CsGy4G022500 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy4G022500
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionRING-type domain-containing protein
LocationGy14Chr4: 28958367 .. 28962535 (+)
RNA-Seq ExpressionCsGy4G022500
SyntenyCsGy4G022500
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATTTGCAGCAGTGGCGTATTGGTGTTGACAAGCTATCTACTTGATCTCCACCTTCTTTCTTCCTTCATTTCTTCCTTTCTTTCTTCCTTCCTTCCTCCGTTTTCTCTCTCGGGCAATTCATCGCTCCATGGCTCTTCCTCAGAATCACTACCAACAACACTATCAATCTCAACACCAACAGCCACACCAATCTATATCTTTTCGGTAATTTTTCTTCTTCACAATGTAAAACCACGAAAAGTTTTTCTTTTAAAACCCTAGCGATTCCCCTCTTTTCAATCTGTTTTCCATTCTTCTAATTCCGCTTTCTCCTATCTTATTGCAGCAACTTGTACACTCCCGATGGTCATGTTTCTCAACCGATCTCCTATTTCAACTCCTTTACTTTGCAGGATCATTCCCAGCATCCTCCCTACGTTCCTCCATGTAAATTTTCTTCCTTTCCTTTCTTTTATTTTCTCTCTTTCTTCATTTCTGTCAGCTTGTTCTTCTCCAGAAATTTTGGGTTTTTGCTGGTTTGTTGGTATAACATTTAAAGAAAACGGTATTTCTTCTCAAAACGGTTGGTTATTTTGTTCGATTGTTTAAAGACTTTGGGCTTGATTTTAGTTGTCTGGGCTAGTTCCATCTGCAGTTTTATTTGAGACTAACATTTTTGTTTCCCGCTTGTATTTTTCTGTTATATTAGTTAATGTAGTCGGGTTTGCACCAGGTACAGCCCCTGGTCAGGATGCTAGTGATGGAGTAGCAGATTTACACTGGATGTATGGCATTGAACCCAAGAAGAAGAGATTGAAAGAGCAAGATCTTTTTGAGAATAATTCCCAAATATCATCCATTGATTTCTTGCAGCAACGTCCAGTTTCTACAGGATTGGGTTTATCTCTTGACAACACTCGTATGGCTTCGACGGGAGACTCTCCTTTGGTTTCTCTTGTAGGTGATGACATCGATCGTGAATTGCAGCAACAGGACGAAGAAATTGAGAAGTTCCTTAAAGTTCAGGTTTGGATTACCATACTTTCATAACATTGGATATATAGAGTATTTACTAAACTGGAAAATTTCAAACCAGGGAGAAAAGTCAGGGTACTCAGACTAACTAATTAAGTACTACTTTTATTACGTTCTTTGGATAAAAAATGAAATCCTTCCTGAGCCCAAGCATACCTCTTTAAGAAATAGAGGTTCGGACGAGGTATAGGACTTTCTTATGTAGCTGGGTTGGTATATGAGTATAGCATTAAAAAGAAGTATAAGACTTTCCAGTGAGGTGGTACATTATTCTCTTATTCTCATGCTAGTTACTTCAACCCGATGGTCTTAATCTACCTCTATTCCAAGTATACCCTTCCGAGTTCTGATGCATACTAACTTAATTTTCCCTCTACTTAAACGGAGGTTTATTGATACTATCATGTAGTGTCTTTAATTATTTTCTGCAGAACATTAAAATTAGGGGTTGTTGGATCTTGAATGTATTTCTTGGTAATCTTCATGTCCTTCTGATGAAAGAAGACAAGATGCAATATGCCTACCATGATGTTTGTTTCTATGTGTTGGCATGCTTTACTGGTTATTTTCTCCGTTTAGACTGTTTCAGAGTGTTGGTTCTCCTTGATTTTGAGCTCGGCTTTCTATTATTTTAGATGCTTTTGAATCTTCTTGTCATATTTCTTTAATCTTGAATGTCTAAGTTGACATACATCAACTAGTCACATGAGATAACTTCTTAATCCTTTCGATTAAGGTAGGTAATGTTTTAAATATATCGTTACCTTAGTTTTAACCAACTAATTCATAGACATTGGGGTTTATAGCTTGCCCTTCCCATCATTGTACTAATCCTTCTTTTGTAGACTATCAGGGTTATACTTATTTTTGAATTTGGATTTAAATTTTCAAGGAACTTACAATATTATGATCTGGAAGCATATTCCAGAGTTCTCCTTCTAGGCCCTTTGATATTGATCCTTCAGTGGCGTTTTTTTTGTTAGTTTAATAGTTTCTTTCTGATTAGTTGTGGCCTTGCTCGTTTTGTAATTTCCACAGTGGTTTTCTTTGAGATGTTGTATGACATCAAGAATGACTTCATGGCTATTTCAATAGTGTGTGGATTGTGGGTCCTGAATTTAACCATATTTTTTAAGGTTAAATTTTAACCATATACCTTCATCTTCTAATCCTCATTCTATTTATAGGGTGATCGGCTACGGCATTCCATATTAGAGAAGATCCAAGCAAATCAACTCCAAACTCTCTCGATTGTAGAGGAAAAAATTATTAAAAAGCTACGTGAAAAAGAAGCTGAGGTGGAGTGTATCAACAAGAAAAACATTGAGCTAGAACAGCGAATGGAGCAGCTTTCAGTGGAAGCTGGTGCTTGGCAGCAGCGCGCTAGGTATAACGAGAACATGATCACAGCCCTGAAGTTCAATCTTCAGCAGGTGTATGCTCAAAGCAGGGATAGCAAGGAGGGATGTGGGGACAGTGAGGTTGAAGACACTGCATCGTGCTGCAATGGTCGGACGCTTGATTTTCAACTGCTTTGTAGCAACAGCAATGATGTGAAGGAGTTGATGTACTGTAAGGCTTGCAGGGTTAATGAAGTGTGCATGCTTTTGTTCCCTTGTAAGCATCTCTGCCTGTGCAAGGATTGCGAAAGTAGGCTTACATTTTGCCCTGTGTGTCAATCGTCGAAATTTGTAGGGATGGAAGTTTATATGTAAAAGGATAATTTGTGTACGACCCCCAAATATATGCTAAATTCATATACATTTCTCTTGTGATAATTATGTACTTGTTTCCCCC

mRNA sequence

AAATTTGCAGCAGTGGCGTATTGGTGTTGACAAGCTATCTACTTGATCTCCACCTTCTTTCTTCCTTCATTTCTTCCTTTCTTTCTTCCTTCCTTCCTCCGTTTTCTCTCTCGGGCAATTCATCGCTCCATGGCTCTTCCTCAGAATCACTACCAACAACACTATCAATCTCAACACCAACAGCCACACCAATCTATATCTTTTCGCAACTTGTACACTCCCGATGGTCATGTTTCTCAACCGATCTCCTATTTCAACTCCTTTACTTTGCAGGATCATTCCCAGCATCCTCCCTACGTTCCTCCATGTACAGCCCCTGGTCAGGATGCTAGTGATGGAGTAGCAGATTTACACTGGATGTATGGCATTGAACCCAAGAAGAAGAGATTGAAAGAGCAAGATCTTTTTGAGAATAATTCCCAAATATCATCCATTGATTTCTTGCAGCAACGTCCAGTTTCTACAGGATTGGGTTTATCTCTTGACAACACTCGTATGGCTTCGACGGGAGACTCTCCTTTGGTTTCTCTTGTAGGTGATGACATCGATCGTGAATTGCAGCAACAGGACGAAGAAATTGAGAAGTTCCTTAAAGTTCAGGGTGATCGGCTACGGCATTCCATATTAGAGAAGATCCAAGCAAATCAACTCCAAACTCTCTCGATTGTAGAGGAAAAAATTATTAAAAAGCTACGTGAAAAAGAAGCTGAGGTGGAGTGTATCAACAAGAAAAACATTGAGCTAGAACAGCGAATGGAGCAGCTTTCAGTGGAAGCTGGTGCTTGGCAGCAGCGCGCTAGGTATAACGAGAACATGATCACAGCCCTGAAGTTCAATCTTCAGCAGGTGTATGCTCAAAGCAGGGATAGCAAGGAGGGATGTGGGGACAGTGAGGTTGAAGACACTGCATCGTGCTGCAATGGTCGGACGCTTGATTTTCAACTGCTTTGTAGCAACAGCAATGATGTGAAGGAGTTGATGTACTGTAAGGCTTGCAGGGTTAATGAAGTGTGCATGCTTTTGTTCCCTTGTAAGCATCTCTGCCTGTGCAAGGATTGCGAAAGTAGGCTTACATTTTGCCCTGTGTGTCAATCGTCGAAATTTGTAGGGATGGAAGTTTATATGTAAAAGGATAATTTGTGTACGACCCCCAAATATATGCTAAATTCATATACATTTCTCTTGTGATAATTATGTACTTGTTTCCCCC

Coding sequence (CDS)

ATGGCTCTTCCTCAGAATCACTACCAACAACACTATCAATCTCAACACCAACAGCCACACCAATCTATATCTTTTCGCAACTTGTACACTCCCGATGGTCATGTTTCTCAACCGATCTCCTATTTCAACTCCTTTACTTTGCAGGATCATTCCCAGCATCCTCCCTACGTTCCTCCATGTACAGCCCCTGGTCAGGATGCTAGTGATGGAGTAGCAGATTTACACTGGATGTATGGCATTGAACCCAAGAAGAAGAGATTGAAAGAGCAAGATCTTTTTGAGAATAATTCCCAAATATCATCCATTGATTTCTTGCAGCAACGTCCAGTTTCTACAGGATTGGGTTTATCTCTTGACAACACTCGTATGGCTTCGACGGGAGACTCTCCTTTGGTTTCTCTTGTAGGTGATGACATCGATCGTGAATTGCAGCAACAGGACGAAGAAATTGAGAAGTTCCTTAAAGTTCAGGGTGATCGGCTACGGCATTCCATATTAGAGAAGATCCAAGCAAATCAACTCCAAACTCTCTCGATTGTAGAGGAAAAAATTATTAAAAAGCTACGTGAAAAAGAAGCTGAGGTGGAGTGTATCAACAAGAAAAACATTGAGCTAGAACAGCGAATGGAGCAGCTTTCAGTGGAAGCTGGTGCTTGGCAGCAGCGCGCTAGGTATAACGAGAACATGATCACAGCCCTGAAGTTCAATCTTCAGCAGGTGTATGCTCAAAGCAGGGATAGCAAGGAGGGATGTGGGGACAGTGAGGTTGAAGACACTGCATCGTGCTGCAATGGTCGGACGCTTGATTTTCAACTGCTTTGTAGCAACAGCAATGATGTGAAGGAGTTGATGTACTGTAAGGCTTGCAGGGTTAATGAAGTGTGCATGCTTTTGTTCCCTTGTAAGCATCTCTGCCTGTGCAAGGATTGCGAAAGTAGGCTTACATTTTGCCCTGTGTGTCAATCGTCGAAATTTGTAGGGATGGAAGTTTATATGTAA

Protein sequence

MALPQNHYQQHYQSQHQQPHQSISFRNLYTPDGHVSQPISYFNSFTLQDHSQHPPYVPPCTAPGQDASDGVADLHWMYGIEPKKKRLKEQDLFENNSQISSIDFLQQRPVSTGLGLSLDNTRMASTGDSPLVSLVGDDIDRELQQQDEEIEKFLKVQGDRLRHSILEKIQANQLQTLSIVEEKIIKKLREKEAEVECINKKNIELEQRMEQLSVEAGAWQQRARYNENMITALKFNLQQVYAQSRDSKEGCGDSEVEDTASCCNGRTLDFQLLCSNSNDVKELMYCKACRVNEVCMLLFPCKHLCLCKDCESRLTFCPVCQSSKFVGMEVYM*
Homology
BLAST of CsGy4G022500 vs. ExPASy Swiss-Prot
Match: F4IDI6 (Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=3702 GN=BRG2 PE=1 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 3.8e-25
Identity = 88/285 (30.88%), Postives = 139/285 (48.77%), Query Frame = 0

Query: 68  SDGVADLHWMYGIEPKKKRLKEQDLFENNSQISSIDFLQQRPVSTGLGLSLDNTRMASTG 127
           +DG  +L   +   P +KR ++      +S+ +    L Q P S+    S  N    +T 
Sbjct: 83  ADGATNLDCEFFPLPTRKRSRD------SSRSNYHHLLLQNPRSS----SCVNAATTTT- 142

Query: 128 DSPLVSLVGDDID--RELQQQDEEIEKFLKV---QGDRLRHSILEKIQANQLQTLSIVEE 187
            + L S +G DID    + QQ  EI++F+ +   Q +R+++ I EK +      +  +E+
Sbjct: 143 TTTLFSFLGQDIDISSHMNQQQHEIDRFVSLHLYQMERVKYEIEEKRKRQARTIMEAIEQ 202

Query: 188 KIIKKLREKEAEVECINKKNIELEQRMEQLSVEAGAWQQRARYNENMITALKFNLQQVYA 247
            ++K+LR KE E E I K N  LE+R++ LS+E   W+  A+ NE     L+ NL+ V A
Sbjct: 203 GLVKRLRVKEEERERIGKVNHALEERVKSLSIENQIWRDLAQTNEATANHLRTNLEHVLA 262

Query: 248 QSRDSKEGCG----DSEVEDTASCCNG-----------RTLDFQLLCSNSNDVKELMYCK 307
           Q +D   G G     +E +D  SCC             R +  +    +  + +    C+
Sbjct: 263 QVKDVSRGAGLEKNMNEEDDAESCCGSSCGGGGEETVRRRVGLEREAQDKAERRRRRMCR 322

Query: 308 ACRVNEVCMLLFPCKHLCLCKDCESRLTFCPVCQSSKFVGMEVYM 333
            C   E C+LL PC+HLCLC  C S +  CP+C S K   + V M
Sbjct: 323 NCGEEESCVLLLPCRHLCLCGVCGSSVHTCPICTSPKNASVHVNM 356

BLAST of CsGy4G022500 vs. ExPASy Swiss-Prot
Match: Q9LDD1 (Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=3702 GN=BRG3 PE=1 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 1.2e-21
Identity = 67/211 (31.75%), Postives = 109/211 (51.66%), Query Frame = 0

Query: 130 PLVSLVGDDIDRELQQQDEEIEKFLKVQGDRLRHSILEKIQANQLQTLSIVEEKIIKKLR 189
           PL+ L G D+   +QQ   +I++ +    +R+R  I EK +    + +  VE+ ++K LR
Sbjct: 122 PLMFL-GQDLSSNVQQHHFDIDRLISNHVERMRMEIEEKRKTQGRRIVEAVEQGLMKTLR 181

Query: 190 EKEAEVECINKKNIELEQRMEQLSVEAGAWQQRARYNENMITALKFNLQQVYAQSRDSKE 249
            K+ E+  I K N+ LE++++ L VE   W+  A+ NE  + AL+ NLQQV A    ++ 
Sbjct: 182 AKDDEINHIGKLNLFLEEKVKSLCVENQIWRDVAQSNEATVNALRSNLQQVLAAVERNRW 241

Query: 250 GCGDSEVEDTASCCNGR-----TLDFQLLCSNSNDVKE-----LMYCKACRVNEVCMLLF 309
               +  +D  SCC          +   L   + D K+     +  C++C   E  +LL 
Sbjct: 242 EEPPTVADDAQSCCGSNDEGDSEEERWKLAGEAQDTKKMCRVGMSMCRSCGKGEASVLLL 301

Query: 310 PCKHLCLCKDCESRLTFCPVCQSSKFVGMEV 331
           PC+H+CLC  C S L  CP+C+S K   + V
Sbjct: 302 PCRHMCLCSVCGSSLNTCPICKSPKTASLHV 331

BLAST of CsGy4G022500 vs. ExPASy Swiss-Prot
Match: Q9FHE4 (BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG1 PE=1 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 6.2e-15
Identity = 63/216 (29.17%), Postives = 99/216 (45.83%), Query Frame = 0

Query: 133 SLVGDDIDRELQQQDEEIEKFLKVQGDRLRHSILEKIQANQLQTLSIVEEKIIKKLREKE 192
           SL+  ++  ++QQQ+ EI++F+  Q + LR  +  + +       S V+  I+KKL+ K+
Sbjct: 96  SLIDAELVSQIQQQNSEIDRFVAQQTETLRIELEARQRTQTRMLASAVQNAILKKLKAKD 155

Query: 193 AEVECINKKNIELEQRMEQLSVEAGAWQQRARYNENMITALKFNLQQVYAQ--------- 252
            E+  + K N  L++R++ L VE   W+  A+ NE     L+ NL+QV AQ         
Sbjct: 156 EEIIRMGKLNWVLQERVKNLYVENQIWRDLAQTNEATANNLRSNLEQVLAQVDDLDAFRR 215

Query: 253 -----SRDSKEGCGDSEVEDTASCCNGRTLDFQLLCSNSNDVKELMYCKACRVNEVCMLL 312
                + D++  CG  +  D  +  NG                    CK C      +L+
Sbjct: 216 PLVEEADDAESSCGSCDGGDVTAVVNGG-------------------CKRCGELTASVLV 275

Query: 313 FPCKHLCLCKDCESR--LTFCPVCQSSKFVGMEVYM 333
            PC+HLCLC  C S   L  CPVC       + V M
Sbjct: 276 LPCRHLCLCTVCGSSALLRTCPVCDMVMTASVHVNM 292

BLAST of CsGy4G022500 vs. ExPASy Swiss-Prot
Match: O81851 (E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 1.1e-14
Identity = 69/213 (32.39%), Postives = 98/213 (46.01%), Query Frame = 0

Query: 128 DSPLVSLVGDDIDRELQ-QQDEEIEKFLKVQGDRLRHSILEKIQANQLQTLSIVEEKIIK 187
           DS   SL+  ++  ++Q QQ  EI++F+  Q ++LR  I  + Q       S V+  I K
Sbjct: 100 DSSSPSLINVELVSQIQNQQQSEIDRFVAQQTEKLRIEIEARQQTQTRMLASAVQNVIAK 159

Query: 188 KLREKEAEVECINKKNIELEQRMEQLSVEAGAWQQRARYNENMITALKFNLQQVYAQSRD 247
           KL+EK+ E+  I   N  L++R++ L VE   W+  A+ NE     L+ NL QV AQ   
Sbjct: 160 KLKEKDDEIVRIRNLNWVLQERVKSLYVENQIWRDIAQTNEANANTLRTNLDQVLAQLET 219

Query: 248 SKEGCGDSEVEDTA-----SCCNGRTLDFQLLCSNSNDVKELMYCKACRVNEVCMLLFPC 307
                  + VED A     SCC     +                CK C   E  +L+ PC
Sbjct: 220 FP--TASAVVEDDAESSCGSCCGDGGGEAVTAVGGG--------CKRCGEREASVLVLPC 279

Query: 308 KHLCLCKDC--ESRLTFCPVCQSSKFVGMEVYM 333
           +HLCLC  C   + L  CPVC       + V M
Sbjct: 280 RHLCLCTVCGGSALLRTCPVCDMVMNASVHVNM 302

BLAST of CsGy4G022500 vs. NCBI nr
Match: XP_004141264.1 (probable BOI-related E3 ubiquitin-protein ligase 2 isoform X2 [Cucumis sativus] >KGN55207.1 hypothetical protein Csa_012766 [Cucumis sativus])

HSP 1 Score: 668 bits (1724), Expect = 2.83e-242
Identity = 332/332 (100.00%), Postives = 332/332 (100.00%), Query Frame = 0

Query: 1   MALPQNHYQQHYQSQHQQPHQSISFRNLYTPDGHVSQPISYFNSFTLQDHSQHPPYVPPC 60
           MALPQNHYQQHYQSQHQQPHQSISFRNLYTPDGHVSQPISYFNSFTLQDHSQHPPYVPPC
Sbjct: 1   MALPQNHYQQHYQSQHQQPHQSISFRNLYTPDGHVSQPISYFNSFTLQDHSQHPPYVPPC 60

Query: 61  TAPGQDASDGVADLHWMYGIEPKKKRLKEQDLFENNSQISSIDFLQQRPVSTGLGLSLDN 120
           TAPGQDASDGVADLHWMYGIEPKKKRLKEQDLFENNSQISSIDFLQQRPVSTGLGLSLDN
Sbjct: 61  TAPGQDASDGVADLHWMYGIEPKKKRLKEQDLFENNSQISSIDFLQQRPVSTGLGLSLDN 120

Query: 121 TRMASTGDSPLVSLVGDDIDRELQQQDEEIEKFLKVQGDRLRHSILEKIQANQLQTLSIV 180
           TRMASTGDSPLVSLVGDDIDRELQQQDEEIEKFLKVQGDRLRHSILEKIQANQLQTLSIV
Sbjct: 121 TRMASTGDSPLVSLVGDDIDRELQQQDEEIEKFLKVQGDRLRHSILEKIQANQLQTLSIV 180

Query: 181 EEKIIKKLREKEAEVECINKKNIELEQRMEQLSVEAGAWQQRARYNENMITALKFNLQQV 240
           EEKIIKKLREKEAEVECINKKNIELEQRMEQLSVEAGAWQQRARYNENMITALKFNLQQV
Sbjct: 181 EEKIIKKLREKEAEVECINKKNIELEQRMEQLSVEAGAWQQRARYNENMITALKFNLQQV 240

Query: 241 YAQSRDSKEGCGDSEVEDTASCCNGRTLDFQLLCSNSNDVKELMYCKACRVNEVCMLLFP 300
           YAQSRDSKEGCGDSEVEDTASCCNGRTLDFQLLCSNSNDVKELMYCKACRVNEVCMLLFP
Sbjct: 241 YAQSRDSKEGCGDSEVEDTASCCNGRTLDFQLLCSNSNDVKELMYCKACRVNEVCMLLFP 300

Query: 301 CKHLCLCKDCESRLTFCPVCQSSKFVGMEVYM 332
           CKHLCLCKDCESRLTFCPVCQSSKFVGMEVYM
Sbjct: 301 CKHLCLCKDCESRLTFCPVCQSSKFVGMEVYM 332

BLAST of CsGy4G022500 vs. NCBI nr
Match: XP_011654117.1 (probable BOI-related E3 ubiquitin-protein ligase 2 isoform X3 [Cucumis sativus])

HSP 1 Score: 657 bits (1694), Expect = 9.83e-238
Identity = 329/332 (99.10%), Postives = 330/332 (99.40%), Query Frame = 0

Query: 1   MALPQNHYQQHYQSQHQQPHQSISFRNLYTPDGHVSQPISYFNSFTLQDHSQHPPYVPPC 60
           MALPQNHYQQHYQSQHQQPHQSISFRNLYTPDGHVSQPISYFNSFTLQDHSQHPPYVPP 
Sbjct: 1   MALPQNHYQQHYQSQHQQPHQSISFRNLYTPDGHVSQPISYFNSFTLQDHSQHPPYVPP- 60

Query: 61  TAPGQDASDGVADLHWMYGIEPKKKRLKEQDLFENNSQISSIDFLQQRPVSTGLGLSLDN 120
            +PGQDASDGVADLHWMYGIEPKKKRLKEQDLFENNSQISSIDFLQQRPVSTGLGLSLDN
Sbjct: 61  -SPGQDASDGVADLHWMYGIEPKKKRLKEQDLFENNSQISSIDFLQQRPVSTGLGLSLDN 120

Query: 121 TRMASTGDSPLVSLVGDDIDRELQQQDEEIEKFLKVQGDRLRHSILEKIQANQLQTLSIV 180
           TRMASTGDSPLVSLVGDDIDRELQQQDEEIEKFLKVQGDRLRHSILEKIQANQLQTLSIV
Sbjct: 121 TRMASTGDSPLVSLVGDDIDRELQQQDEEIEKFLKVQGDRLRHSILEKIQANQLQTLSIV 180

Query: 181 EEKIIKKLREKEAEVECINKKNIELEQRMEQLSVEAGAWQQRARYNENMITALKFNLQQV 240
           EEKIIKKLREKEAEVECINKKNIELEQRMEQLSVEAGAWQQRARYNENMITALKFNLQQV
Sbjct: 181 EEKIIKKLREKEAEVECINKKNIELEQRMEQLSVEAGAWQQRARYNENMITALKFNLQQV 240

Query: 241 YAQSRDSKEGCGDSEVEDTASCCNGRTLDFQLLCSNSNDVKELMYCKACRVNEVCMLLFP 300
           YAQSRDSKEGCGDSEVEDTASCCNGRTLDFQLLCSNSNDVKELMYCKACRVNEVCMLLFP
Sbjct: 241 YAQSRDSKEGCGDSEVEDTASCCNGRTLDFQLLCSNSNDVKELMYCKACRVNEVCMLLFP 300

Query: 301 CKHLCLCKDCESRLTFCPVCQSSKFVGMEVYM 332
           CKHLCLCKDCESRLTFCPVCQSSKFVGMEVYM
Sbjct: 301 CKHLCLCKDCESRLTFCPVCQSSKFVGMEVYM 330

BLAST of CsGy4G022500 vs. NCBI nr
Match: XP_031740606.1 (probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 [Cucumis sativus])

HSP 1 Score: 657 bits (1694), Expect = 1.43e-237
Identity = 331/340 (97.35%), Postives = 331/340 (97.35%), Query Frame = 0

Query: 1   MALPQNHYQQHYQSQHQQPHQSISFRNLYTPDGHVSQPISYFNSFTLQDHSQHPPYVPPC 60
           MALPQNHYQQHYQSQHQQPHQSISFRNLYTPDGHVSQPISYFNSFTLQDHSQHPPYVPP 
Sbjct: 1   MALPQNHYQQHYQSQHQQPHQSISFRNLYTPDGHVSQPISYFNSFTLQDHSQHPPYVPPF 60

Query: 61  --------TAPGQDASDGVADLHWMYGIEPKKKRLKEQDLFENNSQISSIDFLQQRPVST 120
                   TAPGQDASDGVADLHWMYGIEPKKKRLKEQDLFENNSQISSIDFLQQRPVST
Sbjct: 61  NVVGFAPGTAPGQDASDGVADLHWMYGIEPKKKRLKEQDLFENNSQISSIDFLQQRPVST 120

Query: 121 GLGLSLDNTRMASTGDSPLVSLVGDDIDRELQQQDEEIEKFLKVQGDRLRHSILEKIQAN 180
           GLGLSLDNTRMASTGDSPLVSLVGDDIDRELQQQDEEIEKFLKVQGDRLRHSILEKIQAN
Sbjct: 121 GLGLSLDNTRMASTGDSPLVSLVGDDIDRELQQQDEEIEKFLKVQGDRLRHSILEKIQAN 180

Query: 181 QLQTLSIVEEKIIKKLREKEAEVECINKKNIELEQRMEQLSVEAGAWQQRARYNENMITA 240
           QLQTLSIVEEKIIKKLREKEAEVECINKKNIELEQRMEQLSVEAGAWQQRARYNENMITA
Sbjct: 181 QLQTLSIVEEKIIKKLREKEAEVECINKKNIELEQRMEQLSVEAGAWQQRARYNENMITA 240

Query: 241 LKFNLQQVYAQSRDSKEGCGDSEVEDTASCCNGRTLDFQLLCSNSNDVKELMYCKACRVN 300
           LKFNLQQVYAQSRDSKEGCGDSEVEDTASCCNGRTLDFQLLCSNSNDVKELMYCKACRVN
Sbjct: 241 LKFNLQQVYAQSRDSKEGCGDSEVEDTASCCNGRTLDFQLLCSNSNDVKELMYCKACRVN 300

Query: 301 EVCMLLFPCKHLCLCKDCESRLTFCPVCQSSKFVGMEVYM 332
           EVCMLLFPCKHLCLCKDCESRLTFCPVCQSSKFVGMEVYM
Sbjct: 301 EVCMLLFPCKHLCLCKDCESRLTFCPVCQSSKFVGMEVYM 340

BLAST of CsGy4G022500 vs. NCBI nr
Match: XP_008452569.1 (PREDICTED: probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 [Cucumis melo] >KAA0064396.1 putative BOI-related E3 ubiquitin-protein ligase 2 isoform X1 [Cucumis melo var. makuwa] >TYK20191.1 putative BOI-related E3 ubiquitin-protein ligase 2 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 655 bits (1691), Expect = 3.04e-237
Identity = 328/332 (98.80%), Postives = 328/332 (98.80%), Query Frame = 0

Query: 1   MALPQNHYQQHYQSQHQQPHQSISFRNLYTPDGHVSQPISYFNSFTLQDHSQHPPYVPPC 60
           MALPQNHYQQHYQSQHQQ HQSISFRNLYT DGHVSQPISYFNSF LQDHSQHPPYVPPC
Sbjct: 1   MALPQNHYQQHYQSQHQQQHQSISFRNLYTADGHVSQPISYFNSFNLQDHSQHPPYVPPC 60

Query: 61  TAPGQDASDGVADLHWMYGIEPKKKRLKEQDLFENNSQISSIDFLQQRPVSTGLGLSLDN 120
           TAPGQDASDGVADLHWMYGIEPKKKRLKEQDLFENNSQISSIDFLQQRPVSTGLGLSLDN
Sbjct: 61  TAPGQDASDGVADLHWMYGIEPKKKRLKEQDLFENNSQISSIDFLQQRPVSTGLGLSLDN 120

Query: 121 TRMASTGDSPLVSLVGDDIDRELQQQDEEIEKFLKVQGDRLRHSILEKIQANQLQTLSIV 180
           TRMASTGDSPLVSLVGDDIDRELQQQDEEIEKFLKVQGDRLRHSILEKIQANQLQTLSIV
Sbjct: 121 TRMASTGDSPLVSLVGDDIDRELQQQDEEIEKFLKVQGDRLRHSILEKIQANQLQTLSIV 180

Query: 181 EEKIIKKLREKEAEVECINKKNIELEQRMEQLSVEAGAWQQRARYNENMITALKFNLQQV 240
           EEKIIKKLREKEAEVECINKKNIELEQRMEQLSVEAGAWQQRARYNENMITALKFNLQQV
Sbjct: 181 EEKIIKKLREKEAEVECINKKNIELEQRMEQLSVEAGAWQQRARYNENMITALKFNLQQV 240

Query: 241 YAQSRDSKEGCGDSEVEDTASCCNGRTLDFQLLCSNSNDVKELMYCKACRVNEVCMLLFP 300
           YAQSRDSKEGCGDSEVEDTASCCNGRTLDFQLL SNSNDVKELMYCKACRVNEVCMLLFP
Sbjct: 241 YAQSRDSKEGCGDSEVEDTASCCNGRTLDFQLLRSNSNDVKELMYCKACRVNEVCMLLFP 300

Query: 301 CKHLCLCKDCESRLTFCPVCQSSKFVGMEVYM 332
           CKHLCLCKDCESRLTFCPVCQSSKFVGMEVYM
Sbjct: 301 CKHLCLCKDCESRLTFCPVCQSSKFVGMEVYM 332

BLAST of CsGy4G022500 vs. NCBI nr
Match: XP_008452570.1 (PREDICTED: probable BOI-related E3 ubiquitin-protein ligase 2 isoform X2 [Cucumis melo])

HSP 1 Score: 644 bits (1661), Expect = 1.05e-232
Identity = 325/332 (97.89%), Postives = 326/332 (98.19%), Query Frame = 0

Query: 1   MALPQNHYQQHYQSQHQQPHQSISFRNLYTPDGHVSQPISYFNSFTLQDHSQHPPYVPPC 60
           MALPQNHYQQHYQSQHQQ HQSISFRNLYT DGHVSQPISYFNSF LQDHSQHPPYVPP 
Sbjct: 1   MALPQNHYQQHYQSQHQQQHQSISFRNLYTADGHVSQPISYFNSFNLQDHSQHPPYVPP- 60

Query: 61  TAPGQDASDGVADLHWMYGIEPKKKRLKEQDLFENNSQISSIDFLQQRPVSTGLGLSLDN 120
            +PGQDASDGVADLHWMYGIEPKKKRLKEQDLFENNSQISSIDFLQQRPVSTGLGLSLDN
Sbjct: 61  -SPGQDASDGVADLHWMYGIEPKKKRLKEQDLFENNSQISSIDFLQQRPVSTGLGLSLDN 120

Query: 121 TRMASTGDSPLVSLVGDDIDRELQQQDEEIEKFLKVQGDRLRHSILEKIQANQLQTLSIV 180
           TRMASTGDSPLVSLVGDDIDRELQQQDEEIEKFLKVQGDRLRHSILEKIQANQLQTLSIV
Sbjct: 121 TRMASTGDSPLVSLVGDDIDRELQQQDEEIEKFLKVQGDRLRHSILEKIQANQLQTLSIV 180

Query: 181 EEKIIKKLREKEAEVECINKKNIELEQRMEQLSVEAGAWQQRARYNENMITALKFNLQQV 240
           EEKIIKKLREKEAEVECINKKNIELEQRMEQLSVEAGAWQQRARYNENMITALKFNLQQV
Sbjct: 181 EEKIIKKLREKEAEVECINKKNIELEQRMEQLSVEAGAWQQRARYNENMITALKFNLQQV 240

Query: 241 YAQSRDSKEGCGDSEVEDTASCCNGRTLDFQLLCSNSNDVKELMYCKACRVNEVCMLLFP 300
           YAQSRDSKEGCGDSEVEDTASCCNGRTLDFQLL SNSNDVKELMYCKACRVNEVCMLLFP
Sbjct: 241 YAQSRDSKEGCGDSEVEDTASCCNGRTLDFQLLRSNSNDVKELMYCKACRVNEVCMLLFP 300

Query: 301 CKHLCLCKDCESRLTFCPVCQSSKFVGMEVYM 332
           CKHLCLCKDCESRLTFCPVCQSSKFVGMEVYM
Sbjct: 301 CKHLCLCKDCESRLTFCPVCQSSKFVGMEVYM 330

BLAST of CsGy4G022500 vs. ExPASy TrEMBL
Match: A0A0A0L3L7 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G639940 PE=4 SV=1)

HSP 1 Score: 668 bits (1724), Expect = 1.37e-242
Identity = 332/332 (100.00%), Postives = 332/332 (100.00%), Query Frame = 0

Query: 1   MALPQNHYQQHYQSQHQQPHQSISFRNLYTPDGHVSQPISYFNSFTLQDHSQHPPYVPPC 60
           MALPQNHYQQHYQSQHQQPHQSISFRNLYTPDGHVSQPISYFNSFTLQDHSQHPPYVPPC
Sbjct: 1   MALPQNHYQQHYQSQHQQPHQSISFRNLYTPDGHVSQPISYFNSFTLQDHSQHPPYVPPC 60

Query: 61  TAPGQDASDGVADLHWMYGIEPKKKRLKEQDLFENNSQISSIDFLQQRPVSTGLGLSLDN 120
           TAPGQDASDGVADLHWMYGIEPKKKRLKEQDLFENNSQISSIDFLQQRPVSTGLGLSLDN
Sbjct: 61  TAPGQDASDGVADLHWMYGIEPKKKRLKEQDLFENNSQISSIDFLQQRPVSTGLGLSLDN 120

Query: 121 TRMASTGDSPLVSLVGDDIDRELQQQDEEIEKFLKVQGDRLRHSILEKIQANQLQTLSIV 180
           TRMASTGDSPLVSLVGDDIDRELQQQDEEIEKFLKVQGDRLRHSILEKIQANQLQTLSIV
Sbjct: 121 TRMASTGDSPLVSLVGDDIDRELQQQDEEIEKFLKVQGDRLRHSILEKIQANQLQTLSIV 180

Query: 181 EEKIIKKLREKEAEVECINKKNIELEQRMEQLSVEAGAWQQRARYNENMITALKFNLQQV 240
           EEKIIKKLREKEAEVECINKKNIELEQRMEQLSVEAGAWQQRARYNENMITALKFNLQQV
Sbjct: 181 EEKIIKKLREKEAEVECINKKNIELEQRMEQLSVEAGAWQQRARYNENMITALKFNLQQV 240

Query: 241 YAQSRDSKEGCGDSEVEDTASCCNGRTLDFQLLCSNSNDVKELMYCKACRVNEVCMLLFP 300
           YAQSRDSKEGCGDSEVEDTASCCNGRTLDFQLLCSNSNDVKELMYCKACRVNEVCMLLFP
Sbjct: 241 YAQSRDSKEGCGDSEVEDTASCCNGRTLDFQLLCSNSNDVKELMYCKACRVNEVCMLLFP 300

Query: 301 CKHLCLCKDCESRLTFCPVCQSSKFVGMEVYM 332
           CKHLCLCKDCESRLTFCPVCQSSKFVGMEVYM
Sbjct: 301 CKHLCLCKDCESRLTFCPVCQSSKFVGMEVYM 332

BLAST of CsGy4G022500 vs. ExPASy TrEMBL
Match: A0A5A7VDE1 (Putative BOI-related E3 ubiquitin-protein ligase 2 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003250 PE=4 SV=1)

HSP 1 Score: 655 bits (1691), Expect = 1.47e-237
Identity = 328/332 (98.80%), Postives = 328/332 (98.80%), Query Frame = 0

Query: 1   MALPQNHYQQHYQSQHQQPHQSISFRNLYTPDGHVSQPISYFNSFTLQDHSQHPPYVPPC 60
           MALPQNHYQQHYQSQHQQ HQSISFRNLYT DGHVSQPISYFNSF LQDHSQHPPYVPPC
Sbjct: 1   MALPQNHYQQHYQSQHQQQHQSISFRNLYTADGHVSQPISYFNSFNLQDHSQHPPYVPPC 60

Query: 61  TAPGQDASDGVADLHWMYGIEPKKKRLKEQDLFENNSQISSIDFLQQRPVSTGLGLSLDN 120
           TAPGQDASDGVADLHWMYGIEPKKKRLKEQDLFENNSQISSIDFLQQRPVSTGLGLSLDN
Sbjct: 61  TAPGQDASDGVADLHWMYGIEPKKKRLKEQDLFENNSQISSIDFLQQRPVSTGLGLSLDN 120

Query: 121 TRMASTGDSPLVSLVGDDIDRELQQQDEEIEKFLKVQGDRLRHSILEKIQANQLQTLSIV 180
           TRMASTGDSPLVSLVGDDIDRELQQQDEEIEKFLKVQGDRLRHSILEKIQANQLQTLSIV
Sbjct: 121 TRMASTGDSPLVSLVGDDIDRELQQQDEEIEKFLKVQGDRLRHSILEKIQANQLQTLSIV 180

Query: 181 EEKIIKKLREKEAEVECINKKNIELEQRMEQLSVEAGAWQQRARYNENMITALKFNLQQV 240
           EEKIIKKLREKEAEVECINKKNIELEQRMEQLSVEAGAWQQRARYNENMITALKFNLQQV
Sbjct: 181 EEKIIKKLREKEAEVECINKKNIELEQRMEQLSVEAGAWQQRARYNENMITALKFNLQQV 240

Query: 241 YAQSRDSKEGCGDSEVEDTASCCNGRTLDFQLLCSNSNDVKELMYCKACRVNEVCMLLFP 300
           YAQSRDSKEGCGDSEVEDTASCCNGRTLDFQLL SNSNDVKELMYCKACRVNEVCMLLFP
Sbjct: 241 YAQSRDSKEGCGDSEVEDTASCCNGRTLDFQLLRSNSNDVKELMYCKACRVNEVCMLLFP 300

Query: 301 CKHLCLCKDCESRLTFCPVCQSSKFVGMEVYM 332
           CKHLCLCKDCESRLTFCPVCQSSKFVGMEVYM
Sbjct: 301 CKHLCLCKDCESRLTFCPVCQSSKFVGMEVYM 332

BLAST of CsGy4G022500 vs. ExPASy TrEMBL
Match: A0A1S3BVB4 (probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493554 PE=4 SV=1)

HSP 1 Score: 655 bits (1691), Expect = 1.47e-237
Identity = 328/332 (98.80%), Postives = 328/332 (98.80%), Query Frame = 0

Query: 1   MALPQNHYQQHYQSQHQQPHQSISFRNLYTPDGHVSQPISYFNSFTLQDHSQHPPYVPPC 60
           MALPQNHYQQHYQSQHQQ HQSISFRNLYT DGHVSQPISYFNSF LQDHSQHPPYVPPC
Sbjct: 1   MALPQNHYQQHYQSQHQQQHQSISFRNLYTADGHVSQPISYFNSFNLQDHSQHPPYVPPC 60

Query: 61  TAPGQDASDGVADLHWMYGIEPKKKRLKEQDLFENNSQISSIDFLQQRPVSTGLGLSLDN 120
           TAPGQDASDGVADLHWMYGIEPKKKRLKEQDLFENNSQISSIDFLQQRPVSTGLGLSLDN
Sbjct: 61  TAPGQDASDGVADLHWMYGIEPKKKRLKEQDLFENNSQISSIDFLQQRPVSTGLGLSLDN 120

Query: 121 TRMASTGDSPLVSLVGDDIDRELQQQDEEIEKFLKVQGDRLRHSILEKIQANQLQTLSIV 180
           TRMASTGDSPLVSLVGDDIDRELQQQDEEIEKFLKVQGDRLRHSILEKIQANQLQTLSIV
Sbjct: 121 TRMASTGDSPLVSLVGDDIDRELQQQDEEIEKFLKVQGDRLRHSILEKIQANQLQTLSIV 180

Query: 181 EEKIIKKLREKEAEVECINKKNIELEQRMEQLSVEAGAWQQRARYNENMITALKFNLQQV 240
           EEKIIKKLREKEAEVECINKKNIELEQRMEQLSVEAGAWQQRARYNENMITALKFNLQQV
Sbjct: 181 EEKIIKKLREKEAEVECINKKNIELEQRMEQLSVEAGAWQQRARYNENMITALKFNLQQV 240

Query: 241 YAQSRDSKEGCGDSEVEDTASCCNGRTLDFQLLCSNSNDVKELMYCKACRVNEVCMLLFP 300
           YAQSRDSKEGCGDSEVEDTASCCNGRTLDFQLL SNSNDVKELMYCKACRVNEVCMLLFP
Sbjct: 241 YAQSRDSKEGCGDSEVEDTASCCNGRTLDFQLLRSNSNDVKELMYCKACRVNEVCMLLFP 300

Query: 301 CKHLCLCKDCESRLTFCPVCQSSKFVGMEVYM 332
           CKHLCLCKDCESRLTFCPVCQSSKFVGMEVYM
Sbjct: 301 CKHLCLCKDCESRLTFCPVCQSSKFVGMEVYM 332

BLAST of CsGy4G022500 vs. ExPASy TrEMBL
Match: A0A1S3BTJ6 (probable BOI-related E3 ubiquitin-protein ligase 2 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103493554 PE=4 SV=1)

HSP 1 Score: 644 bits (1661), Expect = 5.11e-233
Identity = 325/332 (97.89%), Postives = 326/332 (98.19%), Query Frame = 0

Query: 1   MALPQNHYQQHYQSQHQQPHQSISFRNLYTPDGHVSQPISYFNSFTLQDHSQHPPYVPPC 60
           MALPQNHYQQHYQSQHQQ HQSISFRNLYT DGHVSQPISYFNSF LQDHSQHPPYVPP 
Sbjct: 1   MALPQNHYQQHYQSQHQQQHQSISFRNLYTADGHVSQPISYFNSFNLQDHSQHPPYVPP- 60

Query: 61  TAPGQDASDGVADLHWMYGIEPKKKRLKEQDLFENNSQISSIDFLQQRPVSTGLGLSLDN 120
            +PGQDASDGVADLHWMYGIEPKKKRLKEQDLFENNSQISSIDFLQQRPVSTGLGLSLDN
Sbjct: 61  -SPGQDASDGVADLHWMYGIEPKKKRLKEQDLFENNSQISSIDFLQQRPVSTGLGLSLDN 120

Query: 121 TRMASTGDSPLVSLVGDDIDRELQQQDEEIEKFLKVQGDRLRHSILEKIQANQLQTLSIV 180
           TRMASTGDSPLVSLVGDDIDRELQQQDEEIEKFLKVQGDRLRHSILEKIQANQLQTLSIV
Sbjct: 121 TRMASTGDSPLVSLVGDDIDRELQQQDEEIEKFLKVQGDRLRHSILEKIQANQLQTLSIV 180

Query: 181 EEKIIKKLREKEAEVECINKKNIELEQRMEQLSVEAGAWQQRARYNENMITALKFNLQQV 240
           EEKIIKKLREKEAEVECINKKNIELEQRMEQLSVEAGAWQQRARYNENMITALKFNLQQV
Sbjct: 181 EEKIIKKLREKEAEVECINKKNIELEQRMEQLSVEAGAWQQRARYNENMITALKFNLQQV 240

Query: 241 YAQSRDSKEGCGDSEVEDTASCCNGRTLDFQLLCSNSNDVKELMYCKACRVNEVCMLLFP 300
           YAQSRDSKEGCGDSEVEDTASCCNGRTLDFQLL SNSNDVKELMYCKACRVNEVCMLLFP
Sbjct: 241 YAQSRDSKEGCGDSEVEDTASCCNGRTLDFQLLRSNSNDVKELMYCKACRVNEVCMLLFP 300

Query: 301 CKHLCLCKDCESRLTFCPVCQSSKFVGMEVYM 332
           CKHLCLCKDCESRLTFCPVCQSSKFVGMEVYM
Sbjct: 301 CKHLCLCKDCESRLTFCPVCQSSKFVGMEVYM 330

BLAST of CsGy4G022500 vs. ExPASy TrEMBL
Match: A0A6J1FG60 (probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443616 PE=4 SV=1)

HSP 1 Score: 612 bits (1578), Expect = 2.28e-220
Identity = 303/332 (91.27%), Postives = 318/332 (95.78%), Query Frame = 0

Query: 1   MALPQNHYQQHYQSQHQQPHQSISFRNLYTPDGHVSQPISYFNSFTLQDHSQHPPYVPPC 60
           MALPQNHYQQHYQSQ Q  HQSISFRNLYT DGHVSQPISYFN F LQ+HSQHPPYVPPC
Sbjct: 1   MALPQNHYQQHYQSQQQ--HQSISFRNLYTADGHVSQPISYFNPFNLQEHSQHPPYVPPC 60

Query: 61  TAPGQDASDGVADLHWMYGIEPKKKRLKEQDLFENNSQISSIDFLQQRPVSTGLGLSLDN 120
           TAPGQDASDGVAD HWM+GIEPKKKRLKEQDLFENNSQISSIDFLQ RPVSTGLGLSLDN
Sbjct: 61  TAPGQDASDGVADFHWMHGIEPKKKRLKEQDLFENNSQISSIDFLQPRPVSTGLGLSLDN 120

Query: 121 TRMASTGDSPLVSLVGDDIDRELQQQDEEIEKFLKVQGDRLRHSILEKIQANQLQTLSIV 180
           TRMASTGDSPLVSL+GDDID ELQ+QDE+IEKFL VQG+RLRH+ILEKIQANQLQTLS V
Sbjct: 121 TRMASTGDSPLVSLIGDDIDLELQRQDEDIEKFLHVQGERLRHTILEKIQANQLQTLSTV 180

Query: 181 EEKIIKKLREKEAEVECINKKNIELEQRMEQLSVEAGAWQQRARYNENMITALKFNLQQV 240
           EE+IIKKLREKEAEVECINKKNIELEQRMEQLS+EAGAWQQRARYNENMITALKFNLQQV
Sbjct: 181 EERIIKKLREKEAEVECINKKNIELEQRMEQLSMEAGAWQQRARYNENMITALKFNLQQV 240

Query: 241 YAQSRDSKEGCGDSEVEDTASCCNGRTLDFQLLCSNSNDVKELMYCKACRVNEVCMLLFP 300
           YAQSRDSKEGCGDSEVEDTASCCNGR+LDFQLL  NSND+K+LMYCKACRVNEVCMLL P
Sbjct: 241 YAQSRDSKEGCGDSEVEDTASCCNGRSLDFQLLRGNSNDIKDLMYCKACRVNEVCMLLLP 300

Query: 301 CKHLCLCKDCESRLTFCPVCQSSKFVGMEVYM 332
           CKHLCLCKDCESRL+FCP+CQSSKF+GMEVYM
Sbjct: 301 CKHLCLCKDCESRLSFCPLCQSSKFIGMEVYM 330

BLAST of CsGy4G022500 vs. TAIR 10
Match: AT1G45976.1 (S-ribonuclease binding protein 1 )

HSP 1 Score: 366.7 bits (940), Expect = 2.0e-101
Identity = 192/329 (58.36%), Postives = 245/329 (74.47%), Query Frame = 0

Query: 16  HQQPHQSISFRNLYTPDGHVSQPISYFNSFTLQDHSQHPPYVPPC----TAPGQ----DA 75
           H     S +FR+    DG +S  + +  S  L D SQHPPY+PP      APG     D 
Sbjct: 4   HPHLQDSKNFRDFCGIDGQISPELGFNRSENLHDQSQHPPYIPPFHVAGFAPGPVVQIDG 63

Query: 76  SDG--VADLHWMY--GIEPKKKRLKEQDLFENNSQISSIDFLQQRPVSTGLGLSLDNTRM 135
           SDG   AD  W Y  G+EP+++R+KEQD  ENNSQISSIDFLQ R VSTGLGLSLDN R+
Sbjct: 64  SDGGNGADFEWNYGLGLEPRRERIKEQDFLENNSQISSIDFLQARSVSTGLGLSLDNARV 123

Query: 136 ASTGDSPLVSLVGDDIDRELQQQDEEIEKFLKVQGDRLRHSILEKIQANQLQTLSIVEEK 195
           AS+  S L+SLVGDDIDRELQ+QD +I++FLK+QGD+LRH+IL+KI+  Q +T+S++EEK
Sbjct: 124 ASSDGSALLSLVGDDIDRELQRQDADIDRFLKIQGDQLRHAILDKIKRGQQKTVSLMEEK 183

Query: 196 IIKKLREKEAEVECINKKNIELEQRMEQLSVEAGAWQQRARYNENMITALKFNLQQVYAQ 255
           +++KLREK+ E+E IN+KN ELE RMEQL++EA AWQQRA+YNENMI AL +NL +   +
Sbjct: 184 VVQKLREKDEELERINRKNKELEVRMEQLTMEAEAWQQRAKYNENMIAALNYNLDRAQGR 243

Query: 256 SRDSKEGCGDSEVEDTASCCNGRTLDFQLLCSNSNDVKELMYCKACRVNEVCMLLFPCKH 315
            RDS EGCGDSEV+DTASC NGR        +++N+ K +M C+ C V E+CMLL PC H
Sbjct: 244 PRDSIEGCGDSEVDDTASCFNGRD-------NSNNNTKTMMMCRFCGVREMCMLLLPCNH 303

Query: 316 LCLCKDCESRLTFCPVCQSSKFVGMEVYM 333
           +CLCK+CE +L+ CP+CQSSKF+GMEVYM
Sbjct: 304 MCLCKECERKLSSCPLCQSSKFLGMEVYM 325

BLAST of CsGy4G022500 vs. TAIR 10
Match: AT1G60610.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 151.4 bits (381), Expect = 1.3e-36
Identity = 94/259 (36.29%), Postives = 150/259 (57.92%), Query Frame = 0

Query: 93  FENNSQISSIDFLQQRPVSTGLGLSLD----NTRMASTGDS---PLVSLVGDDIDRELQQ 152
           + NN+ +   +  +Q  VSTGL LS D    N+ + S   S   P+   +GD+I  +L +
Sbjct: 88  YNNNNTVVQDEVPKQNLVSTGLRLSYDDDERNSSVTSANGSITTPVYQSLGDNIRLDLNR 147

Query: 153 QDEEIEKFLKVQGDRLRHSILEKIQANQLQTLSIVEEKIIKKLREKEAEVECINKKNIEL 212
           Q++E+++F+K + D++   + +  Q +    ++ +E+ + KKL+EK+ E+E +NKKN EL
Sbjct: 148 QNDELDQFIKFRADQMAKGVRDIKQRHVTSFVTALEKDVSKKLQEKDHEIESMNKKNREL 207

Query: 213 EQRMEQLSVEAGAWQQRARYNENMITALKFNLQQVYAQSRDS-------------KEGCG 272
             +++Q++VEA  W  +A+YNE+++ ALK NLQQV +   D+             KEG G
Sbjct: 208 VDKIKQVAVEAQNWHYKAKYNESVVNALKVNLQQVMSHGNDNNAVGGGVADHHQMKEGFG 267

Query: 273 DSEVEDTASCCNGRTLDFQLLCSNSNDVKELMYCKACRVNEVCMLLFPCKHLCLCKDCES 332
           DSE++D A+  N   L+   + S        M CK C V  V +LL PC+HL LCKDC+ 
Sbjct: 268 DSEIDDEAASYN--YLNIPGMPSTG------MRCKLCNVKNVSVLLVPCRHLSLCKDCDV 327

BLAST of CsGy4G022500 vs. TAIR 10
Match: AT1G60610.2 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 151.4 bits (381), Expect = 1.3e-36
Identity = 94/259 (36.29%), Postives = 150/259 (57.92%), Query Frame = 0

Query: 93  FENNSQISSIDFLQQRPVSTGLGLSLD----NTRMASTGDS---PLVSLVGDDIDRELQQ 152
           + NN+ +   +  +Q  VSTGL LS D    N+ + S   S   P+   +GD+I  +L +
Sbjct: 88  YNNNNTVVQDEVPKQNLVSTGLRLSYDDDERNSSVTSANGSITTPVYQSLGDNIRLDLNR 147

Query: 153 QDEEIEKFLKVQGDRLRHSILEKIQANQLQTLSIVEEKIIKKLREKEAEVECINKKNIEL 212
           Q++E+++F+K + D++   + +  Q +    ++ +E+ + KKL+EK+ E+E +NKKN EL
Sbjct: 148 QNDELDQFIKFRADQMAKGVRDIKQRHVTSFVTALEKDVSKKLQEKDHEIESMNKKNREL 207

Query: 213 EQRMEQLSVEAGAWQQRARYNENMITALKFNLQQVYAQSRDS-------------KEGCG 272
             +++Q++VEA  W  +A+YNE+++ ALK NLQQV +   D+             KEG G
Sbjct: 208 VDKIKQVAVEAQNWHYKAKYNESVVNALKVNLQQVMSHGNDNNAVGGGVADHHQMKEGFG 267

Query: 273 DSEVEDTASCCNGRTLDFQLLCSNSNDVKELMYCKACRVNEVCMLLFPCKHLCLCKDCES 332
           DSE++D A+  N   L+   + S        M CK C V  V +LL PC+HL LCKDC+ 
Sbjct: 268 DSEIDDEAASYN--YLNIPGMPSTG------MRCKLCNVKNVSVLLVPCRHLSLCKDCDV 327

BLAST of CsGy4G022500 vs. TAIR 10
Match: AT1G60610.3 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 151.4 bits (381), Expect = 1.3e-36
Identity = 94/259 (36.29%), Postives = 150/259 (57.92%), Query Frame = 0

Query: 93  FENNSQISSIDFLQQRPVSTGLGLSLD----NTRMASTGDS---PLVSLVGDDIDRELQQ 152
           + NN+ +   +  +Q  VSTGL LS D    N+ + S   S   P+   +GD+I  +L +
Sbjct: 88  YNNNNTVVQDEVPKQNLVSTGLRLSYDDDERNSSVTSANGSITTPVYQSLGDNIRLDLNR 147

Query: 153 QDEEIEKFLKVQGDRLRHSILEKIQANQLQTLSIVEEKIIKKLREKEAEVECINKKNIEL 212
           Q++E+++F+K + D++   + +  Q +    ++ +E+ + KKL+EK+ E+E +NKKN EL
Sbjct: 148 QNDELDQFIKFRADQMAKGVRDIKQRHVTSFVTALEKDVSKKLQEKDHEIESMNKKNREL 207

Query: 213 EQRMEQLSVEAGAWQQRARYNENMITALKFNLQQVYAQSRDS-------------KEGCG 272
             +++Q++VEA  W  +A+YNE+++ ALK NLQQV +   D+             KEG G
Sbjct: 208 VDKIKQVAVEAQNWHYKAKYNESVVNALKVNLQQVMSHGNDNNAVGGGVADHHQMKEGFG 267

Query: 273 DSEVEDTASCCNGRTLDFQLLCSNSNDVKELMYCKACRVNEVCMLLFPCKHLCLCKDCES 332
           DSE++D A+  N   L+   + S        M CK C V  V +LL PC+HL LCKDC+ 
Sbjct: 268 DSEIDDEAASYN--YLNIPGMPSTG------MRCKLCNVKNVSVLLVPCRHLSLCKDCDV 327

BLAST of CsGy4G022500 vs. TAIR 10
Match: AT1G10650.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 142.9 bits (359), Expect = 4.7e-34
Identity = 89/268 (33.21%), Postives = 152/268 (56.72%), Query Frame = 0

Query: 80  IEPKKKRLKEQDLFENNSQISSIDFLQQRPVSTGLGLSLDNTRMAST---------GDSP 139
           I+ ++++L+    +  N+     +  ++  VSTGL LS D+    S+           SP
Sbjct: 74  IQRQQQKLQMSLNYNYNNTSVREEVPKENLVSTGLRLSYDDDEHNSSVTSASGSILAASP 133

Query: 140 LVSLVGDDIDRELQQQDEEIEKFLKVQGDRLRHSILEKIQANQLQTLSIVEEKIIKKLRE 199
           +   + D +  +L +Q +E ++F+K+Q  ++   + +  Q +    L+ +E+ + KKL+E
Sbjct: 134 IFQSLDDSLRIDLHRQKDEFDQFIKIQAAQMAKGVRDMKQRHIASFLTTLEKGVSKKLQE 193

Query: 200 KEAEVECINKKNIELEQRMEQLSVEAGAWQQRARYNENMITALKFNLQQ-------VYAQ 259
           K+ E+  +NKKN EL +R++Q+++EA  W  RA+YNE+++  LK NLQQ       V A 
Sbjct: 194 KDHEINDMNKKNKELVERIKQVAMEAQNWHYRAKYNESVVNVLKANLQQAMSHNNSVIAA 253

Query: 260 SRDSKEGCGDSEVEDTASCCNGRTLDFQLLCSNSNDVKELMYCKACRVNEVCMLLFPCKH 319
           +   KEG GDSE++D AS      +D     +N+  + + M CK C V EV +L+ PC+H
Sbjct: 254 ADQGKEGFGDSEIDDAAS----SYIDPNNNNNNNMGIHQRMRCKMCNVKEVSVLIVPCRH 313

Query: 320 LCLCKDCESRLTFCPVCQSSKFVGMEVY 332
           L LCK+C+     CPVC+S K   ++V+
Sbjct: 314 LSLCKECDVFTKICPVCKSLKSSCVQVF 337

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4IDI63.8e-2530.88Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=37... [more]
Q9LDD11.2e-2131.75Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=37... [more]
Q9FHE46.2e-1529.17BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG... [more]
O818511.1e-1432.39E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_004141264.12.83e-242100.00probable BOI-related E3 ubiquitin-protein ligase 2 isoform X2 [Cucumis sativus] ... [more]
XP_011654117.19.83e-23899.10probable BOI-related E3 ubiquitin-protein ligase 2 isoform X3 [Cucumis sativus][more]
XP_031740606.11.43e-23797.35probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 [Cucumis sativus][more]
XP_008452569.13.04e-23798.80PREDICTED: probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 [Cucumi... [more]
XP_008452570.11.05e-23297.89PREDICTED: probable BOI-related E3 ubiquitin-protein ligase 2 isoform X2 [Cucumi... [more]
Match NameE-valueIdentityDescription
A0A0A0L3L71.37e-242100.00RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G639940 P... [more]
A0A5A7VDE11.47e-23798.80Putative BOI-related E3 ubiquitin-protein ligase 2 isoform X1 OS=Cucumis melo va... [more]
A0A1S3BVB41.47e-23798.80probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 OS=Cucumis melo OX... [more]
A0A1S3BTJ65.11e-23397.89probable BOI-related E3 ubiquitin-protein ligase 2 isoform X2 OS=Cucumis melo OX... [more]
A0A6J1FG602.28e-22091.27probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 OS=Cucurbita mosch... [more]
Match NameE-valueIdentityDescription
AT1G45976.12.0e-10158.36S-ribonuclease binding protein 1 [more]
AT1G60610.11.3e-3636.29SBP (S-ribonuclease binding protein) family protein [more]
AT1G60610.21.3e-3636.29SBP (S-ribonuclease binding protein) family protein [more]
AT1G60610.31.3e-3636.29SBP (S-ribonuclease binding protein) family protein [more]
AT1G10650.14.7e-3433.21SBP (S-ribonuclease binding protein) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 181..222
NoneNo IPR availablePFAMPF13920zf-C3HC4_3coord: 284..322
e-value: 6.9E-7
score: 29.0
NoneNo IPR availableGENE3D1.10.8.10coord: 140..159
e-value: 1.2E-8
score: 36.7
NoneNo IPR availableGENE3D1.10.1170.10coord: 295..321
e-value: 1.2E-8
score: 36.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availablePANTHERPTHR42647:SF10F2G19.2coord: 1..332
IPR017066E3 ubiquitin-protein ligase BOI-likePIRSFPIRSF036836SBP1_RNase_bindcoord: 9..332
e-value: 3.4E-89
score: 297.8
IPR017066E3 ubiquitin-protein ligase BOI-likePANTHERPTHR42647SBP (S-RIBONUCLEASE BINDING PROTEIN) FAMILY PROTEINcoord: 1..332
IPR011029Death-like domain superfamilyGENE3D1.10.533.10Death Domain, Fascoord: 160..294
e-value: 1.2E-8
score: 36.7
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 286..321
score: 9.081335

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy4G022500.1CsGy4G022500.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004842 ubiquitin-protein transferase activity