRPWRQYSQVVFMESFLDKEVMPKGWLKWSGVPLNNLFYGEFNNRGPGADVSKRIHCTG
Sbjct: 241 LGRPWRQYSQVVFMESFLDKEVMPKGWLKWSGVPLNNLFYGEFNNRGPGADVSKRIHCTG 300

Query: 301 FHVLDKQSANQFTINFVNGSDWLPETGVPFRSGLFSE 337
           FHVLDKQSANQFTINFVNGSDWLPETGVPFRSGLFSE
Sbjct: 301 FHVLDKQSANQFTINFVNGSDWLPETGVPFRSGLFSE 337

BLAST of CsGy5G009470 vs. NCBI nr
Match: XP_031741287.1 (probable pectinesterase 56 [Cucumis sativus] >XP_031741292.1 probable pectinesterase 56 [Cucumis sativus])

HSP 1 Score: 637 bits (1644), Expect = 6.77e-229
Identity = 306/311 (98.39%), Postives = 309/311 (99.36%), Query Frame = 0

Query: 27  NATVSLDGSIDHFKTITDAIASVPNNLNTRFYIHVTPGTYHECLQIPPTKTFIALIGDNA 86
           NA ++ +GSIDHFKTITDAIASVPNNLNTRFYIHVTPGTYHECLQIPPTKTFIALIGDNA
Sbjct: 90  NALLTANGSIDHFKTITDAIASVPNNLNTRFYIHVTPGTYHECLQIPPTKTFIALIGDNA 149

Query: 87  LTTIIVDDRSNARGFKTIDSATLTVNGNNFLAQSLTFKNSAGPQNGQAVAVLDEAHFTTY 146
           LTTIIVDDRSNARGFKTIDSATLTVNGNNFLAQSLTFKNSAGPQNGQAVAVLDEAHFTTY
Sbjct: 150 LTTIIVDDRSNARGFKTIDSATLTVNGNNFLAQSLTFKNSAGPQNGQAVAVLDEAHFTTY 209

Query: 147 YKCRFLGFQDTLYVRGKPQFFKECDIYGSVDFIFGDGLVMFQDCNIYAREPNRSITITAQ 206
           YKCRFLGFQDTLYVRGKPQFFKECDIYGSVDFIFGDGLVMFQDCNIYAREPNRSITITAQ
Sbjct: 210 YKCRFLGFQDTLYVRGKPQFFKECDIYGSVDFIFGDGLVMFQDCNIYAREPNRSITITAQ 269

Query: 207 SKKQLHEESGFSFQNCTITISSEIAANKADVKIYLGRPWRQYSQVVFMESFLDKEVMPKG 266
           SKKQLHEESGFSFQNCTITISSEIAANKADVKIYLGRPWRQYSQVVFMESFLDKEVMPKG
Sbjct: 270 SKKQLHEESGFSFQNCTITISSEIAANKADVKIYLGRPWRQYSQVVFMESFLDKEVMPKG 329

Query: 267 WLKWSGVPLNNLFYGEFNNRGPGADVSKRIHCTGFHVLDKQSANQFTINFVNGSDWLPET 326
           WLKWSGVPLNNLFYGEFNNRGPGADVSKRIHCTGFHVLDKQSANQFTINFVNGSDWLPET
Sbjct: 330 WLKWSGVPLNNLFYGEFNNRGPGADVSKRIHCTGFHVLDKQSANQFTINFVNGSDWLPET 389

Query: 327 GVPFRSGLFSE 337
           GVPFRSGLFSE
Sbjct: 390 GVPFRSGLFSE 400

BLAST of CsGy5G009470 vs. NCBI nr
Match: KAA0055288.1 (putative pectinesterase/pectinesterase inhibitor 41 [Cucumis melo var. makuwa] >TYJ99211.1 putative pectinesterase/pectinesterase inhibitor 41 [Cucumis melo var. makuwa])

HSP 1 Score: 564 bits (1453), Expect = 7.97e-201
Identity = 275/337 (81.60%), Postives = 305/337 (90.50%), Query Frame = 0

Query: 1   MKNLKVETIILLFFFNLLLYIQSFTFNATVSLDGSIDHFKTITDAIASVPNNLNTRFYIH 60
           M+NLK   I ++ FFNL+ +I SFTFNATVSLDGS++HFKTI  AIA+ P+N NTRFYI 
Sbjct: 1   MENLKAVVITVMCFFNLVPFIHSFTFNATVSLDGSMNHFKTIAAAIAAAPDNSNTRFYIR 60

Query: 61  VTPGTYHECLQIPPTKTFIALIGDNALTTIIVDDRSNARGFKTIDSATLTVNGNNFLAQS 120
           VTPGTYHE LQIPPTKTFIALIGDNALTTIIVDDRSNARGFKT DSATLTVNGNNFLA+S
Sbjct: 61  VTPGTYHERLQIPPTKTFIALIGDNALTTIIVDDRSNARGFKTSDSATLTVNGNNFLAKS 120

Query: 121 LTFKNSAGPQNGQAVAVLDEAHFTTYYKCRFLGFQDTLYVRGKPQFFKECDIYGSVDFIF 180
           LTF+NSAGPQNGQAVAVLD+AHFT Y+KC+FLGFQDTLYV+G  QFFKEC+IYGSVDFIF
Sbjct: 121 LTFENSAGPQNGQAVAVLDQAHFTAYHKCKFLGFQDTLYVKGT-QFFKECEIYGSVDFIF 180

Query: 181 GDGLVMFQDCNIYAREPNRSITITAQSKKQLHEESGFSFQNCTITISSEIAANKADVKIY 240
           G+GLVMFQDCNIYAREPNR IT+TAQSK+Q  E SGFSFQNCT+T+SSEIA NKA+VKIY
Sbjct: 181 GNGLVMFQDCNIYAREPNRPITVTAQSKEQPKEVSGFSFQNCTVTVSSEIAVNKANVKIY 240

Query: 241 LGRPWRQYSQVVFMESFLDKEVMPKGWLKWSGVPLNNLFYGEFNNRGPGADVSKRIHCTG 300
           LGRPWRQYS+VVFMESFLD EV+P+GW +W GVP+NNLFY EFNNRGPGADVSKR+H TG
Sbjct: 241 LGRPWRQYSKVVFMESFLDDEVVPEGWSEWPGVPVNNLFYAEFNNRGPGADVSKRVHWTG 300

Query: 301 FHVLDKQSANQFTI-NFVNGSDWLPETGVPFRSGLFS 336
           F VLDKQSA QFT+ NFVNGS WLPETG+PFR GLFS
Sbjct: 301 FRVLDKQSAYQFTVDNFVNGSKWLPETGIPFRDGLFS 336

BLAST of CsGy5G009470 vs. NCBI nr
Match: KAA0055287.1 (putative pectinesterase/pectinesterase inhibitor 41 [Cucumis melo var. makuwa] >TYJ99210.1 putative pectinesterase/pectinesterase inhibitor 41 [Cucumis melo var. makuwa])

HSP 1 Score: 557 bits (1436), Expect = 3.21e-198
Identity = 269/337 (79.82%), Postives = 302/337 (89.61%), Query Frame = 0

Query: 1   MKNLKVETIILLFFFNLLLYIQSFTFNATVSLDGSIDHFKTITDAIASVPNNLNTRFYIH 60
           M+ LK   I ++ FFNL+ +I SFTFNATVSLDGS++ FKTI  AIA+ P+N NTRFYI 
Sbjct: 1   MEILKAVVITVMCFFNLVPFIHSFTFNATVSLDGSMNDFKTIAAAIAAAPDNSNTRFYIR 60

Query: 61  VTPGTYHECLQIPPTKTFIALIGDNALTTIIVDDRSNARGFKTIDSATLTVNGNNFLAQS 120
           VTPGTYHE LQIPPTKTFIALIGDNALTTIIVDDRSNARGFKT DSATLTVNG+NF+A+S
Sbjct: 61  VTPGTYHERLQIPPTKTFIALIGDNALTTIIVDDRSNARGFKTSDSATLTVNGDNFIAKS 120

Query: 121 LTFKNSAGPQNGQAVAVLDEAHFTTYYKCRFLGFQDTLYVRGKPQFFKECDIYGSVDFIF 180
           LTF+NSAGPQNGQAVAVLD+A FT Y+KCRFLGFQDTLYV GK  FFKECD+YG+VDFIF
Sbjct: 121 LTFENSAGPQNGQAVAVLDQARFTAYHKCRFLGFQDTLYVGGKSHFFKECDVYGTVDFIF 180

Query: 181 GDGLVMFQDCNIYAREPNRSITITAQSKKQLHEESGFSFQNCTITISSEIAANKADVKIY 240
           G+GL MFQDCNIYAREPNR IT+TAQSK+QLHEESGFSFQNCT+T+SSEI A+KA+VKIY
Sbjct: 181 GNGLTMFQDCNIYAREPNRPITVTAQSKEQLHEESGFSFQNCTVTVSSEIVASKANVKIY 240

Query: 241 LGRPWRQYSQVVFMESFLDKEVMPKGWLKWSGVPLNNLFYGEFNNRGPGADVSKRIHCTG 300
           L RPWRQYS+VVFMESFLD+EV+P+GW +W GVP NNLFYGEFNNRGPGADVSKR+H  G
Sbjct: 241 LDRPWRQYSRVVFMESFLDEEVLPEGWSEWPGVPTNNLFYGEFNNRGPGADVSKRVHWIG 300

Query: 301 FHVLDKQSANQFTI-NFVNGSDWLPETGVPFRSGLFS 336
           FH+L+KQ ANQFTI NFVNGS WLPETG+PFR G FS
Sbjct: 301 FHLLNKQLANQFTIDNFVNGSYWLPETGIPFRRGFFS 337

BLAST of CsGy5G009470 vs. NCBI nr
Match: XP_004152582.2 (pectinesterase 2 [Cucumis sativus] >KGN64355.1 hypothetical protein Csa_013871 [Cucumis sativus])

HSP 1 Score: 481 bits (1237), Expect = 6.09e-168
Identity = 233/324 (71.91%), Postives = 272/324 (83.95%), Query Frame = 0

Query: 14  FFNLLLYIQSFTFNATVSLDGSIDHFKTITDAIASVPNNLNTRFYIHVTPGTYHECLQIP 73
           FFNL+L I S +FN TVSLDGS  +FK I+DAIA+ PNN NTRFYIHV PGTYHE LQIP
Sbjct: 14  FFNLMLLIHSLSFNLTVSLDGS-GNFKRISDAIAAAPNNSNTRFYIHVAPGTYHETLQIP 73

Query: 74  PTKTFIALIGDNALTTIIVDDRSNARGFKTIDSATLTVNGNNFLAQSLTFKNSAGPQNGQ 133
             K FIAL+GD+  TTII+D+RSN  GFKT +SATLTV G+NF+A+SLTF+NSAGPQN Q
Sbjct: 74  RLKKFIALVGDDPFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAESLTFENSAGPQNNQ 133

Query: 134 AVAVLDEAHFTTYYKCRFLGFQDTLYVRGKPQFFKECDIYGSVDFIFGDGLVMFQDCNIY 193
           AVAV D+A+ T YYKCRFL FQDTLYV GKPQFFKE DIYGSVDFI G G VMFQDCNIY
Sbjct: 134 AVAVFDKANHTAYYKCRFLSFQDTLYVNGKPQFFKESDIYGSVDFICGYGQVMFQDCNIY 193

Query: 194 AREPNRSITITAQSKKQLHEESGFSFQNCTITISSEIAANKADVKIYLGRPWRQYSQVVF 253
           AR P  SIT+TAQSK  L   SGFSFQNCT+T+S EI++NK +VK++LGRPW+QYS+VVF
Sbjct: 194 ARMPINSITVTAQSKYILRSVSGFSFQNCTVTVSREISSNKQNVKVFLGRPWKQYSKVVF 253

Query: 254 MESFLDKEVMPKGWLKWSGVPLNNLFYGEFNNRGPGADVSKRIHCTGFHVLDKQSANQFT 313
           MESFLD  V  +GW++W GVP+NNLFYGEFNN GPGADVSKR++ T +H+LDK+SA +FT
Sbjct: 254 MESFLDDVVASEGWVEWIGVPVNNLFYGEFNNCGPGADVSKRVNWTSYHLLDKESALRFT 313

Query: 314 I-NFVNGSDWLPETGVPFRSGLFS 336
           + NFVNGS+WLPETG+PFR GL S
Sbjct: 314 VDNFVNGSEWLPETGIPFRRGLHS 336

BLAST of CsGy5G009470 vs. ExPASy TrEMBL
Match: A0A5D3BKF8 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004210 PE=4 SV=1)

HSP 1 Score: 564 bits (1453), Expect = 3.86e-201
Identity = 275/337 (81.60%), Postives = 305/337 (90.50%), Query Frame = 0

Query: 1   MKNLKVETIILLFFFNLLLYIQSFTFNATVSLDGSIDHFKTITDAIASVPNNLNTRFYIH 60
           M+NLK   I ++ FFNL+ +I SFTFNATVSLDGS++HFKTI  AIA+ P+N NTRFYI 
Sbjct: 1   MENLKAVVITVMCFFNLVPFIHSFTFNATVSLDGSMNHFKTIAAAIAAAPDNSNTRFYIR 60

Query: 61  VTPGTYHECLQIPPTKTFIALIGDNALTTIIVDDRSNARGFKTIDSATLTVNGNNFLAQS 120
           VTPGTYHE LQIPPTKTFIALIGDNALTTIIVDDRSNARGFKT DSATLTVNGNNFLA+S
Sbjct: 61  VTPGTYHERLQIPPTKTFIALIGDNALTTIIVDDRSNARGFKTSDSATLTVNGNNFLAKS 120

Query: 121 LTFKNSAGPQNGQAVAVLDEAHFTTYYKCRFLGFQDTLYVRGKPQFFKECDIYGSVDFIF 180
           LTF+NSAGPQNGQAVAVLD+AHFT Y+KC+FLGFQDTLYV+G  QFFKEC+IYGSVDFIF
Sbjct: 121 LTFENSAGPQNGQAVAVLDQAHFTAYHKCKFLGFQDTLYVKGT-QFFKECEIYGSVDFIF 180

Query: 181 GDGLVMFQDCNIYAREPNRSITITAQSKKQLHEESGFSFQNCTITISSEIAANKADVKIY 240
           G+GLVMFQDCNIYAREPNR IT+TAQSK+Q  E SGFSFQNCT+T+SSEIA NKA+VKIY
Sbjct: 181 GNGLVMFQDCNIYAREPNRPITVTAQSKEQPKEVSGFSFQNCTVTVSSEIAVNKANVKIY 240

Query: 241 LGRPWRQYSQVVFMESFLDKEVMPKGWLKWSGVPLNNLFYGEFNNRGPGADVSKRIHCTG 300
           LGRPWRQYS+VVFMESFLD EV+P+GW +W GVP+NNLFY EFNNRGPGADVSKR+H TG
Sbjct: 241 LGRPWRQYSKVVFMESFLDDEVVPEGWSEWPGVPVNNLFYAEFNNRGPGADVSKRVHWTG 300

Query: 301 FHVLDKQSANQFTI-NFVNGSDWLPETGVPFRSGLFS 336
           F VLDKQSA QFT+ NFVNGS WLPETG+PFR GLFS
Sbjct: 301 FRVLDKQSAYQFTVDNFVNGSKWLPETGIPFRDGLFS 336

BLAST of CsGy5G009470 vs. ExPASy TrEMBL
Match: A0A5D3BHF7 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004200 PE=4 SV=1)

HSP 1 Score: 557 bits (1436), Expect = 1.55e-198
Identity = 269/337 (79.82%), Postives = 302/337 (89.61%), Query Frame = 0

Query: 1   MKNLKVETIILLFFFNLLLYIQSFTFNATVSLDGSIDHFKTITDAIASVPNNLNTRFYIH 60
           M+ LK   I ++ FFNL+ +I SFTFNATVSLDGS++ FKTI  AIA+ P+N NTRFYI 
Sbjct: 1   MEILKAVVITVMCFFNLVPFIHSFTFNATVSLDGSMNDFKTIAAAIAAAPDNSNTRFYIR 60

Query: 61  VTPGTYHECLQIPPTKTFIALIGDNALTTIIVDDRSNARGFKTIDSATLTVNGNNFLAQS 120
           VTPGTYHE LQIPPTKTFIALIGDNALTTIIVDDRSNARGFKT DSATLTVNG+NF+A+S
Sbjct: 61  VTPGTYHERLQIPPTKTFIALIGDNALTTIIVDDRSNARGFKTSDSATLTVNGDNFIAKS 120

Query: 121 LTFKNSAGPQNGQAVAVLDEAHFTTYYKCRFLGFQDTLYVRGKPQFFKECDIYGSVDFIF 180
           LTF+NSAGPQNGQAVAVLD+A FT Y+KCRFLGFQDTLYV GK  FFKECD+YG+VDFIF
Sbjct: 121 LTFENSAGPQNGQAVAVLDQARFTAYHKCRFLGFQDTLYVGGKSHFFKECDVYGTVDFIF 180

Query: 181 GDGLVMFQDCNIYAREPNRSITITAQSKKQLHEESGFSFQNCTITISSEIAANKADVKIY 240
           G+GL MFQDCNIYAREPNR IT+TAQSK+QLHEESGFSFQNCT+T+SSEI A+KA+VKIY
Sbjct: 181 GNGLTMFQDCNIYAREPNRPITVTAQSKEQLHEESGFSFQNCTVTVSSEIVASKANVKIY 240

Query: 241 LGRPWRQYSQVVFMESFLDKEVMPKGWLKWSGVPLNNLFYGEFNNRGPGADVSKRIHCTG 300
           L RPWRQYS+VVFMESFLD+EV+P+GW +W GVP NNLFYGEFNNRGPGADVSKR+H  G
Sbjct: 241 LDRPWRQYSRVVFMESFLDEEVLPEGWSEWPGVPTNNLFYGEFNNRGPGADVSKRVHWIG 300

Query: 301 FHVLDKQSANQFTI-NFVNGSDWLPETGVPFRSGLFS 336
           FH+L+KQ ANQFTI NFVNGS WLPETG+PFR G FS
Sbjct: 301 FHLLNKQLANQFTIDNFVNGSYWLPETGIPFRRGFFS 337

BLAST of CsGy5G009470 vs. ExPASy TrEMBL
Match: A0A0A0LR14 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G049450 PE=4 SV=1)

HSP 1 Score: 481 bits (1237), Expect = 2.95e-168
Identity = 233/324 (71.91%), Postives = 272/324 (83.95%), Query Frame = 0

Query: 14  FFNLLLYIQSFTFNATVSLDGSIDHFKTITDAIASVPNNLNTRFYIHVTPGTYHECLQIP 73
           FFNL+L I S +FN TVSLDGS  +FK I+DAIA+ PNN NTRFYIHV PGTYHE LQIP
Sbjct: 14  FFNLMLLIHSLSFNLTVSLDGS-GNFKRISDAIAAAPNNSNTRFYIHVAPGTYHETLQIP 73

Query: 74  PTKTFIALIGDNALTTIIVDDRSNARGFKTIDSATLTVNGNNFLAQSLTFKNSAGPQNGQ 133
             K FIAL+GD+  TTII+D+RSN  GFKT +SATLTV G+NF+A+SLTF+NSAGPQN Q
Sbjct: 74  RLKKFIALVGDDPFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAESLTFENSAGPQNNQ 133

Query: 134 AVAVLDEAHFTTYYKCRFLGFQDTLYVRGKPQFFKECDIYGSVDFIFGDGLVMFQDCNIY 193
           AVAV D+A+ T YYKCRFL FQDTLYV GKPQFFKE DIYGSVDFI G G VMFQDCNIY
Sbjct: 134 AVAVFDKANHTAYYKCRFLSFQDTLYVNGKPQFFKESDIYGSVDFICGYGQVMFQDCNIY 193

Query: 194 AREPNRSITITAQSKKQLHEESGFSFQNCTITISSEIAANKADVKIYLGRPWRQYSQVVF 253
           AR P  SIT+TAQSK  L   SGFSFQNCT+T+S EI++NK +VK++LGRPW+QYS+VVF
Sbjct: 194 ARMPINSITVTAQSKYILRSVSGFSFQNCTVTVSREISSNKQNVKVFLGRPWKQYSKVVF 253

Query: 254 MESFLDKEVMPKGWLKWSGVPLNNLFYGEFNNRGPGADVSKRIHCTGFHVLDKQSANQFT 313
           MESFLD  V  +GW++W GVP+NNLFYGEFNN GPGADVSKR++ T +H+LDK+SA +FT
Sbjct: 254 MESFLDDVVASEGWVEWIGVPVNNLFYGEFNNCGPGADVSKRVNWTSYHLLDKESALRFT 313

Query: 314 I-NFVNGSDWLPETGVPFRSGLFS 336
           + NFVNGS+WLPETG+PFR GL S
Sbjct: 314 VDNFVNGSEWLPETGIPFRRGLHS 336

BLAST of CsGy5G009470 vs. ExPASy TrEMBL
Match: A0A5A7ULX7 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G001290 PE=4 SV=1)

HSP 1 Score: 475 bits (1223), Expect = 2.16e-166
Identity = 227/320 (70.94%), Postives = 269/320 (84.06%), Query Frame = 0

Query: 18  LLYIQSFTFNATVSLDGSIDHFKTITDAIASVPNNLNTRFYIHVTPGTYHECLQIPPTKT 77
           +L++ S +FN TVSLDGS  +FK I+DAI + PNN NTRFYIHV PGTYHE +QIP  K 
Sbjct: 1   MLFVHSISFNLTVSLDGS-GNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKK 60

Query: 78  FIALIGDNALTTIIVDDRSNARGFKTIDSATLTVNGNNFLAQSLTFKNSAGPQNGQAVAV 137
           FIALIGD+A TTII+D+RSN  GFKT +SATLTV G+NF+A+SLTF+NSAGPQNGQAVAV
Sbjct: 61  FIALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGDNFMAKSLTFENSAGPQNGQAVAV 120

Query: 138 LDEAHFTTYYKCRFLGFQDTLYVRGKPQFFKECDIYGSVDFIFGDGLVMFQDCNIYAREP 197
            D+A  T YYKCRFLGFQDTLYV GK QFFKE +IYGSVDFIFG G VMFQDCNIYAR P
Sbjct: 121 FDKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGRVMFQDCNIYARMP 180

Query: 198 NRSITITAQSKKQLHEESGFSFQNCTITISSEIAANKADVKIYLGRPWRQYSQVVFMESF 257
           + SIT+TAQSK  L   SGFSFQNCT+T+S EI++NK +VK++LGRPWRQYS VVFMESF
Sbjct: 181 SNSITVTAQSKDFLRSISGFSFQNCTVTVSPEISSNKQNVKVFLGRPWRQYSNVVFMESF 240

Query: 258 LDKEVMPKGWLKWSGVPLNNLFYGEFNNRGPGADVSKRIHCTGFHVLDKQSANQFTI-NF 317
           LD  V  +GW++W GVP+NNLFYGEFNN GPGADVSKR++ TG+H+LDK+SA  FT+ NF
Sbjct: 241 LDDVVASEGWMEWKGVPVNNLFYGEFNNFGPGADVSKRVNWTGYHLLDKKSAIGFTVDNF 300

Query: 318 VNGSDWLPETGVPFRSGLFS 336
           ++GS+WL ETG+PFR GL +
Sbjct: 301 IDGSEWLAETGIPFRRGLLT 319

BLAST of CsGy5G009470 vs. ExPASy TrEMBL
Match: A0A5D3BM28 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004180 PE=4 SV=1)

HSP 1 Score: 465 bits (1197), Expect = 1.95e-162
Identity = 224/320 (70.00%), Postives = 265/320 (82.81%), Query Frame = 0

Query: 18  LLYIQSFTFNATVSLDGSIDHFKTITDAIASVPNNLNTRFYIHVTPGTYHECLQIPPTKT 77
           +L++ S +FN TVSLDGS  +FK I+DAI + PNN NTRFYIHV PGTYHE +QIP  K 
Sbjct: 1   MLFVHSISFNLTVSLDGS-GNFKRISDAIVAAPNNSNTRFYIHVAPGTYHEAIQIPKAKK 60

Query: 78  FIALIGDNALTTIIVDDRSNARGFKTIDSATLTVNGNNFLAQSLTFKNSAGPQNGQAVAV 137
           FIALIGD+A TTII+D+RSN  GFKT +SATLTV GNNF+A+SLTF+NSAGPQNGQAVAV
Sbjct: 61  FIALIGDDAFTTIIIDNRSNGTGFKTNNSATLTVQGNNFMAKSLTFENSAGPQNGQAVAV 120

Query: 138 LDEAHFTTYYKCRFLGFQDTLYVRGKPQFFKECDIYGSVDFIFGDGLVMFQDCNIYAREP 197
            D+A  T YYKCRFLGFQDTLYV GK QFFKE +IYGSVDFIFG G VMFQDCNIYAR P
Sbjct: 121 FDKADHTAYYKCRFLGFQDTLYVNGKYQFFKESNIYGSVDFIFGHGQVMFQDCNIYARMP 180

Query: 198 NRSITITAQSKKQLHEESGFSFQNCTITISSEIAANKADVKIYLGRPWRQYSQVVFMESF 257
           + SIT+TAQSK  L   SGFSFQN T+T+  EI++NK +VK++LGR WRQYS VVFMESF
Sbjct: 181 SNSITVTAQSKDFLRSISGFSFQNYTVTVLPEISSNKQNVKVFLGRLWRQYSNVVFMESF 240

Query: 258 LDKEVMPKGWLKWSGVPLNNLFYGEFNNRGPGADVSKRIHCTGFHVLDKQSANQFTI-NF 317
           LD  V  +GW++W GVP+NNLFYGEFNN GPGADVSKR++ TG+H+L+K+SA +FT+ NF
Sbjct: 241 LDDVVASEGWMEWKGVPINNLFYGEFNNYGPGADVSKRVNWTGYHILNKESAFRFTVDNF 300

Query: 318 VNGSDWLPETGVPFRSGLFS 336
           +N S WL ETG+PFR GL +
Sbjct: 301 INRSKWLHETGIPFRHGLLT 319

BLAST of CsGy5G009470 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 285.0 bits (728), Expect = 7.7e-77
Identity = 137/316 (43.35%), Postives = 206/316 (65.19%), Query Frame = 0

Query: 29  TVSLDGSIDHFKTITDAIASVPNNLNTR---FYIHVTPGTYHECLQIPPTKTFIALIGDN 88
           TVS DG+  +F  IT A+A+ PNN +     F I+VT G Y E + I   K ++ +IGD 
Sbjct: 266 TVSQDGT-GNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEEYISIAKNKRYMMMIGDG 325

Query: 89  ALTTIIVDDRSNARGFKTIDSATLTVNGNNFLAQSLTFKNSAGPQNGQAVAVLDEAHFTT 148
              T++  +RS   G+ T +SAT  V   NF+A ++TF+N+AGP+  QAVA+   A F+ 
Sbjct: 326 INQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRNTAGPEKHQAVALRSGADFSI 385

Query: 149 YYKCRFLGFQDTLYVRGKPQFFKECDIYGSVDFIFGDGLVMFQDCNIYARE--PNRSITI 208
           +Y C F  +QDTLY     QF++ECD+YG+VDFIFG+  V+FQ+CN+Y R+  PN+   I
Sbjct: 386 FYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVFQNCNLYPRKPMPNQFNAI 445

Query: 209 TAQSKKQLHEESGFSFQNCTITISSEIAANKADVKIYLGRPWRQYSQVVFMESFLDKEVM 268
           TAQ +   ++ +G S QNCTI  + ++ ++   VK YLGRPW++YS+ V+M+S++D  V 
Sbjct: 446 TAQGRSDPNQNTGTSIQNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVYMQSYIDGFVE 505

Query: 269 PKGWLKWSG-VPLNNLFYGEFNNRGPGADVSKRIHCTGFHVLDKQSANQFTIN--FVNGS 328
           P GW +W+G   L+ L+Y E+NN GPG++ + R+   G+HV++   A  FT+   F+  +
Sbjct: 506 PVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGYHVINSTDAANFTVTGLFIE-A 565

Query: 329 DWLPETGVPFRSGLFS 337
           DW+ +TGVP+ SGL S
Sbjct: 566 DWIWKTGVPYTSGLIS 579

BLAST of CsGy5G009470 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 284.6 bits (727), Expect = 1.0e-76
Identity = 135/315 (42.86%), Postives = 204/315 (64.76%), Query Frame = 0

Query: 29  TVSLDGSIDHFKTITDAIASVPNNLNTR---FYIHVTPGTYHECLQIPPTKTFIALIGDN 88
           TV+ +G+  +F TIT+A+ S PN  +     F I+VT G Y E + I   K ++ +IGD 
Sbjct: 260 TVNQNGT-GNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAKNKRYLMMIGDG 319

Query: 89  ALTTIIVDDRSNARGFKTIDSATLTVNGNNFLAQSLTFKNSAGPQNGQAVAVLDEAHFTT 148
              T++  +R+   G+ T +SAT  V   NF+A ++TF+N+AGP+  QAVA+   A  + 
Sbjct: 320 INRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQAVAMRSSADLSI 379

Query: 149 YYKCRFLGFQDTLYVRGKPQFFKECDIYGSVDFIFGDGLVMFQDCNIYAREP--NRSITI 208
           +Y C F  +QDTLY     QF++ECDIYG+VDFIFG+  V+FQDCN+Y R+P  N+   I
Sbjct: 380 FYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYPRQPMQNQFNAI 439

Query: 209 TAQSKKQLHEESGFSFQNCTITISSEIAANKADVKIYLGRPWRQYSQVVFMESFLDKEVM 268
           TAQ +   ++ +G S  NCTI  + ++ ++   VK YLGRPW++YS+ VFM+S++D+ V 
Sbjct: 440 TAQGRTDPNQNTGISIHNCTIKPADDLVSSNYTVKTYLGRPWKEYSRTVFMQSYIDEVVE 499

Query: 269 PKGWLKWSG-VPLNNLFYGEFNNRGPGADVSKRIHCTGFHVLDKQSANQFTI-NFVNGSD 328
           P GW +W+G   L+ L+Y E+NN G G+  + R+   G+HV++   AN FT+ NF+ G  
Sbjct: 500 PVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVINSTDANNFTVENFLLGDG 559

Query: 329 WLPETGVPFRSGLFS 337
           W+ ++GVP+ SGL S
Sbjct: 560 WMVQSGVPYISGLLS 573

BLAST of CsGy5G009470 vs. TAIR 10
Match: AT4G02300.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 282.0 bits (720), Expect = 6.5e-76
Identity = 136/318 (42.77%), Postives = 202/318 (63.52%), Query Frame = 0

Query: 21  IQSFTFNATVSLDGSIDHFKTITDAIASVPNNLNTRFYIHVTPGTYHECLQIPPTKTFIA 80
           +Q   +N +V++DG+  +F TI DA+ + PN   TRF I++  G Y E +++P  KT I 
Sbjct: 215 VQETNYNLSVAIDGT-GNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIM 274

Query: 81  LIGDNALTTIIVDDRSNARGFKTIDSATLTVNGNNFLAQSLTFKNSAGPQNGQAVAVLDE 140
            IGD    T+I  +RS   G+ T  + T+ V G  ++A+ ++F NSAGP   QAVA    
Sbjct: 275 FIGDGIGKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSG 334

Query: 141 AHFTTYYKCRFLGFQDTLYVRGKPQFFKECDIYGSVDFIFGDGLVMFQDCNIYAREPN-- 200
           +  + +Y+C F G+QDTLYV    QF++ECDIYG++DFIFG+  V+FQ+ ++YAR+PN  
Sbjct: 335 SDHSAFYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPG 394

Query: 201 RSITITAQSKKQLHEESGFSFQNCTITISSEIAANKADVKIYLGRPWRQYSQVVFMESFL 260
             I  TAQS+ Q  + +G S  NC I  + ++   K + K YLGRPWR+YS+ V ++SF+
Sbjct: 395 HKIAFTAQSRNQSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFI 454

Query: 261 DKEVMPKGWLKW-SGVPLNNLFYGEFNNRGPGADVSKRIHCTGFHVLDKQS-ANQFTIN- 320
           D  + P GWL+      L  L+YGE+ N GPGA+++KR+   GF  ++ Q+ A QFT+  
Sbjct: 455 DDLIHPAGWLEGKKDFALETLYYGEYMNEGPGANMAKRVTWPGFRRIENQTEATQFTVGP 514

Query: 321 FVNGSDWLPETGVPFRSG 334
           F++GS WL  TG+PF  G
Sbjct: 515 FIDGSTWLNSTGIPFSLG 531

BLAST of CsGy5G009470 vs. TAIR 10
Match: AT3G10720.2 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 278.9 bits (712), Expect = 5.5e-75
Identity = 131/308 (42.53%), Postives = 197/308 (63.96%), Query Frame = 0

Query: 37  DHFKTITDAIASVPNNLNTR---FYIHVTPGTYHECLQIPPTKTFIALIGDNALTTIIVD 96
           D+F TITDAIA+ PNN       F I+   G Y E + +P  K  + L+GD    TII  
Sbjct: 311 DNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTIITG 370

Query: 97  DRSNARGFKTIDSATLTVNGNNFLAQSLTFKNSAGPQNGQAVAVLDEAHFTTYYKCRFLG 156
           + +   G+ T + ++  V G  F+A  +TF+N+AGP+  QAVA+ + A  +++Y+C F G
Sbjct: 371 NHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFEG 430

Query: 157 FQDTLYVRGKPQFFKECDIYGSVDFIFGDGLVMFQDCNIYAREP--NRSITITAQSKKQL 216
           +QDTLYV    QF++ECDIYG+VDFIFG+   +FQ+CNIYAR+P   +   ITA  +   
Sbjct: 431 YQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLDP 490

Query: 217 HEESGFSFQNCTITISSEIAANKADVKIYLGRPWRQYSQVVFMESFLDKEVMPKGWLKWS 276
           ++ +G S  NCTI  + ++AA       +LGRPW+ YS+ VFM+S++   V P GWL+W+
Sbjct: 491 NQNTGISIINCTIKAAPDLAAEPKSAMTFLGRPWKPYSRTVFMQSYISDIVQPVGWLEWN 550

Query: 277 G-VPLNNLFYGEFNNRGPGADVSKRIHCTGFHVLDKQSANQFTI-NFVNGSDWLPETGVP 336
           G + L+ ++YGE++N GPGA+ ++R+   G+++L+   A  FT+ NF  G  WLP+T +P
Sbjct: 551 GTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYNFTMGDTWLPQTDIP 610

Query: 337 FRSGLFSE 338
           F  GL S+
Sbjct: 611 FYGGLLSK 618

BLAST of CsGy5G009470 vs. TAIR 10
Match: AT2G47550.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 276.9 bits (707), Expect = 2.1e-74
Identity = 127/306 (41.50%), Postives = 194/306 (63.40%), Query Frame = 0

Query: 38  HFKTITDAIASVPNNL---NTRFYIHVTPGTYHECLQIPPTKTFIALIGDNALTTIIVDD 97
           +F TI  AIA+ PN     N  F I+VT G Y E +++P  K ++ +IGD    T+I  +
Sbjct: 255 NFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTVITGN 314

Query: 98  RSNARGFKTIDSATLTVNGNNFLAQSLTFKNSAGPQNGQAVAVLDEAHFTTYYKCRFLGF 157
           RS   G+ T +SAT  ++G NF+  ++T +N+AGP  GQAVA+      + +Y C F  +
Sbjct: 315 RSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVFYSCSFEAY 374

Query: 158 QDTLYVRGKPQFFKECDIYGSVDFIFGDGLVMFQDCNIYAREPNR--SITITAQSKKQLH 217
           QDTLY     QF++ECD+YG+VDFIFG+  V+ Q+CN+Y R+P +  S  +TAQ +   +
Sbjct: 375 QDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVTAQGRTDPN 434

Query: 218 EESGFSFQNCTITISSEIAANKADVKIYLGRPWRQYSQVVFMESFLDKEVMPKGWLKWSG 277
           + +G +   CTI  + ++A +   VK YLGRPW++YS+ V M++++D  + P GW  WSG
Sbjct: 435 QNTGTAIHGCTIRPADDLATSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSGWNAWSG 494

Query: 278 -VPLNNLFYGEFNNRGPGADVSKRIHCTGFHVLDKQSANQFTI-NFVNGSDWLPETGVPF 337
              L+ L+Y E+NN GPG+D + R+   G+HV++   A+ FT+ NF+ G  W+ +TGVPF
Sbjct: 495 DFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINATDASNFTVTNFLVGEGWIGQTGVPF 554

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O048876.3e-7643.91Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1[more]
Q9SRX41.1e-7543.35Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=37... [more]
Q8RXK71.4e-7542.86Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3... [more]
O814159.2e-7542.77Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3... [more]
Q94CB17.8e-7442.53Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
KAE8648077.16.01e-251100.00hypothetical protein Csa_018832 [Cucumis sativus][more]
XP_031741287.16.77e-22998.39probable pectinesterase 56 [Cucumis sativus] >XP_031741292.1 probable pectineste... [more]
KAA0055288.17.97e-20181.60putative pectinesterase/pectinesterase inhibitor 41 [Cucumis melo var. makuwa] >... [more]
KAA0055287.13.21e-19879.82putative pectinesterase/pectinesterase inhibitor 41 [Cucumis melo var. makuwa] >... [more]
XP_004152582.26.09e-16871.91pectinesterase 2 [Cucumis sativus] >KGN64355.1 hypothetical protein Csa_013871 [... [more]
Match NameE-valueIdentityDescription
A0A5D3BKF83.86e-20181.60Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00421... [more]
A0A5D3BHF71.55e-19879.82Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00420... [more]
A0A0A0LR142.95e-16871.91Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G049450 PE=4 SV=1[more]
A0A5A7ULX72.16e-16670.94Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold80G001290... [more]
A0A5D3BM281.95e-16270.00Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00418... [more]
Match NameE-valueIdentityDescription
AT1G02810.17.7e-7743.35Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G02330.11.0e-7642.86Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G02300.16.5e-7642.77Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G10720.25.5e-7542.53Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G47550.12.1e-7441.50Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 22..334
e-value: 2.9E-105
score: 354.2
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 27..317
e-value: 8.4E-101
score: 336.6
NoneNo IPR availablePANTHERPTHR31707:SF205PECTINESTERASE/PECTINESTERASE INHIBITOR 22-RELATEDcoord: 21..333
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 21..333
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 172..181
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 25..332

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy5G009470.2CsGy5G009470.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity