CsGy5G000410 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy5G000410
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionPectinesterase
LocationGy14Chr5: 229397 .. 234566 (-)
RNA-Seq ExpressionCsGy5G000410
SyntenyCsGy5G000410
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCTCTCTAGCTATGTTTTAGTATAAGGAACATAAATTGAAATAATGGGAGAGGCAAGAAGGTAGCAAGACATCCCCACATAACACACACGTATTCATACACACATTAAAGTAAAATATTTTTCCTTTTTTTCTCTTTTTCAAAGAACGAGTAGAAAGTTTTAGAAGCAAAACAAAAGGACCCAACCCCACTTTATTATTACAAACATATAGTGGAATAATAATAATAATCATCTTCATCCTTGCAAGTATATATGAAGCCCTTTTCTTATTTAAACTCACTCCCACCATTATTCCCTCCCCATGCACACAAATAACCGACCATGAATAACAATAACAGCTACAACAAGCTCCACACCTCCCACTCCGCCAACACCACTCCGGCAGCTTCCTTCCGCCTCTCCGGCAATGATCCCCCTCCACCCTCCTCCTCCCTCCGTCGTCGCTCTCGTATCCTAATCATCTCTTTCATGTCCGTTCTCCTTATTGCTGCTTCAGTCGTTTCCGCGGCTATCCTTGTTCGATCACATATGACATCTAGCCAACCCCATTTCCCTCACAACAAGCCTTCCCAAGCCATCTCCCGCGCGTGTAGCCACACGCTCTACCCATCTCTTTGCCTCTCCTCTCTTCTATCCTTCCCCGGCGCACAATCGGCCGACGTTCATGATCTTGTTCATATCTCTCTCAACTTAACTCTTCAGCATCTCACCAAAGCTCTTTACTCCACATCTCAAATCCCTGTCTTACAAATCTCTAAGGACCCTTTGGCTCATTCCGCTTATGAAGATTGTATGGAGCTTTTAAACGACGCCATTGATGCCTTTTCTCTCTCTCTTTTCTCTAAGGATGCTTCCAATCATGATATCATGACGTGGCTTAGTGCTGCATTGACTTACCACGACACGTGTACGGCTGGATTTCAAGATGTGGCTGACTTGGGTGTTAAGGATGAGGTGGAGGCCAAGTTGAGTGATTTGTCGGAGATGATTAGTAATTCTTTGGCCATATTTTCTGGGTTTGGTGGTGGGGATTTGCCAGTGGAGAATAGAAAGCGGCGGCGGTTGATGGAGAGTAGTACGACGTCGTGGGCGGCGGAGAATGGTGGTGATCATGAGGGGTTTCCTGCGTGGTTGAGTGGGAAGGATCGGCGGCTTTTGGCTGCTCCCCTATCGACGATTCAAGCGGATATTGTGGTTGCTAAGGATGGTTCTGGCAAATTCAAGACGGTGGCTGAGGCCATTGAGGCCGCTCCGTCGTCGAGTGGCCGCCGGATCATCATCTACATTAAGGCTGGAAAGTAAGAATTAACTATACCCTTTTTTTAAATACTCTTAGGAAATCAACTCTCAGCTCAGTTATTGTTTATTTTGGCATAACTATACTTTTCTCTAAATATTCTCATATACATCTTTTTTACTATTTAAATTATCACTGCACTCTAAACTATCATACTTTTAGCACGTGTCGACTTATGAGTGGACACTAGTGTTTAGTCAGAGATACACATAGTAATTATTTTTCTAAAAGTATAGTGTTTCAATGTCTACAAGAGACTATATATACATATATTTGCCCTTTGATTAATACATTTTTGCTAGTAATGTAATAGGTGGTTTTAAAAACTTTAGTTTGAAGAAGATAGCTAGAAATCTAGGGGTATAGTTAGTTGGATTGTTAGAGAGTAGTGGATGATGATTTTCTTTATAATTAATTATTAAGTTAATTTTCTTAATCTTTTTTACCTTTCAAAATTCGAATGATTATTAGTGAAACTTCTTTTTCTTGGGAAAGTGGAGAAAGAAAATGGTTGTGATTAAGCCATAACTAACCAAGATTAATCAAAAGGAAGCAGGGAAAGAAGGAAAAAGAAAAGTTTTATTGACTTCAGTGTCATAATCATGCAAAGCTGTTACTTTACCAATTCCTAAAGTCCGTAGTTTTTTTTTAATATCATGATAATAATTATACAATCAAAACAATATTTTATATGTCCCCTCACAATAATTAATTTAAGTCCTTTTCAATTTAGTTGCTTTCATAATAATAACTCTTAATAACAACCAAAAATTTAAAATCAATAAAATTCAACACATGAATAAATAAAATAACCCATCATTAGAATAATTATCAGTATATATATATTTAAAGGGAAACATTTTTAGGAGAATTTATTTATTTAATTAAAATTAAAATTAGGATGTTTTGATAATAATAAATTGAGAAATGGTGTGGAGTAATAGCAGGGAGATAAATGTAAATTTGTAGGTACGAAGAGGAGAATTTGAAGGTGGGAAGGAAGAAAACAAACTTGATGTTCGTGGGAGATGGGAAGGGCATAACTGTCATTTCAGGTGGCAAAAGTGTTTATGACAAAGTTACCACTTTTCGCACCGCCACCTTTGGTATATACTCTCTCACCCTCCTTTCCTCTTTTCTTTTCTTTTCATTTTTATTCCTTTTTACTCAAAATTCTCTCCATACATAATTAATTAAAATAGAGTTAAAAAATCTTAAATTGTTTTTTTTGGAAAAAGTTAATAACAAATTAGTAGTATAAGCTTAATTTAAAATTAATTAAATAATTAATTTGATTTTTGAATAGAAATGAAGGCTTTTAATTCTAACACACCGGATATCTAGGGCTTGGAATTATGGTCGTCCGGCCTTAGTACTAAAAGTGGCCATACTATATCACCTTTTTCTTCTTAAACTTTCTTTTTGTCTATTCAATTTTCAAAATCATATCATCCCCAAATTAAGAAGTAGATTTAAAAATTTTCATATAAAATATATGAATATCAAAATGGACCCATATGCATGTAGATTTGTTAGAATATTAGATATTTAACGAGTATTTAATACGTACAATAATACAGTAAGTTTAAATCCTTGTGGTAGTTTTGAGAGTTTAGAGAACGAAATAATATTAATTAAAAAAATAATTTCTATAGTAATAATAATAATTATTATCATAATTTTTGTTTAAAAAAATTGGGGTTATATTTCAAGATAGTGACAAATTAAGCTACTTTGGTAGCATTTAATGGGCTATAAAAATAATTGTTTGATTTATATCCAAATAATCATAAGAGAAACAGTAGATTCCAGCTGTGTTTTGTCAAGGAGTCTTCATCATTAATTATTTTTGTAGGAAAAAACACACACCATTCATATTTTTTTAATAATTATATGCTATGCTAGTGTATACGTTTACTACTAAACTGAGGCTTACAAATTCAATCTTTGACGTAACATTTTCACATGGCATATTATTAAATATATATCATCATAAATATTCTTTTATTACTAAGGGTATTATAAAACTTTGATCATCTTTCAAAAGATAATAAAGTATCATATGATAGTCGTAATTAGGCAAGTTGTATTAAAGGGTTATTATTATTATTATTCATATTATAAGAAGATTCTATAGTAGGTGTTTTAAAAATCTTTTTGTTATCTCTATAAGAAAGATGATGTGTCTTTCATTATTATATAATATATTGTGCCTATCATAATTAATTTGTAAAGTATTCCACTCTAATAAATTGTTATAATATTAATAATTTTTGGAAGAAGAAGAAGAAGAAGATGAGAATAATAATAAAAAGAATGAAATGGAATGGAATATCCCAATTGTTCATGATGATTTAGAAAAGTCATAGTAGTATTATTCCCATGTTTCTTGCATCATGCTTACTTCCACAAGTTGGTAAAAAGTTTTCTTTTTTCAAATGGGAATCTAATATGGTCCAAATAGAGATGCCAAAAGCTTACTCTCCATGGAGATAAGGATAGATGTTAGAAATTAATTATTGACTTTTATTTCAAAAGTTCTCCATCTCTCATCACATAATCTATGGTTCATGATTGTGTCATGAGGACTTAAACATTAAACATTATTAAGATTTAAATGGTATGTCAATGTTTAATATGTTAATTTTTTTTTTGTTGTAAACAGCGGGTTCAGGAACAAACATAATTCTACGAGACATGACGTTTGAGAATACGGCAGGACCAAGTAAGCACCAAGCAGTGGCATTACGGTTGAGTGCAGACCACGCGGTGGTATACCATTGCAACATCATTGGATATCAAGACACTCTCTACGTGCACTCTAACCGTCAATTCTTCCGGGAGTGCGACATCTACGGAACAATAGACTTCATCTTCGGCAACGCTGTAGTGGTTTTCCAAAGTTGCAACATCTACGCTCGAAAGCCAATGGCAGGACAAAAAAACACGATAACAGCACAAGACCGAAAGGACCCGAACCAAAACACAGGAATCTCAATCCACGCTTGCAAGATCGTAGCCACCGGCGACCTTGAAGCGTCCAAGGGTTCTTTCCCCACATTTCTAGGTCGACCTTGGAAACTATACTCTCGAGTGGTGTACATGGTGTCTTCCATGGGAGACCATATCCATCCACGTGGGTGGCTTGAGTGGCAAGGCTCTTTTGCTCTTGACACTCTTTATTACGGTGAGTATATGAATTCCGGGCCGGGGGCCGCCGTGGGGCAGCGGGTCAAGTGGCCGGGTTACCGAGTCATTACTTCCACGGTGGAAGCCTCCAAGTTTACCGTCGGCCAATTTATTTACGGTTCTTCTTGGTTGCCTTCTACCGGAGTCTCCTTTTCTGCTGGATTATCTACTAACTAAAAATGCATATATATATATATATATATATATATATATATATATATATATGTAGTATATTATATGATATGATATCATATGTATTTAAATAATTTCTTTAAAAATTTGTTTTTGTTTTTTTGGAGGTTATTGTTATTTTGTTTTTAGGATATGGTTTATGGTGACAGTGTATAGTCTCATTCCTTTGATTATGCTCTTCCAAAATAATATGGGCCATATACATCAAACCTATGACAAATATTTGGATAGGATATGGTCATTTATATATATATATATATATTATATACACATACACATATATATGGTTATAATTTTGATTGTATTTGTTGTATCAACTTCATATATTTTGATCAGAATAAAATTTGTTTTCTCTTTTCCTCTTCATTATTTTCATAAATGTGTAATATTCCTCTGCACGTATTGATTCGGAGTAGAATAAGAAAGACAGTGGTGCGTTTTAGGCGTTGGTCAATTTACCTAAAGAAATAAATAGGTGTCTTTGTTCTTAC

mRNA sequence

ATCTCTCTAGCTATGTTTTAGTATAAGGAACATAAATTGAAATAATGGGAGAGGCAAGAAGGTAGCAAGACATCCCCACATAACACACACGTATTCATACACACATTAAAGTAAAATATTTTTCCTTTTTTTCTCTTTTTCAAAGAACGAGTAGAAAGTTTTAGAAGCAAAACAAAAGGACCCAACCCCACTTTATTATTACAAACATATAGTGGAATAATAATAATAATCATCTTCATCCTTGCAAGTATATATGAAGCCCTTTTCTTATTTAAACTCACTCCCACCATTATTCCCTCCCCATGCACACAAATAACCGACCATGAATAACAATAACAGCTACAACAAGCTCCACACCTCCCACTCCGCCAACACCACTCCGGCAGCTTCCTTCCGCCTCTCCGGCAATGATCCCCCTCCACCCTCCTCCTCCCTCCGTCGTCGCTCTCGTATCCTAATCATCTCTTTCATGTCCGTTCTCCTTATTGCTGCTTCAGTCGTTTCCGCGGCTATCCTTGTTCGATCACATATGACATCTAGCCAACCCCATTTCCCTCACAACAAGCCTTCCCAAGCCATCTCCCGCGCGTGTAGCCACACGCTCTACCCATCTCTTTGCCTCTCCTCTCTTCTATCCTTCCCCGGCGCACAATCGGCCGACGTTCATGATCTTGTTCATATCTCTCTCAACTTAACTCTTCAGCATCTCACCAAAGCTCTTTACTCCACATCTCAAATCCCTGTCTTACAAATCTCTAAGGACCCTTTGGCTCATTCCGCTTATGAAGATTGTATGGAGCTTTTAAACGACGCCATTGATGCCTTTTCTCTCTCTCTTTTCTCTAAGGATGCTTCCAATCATGATATCATGACGTGGCTTAGTGCTGCATTGACTTACCACGACACGTGTACGGCTGGATTTCAAGATGTGGCTGACTTGGGTGTTAAGGATGAGGTGGAGGCCAAGTTGAGTGATTTGTCGGAGATGATTAGTAATTCTTTGGCCATATTTTCTGGGTTTGGTGGTGGGGATTTGCCAGTGGAGAATAGAAAGCGGCGGCGGTTGATGGAGAGTAGTACGACGTCGTGGGCGGCGGAGAATGGTGGTGATCATGAGGGGTTTCCTGCGTGGTTGAGTGGGAAGGATCGGCGGCTTTTGGCTGCTCCCCTATCGACGATTCAAGCGGATATTGTGGTTGCTAAGGATGGTTCTGGCAAATTCAAGACGGTGGCTGAGGCCATTGAGGCCGCTCCGTCGTCGAGTGGCCGCCGGATCATCATCTACATTAAGGCTGGAAAGTACGAAGAGGAGAATTTGAAGGTGGGAAGGAAGAAAACAAACTTGATGTTCGTGGGAGATGGGAAGGGCATAACTGTCATTTCAGGTGGCAAAAGTGTTTATGACAAAGTTACCACTTTTCGCACCGCCACCTTTGCGGGTTCAGGAACAAACATAATTCTACGAGACATGACGTTTGAGAATACGGCAGGACCAAGTAAGCACCAAGCAGTGGCATTACGGTTGAGTGCAGACCACGCGGTGGTATACCATTGCAACATCATTGGATATCAAGACACTCTCTACGTGCACTCTAACCGTCAATTCTTCCGGGAGTGCGACATCTACGGAACAATAGACTTCATCTTCGGCAACGCTGTAGTGGTTTTCCAAAGTTGCAACATCTACGCTCGAAAGCCAATGGCAGGACAAAAAAACACGATAACAGCACAAGACCGAAAGGACCCGAACCAAAACACAGGAATCTCAATCCACGCTTGCAAGATCGTAGCCACCGGCGACCTTGAAGCGTCCAAGGGTTCTTTCCCCACATTTCTAGGTCGACCTTGGAAACTATACTCTCGAGTGGTGTACATGGTGTCTTCCATGGGAGACCATATCCATCCACGTGGGTGGCTTGAGTGGCAAGGCTCTTTTGCTCTTGACACTCTTTATTACGGTGAGTATATGAATTCCGGGCCGGGGGCCGCCGTGGGGCAGCGGGTCAAGTGGCCGGGTTACCGAGTCATTACTTCCACGGTGGAAGCCTCCAAGTTTACCGTCGGCCAATTTATTTACGGTTCTTCTTGGTTGCCTTCTACCGGAGTCTCCTTTTCTGCTGGATTATCTACTAACTAAAAATGCATATATATATATATATATATATATATATATATATATATATATGTAGTATATTATATGATATGATATCATATGTATTTAAATAATTTCTTTAAAAATTTGTTTTTGTTTTTTTGGAGGTTATTGTTATTTTGTTTTTAGGATATGGTTTATGGTGACAGTGTATAGTCTCATTCCTTTGATTATGCTCTTCCAAAATAATATGGGCCATATACATCAAACCTATGACAAATATTTGGATAGGATATGGTCATTTATATATATATATATATATTATATACACATACACATATATATGGTTATAATTTTGATTGTATTTGTTGTATCAACTTCATATATTTTGATCAGAATAAAATTTGTTTTCTCTTTTCCTCTTCATTATTTTCATAAATGTGTAATATTCCTCTGCACGTATTGATTCGGAGTAGAATAAGAAAGACAGTGGTGCGTTTTAGGCGTTGGTCAATTTACCTAAAGAAATAAATAGGTGTCTTTGTTCTTAC

Coding sequence (CDS)

ATGAATAACAATAACAGCTACAACAAGCTCCACACCTCCCACTCCGCCAACACCACTCCGGCAGCTTCCTTCCGCCTCTCCGGCAATGATCCCCCTCCACCCTCCTCCTCCCTCCGTCGTCGCTCTCGTATCCTAATCATCTCTTTCATGTCCGTTCTCCTTATTGCTGCTTCAGTCGTTTCCGCGGCTATCCTTGTTCGATCACATATGACATCTAGCCAACCCCATTTCCCTCACAACAAGCCTTCCCAAGCCATCTCCCGCGCGTGTAGCCACACGCTCTACCCATCTCTTTGCCTCTCCTCTCTTCTATCCTTCCCCGGCGCACAATCGGCCGACGTTCATGATCTTGTTCATATCTCTCTCAACTTAACTCTTCAGCATCTCACCAAAGCTCTTTACTCCACATCTCAAATCCCTGTCTTACAAATCTCTAAGGACCCTTTGGCTCATTCCGCTTATGAAGATTGTATGGAGCTTTTAAACGACGCCATTGATGCCTTTTCTCTCTCTCTTTTCTCTAAGGATGCTTCCAATCATGATATCATGACGTGGCTTAGTGCTGCATTGACTTACCACGACACGTGTACGGCTGGATTTCAAGATGTGGCTGACTTGGGTGTTAAGGATGAGGTGGAGGCCAAGTTGAGTGATTTGTCGGAGATGATTAGTAATTCTTTGGCCATATTTTCTGGGTTTGGTGGTGGGGATTTGCCAGTGGAGAATAGAAAGCGGCGGCGGTTGATGGAGAGTAGTACGACGTCGTGGGCGGCGGAGAATGGTGGTGATCATGAGGGGTTTCCTGCGTGGTTGAGTGGGAAGGATCGGCGGCTTTTGGCTGCTCCCCTATCGACGATTCAAGCGGATATTGTGGTTGCTAAGGATGGTTCTGGCAAATTCAAGACGGTGGCTGAGGCCATTGAGGCCGCTCCGTCGTCGAGTGGCCGCCGGATCATCATCTACATTAAGGCTGGAAAGTACGAAGAGGAGAATTTGAAGGTGGGAAGGAAGAAAACAAACTTGATGTTCGTGGGAGATGGGAAGGGCATAACTGTCATTTCAGGTGGCAAAAGTGTTTATGACAAAGTTACCACTTTTCGCACCGCCACCTTTGCGGGTTCAGGAACAAACATAATTCTACGAGACATGACGTTTGAGAATACGGCAGGACCAAGTAAGCACCAAGCAGTGGCATTACGGTTGAGTGCAGACCACGCGGTGGTATACCATTGCAACATCATTGGATATCAAGACACTCTCTACGTGCACTCTAACCGTCAATTCTTCCGGGAGTGCGACATCTACGGAACAATAGACTTCATCTTCGGCAACGCTGTAGTGGTTTTCCAAAGTTGCAACATCTACGCTCGAAAGCCAATGGCAGGACAAAAAAACACGATAACAGCACAAGACCGAAAGGACCCGAACCAAAACACAGGAATCTCAATCCACGCTTGCAAGATCGTAGCCACCGGCGACCTTGAAGCGTCCAAGGGTTCTTTCCCCACATTTCTAGGTCGACCTTGGAAACTATACTCTCGAGTGGTGTACATGGTGTCTTCCATGGGAGACCATATCCATCCACGTGGGTGGCTTGAGTGGCAAGGCTCTTTTGCTCTTGACACTCTTTATTACGGTGAGTATATGAATTCCGGGCCGGGGGCCGCCGTGGGGCAGCGGGTCAAGTGGCCGGGTTACCGAGTCATTACTTCCACGGTGGAAGCCTCCAAGTTTACCGTCGGCCAATTTATTTACGGTTCTTCTTGGTTGCCTTCTACCGGAGTCTCCTTTTCTGCTGGATTATCTACTAACTAA

Protein sequence

MNNNNSYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVVSAAILVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHISLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNHDIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPVENRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGLSTN*
Homology
BLAST of CsGy5G000410 vs. ExPASy Swiss-Prot
Match: Q9FK05 (Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3702 GN=PME61 PE=2 SV=1)

HSP 1 Score: 703.4 bits (1814), Expect = 2.3e-201
Identity = 365/594 (61.45%), Postives = 438/594 (73.74%), Query Frame = 0

Query: 20  PAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVVSAAILVRSHMTSSQPHFPH 79
           P+     +  DP      L+++++  +I F   +L+   VV   I        S    P 
Sbjct: 8   PSGPSNPNQKDPATSLPELQKKTKTKLILFTLAVLV-VGVVCFGIFAGIRAVDSGKTEPK 67

Query: 80  --NKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHISLNLTLQHLTKALYSTS 139
              KP+QAISR CS +LYP+LC+ +LL FPG+ +AD ++L+HIS N TLQ  +KALY++S
Sbjct: 68  LTRKPTQAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSS 127

Query: 140 QIPVLQISKDPLAHSAYEDCMELLNDAIDAF-----SLSLFSKDASNHDIMTWLSAALTY 199
            I   Q+   P   SAY+ C+ELL+D++DA      S+ + S D S+ D+MTWLS+A+T 
Sbjct: 128 TITYTQM--PPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHSDVMTWLSSAMTN 187

Query: 200 HDTCTAGFQDVADLG--VKDEVEAKLSDLSEMISNSLAIFSG--FGGGDLPVENRKRRRL 259
           HDTCT GF ++   G  VKD+V   + DLSEM+SN LAIF+G       +PV N   R+L
Sbjct: 188 HDTCTDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVN--NRKL 247

Query: 260 MESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKTVAEAIE 319
           + +  T          E  P WL  +DR LL  P S IQADI V+KDGSG FKT+AEAI+
Sbjct: 248 LGTEET----------EELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIK 307

Query: 320 AAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRT 379
            AP  S RR +IY+KAG+YEEENLKVGRKKTNLMF+GDGKG TVI+GGKS+ D +TTF T
Sbjct: 308 KAPEHSSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHT 367

Query: 380 ATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQF 439
           ATFA +G   I+RDMTFEN AGP+KHQAVALR+  DHAVVY CNIIGYQD LYVHSNRQF
Sbjct: 368 ATFAATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQF 427

Query: 440 FRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKI 499
           FREC+IYGT+DFIFGNA V+ QSCNIYARKPMA QK TITAQ+RKDPNQNTGISIHACK+
Sbjct: 428 FRECEIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKL 487

Query: 500 VATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEY 559
           +AT DLEASKGS+PT+LGRPWKLYSRVVYM+S MGDHI PRGWLEW G FALD+LYYGEY
Sbjct: 488 LATPDLEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEY 547

Query: 560 MNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGLS 603
           MN G G+ +GQRVKWPGY VITSTVEASKFTV QFI GSSWLPSTGVSF +GLS
Sbjct: 548 MNKGLGSGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGLS 586

BLAST of CsGy5G000410 vs. ExPASy Swiss-Prot
Match: Q9M3B0 (Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana OX=3702 GN=PME34 PE=2 SV=1)

HSP 1 Score: 684.5 bits (1765), Expect = 1.1e-195
Identity = 347/599 (57.93%), Postives = 439/599 (73.29%), Query Frame = 0

Query: 13  SHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISF-MSVLLIAASVVSAAILVRSHMT 72
           S + +TT A           P +++ R + ++++ S  +++ LI A+ + A +  R  + 
Sbjct: 14  SVTTSTTTAPILNQVSTSEQPENNNRRSKKKLVVSSIVLAISLILAAAIFAGVRSRLKLN 73

Query: 73  SSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSA-DVHDLVHISLNLTLQHLT 132
            S P     KPSQAIS+AC  T +P LC+ SL+ FPG+ +A    DL+H+++N+TL H +
Sbjct: 74  QSVPGLA-RKPSQAISKACELTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHFS 133

Query: 133 KALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFS---LSLFSKDASNHDIMTWLS 192
            ALYS++ +    +   P A SAY+ C+ELL+D++DA S    S+ S  A   D+ TWLS
Sbjct: 134 HALYSSASLSF--VDMPPRARSAYDSCVELLDDSVDALSRALSSVVSSSAKPQDVTTWLS 193

Query: 193 AALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGD----LPVENR 252
           AALT HDTCT GF  V D GVKD + A L +LSE++SN LAIFS    GD    +P++NR
Sbjct: 194 AALTNHDTCTEGFDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNR 253

Query: 253 KRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKTV 312
           +   + E              E FP W+  K+R +L  P+S IQADI+V+KDG+G  KT+
Sbjct: 254 RLLGVEER------------EEKFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTI 313

Query: 313 AEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKV 372
           +EAI+ AP +S RRIIIY+KAG+YEE NLKVGRKK NLMFVGDGKG TVISGGKS++D +
Sbjct: 314 SEAIKKAPQNSTRRIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNI 373

Query: 373 TTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVH 432
           TTF TA+FA +G   I RD+TFEN AGP+KHQAVALR+ ADHAV+Y CNIIGYQDTLYVH
Sbjct: 374 TTFHTASFAATGAGFIARDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVH 433

Query: 433 SNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISI 492
           SNRQFFRECDIYGT+DFIFGNA VV Q+C+IYARKPM  QKNTITAQ+RKDPNQNTGISI
Sbjct: 434 SNRQFFRECDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISI 493

Query: 493 HACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTL 552
           HA +++A  DL+A+ GS  T+LGRPWKL+SR VYM+S +G H+H RGWLEW  +FALDTL
Sbjct: 494 HASRVLAASDLQATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTL 553

Query: 553 YYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGLS 603
           YYGEY+NSGPG+ +GQRV WPGYRVI ST EA++FTV +FIYGSSWLPSTGVSF AGLS
Sbjct: 554 YYGEYLNSGPGSGLGQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGLS 597

BLAST of CsGy5G000410 vs. ExPASy Swiss-Prot
Match: O49006 (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME3 PE=1 SV=2)

HSP 1 Score: 469.5 bits (1207), Expect = 5.5e-131
Identity = 260/589 (44.14%), Postives = 364/589 (61.80%), Query Frame = 0

Query: 37  SLRRRSRILIISFMSVLLIAASV--VSAAILVRSHMTSSQPHFPHNKPSQAISR-ACSHT 96
           + ++  +++++S    LL  A+V  +SA     +   +  P       S A+ R +CS T
Sbjct: 13  NFKKNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSP------SSHAVLRSSCSST 72

Query: 97  LYPSLCLSSLLSFPGAQSADVHDLVHISLNLTLQHLTKALYSTSQIPVLQISKDPLAHSA 156
            YP LC+S++++  G +     D++  S+NLT+  +    ++  ++   +    P   +A
Sbjct: 73  RYPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTA 132

Query: 157 YEDCMELLNDAIDAF-----SLSLF--SKDASNH--DIMTWLSAALTYHDTCTAGF-QDV 216
             DC+E +++ +D        L L+   K    H  D+ T +S+A+T  +TC  GF  D 
Sbjct: 133 LHDCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDD 192

Query: 217 ADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDL-------PVENRKRRRLMESSTTSW 276
           AD  V+  +      +  M SN+LA+       D+        + +  R+   E+  T+ 
Sbjct: 193 ADKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTV 252

Query: 277 AAENGG----DHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKTVAEAIEAAPS 336
           A +  G    D EG+P WLS  DRRLL    S ++AD  VA DGSG FKTVA A+ AAP 
Sbjct: 253 AVDIAGAGELDSEGWPTWLSAGDRRLLQG--SGVKADATVAADGSGTFKTVAAAVAAAPE 312

Query: 337 SSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATFA 396
           +S +R +I+IKAG Y  EN++V +KK N+MF+GDG+  T+I+G ++V D  TTF +AT A
Sbjct: 313 NSNKRYVIHIKAGVY-RENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVA 372

Query: 397 GSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFREC 456
             G   + RD+TF+NTAGPSKHQAVALR+ +D +  Y+C+++ YQDTLYVHSNRQFF +C
Sbjct: 373 AVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKC 432

Query: 457 DIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVATG 516
            I GT+DFIFGNA VV Q C+I+AR+P +GQKN +TAQ R DPNQNTGI I  C+I AT 
Sbjct: 433 LIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATS 492

Query: 517 DLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSG 576
           DL++ KGSFPT+LGRPWK YS+ V M S++ D I P GW EW G+FAL+TL Y EY N+G
Sbjct: 493 DLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTG 552

Query: 577 PGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 602
            GA    RVKW G++VIT+  EA K+T GQFI G  WL STG  FS GL
Sbjct: 553 AGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

BLAST of CsGy5G000410 vs. ExPASy Swiss-Prot
Match: Q42534 (Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2)

HSP 1 Score: 468.8 bits (1205), Expect = 9.5e-131
Identity = 263/580 (45.34%), Postives = 350/580 (60.34%), Query Frame = 0

Query: 36  SSLRRRSRILIISFMSVLLIAASVVSAAILVRSHMTSSQPHFPHNKPSQAISRA-CSHTL 95
           S  +   ++++ S    LL+ AS+V  A    ++   +Q     +  S AI ++ CS TL
Sbjct: 12  SDFKNNKKLILSSAAIALLLLASIVGIA-ATTTNQNKNQKITTLSSTSHAILKSVCSSTL 71

Query: 96  YPSLCLSSLLSFPGAQSADVHDLVHISLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAY 155
           YP LC S++ +  G +     +++  SLNLT + +    ++  ++   +    P   +A 
Sbjct: 72  YPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTAL 131

Query: 156 EDCMELLNDAIDAFSLSL-------FSKDASNH--DIMTWLSAALTYHDTCTAGFQ-DVA 215
            DC+E +++ +D   +++         K    H  D+ T +S+A+T   TC  GF  D A
Sbjct: 132 HDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDA 191

Query: 216 DLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLP-VENRKRRRLMESSTTSWAAENGG- 275
           D  V+  +      +  M SN+LA+       D+   E R +     ++      E  G 
Sbjct: 192 DRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKEVTGD 251

Query: 276 -DHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKTVAEAIEAAPSSSGRRIIIY 335
            D +G+P WLS  DRRLL    STI+AD  VA DGSG F TVA A+ AAP  S +R +I+
Sbjct: 252 LDSDGWPKWLSVGDRRLLQG--STIKADATVADDGSGDFTTVAAAVAAAPEKSNKRFVIH 311

Query: 336 IKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATFAGSGTNIILR 395
           IKAG Y  EN++V +KKTN+MF+GDG+G T+I+G ++V D  TTF +AT A  G   + R
Sbjct: 312 IKAGVY-RENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVGERFLAR 371

Query: 396 DMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFRECDIYGTIDFI 455
           D+TF+NTAGPSKHQAVALR+ +D +  Y C++  YQDTLYVHSNRQFF +C I GT+DFI
Sbjct: 372 DITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCHITGTVDFI 431

Query: 456 FGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVATGDLEASKGSF 515
           FGNA  V Q C+I AR+P +GQKN +TAQ R DPNQNTGI I  C+I  T DL A KG+F
Sbjct: 432 FGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLLAVKGTF 491

Query: 516 PTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAAVGQRV 575
           PT+LGRPWK YSR V M S + D I P GW EW GSFALDTL Y EY+N G GA    RV
Sbjct: 492 PTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRGGGAGTANRV 551

Query: 576 KWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 602
           KW GY+VITS  EA  FT GQFI G  WL STG  FS  L
Sbjct: 552 KWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

BLAST of CsGy5G000410 vs. ExPASy Swiss-Prot
Match: P83948 (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)

HSP 1 Score: 465.3 bits (1196), Expect = 1.0e-129
Identity = 264/591 (44.67%), Postives = 365/591 (61.76%), Query Frame = 0

Query: 25  RLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVVSAAILVRSHMTSSQPHFPHNKPSQ 84
           +LS +      S++ ++ + L ++  + LL+ A+V+     V S   S       N+P  
Sbjct: 10  KLSESSTNQNISNIPKKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGD---NGNEPHH 69

Query: 85  AISR-ACSHTLYPSLCLSSLLSFPGAQS--ADVHDLVHISLNLTLQHLTKALYSTSQIPV 144
           AI + +CS T YP LC S++ + P A        D++ +SLN+T   +    +   ++ +
Sbjct: 70  AILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKL-L 129

Query: 145 LQISKDPLAHSAYEDCMELLNDAIDAFSLSL-------FSKDASNH--DIMTWLSAALTY 204
            + +       A  DC+E +++ +D    ++         K  S H  D+ T +SAA+T 
Sbjct: 130 KRTNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTN 189

Query: 205 HDTCTAGF-QDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV-ENRKRRRLME 264
             TC  GF  D A+  V+D +      + +M SN+LA+       D+ +      R+L+E
Sbjct: 190 QGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSNNRKLIE 249

Query: 265 SSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKTVAEAIEAA 324
            ++T          +G+PAWLS  DRRLL +  S++  ++VVA DGSG FKTVA ++ AA
Sbjct: 250 ETSTV---------DGWPAWLSTGDRRLLQS--SSVTPNVVVAADGSGNFKTVAASVAAA 309

Query: 325 PSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTAT 384
           P    +R II IKAG Y  EN++V +K  N+MF+GDG+  T+I+G ++V D  TTF++AT
Sbjct: 310 PQGGTKRYIIRIKAGVY-RENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSAT 369

Query: 385 FAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFR 444
            A  G   + RD+TF+NTAGPSKHQAVALR+ AD +  Y+C+++ YQDTLYVHSNRQFF 
Sbjct: 370 VAVVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFV 429

Query: 445 ECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVA 504
            C I GT+DFIFGNA  V Q+C+I+ARKP +GQKN +TAQ R DPNQNTGI I   +I A
Sbjct: 430 NCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGA 489

Query: 505 TGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMN 564
           T DL+  +GSFPT+LGRPWK YSR V M SS+ D IHP GW EW G+FAL+TL+YGE+ N
Sbjct: 490 TSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQN 549

Query: 565 SGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 602
           +G GA    RVKW G+RVITS  EA  FT G FI GSSWL STG  FS GL
Sbjct: 550 AGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of CsGy5G000410 vs. NCBI nr
Match: XP_011654619.1 (probable pectinesterase/pectinesterase inhibitor 61 [Cucumis sativus] >KGN49865.1 hypothetical protein Csa_000423 [Cucumis sativus])

HSP 1 Score: 1198 bits (3100), Expect = 0.0
Identity = 604/604 (100.00%), Postives = 604/604 (100.00%), Query Frame = 0

Query: 1   MNNNNSYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60
           MNNNNSYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV
Sbjct: 1   MNNNNSYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60

Query: 61  SAAILVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 120
           SAAILVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI
Sbjct: 61  SAAILVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 120

Query: 121 SLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNH 180
           SLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNH
Sbjct: 121 SLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNH 180

Query: 181 DIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240
           DIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV
Sbjct: 181 DIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240

Query: 241 ENRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKF 300
           ENRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKF
Sbjct: 241 ENRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKF 300

Query: 301 KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 360
           KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY
Sbjct: 301 KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 360

Query: 361 DKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL 420
           DKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL
Sbjct: 361 DKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL 420

Query: 421 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 480
           YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG
Sbjct: 421 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 480

Query: 481 ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 540
           ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL
Sbjct: 481 ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 540

Query: 541 DTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAG 600
           DTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAG
Sbjct: 541 DTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAG 600

Query: 601 LSTN 604
           LSTN
Sbjct: 601 LSTN 604

BLAST of CsGy5G000410 vs. NCBI nr
Match: XP_008437596.1 (PREDICTED: LOW QUALITY PROTEIN: probable pectinesterase/pectinesterase inhibitor 61 [Cucumis melo])

HSP 1 Score: 1149 bits (2971), Expect = 0.0
Identity = 581/605 (96.03%), Postives = 589/605 (97.36%), Query Frame = 0

Query: 1   MNNNNSYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60
           MNNNN+Y +LHTSHSANTTPA SFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV
Sbjct: 1   MNNNNNYKQLHTSHSANTTPATSFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60

Query: 61  SAAILVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 120
           SAAILVRS + SSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI
Sbjct: 61  SAAILVRSRIASSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 120

Query: 121 SLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNH 180
           SLNLTLQHLTKALYSTSQIP+LQISKDPLAHSAYEDCMELLNDAIDAFSLSLFS  ASNH
Sbjct: 121 SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTGASNH 180

Query: 181 DIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240
           DIMTWLSAALTYHDTCT GFQDV DLGVK+EVEAKLSDLSEMISNSLAIFSGFGGGDLPV
Sbjct: 181 DIMTWLSAALTYHDTCTDGFQDVTDLGVKNEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240

Query: 241 ENRKRRRLMESST-TSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGK 300
           ENRKRRRLMES T +SWAAENGGDHEGFP WLSGKDRRLLAAP+S IQADIVVAKDGSGK
Sbjct: 241 ENRKRRRLMESGTPSSWAAENGGDHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDGSGK 300

Query: 301 FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV 360
           FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEE LKVGRKKTNLMFVGDGKGITVISGGKSV
Sbjct: 301 FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEEILKVGRKKTNLMFVGDGKGITVISGGKSV 360

Query: 361 YDKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDT 420
           YDKVTTFRTATFAGSGT IILRDM+FENTAGPSKHQAVALRLSADHAVVY CNIIGYQDT
Sbjct: 361 YDKVTTFRTATFAGSGTGIILRDMSFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDT 420

Query: 421 LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNT 480
           LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQK TITAQDRKDPNQNT
Sbjct: 421 LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKXTITAQDRKDPNQNT 480

Query: 481 GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFA 540
           GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFA
Sbjct: 481 GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFA 540

Query: 541 LDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSA 600
           LDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSA
Sbjct: 541 LDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSA 600

Query: 601 GLSTN 604
           GLSTN
Sbjct: 601 GLSTN 605

BLAST of CsGy5G000410 vs. NCBI nr
Match: XP_038874841.1 (probable pectinesterase/pectinesterase inhibitor 61 [Benincasa hispida])

HSP 1 Score: 1072 bits (2773), Expect = 0.0
Identity = 543/604 (89.90%), Postives = 568/604 (94.04%), Query Frame = 0

Query: 4   NNSYNKLHTSHSANTTPAASFRLSGND---PPPPSSSLRRRSRILIISFMSVLLIAASVV 63
           NN+YNKLH+S SA +TPA+SF LSGND    PPPSSS RRRSRILIISF+SVLLIAAS+V
Sbjct: 3   NNNYNKLHSSLSATSTPASSFHLSGNDLPPTPPPSSSHRRRSRILIISFLSVLLIAASIV 62

Query: 64  SAAILVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 123
           SAA+LVRS + S++PHFPHNKPSQAISRACSHTLYPSLCLSSLLS P A SADVHDLVHI
Sbjct: 63  SAAVLVRSRIASNEPHFPHNKPSQAISRACSHTLYPSLCLSSLLSIPDALSADVHDLVHI 122

Query: 124 SLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNH 183
           SLNLTLQHLTKALYSTSQIP+ QIS DPLAHSAYEDCMELLNDA+DAFS+SLFS +ASNH
Sbjct: 123 SLNLTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSVSLFSTEASNH 182

Query: 184 DIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 243
           D+MTWLSAALTYHDTCT GF DVADL VK++VEAKLSDLSEMISNSLAIFSGFGGGDLPV
Sbjct: 183 DVMTWLSAALTYHDTCTDGFADVADLAVKNQVEAKLSDLSEMISNSLAIFSGFGGGDLPV 242

Query: 244 ENRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKF 303
           +NR RRRLMES T SWAAENGGD  GFP WLS KDRRLLAAP+S IQADIVVAKDGSGKF
Sbjct: 243 QNR-RRRLMESGTRSWAAENGGDG-GFPQWLSVKDRRLLAAPISAIQADIVVAKDGSGKF 302

Query: 304 KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 363
           KTVAEAI+AAPSSSGRRI+IYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV+
Sbjct: 303 KTVAEAIDAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVF 362

Query: 364 DKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL 423
           DKVTTFRTATFAGSGT IILRDM FENTAGPSKHQAVALRLSADHAVVY CNIIGYQDTL
Sbjct: 363 DKVTTFRTATFAGSGTGIILRDMIFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDTL 422

Query: 424 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 483
           YVHSNRQFFRECDIYGT+DFIFGNAVVVFQSCNIYARKPM GQKNTITAQDRKDPNQNTG
Sbjct: 423 YVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNTG 482

Query: 484 ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 543
           ISIHACKI+AT DL+ASKGSFPTFLGRPWKLYSRVVYMVS MGDHIHPRGWLEWQGSFAL
Sbjct: 483 ISIHACKIIATSDLQASKGSFPTFLGRPWKLYSRVVYMVSFMGDHIHPRGWLEWQGSFAL 542

Query: 544 DTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAG 603
           DTLYYGEY NSGPGAAVGQRVKW GYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAG
Sbjct: 543 DTLYYGEYSNSGPGAAVGQRVKWSGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAG 602

BLAST of CsGy5G000410 vs. NCBI nr
Match: XP_022922302.1 (probable pectinesterase/pectinesterase inhibitor 61 [Cucurbita moschata])

HSP 1 Score: 1014 bits (2623), Expect = 0.0
Identity = 515/604 (85.26%), Postives = 553/604 (91.56%), Query Frame = 0

Query: 4   NNSYNKLHTSHSANTTPAASFRLSGN--DPPPPSSSLRRRSRILIISFMSVLLIAASVVS 63
           NN+YN LH+SHS +T PA+SFR+S +  DP PP S    R RIL+ISF S+LLI AS VS
Sbjct: 2   NNNYNMLHSSHSGDT-PASSFRVSNDHSDPLPPPSPSSSRRRILLISFFSILLITASAVS 61

Query: 64  AAILVRSHMTSS-QPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 123
           A +LV S + +S + HFPHNKP+QAISRACS TLYPSLCLSSLLS PG+ SADVHDLVHI
Sbjct: 62  AVVLVHSRIAASTESHFPHNKPTQAISRACSLTLYPSLCLSSLLSAPGSLSADVHDLVHI 121

Query: 124 SLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNH 183
           SLNLTLQHLTKALYSTSQIP+ QIS DPLA SAYEDCMELLNDA++AFSLSLFS  A+NH
Sbjct: 122 SLNLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTTATNH 181

Query: 184 DIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 243
           D+MTWLSAALTYHDTCT GF DVAD  +KD++ AKLSDLSEMISNSLAIFSGFGG DLPV
Sbjct: 182 DVMTWLSAALTYHDTCTDGFSDVAD-PIKDQITAKLSDLSEMISNSLAIFSGFGGDDLPV 241

Query: 244 ENRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKF 303
           ENR+RRRLMES     A EN GD + FP W+SGKDRRLLAAP+S+IQADIVVAKDGSGKF
Sbjct: 242 ENRRRRRLMESDEQWLAEENNGDGD-FPRWVSGKDRRLLAAPISSIQADIVVAKDGSGKF 301

Query: 304 KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 363
           KTVAEAI AAPSSSGRRI+IYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY
Sbjct: 302 KTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 361

Query: 364 DKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL 423
           DKVTTFRTATFAGSGT +ILRDMTFENTAGP+KHQAVALRLSADHAVV+  N+IGYQDTL
Sbjct: 362 DKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHAVVFRSNVIGYQDTL 421

Query: 424 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 483
           YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPM+GQKNTITAQDRKDPNQNTG
Sbjct: 422 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMSGQKNTITAQDRKDPNQNTG 481

Query: 484 ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 543
           ISIHACKI+ATGDL+ASKGSFPT+LGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL
Sbjct: 482 ISIHACKIIATGDLQASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 541

Query: 544 DTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAG 603
           DTLYYGEYMNSGPGAAVGQRVKWPGYRVITS +EA+KFTVGQFIYGSSWLPSTGVSFSAG
Sbjct: 542 DTLYYGEYMNSGPGAAVGQRVKWPGYRVITSAIEATKFTVGQFIYGSSWLPSTGVSFSAG 601

BLAST of CsGy5G000410 vs. NCBI nr
Match: KAG6579321.1 (putative pectinesterase/pectinesterase inhibitor 61, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1014 bits (2622), Expect = 0.0
Identity = 515/604 (85.26%), Postives = 553/604 (91.56%), Query Frame = 0

Query: 4   NNSYNKLHTSHSANTTPAASFRLSGN--DPPPPSSSLRRRSRILIISFMSVLLIAASVVS 63
           NN+YNKLH+S S +T PA+SFR+S +  DP PP S    R RIL+ISF+S+ LI AS VS
Sbjct: 2   NNNYNKLHSSRSGDT-PASSFRVSNDHSDPLPPPSPSSSRRRILLISFLSIFLITASAVS 61

Query: 64  AAILVRSHMTSS-QPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 123
           A +LV S + +S + HFPHNKP+QAISRACS TLYPSLCLSSLLS PG+ SADVHDLVHI
Sbjct: 62  AVVLVHSRIAASTESHFPHNKPTQAISRACSLTLYPSLCLSSLLSAPGSLSADVHDLVHI 121

Query: 124 SLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNH 183
           SLNLTLQHLTKALYSTSQIP+ QIS DPLA SAYEDCMELLNDA++AFSLSLFS  A+NH
Sbjct: 122 SLNLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTTATNH 181

Query: 184 DIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 243
           D+MTWLSAALTYHDTCT GF DVAD  +KD++ AKLSDLSEMISNSLAIFSGFGG DLPV
Sbjct: 182 DVMTWLSAALTYHDTCTDGFSDVAD-PIKDQITAKLSDLSEMISNSLAIFSGFGGDDLPV 241

Query: 244 ENRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKF 303
           ENR+RRRLMES     A EN GD + FP W+SGKDRRLLAAP+S+IQADIVVAKDGSGKF
Sbjct: 242 ENRRRRRLMESDEHWLAEENNGDGD-FPQWVSGKDRRLLAAPISSIQADIVVAKDGSGKF 301

Query: 304 KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 363
           KTVAEAI AAPSSSGRRI+IYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY
Sbjct: 302 KTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 361

Query: 364 DKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL 423
           DKVTTFRTATFAGSGT +ILRDMTFENTAGP+KHQAVALRLSADHAVV+  N+IGYQDTL
Sbjct: 362 DKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHAVVFRSNVIGYQDTL 421

Query: 424 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 483
           YVHSNRQFFRECDIYGTIDFIFGNA VVFQSCNIYARKPM+GQKNTITAQDRKDPNQNTG
Sbjct: 422 YVHSNRQFFRECDIYGTIDFIFGNAAVVFQSCNIYARKPMSGQKNTITAQDRKDPNQNTG 481

Query: 484 ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 543
           ISIHACKI+ATGDLEASKGSFPT+LGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL
Sbjct: 482 ISIHACKIIATGDLEASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 541

Query: 544 DTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAG 603
           DTLYYGEYMNSGPGAAVGQRVKWPGYRVITST+EA+KFTVGQFIYGSSWLPSTGVSFSAG
Sbjct: 542 DTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTIEATKFTVGQFIYGSSWLPSTGVSFSAG 601

BLAST of CsGy5G000410 vs. ExPASy TrEMBL
Match: A0A0A0KJG1 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G139430 PE=3 SV=1)

HSP 1 Score: 1198 bits (3100), Expect = 0.0
Identity = 604/604 (100.00%), Postives = 604/604 (100.00%), Query Frame = 0

Query: 1   MNNNNSYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60
           MNNNNSYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV
Sbjct: 1   MNNNNSYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60

Query: 61  SAAILVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 120
           SAAILVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI
Sbjct: 61  SAAILVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 120

Query: 121 SLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNH 180
           SLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNH
Sbjct: 121 SLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNH 180

Query: 181 DIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240
           DIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV
Sbjct: 181 DIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240

Query: 241 ENRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKF 300
           ENRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKF
Sbjct: 241 ENRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKF 300

Query: 301 KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 360
           KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY
Sbjct: 301 KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 360

Query: 361 DKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL 420
           DKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL
Sbjct: 361 DKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL 420

Query: 421 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 480
           YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG
Sbjct: 421 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 480

Query: 481 ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 540
           ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL
Sbjct: 481 ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 540

Query: 541 DTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAG 600
           DTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAG
Sbjct: 541 DTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAG 600

Query: 601 LSTN 604
           LSTN
Sbjct: 601 LSTN 604

BLAST of CsGy5G000410 vs. ExPASy TrEMBL
Match: A0A1S3AUZ6 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103482964 PE=3 SV=1)

HSP 1 Score: 1149 bits (2971), Expect = 0.0
Identity = 581/605 (96.03%), Postives = 589/605 (97.36%), Query Frame = 0

Query: 1   MNNNNSYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60
           MNNNN+Y +LHTSHSANTTPA SFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV
Sbjct: 1   MNNNNNYKQLHTSHSANTTPATSFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60

Query: 61  SAAILVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 120
           SAAILVRS + SSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI
Sbjct: 61  SAAILVRSRIASSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 120

Query: 121 SLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNH 180
           SLNLTLQHLTKALYSTSQIP+LQISKDPLAHSAYEDCMELLNDAIDAFSLSLFS  ASNH
Sbjct: 121 SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTGASNH 180

Query: 181 DIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240
           DIMTWLSAALTYHDTCT GFQDV DLGVK+EVEAKLSDLSEMISNSLAIFSGFGGGDLPV
Sbjct: 181 DIMTWLSAALTYHDTCTDGFQDVTDLGVKNEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240

Query: 241 ENRKRRRLMESST-TSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGK 300
           ENRKRRRLMES T +SWAAENGGDHEGFP WLSGKDRRLLAAP+S IQADIVVAKDGSGK
Sbjct: 241 ENRKRRRLMESGTPSSWAAENGGDHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDGSGK 300

Query: 301 FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV 360
           FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEE LKVGRKKTNLMFVGDGKGITVISGGKSV
Sbjct: 301 FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEEILKVGRKKTNLMFVGDGKGITVISGGKSV 360

Query: 361 YDKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDT 420
           YDKVTTFRTATFAGSGT IILRDM+FENTAGPSKHQAVALRLSADHAVVY CNIIGYQDT
Sbjct: 361 YDKVTTFRTATFAGSGTGIILRDMSFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDT 420

Query: 421 LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNT 480
           LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQK TITAQDRKDPNQNT
Sbjct: 421 LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKXTITAQDRKDPNQNT 480

Query: 481 GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFA 540
           GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFA
Sbjct: 481 GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFA 540

Query: 541 LDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSA 600
           LDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSA
Sbjct: 541 LDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSA 600

Query: 601 GLSTN 604
           GLSTN
Sbjct: 601 GLSTN 605

BLAST of CsGy5G000410 vs. ExPASy TrEMBL
Match: A0A6J1E2V1 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111430323 PE=3 SV=1)

HSP 1 Score: 1014 bits (2623), Expect = 0.0
Identity = 515/604 (85.26%), Postives = 553/604 (91.56%), Query Frame = 0

Query: 4   NNSYNKLHTSHSANTTPAASFRLSGN--DPPPPSSSLRRRSRILIISFMSVLLIAASVVS 63
           NN+YN LH+SHS +T PA+SFR+S +  DP PP S    R RIL+ISF S+LLI AS VS
Sbjct: 2   NNNYNMLHSSHSGDT-PASSFRVSNDHSDPLPPPSPSSSRRRILLISFFSILLITASAVS 61

Query: 64  AAILVRSHMTSS-QPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 123
           A +LV S + +S + HFPHNKP+QAISRACS TLYPSLCLSSLLS PG+ SADVHDLVHI
Sbjct: 62  AVVLVHSRIAASTESHFPHNKPTQAISRACSLTLYPSLCLSSLLSAPGSLSADVHDLVHI 121

Query: 124 SLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNH 183
           SLNLTLQHLTKALYSTSQIP+ QIS DPLA SAYEDCMELLNDA++AFSLSLFS  A+NH
Sbjct: 122 SLNLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTTATNH 181

Query: 184 DIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 243
           D+MTWLSAALTYHDTCT GF DVAD  +KD++ AKLSDLSEMISNSLAIFSGFGG DLPV
Sbjct: 182 DVMTWLSAALTYHDTCTDGFSDVAD-PIKDQITAKLSDLSEMISNSLAIFSGFGGDDLPV 241

Query: 244 ENRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKF 303
           ENR+RRRLMES     A EN GD + FP W+SGKDRRLLAAP+S+IQADIVVAKDGSGKF
Sbjct: 242 ENRRRRRLMESDEQWLAEENNGDGD-FPRWVSGKDRRLLAAPISSIQADIVVAKDGSGKF 301

Query: 304 KTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 363
           KTVAEAI AAPSSSGRRI+IYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY
Sbjct: 302 KTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVY 361

Query: 364 DKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTL 423
           DKVTTFRTATFAGSGT +ILRDMTFENTAGP+KHQAVALRLSADHAVV+  N+IGYQDTL
Sbjct: 362 DKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHAVVFRSNVIGYQDTL 421

Query: 424 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTG 483
           YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPM+GQKNTITAQDRKDPNQNTG
Sbjct: 422 YVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMSGQKNTITAQDRKDPNQNTG 481

Query: 484 ISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 543
           ISIHACKI+ATGDL+ASKGSFPT+LGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL
Sbjct: 482 ISIHACKIIATGDLQASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFAL 541

Query: 544 DTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAG 603
           DTLYYGEYMNSGPGAAVGQRVKWPGYRVITS +EA+KFTVGQFIYGSSWLPSTGVSFSAG
Sbjct: 542 DTLYYGEYMNSGPGAAVGQRVKWPGYRVITSAIEATKFTVGQFIYGSSWLPSTGVSFSAG 601

BLAST of CsGy5G000410 vs. ExPASy TrEMBL
Match: A0A6J1IA52 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111471502 PE=3 SV=1)

HSP 1 Score: 1006 bits (2602), Expect = 0.0
Identity = 513/607 (84.51%), Postives = 551/607 (90.77%), Query Frame = 0

Query: 4   NNSYNKLHTSHSANTTPAASFRLSGND-----PPPPSSSLRRRSRILIISFMSVLLIAAS 63
           NN+YNKLH+S S +T P  SFR+S +      PPPPSSS RR   IL+ISF S+LLI AS
Sbjct: 2   NNNYNKLHSSRSGDT-PVTSFRVSNDHSNPPPPPPPSSSHRR---ILLISFFSILLITAS 61

Query: 64  VVSAAILVRSHMTSS-QPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDL 123
            VSA +LV S +T+S + HFPHNKP+QAISRACS TLYPSLC+SSLLS PG+ SADVHDL
Sbjct: 62  TVSAVVLVHSRITTSTESHFPHNKPTQAISRACSLTLYPSLCISSLLSAPGSLSADVHDL 121

Query: 124 VHISLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDA 183
           VHISLNLTLQHLTKALYSTSQIP+ QIS DPLA SAYEDCMELLNDA++AFSLSLFS  A
Sbjct: 122 VHISLNLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTTA 181

Query: 184 SNHDIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGD 243
           +NHD+MTWLSAALTYHDTCT GF DVAD  VKD++ AKLSDLSEMISNSLAIFSGFGGGD
Sbjct: 182 TNHDVMTWLSAALTYHDTCTDGFSDVAD-PVKDQITAKLSDLSEMISNSLAIFSGFGGGD 241

Query: 244 LPVENRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGS 303
           LPVENR+RRRLMES     A EN GD + FP W+SGKDRRLLAAP+S+IQADIVVAKDGS
Sbjct: 242 LPVENRRRRRLMESDEQWLAEENNGDGD-FPRWVSGKDRRLLAAPISSIQADIVVAKDGS 301

Query: 304 GKFKTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK 363
           GKFKTVAEAI AAPSSS RRI+IYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK
Sbjct: 302 GKFKTVAEAIAAAPSSSSRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK 361

Query: 364 SVYDKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQ 423
           SVYDKVTTFRTATFAGSGT +ILRDMTFENTAGP+KHQAVALRLSADH VV+  N+IGYQ
Sbjct: 362 SVYDKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHTVVFRSNVIGYQ 421

Query: 424 DTLYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQ 483
           DTLYVHSNRQFFRECDIYGTIDFIFGNA VVFQSCNIYARKPM+GQKNTITAQDRKDPNQ
Sbjct: 422 DTLYVHSNRQFFRECDIYGTIDFIFGNAAVVFQSCNIYARKPMSGQKNTITAQDRKDPNQ 481

Query: 484 NTGISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGS 543
           NTGISIHACKI+ATGDL+ASKGSFPT+LGRPWKLYSRVVYMVSS+GDHIHPRGWLEWQGS
Sbjct: 482 NTGISIHACKIIATGDLQASKGSFPTYLGRPWKLYSRVVYMVSSLGDHIHPRGWLEWQGS 541

Query: 544 FALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSF 603
           FALDTLYYGEYMNSGP AAVGQRVKWPGYRVITS +EA+KFTVGQFIYGSSWLPSTGVSF
Sbjct: 542 FALDTLYYGEYMNSGPSAAVGQRVKWPGYRVITSAIEATKFTVGQFIYGSSWLPSTGVSF 601

BLAST of CsGy5G000410 vs. ExPASy TrEMBL
Match: A0A6J1H2K1 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111459120 PE=3 SV=1)

HSP 1 Score: 978 bits (2528), Expect = 0.0
Identity = 505/602 (83.89%), Postives = 543/602 (90.20%), Query Frame = 0

Query: 5   NSYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVVSAAI 64
           N+YNKLH+S S +T   A+F++S    PP SS+ RRR+RIL +S +S+LLI AS VSAA+
Sbjct: 2   NNYNKLHSSASGDTP--ATFQVSHTPNPPSSSASRRRTRILFVSLISILLITASAVSAAV 61

Query: 65  LVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHISLNL 124
           L+ S  +    HFPHNKP+QAIS ACSHTLYPSLC SSLLSFPG+ SADVH LVHISLNL
Sbjct: 62  LLHSQSS----HFPHNKPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHRLVHISLNL 121

Query: 125 TLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNHDIMT 184
           TLQHLTKALY++SQIPV QIS +PLAHSAY+DC+ELLNDA+DAFSLSLFS  ASNHDIMT
Sbjct: 122 TLQHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHDIMT 181

Query: 185 WLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPVENRK 244
           WLSAALTYHDTC+ GF DV D  VKD++ A+LSDLSEMISNSLAIFSG+G G LPV+NR 
Sbjct: 182 WLSAALTYHDTCSDGFADVDD-PVKDQMAARLSDLSEMISNSLAIFSGYGEG-LPVQNR- 241

Query: 245 RRRLMESSTTSWAAENG-GDHEG-FPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKT 304
            RRLMES    WAAENG    EG FP W+SGKDRRLLAAP+S IQADIVVAKDGSGKFKT
Sbjct: 242 -RRLMESDR-EWAAENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKFKT 301

Query: 305 VAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDK 364
           VAEAIEAAPSSSGRRI+IYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV+DK
Sbjct: 302 VAEAIEAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFDK 361

Query: 365 VTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYV 424
           VTTFRTATFAGSGT IILRDMTFENTAGPSKHQAVALR+SADHAVVY CNIIGYQDTLYV
Sbjct: 362 VTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRVSADHAVVYRCNIIGYQDTLYV 421

Query: 425 HSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGIS 484
           HSNRQFFRECDIYGT+DFIFGNAVVVFQSCNIYARKPM  QKNTITAQ+RKDPNQNTGIS
Sbjct: 422 HSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQNRKDPNQNTGIS 481

Query: 485 IHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDT 544
           IHACKI+AT DLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDT
Sbjct: 482 IHACKILATTDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDT 541

Query: 545 LYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGLS 604
           LYYGEYMNSGPGAA GQRV WPGYRVITST+EA+KFTVGQFIYGSSWLPSTGVSFSAGLS
Sbjct: 542 LYYGEYMNSGPGAATGQRVNWPGYRVITSTIEATKFTVGQFIYGSSWLPSTGVSFSAGLS 592

BLAST of CsGy5G000410 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 703.4 bits (1814), Expect = 1.6e-202
Identity = 365/594 (61.45%), Postives = 438/594 (73.74%), Query Frame = 0

Query: 20  PAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVVSAAILVRSHMTSSQPHFPH 79
           P+     +  DP      L+++++  +I F   +L+   VV   I        S    P 
Sbjct: 8   PSGPSNPNQKDPATSLPELQKKTKTKLILFTLAVLV-VGVVCFGIFAGIRAVDSGKTEPK 67

Query: 80  --NKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHISLNLTLQHLTKALYSTS 139
              KP+QAISR CS +LYP+LC+ +LL FPG+ +AD ++L+HIS N TLQ  +KALY++S
Sbjct: 68  LTRKPTQAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSS 127

Query: 140 QIPVLQISKDPLAHSAYEDCMELLNDAIDAF-----SLSLFSKDASNHDIMTWLSAALTY 199
            I   Q+   P   SAY+ C+ELL+D++DA      S+ + S D S+ D+MTWLS+A+T 
Sbjct: 128 TITYTQM--PPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHSDVMTWLSSAMTN 187

Query: 200 HDTCTAGFQDVADLG--VKDEVEAKLSDLSEMISNSLAIFSG--FGGGDLPVENRKRRRL 259
           HDTCT GF ++   G  VKD+V   + DLSEM+SN LAIF+G       +PV N   R+L
Sbjct: 188 HDTCTDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVN--NRKL 247

Query: 260 MESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKTVAEAIE 319
           + +  T          E  P WL  +DR LL  P S IQADI V+KDGSG FKT+AEAI+
Sbjct: 248 LGTEET----------EELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIK 307

Query: 320 AAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRT 379
            AP  S RR +IY+KAG+YEEENLKVGRKKTNLMF+GDGKG TVI+GGKS+ D +TTF T
Sbjct: 308 KAPEHSSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHT 367

Query: 380 ATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQF 439
           ATFA +G   I+RDMTFEN AGP+KHQAVALR+  DHAVVY CNIIGYQD LYVHSNRQF
Sbjct: 368 ATFAATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQF 427

Query: 440 FRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKI 499
           FREC+IYGT+DFIFGNA V+ QSCNIYARKPMA QK TITAQ+RKDPNQNTGISIHACK+
Sbjct: 428 FRECEIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKL 487

Query: 500 VATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEY 559
           +AT DLEASKGS+PT+LGRPWKLYSRVVYM+S MGDHI PRGWLEW G FALD+LYYGEY
Sbjct: 488 LATPDLEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEY 547

Query: 560 MNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGLS 603
           MN G G+ +GQRVKWPGY VITSTVEASKFTV QFI GSSWLPSTGVSF +GLS
Sbjct: 548 MNKGLGSGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGLS 586

BLAST of CsGy5G000410 vs. TAIR 10
Match: AT3G49220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 684.5 bits (1765), Expect = 7.8e-197
Identity = 347/599 (57.93%), Postives = 439/599 (73.29%), Query Frame = 0

Query: 13  SHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISF-MSVLLIAASVVSAAILVRSHMT 72
           S + +TT A           P +++ R + ++++ S  +++ LI A+ + A +  R  + 
Sbjct: 14  SVTTSTTTAPILNQVSTSEQPENNNRRSKKKLVVSSIVLAISLILAAAIFAGVRSRLKLN 73

Query: 73  SSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSA-DVHDLVHISLNLTLQHLT 132
            S P     KPSQAIS+AC  T +P LC+ SL+ FPG+ +A    DL+H+++N+TL H +
Sbjct: 74  QSVPGLA-RKPSQAISKACELTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHFS 133

Query: 133 KALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFS---LSLFSKDASNHDIMTWLS 192
            ALYS++ +    +   P A SAY+ C+ELL+D++DA S    S+ S  A   D+ TWLS
Sbjct: 134 HALYSSASLSF--VDMPPRARSAYDSCVELLDDSVDALSRALSSVVSSSAKPQDVTTWLS 193

Query: 193 AALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGD----LPVENR 252
           AALT HDTCT GF  V D GVKD + A L +LSE++SN LAIFS    GD    +P++NR
Sbjct: 194 AALTNHDTCTEGFDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNR 253

Query: 253 KRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKTV 312
           +   + E              E FP W+  K+R +L  P+S IQADI+V+KDG+G  KT+
Sbjct: 254 RLLGVEER------------EEKFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKTI 313

Query: 313 AEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKV 372
           +EAI+ AP +S RRIIIY+KAG+YEE NLKVGRKK NLMFVGDGKG TVISGGKS++D +
Sbjct: 314 SEAIKKAPQNSTRRIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDNI 373

Query: 373 TTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVH 432
           TTF TA+FA +G   I RD+TFEN AGP+KHQAVALR+ ADHAV+Y CNIIGYQDTLYVH
Sbjct: 374 TTFHTASFAATGAGFIARDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYVH 433

Query: 433 SNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISI 492
           SNRQFFRECDIYGT+DFIFGNA VV Q+C+IYARKPM  QKNTITAQ+RKDPNQNTGISI
Sbjct: 434 SNRQFFRECDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGISI 493

Query: 493 HACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTL 552
           HA +++A  DL+A+ GS  T+LGRPWKL+SR VYM+S +G H+H RGWLEW  +FALDTL
Sbjct: 494 HASRVLAASDLQATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDTL 553

Query: 553 YYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGLS 603
           YYGEY+NSGPG+ +GQRV WPGYRVI ST EA++FTV +FIYGSSWLPSTGVSF AGLS
Sbjct: 554 YYGEYLNSGPGSGLGQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGLS 597

BLAST of CsGy5G000410 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 469.5 bits (1207), Expect = 3.9e-132
Identity = 260/589 (44.14%), Postives = 364/589 (61.80%), Query Frame = 0

Query: 37  SLRRRSRILIISFMSVLLIAASV--VSAAILVRSHMTSSQPHFPHNKPSQAISR-ACSHT 96
           + ++  +++++S    LL  A+V  +SA     +   +  P       S A+ R +CS T
Sbjct: 13  NFKKNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSP------SSHAVLRSSCSST 72

Query: 97  LYPSLCLSSLLSFPGAQSADVHDLVHISLNLTLQHLTKALYSTSQIPVLQISKDPLAHSA 156
            YP LC+S++++  G +     D++  S+NLT+  +    ++  ++   +    P   +A
Sbjct: 73  RYPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTA 132

Query: 157 YEDCMELLNDAIDAF-----SLSLF--SKDASNH--DIMTWLSAALTYHDTCTAGF-QDV 216
             DC+E +++ +D        L L+   K    H  D+ T +S+A+T  +TC  GF  D 
Sbjct: 133 LHDCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDD 192

Query: 217 ADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDL-------PVENRKRRRLMESSTTSW 276
           AD  V+  +      +  M SN+LA+       D+        + +  R+   E+  T+ 
Sbjct: 193 ADKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTV 252

Query: 277 AAENGG----DHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKTVAEAIEAAPS 336
           A +  G    D EG+P WLS  DRRLL    S ++AD  VA DGSG FKTVA A+ AAP 
Sbjct: 253 AVDIAGAGELDSEGWPTWLSAGDRRLLQG--SGVKADATVAADGSGTFKTVAAAVAAAPE 312

Query: 337 SSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATFA 396
           +S +R +I+IKAG Y  EN++V +KK N+MF+GDG+  T+I+G ++V D  TTF +AT A
Sbjct: 313 NSNKRYVIHIKAGVY-RENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVA 372

Query: 397 GSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFREC 456
             G   + RD+TF+NTAGPSKHQAVALR+ +D +  Y+C+++ YQDTLYVHSNRQFF +C
Sbjct: 373 AVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKC 432

Query: 457 DIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVATG 516
            I GT+DFIFGNA VV Q C+I+AR+P +GQKN +TAQ R DPNQNTGI I  C+I AT 
Sbjct: 433 LIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATS 492

Query: 517 DLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSG 576
           DL++ KGSFPT+LGRPWK YS+ V M S++ D I P GW EW G+FAL+TL Y EY N+G
Sbjct: 493 DLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTG 552

Query: 577 PGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 602
            GA    RVKW G++VIT+  EA K+T GQFI G  WL STG  FS GL
Sbjct: 553 AGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

BLAST of CsGy5G000410 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 468.8 bits (1205), Expect = 6.7e-132
Identity = 263/580 (45.34%), Postives = 350/580 (60.34%), Query Frame = 0

Query: 36  SSLRRRSRILIISFMSVLLIAASVVSAAILVRSHMTSSQPHFPHNKPSQAISRA-CSHTL 95
           S  +   ++++ S    LL+ AS+V  A    ++   +Q     +  S AI ++ CS TL
Sbjct: 12  SDFKNNKKLILSSAAIALLLLASIVGIA-ATTTNQNKNQKITTLSSTSHAILKSVCSSTL 71

Query: 96  YPSLCLSSLLSFPGAQSADVHDLVHISLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAY 155
           YP LC S++ +  G +     +++  SLNLT + +    ++  ++   +    P   +A 
Sbjct: 72  YPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTAL 131

Query: 156 EDCMELLNDAIDAFSLSL-------FSKDASNH--DIMTWLSAALTYHDTCTAGFQ-DVA 215
            DC+E +++ +D   +++         K    H  D+ T +S+A+T   TC  GF  D A
Sbjct: 132 HDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDA 191

Query: 216 DLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLP-VENRKRRRLMESSTTSWAAENGG- 275
           D  V+  +      +  M SN+LA+       D+   E R +     ++      E  G 
Sbjct: 192 DRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKEVTGD 251

Query: 276 -DHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKTVAEAIEAAPSSSGRRIIIY 335
            D +G+P WLS  DRRLL    STI+AD  VA DGSG F TVA A+ AAP  S +R +I+
Sbjct: 252 LDSDGWPKWLSVGDRRLLQG--STIKADATVADDGSGDFTTVAAAVAAAPEKSNKRFVIH 311

Query: 336 IKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATFAGSGTNIILR 395
           IKAG Y  EN++V +KKTN+MF+GDG+G T+I+G ++V D  TTF +AT A  G   + R
Sbjct: 312 IKAGVY-RENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVGERFLAR 371

Query: 396 DMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFRECDIYGTIDFI 455
           D+TF+NTAGPSKHQAVALR+ +D +  Y C++  YQDTLYVHSNRQFF +C I GT+DFI
Sbjct: 372 DITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCHITGTVDFI 431

Query: 456 FGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVATGDLEASKGSF 515
           FGNA  V Q C+I AR+P +GQKN +TAQ R DPNQNTGI I  C+I  T DL A KG+F
Sbjct: 432 FGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLLAVKGTF 491

Query: 516 PTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAAVGQRV 575
           PT+LGRPWK YSR V M S + D I P GW EW GSFALDTL Y EY+N G GA    RV
Sbjct: 492 PTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRGGGAGTANRV 551

Query: 576 KWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 602
           KW GY+VITS  EA  FT GQFI G  WL STG  FS  L
Sbjct: 552 KWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

BLAST of CsGy5G000410 vs. TAIR 10
Match: AT3G43270.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 420.6 bits (1080), Expect = 2.1e-117
Identity = 224/460 (48.70%), Postives = 295/460 (64.13%), Query Frame = 0

Query: 152 SAYEDCMELLNDAIDAFSLSLFSKDASN----------HDIMTWLSAALTYHDTCTAGFQ 211
           +A  DC++LL+ A +  S  + +  + N           D+ TW+SAAL+  DTC  GF+
Sbjct: 79  NAIVDCVDLLDSAAEELSWIISASQSPNGKDNSTGDVGSDLRTWISAALSNQDTCLDGFE 138

Query: 212 DVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPVENRKRRRLMESSTTSWAAENG 271
               + +K  V   LS +   + N L +         P    K + +   + T   A +G
Sbjct: 139 GTNGI-IKKIVAGGLSKVGTTVRNLLTMVHS------PPSKPKPKPIKAQTMTK--AHSG 198

Query: 272 GDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGKFKTVAEAIEAAPSSSGRRIIIY 331
                FP+W+   DR+LL     T+ AD VVA DG+G F T+++A+ AAP  S +R +I+
Sbjct: 199 --FSKFPSWVKPGDRKLLQTDNITV-ADAVVAADGTGNFTTISDAVLAAPDYSTKRYVIH 258

Query: 332 IKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATFAGSGTNIILR 391
           +K G Y  EN+++ +KK N+M VGDG   TVI+G +S  D  TTFR+ATFA SG   I R
Sbjct: 259 VKRGVY-VENVEIKKKKWNIMMVGDGIDATVITGNRSFIDGWTTFRSATFAVSGRGFIAR 318

Query: 392 DMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFRECDIYGTIDFI 451
           D+TF+NTAGP KHQAVA+R   D  V Y C + GYQDTLY HS RQFFREC I GT+DFI
Sbjct: 319 DITFQNTAGPEKHQAVAIRSDTDLGVFYRCAMRGYQDTLYAHSMRQFFRECIITGTVDFI 378

Query: 452 FGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVATGDLEASKGSF 511
           FG+A  VFQSC I A++ +  QKN+ITAQ RKDPN+ TG +I    I A  DL  +  + 
Sbjct: 379 FGDATAVFQSCQIKAKQGLPNQKNSITAQGRKDPNEPTGFTIQFSNIAADTDLLLNLNTT 438

Query: 512 PTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAAVGQRV 571
            T+LGRPWKLYSR V+M + M D I+P GWLEW G+FALDTLYYGEYMNSGPGA++ +RV
Sbjct: 439 ATYLGRPWKLYSRTVFMQNYMSDAINPVGWLEWNGNFALDTLYYGEYMNSGPGASLDRRV 498

Query: 572 KWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 602
           KWPGY V+ ++ EA+ FTV Q I G+ WLPSTG++F AGL
Sbjct: 499 KWPGYHVLNTSAEANNFTVSQLIQGNLWLPSTGITFIAGL 525

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FK052.3e-20161.45Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3... [more]
Q9M3B01.1e-19557.93Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana OX=3... [more]
O490065.5e-13144.14Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME... [more]
Q425349.5e-13145.34Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2[more]
P839481.0e-12944.67Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_011654619.10.0100.00probable pectinesterase/pectinesterase inhibitor 61 [Cucumis sativus] >KGN49865.... [more]
XP_008437596.10.096.03PREDICTED: LOW QUALITY PROTEIN: probable pectinesterase/pectinesterase inhibitor... [more]
XP_038874841.10.089.90probable pectinesterase/pectinesterase inhibitor 61 [Benincasa hispida][more]
XP_022922302.10.085.26probable pectinesterase/pectinesterase inhibitor 61 [Cucurbita moschata][more]
KAG6579321.10.085.26putative pectinesterase/pectinesterase inhibitor 61, partial [Cucurbita argyrosp... [more]
Match NameE-valueIdentityDescription
A0A0A0KJG10.0100.00Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G139430 PE=3 SV=1[more]
A0A1S3AUZ60.096.03Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103482964 PE=3 SV=1[more]
A0A6J1E2V10.085.26Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111430323 PE=3 SV=1[more]
A0A6J1IA520.084.51Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111471502 PE=3 SV=1[more]
A0A6J1H2K10.083.89Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111459120 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G53370.11.6e-20261.45pectin methylesterase PCR fragment F [more]
AT3G49220.17.8e-19757.93Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G14310.13.9e-13244.14pectin methylesterase 3 [more]
AT1G53830.16.7e-13245.34pectin methylesterase 2 [more]
AT3G43270.12.1e-11748.70Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 81..229
e-value: 4.3E-41
score: 152.5
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 54..231
e-value: 2.3E-33
score: 113.0
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 85..229
e-value: 4.4E-31
score: 108.3
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 83..233
e-value: 3.2E-36
score: 126.5
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 84..231
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 283..601
e-value: 2.2E-121
score: 407.2
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 289..586
e-value: 5.4E-144
score: 478.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..38
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..36
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 37..601
NoneNo IPR availablePANTHERPTHR31707:SF184PECTINESTERASEcoord: 37..601
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 89..235
e-value: 1.74937E-36
score: 131.792
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 434..443
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 284..600

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy5G000410.2CsGy5G000410.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity