CsGy5G005800 (gene) Cucumber (Gy14) v2.1

BLAST of CsGy5G005800 vs. ExPASy Swiss-Prot
Match: Q9FXW4 (Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica OX=3442 GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 9.5e-116
Identity = 233/501 (46.51%), Postives = 322/501 (64.27%), Query Frame = 0

Query: 11  MLIPSFSILLPSLLLLLPPIFIILKRIKSPSSKKLPLPPGPNPWPILGNLLQIGQNAHIS 70
           M + S +I+  S LL L   F IL+      S+   LPPGP P PI+GNLLQ+G   H  
Sbjct: 1   MEVLSIAIVSFSFLLFL---FFILR-----DSRPKNLPPGPRPSPIVGNLLQLGDKPHAE 60

Query: 71  ITQFANIYGPLISFKLGAQRLVVASSPAAATAVLKTQDHLLSARYIFQITPDRALHDQHS 130
             + A  YG L S KLG+Q +VVASSPAAA  +LKT D +LS RY+FQ    +  H ++S
Sbjct: 61  FAKLAQKYGELFSLKLGSQTVVVASSPAAAAEILKTHDKILSGRYVFQSFRVKE-HVENS 120

Query: 131 LVFSSECGDVWKKLRSICKVNLFTAKAIESQAILREKKMKELVEFVESKQGSVVEIKDFV 190
           +V+ SEC D WK LR +C+  LFT K IESQ+ +RE K +E+V+F+  K+G VV+I + V
Sbjct: 121 IVW-SECNDNWKLLRKVCRTELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVKIVEVV 180

Query: 191 FTSAFNILSNLIFSMDLLDFVG--DGFNGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQG 250
           F +  NI  NLIFS D+ D      G   +K    K++D+G + N AD++P++ + DL G
Sbjct: 181 FGTLVNIFGNLIFSKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFG 240

Query: 251 LIRKTVIYKNEVNSVWGILIKERREIRCEKGSAS-NDFLDVLIQSGFSDQQIDALIIELL 310
             R       ++  VWG+++KERR     KGS S NDF+DVL+ +G  DQ+I+AL++EL 
Sbjct: 241 QRRAVAEVLQQIYDVWGVMLKERRG---TKGSESKNDFVDVLLNAGLDDQKINALLMELF 300

Query: 311 FAATDTTTTTVEWAMSEILKDKDILNKAREEIKKVAKESPAIDESNISKLQYLWSCVKET 370
            A T+T+ +T+EWA++E+ K   +++K R E+  V  ++  + ES++  L YL + VKET
Sbjct: 301 GAGTETSASTIEWAITELTKKPLVVSKIRLELVNVVGDN-TVKESDLPHLPYLQAFVKET 360

Query: 371 LRLHPPVPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFKPERFDVG 430
           LRLHPP P LLPR A   C VM Y+IPK+  I VN W IGRDP  W+D   FKPERF   
Sbjct: 361 LRLHPPTPLLLPRRALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERF--- 420

Query: 431 CGNNMDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDL 490
             +++D+KG D+  +PFGGGRRICPGLP+A     LI+ATL+ NFEWSLP G   S+L +
Sbjct: 421 LSSDVDYKGNDFELIPFGGGRRICPGLPLASQFSNLIVATLVQNFEWSLPQGMSTSELSM 480

Query: 491 NGRLGVTLQKYETLKLIPKRR 509
           + + G+TLQK   L ++ K R
Sbjct: 481 DEKFGLTLQKDPPLLIVLKAR 484

BLAST of CsGy5G005800 vs. ExPASy Swiss-Prot
Match: Q9SP06 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum OX=3469 GN=CYP80B3 PE=1 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 1.6e-115
Identity = 212/465 (45.59%), Postives = 303/465 (65.16%), Query Frame = 0

Query: 47  LPPGPNPWPILGNLLQIGQNAHISITQFANIYGPLISFKLGAQRLVVASSPAAATAVLKT 106
           LPPGP PWPI+GNLLQ+G+  H    Q A  YG L S KLG++ +VVAS+P AA+ +LKT
Sbjct: 23  LPPGPKPWPIVGNLLQLGEKPHSQFAQLAETYGDLFSLKLGSETVVVASTPLAASEILKT 82

Query: 107 QDHLLSARYIFQITPDRALHDQHSLVFSSECGDVWKKLRSICKVNLFTAKAIESQAILRE 166
            D +LS RY+FQ    +  H ++S+V+ SEC + WKKLR +C+  LFT K IESQA +RE
Sbjct: 83  HDRVLSGRYVFQSFRVKE-HVENSIVW-SECNETWKKLRKVCRTELFTQKMIESQAEVRE 142

Query: 167 KKMKELVEFVESKQGSVVEIKDFVFTSAFNILSNLIFSMDLLDFVGDGFNG---IKGPFK 226
            K  E+VE+++   G+ V+I + VF +  NI  NLIFS ++   +GD  +G   +K    
Sbjct: 143 SKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFK-LGDESSGSVEMKEHLW 202

Query: 227 KMMDLGLTPNLADFYPLLRRFDLQGLIRKTVIYKNEVNSVWGILIKERREIRCEKGSASN 286
           +M++LG + N AD++P L +FDL G  +        + SVWG ++KER+  +    S  N
Sbjct: 203 RMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVWGAMLKERKIAKQHNNSKKN 262

Query: 287 DFLDVLIQSGFSDQQIDALIIELLFAATDTTTTTVEWAMSEILKDKDILNKAREEIKKVA 346
           DF+++L+ SG  DQQI+AL++E+  A T+T+ +T+EWA+SE+ K+  +    R E+  V 
Sbjct: 263 DFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPQVTANMRLELLSVV 322

Query: 347 KESPAIDESNISKLQYLWSCVKETLRLHPPVPFLLPRLAPMDCEVMGYSIPKDSMIFVNV 406
            + P + ES+I  + YL + VKETLRLHP  P LLPR A   C+V+ Y+IPK+  I VN 
Sbjct: 323 GKRP-VKESDIPNMPYLQAFVKETLRLHPATPLLLPRRALETCKVLNYTIPKECQIMVNA 382

Query: 407 WGIGRDPSIWEDSQTFKPERFDVGCGNNMDFKGYDYRYLPFGGGRRICPGLPMAIVQVPL 466
           WGIGRDP  W D   F PERF     +++DFKG D+  +PFG GRRICPG+P+A   + L
Sbjct: 383 WGIGRDPKRWTDPLKFSPERF---LNSSIDFKGNDFELIPFGAGRRICPGVPLATQFISL 442

Query: 467 ILATLLHNFEWSLPNGEDLSKLDLNGRLGVTLQKYETLKLIPKRR 509
           I+++L+ NF+W  P G D S+L +  + G+TLQK   L ++PK R
Sbjct: 443 IVSSLVQNFDWGFPKGMDPSQLIMEEKFGLTLQKEPPLYIVPKTR 480

BLAST of CsGy5G005800 vs. ExPASy Swiss-Prot
Match: O64899 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia californica OX=3467 GN=CYP80B1 PE=1 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 1.8e-114
Identity = 210/467 (44.97%), Postives = 307/467 (65.74%), Query Frame = 0

Query: 47  LPPGPNPWPILGNLLQIGQNAHISITQFANIYGPLISFKLGAQRLVVASSPAAATAVLKT 106
           LPPGP PWPI+GNLLQ+G+  H    + A  YG + + K+G + +VVAS+ +AA+ +LKT
Sbjct: 28  LPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFTLKMGTETVVVASTSSAASEILKT 87

Query: 107 QDHLLSARYIFQITPDRALHDQHSLVFSSECGDVWKKLRSICKVNLFTAKAIESQAILRE 166
            D +LSARY+FQ    +  H ++S+V+ S+C + WK LR +C+  LFT K IESQA +RE
Sbjct: 88  HDRILSARYVFQSFRVKG-HVENSIVW-SDCTETWKNLRKVCRTELFTQKMIESQAHVRE 147

Query: 167 KKMKELVEFVESKQGSVVEIKDFVFTSAFNILSNLIFSMDLLDFVGD---GFNGIKGPFK 226
           KK +E+VE++  KQG  V+I + +F +  NI  NLIFS ++ + +GD   G +  K    
Sbjct: 148 KKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQNIFE-LGDPNSGSSEFKEYLW 207

Query: 227 KMMDLGLTPNLADFYPLLRRFDLQGLIRKTVIYKNEVNSVWGILIKERREIRCEKG-SAS 286
           +M++LG + N AD++P+L +FDL G  ++       + ++WG +++ER+  +   G  + 
Sbjct: 208 RMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYAIWGAMLQERKLAKKVDGYKSK 267

Query: 287 NDFLDVLIQSGFSDQQIDALIIELLFAATDTTTTTVEWAMSEILKDKDILNKAREEIKKV 346
           NDF+DV + SG +D QI+AL++EL  A T+T+ +T+EWAM+E+ K+  I  K R EI+ V
Sbjct: 268 NDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWAMTELTKNPKITAKIRSEIQTV 327

Query: 347 AKESPAIDESNISKLQYLWSCVKETLRLHPPVPFLLPRLAPMDCEVMGYSIPKDSMIFVN 406
             E  ++ ES+   L YL + VKETLRLHPP P LLPR A   C ++ Y+IPKD  I VN
Sbjct: 328 VGER-SVKESDFPNLPYLEATVKETLRLHPPTPLLLPRRALETCTILNYTIPKDCQIMVN 387

Query: 407 VWGIGRDPSIWEDSQTFKPERFDVGCGNNMDFKGYDYRYLPFGGGRRICPGLPMAIVQVP 466
            WGIGRDP  W D  TF PERF     +++DF+G D+  +PFG GRRICPGLP+A   + 
Sbjct: 388 AWGIGRDPKTWTDPLTFSPERF---LNSSVDFRGNDFSLIPFGAGRRICPGLPIANQFIA 447

Query: 467 LILATLLHNFEWSLPNGEDLSKLDLNGRLGVTLQKYETLKLIPKRRL 510
           L++AT + N +W LPNG  +  L +  + G+TLQK   L ++PK R+
Sbjct: 448 LLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPLFIVPKSRV 487

BLAST of CsGy5G005800 vs. ExPASy Swiss-Prot
Match: O64900 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica OX=3467 GN=CYP80B2 PE=2 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 8.9e-114
Identity = 210/472 (44.49%), Postives = 306/472 (64.83%), Query Frame = 0

Query: 41  SSKKLPLPPGPNPWPILGNLLQIGQNAHISITQFANIYGPLISFKLGAQRLVVASSPAAA 100
           SS    LPPGP PWPI+GNLLQ+G+  H    + A  YG + + K+G + +VVAS+ +AA
Sbjct: 23  SSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFTLKMGTETVVVASTSSAA 82

Query: 101 TAVLKTQDHLLSARYIFQITPDRALHDQHSLVFSSECGDVWKKLRSICKVNLFTAKAIES 160
           + +LKT D +LSARY+FQ    +  H ++S+V+ S+C + WK LR +C+  LFT K IES
Sbjct: 83  SEILKTHDRILSARYVFQSFRVKG-HVENSIVW-SDCTETWKNLRKVCRTELFTQKMIES 142

Query: 161 QAILREKKMKELVEFVESKQGSVVEIKDFVFTSAFNILSNLIFSMDL--LDFVGDGFNGI 220
           QA +REKK +E+VE++  KQG  V+I + +F +  NI  NLIFS ++  L     G +  
Sbjct: 143 QAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQNIFELGXPNSGSSEF 202

Query: 221 KGPFKKMMDLGLTPNLADFYPLLRRFDLQGLIRKTVIYKNEVNSVWGILIKERREIRCEK 280
           K    +M++LG + N AD++P+L +FDL G  ++       + ++WG +++ER+  +   
Sbjct: 203 KEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYAIWGAMLQERKLAKKVD 262

Query: 281 G-SASNDFLDVLIQSGFSDQQIDALIIELLFAATDTTTTTVEWAMSEILKDKDILNKARE 340
           G  + NDF+DV + SG +D QI+AL++EL  A T+T+ +T+EWAM+E+ K+  I  K R 
Sbjct: 263 GYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWAMTELTKNPKITAKLRS 322

Query: 341 EIKKVAKESPAIDESNISKLQYLWSCVKETLRLHPPVPFLLPRLAPMDCEVMGYSIPKDS 400
           E++ V  E  ++ ES+   L YL + VKETLRLHPP P LLPR A   C ++ Y+IPKD 
Sbjct: 323 ELQTVVGER-SVKESDFPNLPYLEATVKETLRLHPPTPLLLPRRALETCTILNYTIPKDC 382

Query: 401 MIFVNVWGIGRDPSIWEDSQTFKPERFDVGCGNNMDFKGYDYRYLPFGGGRRICPGLPMA 460
            I VN WGIGRDP  W D  TF PERF     +++DF+G D+  +PFG GRRICPGLP+A
Sbjct: 383 QIMVNAWGIGRDPKTWIDPLTFSPERF---LNSSVDFRGNDFSLIPFGAGRRICPGLPIA 442

Query: 461 IVQVPLILATLLHNFEWSLPNGEDLSKLDLNGRLGVTLQKYETLKLIPKRRL 510
              + L++AT + N +W LPNG  +  L +  + G+TLQK   L ++PK R+
Sbjct: 443 NQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPLFIVPKSRV 488

BLAST of CsGy5G005800 vs. ExPASy Swiss-Prot
Match: A8CDR5 (Corytuberine synthase OS=Coptis japonica OX=3442 GN=CYP80G2 PE=1 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 1.3e-101
Identity = 204/493 (41.38%), Postives = 312/493 (63.29%), Query Frame = 0

Query: 22  SLLLLLPPIFIILKRIKSPSSKKLPLPPGPNPWPILGNLLQIGQNA----HISITQFANI 81
           +L  L+P I + +  +K P  K   LPPGP+PWP++GNL  +  N     HI++   A  
Sbjct: 6   ALFSLIPVILVFILLLK-PKYKN--LPPGPHPWPLIGNLPILFTNTEVPLHITLANMART 65

Query: 82  YGPLISFKLGAQRLVVASSPAAATAVLKTQDHLLSARYIFQITPDRALHDQHSLVFSSEC 141
           +GP++   LG Q  V+AS+  AA  +LKT D + SAR+I +++     H ++SLV+ S+C
Sbjct: 66  HGPIMILWLGTQPTVMASTAEAAMEILKTHDRIFSARHI-RMSFRLKHHIKYSLVW-SDC 125

Query: 142 GDVWKKLRSICKVNLFTAKAIESQAILREKKMKELVEFVESKQGSVVEIKDFVFTSAFNI 201
            D WK LR I +  +F+ K +++Q+ +RE+K+ EL++F+ SK+G VV+I  FVF +  NI
Sbjct: 126 TDYWKLLRKIVRTEIFSPKMLQAQSHVREQKVAELIDFLRSKEGQVVKISQFVFGTLLNI 185

Query: 202 LSNLIFSMDLLDFVGDGFN--GIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLIRKTVI 261
           L N++FS D+  + GD  +  GI+   ++M+ +G  PN+A+FYP L   DLQGL +K   
Sbjct: 186 LGNVVFSKDVFVY-GDETDKGGIQNLIREMLMIGAEPNVAEFYPSLEELDLQGLKKKCDE 245

Query: 262 YKNEVNSVWGILIKERREIRCEKGSASNDFLDVLIQSGFSDQQIDALIIELLFAATDTTT 321
               V  +W   +KER+  R E+   S D LDVL+ + F+D QI+AL +E     ++T++
Sbjct: 246 RFIRVMKMWEGTVKERKANRNEE---SKDMLDVLLANDFNDAQINALFLETFGPGSETSS 305

Query: 322 TTVEWAMSEILKDKDILNKAREEIKKVAKESPAIDESNISKLQYLWSCVKETLRLHPPVP 381
            T+EW ++E++K    + K R+E+ +V   S  I ES++ +L YL +C+KE +RLHP  P
Sbjct: 306 ATIEWVIAELIKSPKEMAKVRKELNEVVGTS-TIKESDLPQLPYLQACIKEAMRLHPAAP 365

Query: 382 FLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFKPERFDVGCGNNMDFK 441
           FLLPR A   CEVMGY+IPK+S + VN + IGRDP  W+D  TF PERF     +++DF 
Sbjct: 366 FLLPRRAAETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFWPERF---LESDVDFH 425

Query: 442 GYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGRLGVTL 501
           G  Y+++PFG GRR C G+P+A   +PLI+ +L+HN+++ LP G     L +N  L +TL
Sbjct: 426 GAHYQFIPFGSGRRTCVGMPLATRTIPLIVGSLVHNYDFGLPGGNRPEDLKMNEMLSLTL 485

Query: 502 QKYETLKLIPKRR 509
               +L ++PK R
Sbjct: 486 AIDPSLCVVPKAR 485

BLAST of CsGy5G005800 vs. NCBI nr
Match: XP_031741076.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis sativus])

HSP 1 Score: 1007 bits (2604), Expect = 0.0
Identity = 507/510 (99.41%), Postives = 509/510 (99.80%), Query Frame = 0

Query: 1   MLTPTLYFKEMLIPSFSILLPSLLLLLPPIFIILKRIKSPSSKKLPLPPGPNPWPILGNL 60
           MLTPTLYFKEMLIPSFSILLPSLLLLLPPIFIILKRIKSPSSKKLPLPPGPNPWPILGNL
Sbjct: 1   MLTPTLYFKEMLIPSFSILLPSLLLLLPPIFIILKRIKSPSSKKLPLPPGPNPWPILGNL 60

Query: 61  LQIGQNAHISITQFANIYGPLISFKLGAQRLVVASSPAAATAVLKTQDHLLSARYIFQIT 120
           LQIGQNAHISITQFANIYGPLISFKLGAQRLVVASSPAAATAVLKTQDHLLSARYIFQIT
Sbjct: 61  LQIGQNAHISITQFANIYGPLISFKLGAQRLVVASSPAAATAVLKTQDHLLSARYIFQIT 120

Query: 121 PDRALHDQHSLVFSSECGDVWKKLRSICKVNLFTAKAIESQAILREKKMKELVEFVESKQ 180
           PDRALHDQHSLVFSSECGDVWKKLRSICKVNLFTAKAIESQAILREKKMKELVEFVESKQ
Sbjct: 121 PDRALHDQHSLVFSSECGDVWKKLRSICKVNLFTAKAIESQAILREKKMKELVEFVESKQ 180

Query: 181 GSVVEIKDFVFTSAFNILSNLIFSMDLLDFVGDGFNGIKGPFKKMMDLGLTPNLADFYPL 240
           GSVVEIKDFVFTSAFNILSNLIFSMDLLDFVGDGFNGIKGPFKKMMDLGLTPNLADFYPL
Sbjct: 181 GSVVEIKDFVFTSAFNILSNLIFSMDLLDFVGDGFNGIKGPFKKMMDLGLTPNLADFYPL 240

Query: 241 LRRFDLQGLIRKTVIYKNEVNSVWGILIKERREIRCEKGSASNDFLDVLIQSGFSDQQID 300
           LRRFDLQGLIRKTVIYKNEVNSVWGILIKERREIRCEKGSASNDFLDVLIQSGFSDQQID
Sbjct: 241 LRRFDLQGLIRKTVIYKNEVNSVWGILIKERREIRCEKGSASNDFLDVLIQSGFSDQQID 300

Query: 301 ALIIELLFAATDTTTTTVEWAMSEILKDKDILNKAREEIKKVAKESPAIDESNISKLQYL 360
           ALIIELLFAATDTTTTTVEWAMSEILKDKDILNKAREEIKKVAKESPAIDESNISKLQYL
Sbjct: 301 ALIIELLFAATDTTTTTVEWAMSEILKDKDILNKAREEIKKVAKESPAIDESNISKLQYL 360

Query: 361 WSCVKETLRLHPPVPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFK 420
           WSCVKETLRLHPPVPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTF 
Sbjct: 361 WSCVKETLRLHPPVPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFN 420

Query: 421 PERFDVGCGNNMDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGE 480
           PERFDVGCGNN+DFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGE
Sbjct: 421 PERFDVGCGNNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGE 480

Query: 481 DLSKLDLNGRLGVTLQKYETLKLIPKRRLI 510
           DLSKLDLNGRLGVTLQKYETLKLIPKRRL+
Sbjct: 481 DLSKLDLNGRLGVTLQKYETLKLIPKRRLV 510

BLAST of CsGy5G005800 vs. NCBI nr
Match: KAE8647950.1 (hypothetical protein Csa_000545 [Cucumis sativus])

HSP 1 Score: 918 bits (2373), Expect = 0.0
Identity = 461/500 (92.20%), Postives = 476/500 (95.20%), Query Frame = 0

Query: 11  MLIPSFSILLPSLLLLLPPIFIILKRIKSPSSKKLPLPPGPNPWPILGNLLQIGQNAHIS 70
           MLIPSFSILLPSLLLL+PPIFIILKRIKSPS KKLPLPPGPNPWPILGNL QIGQNAHIS
Sbjct: 1   MLIPSFSILLPSLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHIS 60

Query: 71  ITQFANIYGPLISFKLGAQRLVVASSPAAATAVLKTQDHLLSARYIFQITPDRALHDQHS 130
           ITQFANIYGPLIS KLGAQRLVVASSPA ATAVLKT D  LS RYI Q+TPDRAL++QHS
Sbjct: 61  ITQFANIYGPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHS 120

Query: 131 LVFSSECGDVWKKLRSICKVNLFTAKAIESQAILREKKMKELVEFVESKQGSVVEIKDFV 190
           LVFS ECGD WK LRSICK NLFTAKAIESQAILREKKMKELVEF+ESKQGSVV+IKDFV
Sbjct: 121 LVFSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFV 180

Query: 191 FTSAFNILSNLIFSMDLLDFVGDGFNGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLI 250
           FTS FN LSNLIFSMDLLDFVGDGFNGIKG FKKMMDLGLTPNLADFYPLLRRFDLQGL 
Sbjct: 181 FTSVFNTLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLR 240

Query: 251 RKTVIYKNEVNSVWGILIKERREIRCEKGSASNDFLDVLIQSGFSDQQIDALIIELLFAA 310
           +K  IY NEV+S+WGILIKERREI C++GSAS+DFLDVLIQSGFSDQQI+ LI ELLFAA
Sbjct: 241 KKAAIYMNEVDSLWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQINYLINELLFAA 300

Query: 311 TDTTTTTVEWAMSEILKDKDILNKAREEIKKVAKESPAIDESNISKLQYLWSCVKETLRL 370
           TDTTTTTVEWAMSEILKDKDILNKAREEIKKVAKESPAIDESNISKLQYLWSCVKETLRL
Sbjct: 301 TDTTTTTVEWAMSEILKDKDILNKAREEIKKVAKESPAIDESNISKLQYLWSCVKETLRL 360

Query: 371 HPPVPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFKPERFDVGCGN 430
           HPPVPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTF PERFDVGCGN
Sbjct: 361 HPPVPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGN 420

Query: 431 NMDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGR 490
           N+DFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGR
Sbjct: 421 NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGR 480

Query: 491 LGVTLQKYETLKLIPKRRLI 510
           LGVTLQKYETLKLIPKRRL+
Sbjct: 481 LGVTLQKYETLKLIPKRRLV 500

BLAST of CsGy5G005800 vs. NCBI nr
Match: XP_008467223.1 (PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis melo])

HSP 1 Score: 898 bits (2321), Expect = 0.0
Identity = 453/501 (90.42%), Postives = 473/501 (94.41%), Query Frame = 0

Query: 11  MLIPSFSILLPSLLLLLPPIFIILKRIKSPSSKKLPLPPGPNPWPILGNLLQIGQNAHIS 70
           MLIPSFSILLPSLLLLLP IFIILK IKSPSSK LPLPPGPNPWPILGNLLQIGQNAHIS
Sbjct: 1   MLIPSFSILLPSLLLLLP-IFIILKHIKSPSSK-LPLPPGPNPWPILGNLLQIGQNAHIS 60

Query: 71  ITQFANIYGPLISFKLGAQRLVVASSPAAATAVLKTQDHLLSARYIFQITPDRALHDQHS 130
           ITQFANIYGPLIS KLGAQRL+VASSPAAATAVLKTQD LLSARYIFQ+TPDRALHDQHS
Sbjct: 61  ITQFANIYGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHS 120

Query: 131 LVFSSECGDVWKKLRSICKVNLFTAKAIESQAILREKKMKELVEFVESKQGSVVEIKDFV 190
           LVFS ECGD WK LRS+CKVNLFTAKAIESQAILREKKMKELV+F+ESKQGSVVE+KDFV
Sbjct: 121 LVFSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFV 180

Query: 191 FTSAFNILSNLIFSMDLLDFVGDGFNGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLI 250
           FTS FN LSNLIFSMDLLDFVGDGF GIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGL 
Sbjct: 181 FTSVFNTLSNLIFSMDLLDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLK 240

Query: 251 RKTVIYKNEVNSVWGILIKERREIRCEKGSASNDFLDVLIQSGFSDQQIDALIIELLFAA 310
            K  IYKNEV+S+WGILIKERREI C++GSASNDFLDVLIQSGF DQQI+ALIIELL A 
Sbjct: 241 NKADIYKNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIELLSAG 300

Query: 311 TDTTTTTVEWAMSEILKDKDILNKAREEIKKVAKESPAIDESNISKLQYLWSCVKETLRL 370
           TDTTTTTVEWAMSEILKDKDILNK REE+KKV KE+  IDESNIS+LQYLW CVKETLRL
Sbjct: 301 TDTTTTTVEWAMSEILKDKDILNKVREEMKKVTKENRPIDESNISELQYLWRCVKETLRL 360

Query: 371 HPPVPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFKPERFDVGCGN 430
           HPP PFLLPRLAPMDCEVMGYSIPKDSM+FVNVWGIGRDPS+WEDSQTF P+RFDVGCGN
Sbjct: 361 HPPAPFLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGN 420

Query: 431 -NMDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNG 490
            N+DFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATL+HNFEWSLPN EDLSKLDLNG
Sbjct: 421 DNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNG 480

Query: 491 RLGVTLQKYETLKLIPKRRLI 510
           RLGVTLQKYETLKLIPKRR+I
Sbjct: 481 RLGVTLQKYETLKLIPKRRVI 499

BLAST of CsGy5G005800 vs. NCBI nr
Match: KAA0055170.1 (putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis melo var. makuwa])

HSP 1 Score: 897 bits (2319), Expect = 0.0
Identity = 452/501 (90.22%), Postives = 473/501 (94.41%), Query Frame = 0

Query: 11  MLIPSFSILLPSLLLLLPPIFIILKRIKSPSSKKLPLPPGPNPWPILGNLLQIGQNAHIS 70
           MLIPSFSILLPSLLLLLP IFIILK IKSPSSK LPLPPGPNPWPILGNLLQIGQNAHIS
Sbjct: 1   MLIPSFSILLPSLLLLLP-IFIILKHIKSPSSK-LPLPPGPNPWPILGNLLQIGQNAHIS 60

Query: 71  ITQFANIYGPLISFKLGAQRLVVASSPAAATAVLKTQDHLLSARYIFQITPDRALHDQHS 130
           ITQFANIYGPLIS KLGAQRL+VASSPAAATAVLKTQD LLSARYIFQ+TPDRALHDQHS
Sbjct: 61  ITQFANIYGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHS 120

Query: 131 LVFSSECGDVWKKLRSICKVNLFTAKAIESQAILREKKMKELVEFVESKQGSVVEIKDFV 190
           LVFS ECGD WK LRS+CKVNLFTAKAIESQAILREKKMKELV+F+ESKQGSVVE+KDFV
Sbjct: 121 LVFSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFV 180

Query: 191 FTSAFNILSNLIFSMDLLDFVGDGFNGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLI 250
           FTS FN LSNLIFSMDL+DFVGDGF GIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGL 
Sbjct: 181 FTSVFNTLSNLIFSMDLIDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLK 240

Query: 251 RKTVIYKNEVNSVWGILIKERREIRCEKGSASNDFLDVLIQSGFSDQQIDALIIELLFAA 310
            K  IYKNEV+S+WGILIKERREI C++GSASNDFLDVLIQSGF DQQI+ALIIELL A 
Sbjct: 241 NKADIYKNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIELLSAG 300

Query: 311 TDTTTTTVEWAMSEILKDKDILNKAREEIKKVAKESPAIDESNISKLQYLWSCVKETLRL 370
           TDTTTTTVEWAMSEILKDKDILNK REE+KKV KE+  IDESNIS+LQYLW CVKETLRL
Sbjct: 301 TDTTTTTVEWAMSEILKDKDILNKVREEMKKVTKENRPIDESNISELQYLWRCVKETLRL 360

Query: 371 HPPVPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFKPERFDVGCGN 430
           HPP PFLLPRLAPMDCEVMGYSIPKDSM+FVNVWGIGRDPS+WEDSQTF P+RFDVGCGN
Sbjct: 361 HPPAPFLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGN 420

Query: 431 -NMDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNG 490
            N+DFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATL+HNFEWSLPN EDLSKLDLNG
Sbjct: 421 DNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNG 480

Query: 491 RLGVTLQKYETLKLIPKRRLI 510
           RLGVTLQKYETLKLIPKRR+I
Sbjct: 481 RLGVTLQKYETLKLIPKRRVI 499

BLAST of CsGy5G005800 vs. NCBI nr
Match: XP_031741123.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis sativus])

HSP 1 Score: 891 bits (2303), Expect = 0.0
Identity = 450/500 (90.00%), Postives = 466/500 (93.20%), Query Frame = 0

Query: 11  MLIPSFSILLPSLLLLLPPIFIILKRIKSPSSKKLPLPPGPNPWPILGNLLQIGQNAHIS 70
           MLIPSFSILLPSLLLL+PPIFIILKRIKSPS KKLPLPPGPNPWPILGNL QIGQNAHIS
Sbjct: 1   MLIPSFSILLPSLLLLIPPIFIILKRIKSPSLKKLPLPPGPNPWPILGNLPQIGQNAHIS 60

Query: 71  ITQFANIYGPLISFKLGAQRLVVASSPAAATAVLKTQDHLLSARYIFQITPDRALHDQHS 130
           ITQFANIYGPLIS KLGAQRLVVASSPA ATAVLKT D  LS RYI Q+TPDRAL++QHS
Sbjct: 61  ITQFANIYGPLISLKLGAQRLVVASSPATATAVLKTHDRFLSNRYILQMTPDRALYNQHS 120

Query: 131 LVFSSECGDVWKKLRSICKVNLFTAKAIESQAILREKKMKELVEFVESKQGSVVEIKDFV 190
           LVFS ECGD WK LRSICK NLFTAKAIESQAILREKKMKELVEF+ESKQGSVV+IKDFV
Sbjct: 121 LVFSPECGDHWKNLRSICKANLFTAKAIESQAILREKKMKELVEFLESKQGSVVKIKDFV 180

Query: 191 FTSAFNILSNLIFSMDLLDFVGDGFNGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLI 250
           FTS FN LSNLIFSMDLLDFVGDGFNGIKG FKKMMDLGLTPNLADFYPLLRRFDLQGL 
Sbjct: 181 FTSVFNTLSNLIFSMDLLDFVGDGFNGIKGSFKKMMDLGLTPNLADFYPLLRRFDLQGLR 240

Query: 251 RKTVIYKNEVNSVWGILIKERREIRCEKGSASNDFLDVLIQSGFSDQQIDALIIELLFAA 310
           +K  IY NEV+S+WGILIKERREI C++GSAS+DFLDVLIQSGFSDQQI+ LI ELL A 
Sbjct: 241 KKAAIYMNEVDSLWGILIKERREIHCQQGSASSDFLDVLIQSGFSDQQINYLINELLSAG 300

Query: 311 TDTTTTTVEWAMSEILKDKDILNKAREEIKKVAKESPAIDESNISKLQYLWSCVKETLRL 370
           TDTTTTTVEWAMSEILKDK ILNK R+EIKKV KESP IDESNISKLQYLWSCVKETLRL
Sbjct: 301 TDTTTTTVEWAMSEILKDKGILNKVRQEIKKVTKESP-IDESNISKLQYLWSCVKETLRL 360

Query: 371 HPPVPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFKPERFDVGCGN 430
           HPP PFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTF PERFDVGCGN
Sbjct: 361 HPPAPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFNPERFDVGCGN 420

Query: 431 NMDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGR 490
           N+DFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGR
Sbjct: 421 NVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGR 480

Query: 491 LGVTLQKYETLKLIPKRRLI 510
           LGVTLQKYE L+LIPKR  I
Sbjct: 481 LGVTLQKYEALELIPKRNPI 499

BLAST of CsGy5G005800 vs. ExPASy TrEMBL
Match: A0A1S3CT14 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo OX=3656 GN=LOC103504626 PE=3 SV=1)

HSP 1 Score: 898 bits (2321), Expect = 0.0
Identity = 453/501 (90.42%), Postives = 473/501 (94.41%), Query Frame = 0

Query: 11  MLIPSFSILLPSLLLLLPPIFIILKRIKSPSSKKLPLPPGPNPWPILGNLLQIGQNAHIS 70
           MLIPSFSILLPSLLLLLP IFIILK IKSPSSK LPLPPGPNPWPILGNLLQIGQNAHIS
Sbjct: 1   MLIPSFSILLPSLLLLLP-IFIILKHIKSPSSK-LPLPPGPNPWPILGNLLQIGQNAHIS 60

Query: 71  ITQFANIYGPLISFKLGAQRLVVASSPAAATAVLKTQDHLLSARYIFQITPDRALHDQHS 130
           ITQFANIYGPLIS KLGAQRL+VASSPAAATAVLKTQD LLSARYIFQ+TPDRALHDQHS
Sbjct: 61  ITQFANIYGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHS 120

Query: 131 LVFSSECGDVWKKLRSICKVNLFTAKAIESQAILREKKMKELVEFVESKQGSVVEIKDFV 190
           LVFS ECGD WK LRS+CKVNLFTAKAIESQAILREKKMKELV+F+ESKQGSVVE+KDFV
Sbjct: 121 LVFSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFV 180

Query: 191 FTSAFNILSNLIFSMDLLDFVGDGFNGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLI 250
           FTS FN LSNLIFSMDLLDFVGDGF GIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGL 
Sbjct: 181 FTSVFNTLSNLIFSMDLLDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLK 240

Query: 251 RKTVIYKNEVNSVWGILIKERREIRCEKGSASNDFLDVLIQSGFSDQQIDALIIELLFAA 310
            K  IYKNEV+S+WGILIKERREI C++GSASNDFLDVLIQSGF DQQI+ALIIELL A 
Sbjct: 241 NKADIYKNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIELLSAG 300

Query: 311 TDTTTTTVEWAMSEILKDKDILNKAREEIKKVAKESPAIDESNISKLQYLWSCVKETLRL 370
           TDTTTTTVEWAMSEILKDKDILNK REE+KKV KE+  IDESNIS+LQYLW CVKETLRL
Sbjct: 301 TDTTTTTVEWAMSEILKDKDILNKVREEMKKVTKENRPIDESNISELQYLWRCVKETLRL 360

Query: 371 HPPVPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFKPERFDVGCGN 430
           HPP PFLLPRLAPMDCEVMGYSIPKDSM+FVNVWGIGRDPS+WEDSQTF P+RFDVGCGN
Sbjct: 361 HPPAPFLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGN 420

Query: 431 -NMDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNG 490
            N+DFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATL+HNFEWSLPN EDLSKLDLNG
Sbjct: 421 DNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNG 480

Query: 491 RLGVTLQKYETLKLIPKRRLI 510
           RLGVTLQKYETLKLIPKRR+I
Sbjct: 481 RLGVTLQKYETLKLIPKRRVI 499

BLAST of CsGy5G005800 vs. ExPASy TrEMBL
Match: A0A5A7UIU3 (Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G001030 PE=3 SV=1)

HSP 1 Score: 897 bits (2319), Expect = 0.0
Identity = 452/501 (90.22%), Postives = 473/501 (94.41%), Query Frame = 0

Query: 11  MLIPSFSILLPSLLLLLPPIFIILKRIKSPSSKKLPLPPGPNPWPILGNLLQIGQNAHIS 70
           MLIPSFSILLPSLLLLLP IFIILK IKSPSSK LPLPPGPNPWPILGNLLQIGQNAHIS
Sbjct: 1   MLIPSFSILLPSLLLLLP-IFIILKHIKSPSSK-LPLPPGPNPWPILGNLLQIGQNAHIS 60

Query: 71  ITQFANIYGPLISFKLGAQRLVVASSPAAATAVLKTQDHLLSARYIFQITPDRALHDQHS 130
           ITQFANIYGPLIS KLGAQRL+VASSPAAATAVLKTQD LLSARYIFQ+TPDRALHDQHS
Sbjct: 61  ITQFANIYGPLISLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHS 120

Query: 131 LVFSSECGDVWKKLRSICKVNLFTAKAIESQAILREKKMKELVEFVESKQGSVVEIKDFV 190
           LVFS ECGD WK LRS+CKVNLFTAKAIESQAILREKKMKELV+F+ESKQGSVVE+KDFV
Sbjct: 121 LVFSPECGDQWKNLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFV 180

Query: 191 FTSAFNILSNLIFSMDLLDFVGDGFNGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLI 250
           FTS FN LSNLIFSMDL+DFVGDGF GIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGL 
Sbjct: 181 FTSVFNTLSNLIFSMDLIDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLK 240

Query: 251 RKTVIYKNEVNSVWGILIKERREIRCEKGSASNDFLDVLIQSGFSDQQIDALIIELLFAA 310
            K  IYKNEV+S+WGILIKERREI C++GSASNDFLDVLIQSGF DQQI+ALIIELL A 
Sbjct: 241 NKADIYKNEVDSLWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIELLSAG 300

Query: 311 TDTTTTTVEWAMSEILKDKDILNKAREEIKKVAKESPAIDESNISKLQYLWSCVKETLRL 370
           TDTTTTTVEWAMSEILKDKDILNK REE+KKV KE+  IDESNIS+LQYLW CVKETLRL
Sbjct: 301 TDTTTTTVEWAMSEILKDKDILNKVREEMKKVTKENRPIDESNISELQYLWRCVKETLRL 360

Query: 371 HPPVPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFKPERFDVGCGN 430
           HPP PFLLPRLAPMDCEVMGYSIPKDSM+FVNVWGIGRDPS+WEDSQTF P+RFDVGCGN
Sbjct: 361 HPPAPFLLPRLAPMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGN 420

Query: 431 -NMDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNG 490
            N+DFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATL+HNFEWSLPN EDLSKLDLNG
Sbjct: 421 DNVDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNG 480

Query: 491 RLGVTLQKYETLKLIPKRRLI 510
           RLGVTLQKYETLKLIPKRR+I
Sbjct: 481 RLGVTLQKYETLKLIPKRRVI 499

BLAST of CsGy5G005800 vs. ExPASy TrEMBL
Match: A0A6J1ELA6 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata OX=3662 GN=LOC111435609 PE=3 SV=1)

HSP 1 Score: 726 bits (1875), Expect = 5.66e-260
Identity = 372/496 (75.00%), Postives = 416/496 (83.87%), Query Frame = 0

Query: 13  IPSFSILLPSLLLLLPPIFIILKRIKSPSSKKLPLPPGPNPWPILGNLLQIGQNAHISIT 72
           I SFS     LLLLLP IFIIL+ I S SSK+ PLPPGP PWPILGNLLQIGQNAHIS+T
Sbjct: 3   IASFSF----LLLLLPLIFIILRHISS-SSKQPPLPPGPKPWPILGNLLQIGQNAHISMT 62

Query: 73  QFANIYGPLISFKLGAQRLVVASSPAAATAVLKTQDHLLSARYIFQITPDRALHDQHSLV 132
           QF++IYGPLIS KLGAQRLVVASSPAAA A+LKTQD LLSARYIFQ+TPDR LHD  SL+
Sbjct: 63  QFSHIYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLL 122

Query: 133 FSSECGDVWKKLRSICKVNLFTAKAIESQAILREKKMKELVEFVESKQGSVVEIKDFVFT 192
           FS ECGD WK LRSIC+  LFTA+AIESQA LR KKM E+VEF++SKQGSVVEIK+ VF 
Sbjct: 123 FSPECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKELVFI 182

Query: 193 SAFNILSNLIFSMDLLDFVGDGFNGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLIRK 252
           + FN LSN IFS DL+ FVGDGFNGIKGPFK M+DLGL PNLADFYPLLR FDLQGL +K
Sbjct: 183 TVFNTLSNFIFSRDLVGFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKK 242

Query: 253 TVIYKNEVNSVWGILIKERREIRCEKGSASNDFLDVLIQSGFSDQQIDALIIELLFAATD 312
             IYKN+V+S W ILIKERR I  +  SA++DFLDVLIQSGF+D+QI  LI+E+L A TD
Sbjct: 243 AAIYKNQVDSSWEILIKERRTIHSQGVSAASDFLDVLIQSGFTDEQISYLIVEVLSAGTD 302

Query: 313 TTTTTVEWAMSEILKDKDILNKAREEIKKVAKESPAIDESNISKLQYLWSCVKETLRLHP 372
           TTTTTVEWAM+E+LK++DI+N+ REE KK A    AIDES +S+L YLW CVKETLRLHP
Sbjct: 303 TTTTTVEWAMAELLKNRDIMNRVREETKK-AINGGAIDESKLSELHYLWKCVKETLRLHP 362

Query: 373 PVPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFKPERFDVGCGNNM 432
           P PFLLPR AP  CE+MGYS+PK +MIFVNVWGIGRDPSIWED Q+FKPERFD GC  + 
Sbjct: 363 PAPFLLPRYAPEACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFD-GC--DA 422

Query: 433 DFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGRLG 492
           D KG+DYR+LPFGGGRRICPGLPMA VQVPLILATL+HNFEW LPN ED S LDLNGRLG
Sbjct: 423 DLKGHDYRFLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLG 482

Query: 493 VTLQKYETLKLIPKRR 508
           VTLQK E LKL+ K R
Sbjct: 483 VTLQKEEPLKLLSKNR 489

BLAST of CsGy5G005800 vs. ExPASy TrEMBL
Match: A0A6J1I4V7 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita maxima OX=3661 GN=LOC111469167 PE=3 SV=1)

HSP 1 Score: 717 bits (1852), Expect = 1.79e-256
Identity = 366/496 (73.79%), Postives = 413/496 (83.27%), Query Frame = 0

Query: 13  IPSFSILLPSLLLLLPPIFIILKRIKSPSSKKLPLPPGPNPWPILGNLLQIGQNAHISIT 72
           I SFS     LLLLLP IF IL+ I S SSK+ PLPPGPNPWPILGNLLQIGQNAHIS+T
Sbjct: 3   IASFSF----LLLLLPLIFTILRHICS-SSKQPPLPPGPNPWPILGNLLQIGQNAHISMT 62

Query: 73  QFANIYGPLISFKLGAQRLVVASSPAAATAVLKTQDHLLSARYIFQITPDRALHDQHSLV 132
           QF++ YGPLIS KLGAQRLVVASSPAAA A+LKTQD LLSARYIFQ+TPDR LHD  SL+
Sbjct: 63  QFSHFYGPLISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLL 122

Query: 133 FSSECGDVWKKLRSICKVNLFTAKAIESQAILREKKMKELVEFVESKQGSVVEIKDFVFT 192
           FS ECGD WK LRSIC+  LFTA+AIESQA LR KKM ++VEF++SKQGSVVEIK+ VF 
Sbjct: 123 FSPECGDRWKNLRSICRAKLFTARAIESQASLRRKKMNDMVEFLQSKQGSVVEIKELVFI 182

Query: 193 SAFNILSNLIFSMDLLDFVGDGFNGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLIRK 252
           + FN LSN IFS DL+ FVGDGFNGIKG FK ++DLGL PNLADFYPLLR FDLQGL +K
Sbjct: 183 TVFNTLSNFIFSRDLVGFVGDGFNGIKGSFKNIIDLGLIPNLADFYPLLRSFDLQGLRKK 242

Query: 253 TVIYKNEVNSVWGILIKERREIRCEKGSASNDFLDVLIQSGFSDQQIDALIIELLFAATD 312
             IYKN+V+S W ILIKERR I     SA++DFLDVLI+SGF+D+QI  LI+E+L A TD
Sbjct: 243 AAIYKNQVDSSWEILIKERRTIHSRDVSAASDFLDVLIESGFTDEQISYLIVEVLSAGTD 302

Query: 313 TTTTTVEWAMSEILKDKDILNKAREEIKKVAKESPAIDESNISKLQYLWSCVKETLRLHP 372
           TTTTTVEWAM+E+LK++DI+N+ REE KK A    AIDES +S+L YLW CVKETLRLHP
Sbjct: 303 TTTTTVEWAMAELLKNRDIMNRVREETKK-AINGGAIDESKLSELHYLWKCVKETLRLHP 362

Query: 373 PVPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFKPERFDVGCGNNM 432
           P PFLLPR AP  CE+MGYS+PKD+MIFVNVWGIGRDPSIWED  +FKPERFD   G + 
Sbjct: 363 PAPFLLPRYAPEACELMGYSVPKDTMIFVNVWGIGRDPSIWEDPGSFKPERFD---GFDA 422

Query: 433 DFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGRLG 492
           D KG+DYR+LPFGGGRRICPGLPMA VQVPLILATL+HNFEW LPN ED S LDLNGRLG
Sbjct: 423 DLKGHDYRFLPFGGGRRICPGLPMAAVQVPLILATLIHNFEWYLPNDEDASTLDLNGRLG 482

Query: 493 VTLQKYETLKLIPKRR 508
           VTLQK E LKL+ K+R
Sbjct: 483 VTLQKEEPLKLLSKKR 489

BLAST of CsGy5G005800 vs. ExPASy TrEMBL
Match: A0A6J1ER52 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata OX=3662 GN=LOC111435789 PE=3 SV=1)

HSP 1 Score: 710 bits (1833), Expect = 1.34e-253
Identity = 359/488 (73.57%), Postives = 407/488 (83.40%), Query Frame = 0

Query: 22  SLLLLLPPIFIILKRIKSPSSKKLPLPPGPNPWPILGNLLQIGQNAHISITQFANIYGPL 81
           S LLLLP IF ILK I S SSK+ P PPGP PWP+LGNLLQIGQNAHIS+TQF++IYGPL
Sbjct: 7   SFLLLLPLIFAILKHISS-SSKQPPFPPGPKPWPMLGNLLQIGQNAHISMTQFSHIYGPL 66

Query: 82  ISFKLGAQRLVVASSPAAATAVLKTQDHLLSARYIFQITPDRALHDQHSLVFSSECGDVW 141
           IS KLGAQRLVVASSPAAA A+LKTQD LLSARYIFQ+ PDRALHD  SLVFS ECGD W
Sbjct: 67  ISLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMIPDRALHDNCSLVFSPECGDRW 126

Query: 142 KKLRSICKVNLFTAKAIESQAILREKKMKELVEFVESKQGSVVEIKDFVFTSAFNILSNL 201
           K LRSIC+  LFTA+AIESQA LR KKM E+VEF++SKQGSVVEIK+FVF + FN LSN 
Sbjct: 127 KNLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKEFVFITVFNTLSNF 186

Query: 202 IFSMDLLDFVGDGFNGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLIRKTVIYKNEVN 261
           IFS DL+ FVGDGFNGI+G FK ++D  L PNLADFYPLLR FDLQGL +K  IYKN+++
Sbjct: 187 IFSRDLVGFVGDGFNGIEGHFKNVIDWALIPNLADFYPLLRCFDLQGLRKKAAIYKNQID 246

Query: 262 SVWGILIKERREIRCEKGSASNDFLDVLIQSGFSDQQIDALIIELLFAATDTTTTTVEWA 321
           S W ILIKERR I       ++DFLDVLIQSGF+D+QI+ LI+E+L A TDTTTTTVEWA
Sbjct: 247 SSWEILIKERRTIHSRGVLVTSDFLDVLIQSGFTDEQINYLIVEVLSAGTDTTTTTVEWA 306

Query: 322 MSEILKDKDILNKAREEIKKVAKESPAIDESNISKLQYLWSCVKETLRLHPPVPFLLPRL 381
           M+E+LK++DI+N+ REE KK A    AIDES +S+L YLW CVKETLRLHPP PFLLPR 
Sbjct: 307 MAELLKNRDIMNRVREETKK-AINGDAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRY 366

Query: 382 APMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFKPERFDVGCGNNMDFKGYDYRY 441
           AP  CE+MGYS+PK +MI VNVWGIGRDPSIWED Q+FKPERFDV C  + D KG+DYR+
Sbjct: 367 APEACELMGYSVPKHTMIVVNVWGIGRDPSIWEDPQSFKPERFDV-C--DADLKGHDYRF 426

Query: 442 LPFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGRLGVTLQKYETL 501
           LPFGGGRR+CPGLPMA VQVPLILATL+HNFEW LPN ED S LDL GRLGVTLQK E L
Sbjct: 427 LPFGGGRRLCPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLKGRLGVTLQKEEPL 486

Query: 502 KLIPKRRL 509
           KL+PKRR+
Sbjct: 487 KLLPKRRI 489

BLAST of CsGy5G005800 vs. TAIR 10
Match: AT2G45570.1 (cytochrome P450, family 76, subfamily C, polypeptide 2 )

HSP 1 Score: 351.7 bits (901), Expect = 1.0e-96
Identity = 204/521 (39.16%), Postives = 305/521 (58.54%), Query Frame = 0

Query: 6   LYFKEMLIPSFSILLPSLLLLLPPIFIILKRIKSPSSKKLPLPPGPNPWPILGNLLQIGQ 65
           + F++ L P F  +L   +     IF    R +S S K +P PPGP   PI+GN+  +G+
Sbjct: 3   IIFEQALFPLFCFVLSFFI-----IFFTTTRPRS-SRKVVPSPPGPPRLPIIGNIHLVGR 62

Query: 66  NAHISITQFANIYGPLISFKLGAQRLVVASSPAAATAVLKTQDHLLSARYIFQITPDRAL 125
           N H S    +  YGP++S K G+   VV +SP AA  VL+T D +LS+R     TP  ++
Sbjct: 63  NPHHSFADLSKTYGPIMSLKFGSLNTVVVTSPEAAREVLRTYDQILSSR-----TPTNSI 122

Query: 126 ----HDQHSLVFSSECGDVWKKLRSICKVNLFTAKAIESQAILREKKMKELVEFV--ESK 185
               HD+ S+V+       W+ LR +    LF+ + IE+   LRE K+KELV F+   S+
Sbjct: 123 RSINHDKVSVVWLPPSSSRWRLLRKLSATQLFSPQRIEATKTLRENKVKELVSFMSESSE 182

Query: 186 QGSVVEIKDFVFTSAFNILSNLIFSMDLLDFVGDGFNGIKGPFKKMMDLGLTPNLADFYP 245
           +   V+I    F +A NI+SN++FS+DL ++  +     +     +M+    P+ A+F+P
Sbjct: 183 REEAVDISRATFITALNIISNILFSVDLGNYDSNKSGVFQDTVIGVMEAVGNPDAANFFP 242

Query: 246 LLRRFDLQGLIRKTV------IYKNEVNSVWGILIKERREIRCEKGSASNDFLDVLI--- 305
            L   DLQG  RKT+      ++K     +   L ++       K     DF+DVL+   
Sbjct: 243 FLGFLDLQG-NRKTLKACSERLFKVFRGFIDAKLAEKSLRDTNSKDVRERDFVDVLLDLT 302

Query: 306 ---QSGFSDQQIDALIIELLFAATDTTTTTVEWAMSEILKDKDILNKAREEIKKVAKESP 365
              ++  +   I  L+++L  A TDT ++TVEWAM+E+L++ + + KA+ EI  V  +  
Sbjct: 303 EGDEAELNTNDIVHLLLDLFGAGTDTNSSTVEWAMAELLRNPETMVKAQAEIDCVIGQKG 362

Query: 366 AIDESNISKLQYLWSCVKETLRLHPPVPFLLPRLAPMDCEVMGYSIPKDSMIFVNVWGIG 425
            ++ES+IS L YL + VKET RLHP  P L+PR A  D EV+G+ +PKD+ +FVNVW IG
Sbjct: 363 VVEESDISALPYLQAVVKETFRLHPAAPLLVPRKAESDVEVLGFMVPKDTQVFVNVWAIG 422

Query: 426 RDPSIWEDSQTFKPERFDVGCGNNMDFKGYDYRYLPFGGGRRICPGLPMAIVQVPLILAT 485
           RDP++WE+S  FKPERF    G ++D +G DY   PFG GRRICPGLP+A+  VPL+LA+
Sbjct: 423 RDPNVWENSSRFKPERF---LGKDIDLRGRDYELTPFGAGRRICPGLPLAVKTVPLMLAS 482

Query: 486 LLHNFEWSLPNGEDLSKLDLNGRLGVTLQKYETLKLIPKRR 509
           LL++F+W LPNG     LD++   G+TL K   L  +P ++
Sbjct: 483 LLYSFDWKLPNGVGSEDLDMDETFGLTLHKTNPLHAVPVKK 508

BLAST of CsGy5G005800 vs. TAIR 10
Match: AT2G45560.1 (cytochrome P450, family 76, subfamily C, polypeptide 1 )

HSP 1 Score: 342.8 bits (878), Expect = 4.7e-94
Identity = 190/478 (39.75%), Postives = 286/478 (59.83%), Query Frame = 0

Query: 47  LPPGPNPWPILGNLLQIGQNAHISITQFANIYGPLISFKLGAQRLVVASSPAAATAVLKT 106
           LPPGP   PI+GN+  +G++ H S  + +  YGP++S KLG+   VV +SP AA  VL+T
Sbjct: 38  LPPGPPRLPIIGNIHLVGKHPHRSFAELSKTYGPVMSLKLGSLNTVVIASPEAAREVLRT 97

Query: 107 QDHLLSARYIFQITPDRAL----HDQHSLVFSSECGDVWKKLRSICKVNLFTAKAIESQA 166
            D +LSAR     +P  A+    H   SLV+       W+ LR +    L + + IE+  
Sbjct: 98  HDQILSAR-----SPTNAVRSINHQDASLVWLPSSSARWRLLRRLSVTQLLSPQRIEATK 157

Query: 167 ILREKKMKELVEFV--ESKQGSVVEIKDFVFTSAFNILSNLIFSMDLLDF-VGDGFNGIK 226
            LR  K+KELV F+   S +   V+I    F +  NI+SN++FS+DL  +      NG++
Sbjct: 158 ALRMNKVKELVSFISESSDREESVDISRVAFITTLNIISNILFSVDLGSYNAKASINGVQ 217

Query: 227 GPFKKMMDLGLTPNLADFYPLLRRFDLQGLIRKTVIYKNEVNSVW-GIL---IKERREIR 286
                +MD   TP+ A+++P LR  DLQG ++   +    +  V+ G +   I E+    
Sbjct: 218 DTVISVMDAAGTPDAANYFPFLRFLDLQGNVKTFKVCTERLVRVFRGFIDAKIAEKSSQN 277

Query: 287 CEKGSASNDFLDVLI-----QSGFSDQQIDALIIELLFAATDTTTTTVEWAMSEILKDKD 346
             K  + NDF+D L+     +S  S   I+ L++++  A TDT+++T+EWAM+E+LK+  
Sbjct: 278 NPKDVSKNDFVDNLLDYKGDESELSISDIEHLLLDMFTAGTDTSSSTLEWAMTELLKNPK 337

Query: 347 ILNKAREEIKKVAKESPAIDESNISKLQYLWSCVKETLRLHPPVPFLLPRLAPMDCEVMG 406
            + KA+ EI  V  ++  ++ES+ISKL YL + VKET RLH PVP L+PR A  D E++G
Sbjct: 338 TMAKAQAEIDCVIGQNGIVEESDISKLPYLQAVVKETFRLHTPVPLLIPRKAESDAEILG 397

Query: 407 YSIPKDSMIFVNVWGIGRDPSIWEDSQTFKPERFDVGCGNNMDFKGYDYRYLPFGGGRRI 466
           + + KD+ + VNVW IGRDPS+W++   F+PERF    G +MD +G DY   PFG GRRI
Sbjct: 398 FMVLKDTQVLVNVWAIGRDPSVWDNPSQFEPERF---LGKDMDVRGRDYELTPFGAGRRI 457

Query: 467 CPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGRLGVTLQKYETLKLIPKRR 509
           CPG+P+A+  V L+LA+LL++F+W LP G     LD++   G+TL K   L  +P ++
Sbjct: 458 CPGMPLAMKTVSLMLASLLYSFDWKLPKGVLSEDLDMDETFGLTLHKTNPLHAVPVKK 507

BLAST of CsGy5G005800 vs. TAIR 10
Match: AT2G45550.1 (cytochrome P450, family 76, subfamily C, polypeptide 4 )

HSP 1 Score: 335.1 bits (858), Expect = 9.8e-92
Identity = 186/486 (38.27%), Postives = 282/486 (58.02%), Query Frame = 0

Query: 36  RIKSPSSKKLPLPPGPNPWPILGNLLQIGQNAHISITQFANIYGPLISFKLGAQRLVVAS 95
           R +  S +   LPPGP   PI+GN+ Q+G+N H S    A IYGP++S K G    VV +
Sbjct: 27  RSRRSSGRAATLPPGPPRLPIIGNIHQVGKNPHSSFADLAKIYGPIMSLKFGCLNSVVIT 86

Query: 96  SPAAATAVLKTQDHLLSARYIFQITPDRALHDQHSLVFSSECGDVWKKLRSICKVNLFTA 155
           SP AA  VL+T D +LS R           H++ S+++       W+ LR +    +F+ 
Sbjct: 87  SPEAAREVLRTHDQILSGRKSNDSIRCFG-HEEVSVIWLPPSSARWRMLRKLSVTLMFSP 146

Query: 156 KAIESQAILREKKMKELVEFV--ESKQGSVVEIKDFVFTSAFNILSNLIFSMDLLDFVGD 215
           +  E+   LR KK++ELV F+   S++   V+I    +T+  NI+SN++FS+DL  +   
Sbjct: 147 QRTEATKALRMKKVQELVSFMNESSERKEAVDISRASYTTVLNIISNILFSVDLGSYDSK 206

Query: 216 GFNGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLIRKTVIYKNE-----VNSVWGILI 275
             N  +      M+    P+ A+++P +   DLQG  RK +    E             I
Sbjct: 207 KSNEFQDTVIGAMEAAGKPDAANYFPFMGFLDLQG-NRKAMRGLTERLFRVFRGFMDAKI 266

Query: 276 KERREIRCEKGSASNDFLDVLI------QSGFSDQQIDALIIELLFAATDTTTTTVEWAM 335
            E+      K  ++ DFLD L+      ++   +  I+ L++++  A TDT+++T+EWAM
Sbjct: 267 AEKSLGNYSKDVSNRDFLDSLLILNEGDEAELDNNDIEHLLLDMFTAGTDTSSSTLEWAM 326

Query: 336 SEILKDKDILNKAREEIKKVAKESPAIDESNISKLQYLWSCVKETLRLHPPVPFLLPRLA 395
           +E+L++   + KA+ E+ +V  ++  + ES+IS L YL + VKET RLHP  P L+PR A
Sbjct: 327 AELLRNPKTMVKAQAEMDRVLGQNSVVQESDISGLPYLQAVVKETFRLHPAAPLLVPRKA 386

Query: 396 PMDCEVMGYSIPKDSMIFVNVWGIGRDPSIWEDSQTFKPERFDVGCGNNMDFKGYDYRYL 455
             D EV+G+ +PKD+ + VNVW IGRDPS+WE+   F+PERF    G ++D KG DY   
Sbjct: 387 ESDVEVLGFMVPKDTQVLVNVWAIGRDPSVWENPSQFEPERF---MGKDIDVKGRDYELT 446

Query: 456 PFGGGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGRLGVTLQKYETLK 509
           PFGGGRRICPGLP+A+  V L+LA+LL++F+W LPNG     LD++   G+TL +  TL 
Sbjct: 447 PFGGGRRICPGLPLAVKTVSLMLASLLYSFDWKLPNGVVSEDLDMDETFGITLHRTNTLY 506

BLAST of CsGy5G005800 vs. TAIR 10
Match: AT3G52970.1 (cytochrome P450, family 76, subfamily G, polypeptide 1 )

HSP 1 Score: 334.7 bits (857), Expect = 1.3e-91
Identity = 179/480 (37.29%), Postives = 287/480 (59.79%), Query Frame = 0

Query: 47  LPPGPNPWPILGNLLQI-GQNAHISITQFANIYGPLISFKLGAQRLVVASSPAAATAVLK 106
           LPPGPNPWP++GN+ Q+ G   H S+T+ +  +GP+++ ++G+   VV SS   A  + K
Sbjct: 36  LPPGPNPWPVIGNIFQLAGLPPHDSLTKLSRRHGPIMTLRIGSMLTVVISSSEVAREIFK 95

Query: 107 TQDHLLSARYIFQITPDRALHDQHSLVFSSECGDVWKKLRSICKVNLFTAKAIESQAILR 166
             D  L+ R I++        D    + +++ G  W+ LR +C    F  + +++ + +R
Sbjct: 96  KHDAALAGRKIYEAMKGGKSSD--GSLITAQYGAYWRMLRRLCTTQFFVTRRLDAMSDVR 155

Query: 167 EKKMKELVEFVE--SKQGS-VVEIKDFVFTSAFNILSNLIFSMDLLDFVGDGFNGIKGPF 226
            + + +++ FVE   + G+  +++  + F  AFN++ NL+FS DLLD   D   G +  +
Sbjct: 156 SRCVDQMLRFVEEGGQNGTKTIDVGRYFFLMAFNLIGNLMFSRDLLD--PDSKRGSEFFY 215

Query: 227 K--KMMDLGLTPNLADFYPLLRRFDLQGLIRKTVIYKNEVNSVWGILIKERREIRCEKGS 286
              K+M+    PN+ADF+PLLR  D QG+ RKT  +  +   + G  I+ER E+R  + S
Sbjct: 216 HTGKVMEFAGKPNVADFFPLLRFLDPQGIRRKTQFHVEKAFEIAGEFIRERTEVREREKS 275

Query: 287 --ASNDFLDVLIQ-----------SGFSDQQIDALIIELLFAATDTTTTTVEWAMSEILK 346
              + D+LDVL++           S FS + I+ ++ E+  A TDTTT+T+EWA++E+L 
Sbjct: 276 DEKTKDYLDVLLEFRGGDGVDEEPSSFSSRDINVIVFEMFTAGTDTTTSTLEWALAELLH 335

Query: 347 DKDILNKAREEIKKVAKES-PAIDESNISKLQYLWSCVKETLRLHPPVPFLLPRLAPMDC 406
           +   L K + E++   K S   + E ++  L YL + + ETLRLHPP+PFL+P  A   C
Sbjct: 336 NPRTLTKLQTELRTYFKSSNQKLQEEDLPNLPYLSAVIMETLRLHPPLPFLVPHKAMSTC 395

Query: 407 EVMG-YSIPKDSMIFVNVWGIGRDPSIWEDSQTFKPERFDVGCGNNMDFKGYDYRYLPFG 466
            +   Y+IPK++ + VNVW IGRDP  W D   FKPERF +   +  DFKG DY +LPFG
Sbjct: 396 HIFDQYTIPKETQVLVNVWAIGRDPKTWIDPIMFKPERF-ISDPDARDFKGQDYEFLPFG 455

Query: 467 GGRRICPGLPMAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGRLGVTLQKYETLKLIP 506
            GRR+CP LP+A   +PL + +++ +F+W+L NG +  ++D+  R+G+TL+K   L+ IP
Sbjct: 456 SGRRMCPALPLASRVLPLAIGSMVRSFDWALENGLNAEEMDMGERIGITLKKAVPLEAIP 510

BLAST of CsGy5G005800 vs. TAIR 10
Match: AT1G33720.1 (cytochrome P450, family 76, subfamily C, polypeptide 6 )

HSP 1 Score: 330.9 bits (847), Expect = 1.8e-90
Identity = 189/476 (39.71%), Postives = 274/476 (57.56%), Query Frame = 0

Query: 48  PPGPNPWPILGNLLQIGQNAHISITQFANIYGPLISFKLGAQRLVVASSPAAATAVLKTQ 107
           PPGP   PI+GN+  +G+N H S T  +  YGP++S KLG    VV +S  A   VLKT 
Sbjct: 39  PPGPPRLPIIGNIHLVGKNPHHSFTDLSKTYGPVMSLKLGCLNSVVIASRDAVREVLKTH 98

Query: 108 DHLLSARYIFQITPDRALHDQHSLVFSSECGDVWKKLRSICKVNLFTAKAIESQAILREK 167
           D +LS RYI + T     H + S+ +       ++ LR +    LF+ + I++   LR K
Sbjct: 99  DQILSGRYISEATKSNN-HHEFSVGWIHPSSSRFRMLRKLSATQLFSPQCIQATKALRMK 158

Query: 168 KMKELVEFVES--KQGSVVEIKDFVFTSAFNILSNLIFSMDLLDFVGDGFNGIKGPFKKM 227
           K++ELV F+    ++   V+I    F +A NI+SN++FS++L  +     +  +      
Sbjct: 159 KVQELVNFLSESCEREEAVDISHVSFVTALNIISNILFSVNLGSYDSKNSSAFQEMVIGY 218

Query: 228 MDLGLTPNLADFYPLLRRFDLQGLIRKTVIYKNEVNSV----WGILIKERREIRCEKGSA 287
            +    P+LA+F+P +R  DLQG  +K       +  V    +   I E+     EK  +
Sbjct: 219 QESIGNPDLANFFPFMRFLDLQGNSKKMRESSGRLLQVFREFYDARIVEKSSRSVEKDVS 278

Query: 288 SNDFLDVLIQSGFSDQ------QIDALIIELLFAATDTTTTTVEWAMSEILKDKDILNKA 347
           S DFLDVLI     D+      +I+ L++++  A TDT ++TVEWAM+E+L +   + K 
Sbjct: 279 SKDFLDVLIDLQQGDETEINIDEIEHLLLDMFVAGTDTNSSTVEWAMAELLGNPKTMTKV 338

Query: 348 REEIKKVAKESPAIDESNISKLQYLWSCVKETLRLHPPVPFLLPRLAPMDCEVMGYSIPK 407
           ++EI  V  ++    ES+ISKL YL + VKET RLHP  PFLL R A  + E++G+++ K
Sbjct: 339 QDEINHVIGQNGDFQESDISKLPYLKAVVKETFRLHPAAPFLLQRKAETNVEILGFTVLK 398

Query: 408 DSMIFVNVWGIGRDPSIWEDSQTFKPERFDVGCGNNMDFKGYDYRYLPFGGGRRICPGLP 467
           DS + VNVW IGRDP +WE+   F+PERF    G  +D KG DY   PFG GRRICPGLP
Sbjct: 399 DSQVLVNVWAIGRDPLVWENPTHFEPERF---LGKEIDVKGTDYELTPFGAGRRICPGLP 458

Query: 468 MAIVQVPLILATLLHNFEWSLPNGEDLSKLDLNGRLGVTLQKYETLKLIP-KRRLI 511
           +A+  V L+LA+LL+ FEW LPNG     LD+    G+T+ K   L  +P K+R I
Sbjct: 459 LAMKTVHLMLASLLYTFEWKLPNGVGSEDLDMEETFGLTVHKTNPLLAVPLKKRAI 510