CsGy5G001520 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy5G001520
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Descriptionmethionine gamma-lyase-like
LocationGy14Chr5: 956861 .. 959225 (-)
RNA-Seq ExpressionCsGy5G001520
SyntenyCsGy5G001520
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATAACTGAAGTAAGAGCTCACGACACTTCTCTCCCATGCAAGAACAACAATAATTTCTCTTACGATACCGAACCGACCTCCATCACGAGGCTATCATCATGGCCAATGGGTGAGCTAGATCCAGCGGCCGCTCTGGCAATGGCCAGCCCCGAGTTCGGAGAGCATGGTGGCGTGAACATGTCAATTGAAAACTCCATAACATTCACGGTGATGGAGGCGGAGACGATGCGTAGGATGTTTGAAGGGGAGTTGGGGCCTGAGCGGGACTTCTTCATCTACAGCCGCCACTTCAACCCGACCGTCATGGCCCTTAGCCGGCAGATGGCGGCATTGGAAGGGACCGAGGCAGCCTATTGTACGTCGAGTGGGATGGCCGCTGTTTCATCTGTTTTGCTGCAGCTTGTGAGCTCCGGTGGGCATATCGTGGCGTCGAGGACGCTCTACGGCGGTACTCATGCATTGCTGTCGCATTTTCTGCCGAGGGTAAGCAAGATAACAACGACTTTTGTGGACGTGAGGAACCATGAAATGGTTAGAGATGCCATGGTAGAGGGAAAGACGAAGGTACATAATCTAACACCTACTAAAAGTTTTAGGTTTATATATGTTTCCTTGATTGAATTATGAATTTGGTTCTTACACTGTCATTTTCCTGGGGAGAAATTTTACCCTATCAATTTGATTTTTATAATGATTCAAATGTATTTAATTTAATTTTAGTTTTGGTTTGATTTATTTTCATATCGATTAACTTTCTTTTAACGCGTTAATTCGAATCATGGATGACAATTAACACAAAAGGATTGATAATTAAACATTAATAAGAAAAAAACTTAATTATTTTCAAACGTTTGAATCCGAGTTCTTCCTTTATATAACAAGTTAAATCAATTTTTCAAAATTAATTTAGGTTTATAGGTTACAAAGAAATTAAATATTTTACCCTTTTGTTGAACCTATAGGTATTGTATTTTGAGACGGTGGCAAACCCGACGCTTACGGTGGCAAACATCCCCGAGCTCAGTCGGATGGCACACCAGAAGGGGGTGACTGTGGTAGTCGATAATACGTTCTCACCCATAATCATCTCACCGACAAGGCTCGGTGCTGACGTCGTCGTCCATAGCTGCTCAAAGTTTATCAGCGGTGGTGCAGACATTATTGCAGGTATATGATTAGTCCATTTTTTTCTGATTTAGTTAAATATTTTATTAGGATTTATGAGTTGCAAATTTAGAAATAATAATTTTTTAATATGTTTTAAATGTTTCTATTTAAAAAAACACTAATTATACATAACCCATTATTTAATAAAAGCCTAACATTTAAACAATTTTTAAAGTATAGTCTATGTTTTATATATTATATATTCACTCTTGTCTATTTAATTATAAGTAAGCTAATGAAAACATGATTTTAATTTGATCTTTCGTGGAAGCTTAAAAAAATTGGCTAATATATTGTTTAATGAATAATGGTTATGTTCTATTTTGACATGTCATTGAATGAGGTTTTGGTGACTCGTCATTACATAGTGTCATGTTTTAAAAATAGATAATGTAATTAAAAAAAGTATATAAACAATAATGAACTTCAAATTTCCCCCCCAAAAATCTCTACTTGAAAAAATAATTGAAAAAGAGAATATTTTTTATGGTGGATGGAAAAGGCGTTGACCAATTGAGTTAAGTTATGTTACTATAAATGAATGTTAATCTATGAACAAACAAACTCCATTTAATTGATTTTTAATGTATACAAAAAAATGATAAAATGATTTATCCTTTTTTAACTTTTCATTTTAGGCGCTGTTTGTGGGTCAAAAGAGTTGGTGAATTCTATGATGGACCTTCACCAAGGCACATTGATGCTTCTGGGGCCAACAATGAATGCTAAAGTTGCATCTGAACTCTCTCAAAGAATCCCCCATTTAAGCCTTCGAATGAAGGAGCATTCTAACAGAGCTTTAGTTTTCGCTCAAAGGATGAAGAATCTCGGCCTCAAAGTCATATACCCTGGCCTCAACGATCACCCTGATCACCAACTTCTAGGCTCCATAGCCAACAAAGACTACGGCTACGGCGGAGTTTTGTGCATTGACATGGGGACCGAGGAGAGAGCCAACAGACTCATGAATCTCCTTCAAAACCATACACACTTCGGTCTCATGGCCGTTAGCTTGGGCTTCTTCGAGACCCTCATGTCATGTTCAGGTAGTAGCACGAGTAGCGAGTTAAATGGGGAGGAGAAGGAATTGGCTGGCATTTCTCCTGGATTGATTAGGATGTCTGTTGGATATGTTGGAACTCTGGAGCAGAAGTGGAACCAATTGGAGAAGGCACTGTTGAAGATGAAGATTTGA

mRNA sequence

ATGATAACTGAAGTAAGAGCTCACGACACTTCTCTCCCATGCAAGAACAACAATAATTTCTCTTACGATACCGAACCGACCTCCATCACGAGGCTATCATCATGGCCAATGGGTGAGCTAGATCCAGCGGCCGCTCTGGCAATGGCCAGCCCCGAGTTCGGAGAGCATGGTGGCGTGAACATGTCAATTGAAAACTCCATAACATTCACGGTGATGGAGGCGGAGACGATGCGTAGGATGTTTGAAGGGGAGTTGGGGCCTGAGCGGGACTTCTTCATCTACAGCCGCCACTTCAACCCGACCGTCATGGCCCTTAGCCGGCAGATGGCGGCATTGGAAGGGACCGAGGCAGCCTATTGTACGTCGAGTGGGATGGCCGCTGTTTCATCTGTTTTGCTGCAGCTTGTGAGCTCCGGTGGGCATATCGTGGCGTCGAGGACGCTCTACGGCGGTACTCATGCATTGCTGTCGCATTTTCTGCCGAGGGTAAGCAAGATAACAACGACTTTTGTGGACGTGAGGAACCATGAAATGGTTAGAGATGCCATGGTAGAGGGAAAGACGAAGGTATTGTATTTTGAGACGGTGGCAAACCCGACGCTTACGGTGGCAAACATCCCCGAGCTCAGTCGGATGGCACACCAGAAGGGGGTGACTGTGGTAGTCGATAATACGTTCTCACCCATAATCATCTCACCGACAAGGCTCGGTGCTGACGTCGTCGTCCATAGCTGCTCAAAGTTTATCAGCGGTGGTGCAGACATTATTGCAGGCGCTGTTTGTGGGTCAAAAGAGTTGGTGAATTCTATGATGGACCTTCACCAAGGCACATTGATGCTTCTGGGGCCAACAATGAATGCTAAAGTTGCATCTGAACTCTCTCAAAGAATCCCCCATTTAAGCCTTCGAATGAAGGAGCATTCTAACAGAGCTTTAGTTTTCGCTCAAAGGATGAAGAATCTCGGCCTCAAAGTCATATACCCTGGCCTCAACGATCACCCTGATCACCAACTTCTAGGCTCCATAGCCAACAAAGACTACGGCTACGGCGGAGTTTTGTGCATTGACATGGGGACCGAGGAGAGAGCCAACAGACTCATGAATCTCCTTCAAAACCATACACACTTCGGTCTCATGGCCGTTAGCTTGGGCTTCTTCGAGACCCTCATGTCATGTTCAGGTAGTAGCACGAGTAGCGAGTTAAATGGGGAGGAGAAGGAATTGGCTGGCATTTCTCCTGGATTGATTAGGATGTCTGTTGGATATGTTGGAACTCTGGAGCAGAAGTGGAACCAATTGGAGAAGGCACTGTTGAAGATGAAGATTTGA

Coding sequence (CDS)

ATGATAACTGAAGTAAGAGCTCACGACACTTCTCTCCCATGCAAGAACAACAATAATTTCTCTTACGATACCGAACCGACCTCCATCACGAGGCTATCATCATGGCCAATGGGTGAGCTAGATCCAGCGGCCGCTCTGGCAATGGCCAGCCCCGAGTTCGGAGAGCATGGTGGCGTGAACATGTCAATTGAAAACTCCATAACATTCACGGTGATGGAGGCGGAGACGATGCGTAGGATGTTTGAAGGGGAGTTGGGGCCTGAGCGGGACTTCTTCATCTACAGCCGCCACTTCAACCCGACCGTCATGGCCCTTAGCCGGCAGATGGCGGCATTGGAAGGGACCGAGGCAGCCTATTGTACGTCGAGTGGGATGGCCGCTGTTTCATCTGTTTTGCTGCAGCTTGTGAGCTCCGGTGGGCATATCGTGGCGTCGAGGACGCTCTACGGCGGTACTCATGCATTGCTGTCGCATTTTCTGCCGAGGGTAAGCAAGATAACAACGACTTTTGTGGACGTGAGGAACCATGAAATGGTTAGAGATGCCATGGTAGAGGGAAAGACGAAGGTATTGTATTTTGAGACGGTGGCAAACCCGACGCTTACGGTGGCAAACATCCCCGAGCTCAGTCGGATGGCACACCAGAAGGGGGTGACTGTGGTAGTCGATAATACGTTCTCACCCATAATCATCTCACCGACAAGGCTCGGTGCTGACGTCGTCGTCCATAGCTGCTCAAAGTTTATCAGCGGTGGTGCAGACATTATTGCAGGCGCTGTTTGTGGGTCAAAAGAGTTGGTGAATTCTATGATGGACCTTCACCAAGGCACATTGATGCTTCTGGGGCCAACAATGAATGCTAAAGTTGCATCTGAACTCTCTCAAAGAATCCCCCATTTAAGCCTTCGAATGAAGGAGCATTCTAACAGAGCTTTAGTTTTCGCTCAAAGGATGAAGAATCTCGGCCTCAAAGTCATATACCCTGGCCTCAACGATCACCCTGATCACCAACTTCTAGGCTCCATAGCCAACAAAGACTACGGCTACGGCGGAGTTTTGTGCATTGACATGGGGACCGAGGAGAGAGCCAACAGACTCATGAATCTCCTTCAAAACCATACACACTTCGGTCTCATGGCCGTTAGCTTGGGCTTCTTCGAGACCCTCATGTCATGTTCAGGTAGTAGCACGAGTAGCGAGTTAAATGGGGAGGAGAAGGAATTGGCTGGCATTTCTCCTGGATTGATTAGGATGTCTGTTGGATATGTTGGAACTCTGGAGCAGAAGTGGAACCAATTGGAGAAGGCACTGTTGAAGATGAAGATTTGA

Protein sequence

MITEVRAHDTSLPCKNNNNFSYDTEPTSITRLSSWPMGELDPAAALAMASPEFGEHGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVSKITTTFVDVRNHEMVRDAMVEGKTKVLYFETVANPTLTVANIPELSRMAHQKGVTVVVDNTFSPIIISPTRLGADVVVHSCSKFISGGADIIAGAVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPHLSLRMKEHSNRALVFAQRMKNLGLKVIYPGLNDHPDHQLLGSIANKDYGYGGVLCIDMGTEERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMSVGYVGTLEQKWNQLEKALLKMKI*
Homology
BLAST of CsGy5G001520 vs. ExPASy Swiss-Prot
Match: Q9SGU9 (Methionine gamma-lyase OS=Arabidopsis thaliana OX=3702 GN=MGL PE=1 SV=1)

HSP 1 Score: 615.1 bits (1585), Expect = 6.0e-175
Identity = 305/426 (71.60%), Postives = 361/426 (84.74%), Query Frame = 0

Query: 15  KNNNNFSYDTEPTSITRLSSWPMGELDPAAALAMASPEFGEHGGVNMSIENSITFTVMEA 74
           K  N+   +     + + S+  + + DPAAA+A    EFGEHGGVNMSIE S TFTVME 
Sbjct: 16  KKRNDRDDEDGDALVAKKSALAVCDADPAAAIANIRHEFGEHGGVNMSIEASATFTVMEP 75

Query: 75  ETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQ 134
           +TMRRMF GELGP+ DF++YSRHFNPTV+ LSRQMAALEGT+AAYCTSSGM+A+SSV+LQ
Sbjct: 76  DTMRRMFTGELGPDNDFYVYSRHFNPTVLNLSRQMAALEGTQAAYCTSSGMSAISSVMLQ 135

Query: 135 LVSSGGHIVASRTLYGGTHALLSHFLPRVSKITTTFVDVRNHEMVRDAMVEGKTKVLYFE 194
           L SSGGH+VA+ TLYGGTHALLSHFLPR   ITT+FVD+ +H  V +A+VEG+T+VLYFE
Sbjct: 136 LCSSGGHVVAASTLYGGTHALLSHFLPRTCNITTSFVDITDHGAVANAIVEGRTQVLYFE 195

Query: 195 TVANPTLTVANIPELSRMAHQKGVTVVVDNTFSPIIISPTRLGADVVVHSCSKFISGGAD 254
           +VANPTLTVA+IPELSRMAH+KGVTVVVDNTF+P+++SP +LGADVVVHS SKFISGGAD
Sbjct: 196 SVANPTLTVADIPELSRMAHEKGVTVVVDNTFAPMVLSPAKLGADVVVHSISKFISGGAD 255

Query: 255 IIAGAVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPHLSLRMKEHSNRALVF 314
           IIAGAVCGS+ LV  MMDL  G+LMLLGPTMNAKVA ELS+RIPHL LRM+EHS+RA V+
Sbjct: 256 IIAGAVCGSENLVKEMMDLRGGSLMLLGPTMNAKVAFELSERIPHLGLRMREHSHRAQVY 315

Query: 315 AQRMKNLGLKVIYPGLNDHPDHQLLGSIANKDYGYGGVLCIDMGTEERANRLMNLLQNHT 374
           A+RM++LG+KVIYPGL  HP H+L   + N+DYGYGG+L IDM TEE+AN+LM  LQN T
Sbjct: 316 AERMRDLGMKVIYPGLETHPQHKLFKGMVNRDYGYGGLLSIDMETEEKANKLMAYLQNAT 375

Query: 375 HFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMSVGYVGTLEQKWNQLE 434
            FG MAVSLG++ETLMSCSGSSTSSEL+  +KE AGISPGL+RMSVGYVGTLEQKW Q E
Sbjct: 376 QFGFMAVSLGYYETLMSCSGSSTSSELDPSQKEAAGISPGLVRMSVGYVGTLEQKWTQFE 435

Query: 435 KALLKM 441
           KA L+M
Sbjct: 436 KAFLRM 441

BLAST of CsGy5G001520 vs. ExPASy Swiss-Prot
Match: Q8L0X4 (L-methionine gamma-lyase OS=Fusobacterium nucleatum subsp. polymorphum OX=76857 GN=mgl PE=1 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 7.1e-51
Identity = 148/392 (37.76%), Postives = 218/392 (55.61%), Query Frame = 0

Query: 56  HGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGT 115
           +G + M I  + TF    AE   R F      E   +IY+R  NPT   L  ++AALE  
Sbjct: 22  YGTLAMPIYQTSTFIFDSAEQGGRRF----ALEEAGYIYTRLGNPTTTVLENKIAALEEG 81

Query: 116 EAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVSKITTTFVDVRN 175
           EAA  TSSGM A+SS L  ++ +G H+V  +TLYG T AL+ H L R   I  TFVD  N
Sbjct: 82  EAAVATSSGMGAISSTLWTVLKAGDHVVTDKTLYGCTFALMCHGLTRFG-IEVTFVDTSN 141

Query: 176 HEMVRDAMVEGKTKVLYFETVANPTLTVANIPELSRMAHQKGVT-VVVDNTF-SPIIISP 235
            + V++AM +  T+V+Y ET ANP L + ++  LS++AH    T V+VDNTF +P +  P
Sbjct: 142 LDEVKNAM-KKNTRVVYLETPANPNLKIVDLEALSKLAHTNPNTLVIVDNTFATPYMQKP 201

Query: 236 TRLGADVVVHSCSKFISGGADIIAGAVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASEL 295
            +LGAD+VVHS +K+I+G  D+IAG V  +KEL + +  +  G   + G  +  + A  +
Sbjct: 202 LKLGADIVVHSVTKYINGHGDVIAGLVITNKELADQIRFI--GLKDMTGAVLGPQDAYYI 261

Query: 296 SQRIPHLSLRMKEH---SNRALVFAQRMKNLGLKVIYPGLNDHPDHQLLGSIANKDY-GY 355
            + +    +RM+ H   + + + F  +   +  +V YPGL  HP H+    IA K    +
Sbjct: 262 IRGMKTFEIRMERHCKNAKKVVEFLNKHPKIE-RVYYPGLETHPGHE----IAKKQMKDF 321

Query: 356 GGVLCIDM-GTEERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKEL 415
           G ++  ++ G  E    L+N L+       +AVSLG  ETL+    S T S    EE+E 
Sbjct: 322 GAMISFELKGGFEAGKTLLNNLK----LCSLAVSLGDTETLIQHPASMTHSPYTKEEREA 381

Query: 416 AGISPGLIRMSVGYVGTLEQKWNQLEKALLKM 441
           AGI+ GL+R+SVG +  +E     LE+ L K+
Sbjct: 382 AGITDGLVRLSVG-LENVEDIIADLEQGLEKI 395

BLAST of CsGy5G001520 vs. ExPASy Swiss-Prot
Match: Q8RDT4 (L-methionine gamma-lyase OS=Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / DSM 15643 / BCRC 10681 / CIP 101130 / JCM 8532 / KCTC 2640 / LMG 13131 / VPI 4355) OX=190304 GN=FN1419 PE=1 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 8.7e-49
Identity = 143/391 (36.57%), Postives = 212/391 (54.22%), Query Frame = 0

Query: 56  HGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGT 115
           +G + M I  + TF    AE   R F      E   +IY+R  NPT   L  ++AALE  
Sbjct: 22  YGTLAMPIYQTSTFIFDSAEQGGRRF----ALEEAGYIYTRLGNPTTTVLENKIAALEEG 81

Query: 116 EAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVSKITTTFVDVRN 175
           EA    SSGM A+SS L  ++ +G H+V  +TLYG T AL++H L R   +  TFVD  N
Sbjct: 82  EAGIAMSSGMGAISSTLWTVLKAGDHVVTDKTLYGCTFALMNHGLTRFG-VEVTFVDTSN 141

Query: 176 HEMVRDAMVEGKTKVLYFETVANPTLTVANIPELSRMAHQKGVT-VVVDNTF-SPIIISP 235
            E V++AM +  T+V+Y ET ANP L + ++  LS++AH    T V+VDNTF +P +  P
Sbjct: 142 LEEVKNAM-KKNTRVVYLETPANPNLKIVDLEALSKIAHTNPNTLVIVDNTFATPYMQKP 201

Query: 236 TRLGADVVVHSCSKFISGGADIIAGAVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASEL 295
            +LG D+VVHS +K+++G  D+IAG V   +EL + +  +  G   + G  +  + A  +
Sbjct: 202 LKLGVDIVVHSATKYLNGHGDVIAGLVVTRQELADQIRFV--GLKDMTGAVLGPQEAYYI 261

Query: 296 SQRIPHLSLRMKEHSNRALVFAQRMKNLGL--KVIYPGLNDHPDHQLLGSIANKDY-GYG 355
            + +    +RM+ H   A      +       KV YPGL  HP ++    IA K    +G
Sbjct: 262 IRGLKTFEIRMERHCKNARTIVDFLNKHPKVEKVYYPGLETHPGYE----IAKKQMKDFG 321

Query: 356 GVLCIDM-GTEERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELA 415
            ++  ++ G  E    L+N L+       +AVSLG  ETL+    S T S    EE+E+A
Sbjct: 322 AMISFELKGGFEAGKTLLNNLK----LCSLAVSLGDTETLIQHPASMTHSPYTKEEREVA 381

Query: 416 GISPGLIRMSVGYVGTLEQKWNQLEKALLKM 441
           GI+ GL+R+SVG +  +E     LE+ L K+
Sbjct: 382 GITDGLVRLSVG-LENVEDIIADLEQGLEKI 395

BLAST of CsGy5G001520 vs. ExPASy Swiss-Prot
Match: Q7MX71 (L-methionine gamma-lyase OS=Porphyromonas gingivalis (strain ATCC BAA-308 / W83) OX=242619 GN=mgl PE=1 SV=2)

HSP 1 Score: 189.9 bits (481), Expect = 6.2e-47
Identity = 138/365 (37.81%), Postives = 203/365 (55.62%), Query Frame = 0

Query: 63  IENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAYCTS 122
           I  + TF    AE   R F G    E D +IY+R  NP    +  ++A LEG EAA   S
Sbjct: 32  IYQTSTFVFDTAEQGGRRFAG----EEDGYIYTRLGNPNCTQVEEKLAMLEGGEAAASAS 91

Query: 123 SGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVSKITTTFVDVRNHEMVRDA 182
           SG+ A+SS +   V +G HIVA +TLYG T A L+H L R   +  T VD R+ E V +A
Sbjct: 92  SGIGAISSAIWVCVKAGDHIVAGKTLYGCTFAFLTHGLSRYG-VEVTLVDTRHPEEV-EA 151

Query: 183 MVEGKTKVLYFETVANPTLTVANIPELSRMAHQ-KGVTVVVDNTF-SPIIISPTRLGADV 242
            +   TK++Y ET ANP + + +I  +  +AH+ +GV V+VDNT+ +P I  P  LGAD+
Sbjct: 152 AIRPNTKLVYLETPANPNMYLTDIKAVCDIAHKHEGVRVMVDNTYCTPYICRPLELGADI 211

Query: 243 VVHSCSKFISGGADIIAGAVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPHL 302
           VVHS +K+++G  D+IAG V G ++ +  +  +  G   L G  M+   A  +S+ +  L
Sbjct: 212 VVHSATKYLNGHGDVIAGFVVGKEDYIKEVKLV--GVKDLTGANMSPFDAYLISRGMKTL 271

Query: 303 SLRMKEHSNRALVFAQRM-KNLGLKVIY-PGLNDHPDHQLLGSIANKDYGY-GGVLCIDM 362
            +RM++H   A   A+ + K+  ++ +Y PGL   P ++L    A K     G ++  ++
Sbjct: 272 QIRMEQHCRNAQTVAEFLEKHPAVEAVYFPGLPSFPQYEL----AKKQMALPGAMIAFEV 331

Query: 363 -GTEERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLI 422
            G  E   +LMN L    H   +AVSLG  ETL+    S T S    EE+  + IS GL+
Sbjct: 332 KGGCEAGKKLMNNL----HLCSLAVSLGDTETLIQHPASMTHSPYTPEERAASDISEGLV 380

BLAST of CsGy5G001520 vs. ExPASy Swiss-Prot
Match: Q73KL7 (L-methionine gamma-lyase OS=Treponema denticola (strain ATCC 35405 / DSM 14222 / CIP 103919 / JCM 8153 / KCTC 15104) OX=243275 GN=megL PE=1 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.7e-44
Identity = 129/372 (34.68%), Postives = 198/372 (53.23%), Query Frame = 0

Query: 55  EHGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEG 114
           ++G +   I  + TF    AE   R F      E + +IY+R  NPT   +  ++A LE 
Sbjct: 24  KYGALATPIYQTSTFAFDSAEQGGRRF----ALEEEGYIYTRLGNPTTTVVEEKLACLEN 83

Query: 115 TEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVSKITTTFVDVR 174
            EA    SSG+ AV+S +  +V++G HIVA +TLYG T A L+H L R   +  TFVD R
Sbjct: 84  GEACMSASSGIGAVTSCIWSIVNAGDHIVAGKTLYGCTFAFLNHGLSRFG-VDVTFVDTR 143

Query: 175 NHEMVRDAMVEGKTKVLYFETVANPTLTVANIPELSRMAHQKG--VTVVVDNTF-SPIII 234
           + E V+ A+ +  TK++Y ET ANP + + +I  +S++AH       V+VDNT+ +P + 
Sbjct: 144 DPENVKKAL-KPNTKIVYLETPANPNMYLCDIAAVSKIAHAHNPECKVIVDNTYMTPYLQ 203

Query: 235 SPTRLGADVVVHSCSKFISGGADIIAGAVCGSKELVNSMMDLHQGTLMLLGPTMNAKVAS 294
            P  LGADVV+HS +K+++G  D+IAG V G KE ++ +  +  G   + G T+    A 
Sbjct: 204 RPLDLGADVVLHSATKYLNGHGDVIAGFVVGKKEFIDQVRFV--GVKDMTGSTLGPFEAY 263

Query: 295 ELSQRIPHLSLRMKEHSNRALVFAQRMKNLGL--KVIYPGLNDHPDHQLLGSIANKDYGY 354
            + + +  L +RM++H   A   A+ ++       + +PGL   P ++L    A K    
Sbjct: 264 LIGRGMKTLDIRMEKHCANAQKVAEFLEKHPAVESIAFPGLKSFPQYEL----AKKQMKL 323

Query: 355 GGVLCIDMGTEERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELA 414
            G +     T +        L N   F  +AVSLG  ETL+    S T S    EE+  +
Sbjct: 324 CGAMI--AFTVKGGLEAGKTLINSVKFATIAVSLGDAETLIQHPASMTHSPYTPEERAAS 381

Query: 415 GISPGLIRMSVG 422
            I+ GL+R+SVG
Sbjct: 384 DIAEGLVRLSVG 381

BLAST of CsGy5G001520 vs. NCBI nr
Match: XP_004143943.3 (methionine gamma-lyase [Cucumis sativus] >KGN49981.1 hypothetical protein Csa_000014 [Cucumis sativus])

HSP 1 Score: 874 bits (2258), Expect = 0.0
Identity = 442/442 (100.00%), Postives = 442/442 (100.00%), Query Frame = 0

Query: 1   MITEVRAHDTSLPCKNNNNFSYDTEPTSITRLSSWPMGELDPAAALAMASPEFGEHGGVN 60
           MITEVRAHDTSLPCKNNNNFSYDTEPTSITRLSSWPMGELDPAAALAMASPEFGEHGGVN
Sbjct: 1   MITEVRAHDTSLPCKNNNNFSYDTEPTSITRLSSWPMGELDPAAALAMASPEFGEHGGVN 60

Query: 61  MSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAYC 120
           MSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAYC
Sbjct: 61  MSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAYC 120

Query: 121 TSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVSKITTTFVDVRNHEMVR 180
           TSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVSKITTTFVDVRNHEMVR
Sbjct: 121 TSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVSKITTTFVDVRNHEMVR 180

Query: 181 DAMVEGKTKVLYFETVANPTLTVANIPELSRMAHQKGVTVVVDNTFSPIIISPTRLGADV 240
           DAMVEGKTKVLYFETVANPTLTVANIPELSRMAHQKGVTVVVDNTFSPIIISPTRLGADV
Sbjct: 181 DAMVEGKTKVLYFETVANPTLTVANIPELSRMAHQKGVTVVVDNTFSPIIISPTRLGADV 240

Query: 241 VVHSCSKFISGGADIIAGAVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPHL 300
           VVHSCSKFISGGADIIAGAVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPHL
Sbjct: 241 VVHSCSKFISGGADIIAGAVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPHL 300

Query: 301 SLRMKEHSNRALVFAQRMKNLGLKVIYPGLNDHPDHQLLGSIANKDYGYGGVLCIDMGTE 360
           SLRMKEHSNRALVFAQRMKNLGLKVIYPGLNDHPDHQLLGSIANKDYGYGGVLCIDMGTE
Sbjct: 301 SLRMKEHSNRALVFAQRMKNLGLKVIYPGLNDHPDHQLLGSIANKDYGYGGVLCIDMGTE 360

Query: 361 ERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMSV 420
           ERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMSV
Sbjct: 361 ERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMSV 420

Query: 421 GYVGTLEQKWNQLEKALLKMKI 442
           GYVGTLEQKWNQLEKALLKMKI
Sbjct: 421 GYVGTLEQKWNQLEKALLKMKI 442

BLAST of CsGy5G001520 vs. NCBI nr
Match: XP_008437446.1 (PREDICTED: methionine gamma-lyase-like [Cucumis melo] >KAA0042648.1 methionine gamma-lyase-like [Cucumis melo var. makuwa] >TYK06049.1 methionine gamma-lyase-like [Cucumis melo var. makuwa])

HSP 1 Score: 843 bits (2177), Expect = 1.47e-307
Identity = 427/442 (96.61%), Postives = 437/442 (98.87%), Query Frame = 0

Query: 1   MITE-VRAHDTSLPCKNNNNFSYDTEPTSITRLSSWPMGELDPAAALAMASPEFGEHGGV 60
           MIT+ V+AHDTSLPCK NNNFSYDT+PTSITRL+SWPMG++DPAAALAMA PEFGEHGGV
Sbjct: 1   MITDQVKAHDTSLPCK-NNNFSYDTKPTSITRLTSWPMGKVDPAAALAMARPEFGEHGGV 60

Query: 61  NMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAY 120
           NMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAY
Sbjct: 61  NMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAY 120

Query: 121 CTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVSKITTTFVDVRNHEMV 180
           CTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVSKITTTFVDVRNHEMV
Sbjct: 121 CTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVSKITTTFVDVRNHEMV 180

Query: 181 RDAMVEGKTKVLYFETVANPTLTVANIPELSRMAHQKGVTVVVDNTFSPIIISPTRLGAD 240
           RDAMVEGKTKVLYFETVANPTLTVANIPELSRMAH+KGVTVVVDNTFSP+IISP RLGAD
Sbjct: 181 RDAMVEGKTKVLYFETVANPTLTVANIPELSRMAHEKGVTVVVDNTFSPMIISPARLGAD 240

Query: 241 VVVHSCSKFISGGADIIAGAVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPH 300
           VVVHSCSKFISGGADIIAGAVCGSK+LVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPH
Sbjct: 241 VVVHSCSKFISGGADIIAGAVCGSKDLVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPH 300

Query: 301 LSLRMKEHSNRALVFAQRMKNLGLKVIYPGLNDHPDHQLLGSIANKDYGYGGVLCIDMGT 360
           L LRMKEHSNRALVFAQRMKNLGLKVIYPGLNDHPDHQLLGSIANKDYGYGGVLCIDMGT
Sbjct: 301 LGLRMKEHSNRALVFAQRMKNLGLKVIYPGLNDHPDHQLLGSIANKDYGYGGVLCIDMGT 360

Query: 361 EERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMS 420
           EERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMS
Sbjct: 361 EERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMS 420

Query: 421 VGYVGTLEQKWNQLEKALLKMK 441
           VGYVGTLEQKW+QLEKALLKMK
Sbjct: 421 VGYVGTLEQKWSQLEKALLKMK 441

BLAST of CsGy5G001520 vs. NCBI nr
Match: XP_038875936.1 (methionine gamma-lyase-like [Benincasa hispida])

HSP 1 Score: 795 bits (2052), Expect = 1.58e-288
Identity = 403/444 (90.77%), Postives = 420/444 (94.59%), Query Frame = 0

Query: 1   MITEVRAHDTSLPCKN--NNNFSYDTEPTSITRLSSWPMGELDPAAALAMASPEFGEHGG 60
           M TEV+AHDTSLPC N  N  FSY T+ ++   ++SWPMG+ DPAA LAMA PEFGEHGG
Sbjct: 1   MTTEVKAHDTSLPCWNIDNKKFSYQTK-SATPMVTSWPMGDEDPAATLAMARPEFGEHGG 60

Query: 61  VNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAA 120
           VNMSIENSITFTVMEAETMR MFEGELGPERDFFIYSRHFNPTV+ALSRQMAALEGTEAA
Sbjct: 61  VNMSIENSITFTVMEAETMRMMFEGELGPERDFFIYSRHFNPTVLALSRQMAALEGTEAA 120

Query: 121 YCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVSKITTTFVDVRNHEM 180
           YCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRV  ITTTFVDV++HEM
Sbjct: 121 YCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDVKDHEM 180

Query: 181 VRDAMVEGKTKVLYFETVANPTLTVANIPELSRMAHQKGVTVVVDNTFSPIIISPTRLGA 240
           VRDAMVEGKTKVLYFETVANPTLTVAN+PELSRMAH+KGVTVVVDNTFSP+IISP RLGA
Sbjct: 181 VRDAMVEGKTKVLYFETVANPTLTVANVPELSRMAHEKGVTVVVDNTFSPMIISPARLGA 240

Query: 241 DVVVHSCSKFISGGADIIAGAVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASELSQRIP 300
           DVVVHSCSKFISGGADIIAGAVCGSK LVNSMMDLHQGTLMLLGPTMNAKVASE+SQRIP
Sbjct: 241 DVVVHSCSKFISGGADIIAGAVCGSKNLVNSMMDLHQGTLMLLGPTMNAKVASEISQRIP 300

Query: 301 HLSLRMKEHSNRALVFAQRMKNLGLKVIYPGLNDHPDHQLLGSIANKDYGYGGVLCIDMG 360
           HL LRMKEHS+RALVFAQRMKNLGLKV YPGLNDHPDHQLL  IANKDYGYGGVLCIDMG
Sbjct: 301 HLGLRMKEHSHRALVFAQRMKNLGLKVTYPGLNDHPDHQLLSYIANKDYGYGGVLCIDMG 360

Query: 361 TEERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRM 420
           TEERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELN +EKELAGISPGLIRM
Sbjct: 361 TEERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNSKEKELAGISPGLIRM 420

Query: 421 SVGYVGTLEQKWNQLEKALLKMKI 442
           SVGYVGTLEQKW+QLEKALLKMKI
Sbjct: 421 SVGYVGTLEQKWSQLEKALLKMKI 443

BLAST of CsGy5G001520 vs. NCBI nr
Match: KAG7017043.1 (Methionine gamma-lyase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 742 bits (1915), Expect = 8.99e-268
Identity = 372/407 (91.40%), Postives = 392/407 (96.31%), Query Frame = 0

Query: 36  PMGELDPAAALAMASPEFGEHGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYS 95
           P GE DPAA+LA A PEFGEHGGVN+SIENSITFTVMEAETMR+MFEGELGPE+DFFIYS
Sbjct: 30  PEGEEDPAASLAAARPEFGEHGGVNLSIENSITFTVMEAETMRKMFEGELGPEQDFFIYS 89

Query: 96  RHFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHAL 155
           RHFNPTVMAL RQMAA+EGTEAAYCTSSGMAA+SSVLLQLVSSGGHIVASRTLYGGTHAL
Sbjct: 90  RHFNPTVMALGRQMAAIEGTEAAYCTSSGMAAISSVLLQLVSSGGHIVASRTLYGGTHAL 149

Query: 156 LSHFLPRVSKITTTFVDVRNHEMVRDAMVEGKTKVLYFETVANPTLTVANIPELSRMAHQ 215
           LSHFLPRV  ITT+FVDV++H  VRDAMV+ KTKVLYFETVANPTLTVANIPELSRMAH+
Sbjct: 150 LSHFLPRVCNITTSFVDVKDHAKVRDAMVKDKTKVLYFETVANPTLTVANIPELSRMAHE 209

Query: 216 KGVTVVVDNTFSPIIISPTRLGADVVVHSCSKFISGGADIIAGAVCGSKELVNSMMDLHQ 275
           KGVTVVVDNTFSP+IISPTRLGADVVVHSCSKFISGGADIIAGAVCGSK+LVNSMMDLHQ
Sbjct: 210 KGVTVVVDNTFSPMIISPTRLGADVVVHSCSKFISGGADIIAGAVCGSKKLVNSMMDLHQ 269

Query: 276 GTLMLLGPTMNAKVASELSQRIPHLSLRMKEHSNRALVFAQRMKNLGLKVIYPGLNDHPD 335
           GTLMLLGPTMNAKVASELSQR+PHL+LRMKEHS+RALVFAQRMKNLGLKVIYPGL DHPD
Sbjct: 270 GTLMLLGPTMNAKVASELSQRVPHLALRMKEHSHRALVFAQRMKNLGLKVIYPGLKDHPD 329

Query: 336 HQLLGSIANKDYGYGGVLCIDMGTEERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGS 395
           H LL SIANKDYGYGGVLCIDMGTEERANRLMNLLQNHTHFGL+AVSLG+FETLMSCSGS
Sbjct: 330 HHLLSSIANKDYGYGGVLCIDMGTEERANRLMNLLQNHTHFGLLAVSLGYFETLMSCSGS 389

Query: 396 STSSELNGEEKELAGISPGLIRMSVGYVGTLEQKWNQLEKALLKMKI 442
           STSSE+N EEKELAGISPGLIRMSVGYVGTLEQKW+QLEKALLKM I
Sbjct: 390 STSSEMNVEEKELAGISPGLIRMSVGYVGTLEQKWSQLEKALLKMNI 436

BLAST of CsGy5G001520 vs. NCBI nr
Match: XP_023520565.1 (methionine gamma-lyase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 738 bits (1905), Expect = 2.89e-266
Identity = 371/407 (91.15%), Postives = 391/407 (96.07%), Query Frame = 0

Query: 36  PMGELDPAAALAMASPEFGEHGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYS 95
           P GE DPAA+LA A PEFGEHGGVN+SIENSITFTVMEAE MR+MFEGELG E+DFFIYS
Sbjct: 29  PEGEEDPAASLAAARPEFGEHGGVNLSIENSITFTVMEAEMMRKMFEGELGLEQDFFIYS 88

Query: 96  RHFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHAL 155
           RHFNPTVMAL R MAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHAL
Sbjct: 89  RHFNPTVMALGRAMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHAL 148

Query: 156 LSHFLPRVSKITTTFVDVRNHEMVRDAMVEGKTKVLYFETVANPTLTVANIPELSRMAHQ 215
           LSHFLPRV  ITT+FVDV++H MVRDAMV+GKTKVLYFETVANPTLTVAN+PELSRMAH+
Sbjct: 149 LSHFLPRVCNITTSFVDVKDHAMVRDAMVKGKTKVLYFETVANPTLTVANVPELSRMAHE 208

Query: 216 KGVTVVVDNTFSPIIISPTRLGADVVVHSCSKFISGGADIIAGAVCGSKELVNSMMDLHQ 275
           KGVTVVVDNTFSP+IISPTRLGADVVVHSCSKFISGGADIIAGAVCGSK+LVNSMMDLHQ
Sbjct: 209 KGVTVVVDNTFSPMIISPTRLGADVVVHSCSKFISGGADIIAGAVCGSKKLVNSMMDLHQ 268

Query: 276 GTLMLLGPTMNAKVASELSQRIPHLSLRMKEHSNRALVFAQRMKNLGLKVIYPGLNDHPD 335
           GTLMLLGPTMNAKVASELSQR+PHL+LRMKEHS+RALVFAQRMKNLGLKVIYPGL DHPD
Sbjct: 269 GTLMLLGPTMNAKVASELSQRVPHLALRMKEHSHRALVFAQRMKNLGLKVIYPGLKDHPD 328

Query: 336 HQLLGSIANKDYGYGGVLCIDMGTEERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGS 395
           H LL SIANKDYGYGGVLCIDMGTEERANRLMNLLQNHTHFGL+AVSLG+FETLMSCSGS
Sbjct: 329 HHLLSSIANKDYGYGGVLCIDMGTEERANRLMNLLQNHTHFGLLAVSLGYFETLMSCSGS 388

Query: 396 STSSELNGEEKELAGISPGLIRMSVGYVGTLEQKWNQLEKALLKMKI 442
           STSSE+N EEKELAGISPGLIRMSVGYVGTLEQKW+QLEKALLK+ I
Sbjct: 389 STSSEMNVEEKELAGISPGLIRMSVGYVGTLEQKWSQLEKALLKLNI 435

BLAST of CsGy5G001520 vs. ExPASy TrEMBL
Match: A0A0A0KQL7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G148520 PE=3 SV=1)

HSP 1 Score: 874 bits (2258), Expect = 0.0
Identity = 442/442 (100.00%), Postives = 442/442 (100.00%), Query Frame = 0

Query: 1   MITEVRAHDTSLPCKNNNNFSYDTEPTSITRLSSWPMGELDPAAALAMASPEFGEHGGVN 60
           MITEVRAHDTSLPCKNNNNFSYDTEPTSITRLSSWPMGELDPAAALAMASPEFGEHGGVN
Sbjct: 1   MITEVRAHDTSLPCKNNNNFSYDTEPTSITRLSSWPMGELDPAAALAMASPEFGEHGGVN 60

Query: 61  MSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAYC 120
           MSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAYC
Sbjct: 61  MSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAYC 120

Query: 121 TSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVSKITTTFVDVRNHEMVR 180
           TSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVSKITTTFVDVRNHEMVR
Sbjct: 121 TSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVSKITTTFVDVRNHEMVR 180

Query: 181 DAMVEGKTKVLYFETVANPTLTVANIPELSRMAHQKGVTVVVDNTFSPIIISPTRLGADV 240
           DAMVEGKTKVLYFETVANPTLTVANIPELSRMAHQKGVTVVVDNTFSPIIISPTRLGADV
Sbjct: 181 DAMVEGKTKVLYFETVANPTLTVANIPELSRMAHQKGVTVVVDNTFSPIIISPTRLGADV 240

Query: 241 VVHSCSKFISGGADIIAGAVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPHL 300
           VVHSCSKFISGGADIIAGAVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPHL
Sbjct: 241 VVHSCSKFISGGADIIAGAVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPHL 300

Query: 301 SLRMKEHSNRALVFAQRMKNLGLKVIYPGLNDHPDHQLLGSIANKDYGYGGVLCIDMGTE 360
           SLRMKEHSNRALVFAQRMKNLGLKVIYPGLNDHPDHQLLGSIANKDYGYGGVLCIDMGTE
Sbjct: 301 SLRMKEHSNRALVFAQRMKNLGLKVIYPGLNDHPDHQLLGSIANKDYGYGGVLCIDMGTE 360

Query: 361 ERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMSV 420
           ERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMSV
Sbjct: 361 ERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMSV 420

Query: 421 GYVGTLEQKWNQLEKALLKMKI 442
           GYVGTLEQKWNQLEKALLKMKI
Sbjct: 421 GYVGTLEQKWNQLEKALLKMKI 442

BLAST of CsGy5G001520 vs. ExPASy TrEMBL
Match: A0A5A7TIP4 (Methionine gamma-lyase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001500 PE=3 SV=1)

HSP 1 Score: 843 bits (2177), Expect = 7.13e-308
Identity = 427/442 (96.61%), Postives = 437/442 (98.87%), Query Frame = 0

Query: 1   MITE-VRAHDTSLPCKNNNNFSYDTEPTSITRLSSWPMGELDPAAALAMASPEFGEHGGV 60
           MIT+ V+AHDTSLPCK NNNFSYDT+PTSITRL+SWPMG++DPAAALAMA PEFGEHGGV
Sbjct: 1   MITDQVKAHDTSLPCK-NNNFSYDTKPTSITRLTSWPMGKVDPAAALAMARPEFGEHGGV 60

Query: 61  NMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAY 120
           NMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAY
Sbjct: 61  NMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAY 120

Query: 121 CTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVSKITTTFVDVRNHEMV 180
           CTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVSKITTTFVDVRNHEMV
Sbjct: 121 CTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVSKITTTFVDVRNHEMV 180

Query: 181 RDAMVEGKTKVLYFETVANPTLTVANIPELSRMAHQKGVTVVVDNTFSPIIISPTRLGAD 240
           RDAMVEGKTKVLYFETVANPTLTVANIPELSRMAH+KGVTVVVDNTFSP+IISP RLGAD
Sbjct: 181 RDAMVEGKTKVLYFETVANPTLTVANIPELSRMAHEKGVTVVVDNTFSPMIISPARLGAD 240

Query: 241 VVVHSCSKFISGGADIIAGAVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPH 300
           VVVHSCSKFISGGADIIAGAVCGSK+LVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPH
Sbjct: 241 VVVHSCSKFISGGADIIAGAVCGSKDLVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPH 300

Query: 301 LSLRMKEHSNRALVFAQRMKNLGLKVIYPGLNDHPDHQLLGSIANKDYGYGGVLCIDMGT 360
           L LRMKEHSNRALVFAQRMKNLGLKVIYPGLNDHPDHQLLGSIANKDYGYGGVLCIDMGT
Sbjct: 301 LGLRMKEHSNRALVFAQRMKNLGLKVIYPGLNDHPDHQLLGSIANKDYGYGGVLCIDMGT 360

Query: 361 EERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMS 420
           EERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMS
Sbjct: 361 EERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMS 420

Query: 421 VGYVGTLEQKWNQLEKALLKMK 441
           VGYVGTLEQKW+QLEKALLKMK
Sbjct: 421 VGYVGTLEQKWSQLEKALLKMK 441

BLAST of CsGy5G001520 vs. ExPASy TrEMBL
Match: A0A1S3AU15 (methionine gamma-lyase-like OS=Cucumis melo OX=3656 GN=LOC103482860 PE=3 SV=1)

HSP 1 Score: 843 bits (2177), Expect = 7.13e-308
Identity = 427/442 (96.61%), Postives = 437/442 (98.87%), Query Frame = 0

Query: 1   MITE-VRAHDTSLPCKNNNNFSYDTEPTSITRLSSWPMGELDPAAALAMASPEFGEHGGV 60
           MIT+ V+AHDTSLPCK NNNFSYDT+PTSITRL+SWPMG++DPAAALAMA PEFGEHGGV
Sbjct: 1   MITDQVKAHDTSLPCK-NNNFSYDTKPTSITRLTSWPMGKVDPAAALAMARPEFGEHGGV 60

Query: 61  NMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAY 120
           NMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAY
Sbjct: 61  NMSIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAY 120

Query: 121 CTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVSKITTTFVDVRNHEMV 180
           CTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVSKITTTFVDVRNHEMV
Sbjct: 121 CTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVSKITTTFVDVRNHEMV 180

Query: 181 RDAMVEGKTKVLYFETVANPTLTVANIPELSRMAHQKGVTVVVDNTFSPIIISPTRLGAD 240
           RDAMVEGKTKVLYFETVANPTLTVANIPELSRMAH+KGVTVVVDNTFSP+IISP RLGAD
Sbjct: 181 RDAMVEGKTKVLYFETVANPTLTVANIPELSRMAHEKGVTVVVDNTFSPMIISPARLGAD 240

Query: 241 VVVHSCSKFISGGADIIAGAVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPH 300
           VVVHSCSKFISGGADIIAGAVCGSK+LVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPH
Sbjct: 241 VVVHSCSKFISGGADIIAGAVCGSKDLVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPH 300

Query: 301 LSLRMKEHSNRALVFAQRMKNLGLKVIYPGLNDHPDHQLLGSIANKDYGYGGVLCIDMGT 360
           L LRMKEHSNRALVFAQRMKNLGLKVIYPGLNDHPDHQLLGSIANKDYGYGGVLCIDMGT
Sbjct: 301 LGLRMKEHSNRALVFAQRMKNLGLKVIYPGLNDHPDHQLLGSIANKDYGYGGVLCIDMGT 360

Query: 361 EERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMS 420
           EERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMS
Sbjct: 361 EERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMS 420

Query: 421 VGYVGTLEQKWNQLEKALLKMK 441
           VGYVGTLEQKW+QLEKALLKMK
Sbjct: 421 VGYVGTLEQKWSQLEKALLKMK 441

BLAST of CsGy5G001520 vs. ExPASy TrEMBL
Match: A0A6J1I2P5 (methionine gamma-lyase-like OS=Cucurbita maxima OX=3661 GN=LOC111469051 PE=3 SV=1)

HSP 1 Score: 728 bits (1879), Expect = 1.10e-262
Identity = 365/407 (89.68%), Postives = 387/407 (95.09%), Query Frame = 0

Query: 36  PMGELDPAAALAMASPEFGEHGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYS 95
           P  E DPAA+LA   PEFGEHGGVN+SIENSI+FTVMEAETMR+MFEGELGPE+D FIYS
Sbjct: 25  PEEEEDPAASLAAVRPEFGEHGGVNLSIENSISFTVMEAETMRKMFEGELGPEQDCFIYS 84

Query: 96  RHFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHAL 155
           RHFNPTVMAL RQMAALEGTEAAYCTSSGMAAVSSVLLQL+SSGGHIVASRTLYGGTHAL
Sbjct: 85  RHFNPTVMALGRQMAALEGTEAAYCTSSGMAAVSSVLLQLLSSGGHIVASRTLYGGTHAL 144

Query: 156 LSHFLPRVSKITTTFVDVRNHEMVRDAMVEGKTKVLYFETVANPTLTVANIPELSRMAHQ 215
           LSHFLPRV  ITT+FVDV++H MVRDAMV+GKTKVLYFETVANPTLTVANIPELSRMAH+
Sbjct: 145 LSHFLPRVCNITTSFVDVKDHAMVRDAMVKGKTKVLYFETVANPTLTVANIPELSRMAHE 204

Query: 216 KGVTVVVDNTFSPIIISPTRLGADVVVHSCSKFISGGADIIAGAVCGSKELVNSMMDLHQ 275
           KGVTVVVDNTFSP+IISPTRLGADVVVHSCSKFISGGADIIAGAVCG K+LVNSMMDLHQ
Sbjct: 205 KGVTVVVDNTFSPMIISPTRLGADVVVHSCSKFISGGADIIAGAVCGKKKLVNSMMDLHQ 264

Query: 276 GTLMLLGPTMNAKVASELSQRIPHLSLRMKEHSNRALVFAQRMKNLGLKVIYPGLNDHPD 335
           GTLMLLGPTMNAKVASELSQR+PHL+LRMKEHS+R LVFAQRMKNLGLKVIYPGL DHPD
Sbjct: 265 GTLMLLGPTMNAKVASELSQRVPHLALRMKEHSHRTLVFAQRMKNLGLKVIYPGLKDHPD 324

Query: 336 HQLLGSIANKDYGYGGVLCIDMGTEERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGS 395
           H LL SIANKDYGYGGVLCIDMGTEERANRLMNLLQNHTHFG +AVSLG+FETL+SCSGS
Sbjct: 325 HHLLSSIANKDYGYGGVLCIDMGTEERANRLMNLLQNHTHFGFLAVSLGYFETLISCSGS 384

Query: 396 STSSELNGEEKELAGISPGLIRMSVGYVGTLEQKWNQLEKALLKMKI 442
           STSSE+N EEKELAGISPGLIRMSV YVGTLEQKW+QLEKALLK+ I
Sbjct: 385 STSSEMNVEEKELAGISPGLIRMSVAYVGTLEQKWSQLEKALLKLNI 431

BLAST of CsGy5G001520 vs. ExPASy TrEMBL
Match: A0A6J1E0P6 (methionine gamma-lyase OS=Momordica charantia OX=3673 GN=LOC111026086 PE=3 SV=1)

HSP 1 Score: 695 bits (1794), Expect = 1.50e-249
Identity = 357/443 (80.59%), Postives = 387/443 (87.36%), Query Frame = 0

Query: 2   ITEVRAHDTSLPCKNNNNFSYDTEPTSITRLSSWPMGELDPAAALAMASPEFGEHGGVNM 61
           + EV A     PC N        E     + ++    E DPAAALA+A  EFGEHGGVNM
Sbjct: 1   MAEVEARPIPHPCWNEKKKLIKYEKAKSPKPATKSATEEDPAAALALAEHEFGEHGGVNM 60

Query: 62  SIENSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAYCT 121
           S+E SITFTVMEAETMRRMFEGE GPE + FIYSRHFNPTVMALSRQMAALEGTEAAYCT
Sbjct: 61  SVEASITFTVMEAETMRRMFEGEEGPEGECFIYSRHFNPTVMALSRQMAALEGTEAAYCT 120

Query: 122 SSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVSKITTTFVDVRNHEMVRD 181
           SSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRV  ITTTFVD+++H MV +
Sbjct: 121 SSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDIKDHGMVAN 180

Query: 182 AMVEGKTKVLYFETVANPTLTVANIPELSRMAHQKGVTVVVDNTFSPIIISPTRLGADVV 241
           AM+EGKTKVLYFETVANPTLTVA+IPELSRMAH+KGVTVVVDNTF+P++ISP RLGADVV
Sbjct: 181 AMLEGKTKVLYFETVANPTLTVADIPELSRMAHEKGVTVVVDNTFAPMVISPVRLGADVV 240

Query: 242 VHSCSKFISGGADIIAGAVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPHLS 301
           VHS SKFISGGADIIAGAVCGSK+LV+SMMDL QGTLMLLGPTMN KVA ELSQR+PHL 
Sbjct: 241 VHSSSKFISGGADIIAGAVCGSKKLVHSMMDLQQGTLMLLGPTMNPKVACELSQRVPHLG 300

Query: 302 LRMKEHSNRALVFAQRMKNLGLKVIYPGLNDHPDHQLLGSIANKDYGYG--GVLCIDMGT 361
           LRMKEH +RAL+FAQR++NLG KVIYPGL DHPDH L+ SIAN  YGYG  GVLCIDM T
Sbjct: 301 LRMKEHCHRALIFAQRIQNLGFKVIYPGLKDHPDHHLINSIANTSYGYGYGGVLCIDMET 360

Query: 362 EERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMS 421
           EERANRLMNLLQNHT FGLMAVSLGFFETLMSCSGSSTSSELN EEKELAGISPGL+R+S
Sbjct: 361 EERANRLMNLLQNHTKFGLMAVSLGFFETLMSCSGSSTSSELNAEEKELAGISPGLVRIS 420

Query: 422 VGYVGTLEQKWNQLEKALLKMKI 442
           VGYVGTLEQKW+QLE+ LLKMK+
Sbjct: 421 VGYVGTLEQKWSQLERGLLKMKL 443

BLAST of CsGy5G001520 vs. TAIR 10
Match: AT1G64660.1 (methionine gamma-lyase )

HSP 1 Score: 615.1 bits (1585), Expect = 4.3e-176
Identity = 305/426 (71.60%), Postives = 361/426 (84.74%), Query Frame = 0

Query: 15  KNNNNFSYDTEPTSITRLSSWPMGELDPAAALAMASPEFGEHGGVNMSIENSITFTVMEA 74
           K  N+   +     + + S+  + + DPAAA+A    EFGEHGGVNMSIE S TFTVME 
Sbjct: 16  KKRNDRDDEDGDALVAKKSALAVCDADPAAAIANIRHEFGEHGGVNMSIEASATFTVMEP 75

Query: 75  ETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQ 134
           +TMRRMF GELGP+ DF++YSRHFNPTV+ LSRQMAALEGT+AAYCTSSGM+A+SSV+LQ
Sbjct: 76  DTMRRMFTGELGPDNDFYVYSRHFNPTVLNLSRQMAALEGTQAAYCTSSGMSAISSVMLQ 135

Query: 135 LVSSGGHIVASRTLYGGTHALLSHFLPRVSKITTTFVDVRNHEMVRDAMVEGKTKVLYFE 194
           L SSGGH+VA+ TLYGGTHALLSHFLPR   ITT+FVD+ +H  V +A+VEG+T+VLYFE
Sbjct: 136 LCSSGGHVVAASTLYGGTHALLSHFLPRTCNITTSFVDITDHGAVANAIVEGRTQVLYFE 195

Query: 195 TVANPTLTVANIPELSRMAHQKGVTVVVDNTFSPIIISPTRLGADVVVHSCSKFISGGAD 254
           +VANPTLTVA+IPELSRMAH+KGVTVVVDNTF+P+++SP +LGADVVVHS SKFISGGAD
Sbjct: 196 SVANPTLTVADIPELSRMAHEKGVTVVVDNTFAPMVLSPAKLGADVVVHSISKFISGGAD 255

Query: 255 IIAGAVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASELSQRIPHLSLRMKEHSNRALVF 314
           IIAGAVCGS+ LV  MMDL  G+LMLLGPTMNAKVA ELS+RIPHL LRM+EHS+RA V+
Sbjct: 256 IIAGAVCGSENLVKEMMDLRGGSLMLLGPTMNAKVAFELSERIPHLGLRMREHSHRAQVY 315

Query: 315 AQRMKNLGLKVIYPGLNDHPDHQLLGSIANKDYGYGGVLCIDMGTEERANRLMNLLQNHT 374
           A+RM++LG+KVIYPGL  HP H+L   + N+DYGYGG+L IDM TEE+AN+LM  LQN T
Sbjct: 316 AERMRDLGMKVIYPGLETHPQHKLFKGMVNRDYGYGGLLSIDMETEEKANKLMAYLQNAT 375

Query: 375 HFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPGLIRMSVGYVGTLEQKWNQLE 434
            FG MAVSLG++ETLMSCSGSSTSSEL+  +KE AGISPGL+RMSVGYVGTLEQKW Q E
Sbjct: 376 QFGFMAVSLGYYETLMSCSGSSTSSELDPSQKEAAGISPGLVRMSVGYVGTLEQKWTQFE 435

Query: 435 KALLKM 441
           KA L+M
Sbjct: 436 KAFLRM 441

BLAST of CsGy5G001520 vs. TAIR 10
Match: AT3G01120.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 163.7 bits (413), Expect = 3.4e-40
Identity = 110/334 (32.93%), Postives = 183/334 (54.79%), Query Frame = 0

Query: 92  FIYSRHFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGG 151
           F Y R+ NPT + L  +++ALEG E+    +SGM A + +LL LV +GGHIV +   Y  
Sbjct: 224 FEYGRYGNPTTVVLEDKISALEGAESTLVMASGMCASTVMLLALVPAGGHIVTTTDCYRK 283

Query: 152 THALLSHFLPRVSKITTTFVDVRNHEMVRDAMVEGKTKVLYFETVANPTLTVANIPELSR 211
           T   + +FLP++  IT T +D  +   +  A+ E K  + + E+  NP L   +I  +S+
Sbjct: 284 TRIFMENFLPKLG-ITVTVIDPADIAGLEAAVNEFKVSLFFTESPTNPFLRCVDIELVSK 343

Query: 212 MAHQKGVTVVVDNTF-SPIIISPTRLGADVVVHSCSKFISGGADIIAGAVCGSKELVNSM 271
           + H++G  V +D TF +P+      LGAD+VVHS +K+I G  D++AG +CGS +LV+ +
Sbjct: 344 ICHKRGTLVCIDGTFATPLNQKALALGADLVVHSATKYIGGHNDVLAGCICGSLKLVSEI 403

Query: 272 MDLHQGTLMLLGPTMNAKVASELSQRIPHLSLRMKEHSNRALVFAQRMK--NLGLKVIYP 331
            +LH     +LG T+N   A  + + +  L LR+++ ++ A   A+ ++       V YP
Sbjct: 404 RNLHH----VLGGTLNPNAAYLIIRGMKTLHLRVQQQNSTAFRMAEILEAHPKVSHVYYP 463

Query: 332 GLNDHPDHQLLGSIANKDYGYGGVLCIDM-GTEERANRLMNLLQNHTHFGLMAVSLGFFE 391
           GL  HP+H+L      +  G+GGV+  ++ G  E   + ++ L+       +A S G  E
Sbjct: 464 GLPSHPEHEL---AKRQMTGFGGVVSFEIDGDIETTIKFVDSLK----IPYIAPSFGGCE 523

Query: 392 TLMSCSGSSTSSELNGEEKELAGISPGLIRMSVG 422
           +++      +  +L  EE+   GI   L+R S G
Sbjct: 524 SIVDQPAIMSYWDLPQEERLKYGIKDNLVRFSFG 545

BLAST of CsGy5G001520 vs. TAIR 10
Match: AT1G33320.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 144.8 bits (364), Expect = 1.6e-34
Identity = 103/336 (30.65%), Postives = 180/336 (53.57%), Query Frame = 0

Query: 90  DFFIYSRHFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLY 149
           D + Y+R+ NPT MAL  +++ LEG E+    +SGM A + +LL LV + GHIVA++  Y
Sbjct: 72  DLYEYARYGNPTTMALEEKISVLEGAESTLVMASGMYASNVMLLALVPTNGHIVATKDCY 131

Query: 150 GGTHALLSHFLPRVSKITTTFVDVRNHEMVRDAMVEGKTKVLYFETVANPTLTVANIPEL 209
             T   + +FL ++  IT TF+D  +   ++  +   +  + + E+  NP L   +I  +
Sbjct: 132 KETRIFMENFLTKLG-ITVTFIDSDDIAGLQTLVNNHEVSLFFTESPTNPFLRCVDIKLV 191

Query: 210 SRMAHQKGVTVVVDNTF-SPIIISPTRLGADVVVHSCSKFISGGADIIAGAVCGSKELVN 269
           S++ H++G  V +D T  +PI      LGAD+V HS +K+I G  D +AG++ GS ELV+
Sbjct: 192 SKICHRRGTLVCIDATIATPINQKTLALGADLVHHSATKYIGGHNDFLAGSISGSMELVS 251

Query: 270 SMMDLHQGTLMLLGPTMNAKVASELSQRIPHLSLRMKEHSNRALVFAQRMK--NLGLKVI 329
            + +LH+    LLG T+N   A  L + +  + LR+++ ++  +  AQ ++      +V 
Sbjct: 252 KIRNLHK----LLGGTLNPNAAYLLIRGMKTMHLRVRQQNSTGMKMAQVLEAHPKVSRVY 311

Query: 330 YPGLNDHPDHQLLGSIANKDYGYGGVLCIDM-GTEERANRLMNLLQNHTHFGLMAVSLGF 389
           Y GL  HP+H +      +  G GG++  ++ G  +   + ++ L+       +A S G 
Sbjct: 312 YLGLPSHPEHLI---AKRQMTGIGGLISFEIDGDLKTTIKFIDALK----IPYLAASFGG 371

Query: 390 FETLMSCSGSSTSSELNGEEKELAGISPGLIRMSVG 422
            E+L+    +    ++  EE+   G    L+R S G
Sbjct: 372 CESLVDQLATGI-WDIPREERLKDGFQDNLVRFSFG 394

BLAST of CsGy5G001520 vs. TAIR 10
Match: AT3G57050.1 (cystathionine beta-lyase )

HSP 1 Score: 142.5 bits (358), Expect = 8.1e-34
Identity = 119/367 (32.43%), Postives = 192/367 (52.32%), Query Frame = 0

Query: 65  NSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAYCTSSG 124
           ++++  + +  T ++    E GP    + Y+R  NPT  AL   +A L+  + A+C +SG
Sbjct: 102 DAMSTPLYQTATFKQPSAIENGP----YDYTRSGNPTRDALESLLAKLDKADRAFCFTSG 161

Query: 125 MAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPR----VSKITTTFVDVRNHEMVR 184
           MAA+S+V   L+ +G  IVA   +YGG+  LLS  +PR    V ++ TT +D    E+  
Sbjct: 162 MAALSAV-THLIKNGEEIVAGDDVYGGSDRLLSQVVPRSGVVVKRVNTTKLD----EVA- 221

Query: 185 DAMVEGKTKVLYFETVANPTLTVANIPELSRMAHQKGVTVVVDNT-FSPIIISPTRLGAD 244
            A +  +TK+++ E+  NP   +++I ++S MAH +G  V+VDN+  SP++  P  LGAD
Sbjct: 222 -AAIGPQTKLVWLESPTNPRQQISDIRKISEMAHAQGALVLVDNSIMSPVLSRPLELGAD 281

Query: 245 VVVHSCSKFISGGADIIAG--AVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASELSQR- 304
           +V+HS +KFI+G +D++AG  AV G K L   +  L       L P         L  R 
Sbjct: 282 IVMHSATKFIAGHSDVMAGVLAVKGEK-LAKEVYFLQNSEGSGLAP-----FDCWLCLRG 341

Query: 305 IPHLSLRMKEHSNRALVFAQRMKN--LGLKVIYPGLNDHPDHQLLGSIANKDYGYGGVLC 364
           I  ++LR+++    A   A  + +     KV Y GL DHP H L  S A    G G V  
Sbjct: 342 IKTMALRIEKQQENARKIAMYLSSHPRVKKVYYAGLPDHPGHHLHFSQAK---GAGSVFS 401

Query: 365 IDMGTEERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPG 422
              G+   +  L+      T +  +AVS G  ++L+S     + + +  E +E  G++  
Sbjct: 402 FITGSVALSKHLVET----TKYFSIAVSFGSVKSLISMPCFMSHASIPAEVREARGLTED 444

BLAST of CsGy5G001520 vs. TAIR 10
Match: AT3G57050.2 (cystathionine beta-lyase )

HSP 1 Score: 142.5 bits (358), Expect = 8.1e-34
Identity = 119/367 (32.43%), Postives = 192/367 (52.32%), Query Frame = 0

Query: 65  NSITFTVMEAETMRRMFEGELGPERDFFIYSRHFNPTVMALSRQMAALEGTEAAYCTSSG 124
           ++++  + +  T ++    E GP    + Y+R  NPT  AL   +A L+  + A+C +SG
Sbjct: 87  DAMSTPLYQTATFKQPSAIENGP----YDYTRSGNPTRDALESLLAKLDKADRAFCFTSG 146

Query: 125 MAAVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPR----VSKITTTFVDVRNHEMVR 184
           MAA+S+V   L+ +G  IVA   +YGG+  LLS  +PR    V ++ TT +D    E+  
Sbjct: 147 MAALSAV-THLIKNGEEIVAGDDVYGGSDRLLSQVVPRSGVVVKRVNTTKLD----EVA- 206

Query: 185 DAMVEGKTKVLYFETVANPTLTVANIPELSRMAHQKGVTVVVDNT-FSPIIISPTRLGAD 244
            A +  +TK+++ E+  NP   +++I ++S MAH +G  V+VDN+  SP++  P  LGAD
Sbjct: 207 -AAIGPQTKLVWLESPTNPRQQISDIRKISEMAHAQGALVLVDNSIMSPVLSRPLELGAD 266

Query: 245 VVVHSCSKFISGGADIIAG--AVCGSKELVNSMMDLHQGTLMLLGPTMNAKVASELSQR- 304
           +V+HS +KFI+G +D++AG  AV G K L   +  L       L P         L  R 
Sbjct: 267 IVMHSATKFIAGHSDVMAGVLAVKGEK-LAKEVYFLQNSEGSGLAP-----FDCWLCLRG 326

Query: 305 IPHLSLRMKEHSNRALVFAQRMKN--LGLKVIYPGLNDHPDHQLLGSIANKDYGYGGVLC 364
           I  ++LR+++    A   A  + +     KV Y GL DHP H L  S A    G G V  
Sbjct: 327 IKTMALRIEKQQENARKIAMYLSSHPRVKKVYYAGLPDHPGHHLHFSQAK---GAGSVFS 386

Query: 365 IDMGTEERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGSSTSSELNGEEKELAGISPG 422
              G+   +  L+      T +  +AVS G  ++L+S     + + +  E +E  G++  
Sbjct: 387 FITGSVALSKHLVET----TKYFSIAVSFGSVKSLISMPCFMSHASIPAEVREARGLTED 429

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SGU96.0e-17571.60Methionine gamma-lyase OS=Arabidopsis thaliana OX=3702 GN=MGL PE=1 SV=1[more]
Q8L0X47.1e-5137.76L-methionine gamma-lyase OS=Fusobacterium nucleatum subsp. polymorphum OX=76857 ... [more]
Q8RDT48.7e-4936.57L-methionine gamma-lyase OS=Fusobacterium nucleatum subsp. nucleatum (strain ATC... [more]
Q7MX716.2e-4737.81L-methionine gamma-lyase OS=Porphyromonas gingivalis (strain ATCC BAA-308 / W83)... [more]
Q73KL71.7e-4434.68L-methionine gamma-lyase OS=Treponema denticola (strain ATCC 35405 / DSM 14222 /... [more]
Match NameE-valueIdentityDescription
XP_004143943.30.0100.00methionine gamma-lyase [Cucumis sativus] >KGN49981.1 hypothetical protein Csa_00... [more]
XP_008437446.11.47e-30796.61PREDICTED: methionine gamma-lyase-like [Cucumis melo] >KAA0042648.1 methionine g... [more]
XP_038875936.11.58e-28890.77methionine gamma-lyase-like [Benincasa hispida][more]
KAG7017043.18.99e-26891.40Methionine gamma-lyase, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023520565.12.89e-26691.15methionine gamma-lyase-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A0A0KQL70.0100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G148520 PE=3 SV=1[more]
A0A5A7TIP47.13e-30896.61Methionine gamma-lyase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3AU157.13e-30896.61methionine gamma-lyase-like OS=Cucumis melo OX=3656 GN=LOC103482860 PE=3 SV=1[more]
A0A6J1I2P51.10e-26289.68methionine gamma-lyase-like OS=Cucurbita maxima OX=3661 GN=LOC111469051 PE=3 SV=... [more]
A0A6J1E0P61.50e-24980.59methionine gamma-lyase OS=Momordica charantia OX=3673 GN=LOC111026086 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G64660.14.3e-17671.60methionine gamma-lyase [more]
AT3G01120.13.4e-4032.93Pyridoxal phosphate (PLP)-dependent transferases superfamily protein [more]
AT1G33320.11.6e-3430.65Pyridoxal phosphate (PLP)-dependent transferases superfamily protein [more]
AT3G57050.18.1e-3432.43cystathionine beta-lyase [more]
AT3G57050.28.1e-3432.43cystathionine beta-lyase [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015421Pyridoxal phosphate-dependent transferase, major domainGENE3D3.40.640.10coord: 44..297
e-value: 9.0E-67
score: 227.0
IPR000277Cys/Met metabolism, pyridoxal phosphate-dependent enzymePIRSFPIRSF001434CGScoord: 37..441
e-value: 6.0E-101
score: 335.9
IPR000277Cys/Met metabolism, pyridoxal phosphate-dependent enzymePFAMPF01053Cys_Met_Meta_PPcoord: 55..422
e-value: 8.2E-83
score: 278.0
IPR000277Cys/Met metabolism, pyridoxal phosphate-dependent enzymePANTHERPTHR11808TRANS-SULFURATION ENZYME FAMILY MEMBERcoord: 40..441
IPR015422Pyridoxal phosphate-dependent transferase, small domainGENE3D3.90.1150.10Aspartate Aminotransferase, domain 1coord: 301..441
e-value: 7.2E-24
score: 86.5
NoneNo IPR availablePIRSRPIRSR001434-2PIRSR001434-2coord: 95..401
e-value: 1.2E-78
score: 262.2
NoneNo IPR availablePANTHERPTHR11808:SF76METHIONINE GAMMA-LYASE-LIKEcoord: 40..441
IPR015424Pyridoxal phosphate-dependent transferaseSUPERFAMILY53383PLP-dependent transferasescoord: 55..437

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy5G001520.2CsGy5G001520.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019343 cysteine biosynthetic process via cystathionine
biological_process GO:0019346 transsulfuration
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004123 cystathionine gamma-lyase activity
molecular_function GO:0018826 methionine gamma-lyase activity
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0003824 catalytic activity