CsGy5G002630 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy5G002630
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionTyrosine--tRNA ligase
LocationGy14Chr5: 1701646 .. 1705477 (-)
RNA-Seq ExpressionCsGy5G002630
SyntenyCsGy5G002630
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAAAGAAAAAGAAAAACCCTAGAGAGGTGTGTATTCTTCATTTTATCAGTTTTTACCCATCGCAGCCGCCATCGCCACCCAATCAAGTTTTTTCTAAACCTAACTCCTTGCGGCAGCCACACTCAAATCCTGAAATTGCAAATATTTTGGATCAAGACGAGATTTCAAATTCATTTTGATTTGAAAATTCTTTCAAATCAAAAGGTTCCAAGCAGTGGCTTCAATGGCGACAGAAGCACCTACGCAAGCTCCATCCGAGGACTTACAGTCCCTCTCAGTCTCAGACGCGAAAGTTCAGGAGCCGGCTTGTTCATCCAACTCTTCAAACCAGTATGAAGTTTTTGTGTTTGACACTTATTGTTTTCGTATCCATTTCTCCCTTGATTTGCATCTGAATTGGAATTTACATGACTTGTACTGTATTTTTCTACAGATTGAGTTTGGAGGAGAAGTACCAAATTGTTAGAAGTGTTGGGGAAGAGTGTATTCAAGAAGATGAGCTGCGGAACTTGCTTGATAAGAAGCCGGAACCAGTGTGCTATGATGGGTTCGAACCATCTGGGAGAATGCATATTGCTCAGGTAAATTTTACAGGAATCTTTGAGTTGGGTCCTTAAGTTGTTGTATTTCAATGAATTGTGTTGCGTTTCAATTCGAAACGGTAGCTATAAACTAAGTGACTTGGGTTTTGATTTTCTGTGGCAATCTACCCTGTTCACAACGTGAATTTCAGCAGAAAAAAAAGGGGACCAAGCAATTTTTAGTTTAGTCTAAAACGTAATGGAAATTGAATAATTTCCAAGTGAGTTTCCCCCACCCAAGTGTCCGTTGAATATGAATTGCTGATTTATGTGCCTTTGTTCCCTTACACAGGGAGTGATGAAGGCAATAAATGTCAACAAGCTTACTTCTGCTGGGTGCAGAGTGAAAATCTGGATTGCAGATTGGTTTGCTCAGCTCAACAACAAAATGGGGGGTGATCTGAAGAAAATCGAGACTGTTGGCCGTTATCTAATTGAGATATGGAAAGCAGTTGGGATGGATCTGGAAGGAGGTAAGGTTGAATTTTTGTGGTCTTCAAAGGAGATTAACTCTAGGGCAGGGGAATACTGGCCCCTTGTAATGGATATCGCTAGGAGAAACAAGCTTCCAAGGATAATTAGGTATATATTTCTGAAAGGTGCTCGTTGTGTACTTATGTTTAATCTGCATTCTGCAATAATATTGGCTCATGGTTTGCTTCCTGTCCTCCATCCCCCCTCTGCAGGTGTAGCCAAATTATGGGAAGAAATGAACAGGATGAATTGACTGCTGCTCAAATTCTCTACCCATGCATGCAATGTGCTGACATTTTTTTCCTGAAGGTTTTTTCTCTTTCAACTTTCATGTAGTTTGTTGACATGCATTTTTCTCCTCATATCATAAGGAGACAAAGATGTCACTTGGTATGAATGCTGCATAATATATTTGTGTGGCAATTAAAAGTTCATGGTGATATAGGTTGTCTATTTCGTTATATGCTGGAATGTACGGCGAGATTTTAATGATTTTTTGTTAACCAGATGTTCCATTCCATTGCAACAGGCTGACATCTGTCAACTGGGAATGGATCAACGGAAAGTAAATGTCCTTGCAAGAGAGTATTGCGATGACATCAAGAGGAAAAACAAGCCTATCATCCTATCACATCGTATGCTCTTAAGTCATCAGTTCTTATTAATCTTGCTGATGCTTGTGATATTAGCAAATTAATGTGATGTCAATGGTGGATGCAGATATGTTGCCGGGCTTACAGCAGGGGCAGGAGAAGATGTCTAAAAGTGATCCATCATCCTCTATATTTATGGAAGACGATGAGGTAAGGCATTAGAGGGGAAATTGAGCATCCCATGTGTAGAGGAAGTTAGGGTGGCGGTGCAGTTTTGCTAGCGTGTAGGTTTGATAGATCTAAATAAGAATCCCAGGTGCAGAGGGGAATAAAAATTACATCTTTATTCTTATGATTGTGTATAGTTATAGGCTGACTTGACCGAGTGTTTGATTCTGAGGCTAGCTTGTGATATTGTAGATCTGTTTTCTAACTTGTTCATCTGGCCACATTAGGCAGAAGTGAATTTAAAGATAAAGAAAGCATATTGCCCTCCATTGATCGTGGAAGGGAATCCATGCTTAGAATACATAAAGTACCTTATTTTCCCTTGGTTCAAGGAGTTCACCGTGGAACGAAATGCAGATCATGGTGGAAATAAGTAAGTTTGAGGAGATTTATAAATTTATTCTTGTTTCTTTCTCCTCCTATATCACGATCCTAAATTTTAGTTATAAAATTTGCTGTGACCTCCTATTCATTTATTGTTTTGAAATATTGCAGGACCTTCTCAACCTTCGAAGAATTGGTTTCTGACTACGCAGCAGGAGAACTGCATCCAGGTGATCTCAAACCAGCGTTGTCTAAGGCTTTGAATAAGATATTGGAGGTATGTCATTTCTGCACAATATTATTCCATGAGTACATACCGTTATTGGAATTGGTTGTAGTCATCACTTTGCTTTACTACAGTACAATACACTATTATGGTTTTTGTGCAAACAATCTTATTAGATCATCATGAGTACCTAATTGCAAAATGCAAATTGGTCTATGATGCCGTTGGTAGAGACTAGAAAGCCATAAATAACCATCAATTCAGTACTTTGGAGTTTGGCTAGTGCTAAGTCAACCGTTGTGTCTTCTCCTCCTGACACATATGGTTTCTTTAGCATTTATTCACGTTCTTGCAATTTCATCTGACGAAACGTTAAACATCTAAGCATAACTTAAGAAGCTTCATATTTTCATGAAAATACAAACAACTTTTCAGTGAAAAGGGTAGTAATACAGCTAGGAATTTACTTGATTCTTATTACCCTCAGCTACCTATAAAGCTATGATTGATTTCATTCCATCTGCAGCCCGTACGAGATCACTTCAAAAATGATAGCAACGCCAAAGATTTATTGAAGAGGGTCAAGGTATGTGTTTGTCTTAACGACTCTTAAAATTATTTTGGCTAAATTACAAAGAGTAAACTCACTTTGCCATTTGCTTAAAAATACTCATATTTTTCAGAAGTTGTAGTATTACTCTTGATCCATAAATGTCTCGTGACTACTCCTTGGATTAGAAATCCATTAATTTTTTTTTTATGAAAATTGGGCAATAGAATGGTCTGGCCATGACCTAGAAAAGAAATTTGGAAAGCCACATGTATAGTTTTGAAGTATGTATGTAAATGGTAATACTGCAACATTTAGAAGATTTGAGGTTTTATTGAAGTAAAGGCAAGGTTTAGTGGTACTTTTTCATCCATTATGCACATGGCCCAATTCCCTGGGTTTTCACTGTGGCCTGAACTAAGAAGATTTAAGAACTGCTCATTAGAAATTAATTCTCAAATTCGAAATGTATTAATTTTACCTGTAGGAAAATTTATCTAGAGCTGTTTATCGTGTTATGCAGGCGTACCGAGTCACTAGGTGAAGAATGGTCCTATTTTCTCCTGCCCAATTTGTGGATGGAACACCTACCCCCAACCATTTCAACAGGGAAATTATTGCGGATTTTGTTTTATTTTTGTCCTTTTATGGTCAACCTACAAATTTTAAGCTGCTTTAATATTTGAATGCGGATAGATATGTCAAGCTCCAAAACTCATGTCACTTCTTGATGAGAAACAAGCAAATGAGAAGTTCGTATTGAATGATGAAAAAAGGACTTAATGGATAATTCCATCTTAAATGAATTTTGAAGTATGGTTTAAAGTGCTAT

mRNA sequence

AAAAAAAAGAAAAAGAAAAACCCTAGAGAGGTGTGTATTCTTCATTTTATCAGTTTTTACCCATCGCAGCCGCCATCGCCACCCAATCAAGTTTTTTCTAAACCTAACTCCTTGCGGCAGCCACACTCAAATCCTGAAATTGCAAATATTTTGGATCAAGACGAGATTTCAAATTCATTTTGATTTGAAAATTCTTTCAAATCAAAAGGTTCCAAGCAGTGGCTTCAATGGCGACAGAAGCACCTACGCAAGCTCCATCCGAGGACTTACAGTCCCTCTCAGTCTCAGACGCGAAAGTTCAGGAGCCGGCTTGTTCATCCAACTCTTCAAACCAATTGAGTTTGGAGGAGAAGTACCAAATTGTTAGAAGTGTTGGGGAAGAGTGTATTCAAGAAGATGAGCTGCGGAACTTGCTTGATAAGAAGCCGGAACCAGTGTGCTATGATGGGTTCGAACCATCTGGGAGAATGCATATTGCTCAGGGAGTGATGAAGGCAATAAATGTCAACAAGCTTACTTCTGCTGGGTGCAGAGTGAAAATCTGGATTGCAGATTGGTTTGCTCAGCTCAACAACAAAATGGGGGGTGATCTGAAGAAAATCGAGACTGTTGGCCGTTATCTAATTGAGATATGGAAAGCAGTTGGGATGGATCTGGAAGGAGGTAAGGTTGAATTTTTGTGGTCTTCAAAGGAGATTAACTCTAGGGCAGGGGAATACTGGCCCCTTGTAATGGATATCGCTAGGAGAAACAAGCTTCCAAGGATAATTAGGTGTAGCCAAATTATGGGAAGAAATGAACAGGATGAATTGACTGCTGCTCAAATTCTCTACCCATGCATGCAATGTGCTGACATTTTTTTCCTGAAGGCTGACATCTGTCAACTGGGAATGGATCAACGGAAAGTAAATGTCCTTGCAAGAGAGTATTGCGATGACATCAAGAGGAAAAACAAGCCTATCATCCTATCACATCATATGTTGCCGGGCTTACAGCAGGGGCAGGAGAAGATGTCTAAAAGTGATCCATCATCCTCTATATTTATGGAAGACGATGAGGCAGAAGTGAATTTAAAGATAAAGAAAGCATATTGCCCTCCATTGATCGTGGAAGGGAATCCATGCTTAGAATACATAAAGTACCTTATTTTCCCTTGGTTCAAGGAGTTCACCGTGGAACGAAATGCAGATCATGGTGGAAATAAGACCTTCTCAACCTTCGAAGAATTGGTTTCTGACTACGCAGCAGGAGAACTGCATCCAGGTGATCTCAAACCAGCGTTGTCTAAGGCTTTGAATAAGATATTGGAGGTATGTCATTTCTGCACAATATTATTCCATGAGTACATACCGTTATTGGAATTGGTTGTAGTCATCACTTTGCTTTACTACAGTACAATACACTATTATGGTTTTTGTGCAAACAATCTTATTAGATCATCATGAGTACCTAATTGCAAAATGCAAATTGGTCTATGATGCCGTTGGTAGAGACTAGAAAGCCATAAATAACCATCAATTCAGTACTTTGGAGTTTGGCTAGTGCTAAGTCAACCGTTGTGTCTTCTCCTCCTGACACATATGGTTTCTTTAGCATTTATTCACGTTCTTGCAATTTCATCTGACGAAACGTTAAACATCTAAGCATAACTTAAGAAGCTTCATATTTTCATGAAAATACAAACAACTTTTCAGTGAAAAGGGTAGTAATACAGCTAGGAATTTACTTGATTCTTATTACCCTCAGCTACCTATAAAGCTATGATTGATTTCATTCCATCTGCAGCCCGTACGAGATCACTTCAAAAATGATAGCAACGCCAAAGATTTATTGAAGAGGGTCAAGGCGTACCGAGTCACTAGGTGAAGAATGGTCCTATTTTCTCCTGCCCAATTTGTGGATGGAACACCTACCCCCAACCATTTCAACAGGGAAATTATTGCGGATTTTGTTTTATTTTTGTCCTTTTATGGTCAACCTACAAATTTTAAGCTGCTTTAATATTTGAATGCGGATAGATATGTCAAGCTCCAAAACTCATGTCACTTCTTGATGAGAAACAAGCAAATGAGAAGTTCGTATTGAATGATGAAAAAAGGACTTAATGGATAATTCCATCTTAAATGAATTTTGAAGTATGGTTTAAAGTGCTAT

Coding sequence (CDS)

ATGGCGACAGAAGCACCTACGCAAGCTCCATCCGAGGACTTACAGTCCCTCTCAGTCTCAGACGCGAAAGTTCAGGAGCCGGCTTGTTCATCCAACTCTTCAAACCAATTGAGTTTGGAGGAGAAGTACCAAATTGTTAGAAGTGTTGGGGAAGAGTGTATTCAAGAAGATGAGCTGCGGAACTTGCTTGATAAGAAGCCGGAACCAGTGTGCTATGATGGGTTCGAACCATCTGGGAGAATGCATATTGCTCAGGGAGTGATGAAGGCAATAAATGTCAACAAGCTTACTTCTGCTGGGTGCAGAGTGAAAATCTGGATTGCAGATTGGTTTGCTCAGCTCAACAACAAAATGGGGGGTGATCTGAAGAAAATCGAGACTGTTGGCCGTTATCTAATTGAGATATGGAAAGCAGTTGGGATGGATCTGGAAGGAGGTAAGGTTGAATTTTTGTGGTCTTCAAAGGAGATTAACTCTAGGGCAGGGGAATACTGGCCCCTTGTAATGGATATCGCTAGGAGAAACAAGCTTCCAAGGATAATTAGGTGTAGCCAAATTATGGGAAGAAATGAACAGGATGAATTGACTGCTGCTCAAATTCTCTACCCATGCATGCAATGTGCTGACATTTTTTTCCTGAAGGCTGACATCTGTCAACTGGGAATGGATCAACGGAAAGTAAATGTCCTTGCAAGAGAGTATTGCGATGACATCAAGAGGAAAAACAAGCCTATCATCCTATCACATCATATGTTGCCGGGCTTACAGCAGGGGCAGGAGAAGATGTCTAAAAGTGATCCATCATCCTCTATATTTATGGAAGACGATGAGGCAGAAGTGAATTTAAAGATAAAGAAAGCATATTGCCCTCCATTGATCGTGGAAGGGAATCCATGCTTAGAATACATAAAGTACCTTATTTTCCCTTGGTTCAAGGAGTTCACCGTGGAACGAAATGCAGATCATGGTGGAAATAAGACCTTCTCAACCTTCGAAGAATTGGTTTCTGACTACGCAGCAGGAGAACTGCATCCAGGTGATCTCAAACCAGCGTTGTCTAAGGCTTTGAATAAGATATTGGAGGTATGTCATTTCTGCACAATATTATTCCATGAGTACATACCGTTATTGGAATTGGTTGTAGTCATCACTTTGCTTTACTACAGTACAATACACTATTATGGTTTTTGTGCAAACAATCTTATTAGATCATCATGA

Protein sequence

MATEAPTQAPSEDLQSLSVSDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELRNLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGGDLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPRIIRCSQIMGRNEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCLEYIKYLIFPWFKEFTVERNADHGGNKTFSTFEELVSDYAAGELHPGDLKPALSKALNKILEVCHFCTILFHEYIPLLELVVVITLLYYSTIHYYGFCANNLIRSS*
Homology
BLAST of CsGy5G002630 vs. ExPASy Swiss-Prot
Match: Q8S9J2 (Tyrosine--tRNA ligase 1, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At2g33840 PE=2 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 3.5e-161
Identity = 272/355 (76.62%), Postives = 312/355 (87.89%), Query Frame = 0

Query: 9   APSEDLQSLSVSDAKVQEPACSSNSSNQLS--LEEKYQIVRSVGEECIQEDELRNLLDKK 68
           A S +++++SVS  +    A SS+   Q+S  +E++Y+IVRS+GEECIQE+EL+NLL KK
Sbjct: 11  ALSSEMEAVSVSSTQ----ASSSSDGFQMSEEVEKRYKIVRSIGEECIQEEELKNLLAKK 70

Query: 69  PEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGGDLKKIE 128
             P+CYDGFEPSGRMHIAQGVMK INVNK+TSAGCRVKIWIADWFAQLNNKMGGDLKKI 
Sbjct: 71  AAPICYDGFEPSGRMHIAQGVMKVINVNKMTSAGCRVKIWIADWFAQLNNKMGGDLKKIR 130

Query: 129 TVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPRIIRCSQI 188
            VG Y  EIWKA GMD    KVEFLWSS+EINS+A +YWPLVMDIAR+NKLPRI+RC QI
Sbjct: 131 VVGEYFQEIWKAAGMD--NDKVEFLWSSEEINSKADKYWPLVMDIARKNKLPRILRCVQI 190

Query: 189 MGRNEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKRKNKPII 248
           MGR+E DEL+AAQILYPCMQCADIFFL+ADICQLGMDQRKVNVLAREYCDDIKRKNKPII
Sbjct: 191 MGRSETDELSAAQILYPCMQCADIFFLEADICQLGMDQRKVNVLAREYCDDIKRKNKPII 250

Query: 249 LSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCLEYIKYL 308
           LSHHMLPGLQQGQEKMSKSDP S+IFMED+EAEVN+KIKKAYCPP +V+GNPCLEYIKY+
Sbjct: 251 LSHHMLPGLQQGQEKMSKSDPLSAIFMEDEEAEVNVKIKKAYCPPKVVKGNPCLEYIKYI 310

Query: 309 IFPWFKEFTVERNADHGGNKTFSTFEELVSDYAAGELHPGDLKPALSKALNKILE 362
           I PWF EFTVERN ++GGNKT+ +FE++ +DY +GELHPGDLK  L  ALNKIL+
Sbjct: 311 ILPWFDEFTVERNEEYGGNKTYKSFEDIAADYESGELHPGDLKKGLMNALNKILQ 359

BLAST of CsGy5G002630 vs. ExPASy Swiss-Prot
Match: F4HWL4 (Tyrosine--tRNA ligase 2, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At1g28350 PE=1 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 4.1e-146
Identity = 252/348 (72.41%), Postives = 288/348 (82.76%), Query Frame = 0

Query: 16  SLSVSDAKVQEPACSSNSSNQLS--LEEKYQIVRSVGEECIQEDELRNLLDKKPEPVCYD 75
           S    D  +   A SS +  Q+S   E KY+IVRS+GEECIQEDEL+NLL KKP P+CYD
Sbjct: 378 STKEEDLSINTSASSSAAGLQMSEEAEMKYKIVRSIGEECIQEDELKNLLAKKPAPICYD 437

Query: 76  GFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGGDLKKIETVGRYLI 135
           GFEPSGRMHIAQGVMK  NVNKLTSAGC+VKIWIADWFAQLNNK+GGDL++I  VG Y  
Sbjct: 438 GFEPSGRMHIAQGVMKVTNVNKLTSAGCQVKIWIADWFAQLNNKLGGDLERIRVVGEYFK 497

Query: 136 EIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPRIIRCSQIMGRNEQD 195
           EIW+A GM+    KVEFLW+S EIN +  +YWPLVMDIARRN L RI+RC QIMGR+E +
Sbjct: 498 EIWQAGGMN--NDKVEFLWASDEINGKGSKYWPLVMDIARRNNLRRILRCGQIMGRSETE 557

Query: 196 ELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKRKNKPIILSHHMLP 255
            L+AAQILYPCMQCADIF L+ADICQLGMDQRKVN+LAREYC DIKRKNKPIILSHHMLP
Sbjct: 558 VLSAAQILYPCMQCADIFLLEADICQLGMDQRKVNMLAREYCADIKRKNKPIILSHHMLP 617

Query: 256 GLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCLEYIKYLIFPWFKE 315
           GLQQGQEKMSKSDPSS+IFMED+EA+VN KI KAYCPP  VEGNPCLEY+KY++ P F E
Sbjct: 618 GLQQGQEKMSKSDPSSAIFMEDEEADVNEKISKAYCPPKTVEGNPCLEYVKYIVLPRFNE 677

Query: 316 FTVERNADHGGNKTFSTFEELVSDYAAGELHPGDLKPALSKALNKILE 362
           F VE +  +GGNKTF++FE++V+DY  GELHP DLK AL KALN  L+
Sbjct: 678 FKVE-SEKNGGNKTFNSFEDIVADYETGELHPEDLKKALMKALNITLQ 722

BLAST of CsGy5G002630 vs. ExPASy Swiss-Prot
Match: Q5UPJ7 (Tyrosine--tRNA ligase OS=Acanthamoeba polyphaga mimivirus OX=212035 GN=YARS PE=1 SV=1)

HSP 1 Score: 263.8 bits (673), Expect = 3.1e-69
Identity = 143/326 (43.87%), Postives = 204/326 (62.58%), Query Frame = 0

Query: 40  EEKYQIVRSVGEECIQEDELRNLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSA 99
           E +   + S+ EEC   D L+ L+D       Y+GFEPSGR+HIAQ ++  +N N +   
Sbjct: 10  EHRLTQLLSIAEECETLDRLKQLVDSGRIFTAYNGFEPSGRIHIAQALITVMNTNNIIEC 69

Query: 100 GCRVKIWIADWFAQLNNKMGGDLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINS 159
           G ++ I+IADWFA++N KM GD+ KI  +GRY IE++KA G++L+G    F+W+S+ I S
Sbjct: 70  GGQMIIYIADWFAKMNLKMNGDINKIRELGRYFIEVFKACGINLDG--TRFIWASEFIAS 129

Query: 160 RAGEYWPLVMDIARRNKLPRIIRCSQIMGRNEQDELTAAQILYPCMQCADIFFL---KAD 219
               Y   ++DIA  + + R+ RC QIMGRNE D L A+QI YPCMQ AD+F L     D
Sbjct: 130 NP-SYIERMLDIAEFSTISRVKRCCQIMGRNESDCLKASQIFYPCMQAADVFELVPEGID 189

Query: 220 ICQLGMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDD 279
           ICQLG+DQRKVN+LA EY +D +    PI LSHHML  L   ++KMSKSDP  +IFM+D 
Sbjct: 190 ICQLGIDQRKVNMLAIEYAND-RGLKIPISLSHHMLMSLSGPKKKMSKSDPQGAIFMDDT 249

Query: 280 EAEVNLKIKKAYCPPLIVEGNPCLEYIKYLIFPWFKEFTVERNADHGGNKTFSTFEELVS 339
           E EV+ KI +AYC     + NP  EYIKYL+  WF    +         K ++  E +  
Sbjct: 250 EQEVSEKISRAYCTDETFD-NPIFEYIKYLLLRWFGTLNL-------CGKIYTDIESIQE 309

Query: 340 DYAAGELHPGDLKPALSKALNKILEV 363
           D+++  ++  +LK  ++  +N I+++
Sbjct: 310 DFSS--MNKRELKTDVANYINTIIDL 321

BLAST of CsGy5G002630 vs. ExPASy Swiss-Prot
Match: Q5JF63 (Tyrosine--tRNA ligase OS=Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) OX=69014 GN=tyrS PE=3 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 1.7e-59
Identity = 131/354 (37.01%), Postives = 205/354 (57.91%), Query Frame = 0

Query: 37  LSLEEKYQIV-RSVGEECIQEDELRNLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNK 96
           + +E K +++ +   EE + E+ LR+LL+       Y GFE SG +H+  G+M    +  
Sbjct: 1   MDIERKIELIKKKPTEELLTEENLRHLLEVGAPLQHYIGFEISGYIHLGTGLMAGAKIAD 60

Query: 97  LTSAGCRVKIWIADWFAQLNNKMGGDLKKIETVGRYLIEIWKAVGMDLEGG---KVEFLW 156
           L  AG + +I++ADW + +N+K+GGDL+ I+ V     +      + + GG   KVEF+ 
Sbjct: 61  LQKAGVKTRIFLADWHSWINDKLGGDLEVIQKVALTYFKEGMKQSIKVMGGDPDKVEFVL 120

Query: 157 SSKEINSRAGEYWPLVMDIARRNKLPRIIRCSQIMGRNEQDELTAAQILYPCMQCADIFF 216
           +S+ ++   G+YW  V+DI++   L R++R   IMGR   + +  A+++YP MQ ADIF+
Sbjct: 121 ASEILDK--GDYWQTVIDISKNVTLARMLRSITIMGRQMGEAIDFAKLIYPAMQVADIFY 180

Query: 217 LKADICQLGMDQRKVNVLAREYCDDIK--------RKNKPIILSHHMLPGLQQ------- 276
               I   GMDQRK +V+A E    +K         K KP+ L HH+L GLQ+       
Sbjct: 181 QGVTIAHAGMDQRKAHVIAIEVAQKLKYHPLEWKGEKLKPVALHHHLLLGLQEPPVWPIE 240

Query: 277 ---------GQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCLEYIKYLIF 336
                     Q KMSKS P S++F+ D   E+  K++KA+CP   V+ NP L++ +Y+IF
Sbjct: 241 SEEQFKELKTQMKMSKSKPYSAVFIHDSPEEIKQKLRKAFCPAREVKYNPVLDWAEYIIF 300

Query: 337 -PWFKEFTVERNADHGGNKTFSTFEELVSDYAAGELHPGDLKPALSKALNKILE 362
                EFT+ R A  GG+ T++TFEEL  D+A G+LHP DLK A+++ L ++L+
Sbjct: 301 REEPTEFTIHRPAKFGGDVTYTTFEELKKDFAEGKLHPLDLKNAVAEYLIELLK 352

BLAST of CsGy5G002630 vs. ExPASy Swiss-Prot
Match: Q8U2H3 (Tyrosine--tRNA ligase OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) OX=186497 GN=tyrS PE=3 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 2.9e-59
Identity = 126/354 (35.59%), Postives = 206/354 (58.19%), Query Frame = 0

Query: 37  LSLEEKYQIV-RSVGEECIQEDELRNLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNK 96
           + +EE+ ++V +   EE +  + LR+L +       Y GFE SG +H+  G+M    +  
Sbjct: 1   MDIEERIKLVLKKPTEEVLTVENLRHLFEIGAPLQHYIGFEISGYIHLGTGLMAGAKIAD 60

Query: 97  LTSAGCRVKIWIADWFAQLNNKMGGDLKKIETVGRYLIEIWKAVGMDLEGG---KVEFLW 156
              AG + ++++ADW + +N+K+GGDL+ I+ V     ++     +++ GG   KVEF+ 
Sbjct: 61  FQKAGIKTRVFLADWHSWINDKLGGDLETIQEVALKYFKVGMEKSIEVMGGKPEKVEFVL 120

Query: 157 SSKEINSRAGEYWPLVMDIARRNKLPRIIRCSQIMGRNEQDELTAAQILYPCMQCADIFF 216
           +S+ +    G+YW  V+DI++   L R++R   IMGR   + +  A+++YP MQ ADIF+
Sbjct: 121 ASEILEK--GDYWQTVIDISKNVTLSRVLRSITIMGRKMGEAIDFAKLIYPMMQVADIFY 180

Query: 217 LKADICQLGMDQRKVNVLAREYCDDIK--------RKNKPIILSHHMLPGLQQ------- 276
               I   GMDQRK +V+A E  + ++         K KP+ + HH+L GLQ+       
Sbjct: 181 QGVTIAHAGMDQRKAHVIAIEVAEKLRYHPIIHNGEKLKPVAVHHHLLLGLQEPPKWPIE 240

Query: 277 ---------GQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCLEYIKYLIF 336
                     + KMSKS P S++F+ D   E+  K++KA+CP   V+ NP L++++YL+F
Sbjct: 241 SEEEFKEIKAEMKMSKSKPYSAVFIHDSPEEIREKLRKAFCPAREVKYNPVLDWVEYLVF 300

Query: 337 -PWFKEFTVERNADHGGNKTFSTFEELVSDYAAGELHPGDLKPALSKALNKILE 362
                EFT+ R A +GG+ T++TFEEL  D+A G+LHP DLK A+++ L  +LE
Sbjct: 301 REEPTEFTIHRPAKYGGDVTYTTFEELKRDFAEGKLHPLDLKNAVAEYLIDLLE 352

BLAST of CsGy5G002630 vs. NCBI nr
Match: XP_004143904.1 (tyrosine--tRNA ligase 1, cytoplasmic [Cucumis sativus] >KGN50094.1 hypothetical protein Csa_000293 [Cucumis sativus])

HSP 1 Score: 731 bits (1886), Expect = 8.59e-265
Identity = 361/361 (100.00%), Postives = 361/361 (100.00%), Query Frame = 0

Query: 1   MATEAPTQAPSEDLQSLSVSDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR 60
           MATEAPTQAPSEDLQSLSVSDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR
Sbjct: 1   MATEAPTQAPSEDLQSLSVSDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR 60

Query: 61  NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG 120
           NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG
Sbjct: 61  NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG 120

Query: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPRI 180
           DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPRI
Sbjct: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPRI 180

Query: 181 IRCSQIMGRNEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR 240
           IRCSQIMGRNEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR
Sbjct: 181 IRCSQIMGRNEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR 240

Query: 241 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL 300
           KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL
Sbjct: 241 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL 300

Query: 301 EYIKYLIFPWFKEFTVERNADHGGNKTFSTFEELVSDYAAGELHPGDLKPALSKALNKIL 360
           EYIKYLIFPWFKEFTVERNADHGGNKTFSTFEELVSDYAAGELHPGDLKPALSKALNKIL
Sbjct: 301 EYIKYLIFPWFKEFTVERNADHGGNKTFSTFEELVSDYAAGELHPGDLKPALSKALNKIL 360

BLAST of CsGy5G002630 vs. NCBI nr
Match: XP_008437283.1 (PREDICTED: tyrosine--tRNA ligase 1, cytoplasmic [Cucumis melo])

HSP 1 Score: 725 bits (1872), Expect = 1.17e-262
Identity = 357/361 (98.89%), Postives = 360/361 (99.72%), Query Frame = 0

Query: 1   MATEAPTQAPSEDLQSLSVSDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR 60
           MATEAP+QAPSEDLQSLS+SDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR
Sbjct: 1   MATEAPSQAPSEDLQSLSISDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR 60

Query: 61  NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG 120
           NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG
Sbjct: 61  NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG 120

Query: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPRI 180
           DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPRI
Sbjct: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPRI 180

Query: 181 IRCSQIMGRNEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR 240
           IRCSQIMGRNEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR
Sbjct: 181 IRCSQIMGRNEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR 240

Query: 241 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL 300
           KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVE NPCL
Sbjct: 241 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEANPCL 300

Query: 301 EYIKYLIFPWFKEFTVERNADHGGNKTFSTFEELVSDYAAGELHPGDLKPALSKALNKIL 360
           EYIKYLIFPWFKEFTVERNADHGGNKTF+TFEELVSDYAAGELHPGDLKPALSKALNKIL
Sbjct: 301 EYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVSDYAAGELHPGDLKPALSKALNKIL 360

BLAST of CsGy5G002630 vs. NCBI nr
Match: KAA0042754.1 (tyrosine--tRNA ligase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 725 bits (1872), Expect = 6.07e-262
Identity = 357/361 (98.89%), Postives = 360/361 (99.72%), Query Frame = 0

Query: 1   MATEAPTQAPSEDLQSLSVSDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR 60
           MATEAP+QAPSEDLQSLS+SDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR
Sbjct: 43  MATEAPSQAPSEDLQSLSISDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR 102

Query: 61  NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG 120
           NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG
Sbjct: 103 NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG 162

Query: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPRI 180
           DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPRI
Sbjct: 163 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPRI 222

Query: 181 IRCSQIMGRNEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR 240
           IRCSQIMGRNEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR
Sbjct: 223 IRCSQIMGRNEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR 282

Query: 241 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL 300
           KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVE NPCL
Sbjct: 283 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEANPCL 342

Query: 301 EYIKYLIFPWFKEFTVERNADHGGNKTFSTFEELVSDYAAGELHPGDLKPALSKALNKIL 360
           EYIKYLIFPWFKEFTVERNADHGGNKTF+TFEELVSDYAAGELHPGDLKPALSKALNKIL
Sbjct: 343 EYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVSDYAAGELHPGDLKPALSKALNKIL 402

BLAST of CsGy5G002630 vs. NCBI nr
Match: XP_038906448.1 (tyrosine--tRNA ligase 1, cytoplasmic [Benincasa hispida])

HSP 1 Score: 711 bits (1835), Expect = 5.07e-257
Identity = 350/361 (96.95%), Postives = 355/361 (98.34%), Query Frame = 0

Query: 1   MATEAPTQAPSEDLQSLSVSDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR 60
           MATEAP+QA SEDLQSLS+SDAKVQEPACSSN SNQLSLEEKYQIVRSVGEECIQEDELR
Sbjct: 1   MATEAPSQALSEDLQSLSISDAKVQEPACSSNFSNQLSLEEKYQIVRSVGEECIQEDELR 60

Query: 61  NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG 120
           NLLDKKPEPVCYDGFEPSGRMHIAQGVMK INVNKLTSAGCRVKIWIADWFAQLNNKMGG
Sbjct: 61  NLLDKKPEPVCYDGFEPSGRMHIAQGVMKTINVNKLTSAGCRVKIWIADWFAQLNNKMGG 120

Query: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPRI 180
           DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRA EYWPLVMDIAR+NKLPRI
Sbjct: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAAEYWPLVMDIARKNKLPRI 180

Query: 181 IRCSQIMGRNEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR 240
           IRCSQIMGRNEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR
Sbjct: 181 IRCSQIMGRNEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR 240

Query: 241 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL 300
           KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL
Sbjct: 241 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL 300

Query: 301 EYIKYLIFPWFKEFTVERNADHGGNKTFSTFEELVSDYAAGELHPGDLKPALSKALNKIL 360
           EYIKYLIFPWFKEFTVERNADHGGNKTF+TFEELV+DYA G LHPGDLKPALSKALNKIL
Sbjct: 301 EYIKYLIFPWFKEFTVERNADHGGNKTFATFEELVADYATGVLHPGDLKPALSKALNKIL 360

BLAST of CsGy5G002630 vs. NCBI nr
Match: XP_022969946.1 (tyrosine--tRNA ligase 1, cytoplasmic isoform X1 [Cucurbita maxima])

HSP 1 Score: 704 bits (1816), Expect = 6.01e-254
Identity = 347/380 (91.32%), Postives = 364/380 (95.79%), Query Frame = 0

Query: 1   MATEAPTQAPSEDLQSLSVSDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR 60
           MAT  P+QAPS+DLQSLS++DAKVQEP  SSNSSNQLSLEEK++IVRS+GEECIQEDELR
Sbjct: 1   MATGEPSQAPSQDLQSLSIADAKVQEPGSSSNSSNQLSLEEKFKIVRSIGEECIQEDELR 60

Query: 61  NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG 120
           NLL+KKPEPVCYDGFEPSGRMHIAQGVMK INVNKLT++GCRVKIWIADWFAQLNNKMGG
Sbjct: 61  NLLEKKPEPVCYDGFEPSGRMHIAQGVMKTINVNKLTASGCRVKIWIADWFAQLNNKMGG 120

Query: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPRI 180
           DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRA EYWPLVMDIAR+NKLPRI
Sbjct: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARKNKLPRI 180

Query: 181 IRCSQIMGRNEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR 240
           IRCSQIMGR EQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR
Sbjct: 181 IRCSQIMGRTEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR 240

Query: 241 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL 300
           KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL
Sbjct: 241 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL 300

Query: 301 EYIKYLIFPWFKEFTVERNADHGGNKTFSTFEELVSDYAAGELHPGDLKPALSKALNKIL 360
           EYIKYLIFPWFKEFTVERNADHGGNKTF+TFEELV+DYA+G LHPGDLKPALSKALNKIL
Sbjct: 301 EYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVADYASGVLHPGDLKPALSKALNKIL 360

Query: 361 EVC-HFCTILFHEYIPLLEL 379
           EVC HF +I  H  +  L+L
Sbjct: 361 EVCFHFYSISSHTPLCFLDL 380

BLAST of CsGy5G002630 vs. ExPASy TrEMBL
Match: A0A0A0KMK0 (Tyrosine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_5G153090 PE=3 SV=1)

HSP 1 Score: 731 bits (1886), Expect = 4.16e-265
Identity = 361/361 (100.00%), Postives = 361/361 (100.00%), Query Frame = 0

Query: 1   MATEAPTQAPSEDLQSLSVSDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR 60
           MATEAPTQAPSEDLQSLSVSDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR
Sbjct: 1   MATEAPTQAPSEDLQSLSVSDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR 60

Query: 61  NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG 120
           NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG
Sbjct: 61  NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG 120

Query: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPRI 180
           DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPRI
Sbjct: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPRI 180

Query: 181 IRCSQIMGRNEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR 240
           IRCSQIMGRNEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR
Sbjct: 181 IRCSQIMGRNEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR 240

Query: 241 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL 300
           KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL
Sbjct: 241 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL 300

Query: 301 EYIKYLIFPWFKEFTVERNADHGGNKTFSTFEELVSDYAAGELHPGDLKPALSKALNKIL 360
           EYIKYLIFPWFKEFTVERNADHGGNKTFSTFEELVSDYAAGELHPGDLKPALSKALNKIL
Sbjct: 301 EYIKYLIFPWFKEFTVERNADHGGNKTFSTFEELVSDYAAGELHPGDLKPALSKALNKIL 360

BLAST of CsGy5G002630 vs. ExPASy TrEMBL
Match: A0A1S3ATS8 (Tyrosine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103482753 PE=3 SV=1)

HSP 1 Score: 725 bits (1872), Expect = 5.66e-263
Identity = 357/361 (98.89%), Postives = 360/361 (99.72%), Query Frame = 0

Query: 1   MATEAPTQAPSEDLQSLSVSDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR 60
           MATEAP+QAPSEDLQSLS+SDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR
Sbjct: 1   MATEAPSQAPSEDLQSLSISDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR 60

Query: 61  NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG 120
           NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG
Sbjct: 61  NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG 120

Query: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPRI 180
           DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPRI
Sbjct: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPRI 180

Query: 181 IRCSQIMGRNEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR 240
           IRCSQIMGRNEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR
Sbjct: 181 IRCSQIMGRNEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR 240

Query: 241 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL 300
           KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVE NPCL
Sbjct: 241 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEANPCL 300

Query: 301 EYIKYLIFPWFKEFTVERNADHGGNKTFSTFEELVSDYAAGELHPGDLKPALSKALNKIL 360
           EYIKYLIFPWFKEFTVERNADHGGNKTF+TFEELVSDYAAGELHPGDLKPALSKALNKIL
Sbjct: 301 EYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVSDYAAGELHPGDLKPALSKALNKIL 360

BLAST of CsGy5G002630 vs. ExPASy TrEMBL
Match: A0A5A7THE1 (Tyrosine--tRNA ligase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002540 PE=3 SV=1)

HSP 1 Score: 725 bits (1872), Expect = 2.94e-262
Identity = 357/361 (98.89%), Postives = 360/361 (99.72%), Query Frame = 0

Query: 1   MATEAPTQAPSEDLQSLSVSDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR 60
           MATEAP+QAPSEDLQSLS+SDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR
Sbjct: 43  MATEAPSQAPSEDLQSLSISDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR 102

Query: 61  NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG 120
           NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG
Sbjct: 103 NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG 162

Query: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPRI 180
           DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPRI
Sbjct: 163 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPRI 222

Query: 181 IRCSQIMGRNEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR 240
           IRCSQIMGRNEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR
Sbjct: 223 IRCSQIMGRNEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR 282

Query: 241 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL 300
           KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVE NPCL
Sbjct: 283 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEANPCL 342

Query: 301 EYIKYLIFPWFKEFTVERNADHGGNKTFSTFEELVSDYAAGELHPGDLKPALSKALNKIL 360
           EYIKYLIFPWFKEFTVERNADHGGNKTF+TFEELVSDYAAGELHPGDLKPALSKALNKIL
Sbjct: 343 EYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVSDYAAGELHPGDLKPALSKALNKIL 402

BLAST of CsGy5G002630 vs. ExPASy TrEMBL
Match: A0A6J1HZ72 (Tyrosine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111468992 PE=3 SV=1)

HSP 1 Score: 704 bits (1816), Expect = 2.91e-254
Identity = 347/380 (91.32%), Postives = 364/380 (95.79%), Query Frame = 0

Query: 1   MATEAPTQAPSEDLQSLSVSDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR 60
           MAT  P+QAPS+DLQSLS++DAKVQEP  SSNSSNQLSLEEK++IVRS+GEECIQEDELR
Sbjct: 1   MATGEPSQAPSQDLQSLSIADAKVQEPGSSSNSSNQLSLEEKFKIVRSIGEECIQEDELR 60

Query: 61  NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG 120
           NLL+KKPEPVCYDGFEPSGRMHIAQGVMK INVNKLT++GCRVKIWIADWFAQLNNKMGG
Sbjct: 61  NLLEKKPEPVCYDGFEPSGRMHIAQGVMKTINVNKLTASGCRVKIWIADWFAQLNNKMGG 120

Query: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPRI 180
           DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRA EYWPLVMDIAR+NKLPRI
Sbjct: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARKNKLPRI 180

Query: 181 IRCSQIMGRNEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR 240
           IRCSQIMGR EQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR
Sbjct: 181 IRCSQIMGRTEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR 240

Query: 241 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL 300
           KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL
Sbjct: 241 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL 300

Query: 301 EYIKYLIFPWFKEFTVERNADHGGNKTFSTFEELVSDYAAGELHPGDLKPALSKALNKIL 360
           EYIKYLIFPWFKEFTVERNADHGGNKTF+TFEELV+DYA+G LHPGDLKPALSKALNKIL
Sbjct: 301 EYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVADYASGVLHPGDLKPALSKALNKIL 360

Query: 361 EVC-HFCTILFHEYIPLLEL 379
           EVC HF +I  H  +  L+L
Sbjct: 361 EVCFHFYSISSHTPLCFLDL 380

BLAST of CsGy5G002630 vs. ExPASy TrEMBL
Match: A0A6J1ER79 (Tyrosine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111435806 PE=3 SV=1)

HSP 1 Score: 704 bits (1816), Expect = 2.91e-254
Identity = 347/380 (91.32%), Postives = 367/380 (96.58%), Query Frame = 0

Query: 1   MATEAPTQAPSEDLQSLSVSDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR 60
           MATEAP+QAPS+DLQSLS+++AKVQEP  SS+SSNQLSLEEK++IVRS+GEECIQEDELR
Sbjct: 1   MATEAPSQAPSQDLQSLSIAEAKVQEPGSSSSSSNQLSLEEKFKIVRSIGEECIQEDELR 60

Query: 61  NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG 120
           NLL+KKPEPVCYDGFEPSGRMHIAQGVMK INVNKLT++GCRVKIWIADWFAQLNNKMGG
Sbjct: 61  NLLEKKPEPVCYDGFEPSGRMHIAQGVMKTINVNKLTASGCRVKIWIADWFAQLNNKMGG 120

Query: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPRI 180
           DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRA EYWPLVMDIAR+NKLPRI
Sbjct: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARKNKLPRI 180

Query: 181 IRCSQIMGRNEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR 240
           IRCSQIMGR EQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR
Sbjct: 181 IRCSQIMGRTEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR 240

Query: 241 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL 300
           KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL
Sbjct: 241 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL 300

Query: 301 EYIKYLIFPWFKEFTVERNADHGGNKTFSTFEELVSDYAAGELHPGDLKPALSKALNKIL 360
           EYIKYLIFPWFKEFTVERNADHGGNKTF+TFEELV+DYA+G LHPGDLKPALSKALNKIL
Sbjct: 301 EYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVADYASGVLHPGDLKPALSKALNKIL 360

Query: 361 EVC-HFCTILFHEYIPLLEL 379
           EVC HF +I  +  + LL+L
Sbjct: 361 EVCSHFYSISSNTPLCLLDL 380

BLAST of CsGy5G002630 vs. TAIR 10
Match: AT2G33840.1 (Tyrosyl-tRNA synthetase, class Ib, bacterial/mitochondrial )

HSP 1 Score: 569.3 bits (1466), Expect = 2.5e-162
Identity = 272/355 (76.62%), Postives = 312/355 (87.89%), Query Frame = 0

Query: 9   APSEDLQSLSVSDAKVQEPACSSNSSNQLS--LEEKYQIVRSVGEECIQEDELRNLLDKK 68
           A S +++++SVS  +    A SS+   Q+S  +E++Y+IVRS+GEECIQE+EL+NLL KK
Sbjct: 11  ALSSEMEAVSVSSTQ----ASSSSDGFQMSEEVEKRYKIVRSIGEECIQEEELKNLLAKK 70

Query: 69  PEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGGDLKKIE 128
             P+CYDGFEPSGRMHIAQGVMK INVNK+TSAGCRVKIWIADWFAQLNNKMGGDLKKI 
Sbjct: 71  AAPICYDGFEPSGRMHIAQGVMKVINVNKMTSAGCRVKIWIADWFAQLNNKMGGDLKKIR 130

Query: 129 TVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPRIIRCSQI 188
            VG Y  EIWKA GMD    KVEFLWSS+EINS+A +YWPLVMDIAR+NKLPRI+RC QI
Sbjct: 131 VVGEYFQEIWKAAGMD--NDKVEFLWSSEEINSKADKYWPLVMDIARKNKLPRILRCVQI 190

Query: 189 MGRNEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKRKNKPII 248
           MGR+E DEL+AAQILYPCMQCADIFFL+ADICQLGMDQRKVNVLAREYCDDIKRKNKPII
Sbjct: 191 MGRSETDELSAAQILYPCMQCADIFFLEADICQLGMDQRKVNVLAREYCDDIKRKNKPII 250

Query: 249 LSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCLEYIKYL 308
           LSHHMLPGLQQGQEKMSKSDP S+IFMED+EAEVN+KIKKAYCPP +V+GNPCLEYIKY+
Sbjct: 251 LSHHMLPGLQQGQEKMSKSDPLSAIFMEDEEAEVNVKIKKAYCPPKVVKGNPCLEYIKYI 310

Query: 309 IFPWFKEFTVERNADHGGNKTFSTFEELVSDYAAGELHPGDLKPALSKALNKILE 362
           I PWF EFTVERN ++GGNKT+ +FE++ +DY +GELHPGDLK  L  ALNKIL+
Sbjct: 311 ILPWFDEFTVERNEEYGGNKTYKSFEDIAADYESGELHPGDLKKGLMNALNKILQ 359

BLAST of CsGy5G002630 vs. TAIR 10
Match: AT1G28350.1 (Nucleotidylyl transferase superfamily protein )

HSP 1 Score: 519.2 bits (1336), Expect = 2.9e-147
Identity = 252/348 (72.41%), Postives = 288/348 (82.76%), Query Frame = 0

Query: 16  SLSVSDAKVQEPACSSNSSNQLS--LEEKYQIVRSVGEECIQEDELRNLLDKKPEPVCYD 75
           S    D  +   A SS +  Q+S   E KY+IVRS+GEECIQEDEL+NLL KKP P+CYD
Sbjct: 378 STKEEDLSINTSASSSAAGLQMSEEAEMKYKIVRSIGEECIQEDELKNLLAKKPAPICYD 437

Query: 76  GFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGGDLKKIETVGRYLI 135
           GFEPSGRMHIAQGVMK  NVNKLTSAGC+VKIWIADWFAQLNNK+GGDL++I  VG Y  
Sbjct: 438 GFEPSGRMHIAQGVMKVTNVNKLTSAGCQVKIWIADWFAQLNNKLGGDLERIRVVGEYFK 497

Query: 136 EIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPRIIRCSQIMGRNEQD 195
           EIW+A GM+    KVEFLW+S EIN +  +YWPLVMDIARRN L RI+RC QIMGR+E +
Sbjct: 498 EIWQAGGMN--NDKVEFLWASDEINGKGSKYWPLVMDIARRNNLRRILRCGQIMGRSETE 557

Query: 196 ELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKRKNKPIILSHHMLP 255
            L+AAQILYPCMQCADIF L+ADICQLGMDQRKVN+LAREYC DIKRKNKPIILSHHMLP
Sbjct: 558 VLSAAQILYPCMQCADIFLLEADICQLGMDQRKVNMLAREYCADIKRKNKPIILSHHMLP 617

Query: 256 GLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCLEYIKYLIFPWFKE 315
           GLQQGQEKMSKSDPSS+IFMED+EA+VN KI KAYCPP  VEGNPCLEY+KY++ P F E
Sbjct: 618 GLQQGQEKMSKSDPSSAIFMEDEEADVNEKISKAYCPPKTVEGNPCLEYVKYIVLPRFNE 677

Query: 316 FTVERNADHGGNKTFSTFEELVSDYAAGELHPGDLKPALSKALNKILE 362
           F VE +  +GGNKTF++FE++V+DY  GELHP DLK AL KALN  L+
Sbjct: 678 FKVE-SEKNGGNKTFNSFEDIVADYETGELHPEDLKKALMKALNITLQ 722

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8S9J23.5e-16176.62Tyrosine--tRNA ligase 1, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At2g3384... [more]
F4HWL44.1e-14672.41Tyrosine--tRNA ligase 2, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At1g2835... [more]
Q5UPJ73.1e-6943.87Tyrosine--tRNA ligase OS=Acanthamoeba polyphaga mimivirus OX=212035 GN=YARS PE=1... [more]
Q5JF631.7e-5937.01Tyrosine--tRNA ligase OS=Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12... [more]
Q8U2H32.9e-5935.59Tyrosine--tRNA ligase OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM... [more]
Match NameE-valueIdentityDescription
XP_004143904.18.59e-265100.00tyrosine--tRNA ligase 1, cytoplasmic [Cucumis sativus] >KGN50094.1 hypothetical ... [more]
XP_008437283.11.17e-26298.89PREDICTED: tyrosine--tRNA ligase 1, cytoplasmic [Cucumis melo][more]
KAA0042754.16.07e-26298.89tyrosine--tRNA ligase 1 [Cucumis melo var. makuwa][more]
XP_038906448.15.07e-25796.95tyrosine--tRNA ligase 1, cytoplasmic [Benincasa hispida][more]
XP_022969946.16.01e-25491.32tyrosine--tRNA ligase 1, cytoplasmic isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A0A0KMK04.16e-265100.00Tyrosine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_5G153090 PE=3 SV=1[more]
A0A1S3ATS85.66e-26398.89Tyrosine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103482753 PE=3 SV=1[more]
A0A5A7THE12.94e-26298.89Tyrosine--tRNA ligase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A6J1HZ722.91e-25491.32Tyrosine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111468992 PE=3 SV=1[more]
A0A6J1ER792.91e-25491.32Tyrosine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111435806 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G33840.12.5e-16276.62Tyrosyl-tRNA synthetase, class Ib, bacterial/mitochondrial [more]
AT1G28350.12.9e-14772.41Nucleotidylyl transferase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 199..365
e-value: 5.3E-71
score: 241.2
coord: 30..198
e-value: 3.6E-69
score: 235.2
IPR002305Aminoacyl-tRNA synthetase, class IcPFAMPF00579tRNA-synt_1bcoord: 67..361
e-value: 3.6E-65
score: 220.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..35
NoneNo IPR availablePANTHERPTHR46264:SF8BNAANNG11300D PROTEINcoord: 27..365
NoneNo IPR availablePANTHERPTHR46264TYROSINE-TRNA LIGASEcoord: 27..365
NoneNo IPR availableSUPERFAMILY52374Nucleotidylyl transferasecoord: 43..361

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy5G002630.2CsGy5G002630.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006418 tRNA aminoacylation for protein translation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004831 tyrosine-tRNA ligase activity
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0000166 nucleotide binding