CsGy5G002410 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy5G002410
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionBeta-carotene hydroxylase
LocationGy14Chr5: 1523998 .. 1527274 (+)
RNA-Seq ExpressionCsGy5G002410
SyntenyCsGy5G002410
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTTGCCTTAAATAATATAAGAAGTGTAAGGTTATTTTAGTAACTTAACCCTTATACCAAAGGTGACAATTCTTCTTTAACCAATTGGCTACGCAGCCTCCAAGAAACTCAAACTTCAATTTAAATCGATCCTAATTTTTCATAACTCTCGAATTCCATTTCCTTTCATGACGACGGTGATTTCGGCCGTTGCAACCGCCGGGCCGTACCGGTATGATTACGGGAGGTTGTTTCAACGCCCGGCCGCCGGAGCTCCGAGTCCAGGTTTTCCATGGCGGTGTTTTGCGAAACAGAGGTGGAGAAGGAGAAGATTGATGGTTGTGAAGATGAAGAAGAAGAAAAGTGAAGAAGATGAAATGGTTAAGGAATTGAAAATGAGTGTGGAGGAGAAAATGGGGAAGAAGAAAGCAGAAAGAGAGGGTTATTTGATAGCTGCAATTGTTTCGAGCTTTGGGATCACTTCAATGGCCGCCATCGCTGTTTATTACCGTTTCTCTTCCCAAATAATGGAGGTACTCTGTTCTCATTAGCGTATATGTGTTAAAATAAAACTAATCGATTCTATTAAATTATTTTATATCTAACTTATAAATTAATACTTGGTGTCATCCATAGATTTCACTCTACATTCCAGCTCCCCTCTCTTCGTCGTCTCTATTATATACACATCAATATCATTAGTTGATTGAGTAGTAAATTAGTTTTTCATATCCTCTAAACTCCAATCAAATTCTATACTTTACATTTTTATTCATATTGTATTTTTACTTATACAATCCCATCAACAAAATTTACATATAGATTTGTGATTTGTGGTTGTTTGATGCATCTAATCATTTTGTAGCTAAATTACTTTTTTCTAAAAGCGGATGTATTTCGACTTTTAGGTGAGTTGCTAATTAAAAGAGATTTAAAAAAATTAAAATAAAAATGAGATCATGAAGTGTGTGAAACCATAAAAAAGAGAGTTTGATTGAAAATAGTTGTAACAAAATATTCAATCAAAACATGAATTTTAAATTACATTACATTACAAACTCATTTCATTACAATTCACTAAACTTCTAAAATATCCTCACTCATTATGTATTATTTAAACAATTTATCGAGACGTTTTTATTGAAACACGAATAATATACACGTTTAGTTGGAAAATGAATCTATATAATGTATGTAATGTGACATATTTTCATATAAAAAAGAAGTGGTATGCAATAAATGTGGTAAGAAATCTTTTTTAGCATTATGTTGGTATATACACTACCATTTCTATTTACAATTTGACAAGTTATGTGTTAATGACAAGTTTGAGAATTTTGCGTTCTGTTTTGGGACTTTCTCTTCTTTCCTCTTTCAATAATACCATGGATTTTCCTCTTTTCTTCTGCTTCATCTTTTTGTTTCTGCTTTTACTTTCATCCTATGCTTTTATATATATATATATATTGGGATCTAACCCTTTTAAAGTGTTTTTAGGGTATGAAATTTTTATTATTACTCATGTATTCAAATACATTAACTAACTAAGTGATATAAAGAAATAGTTTGAAAATTTTAAATTGAAGATAAATTTATTTATGTTTTCGATAAAAAGAAAAGATATGAGTTTTATTATCTATTTTGCAGGGCGGAGAATTTCCACTTTTAGAAATGTTCGGCACTTTTGTACTATCTATTGGTTCTGCTGTAAGTTACGTTATTTAAATTTCAAATGAACTAAAAGAGATCGTTTGAAACATTTAAAAAGTCATTTCGACCAATTCTGAAAACATTTTTCACTGATGACAGGTGGGCATGGAATTTTGGGCGAGATGGGCTCACAAAGAGCTATGGCATGCTTCGTTATGGGATATGCATGAGGTAATTAATAATATAACGAAGGTGTTACATACAGAATATAATAAAGTTAAAAGAAGCATATAGTAAAAGTTGTTATTATTGGGTAGTCTCATCACAAGCCGAGGGTTGGAGCATTTGAAAAGAACGATGTGTTTGCAATTATAAACGCGATTCCAGCCATTGCTCTTCTTTCTTTCGGCCTCTTCAATCAAGGCTTCTTTCCCGGCCTTTGTTTTGGTGCCGTACGTCTCTTCTTTCTCTCCTCTTTTTTTACTTTTTTTTTTTTCAATTATACTTTACTCATAGTTCATTCCATTTTTTTCTTAAATTTCAAACACTTGTTTTGTTTTTGTTTTTGTTAAGAATTGTGAAAAAATATATTAAATAAAATAGAAATTTCAAAACCAGAAAGCAACCAACAGAATTTCGACTGAATCAAACCTAAAGATTGCTTCCATCTAAATCCAGAAATAAATTTGTAATTTATCAAATAGAGTATGAAGTAGATTAGATCTTTTTCTTTTTCTTAGAAATATGAAGCAATATTTAAAGTATTTCCTACTAATTTTATGAATATTGATGGATGATTCATACTTTCCAACTTTAGAATCATTCCAACCTAACTTAGCAAAGTGGTGAAGGAAAATGAAGTCCAAGTCAGCTTTTTGTATGACGTGGCTTTCCACGATTGGCAATTGTACAGGGTTTGGGAATTACGGTGTTTGGGATGGCCTACATGTTCGTCCACGATGGCCTCGTTCACCGTCGTTTCCCCGTGGGTCCGATAGCCGCTGTCCCCTACTTACGACGTGTCGCTGCTGCTCATCATGTACTTTTCGACTCGTCGTCCCTCTTGTCTATTTATTTTTAATATTTAATACACTTTCAGAAACATTTTAAAAAATAGTAAATTTGATTAAAAAAATTAAAAAAATTATCAGTTATATTCTTATAAAATTTAAGAGTTTTGTTATAACTTATAAATATTTTAATTTATTTTGTTATTTTTAAAAATATTTATATTTTTAAAAATTTTGTTTATATAATTTTGTGCAGATCCATCATACGGATAAATTTGACGGAGTTCCATATGGGTTGTTTTTAGGACCCAAGGTTAGTCTCGTCATTTTGTATGCTAAGTATGATTTCAAAGGCTGTATTGTTCATTCCCTGCCAATATTTTCAATTTTTATTTGGAAACATATTCACTATATTGCATATTCTTACACTTTGCTATTATTATTACTATTGAATTTGAAATTTATTAAAAGAATATATACTAATTGTCTTAAACGACGGCGTTCGTCTTCAGGAACTGGAAGAAGTGGATGGCGAGGAAGAGCTGCAGAAGGAAATTAGGAGGAGAAAGGTTTACAGAAATTAAATTCAATTTTCTTATAATGTTATAATTATTTTTATAAAAATTACAACAAGTAGT

mRNA sequence

CCTTGCCTTAAATAATATAAGAAGTGTAAGGTTATTTTAGTAACTTAACCCTTATACCAAAGGTGACAATTCTTCTTTAACCAATTGGCTACGCAGCCTCCAAGAAACTCAAACTTCAATTTAAATCGATCCTAATTTTTCATAACTCTCGAATTCCATTTCCTTTCATGACGACGGTGATTTCGGCCGTTGCAACCGCCGGGCCGTACCGGTATGATTACGGGAGGTTGTTTCAACGCCCGGCCGCCGGAGCTCCGAGTCCAGGTTTTCCATGGCGGTGTTTTGCGAAACAGAGGTGGAGAAGGAGAAGATTGATGGTTGTGAAGATGAAGAAGAAGAAAAGTGAAGAAGATGAAATGGTTAAGGAATTGAAAATGAGTGTGGAGGAGAAAATGGGGAAGAAGAAAGCAGAAAGAGAGGGTTATTTGATAGCTGCAATTGTTTCGAGCTTTGGGATCACTTCAATGGCCGCCATCGCTGTTTATTACCGTTTCTCTTCCCAAATAATGGAGGGCGGAGAATTTCCACTTTTAGAAATGTTCGGCACTTTTGTACTATCTATTGGTTCTGCTGTGGGCATGGAATTTTGGGCGAGATGGGCTCACAAAGAGCTATGGCATGCTTCGTTATGGGATATGCATGAGTCTCATCACAAGCCGAGGGTTGGAGCATTTGAAAAGAACGATGTGTTTGCAATTATAAACGCGATTCCAGCCATTGCTCTTCTTTCTTTCGGCCTCTTCAATCAAGGCTTCTTTCCCGGCCTTTGTTTTGGTGCCGGTTTGGGAATTACGGTGTTTGGGATGGCCTACATGTTCGTCCACGATGGCCTCGTTCACCGTCGTTTCCCCGTGGGTCCGATAGCCGCTGTCCCCTACTTACGACGTGTCGCTGCTGCTCATCATATCCATCATACGGATAAATTTGACGGAGTTCCATATGGGTTGTTTTTAGGACCCAAGGAACTGGAAGAAGTGGATGGCGAGGAAGAGCTGCAGAAGGAAATTAGGAGGAGAAAGGTTTACAGAAATTAAATTCAATTTTCTTATAATGTTATAATTATTTTTATAAAAATTACAACAAGTAGT

Coding sequence (CDS)

ATGACGACGGTGATTTCGGCCGTTGCAACCGCCGGGCCGTACCGGTATGATTACGGGAGGTTGTTTCAACGCCCGGCCGCCGGAGCTCCGAGTCCAGGTTTTCCATGGCGGTGTTTTGCGAAACAGAGGTGGAGAAGGAGAAGATTGATGGTTGTGAAGATGAAGAAGAAGAAAAGTGAAGAAGATGAAATGGTTAAGGAATTGAAAATGAGTGTGGAGGAGAAAATGGGGAAGAAGAAAGCAGAAAGAGAGGGTTATTTGATAGCTGCAATTGTTTCGAGCTTTGGGATCACTTCAATGGCCGCCATCGCTGTTTATTACCGTTTCTCTTCCCAAATAATGGAGGGCGGAGAATTTCCACTTTTAGAAATGTTCGGCACTTTTGTACTATCTATTGGTTCTGCTGTGGGCATGGAATTTTGGGCGAGATGGGCTCACAAAGAGCTATGGCATGCTTCGTTATGGGATATGCATGAGTCTCATCACAAGCCGAGGGTTGGAGCATTTGAAAAGAACGATGTGTTTGCAATTATAAACGCGATTCCAGCCATTGCTCTTCTTTCTTTCGGCCTCTTCAATCAAGGCTTCTTTCCCGGCCTTTGTTTTGGTGCCGGTTTGGGAATTACGGTGTTTGGGATGGCCTACATGTTCGTCCACGATGGCCTCGTTCACCGTCGTTTCCCCGTGGGTCCGATAGCCGCTGTCCCCTACTTACGACGTGTCGCTGCTGCTCATCATATCCATCATACGGATAAATTTGACGGAGTTCCATATGGGTTGTTTTTAGGACCCAAGGAACTGGAAGAAGTGGATGGCGAGGAAGAGCTGCAGAAGGAAATTAGGAGGAGAAAGGTTTACAGAAATTAA

Protein sequence

MTTVISAVATAGPYRYDYGRLFQRPAAGAPSPGFPWRCFAKQRWRRRRLMVVKMKKKKSEEDEMVKELKMSVEEKMGKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIMEGGEFPLLEMFGTFVLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINAIPAIALLSFGLFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIRRRKVYRN*
Homology
BLAST of CsGy5G002410 vs. ExPASy Swiss-Prot
Match: Q9SZZ8 (Beta-carotene 3-hydroxylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BETA-OHASE 1 PE=1 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 2.0e-94
Identity = 168/216 (77.78%), Postives = 189/216 (87.50%), Query Frame = 0

Query: 68  LKMSVEEKMGKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIMEGGEFPLLEMFGT 127
           L + + EK+ +KK+ER  YLIAA++SSFGITSMA +AVYYRFS Q MEGGE  +LEMFGT
Sbjct: 80  LALRLAEKLERKKSERSTYLIAAMLSSFGITSMAVMAVYYRFSWQ-MEGGEISMLEMFGT 139

Query: 128 FVLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINAIPAIALL 187
           F LS+G+AVGMEFWARWAH+ LWHASLW+MHESHHKPR G FE NDVFAI+NA PAI LL
Sbjct: 140 FALSVGAAVGMEFWARWAHRALWHASLWNMHESHHKPREGPFELNDVFAIVNAGPAIGLL 199

Query: 188 SFGLFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHI 247
           S+G FN+G  PGLCFGAGLGITVFG+AYMFVHDGLVH+RFPVGPIA VPYLR+VAAAH +
Sbjct: 200 SYGFFNKGLVPGLCFGAGLGITVFGIAYMFVHDGLVHKRFPVGPIADVPYLRKVAAAHQL 259

Query: 248 HHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIRRR 284
           HHTDKF+GVPYGLFLGPKELEEV G EEL KEI RR
Sbjct: 260 HHTDKFNGVPYGLFLGPKELEEVGGNEELDKEISRR 294

BLAST of CsGy5G002410 vs. ExPASy Swiss-Prot
Match: O49814 (Beta-carotene hydroxylase 2, chloroplastic OS=Capsicum annuum OX=4072 GN=CA2 PE=1 SV=2)

HSP 1 Score: 340.5 bits (872), Expect = 1.9e-92
Identity = 172/238 (72.27%), Postives = 199/238 (83.61%), Query Frame = 0

Query: 53  KMKKKKSEED-EMVKELKMS---VEEKMGKKKAEREGYLIAAIVSSFGITSMAAIAVYYR 112
           K + +  EED EM  E ++S   + EK+ +KK+ER  YL+AA++SSFGITSMA +AVYYR
Sbjct: 68  KTQFEAGEEDIEMKIEEQISATRLAEKLARKKSERFTYLVAAVMSSFGITSMAVMAVYYR 127

Query: 113 FSSQIMEGGEFPLLEMFGTFVLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGA 172
           F  Q MEGGE P  EMFGTF LS+G+AVGMEFWARWAHK LWHASLW MHESHHKPR G 
Sbjct: 128 FYWQ-MEGGEVPFSEMFGTFALSVGAAVGMEFWARWAHKALWHASLWHMHESHHKPREGP 187

Query: 173 FEKNDVFAIINAIPAIALLSFGLFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFP 232
           FE NDVFAIINA+PAIALL +G F++G  PGLCFGAGLGITVFGMAYMFVHDGLVH+RFP
Sbjct: 188 FELNDVFAIINAVPAIALLDYGFFHKGLIPGLCFGAGLGITVFGMAYMFVHDGLVHKRFP 247

Query: 233 VGPIAAVPYLRRVAAAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIRRRKVY 287
           VGP+A VPYLR+VAAAH +HH++KF+GVPYGLFLGPKELEEV G EEL+KE+ RR  Y
Sbjct: 248 VGPVANVPYLRKVAAAHSLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEVNRRTRY 304

BLAST of CsGy5G002410 vs. ExPASy Swiss-Prot
Match: B3SGL0 (Beta-carotene 3-hydroxylase, chloroplastic OS=Gentiana lutea OX=38851 GN=BHY PE=2 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 1.9e-92
Identity = 166/221 (75.11%), Postives = 190/221 (85.97%), Query Frame = 0

Query: 66  KELKMSVE---EKMGKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIMEGGEFPLL 125
           KE+  S E   +K+ +KK+ER  YL+AA++SSFGITSMA ++VYYRFS Q MEGGE PL 
Sbjct: 95  KEISASAEKLAQKLARKKSERFTYLVAAVMSSFGITSMAVLSVYYRFSWQ-MEGGEIPLS 154

Query: 126 EMFGTFVLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINAIP 185
           EMFGTF LS+G+AVGMEFWARWAH+ LWHASLW MHESHHKPR G FE ND+FAIINA+P
Sbjct: 155 EMFGTFALSVGAAVGMEFWARWAHEALWHASLWHMHESHHKPREGPFELNDIFAIINAVP 214

Query: 186 AIALLSFGLFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVA 245
           AIALLS+G F++G  PGLCFGAGLGITVFGMAYMFVHDGLVH+RFPVGPIA VPY RRVA
Sbjct: 215 AIALLSYGFFHKGLIPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIADVPYFRRVA 274

Query: 246 AAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIRRR 284
           AAH +HH+DKF+GVPYGLFLGPKELEEV G + L+ EI RR
Sbjct: 275 AAHTLHHSDKFNGVPYGLFLGPKELEEVGGLQVLEMEINRR 314

BLAST of CsGy5G002410 vs. ExPASy Swiss-Prot
Match: Q9LTG0 (Beta-carotene 3-hydroxylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BETA-OHASE 2 PE=2 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 1.3e-90
Identity = 176/258 (68.22%), Postives = 209/258 (81.01%), Query Frame = 0

Query: 44  WRRRRLM----VVKMKKKKSEEDEMVK--------ELKMS--VEEKMGKKKAEREGYLIA 103
           +RRR+++    VV+ +K+ S  D+  K        E+ M+  + +K  KKK+ER  YLIA
Sbjct: 40  FRRRKILTVCFVVEERKQSSPMDDDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIA 99

Query: 104 AIVSSFGITSMAAIAVYYRFSSQIMEGGEFPLLEMFGTFVLSIGSAVGMEFWARWAHKEL 163
           A++SSFGITSMA +AVYYRFS Q M+GGE  +LEMFGTF LS+G+AVGMEFWARWAH+ L
Sbjct: 100 AVMSSFGITSMAIMAVYYRFSWQ-MKGGEVSVLEMFGTFALSVGAAVGMEFWARWAHRAL 159

Query: 164 WHASLWDMHESHHKPRVGAFEKNDVFAIINAIPAIALLSFGLFNQGFFPGLCFGAGLGIT 223
           WH SLW+MHESHHKPR GAFE NDVFAI NA+PAI LL +G  N+G  PGLCFGAGLGIT
Sbjct: 160 WHDSLWNMHESHHKPREGAFELNDVFAITNAVPAIGLLYYGFLNKGLVPGLCFGAGLGIT 219

Query: 224 VFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHIHHTDKFDGVPYGLFLGPKELEE 283
           +FGMAYMFVHDGLVH+RFPVGPIA VPYLR+VAAAH +HHTDKF GVPYGLFLGPKE+EE
Sbjct: 220 MFGMAYMFVHDGLVHKRFPVGPIANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPKEVEE 279

Query: 284 VDGEEELQKEIRRR-KVY 287
           V G+EEL+KEI RR K+Y
Sbjct: 280 VGGKEELEKEISRRIKLY 296

BLAST of CsGy5G002410 vs. ExPASy Swiss-Prot
Match: O49815 (Beta-carotene hydroxylase 1, chloroplastic OS=Capsicum annuum OX=4072 GN=CA1 PE=1 SV=2)

HSP 1 Score: 331.6 bits (849), Expect = 8.6e-90
Identity = 160/218 (73.39%), Postives = 184/218 (84.40%), Query Frame = 0

Query: 66  KELKMSVEEKMGKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIMEGGEFPLLEMF 125
           K L + + EK  +KK+ER  YL+AA++SS GITSMA I+VYYRFS Q MEGGE P  EMF
Sbjct: 92  KSLAVRLAEKFARKKSERFTYLVAAVMSSLGITSMAVISVYYRFSWQ-MEGGEMPFSEMF 151

Query: 126 GTFVLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINAIPAIA 185
            TF L+ G+A+GME+WARWAH+ LWHASLW MHESHH+PR G FE ND+FAIINA+PAIA
Sbjct: 152 CTFALAFGAAIGMEYWARWAHRALWHASLWHMHESHHRPREGPFELNDIFAIINAVPAIA 211

Query: 186 LLSFGLFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAH 245
           LLSFG  ++G  PGLCFGAGLGITVFGMAYMFVHDGLVH+RFPVGPIA VPY +RVAAAH
Sbjct: 212 LLSFGFNHKGLIPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFQRVAAAH 271

Query: 246 HIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIRRR 284
            +HH+DKFDGVPYGLFLGPKELEEV   EEL+KE+ RR
Sbjct: 272 QLHHSDKFDGVPYGLFLGPKELEEVGVLEELEKEVNRR 308

BLAST of CsGy5G002410 vs. NCBI nr
Match: XP_004143973.1 (beta-carotene 3-hydroxylase 1, chloroplastic [Cucumis sativus] >KGN50067.1 hypothetical protein Csa_000162 [Cucumis sativus])

HSP 1 Score: 577 bits (1487), Expect = 1.25e-207
Identity = 288/288 (100.00%), Postives = 288/288 (100.00%), Query Frame = 0

Query: 1   MTTVISAVATAGPYRYDYGRLFQRPAAGAPSPGFPWRCFAKQRWRRRRLMVVKMKKKKSE 60
           MTTVISAVATAGPYRYDYGRLFQRPAAGAPSPGFPWRCFAKQRWRRRRLMVVKMKKKKSE
Sbjct: 1   MTTVISAVATAGPYRYDYGRLFQRPAAGAPSPGFPWRCFAKQRWRRRRLMVVKMKKKKSE 60

Query: 61  EDEMVKELKMSVEEKMGKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIMEGGEFP 120
           EDEMVKELKMSVEEKMGKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIMEGGEFP
Sbjct: 61  EDEMVKELKMSVEEKMGKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIMEGGEFP 120

Query: 121 LLEMFGTFVLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINA 180
           LLEMFGTFVLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINA
Sbjct: 121 LLEMFGTFVLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINA 180

Query: 181 IPAIALLSFGLFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRR 240
           IPAIALLSFGLFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRR
Sbjct: 181 IPAIALLSFGLFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRR 240

Query: 241 VAAAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIRRRKVYRN 288
           VAAAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIRRRKVYRN
Sbjct: 241 VAAAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIRRRKVYRN 288

BLAST of CsGy5G002410 vs. NCBI nr
Match: XP_008437325.1 (PREDICTED: beta-carotene hydroxylase 2, chloroplastic-like isoform X1 [Cucumis melo])

HSP 1 Score: 543 bits (1398), Expect = 4.12e-194
Identity = 275/288 (95.49%), Postives = 279/288 (96.88%), Query Frame = 0

Query: 1   MTTVISAVATAGPYRYDYGRLFQRPAAGAPSPGFPWRCFAKQRWRRRRLMVVKMKKKKSE 60
           M TVISAVATAG YRYDYGRLFQRPA GAPS GFPWRCFAKQRWRR  LMV+KMKKKKSE
Sbjct: 1   MATVISAVATAGQYRYDYGRLFQRPA-GAPSRGFPWRCFAKQRWRR--LMVMKMKKKKSE 60

Query: 61  EDEMVKELKMSVEEKMGKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIMEGGEFP 120
           EDEMVK+LKMSVEEKM KKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIMEGGEFP
Sbjct: 61  EDEMVKKLKMSVEEKMRKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIMEGGEFP 120

Query: 121 LLEMFGTFVLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINA 180
           +LEMFGTFVLSIGS+VGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINA
Sbjct: 121 ILEMFGTFVLSIGSSVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINA 180

Query: 181 IPAIALLSFGLFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRR 240
           IPAIALLSFGLFN GFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRR
Sbjct: 181 IPAIALLSFGLFNHGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRR 240

Query: 241 VAAAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIRRRKVYRN 288
           VAAAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIRRR VYRN
Sbjct: 241 VAAAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIRRRNVYRN 285

BLAST of CsGy5G002410 vs. NCBI nr
Match: XP_016898856.1 (PREDICTED: beta-carotene hydroxylase 2, chloroplastic-like isoform X3 [Cucumis melo])

HSP 1 Score: 493 bits (1270), Expect = 6.36e-175
Identity = 257/288 (89.24%), Postives = 259/288 (89.93%), Query Frame = 0

Query: 1   MTTVISAVATAGPYRYDYGRLFQRPAAGAPSPGFPWRCFAKQRWRRRRLMVVKMKKKKSE 60
           M TVISAVATAG YRYDYGRLFQRPA GAPS GFPWRCFAKQRWRR  LMV+KMKKKKSE
Sbjct: 1   MATVISAVATAGQYRYDYGRLFQRPA-GAPSRGFPWRCFAKQRWRR--LMVMKMKKKKSE 60

Query: 61  EDEMVKELKMSVEEKMGKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIMEGGEFP 120
           EDEMVK+LKMSVEEKM KKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIME     
Sbjct: 61  EDEMVKKLKMSVEEKMRKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIME----- 120

Query: 121 LLEMFGTFVLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINA 180
                          VGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINA
Sbjct: 121 ---------------VGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINA 180

Query: 181 IPAIALLSFGLFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRR 240
           IPAIALLSFGLFN GFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRR
Sbjct: 181 IPAIALLSFGLFNHGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRR 240

Query: 241 VAAAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIRRRKVYRN 288
           VAAAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIRRR VYRN
Sbjct: 241 VAAAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIRRRNVYRN 265

BLAST of CsGy5G002410 vs. NCBI nr
Match: XP_016898855.1 (PREDICTED: beta-carotene hydroxylase 2, chloroplastic-like isoform X2 [Cucumis melo])

HSP 1 Score: 488 bits (1256), Expect = 8.97e-173
Identity = 256/288 (88.89%), Postives = 260/288 (90.28%), Query Frame = 0

Query: 1   MTTVISAVATAGPYRYDYGRLFQRPAAGAPSPGFPWRCFAKQRWRRRRLMVVKMKKKKSE 60
           M TVISAVATAG YRYDYGRLFQRPA GAPS GFPWRCFAKQRWRR  LMV+KMKKKKSE
Sbjct: 1   MATVISAVATAGQYRYDYGRLFQRPA-GAPSRGFPWRCFAKQRWRR--LMVMKMKKKKSE 60

Query: 61  EDEMVKELKMSVEEKMGKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIMEGGEFP 120
           EDEMVK+LKMSVEEKM KKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIMEGGEFP
Sbjct: 61  EDEMVKKLKMSVEEKMRKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIMEGGEFP 120

Query: 121 LLEMFGTFVLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINA 180
           +LEMFGTFVLSIGS+VGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINA
Sbjct: 121 ILEMFGTFVLSIGSSVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINA 180

Query: 181 IPAIALLSFGLFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRR 240
           IPAIALLSFGLFN GFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRR
Sbjct: 181 IPAIALLSFGLFNHGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRR 240

Query: 241 VAAAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIRRRKVYRN 288
           VAAAHH                   ELEEVDGEEELQKEIRRR VYRN
Sbjct: 241 VAAAHH-------------------ELEEVDGEEELQKEIRRRNVYRN 266

BLAST of CsGy5G002410 vs. NCBI nr
Match: XP_038907152.1 (beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X1 [Benincasa hispida])

HSP 1 Score: 461 bits (1185), Expect = 1.21e-161
Identity = 241/295 (81.69%), Postives = 258/295 (87.46%), Query Frame = 0

Query: 1   MTTVISAVATAGPYRYDYGRLFQRPAAGAPSP------GFPWRCFAKQRWRRRRLMVVKM 60
           M TVI+AVATA P+     R F RPA GAP P       FPWRCFA+QR RR    ++ M
Sbjct: 1   MATVIAAVATAAPFNQ---RFFHRPA-GAPPPRLGFSNSFPWRCFAEQRSRR----LLAM 60

Query: 61  KKKKSEEDEMVKELKMSVEEKMGKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIM 120
           KKKKSEEDEMVKELKMSV++KMG+KKAER+ YL+AAIVSS G TSMAAIAVYYRFS Q M
Sbjct: 61  KKKKSEEDEMVKELKMSVDKKMGRKKAERDAYLVAAIVSSIGFTSMAAIAVYYRFSWQ-M 120

Query: 121 EGGEFPLLEMFGTFVLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDV 180
           EG E P+ EMFGTF LSIG+AVGMEFWARWAHKELWH SLWDMHESHH+PRVGAFE NDV
Sbjct: 121 EGREIPIWEMFGTFALSIGTAVGMEFWARWAHKELWHGSLWDMHESHHRPRVGAFELNDV 180

Query: 181 FAIINAIPAIALLSFGLFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAA 240
           FAIINA+PAI+LLSFG FNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFP+GPIAA
Sbjct: 181 FAIINAVPAISLLSFGFFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPLGPIAA 240

Query: 241 VPYLRRVAAAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIRRR-KVYRN 288
           VPYLRRVAAAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEI+RR KVYRN
Sbjct: 241 VPYLRRVAAAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIKRRIKVYRN 286

BLAST of CsGy5G002410 vs. ExPASy TrEMBL
Match: A0A0A0KNU9 (Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G152820 PE=3 SV=1)

HSP 1 Score: 577 bits (1487), Expect = 6.03e-208
Identity = 288/288 (100.00%), Postives = 288/288 (100.00%), Query Frame = 0

Query: 1   MTTVISAVATAGPYRYDYGRLFQRPAAGAPSPGFPWRCFAKQRWRRRRLMVVKMKKKKSE 60
           MTTVISAVATAGPYRYDYGRLFQRPAAGAPSPGFPWRCFAKQRWRRRRLMVVKMKKKKSE
Sbjct: 1   MTTVISAVATAGPYRYDYGRLFQRPAAGAPSPGFPWRCFAKQRWRRRRLMVVKMKKKKSE 60

Query: 61  EDEMVKELKMSVEEKMGKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIMEGGEFP 120
           EDEMVKELKMSVEEKMGKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIMEGGEFP
Sbjct: 61  EDEMVKELKMSVEEKMGKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIMEGGEFP 120

Query: 121 LLEMFGTFVLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINA 180
           LLEMFGTFVLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINA
Sbjct: 121 LLEMFGTFVLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINA 180

Query: 181 IPAIALLSFGLFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRR 240
           IPAIALLSFGLFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRR
Sbjct: 181 IPAIALLSFGLFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRR 240

Query: 241 VAAAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIRRRKVYRN 288
           VAAAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIRRRKVYRN
Sbjct: 241 VAAAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIRRRKVYRN 288

BLAST of CsGy5G002410 vs. ExPASy TrEMBL
Match: A0A1S3AUA9 (beta-carotene hydroxylase 2, chloroplastic-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103482780 PE=3 SV=1)

HSP 1 Score: 543 bits (1398), Expect = 1.99e-194
Identity = 275/288 (95.49%), Postives = 279/288 (96.88%), Query Frame = 0

Query: 1   MTTVISAVATAGPYRYDYGRLFQRPAAGAPSPGFPWRCFAKQRWRRRRLMVVKMKKKKSE 60
           M TVISAVATAG YRYDYGRLFQRPA GAPS GFPWRCFAKQRWRR  LMV+KMKKKKSE
Sbjct: 1   MATVISAVATAGQYRYDYGRLFQRPA-GAPSRGFPWRCFAKQRWRR--LMVMKMKKKKSE 60

Query: 61  EDEMVKELKMSVEEKMGKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIMEGGEFP 120
           EDEMVK+LKMSVEEKM KKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIMEGGEFP
Sbjct: 61  EDEMVKKLKMSVEEKMRKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIMEGGEFP 120

Query: 121 LLEMFGTFVLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINA 180
           +LEMFGTFVLSIGS+VGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINA
Sbjct: 121 ILEMFGTFVLSIGSSVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINA 180

Query: 181 IPAIALLSFGLFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRR 240
           IPAIALLSFGLFN GFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRR
Sbjct: 181 IPAIALLSFGLFNHGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRR 240

Query: 241 VAAAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIRRRKVYRN 288
           VAAAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIRRR VYRN
Sbjct: 241 VAAAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIRRRNVYRN 285

BLAST of CsGy5G002410 vs. ExPASy TrEMBL
Match: A0A1S4DS92 (beta-carotene hydroxylase 2, chloroplastic-like isoform X3 OS=Cucumis melo OX=3656 GN=LOC103482780 PE=3 SV=1)

HSP 1 Score: 493 bits (1270), Expect = 3.08e-175
Identity = 257/288 (89.24%), Postives = 259/288 (89.93%), Query Frame = 0

Query: 1   MTTVISAVATAGPYRYDYGRLFQRPAAGAPSPGFPWRCFAKQRWRRRRLMVVKMKKKKSE 60
           M TVISAVATAG YRYDYGRLFQRPA GAPS GFPWRCFAKQRWRR  LMV+KMKKKKSE
Sbjct: 1   MATVISAVATAGQYRYDYGRLFQRPA-GAPSRGFPWRCFAKQRWRR--LMVMKMKKKKSE 60

Query: 61  EDEMVKELKMSVEEKMGKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIMEGGEFP 120
           EDEMVK+LKMSVEEKM KKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIME     
Sbjct: 61  EDEMVKKLKMSVEEKMRKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIME----- 120

Query: 121 LLEMFGTFVLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINA 180
                          VGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINA
Sbjct: 121 ---------------VGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINA 180

Query: 181 IPAIALLSFGLFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRR 240
           IPAIALLSFGLFN GFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRR
Sbjct: 181 IPAIALLSFGLFNHGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRR 240

Query: 241 VAAAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIRRRKVYRN 288
           VAAAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIRRR VYRN
Sbjct: 241 VAAAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIRRRNVYRN 265

BLAST of CsGy5G002410 vs. ExPASy TrEMBL
Match: A0A1S4DT22 (beta-carotene hydroxylase 2, chloroplastic-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103482780 PE=3 SV=1)

HSP 1 Score: 488 bits (1256), Expect = 4.34e-173
Identity = 256/288 (88.89%), Postives = 260/288 (90.28%), Query Frame = 0

Query: 1   MTTVISAVATAGPYRYDYGRLFQRPAAGAPSPGFPWRCFAKQRWRRRRLMVVKMKKKKSE 60
           M TVISAVATAG YRYDYGRLFQRPA GAPS GFPWRCFAKQRWRR  LMV+KMKKKKSE
Sbjct: 1   MATVISAVATAGQYRYDYGRLFQRPA-GAPSRGFPWRCFAKQRWRR--LMVMKMKKKKSE 60

Query: 61  EDEMVKELKMSVEEKMGKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIMEGGEFP 120
           EDEMVK+LKMSVEEKM KKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIMEGGEFP
Sbjct: 61  EDEMVKKLKMSVEEKMRKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIMEGGEFP 120

Query: 121 LLEMFGTFVLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINA 180
           +LEMFGTFVLSIGS+VGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINA
Sbjct: 121 ILEMFGTFVLSIGSSVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINA 180

Query: 181 IPAIALLSFGLFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRR 240
           IPAIALLSFGLFN GFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRR
Sbjct: 181 IPAIALLSFGLFNHGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRR 240

Query: 241 VAAAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIRRRKVYRN 288
           VAAAHH                   ELEEVDGEEELQKEIRRR VYRN
Sbjct: 241 VAAAHH-------------------ELEEVDGEEELQKEIRRRNVYRN 266

BLAST of CsGy5G002410 vs. ExPASy TrEMBL
Match: A0A6J1I1U3 (beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111469080 PE=3 SV=1)

HSP 1 Score: 437 bits (1124), Expect = 9.88e-153
Identity = 231/290 (79.66%), Postives = 253/290 (87.24%), Query Frame = 0

Query: 1   MTTVISAVATAGPYRYDYGRLFQRPA-AGAPSPGFPWRCFAKQRWRRRRLMVVKMKKKKS 60
           M T ISAVATA PYR    R+ QRPA A AP+   PWRCFAKQ  RR  L  VK KK   
Sbjct: 1   MATGISAVATAAPYR----RIIQRPAKAPAPANRVPWRCFAKQSTRR--LSTVK-KKCGV 60

Query: 61  EEDEMVKELKMSVEEKMGKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIMEGGEF 120
            E EMV++LK+ VEEKMG+KKAER+ YL+AAIVSSFGITSMAAIAVYYRFS Q+ EGG+F
Sbjct: 61  SEVEMVRDLKVIVEEKMGRKKAERDAYLVAAIVSSFGITSMAAIAVYYRFSWQL-EGGDF 120

Query: 121 PLLEMFGTFVLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIIN 180
           P+LEMFGTF LS+G+AVGMEFWARWAHK+LWH +LW+MH+SHH+ R GAFE NDVFAIIN
Sbjct: 121 PVLEMFGTFALSVGAAVGMEFWARWAHKQLWHDALWNMHKSHHRARDGAFELNDVFAIIN 180

Query: 181 AIPAIALLSFGLFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLR 240
           A+PAIALLSFG FNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLR
Sbjct: 181 AVPAIALLSFGFFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLR 240

Query: 241 RVAAAHHIHHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIRRR-KVYRN 288
           RVAAAHHIHHTDKFDGVPYGLFLGPKELE+VDGEEELQ EI++R KVYRN
Sbjct: 241 RVAAAHHIHHTDKFDGVPYGLFLGPKELEDVDGEEELQNEIKKRSKVYRN 282

BLAST of CsGy5G002410 vs. TAIR 10
Match: AT4G25700.1 (beta-hydroxylase 1 )

HSP 1 Score: 347.1 bits (889), Expect = 1.4e-95
Identity = 168/216 (77.78%), Postives = 189/216 (87.50%), Query Frame = 0

Query: 68  LKMSVEEKMGKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIMEGGEFPLLEMFGT 127
           L + + EK+ +KK+ER  YLIAA++SSFGITSMA +AVYYRFS Q MEGGE  +LEMFGT
Sbjct: 80  LALRLAEKLERKKSERSTYLIAAMLSSFGITSMAVMAVYYRFSWQ-MEGGEISMLEMFGT 139

Query: 128 FVLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINAIPAIALL 187
           F LS+G+AVGMEFWARWAH+ LWHASLW+MHESHHKPR G FE NDVFAI+NA PAI LL
Sbjct: 140 FALSVGAAVGMEFWARWAHRALWHASLWNMHESHHKPREGPFELNDVFAIVNAGPAIGLL 199

Query: 188 SFGLFNQGFFPGLCFGAGLGITVFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHI 247
           S+G FN+G  PGLCFGAGLGITVFG+AYMFVHDGLVH+RFPVGPIA VPYLR+VAAAH +
Sbjct: 200 SYGFFNKGLVPGLCFGAGLGITVFGIAYMFVHDGLVHKRFPVGPIADVPYLRKVAAAHQL 259

Query: 248 HHTDKFDGVPYGLFLGPKELEEVDGEEELQKEIRRR 284
           HHTDKF+GVPYGLFLGPKELEEV G EEL KEI RR
Sbjct: 260 HHTDKFNGVPYGLFLGPKELEEVGGNEELDKEISRR 294

BLAST of CsGy5G002410 vs. TAIR 10
Match: AT5G52570.1 (beta-carotene hydroxylase 2 )

HSP 1 Score: 334.3 bits (856), Expect = 9.5e-92
Identity = 176/258 (68.22%), Postives = 209/258 (81.01%), Query Frame = 0

Query: 44  WRRRRLM----VVKMKKKKSEEDEMVK--------ELKMS--VEEKMGKKKAEREGYLIA 103
           +RRR+++    VV+ +K+ S  D+  K        E+ M+  + +K  KKK+ER  YLIA
Sbjct: 40  FRRRKILTVCFVVEERKQSSPMDDDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIA 99

Query: 104 AIVSSFGITSMAAIAVYYRFSSQIMEGGEFPLLEMFGTFVLSIGSAVGMEFWARWAHKEL 163
           A++SSFGITSMA +AVYYRFS Q M+GGE  +LEMFGTF LS+G+AVGMEFWARWAH+ L
Sbjct: 100 AVMSSFGITSMAIMAVYYRFSWQ-MKGGEVSVLEMFGTFALSVGAAVGMEFWARWAHRAL 159

Query: 164 WHASLWDMHESHHKPRVGAFEKNDVFAIINAIPAIALLSFGLFNQGFFPGLCFGAGLGIT 223
           WH SLW+MHESHHKPR GAFE NDVFAI NA+PAI LL +G  N+G  PGLCFGAGLGIT
Sbjct: 160 WHDSLWNMHESHHKPREGAFELNDVFAITNAVPAIGLLYYGFLNKGLVPGLCFGAGLGIT 219

Query: 224 VFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHIHHTDKFDGVPYGLFLGPKELEE 283
           +FGMAYMFVHDGLVH+RFPVGPIA VPYLR+VAAAH +HHTDKF GVPYGLFLGPKE+EE
Sbjct: 220 MFGMAYMFVHDGLVHKRFPVGPIANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPKEVEE 279

Query: 284 VDGEEELQKEIRRR-KVY 287
           V G+EEL+KEI RR K+Y
Sbjct: 280 VGGKEELEKEISRRIKLY 296

BLAST of CsGy5G002410 vs. TAIR 10
Match: AT4G25700.2 (beta-hydroxylase 1 )

HSP 1 Score: 205.7 bits (522), Expect = 5.1e-53
Identity = 100/137 (72.99%), Postives = 116/137 (84.67%), Query Frame = 0

Query: 68  LKMSVEEKMGKKKAEREGYLIAAIVSSFGITSMAAIAVYYRFSSQIMEGGEFPLLEMFGT 127
           L + + EK+ +KK+ER  YLIAA++SSFGITSMA +AVYYRFS Q MEGGE  +LEMFGT
Sbjct: 80  LALRLAEKLERKKSERSTYLIAAMLSSFGITSMAVMAVYYRFSWQ-MEGGEISMLEMFGT 139

Query: 128 FVLSIGSAVGMEFWARWAHKELWHASLWDMHESHHKPRVGAFEKNDVFAIINAIPAIALL 187
           F LS+G+AVGMEFWARWAH+ LWHASLW+MHESHHKPR G FE NDVFAI+NA PAI LL
Sbjct: 140 FALSVGAAVGMEFWARWAHRALWHASLWNMHESHHKPREGPFELNDVFAIVNAGPAIGLL 199

Query: 188 SFGLFNQGFFPGLCFGA 205
           S+G FN+G  PGLCFGA
Sbjct: 200 SYGFFNKGLVPGLCFGA 215

BLAST of CsGy5G002410 vs. TAIR 10
Match: AT5G52570.2 (beta-carotene hydroxylase 2 )

HSP 1 Score: 186.0 bits (471), Expect = 4.2e-47
Identity = 123/258 (47.67%), Postives = 151/258 (58.53%), Query Frame = 0

Query: 44  WRRRRLM----VVKMKKKKSEEDEMVK--------ELKMS--VEEKMGKKKAEREGYLIA 103
           +RRR+++    VV+ +K+ S  D+  K        E+ M+  + +K  KKK+ER  YLIA
Sbjct: 40  FRRRKILTVCFVVEERKQSSPMDDDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIA 99

Query: 104 AIVSSFGITSMAAIAVYYRFSSQIMEGGEFPLLEMFGTFVLSIGSAVGMEFWARWAHKEL 163
           A++SSFGITSMA +AVYYRFS Q M+GGE  +LEMFGTF LS+G+               
Sbjct: 100 AVMSSFGITSMAIMAVYYRFSWQ-MKGGEVSVLEMFGTFALSVGA--------------- 159

Query: 164 WHASLWDMHESHHKPRVGAFEKNDVFAIINAIPAIALLSFGLFNQGFFPGLCFGAGLGIT 223
                                                                 AGLGIT
Sbjct: 160 ------------------------------------------------------AGLGIT 219

Query: 224 VFGMAYMFVHDGLVHRRFPVGPIAAVPYLRRVAAAHHIHHTDKFDGVPYGLFLGPKELEE 283
           +FGMAYMFVHDGLVH+RFPVGPIA VPYLR+VAAAH +HHTDKF GVPYGLFLGPKE+EE
Sbjct: 220 MFGMAYMFVHDGLVHKRFPVGPIANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPKEVEE 227

Query: 284 VDGEEELQKEIRRR-KVY 287
           V G+EEL+KEI RR K+Y
Sbjct: 280 VGGKEELEKEISRRIKLY 227

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SZZ82.0e-9477.78Beta-carotene 3-hydroxylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=... [more]
O498141.9e-9272.27Beta-carotene hydroxylase 2, chloroplastic OS=Capsicum annuum OX=4072 GN=CA2 PE=... [more]
B3SGL01.9e-9275.11Beta-carotene 3-hydroxylase, chloroplastic OS=Gentiana lutea OX=38851 GN=BHY PE=... [more]
Q9LTG01.3e-9068.22Beta-carotene 3-hydroxylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=... [more]
O498158.6e-9073.39Beta-carotene hydroxylase 1, chloroplastic OS=Capsicum annuum OX=4072 GN=CA1 PE=... [more]
Match NameE-valueIdentityDescription
XP_004143973.11.25e-207100.00beta-carotene 3-hydroxylase 1, chloroplastic [Cucumis sativus] >KGN50067.1 hypot... [more]
XP_008437325.14.12e-19495.49PREDICTED: beta-carotene hydroxylase 2, chloroplastic-like isoform X1 [Cucumis m... [more]
XP_016898856.16.36e-17589.24PREDICTED: beta-carotene hydroxylase 2, chloroplastic-like isoform X3 [Cucumis m... [more]
XP_016898855.18.97e-17388.89PREDICTED: beta-carotene hydroxylase 2, chloroplastic-like isoform X2 [Cucumis m... [more]
XP_038907152.11.21e-16181.69beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A0A0KNU96.03e-208100.00Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=C... [more]
A0A1S3AUA91.99e-19495.49beta-carotene hydroxylase 2, chloroplastic-like isoform X1 OS=Cucumis melo OX=36... [more]
A0A1S4DS923.08e-17589.24beta-carotene hydroxylase 2, chloroplastic-like isoform X3 OS=Cucumis melo OX=36... [more]
A0A1S4DT224.34e-17388.89beta-carotene hydroxylase 2, chloroplastic-like isoform X2 OS=Cucumis melo OX=36... [more]
A0A6J1I1U39.88e-15379.66beta-carotene 3-hydroxylase 1, chloroplastic-like isoform X1 OS=Cucurbita maxima... [more]
Match NameE-valueIdentityDescription
AT4G25700.11.4e-9577.78beta-hydroxylase 1 [more]
AT5G52570.19.5e-9268.22beta-carotene hydroxylase 2 [more]
AT4G25700.25.1e-5372.99beta-hydroxylase 1 [more]
AT5G52570.24.2e-4747.67beta-carotene hydroxylase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006694Fatty acid hydroxylasePFAMPF04116FA_hydroxylasecoord: 135..261
e-value: 1.1E-12
score: 48.4
IPR045019Beta-carotene hydroxylase-likePANTHERPTHR31899BETA-CAROTENE 3-HYDROXYLASE 1, CHLOROPLASTICcoord: 51..284
NoneNo IPR availablePANTHERPTHR31899:SF14HYDROXYLASE, PUTATIVE, EXPRESSED-RELATEDcoord: 51..284

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy5G002410.2CsGy5G002410.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008610 lipid biosynthetic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016491 oxidoreductase activity