CsGy4G021530 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy4G021530
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionPolynucleotidyl transferase ribonuclease H-like superfamily protein
LocationGy14Chr4: 28177283 .. 28180317 (-)
RNA-Seq ExpressionCsGy4G021530
SyntenyCsGy4G021530
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAGCCCTCGAATTTGCTGCTCCAGAATTCGACCACTCTAACGTCCGTCGTCTTCCCCGGCTCCGGTCGACCGTCGGAGTGTGTACGTCATCCACTCACTTCCCTTCATTTCAATTCGATGTTTCTGAGAGCTTCGCTGCTTAAGTTTTCATCATTCTACTCAATCCCATGTCATCCTCTGCTTCCCGTCTCTCTCTCTTCCTCAATTTGTTCTTCTCCTCGCACACCTTTCTCTATCCGATGCCATTCAGCTTTCCCCTTATCCCACGAACCATCTTCTTCAGCTTCCTCTGAATTCCCCGATGTGGCGGATAATCGTTGGAGGCCCACGTGTTTGTATTTCACTCATGGCAGGTGCACAATGGTATTTTTCAATTCTGGTTTTAGTTGTAGCATGCGCGTTTTTCGAATGCTTTTATGTTTTATTTTCTCCCATTTTGTGAAATGATTGAAGATTCTACTTGACTGTGGATGCTAAGTTGTTGGTTTGGCGTTGTACACCACTTGTTCGACGATATTCCTCACAGAGCTTTTGCTCGTTTTTGCTGCGTGTTAAGCTGTCTAGTAGTTGATTTTTTATTAGGGACTCCATAATTTGAGAATTAGACGGGTAAGGTAATGGACATATCGTTTTAATTCTAAAATTGGATAATCTGTATCCCATCTGAACAAAATAGGAAATATTGGAATTCTATAAAAGTTTACGAATGTACAACCATTTTTCACATTTGATCAGGCTCTTGTCTTATGTTGTGTGTTCAGATGGTTTTCATGGATTTCCCCAGCTGAAATTTCGCAGAAAAATTTAGATATTTATTGTCTTATGTTGTGTAGATGGATGATCCACTCCACATAAAGAAGTTCAATCATGATATCTCCAGGGAGCTTCAGGTAGATGTTGCTAGGTCAAACCATATGCGAACTCAGGACTTTGATTATTTTCTGGTGCTTGATTTGGAGGGTAAAGTTGAAATTATTGAGTTTCCAGTCATATTAGTAAATGCCAAGACGATGGGTGTGACAGGTTTTTTCCACAGGTTCTACATGCTTCTTTATAACGTGCTCATTTTTGCTATTGGTTATGCATCAATTTCTTTTTTCACTTGGCAGATTGTGTATTTTATGCATTTCTATATTTCCCCGTTACCTGTTTTTCTTCTTAATAGACAATATAATCTCAAAGAGAATATAGAAGGAATTACCTGAGTATCCGCACTAATATATTAGATACTGATGCCTTACTACCCCTTTACCTTACATTTCTGATGGGCATATGAGTAATATAAGTATGGATGAGAGATGTCATTGATCGGCTTGTTATGTTTGTAACCCAATCATGCAAAATGATTTAACTTTCAAGAATCTCAACAAGTTCTTTGGGTAATTAAGAACAAGACATTTATATTTTTTCCTTTTTTGTTGCCATGTTTGCTGACGATTGCTTTAAATTCTTCCTCAGGTTTGTTAGGCCATCAAGAATGAGTGAACAGAGAATAAATGAATACATTGAAGGAAAATATGGCAAGTTTGGAGTTGACCGGTAGGCTTCTAGTCTTGTATGGGGAAGGCTTTATAATGTTTGACATTATTGTGAATTCATTTTGTTCTGAATTATTGATCAACCGTCAATCAAGGCCATGATAGACTTTTGGATGTATTTCACAATCTGATTGCATGACGTATTGATGTACATGTCCAATGCTAATTTGATAGGACAAGCTTTTCTGCAGTATGTTGACACCTCCTGTTAATATTCTACTGTAACTTTTCTTTACTAGCTGATAGTTCCATATTATAATTCACAGTGTCTGGCATGACACAGCCATACTGTACAAGGATGTTATTCAACAGTTCGAGGATTGGCTGGTTCAGCATCAGCTATGGAGAAAGGAACTGGGCGGTGTTCTTGATCGAGCGGCATTTGTGACATGGCAAGCCTCATTTTCTTGATCTACTTTTATTTTCCTTCCTAAATTGTTCATTTCTTTATATTGTTAATGTGGTATGTCTATCAGGAGTTTGCCCAGCAACCACTAGGTCTTTTCACCCTTTTATAACGTTAAGTTTTGATTATCTAATGATTTATTCTCTCTTTACTTCTACCAGTGGCAACTGGGATATAAAAACTCAGGTCCCACATCAATGTAGCGTGTCGAAGATGAAACTTCCTGACTATTTTATGGAGTGGATCAACATTAAAGATGTCTATCTTAACTTTTACACAAAAAAGGTACAACAATCCTGTCAAAATAACAGATCGTAGCATCTTGAGTGGCTTTATTATTCTGTTGTCAAGAGAGAAACACATTATTTTACTTTTAGGAGTCAGAATTATGAAATCAATCTGCTGCTGATCGTTTCACTTTTCTTCAACTGCTTGTATAGATTATATCACAAGCATTGCAAAACACAATACATTGGTTTAGAATGATCAAAACAGAGTTCAACATTCATTATTCCACACGGGAAAGACTTGAATGAAAATGATGTTCTTTGTCATACTTACACATAACATAATAATGATTAATGTAATTGATTGCTTTAGGTGGCTATAGTTCGCTACACTCCATAATTTGTTTAGTTTCTCTAAGATCAGATCTTACACGACCAATTATTGATGAAGTTGTTTACTTTGGATGGTAATTGGTAGGCAACGGGAATGGCGACAATGATGAGACAACTCGAAATGCCCTTGGTGGGAAGCCATCATCTTGGTATAGATGATACAAAGAATATCGTGAGAGTGTTGCAGAGAATGGTTGGTGATGGAGCACATTTGCAAGTCACTGCACGAAGGAATCCTAAATCTCCTCAACATGTTAAGTTTCTTTTTGAGAACCGTATTTAGTGATGGCTGTCTTCAGCAAGTTATCTTCCATATTTGTCTGGATGATCTATGGAATGGATAAGTCTTTAAAATAGATACTTCGATCTCATGAATTGTAGATGAATCTGTCAATTCAGTTCTGATGTGTTTCAAGTGTTGAAATTTGACCAGATGAAATATGTTGCAATTCTAAATTTGAAGTTTAGAGG

mRNA sequence

TAGCCCTCGAATTTGCTGCTCCAGAATTCGACCACTCTAACGTCCGTCGTCTTCCCCGGCTCCGGTCGACCGTCGGAGTGTGTACGTCATCCACTCACTTCCCTTCATTTCAATTCGATGTTTCTGAGAGCTTCGCTGCTTAAGTTTTCATCATTCTACTCAATCCCATGTCATCCTCTGCTTCCCGTCTCTCTCTCTTCCTCAATTTGTTCTTCTCCTCGCACACCTTTCTCTATCCGATGCCATTCAGCTTTCCCCTTATCCCACGAACCATCTTCTTCAGCTTCCTCTGAATTCCCCGATGTGGCGGATAATCGTTGGAGGCCCACGTGTTTGTATTTCACTCATGGCAGGTGCACAATGATGGATGATCCACTCCACATAAAGAAGTTCAATCATGATATCTCCAGGGAGCTTCAGGTAGATGTTGCTAGGTCAAACCATATGCGAACTCAGGACTTTGATTATTTTCTGGTGCTTGATTTGGAGGGTAAAGTTGAAATTATTGAGTTTCCAGTCATATTAGTAAATGCCAAGACGATGGGTGTGACAGGTTTTTTCCACAGGTTTGTTAGGCCATCAAGAATGAGTGAACAGAGAATAAATGAATACATTGAAGGAAAATATGGCAAGTTTGGAGTTGACCGTGTCTGGCATGACACAGCCATACTGTACAAGGATGTTATTCAACAGTTCGAGGATTGGCTGGTTCAGCATCAGCTATGGAGAAAGGAACTGGGCGGTGTTCTTGATCGAGCGGCATTTGTGACATGTGGCAACTGGGATATAAAAACTCAGGTCCCACATCAATGTAGCGTGTCGAAGATGAAACTTCCTGACTATTTTATGGAGTGGATCAACATTAAAGATGTCTATCTTAACTTTTACACAAAAAAGGCAACGGGAATGGCGACAATGATGAGACAACTCGAAATGCCCTTGGTGGGAAGCCATCATCTTGGTATAGATGATACAAAGAATATCGTGAGAGTGTTGCAGAGAATGGTTGGTGATGGAGCACATTTGCAAGTCACTGCACGAAGGAATCCTAAATCTCCTCAACATGTTAAGTTTCTTTTTGAGAACCGTATTTAGTGATGGCTGTCTTCAGCAAGTTATCTTCCATATTTGTCTGGATGATCTATGGAATGGATAAGTCTTTAAAATAGATACTTCGATCTCATGAATTGTAGATGAATCTGTCAATTCAGTTCTGATGTGTTTCAAGTGTTGAAATTTGACCAGATGAAATATGTTGCAATTCTAAATTTGAAGTTTAGAGG

Coding sequence (CDS)

ATGTTTCTGAGAGCTTCGCTGCTTAAGTTTTCATCATTCTACTCAATCCCATGTCATCCTCTGCTTCCCGTCTCTCTCTCTTCCTCAATTTGTTCTTCTCCTCGCACACCTTTCTCTATCCGATGCCATTCAGCTTTCCCCTTATCCCACGAACCATCTTCTTCAGCTTCCTCTGAATTCCCCGATGTGGCGGATAATCGTTGGAGGCCCACGTGTTTGTATTTCACTCATGGCAGGTGCACAATGATGGATGATCCACTCCACATAAAGAAGTTCAATCATGATATCTCCAGGGAGCTTCAGGTAGATGTTGCTAGGTCAAACCATATGCGAACTCAGGACTTTGATTATTTTCTGGTGCTTGATTTGGAGGGTAAAGTTGAAATTATTGAGTTTCCAGTCATATTAGTAAATGCCAAGACGATGGGTGTGACAGGTTTTTTCCACAGGTTTGTTAGGCCATCAAGAATGAGTGAACAGAGAATAAATGAATACATTGAAGGAAAATATGGCAAGTTTGGAGTTGACCGTGTCTGGCATGACACAGCCATACTGTACAAGGATGTTATTCAACAGTTCGAGGATTGGCTGGTTCAGCATCAGCTATGGAGAAAGGAACTGGGCGGTGTTCTTGATCGAGCGGCATTTGTGACATGTGGCAACTGGGATATAAAAACTCAGGTCCCACATCAATGTAGCGTGTCGAAGATGAAACTTCCTGACTATTTTATGGAGTGGATCAACATTAAAGATGTCTATCTTAACTTTTACACAAAAAAGGCAACGGGAATGGCGACAATGATGAGACAACTCGAAATGCCCTTGGTGGGAAGCCATCATCTTGGTATAGATGATACAAAGAATATCGTGAGAGTGTTGCAGAGAATGGTTGGTGATGGAGCACATTTGCAAGTCACTGCACGAAGGAATCCTAAATCTCCTCAACATGTTAAGTTTCTTTTTGAGAACCGTATTTAG

Protein sequence

MFLRASLLKFSSFYSIPCHPLLPVSLSSSICSSPRTPFSIRCHSAFPLSHEPSSSASSEFPDVADNRWRPTCLYFTHGRCTMMDDPLHIKKFNHDISRELQVDVARSNHMRTQDFDYFLVLDLEGKVEIIEFPVILVNAKTMGVTGFFHRFVRPSRMSEQRINEYIEGKYGKFGVDRVWHDTAILYKDVIQQFEDWLVQHQLWRKELGGVLDRAAFVTCGNWDIKTQVPHQCSVSKMKLPDYFMEWINIKDVYLNFYTKKATGMATMMRQLEMPLVGSHHLGIDDTKNIVRVLQRMVGDGAHLQVTARRNPKSPQHVKFLFENRI*
Homology
BLAST of CsGy4G021530 vs. ExPASy Swiss-Prot
Match: Q8W566 (Uncharacterized exonuclease domain-containing protein At3g15140 OS=Arabidopsis thaliana OX=3702 GN=At3g15140 PE=2 SV=1)

HSP 1 Score: 378.6 bits (971), Expect = 6.9e-104
Identity = 184/329 (55.93%), Postives = 241/329 (73.25%), Query Frame = 0

Query: 4   RASLLKFSSF----YSIPCHPLLPVSLSSSICSSPRTPFSIRCHSAFPLSHEPSSSASSE 63
           R SL + S F    +S P   L        +C+S  +       S F  S   SSS+ S 
Sbjct: 9   RVSLSRISPFRDTRFSYPA-TLALAHTKRIMCNSSHSVSPSPSPSDFSSSSSSSSSSPST 68

Query: 64  FP--DVADN-RWRPTCLYFTHGRCTMMDDPLHIKKFNHDISRELQVDVARSNHMRTQDFD 123
           F   + ++N RWRP CLY+THG+CT MDDP H++ FNHD S+EL+V  A     ++Q+F+
Sbjct: 69  FSLMETSENARWRPMCLYYTHGKCTKMDDPAHLEIFNHDCSKELRVAAADLERKKSQEFN 128

Query: 124 YFLVLDLEGKVEIIEFPVILVNAKTMGVTGFFHRFVRPSRMSEQRINEYIEGKYGKFGVD 183
           +FLV+DLEGKVEI+EFP+++V+AKTM V   FHRFVRP++MSEQ IN+YIEGKYG+ GVD
Sbjct: 129 FFLVIDLEGKVEILEFPILIVDAKTMEVVDLFHRFVRPTKMSEQAINKYIEGKYGELGVD 188

Query: 184 RVWHDTAILYKDVIQQFEDWLVQHQLWRKELGGVLDRAAFVTCGNWDIKTQVPHQCSVSK 243
           RVWHDTAI +K V+++FE WL +H LW K+    L+ AAFVTCGNWDIKT++P QC VS 
Sbjct: 189 RVWHDTAIPFKQVVEEFEVWLAEHDLWDKDTDWGLNDAAFVTCGNWDIKTKIPEQCVVSN 248

Query: 244 MKLPDYFMEWINIKDVYLNFYTKKATGMATMMRQLEMPLVGSHHLGIDDTKNIVRVLQRM 303
           + LP YFMEWIN+KDVYLNFY ++A GM +MMRQ  + L+GSHHLGIDDTKNI RV+QRM
Sbjct: 249 INLPPYFMEWINLKDVYLNFYGREARGMVSMMRQCGIKLMGSHHLGIDDTKNITRVVQRM 308

Query: 304 VGDGAHLQVTARRNPKSPQHVKFLFENRI 326
           + +GA L++TARR+  + ++V+FLF+NRI
Sbjct: 309 LSEGAVLKLTARRSKSNMRNVEFLFKNRI 336

BLAST of CsGy4G021530 vs. ExPASy Swiss-Prot
Match: Q5FVR4 (3'-5' exoribonuclease 1 OS=Rattus norvegicus OX=10116 GN=Eri1 PE=2 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.6e-23
Identity = 62/206 (30.10%), Postives = 107/206 (51.94%), Query Frame = 0

Query: 115 FDYFLVLDLEGKV----------EIIEFPVILVNAKTMGVTGFFHRFVRPSRMSEQRINE 174
           +DY  ++D E             EIIEFPV+L+N  ++ +   F ++VRP   S+  ++E
Sbjct: 123 YDYICIIDFEATCEEGNPAEFLHEIIEFPVVLLNTHSLEIEDTFQQYVRPEVNSQ--LSE 182

Query: 175 YIEGKYGKFGVDRVWHDTAILYKDVIQQFEDWLVQHQLWRKELGGVLDRAAFVTCGNWDI 234
           +     G  G+ +   D A  +  V+++  +W+   +L      G   +   +T G+WD+
Sbjct: 183 FC---IGLTGITQDQVDRADAFPQVLKKVIEWMKSKEL------GTKYKYCILTDGSWDM 242

Query: 235 KTQVPHQCSVSKMKLPDYFMEWINIKDVYLNFY--TKKATGMATMMRQLEMPLVGSHHLG 294
              +  QC +S++K P +  +WINI+  Y NFY   +  T +  M+ +L M   G  H G
Sbjct: 243 SKFLNIQCQLSRLKYPSFAKKWINIRKSYGNFYKVPRSQTKLTIMLEKLGMDYDGRPHSG 302

Query: 295 IDDTKNIVRVLQRMVGDGAHLQVTAR 309
           +DD+KNI R+  RM+ DG  L++  +
Sbjct: 303 LDDSKNIARIAVRMLQDGCELRINEK 317

BLAST of CsGy4G021530 vs. ExPASy Swiss-Prot
Match: Q7TMF2 (3'-5' exoribonuclease 1 OS=Mus musculus OX=10090 GN=Eri1 PE=1 SV=2)

HSP 1 Score: 110.5 bits (275), Expect = 3.5e-23
Identity = 62/206 (30.10%), Postives = 106/206 (51.46%), Query Frame = 0

Query: 115 FDYFLVLDLEGKV----------EIIEFPVILVNAKTMGVTGFFHRFVRPSRMSEQRINE 174
           +DY  ++D E             EIIEFPV+L+N  T+ +   F ++VRP      +++E
Sbjct: 123 YDYICIIDFEATCEEGNPAEFLHEIIEFPVVLLNTHTLEIEDTFQQYVRPE--VNAQLSE 182

Query: 175 YIEGKYGKFGVDRVWHDTAILYKDVIQQFEDWLVQHQLWRKELGGVLDRAAFVTCGNWDI 234
           +     G  G+ +   D A  +  V+++  +W+   +L      G   +   +T G+WD+
Sbjct: 183 FC---IGLTGITQDQVDRADAFPQVLKKVIEWMKSKEL------GTKYKYCILTDGSWDM 242

Query: 235 KTQVPHQCSVSKMKLPDYFMEWINIKDVYLNFY--TKKATGMATMMRQLEMPLVGSHHLG 294
              +  QC +S++K P +  +WINI+  Y NFY   +  T +  M+ +L M   G  H G
Sbjct: 243 SKFLSIQCRLSRLKHPAFAKKWINIRKSYGNFYKVPRSQTKLTIMLEKLGMDYDGRPHSG 302

Query: 295 IDDTKNIVRVLQRMVGDGAHLQVTAR 309
           +DD+KNI R+  RM+ DG  L++  +
Sbjct: 303 LDDSKNIARIAVRMLQDGCELRINEK 317

BLAST of CsGy4G021530 vs. ExPASy Swiss-Prot
Match: Q8IV48 (3'-5' exoribonuclease 1 OS=Homo sapiens OX=9606 GN=ERI1 PE=1 SV=3)

HSP 1 Score: 109.8 bits (273), Expect = 6.0e-23
Identity = 61/206 (29.61%), Postives = 106/206 (51.46%), Query Frame = 0

Query: 115 FDYFLVLDLEGKV----------EIIEFPVILVNAKTMGVTGFFHRFVRPSRMSEQRINE 174
           +DY  ++D E             EIIEFPV+L+N  T+ +   F ++VRP      ++++
Sbjct: 127 YDYICIIDFEATCEEGNPPEFVHEIIEFPVVLLNTHTLEIEDTFQQYVRPE--INTQLSD 186

Query: 175 YIEGKYGKFGVDRVWHDTAILYKDVIQQFEDWLVQHQLWRKELGGVLDRAAFVTCGNWDI 234
           +        G+ +   D A  +  V+++  DW+   +L      G   + + +T G+WD+
Sbjct: 187 FC---ISLTGITQDQVDRADTFPQVLKKVIDWMKLKEL------GTKYKYSLLTDGSWDM 246

Query: 235 KTQVPHQCSVSKMKLPDYFMEWINIKDVYLNFY--TKKATGMATMMRQLEMPLVGSHHLG 294
              +  QC +S++K P +  +WINI+  Y NFY   +  T +  M+ +L M   G  H G
Sbjct: 247 SKFLNIQCQLSRLKYPPFAKKWINIRKSYGNFYKVPRSQTKLTIMLEKLGMDYDGRPHCG 306

Query: 295 IDDTKNIVRVLQRMVGDGAHLQVTAR 309
           +DD+KNI R+  RM+ DG  L++  +
Sbjct: 307 LDDSKNIARIAVRMLQDGCELRINEK 321

BLAST of CsGy4G021530 vs. ExPASy Swiss-Prot
Match: A6QLH5 (ERI1 exoribonuclease 3 OS=Bos taurus OX=9913 GN=ERI3 PE=2 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 3.0e-22
Identity = 62/210 (29.52%), Postives = 107/210 (50.95%), Query Frame = 0

Query: 113 QDFDYFLVLDLEGKV--------EIIEFPVILVNAKTMGVTGFFHRFVRPSRMSEQRINE 172
           Q + YFLVLD E           EIIEFP++ +N +TM +   FH +V+P  +   ++  
Sbjct: 141 QRYHYFLVLDFEATCDKPQIHPQEIIEFPILKLNGRTMEIESTFHMYVQP--VVHPQLTP 200

Query: 173 YIEGKYGKFGVDRVWHDTAILYKDVIQQFEDWLVQHQLWRKELGGVLD---RAAFVTCGN 232
           +        G+ +   D     + V+++ ++W+ +         G+LD   ++ FVTCG+
Sbjct: 201 FCT---ELTGIIQAMVDGQPSLQQVLERVDEWMAKE--------GLLDPNVKSIFVTCGD 260

Query: 233 WDIKTQVPHQCSVSKMKLPDYFMEWINIKDVY---LNFYTKKATGMATMMRQLEMPLVGS 292
           WD+K  +P QC    + + DYF +WIN+K  Y   +  + K   G+  M + L +  +G 
Sbjct: 261 WDLKVMLPGQCQYLGLPVADYFKQWINLKKAYSFAMGCWPK--NGLLDMNKGLSLQHIGR 320

Query: 293 HHLGIDDTKNIVRVLQRMVGDGAHLQVTAR 309
            H GIDD KNI  +++ +   G   + T++
Sbjct: 321 PHSGIDDCKNIANIMKTLAYRGFIFKQTSK 335

BLAST of CsGy4G021530 vs. NCBI nr
Match: XP_004146041.1 (uncharacterized exonuclease domain-containing protein At3g15140 isoform X1 [Cucumis sativus] >KGN55096.1 hypothetical protein Csa_012433 [Cucumis sativus])

HSP 1 Score: 674 bits (1739), Expect = 8.46e-245
Identity = 325/325 (100.00%), Postives = 325/325 (100.00%), Query Frame = 0

Query: 1   MFLRASLLKFSSFYSIPCHPLLPVSLSSSICSSPRTPFSIRCHSAFPLSHEPSSSASSEF 60
           MFLRASLLKFSSFYSIPCHPLLPVSLSSSICSSPRTPFSIRCHSAFPLSHEPSSSASSEF
Sbjct: 1   MFLRASLLKFSSFYSIPCHPLLPVSLSSSICSSPRTPFSIRCHSAFPLSHEPSSSASSEF 60

Query: 61  PDVADNRWRPTCLYFTHGRCTMMDDPLHIKKFNHDISRELQVDVARSNHMRTQDFDYFLV 120
           PDVADNRWRPTCLYFTHGRCTMMDDPLHIKKFNHDISRELQVDVARSNHMRTQDFDYFLV
Sbjct: 61  PDVADNRWRPTCLYFTHGRCTMMDDPLHIKKFNHDISRELQVDVARSNHMRTQDFDYFLV 120

Query: 121 LDLEGKVEIIEFPVILVNAKTMGVTGFFHRFVRPSRMSEQRINEYIEGKYGKFGVDRVWH 180
           LDLEGKVEIIEFPVILVNAKTMGVTGFFHRFVRPSRMSEQRINEYIEGKYGKFGVDRVWH
Sbjct: 121 LDLEGKVEIIEFPVILVNAKTMGVTGFFHRFVRPSRMSEQRINEYIEGKYGKFGVDRVWH 180

Query: 181 DTAILYKDVIQQFEDWLVQHQLWRKELGGVLDRAAFVTCGNWDIKTQVPHQCSVSKMKLP 240
           DTAILYKDVIQQFEDWLVQHQLWRKELGGVLDRAAFVTCGNWDIKTQVPHQCSVSKMKLP
Sbjct: 181 DTAILYKDVIQQFEDWLVQHQLWRKELGGVLDRAAFVTCGNWDIKTQVPHQCSVSKMKLP 240

Query: 241 DYFMEWINIKDVYLNFYTKKATGMATMMRQLEMPLVGSHHLGIDDTKNIVRVLQRMVGDG 300
           DYFMEWINIKDVYLNFYTKKATGMATMMRQLEMPLVGSHHLGIDDTKNIVRVLQRMVGDG
Sbjct: 241 DYFMEWINIKDVYLNFYTKKATGMATMMRQLEMPLVGSHHLGIDDTKNIVRVLQRMVGDG 300

Query: 301 AHLQVTARRNPKSPQHVKFLFENRI 325
           AHLQVTARRNPKSPQHVKFLFENRI
Sbjct: 301 AHLQVTARRNPKSPQHVKFLFENRI 325

BLAST of CsGy4G021530 vs. NCBI nr
Match: XP_008463707.1 (PREDICTED: uncharacterized exonuclease domain-containing protein At3g15140 isoform X1 [Cucumis melo])

HSP 1 Score: 646 bits (1666), Expect = 1.14e-233
Identity = 311/325 (95.69%), Postives = 316/325 (97.23%), Query Frame = 0

Query: 1   MFLRASLLKFSSFYSIPCHPLLPVSLSSSICSSPRTPFSIRCHSAFPLSHEPSSSASSEF 60
           MFLRASLLK SSFYSIPCHPLLPVSLSSSICSSPRTPFSIRCHSA P+SHEPSSSA S+F
Sbjct: 1   MFLRASLLKLSSFYSIPCHPLLPVSLSSSICSSPRTPFSIRCHSALPISHEPSSSAPSQF 60

Query: 61  PDVADNRWRPTCLYFTHGRCTMMDDPLHIKKFNHDISRELQVDVARSNHMRTQDFDYFLV 120
           PDVADNRWRPTCLYFTHGRCTMMDDPLHIKKFNHDISRELQVDVARSNHMRTQD DYFLV
Sbjct: 61  PDVADNRWRPTCLYFTHGRCTMMDDPLHIKKFNHDISRELQVDVARSNHMRTQDVDYFLV 120

Query: 121 LDLEGKVEIIEFPVILVNAKTMGVTGFFHRFVRPSRMSEQRINEYIEGKYGKFGVDRVWH 180
           LDLEGKVEIIEFPVILVNAKTMGVTGFFHRFVRPSRMSE+RINEYIEGKYGKFGVDRVWH
Sbjct: 121 LDLEGKVEIIEFPVILVNAKTMGVTGFFHRFVRPSRMSEKRINEYIEGKYGKFGVDRVWH 180

Query: 181 DTAILYKDVIQQFEDWLVQHQLWRKELGGVLDRAAFVTCGNWDIKTQVPHQCSVSKMKLP 240
           DTAILY DVIQQFEDWLVQHQLWRKE GGVLDRAAFVTCGNWDIKTQVPHQC+VSKMKLP
Sbjct: 181 DTAILYNDVIQQFEDWLVQHQLWRKERGGVLDRAAFVTCGNWDIKTQVPHQCNVSKMKLP 240

Query: 241 DYFMEWINIKDVYLNFYTKKATGMATMMRQLEMPLVGSHHLGIDDTKNIVRVLQRMVGDG 300
           DYFMEWINIKDVYLNFY KKATGMATMMRQLEM LVGSHHLGIDDTKNIVRVLQRMVGDG
Sbjct: 241 DYFMEWINIKDVYLNFYGKKATGMATMMRQLEMCLVGSHHLGIDDTKNIVRVLQRMVGDG 300

Query: 301 AHLQVTARRNPKSPQHVKFLFENRI 325
           AHLQ+TARRNPKSPQ VKFLFENRI
Sbjct: 301 AHLQITARRNPKSPQQVKFLFENRI 325

BLAST of CsGy4G021530 vs. NCBI nr
Match: XP_038896828.1 (uncharacterized exonuclease domain-containing protein At3g15140 [Benincasa hispida])

HSP 1 Score: 619 bits (1595), Expect = 7.30e-223
Identity = 297/325 (91.38%), Postives = 307/325 (94.46%), Query Frame = 0

Query: 1   MFLRASLLKFSSFYSIPCHPLLPVSLSSSICSSPRTPFSIRCHSAFPLSHEPSSSASSEF 60
           MFLRA LLK S  YSIPCH L+P+SLSSSICSS RTPF +RCHSA P+SHEPSSS  SEF
Sbjct: 1   MFLRAPLLKLSPIYSIPCHSLVPLSLSSSICSS-RTPFDLRCHSALPISHEPSSSPPSEF 60

Query: 61  PDVADNRWRPTCLYFTHGRCTMMDDPLHIKKFNHDISRELQVDVARSNHMRTQDFDYFLV 120
           PDVADNRWRPTCLYFT G+CTMMDDPLHIKKFNHDIS ELQVDVARSNHMR QD D+FLV
Sbjct: 61  PDVADNRWRPTCLYFTQGKCTMMDDPLHIKKFNHDISMELQVDVARSNHMRIQDVDFFLV 120

Query: 121 LDLEGKVEIIEFPVILVNAKTMGVTGFFHRFVRPSRMSEQRINEYIEGKYGKFGVDRVWH 180
           LDLEGKVEIIEFPVILVNAKTMGVTGFFHRFVRPSRMSEQRINEYIEGKYGKFGVDRVWH
Sbjct: 121 LDLEGKVEIIEFPVILVNAKTMGVTGFFHRFVRPSRMSEQRINEYIEGKYGKFGVDRVWH 180

Query: 181 DTAILYKDVIQQFEDWLVQHQLWRKELGGVLDRAAFVTCGNWDIKTQVPHQCSVSKMKLP 240
           DTAIL+KDVIQ+FEDWLVQHQLWRKELGGVLDRAAFVTCGNWDIKTQ PHQCSVSKMKLP
Sbjct: 181 DTAILFKDVIQEFEDWLVQHQLWRKELGGVLDRAAFVTCGNWDIKTQAPHQCSVSKMKLP 240

Query: 241 DYFMEWINIKDVYLNFYTKKATGMATMMRQLEMPLVGSHHLGIDDTKNIVRVLQRMVGDG 300
           DYFMEWINIKDVYLNFY KKATGMATMMRQLE+PLVGSHHLGIDDTKNIVRV+QRMV DG
Sbjct: 241 DYFMEWINIKDVYLNFYNKKATGMATMMRQLEIPLVGSHHLGIDDTKNIVRVMQRMVADG 300

Query: 301 AHLQVTARRNPKSPQHVKFLFENRI 325
           AHLQ TARRNPKSPQHVKFLFENRI
Sbjct: 301 AHLQTTARRNPKSPQHVKFLFENRI 324

BLAST of CsGy4G021530 vs. NCBI nr
Match: XP_022935722.1 (uncharacterized exonuclease domain-containing protein At3g15140 [Cucurbita moschata])

HSP 1 Score: 595 bits (1535), Expect = 1.06e-213
Identity = 284/325 (87.38%), Postives = 300/325 (92.31%), Query Frame = 0

Query: 1   MFLRASLLKFSSFYSIPCHPLLPVSLSSSICSSPRTPFSIRCHSAFPLSHEPSSSASSEF 60
           MF+RA LLK SSFYSIPCH L+P+ LSS+ICSSPRTP SIRCHS+ P +H+PSSSA SE 
Sbjct: 1   MFVRAPLLKVSSFYSIPCHSLVPLYLSSAICSSPRTPLSIRCHSSLPTAHDPSSSAPSES 60

Query: 61  PDVADNRWRPTCLYFTHGRCTMMDDPLHIKKFNHDISRELQVDVARSNHMRTQDFDYFLV 120
            DV  NRWRP CLY+T  +CTMMDDPLHIKKFNHDISRELQVDVA S HMRTQD ++FLV
Sbjct: 61  LDVVHNRWRPMCLYYTQSKCTMMDDPLHIKKFNHDISRELQVDVASSKHMRTQDIEFFLV 120

Query: 121 LDLEGKVEIIEFPVILVNAKTMGVTGFFHRFVRPSRMSEQRINEYIEGKYGKFGVDRVWH 180
           LDLEGKVEIIEFPVILVNAKT+ VTGFFHRFVRPSRMSE+RINEYIEGKYGKFGVDRVWH
Sbjct: 121 LDLEGKVEIIEFPVILVNAKTLDVTGFFHRFVRPSRMSERRINEYIEGKYGKFGVDRVWH 180

Query: 181 DTAILYKDVIQQFEDWLVQHQLWRKELGGVLDRAAFVTCGNWDIKTQVPHQCSVSKMKLP 240
           DTAILYKDVIQQFEDWLV+HQLWRKELGGVLDRAAFVTCGNWDIKTQVPHQCSVS MKLP
Sbjct: 181 DTAILYKDVIQQFEDWLVEHQLWRKELGGVLDRAAFVTCGNWDIKTQVPHQCSVSNMKLP 240

Query: 241 DYFMEWINIKDVYLNFYTKKATGMATMMRQLEMPLVGSHHLGIDDTKNIVRVLQRMVGDG 300
           DYFMEWINIKDVYLNFY KKATGM TMMRQLE+PLVGSHHLGIDDTKNIVRVLQRMV DG
Sbjct: 241 DYFMEWINIKDVYLNFYQKKATGMTTMMRQLEIPLVGSHHLGIDDTKNIVRVLQRMVADG 300

Query: 301 AHLQVTARRNPKSPQHVKFLFENRI 325
           AHLQ+TARRNP SPQHVKF FENRI
Sbjct: 301 AHLQITARRNPNSPQHVKFRFENRI 325

BLAST of CsGy4G021530 vs. NCBI nr
Match: XP_022976542.1 (uncharacterized exonuclease domain-containing protein At3g15140 [Cucurbita maxima])

HSP 1 Score: 595 bits (1533), Expect = 2.13e-213
Identity = 283/325 (87.08%), Postives = 301/325 (92.62%), Query Frame = 0

Query: 1   MFLRASLLKFSSFYSIPCHPLLPVSLSSSICSSPRTPFSIRCHSAFPLSHEPSSSASSEF 60
           MF+RA LLK SSFYSIPCH L+P+SLSS+ICSSPRTP +IRCHS+ P +HEPSSSA SE 
Sbjct: 1   MFVRAPLLKVSSFYSIPCHSLVPLSLSSAICSSPRTPLAIRCHSSLPTAHEPSSSAPSES 60

Query: 61  PDVADNRWRPTCLYFTHGRCTMMDDPLHIKKFNHDISRELQVDVARSNHMRTQDFDYFLV 120
            DV  NRWRP CLY+T  +CTMMDDPLHIKKFNHDISRELQVDVA S HMRTQD ++FLV
Sbjct: 61  LDVVHNRWRPMCLYYTQSKCTMMDDPLHIKKFNHDISRELQVDVASSKHMRTQDIEFFLV 120

Query: 121 LDLEGKVEIIEFPVILVNAKTMGVTGFFHRFVRPSRMSEQRINEYIEGKYGKFGVDRVWH 180
           LDLEGKVEIIEFPVILVNAKT+ VTGFFHRFVRPSRMSE+RINEYIEGKYGKFGVDRVWH
Sbjct: 121 LDLEGKVEIIEFPVILVNAKTLDVTGFFHRFVRPSRMSERRINEYIEGKYGKFGVDRVWH 180

Query: 181 DTAILYKDVIQQFEDWLVQHQLWRKELGGVLDRAAFVTCGNWDIKTQVPHQCSVSKMKLP 240
           DTAILYKDVIQQFEDWLV+HQ+WRK+LGGVLDRAAFVTCGNWDIKTQVPHQCSVS MKLP
Sbjct: 181 DTAILYKDVIQQFEDWLVEHQIWRKDLGGVLDRAAFVTCGNWDIKTQVPHQCSVSNMKLP 240

Query: 241 DYFMEWINIKDVYLNFYTKKATGMATMMRQLEMPLVGSHHLGIDDTKNIVRVLQRMVGDG 300
           DYFMEWINIKDVYLNFY KKATGMA MMRQLE+PLVGSHHLGIDDTKNIVRVLQRMV DG
Sbjct: 241 DYFMEWINIKDVYLNFYQKKATGMAMMMRQLEIPLVGSHHLGIDDTKNIVRVLQRMVADG 300

Query: 301 AHLQVTARRNPKSPQHVKFLFENRI 325
           AHLQ+TARRNP SPQHVKF FENRI
Sbjct: 301 AHLQITARRNPNSPQHVKFHFENRI 325

BLAST of CsGy4G021530 vs. ExPASy TrEMBL
Match: A0A0A0L4N2 (Exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G628930 PE=4 SV=1)

HSP 1 Score: 674 bits (1739), Expect = 4.09e-245
Identity = 325/325 (100.00%), Postives = 325/325 (100.00%), Query Frame = 0

Query: 1   MFLRASLLKFSSFYSIPCHPLLPVSLSSSICSSPRTPFSIRCHSAFPLSHEPSSSASSEF 60
           MFLRASLLKFSSFYSIPCHPLLPVSLSSSICSSPRTPFSIRCHSAFPLSHEPSSSASSEF
Sbjct: 1   MFLRASLLKFSSFYSIPCHPLLPVSLSSSICSSPRTPFSIRCHSAFPLSHEPSSSASSEF 60

Query: 61  PDVADNRWRPTCLYFTHGRCTMMDDPLHIKKFNHDISRELQVDVARSNHMRTQDFDYFLV 120
           PDVADNRWRPTCLYFTHGRCTMMDDPLHIKKFNHDISRELQVDVARSNHMRTQDFDYFLV
Sbjct: 61  PDVADNRWRPTCLYFTHGRCTMMDDPLHIKKFNHDISRELQVDVARSNHMRTQDFDYFLV 120

Query: 121 LDLEGKVEIIEFPVILVNAKTMGVTGFFHRFVRPSRMSEQRINEYIEGKYGKFGVDRVWH 180
           LDLEGKVEIIEFPVILVNAKTMGVTGFFHRFVRPSRMSEQRINEYIEGKYGKFGVDRVWH
Sbjct: 121 LDLEGKVEIIEFPVILVNAKTMGVTGFFHRFVRPSRMSEQRINEYIEGKYGKFGVDRVWH 180

Query: 181 DTAILYKDVIQQFEDWLVQHQLWRKELGGVLDRAAFVTCGNWDIKTQVPHQCSVSKMKLP 240
           DTAILYKDVIQQFEDWLVQHQLWRKELGGVLDRAAFVTCGNWDIKTQVPHQCSVSKMKLP
Sbjct: 181 DTAILYKDVIQQFEDWLVQHQLWRKELGGVLDRAAFVTCGNWDIKTQVPHQCSVSKMKLP 240

Query: 241 DYFMEWINIKDVYLNFYTKKATGMATMMRQLEMPLVGSHHLGIDDTKNIVRVLQRMVGDG 300
           DYFMEWINIKDVYLNFYTKKATGMATMMRQLEMPLVGSHHLGIDDTKNIVRVLQRMVGDG
Sbjct: 241 DYFMEWINIKDVYLNFYTKKATGMATMMRQLEMPLVGSHHLGIDDTKNIVRVLQRMVGDG 300

Query: 301 AHLQVTARRNPKSPQHVKFLFENRI 325
           AHLQVTARRNPKSPQHVKFLFENRI
Sbjct: 301 AHLQVTARRNPKSPQHVKFLFENRI 325

BLAST of CsGy4G021530 vs. ExPASy TrEMBL
Match: A0A1S3CJV9 (uncharacterized exonuclease domain-containing protein At3g15140 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501789 PE=4 SV=1)

HSP 1 Score: 646 bits (1666), Expect = 5.52e-234
Identity = 311/325 (95.69%), Postives = 316/325 (97.23%), Query Frame = 0

Query: 1   MFLRASLLKFSSFYSIPCHPLLPVSLSSSICSSPRTPFSIRCHSAFPLSHEPSSSASSEF 60
           MFLRASLLK SSFYSIPCHPLLPVSLSSSICSSPRTPFSIRCHSA P+SHEPSSSA S+F
Sbjct: 1   MFLRASLLKLSSFYSIPCHPLLPVSLSSSICSSPRTPFSIRCHSALPISHEPSSSAPSQF 60

Query: 61  PDVADNRWRPTCLYFTHGRCTMMDDPLHIKKFNHDISRELQVDVARSNHMRTQDFDYFLV 120
           PDVADNRWRPTCLYFTHGRCTMMDDPLHIKKFNHDISRELQVDVARSNHMRTQD DYFLV
Sbjct: 61  PDVADNRWRPTCLYFTHGRCTMMDDPLHIKKFNHDISRELQVDVARSNHMRTQDVDYFLV 120

Query: 121 LDLEGKVEIIEFPVILVNAKTMGVTGFFHRFVRPSRMSEQRINEYIEGKYGKFGVDRVWH 180
           LDLEGKVEIIEFPVILVNAKTMGVTGFFHRFVRPSRMSE+RINEYIEGKYGKFGVDRVWH
Sbjct: 121 LDLEGKVEIIEFPVILVNAKTMGVTGFFHRFVRPSRMSEKRINEYIEGKYGKFGVDRVWH 180

Query: 181 DTAILYKDVIQQFEDWLVQHQLWRKELGGVLDRAAFVTCGNWDIKTQVPHQCSVSKMKLP 240
           DTAILY DVIQQFEDWLVQHQLWRKE GGVLDRAAFVTCGNWDIKTQVPHQC+VSKMKLP
Sbjct: 181 DTAILYNDVIQQFEDWLVQHQLWRKERGGVLDRAAFVTCGNWDIKTQVPHQCNVSKMKLP 240

Query: 241 DYFMEWINIKDVYLNFYTKKATGMATMMRQLEMPLVGSHHLGIDDTKNIVRVLQRMVGDG 300
           DYFMEWINIKDVYLNFY KKATGMATMMRQLEM LVGSHHLGIDDTKNIVRVLQRMVGDG
Sbjct: 241 DYFMEWINIKDVYLNFYGKKATGMATMMRQLEMCLVGSHHLGIDDTKNIVRVLQRMVGDG 300

Query: 301 AHLQVTARRNPKSPQHVKFLFENRI 325
           AHLQ+TARRNPKSPQ VKFLFENRI
Sbjct: 301 AHLQITARRNPKSPQQVKFLFENRI 325

BLAST of CsGy4G021530 vs. ExPASy TrEMBL
Match: A0A6J1F6E0 (uncharacterized exonuclease domain-containing protein At3g15140 OS=Cucurbita moschata OX=3662 GN=LOC111442549 PE=4 SV=1)

HSP 1 Score: 595 bits (1535), Expect = 5.11e-214
Identity = 284/325 (87.38%), Postives = 300/325 (92.31%), Query Frame = 0

Query: 1   MFLRASLLKFSSFYSIPCHPLLPVSLSSSICSSPRTPFSIRCHSAFPLSHEPSSSASSEF 60
           MF+RA LLK SSFYSIPCH L+P+ LSS+ICSSPRTP SIRCHS+ P +H+PSSSA SE 
Sbjct: 1   MFVRAPLLKVSSFYSIPCHSLVPLYLSSAICSSPRTPLSIRCHSSLPTAHDPSSSAPSES 60

Query: 61  PDVADNRWRPTCLYFTHGRCTMMDDPLHIKKFNHDISRELQVDVARSNHMRTQDFDYFLV 120
            DV  NRWRP CLY+T  +CTMMDDPLHIKKFNHDISRELQVDVA S HMRTQD ++FLV
Sbjct: 61  LDVVHNRWRPMCLYYTQSKCTMMDDPLHIKKFNHDISRELQVDVASSKHMRTQDIEFFLV 120

Query: 121 LDLEGKVEIIEFPVILVNAKTMGVTGFFHRFVRPSRMSEQRINEYIEGKYGKFGVDRVWH 180
           LDLEGKVEIIEFPVILVNAKT+ VTGFFHRFVRPSRMSE+RINEYIEGKYGKFGVDRVWH
Sbjct: 121 LDLEGKVEIIEFPVILVNAKTLDVTGFFHRFVRPSRMSERRINEYIEGKYGKFGVDRVWH 180

Query: 181 DTAILYKDVIQQFEDWLVQHQLWRKELGGVLDRAAFVTCGNWDIKTQVPHQCSVSKMKLP 240
           DTAILYKDVIQQFEDWLV+HQLWRKELGGVLDRAAFVTCGNWDIKTQVPHQCSVS MKLP
Sbjct: 181 DTAILYKDVIQQFEDWLVEHQLWRKELGGVLDRAAFVTCGNWDIKTQVPHQCSVSNMKLP 240

Query: 241 DYFMEWINIKDVYLNFYTKKATGMATMMRQLEMPLVGSHHLGIDDTKNIVRVLQRMVGDG 300
           DYFMEWINIKDVYLNFY KKATGM TMMRQLE+PLVGSHHLGIDDTKNIVRVLQRMV DG
Sbjct: 241 DYFMEWINIKDVYLNFYQKKATGMTTMMRQLEIPLVGSHHLGIDDTKNIVRVLQRMVADG 300

Query: 301 AHLQVTARRNPKSPQHVKFLFENRI 325
           AHLQ+TARRNP SPQHVKF FENRI
Sbjct: 301 AHLQITARRNPNSPQHVKFRFENRI 325

BLAST of CsGy4G021530 vs. ExPASy TrEMBL
Match: A0A6J1IG11 (uncharacterized exonuclease domain-containing protein At3g15140 OS=Cucurbita maxima OX=3661 GN=LOC111476907 PE=4 SV=1)

HSP 1 Score: 595 bits (1533), Expect = 1.03e-213
Identity = 283/325 (87.08%), Postives = 301/325 (92.62%), Query Frame = 0

Query: 1   MFLRASLLKFSSFYSIPCHPLLPVSLSSSICSSPRTPFSIRCHSAFPLSHEPSSSASSEF 60
           MF+RA LLK SSFYSIPCH L+P+SLSS+ICSSPRTP +IRCHS+ P +HEPSSSA SE 
Sbjct: 1   MFVRAPLLKVSSFYSIPCHSLVPLSLSSAICSSPRTPLAIRCHSSLPTAHEPSSSAPSES 60

Query: 61  PDVADNRWRPTCLYFTHGRCTMMDDPLHIKKFNHDISRELQVDVARSNHMRTQDFDYFLV 120
            DV  NRWRP CLY+T  +CTMMDDPLHIKKFNHDISRELQVDVA S HMRTQD ++FLV
Sbjct: 61  LDVVHNRWRPMCLYYTQSKCTMMDDPLHIKKFNHDISRELQVDVASSKHMRTQDIEFFLV 120

Query: 121 LDLEGKVEIIEFPVILVNAKTMGVTGFFHRFVRPSRMSEQRINEYIEGKYGKFGVDRVWH 180
           LDLEGKVEIIEFPVILVNAKT+ VTGFFHRFVRPSRMSE+RINEYIEGKYGKFGVDRVWH
Sbjct: 121 LDLEGKVEIIEFPVILVNAKTLDVTGFFHRFVRPSRMSERRINEYIEGKYGKFGVDRVWH 180

Query: 181 DTAILYKDVIQQFEDWLVQHQLWRKELGGVLDRAAFVTCGNWDIKTQVPHQCSVSKMKLP 240
           DTAILYKDVIQQFEDWLV+HQ+WRK+LGGVLDRAAFVTCGNWDIKTQVPHQCSVS MKLP
Sbjct: 181 DTAILYKDVIQQFEDWLVEHQIWRKDLGGVLDRAAFVTCGNWDIKTQVPHQCSVSNMKLP 240

Query: 241 DYFMEWINIKDVYLNFYTKKATGMATMMRQLEMPLVGSHHLGIDDTKNIVRVLQRMVGDG 300
           DYFMEWINIKDVYLNFY KKATGMA MMRQLE+PLVGSHHLGIDDTKNIVRVLQRMV DG
Sbjct: 241 DYFMEWINIKDVYLNFYQKKATGMAMMMRQLEIPLVGSHHLGIDDTKNIVRVLQRMVADG 300

Query: 301 AHLQVTARRNPKSPQHVKFLFENRI 325
           AHLQ+TARRNP SPQHVKF FENRI
Sbjct: 301 AHLQITARRNPNSPQHVKFHFENRI 325

BLAST of CsGy4G021530 vs. ExPASy TrEMBL
Match: A0A6J1CCI3 (uncharacterized exonuclease domain-containing protein At3g15140 OS=Momordica charantia OX=3673 GN=LOC111010332 PE=4 SV=1)

HSP 1 Score: 562 bits (1449), Expect = 6.99e-201
Identity = 272/328 (82.93%), Postives = 297/328 (90.55%), Query Frame = 0

Query: 1   MFLRASLLKFSSFYSIPCHPLLPVSLSSSICSSPRTPFSIRCHSAFPLSHEPSSSASSEF 60
           MFLRA LLK SSFYSIPC+PL+P+SL SS+ SS RTPF IRCH++ P +HEPSSSA    
Sbjct: 1   MFLRAPLLKVSSFYSIPCYPLVPLSLYSSVFSSSRTPFPIRCHASLPAAHEPSSSAPPPH 60

Query: 61  -PDVADNRWRPTCLYFTHGRCTMMDDPLHIKKFNHDISRELQVDVARSNHMRTQ--DFDY 120
            P+ ADNRWRP CLY+T G+CTMMDDPLH+KKFNHDISR LQV+ ARSNH RTQ  D D+
Sbjct: 61  SPEAADNRWRPMCLYYTQGKCTMMDDPLHLKKFNHDISRVLQVN-ARSNHTRTQSQDIDF 120

Query: 121 FLVLDLEGKVEIIEFPVILVNAKTMGVTGFFHRFVRPSRMSEQRINEYIEGKYGKFGVDR 180
           FLVLDLEGKVEIIEFPVILV+AKT+GVTGFFHRFVRPS MSE+RINEYIEGKYGKFGVDR
Sbjct: 121 FLVLDLEGKVEIIEFPVILVSAKTLGVTGFFHRFVRPSNMSERRINEYIEGKYGKFGVDR 180

Query: 181 VWHDTAILYKDVIQQFEDWLVQHQLWRKELGGVLDRAAFVTCGNWDIKTQVPHQCSVSKM 240
           VWHDTAI YK+VIQQFEDWLV+HQLW+KELGG LDRAAFVTCGNWDIKTQVPHQCSVS +
Sbjct: 181 VWHDTAISYKEVIQQFEDWLVEHQLWKKELGGCLDRAAFVTCGNWDIKTQVPHQCSVSNI 240

Query: 241 KLPDYFMEWINIKDVYLNFYTKKATGMATMMRQLEMPLVGSHHLGIDDTKNIVRVLQRMV 300
           +LP+YFMEWINIKDVYLNFY KKATGM TMMRQLE+PL+GSHHLGIDDTKNIVRVLQRMV
Sbjct: 241 ELPNYFMEWINIKDVYLNFYQKKATGMTTMMRQLEIPLLGSHHLGIDDTKNIVRVLQRMV 300

Query: 301 GDGAHLQVTARRNPKSPQHVKFLFENRI 325
            DGA LQ+TARRNP SPQHVKFLFENRI
Sbjct: 301 ADGARLQITARRNPNSPQHVKFLFENRI 327

BLAST of CsGy4G021530 vs. TAIR 10
Match: AT3G15140.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 378.6 bits (971), Expect = 4.9e-105
Identity = 184/329 (55.93%), Postives = 241/329 (73.25%), Query Frame = 0

Query: 4   RASLLKFSSF----YSIPCHPLLPVSLSSSICSSPRTPFSIRCHSAFPLSHEPSSSASSE 63
           R SL + S F    +S P   L        +C+S  +       S F  S   SSS+ S 
Sbjct: 9   RVSLSRISPFRDTRFSYPA-TLALAHTKRIMCNSSHSVSPSPSPSDFSSSSSSSSSSPST 68

Query: 64  FP--DVADN-RWRPTCLYFTHGRCTMMDDPLHIKKFNHDISRELQVDVARSNHMRTQDFD 123
           F   + ++N RWRP CLY+THG+CT MDDP H++ FNHD S+EL+V  A     ++Q+F+
Sbjct: 69  FSLMETSENARWRPMCLYYTHGKCTKMDDPAHLEIFNHDCSKELRVAAADLERKKSQEFN 128

Query: 124 YFLVLDLEGKVEIIEFPVILVNAKTMGVTGFFHRFVRPSRMSEQRINEYIEGKYGKFGVD 183
           +FLV+DLEGKVEI+EFP+++V+AKTM V   FHRFVRP++MSEQ IN+YIEGKYG+ GVD
Sbjct: 129 FFLVIDLEGKVEILEFPILIVDAKTMEVVDLFHRFVRPTKMSEQAINKYIEGKYGELGVD 188

Query: 184 RVWHDTAILYKDVIQQFEDWLVQHQLWRKELGGVLDRAAFVTCGNWDIKTQVPHQCSVSK 243
           RVWHDTAI +K V+++FE WL +H LW K+    L+ AAFVTCGNWDIKT++P QC VS 
Sbjct: 189 RVWHDTAIPFKQVVEEFEVWLAEHDLWDKDTDWGLNDAAFVTCGNWDIKTKIPEQCVVSN 248

Query: 244 MKLPDYFMEWINIKDVYLNFYTKKATGMATMMRQLEMPLVGSHHLGIDDTKNIVRVLQRM 303
           + LP YFMEWIN+KDVYLNFY ++A GM +MMRQ  + L+GSHHLGIDDTKNI RV+QRM
Sbjct: 249 INLPPYFMEWINLKDVYLNFYGREARGMVSMMRQCGIKLMGSHHLGIDDTKNITRVVQRM 308

Query: 304 VGDGAHLQVTARRNPKSPQHVKFLFENRI 326
           + +GA L++TARR+  + ++V+FLF+NRI
Sbjct: 309 LSEGAVLKLTARRSKSNMRNVEFLFKNRI 336

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8W5666.9e-10455.93Uncharacterized exonuclease domain-containing protein At3g15140 OS=Arabidopsis t... [more]
Q5FVR41.6e-2330.103'-5' exoribonuclease 1 OS=Rattus norvegicus OX=10116 GN=Eri1 PE=2 SV=1[more]
Q7TMF23.5e-2330.103'-5' exoribonuclease 1 OS=Mus musculus OX=10090 GN=Eri1 PE=1 SV=2[more]
Q8IV486.0e-2329.613'-5' exoribonuclease 1 OS=Homo sapiens OX=9606 GN=ERI1 PE=1 SV=3[more]
A6QLH53.0e-2229.52ERI1 exoribonuclease 3 OS=Bos taurus OX=9913 GN=ERI3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_004146041.18.46e-245100.00uncharacterized exonuclease domain-containing protein At3g15140 isoform X1 [Cucu... [more]
XP_008463707.11.14e-23395.69PREDICTED: uncharacterized exonuclease domain-containing protein At3g15140 isofo... [more]
XP_038896828.17.30e-22391.38uncharacterized exonuclease domain-containing protein At3g15140 [Benincasa hispi... [more]
XP_022935722.11.06e-21387.38uncharacterized exonuclease domain-containing protein At3g15140 [Cucurbita mosch... [more]
XP_022976542.12.13e-21387.08uncharacterized exonuclease domain-containing protein At3g15140 [Cucurbita maxim... [more]
Match NameE-valueIdentityDescription
A0A0A0L4N24.09e-245100.00Exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G628930... [more]
A0A1S3CJV95.52e-23495.69uncharacterized exonuclease domain-containing protein At3g15140 isoform X1 OS=Cu... [more]
A0A6J1F6E05.11e-21487.38uncharacterized exonuclease domain-containing protein At3g15140 OS=Cucurbita mos... [more]
A0A6J1IG111.03e-21387.08uncharacterized exonuclease domain-containing protein At3g15140 OS=Cucurbita max... [more]
A0A6J1CCI36.99e-20182.93uncharacterized exonuclease domain-containing protein At3g15140 OS=Momordica cha... [more]
Match NameE-valueIdentityDescription
AT3G15140.14.9e-10555.93Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 105..323
e-value: 1.0E-57
score: 197.1
IPR013520Exonuclease, RNase T/DNA polymerase IIIPFAMPF00929RNase_Tcoord: 122..293
e-value: 2.3E-9
score: 38.0
NoneNo IPR availablePANTHERPTHR230443'-5' EXONUCLEASE ERI1-RELATEDcoord: 23..325
NoneNo IPR availablePANTHERPTHR23044:SF68OS06G0353400 PROTEINcoord: 23..325
NoneNo IPR availableCDDcd06133ERI-1_3'hExo_likecoord: 118..296
e-value: 1.53957E-49
score: 161.234
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 113..304

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy4G021530.2CsGy4G021530.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000467 exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
molecular_function GO:0000175 3'-5'-exoribonuclease activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0016740 transferase activity