CsGy4G021320 (gene) Cucumber (Gy14) v2.1

BLAST of CsGy4G021320 vs. ExPASy Swiss-Prot
Match: P81898 (Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A OS=Prunus dulcis OX=3755 PE=1 SV=2)

HSP 1 Score: 747.3 bits (1928), Expect = 1.4e-214
Identity = 374/563 (66.43%), Postives = 435/563 (77.26%), Query Frame = 0

Query: 49  HSTPPTVFFEVSKPIVTPKSKPCSLLLLHHDFAFTYGKPPVLADYTPPSHCSFTKFSKIV 108
           H TPPTVFFEV+KPI  PK+KPCS L+L HDFA+TYG+ PV A+YTPPS C    FS IV
Sbjct: 6   HDTPPTVFFEVTKPIEVPKTKPCSQLILQHDFAYTYGQAPVFANYTPPSDCPSQTFSTIV 65

Query: 109 LEWKATCRGRQFDRIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYSSLLSKNQTLA 168
           LEWKATCR RQFDRIFGVWLGGVEILRSCTAEPR  GIVWTV+KDITRY SLL  NQTLA
Sbjct: 66  LEWKATCRRRQFDRIFGVWLGGVEILRSCTAEPRPNGIVWTVEKDITRYYSLLKSNQTLA 125

Query: 169 VYLGNLVDKTYTGIYNVKIRIHFYPEEEGFGGNGVYSQKLGSAYDSDSRADLILPISRNF 228
           VYLGNL+DKTYTGIY+V I +HFYP +E           L S Y   S ADLILPISRN 
Sbjct: 126 VYLGNLIDKTYTGIYHVNISLHFYPAKEKLNSFQQKLDNLASGY--HSWADLILPISRNL 185

Query: 229 PLNDGLWFEVQNSTDVEFKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLT 288
           PLNDGLWFEVQNS D E KEF+IPQN YRAVLEVYVSFHENDEFWYSNLPN+YI ANNL+
Sbjct: 186 PLNDGLWFEVQNSNDTELKEFKIPQNAYRAVLEVYVSFHENDEFWYSNLPNEYIAANNLS 245

Query: 289 DTPGNGPFREVLVDLDGEIVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPF 348
            TPGNGPFREV+V LDGE+VGAVWPFTVI+TGG+NPLLWRPI+ IGSF+LP+YDIE+TPF
Sbjct: 246 GTPGNGPFREVVVSLDGEVVGAVWPFTVIFTGGINPLLWRPITAIGSFDLPTYDIEITPF 305

Query: 349 LGNLLDGKVHNFGFRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNF 408
           LG +LDGK H FGF VT+ALNVW+++ANLHLWLD  S KTE KL  H      +S   +F
Sbjct: 306 LGKILDGKSHKFGFNVTNALNVWYVDANLHLWLDKQSTKTEGKLSKHSSLPLVVSLVSDF 365

Query: 409 TGPDGTFLTKVTRSVSSAGWVKSSFGIITTLSNQDLSYSNSMTMGNNGSSQTVNQEIQFN 468
            G +GTFLT+ +RSVSS GWVKSS+G ITT S QD  YSNSM +G +G+ Q VNQ+I FN
Sbjct: 366 KGLNGTFLTRTSRSVSSTGWVKSSYGNITTRSIQDFYYSNSMVLGKDGNMQIVNQKIIFN 425

Query: 469 TSVYAKKESSHVYSVKSLKTFPLYMYSNTKDEENGSYQSIANLTLGFNEKKTDGLGKVIS 528
            SVY    SS+V+S+ S KTFPLY+Y++   + NG+Y  I N+ LGF EKK+ GLG   S
Sbjct: 426 DSVYINLPSSYVHSLTSHKTFPLYLYTDFLGQGNGTYLLITNVDLGFIEKKS-GLGFSNS 485

Query: 529 SLKNVQNGQGLIVVKGHLVTSGLGSTQQDYRYHDDVQCYSRNISSSNYTILHDNVKNTCG 588
           SL+N+++ +G +VVK +LV SGL STQQ YRY     CY RNISSSNYTIL+D V + C 
Sbjct: 486 SLRNLRSAEGNMVVKNNLVVSGLESTQQIYRYDGGKFCYFRNISSSNYTILYDKVGSKCN 545

Query: 589 EKQ-NSPESLRFRRWPIPARRAF 611
           +K  ++ + +  R WP  AR  F
Sbjct: 546 KKSLSNLDFVLSRLWPFGARMNF 565

BLAST of CsGy4G021320 vs. NCBI nr
Match: XP_004146092.2 (peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A [Cucumis sativus] >KGN55069.1 hypothetical protein Csa_011983 [Cucumis sativus])

HSP 1 Score: 1250 bits (3234), Expect = 0.0
Identity = 620/622 (99.68%), Postives = 620/622 (99.68%), Query Frame = 0

Query: 1   MASSFLLLISLLLLLLLLLPFHSNANLHKTRLRRSDLFSEPDVTTTADHSTPPTVFFEVS 60
           MASSFLLLISLLLLLLLLLPFHSNANLHKTRLRRSDLFSEPDVTTTADHSTPPTVFFEVS
Sbjct: 1   MASSFLLLISLLLLLLLLLPFHSNANLHKTRLRRSDLFSEPDVTTTADHSTPPTVFFEVS 60

Query: 61  KPIVTPKSKPCSLLLLHHDFAFTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQF 120
           KPIVTPKSKPCSLLLLHHDFAFTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQF
Sbjct: 61  KPIVTPKSKPCSLLLLHHDFAFTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQF 120

Query: 121 DRIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYSSLLSKNQTLAVYLGNLVDKTYT 180
           DRIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYSSLLSKNQTLAVYLGNLVDKTYT
Sbjct: 121 DRIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYSSLLSKNQTLAVYLGNLVDKTYT 180

Query: 181 GIYNVKIRIHFYPEEEGFGGNGVYSQKLGSAYDSDSRADLILPISRNFPLNDGLWFEVQN 240
           GIYNVKIRIHFYPEEEGFGGNGVYSQKLGSAYDSDSRADLILPISRN PLNDGLWFEVQN
Sbjct: 181 GIYNVKIRIHFYPEEEGFGGNGVYSQKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQN 240

Query: 241 STDVEFKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVL 300
           STDVEFKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVL
Sbjct: 241 STDVEFKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVL 300

Query: 301 VDLDGEIVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKVHNF 360
           VDLDGEIVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKVHNF
Sbjct: 301 VDLDGEIVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKVHNF 360

Query: 361 GFRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGPDGTFLTKVT 420
           GFRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGPDGTFLTKVT
Sbjct: 361 GFRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGPDGTFLTKVT 420

Query: 421 RSVSSAGWVKSSFGIITTLSNQDLSYSNSMTMGNNGSSQTVNQEIQFNTSVYAKKESSHV 480
           RSVSS GWVKSSFGIITTLSNQDLSYSNSMTMGNNGSSQTVNQEIQFNTSVYAKKESSHV
Sbjct: 421 RSVSSTGWVKSSFGIITTLSNQDLSYSNSMTMGNNGSSQTVNQEIQFNTSVYAKKESSHV 480

Query: 481 YSVKSLKTFPLYMYSNTKDEENGSYQSIANLTLGFNEKKTDGLGKVISSLKNVQNGQGLI 540
           YSVKSLKTFPLYMYSNTKDEENGSYQSIANLTLGFNEKKTDGLGKVISSLKNVQNGQGLI
Sbjct: 481 YSVKSLKTFPLYMYSNTKDEENGSYQSIANLTLGFNEKKTDGLGKVISSLKNVQNGQGLI 540

Query: 541 VVKGHLVTSGLGSTQQDYRYHDDVQCYSRNISSSNYTILHDNVKNTCGEKQNSPESLRFR 600
           VVKGHLVTSGLGSTQQDYRYHDDVQCYSRNISSSNYTILHDNVKNTCGEKQNSPESLRFR
Sbjct: 541 VVKGHLVTSGLGSTQQDYRYHDDVQCYSRNISSSNYTILHDNVKNTCGEKQNSPESLRFR 600

Query: 601 RWPIPARRAFLDSNLFVNNGRV 622
           RWPIPARRAFLDSNLFVNNGRV
Sbjct: 601 RWPIPARRAFLDSNLFVNNGRV 622

BLAST of CsGy4G021320 vs. NCBI nr
Match: XP_008463736.1 (PREDICTED: peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A [Cucumis melo] >KAA0066768.1 peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A [Cucumis melo var. makuwa] >TYK27915.1 peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A [Cucumis melo var. makuwa])

HSP 1 Score: 1158 bits (2995), Expect = 0.0
Identity = 572/621 (92.11%), Postives = 597/621 (96.14%), Query Frame = 0

Query: 2   ASSFLLLISLLLLLLLLLPFHSNANLHKTRLRRSDLFSEPDVTTTADHSTPPTVFFEVSK 61
           +SSFLLLISLLLLLL   PFHS+A+LHKTRLR+SDLFSE  V+TTADHSTPPTVFFEV+K
Sbjct: 3   SSSFLLLISLLLLLL---PFHSDADLHKTRLRQSDLFSESHVSTTADHSTPPTVFFEVTK 62

Query: 62  PIVTPKSKPCSLLLLHHDFAFTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFD 121
           PIVTPK+KPCSLL+LHHDFA+TYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFD
Sbjct: 63  PIVTPKAKPCSLLVLHHDFAYTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFD 122

Query: 122 RIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYSSLLSKNQTLAVYLGNLVDKTYTG 181
           RIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRY+SLLSKNQTLAVYLGNLVDKTYTG
Sbjct: 123 RIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLLSKNQTLAVYLGNLVDKTYTG 182

Query: 182 IYNVKIRIHFYPEEEGFGGNGVYSQKLGSAYDSDSRADLILPISRNFPLNDGLWFEVQNS 241
           IYNVKI IHFYPEEEGFGGNGVYS+KLGSAYDSDSRADLILPISRN PLNDGLWFEVQNS
Sbjct: 183 IYNVKIHIHFYPEEEGFGGNGVYSEKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNS 242

Query: 242 TDVEFKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVLV 301
           TDV+ KEFEIPQNVYRA+LEVYVSFHENDEFWYSNLPNDYI+ANNLTDTPGNGPFREVLV
Sbjct: 243 TDVQLKEFEIPQNVYRALLEVYVSFHENDEFWYSNLPNDYIVANNLTDTPGNGPFREVLV 302

Query: 302 DLDGEIVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKVHNFG 361
           DLDG+IV AVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGK H+FG
Sbjct: 303 DLDGDIVAAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKGHSFG 362

Query: 362 FRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGPDGTFLTKVTR 421
           FRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTG DGTFLTKVTR
Sbjct: 363 FRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGADGTFLTKVTR 422

Query: 422 SVSSAGWVKSSFGIITTLSNQDLSYSNSMTMGNNGSSQTVNQEIQFNTSVYAKKESSHVY 481
           SVSSAGWVKSSFG ITTLSNQDLSYSN M MGNNGSSQTVNQEI FN+SVYAK+ESSHVY
Sbjct: 423 SVSSAGWVKSSFGTITTLSNQDLSYSNLMMMGNNGSSQTVNQEIHFNSSVYAKQESSHVY 482

Query: 482 SVKSLKTFPLYMYSNTKDEENGSYQSIANLTLGFNEKKTDGLGKVISSLKNVQNGQGLIV 541
           S K+LKTFPLYMYSNT D+ NGSY+SIANLTLGFNEKKTDGLG  ISSLKNVQNGQGL+V
Sbjct: 483 SSKTLKTFPLYMYSNTIDQGNGSYRSIANLTLGFNEKKTDGLGSFISSLKNVQNGQGLMV 542

Query: 542 VKGHLVTSGLGSTQQDYRYHDDVQCYSRNISSSNYTILHDNVKNTCGEKQNSPESLRFRR 601
           VKG+LVTSGLGSTQQDYRY+DDVQCYSR+ISSSNYTILHDNVKN CGEKQNS ESLRFRR
Sbjct: 543 VKGNLVTSGLGSTQQDYRYYDDVQCYSRSISSSNYTILHDNVKNMCGEKQNSSESLRFRR 602

Query: 602 WPIPARRAFLDSNLFVNNGRV 622
           WPIPARRA LDSNLFVNNG V
Sbjct: 603 WPIPARRASLDSNLFVNNGHV 620

BLAST of CsGy4G021320 vs. NCBI nr
Match: XP_038899804.1 (peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A [Benincasa hispida])

HSP 1 Score: 1064 bits (2752), Expect = 0.0
Identity = 530/616 (86.04%), Postives = 557/616 (90.42%), Query Frame = 0

Query: 7   LLISLLLLLLLLLPFHSNANLHKTRLRRSDLFSEPDVTTTADHSTPPTVFFEVSKPIVTP 66
           LL+ L+ L+ LLLPFHSNANLHK RLR SDL S+  +TTTAD++TPPTVFFEV+KPI TP
Sbjct: 14  LLVLLISLIFLLLPFHSNANLHKIRLRHSDLLSDSGLTTTADYTTPPTVFFEVTKPIETP 73

Query: 67  KSKPCSLLLLHHDFAFTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFDRIFGV 126
           K+KPCSLL+L HDFAFTYGK PVLADYTPPSHCSFTKFSKIVLEW ATC+GRQFDRIFGV
Sbjct: 74  KAKPCSLLVLQHDFAFTYGKSPVLADYTPPSHCSFTKFSKIVLEWTATCQGRQFDRIFGV 133

Query: 127 WLGGVEILRSCTAEPRATGIVWTVQKDITRYSSLLSKNQTLAVYLGNLVDKTYTGIYNVK 186
           WLGGVEILRSCTAEPRATGIVWTVQKDITRY SLLSKNQTLAVYLGNLVDKTYTGIY+VK
Sbjct: 134 WLGGVEILRSCTAEPRATGIVWTVQKDITRYDSLLSKNQTLAVYLGNLVDKTYTGIYHVK 193

Query: 187 IRIHFYPEEEGFGGNGVYSQKLGSAYDSDSRADLILPISRNFPLNDGLWFEVQNSTDVEF 246
           I +HFYPE +     GV S+KLGSAYDSDSRADLILPISRN PLNDGLWFEVQNSTDV+ 
Sbjct: 194 IHVHFYPEAD----TGVSSEKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNSTDVQS 253

Query: 247 KEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVLVDLDGE 306
           KEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVLV LDGE
Sbjct: 254 KEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVLVSLDGE 313

Query: 307 IVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKVHNFGFRVTH 366
           IVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLG+LLDGKVHN GF VTH
Sbjct: 314 IVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGHLLDGKVHNCGFSVTH 373

Query: 367 ALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGPDGTFLTKVTRSVSSA 426
           ALNVW+INANLHLWLDDNS KTE KLL+HIVS P +S DLNFTGPDGTFLTKVTRSVSS 
Sbjct: 374 ALNVWYINANLHLWLDDNSAKTEGKLLDHIVSPPFISVDLNFTGPDGTFLTKVTRSVSST 433

Query: 427 GWVKSSFGIITTLSNQDLSYSNSMTMGNNGSSQTVNQEIQFNTSVYAKKESSHVYSVKSL 486
           GWVKSSFGII T SNQDLSYSNSM MGNNGSSQTV QEIQFN SVYAK+ESSHVYS KSL
Sbjct: 434 GWVKSSFGIINTQSNQDLSYSNSMVMGNNGSSQTVTQEIQFNGSVYAKQESSHVYSFKSL 493

Query: 487 KTFPLYMYSNTKDEENGSYQSIANLTLGFNEKKTDGLGKVISSLKNVQNGQGLIVVKGHL 546
           KTFPLYMYSNT DE NG+YQSIANLTLGFNEKKTDG G   SSLKN+QNGQGL+VVKGHL
Sbjct: 494 KTFPLYMYSNTIDEGNGTYQSIANLTLGFNEKKTDGFGSFTSSLKNLQNGQGLMVVKGHL 553

Query: 547 VTSGLGSTQQDYRYHDDVQCYSRNISSSNYTILHDNVKNTCGEKQNSPESLRFRRWPIPA 606
           VTSGLGSTQQDYRYHDD+QCYSRNISSSNYTIL+DNVK+ CG K      L FR   IPA
Sbjct: 554 VTSGLGSTQQDYRYHDDIQCYSRNISSSNYTILYDNVKDFCGRKTRILRKLGFRGGSIPA 613

Query: 607 RRAFLDSNLFVNNGRV 622
           RRAFLDSNL VNN  V
Sbjct: 614 RRAFLDSNLHVNNSHV 625

BLAST of CsGy4G021320 vs. NCBI nr
Match: XP_022140996.1 (peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A [Momordica charantia])

HSP 1 Score: 1025 bits (2649), Expect = 0.0
Identity = 505/610 (82.79%), Postives = 545/610 (89.34%), Query Frame = 0

Query: 14  LLLLLLPFHSNANLHKTRLRRSDLFSEPDVTTTADHSTPPTVFFEVSKPIVTPKSKPCSL 73
           LLLLLLPF+S+ANLHKTR RR DL SE   T    H+ PPT+FFEV+KPI TPK+KPCS+
Sbjct: 13  LLLLLLPFYSDANLHKTRFRRPDLLSELTATADLGHTPPPTLFFEVTKPIETPKAKPCSV 72

Query: 74  LLLHHDFAFTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFDRIFGVWLGGVEI 133
           L L HDF +TYGK PVLADYTPPSHCSFTKFSKIVLEW ATCRGRQFDRIFGVWLGGVEI
Sbjct: 73  LALQHDFGYTYGKSPVLADYTPPSHCSFTKFSKIVLEWMATCRGRQFDRIFGVWLGGVEI 132

Query: 134 LRSCTAEPRATGIVWTVQKDITRYSSLLSKNQTLAVYLGNLVDKTYTGIYNVKIRIHFYP 193
           LRSCTAEPRATGIVWTV+KDITRYSSLLSKNQTLAVYLGNLVDKTYTGIY+V+I IHFYP
Sbjct: 133 LRSCTAEPRATGIVWTVRKDITRYSSLLSKNQTLAVYLGNLVDKTYTGIYHVRIHIHFYP 192

Query: 194 EEEGFGGNGV-YSQKLGSAYDSDSRADLILPISRNFPLNDGLWFEVQNSTDVEFKEFEIP 253
           EE+ FG NG  + +K  S YDS   ADLI+PISR+ PLNDGLWFEVQ+STDV+ KEFEIP
Sbjct: 193 EEKSFGDNGGRFLEKSASGYDSG--ADLIVPISRSLPLNDGLWFEVQSSTDVQVKEFEIP 252

Query: 254 QNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVLVDLDGEIVGAVW 313
           QNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVLV LDG+IVGAVW
Sbjct: 253 QNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVLVSLDGQIVGAVW 312

Query: 314 PFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKVHNFGFRVTHALNVWF 373
           PFTV+YTGGVNPLLWRPI+GIGSFNLPSYDIE+TPFLGNLLDGK HNFGFRVTHALNVW+
Sbjct: 313 PFTVVYTGGVNPLLWRPITGIGSFNLPSYDIEITPFLGNLLDGKAHNFGFRVTHALNVWY 372

Query: 374 INANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGPDGTFLTKVTRSVSSAGWVKSS 433
           INANLHLWLDDNS KTE KLLNHIVS    S++ NFTGPDGTF TKVTRSVSS GWVKSS
Sbjct: 373 INANLHLWLDDNSAKTEGKLLNHIVSPLVNSEESNFTGPDGTFSTKVTRSVSSIGWVKSS 432

Query: 434 FGIITTLSNQDLSYSNSMTMGNNGSSQTVNQEIQFNTSVYAKKESSHVYSVKSLKTFPLY 493
            GIITT SNQD SYSNSM +G+NGSSQ VNQ+IQFN+SVYAK++SSHVYS KSLKTFPLY
Sbjct: 433 RGIITTQSNQDFSYSNSMEIGSNGSSQVVNQKIQFNSSVYAKQQSSHVYSFKSLKTFPLY 492

Query: 494 MYSNTKDEENGSYQSIANLTLGFNEKKTDGLGKVISSLKNVQNGQGLIVVKGHLVTSGLG 553
           +YS+TKDE NG+YQS+ANLTLGFNEKKTDG G  ISSLKN+QNGQGL+VVK HLVT GLG
Sbjct: 493 LYSDTKDEGNGTYQSLANLTLGFNEKKTDGFGSFISSLKNLQNGQGLMVVKDHLVTRGLG 552

Query: 554 STQQDYRYHDDVQCYSRNISSSNYTILHDNVKNTCGEKQNSPESLRFRRWPIPARRAFLD 613
           STQQDYRYHDD  CY RNISSSNYTIL DNVKNTCG+K  SP SLRF RWPIPARRAFLD
Sbjct: 553 STQQDYRYHDDKLCYFRNISSSNYTILFDNVKNTCGKKPESPASLRFGRWPIPARRAFLD 612

Query: 614 SNLFVNNGRV 622
           S+L VN+  V
Sbjct: 613 SSLQVNSVHV 620

BLAST of CsGy4G021320 vs. NCBI nr
Match: KAG6607915.1 (hypothetical protein SDJN03_01257, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037442.1 hypothetical protein SDJN02_01069, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1001 bits (2589), Expect = 0.0
Identity = 493/609 (80.95%), Postives = 544/609 (89.33%), Query Frame = 0

Query: 3   SSFLLLISLLLLLLLLLPFHSNANLHKTRLRRSDLFSEPDVTTTADHSTPPTVFFEVSKP 62
           SS LLLISLL +LL   PFHSNA+LH+T LRR DLFSE ++  T +H+TPPT+FFEV+KP
Sbjct: 6   SSPLLLISLLFILL---PFHSNADLHRTSLRRPDLFSESELAATGNHTTPPTLFFEVTKP 65

Query: 63  IVTPKSKPCSLLLLHHDFAFTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFDR 122
           I TP+++PCSLLLL HDF +TYGK PVLADY+PPSHCSF++FS+IVLEWKATCRGRQFDR
Sbjct: 66  IETPEAQPCSLLLLQHDFGYTYGKSPVLADYSPPSHCSFSRFSRIVLEWKATCRGRQFDR 125

Query: 123 IFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYSSLLSKNQTLAVYLGNLVDKTYTGI 182
           IFGVWLGGVEILRSCTAEP+ATGIVWTV+KDITRY SLLSKNQTLAVYLGNL+DKTYTGI
Sbjct: 126 IFGVWLGGVEILRSCTAEPKATGIVWTVKKDITRYYSLLSKNQTLAVYLGNLIDKTYTGI 185

Query: 183 YNVKIRIHFYPEEEGFGGNGVYSQKLGSAYDSDSRADLILPISRNFPLNDGLWFEVQNST 242
           Y+VKI IHFYPEE+ F     YS+KL + YDS  RADLILPISR+ PLNDGLWFEVQNST
Sbjct: 186 YHVKIHIHFYPEEKRF----QYSEKLAAGYDS--RADLILPISRSLPLNDGLWFEVQNST 245

Query: 243 DVEFKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVLVD 302
           D++ KEFEIPQN YRAVLEVY+SFHENDEFWYSNLPNDYIIANNLT TPGNGPFREVLV+
Sbjct: 246 DIQVKEFEIPQNSYRAVLEVYLSFHENDEFWYSNLPNDYIIANNLTQTPGNGPFREVLVN 305

Query: 303 LDGEIVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKVHNFGF 362
           LD EIVGA+WPFTVIYTGGVNPLLWRPI+GIGSFNLPSYDIELTPFLG+LLDGK H+FGF
Sbjct: 306 LDDEIVGAIWPFTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGKSHHFGF 365

Query: 363 RVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGPDGTFLTKVTRS 422
            VTHALNVW+INANLHLWLDD S KTE KLLNHI+S  S S+DLNFTGPDGTFLTK +RS
Sbjct: 366 SVTHALNVWYINANLHLWLDDKSTKTEGKLLNHIISPLSNSEDLNFTGPDGTFLTKASRS 425

Query: 423 VSSAGWVKSSFGIITTLSNQDLSYSNSMTMGNNGSSQTVNQEIQFNTSVYAKKESSHVYS 482
           VSS+GWVKSS GIITT SNQD SYSNSM MGN+G+SQ VNQEIQFN+SVYAK+ES HVYS
Sbjct: 426 VSSSGWVKSSHGIITTQSNQDFSYSNSMVMGNDGNSQIVNQEIQFNSSVYAKEESDHVYS 485

Query: 483 VKSLKTFPLYMYSNTKDEENGSYQSIANLTLGFNEKKTDGLGKVISSLKNVQNGQGLIVV 542
            KSLKTFPLYMYSNT DE NGS+QSIANLTLGFNEKK DGLG  ISSLKNVQNGQG++ V
Sbjct: 486 FKSLKTFPLYMYSNTIDEGNGSFQSIANLTLGFNEKKADGLGSFISSLKNVQNGQGMMTV 545

Query: 543 KGHLVTSGLGSTQQDYRYHDDVQCYSRNISSSNYTILHDNVKNTCGEKQNSPESLRFRRW 602
           KGHLVT GLGSTQQDYRYHDD  CYSRNISSSNYTIL+D+V + C +K  SPESLRFR+W
Sbjct: 546 KGHLVTRGLGSTQQDYRYHDDKLCYSRNISSSNYTILYDDVNSLCDKKPESPESLRFRKW 605

Query: 603 PIPARRAFL 611
           P  +RR FL
Sbjct: 606 PFLSRRVFL 605

BLAST of CsGy4G021320 vs. ExPASy TrEMBL
Match: A0A0A0L222 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G627190 PE=4 SV=1)

HSP 1 Score: 1250 bits (3234), Expect = 0.0
Identity = 620/622 (99.68%), Postives = 620/622 (99.68%), Query Frame = 0

Query: 1   MASSFLLLISLLLLLLLLLPFHSNANLHKTRLRRSDLFSEPDVTTTADHSTPPTVFFEVS 60
           MASSFLLLISLLLLLLLLLPFHSNANLHKTRLRRSDLFSEPDVTTTADHSTPPTVFFEVS
Sbjct: 1   MASSFLLLISLLLLLLLLLPFHSNANLHKTRLRRSDLFSEPDVTTTADHSTPPTVFFEVS 60

Query: 61  KPIVTPKSKPCSLLLLHHDFAFTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQF 120
           KPIVTPKSKPCSLLLLHHDFAFTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQF
Sbjct: 61  KPIVTPKSKPCSLLLLHHDFAFTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQF 120

Query: 121 DRIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYSSLLSKNQTLAVYLGNLVDKTYT 180
           DRIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYSSLLSKNQTLAVYLGNLVDKTYT
Sbjct: 121 DRIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYSSLLSKNQTLAVYLGNLVDKTYT 180

Query: 181 GIYNVKIRIHFYPEEEGFGGNGVYSQKLGSAYDSDSRADLILPISRNFPLNDGLWFEVQN 240
           GIYNVKIRIHFYPEEEGFGGNGVYSQKLGSAYDSDSRADLILPISRN PLNDGLWFEVQN
Sbjct: 181 GIYNVKIRIHFYPEEEGFGGNGVYSQKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQN 240

Query: 241 STDVEFKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVL 300
           STDVEFKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVL
Sbjct: 241 STDVEFKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVL 300

Query: 301 VDLDGEIVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKVHNF 360
           VDLDGEIVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKVHNF
Sbjct: 301 VDLDGEIVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKVHNF 360

Query: 361 GFRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGPDGTFLTKVT 420
           GFRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGPDGTFLTKVT
Sbjct: 361 GFRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGPDGTFLTKVT 420

Query: 421 RSVSSAGWVKSSFGIITTLSNQDLSYSNSMTMGNNGSSQTVNQEIQFNTSVYAKKESSHV 480
           RSVSS GWVKSSFGIITTLSNQDLSYSNSMTMGNNGSSQTVNQEIQFNTSVYAKKESSHV
Sbjct: 421 RSVSSTGWVKSSFGIITTLSNQDLSYSNSMTMGNNGSSQTVNQEIQFNTSVYAKKESSHV 480

Query: 481 YSVKSLKTFPLYMYSNTKDEENGSYQSIANLTLGFNEKKTDGLGKVISSLKNVQNGQGLI 540
           YSVKSLKTFPLYMYSNTKDEENGSYQSIANLTLGFNEKKTDGLGKVISSLKNVQNGQGLI
Sbjct: 481 YSVKSLKTFPLYMYSNTKDEENGSYQSIANLTLGFNEKKTDGLGKVISSLKNVQNGQGLI 540

Query: 541 VVKGHLVTSGLGSTQQDYRYHDDVQCYSRNISSSNYTILHDNVKNTCGEKQNSPESLRFR 600
           VVKGHLVTSGLGSTQQDYRYHDDVQCYSRNISSSNYTILHDNVKNTCGEKQNSPESLRFR
Sbjct: 541 VVKGHLVTSGLGSTQQDYRYHDDVQCYSRNISSSNYTILHDNVKNTCGEKQNSPESLRFR 600

Query: 601 RWPIPARRAFLDSNLFVNNGRV 622
           RWPIPARRAFLDSNLFVNNGRV
Sbjct: 601 RWPIPARRAFLDSNLFVNNGRV 622

BLAST of CsGy4G021320 vs. ExPASy TrEMBL
Match: A0A5D3DX64 (Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G001000 PE=4 SV=1)

HSP 1 Score: 1158 bits (2995), Expect = 0.0
Identity = 572/621 (92.11%), Postives = 597/621 (96.14%), Query Frame = 0

Query: 2   ASSFLLLISLLLLLLLLLPFHSNANLHKTRLRRSDLFSEPDVTTTADHSTPPTVFFEVSK 61
           +SSFLLLISLLLLLL   PFHS+A+LHKTRLR+SDLFSE  V+TTADHSTPPTVFFEV+K
Sbjct: 3   SSSFLLLISLLLLLL---PFHSDADLHKTRLRQSDLFSESHVSTTADHSTPPTVFFEVTK 62

Query: 62  PIVTPKSKPCSLLLLHHDFAFTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFD 121
           PIVTPK+KPCSLL+LHHDFA+TYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFD
Sbjct: 63  PIVTPKAKPCSLLVLHHDFAYTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFD 122

Query: 122 RIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYSSLLSKNQTLAVYLGNLVDKTYTG 181
           RIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRY+SLLSKNQTLAVYLGNLVDKTYTG
Sbjct: 123 RIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLLSKNQTLAVYLGNLVDKTYTG 182

Query: 182 IYNVKIRIHFYPEEEGFGGNGVYSQKLGSAYDSDSRADLILPISRNFPLNDGLWFEVQNS 241
           IYNVKI IHFYPEEEGFGGNGVYS+KLGSAYDSDSRADLILPISRN PLNDGLWFEVQNS
Sbjct: 183 IYNVKIHIHFYPEEEGFGGNGVYSEKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNS 242

Query: 242 TDVEFKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVLV 301
           TDV+ KEFEIPQNVYRA+LEVYVSFHENDEFWYSNLPNDYI+ANNLTDTPGNGPFREVLV
Sbjct: 243 TDVQLKEFEIPQNVYRALLEVYVSFHENDEFWYSNLPNDYIVANNLTDTPGNGPFREVLV 302

Query: 302 DLDGEIVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKVHNFG 361
           DLDG+IV AVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGK H+FG
Sbjct: 303 DLDGDIVAAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKGHSFG 362

Query: 362 FRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGPDGTFLTKVTR 421
           FRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTG DGTFLTKVTR
Sbjct: 363 FRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGADGTFLTKVTR 422

Query: 422 SVSSAGWVKSSFGIITTLSNQDLSYSNSMTMGNNGSSQTVNQEIQFNTSVYAKKESSHVY 481
           SVSSAGWVKSSFG ITTLSNQDLSYSN M MGNNGSSQTVNQEI FN+SVYAK+ESSHVY
Sbjct: 423 SVSSAGWVKSSFGTITTLSNQDLSYSNLMMMGNNGSSQTVNQEIHFNSSVYAKQESSHVY 482

Query: 482 SVKSLKTFPLYMYSNTKDEENGSYQSIANLTLGFNEKKTDGLGKVISSLKNVQNGQGLIV 541
           S K+LKTFPLYMYSNT D+ NGSY+SIANLTLGFNEKKTDGLG  ISSLKNVQNGQGL+V
Sbjct: 483 SSKTLKTFPLYMYSNTIDQGNGSYRSIANLTLGFNEKKTDGLGSFISSLKNVQNGQGLMV 542

Query: 542 VKGHLVTSGLGSTQQDYRYHDDVQCYSRNISSSNYTILHDNVKNTCGEKQNSPESLRFRR 601
           VKG+LVTSGLGSTQQDYRY+DDVQCYSR+ISSSNYTILHDNVKN CGEKQNS ESLRFRR
Sbjct: 543 VKGNLVTSGLGSTQQDYRYYDDVQCYSRSISSSNYTILHDNVKNMCGEKQNSSESLRFRR 602

Query: 602 WPIPARRAFLDSNLFVNNGRV 622
           WPIPARRA LDSNLFVNNG V
Sbjct: 603 WPIPARRASLDSNLFVNNGHV 620

BLAST of CsGy4G021320 vs. ExPASy TrEMBL
Match: A0A1S3CJY5 (peptide-N4-(N-acetyl-beta- glucosaminyl)asparagine amidase A OS=Cucumis melo OX=3656 GN=LOC103501812 PE=4 SV=1)

HSP 1 Score: 1158 bits (2995), Expect = 0.0
Identity = 572/621 (92.11%), Postives = 597/621 (96.14%), Query Frame = 0

Query: 2   ASSFLLLISLLLLLLLLLPFHSNANLHKTRLRRSDLFSEPDVTTTADHSTPPTVFFEVSK 61
           +SSFLLLISLLLLLL   PFHS+A+LHKTRLR+SDLFSE  V+TTADHSTPPTVFFEV+K
Sbjct: 3   SSSFLLLISLLLLLL---PFHSDADLHKTRLRQSDLFSESHVSTTADHSTPPTVFFEVTK 62

Query: 62  PIVTPKSKPCSLLLLHHDFAFTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFD 121
           PIVTPK+KPCSLL+LHHDFA+TYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFD
Sbjct: 63  PIVTPKAKPCSLLVLHHDFAYTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFD 122

Query: 122 RIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYSSLLSKNQTLAVYLGNLVDKTYTG 181
           RIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRY+SLLSKNQTLAVYLGNLVDKTYTG
Sbjct: 123 RIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYNSLLSKNQTLAVYLGNLVDKTYTG 182

Query: 182 IYNVKIRIHFYPEEEGFGGNGVYSQKLGSAYDSDSRADLILPISRNFPLNDGLWFEVQNS 241
           IYNVKI IHFYPEEEGFGGNGVYS+KLGSAYDSDSRADLILPISRN PLNDGLWFEVQNS
Sbjct: 183 IYNVKIHIHFYPEEEGFGGNGVYSEKLGSAYDSDSRADLILPISRNLPLNDGLWFEVQNS 242

Query: 242 TDVEFKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVLV 301
           TDV+ KEFEIPQNVYRA+LEVYVSFHENDEFWYSNLPNDYI+ANNLTDTPGNGPFREVLV
Sbjct: 243 TDVQLKEFEIPQNVYRALLEVYVSFHENDEFWYSNLPNDYIVANNLTDTPGNGPFREVLV 302

Query: 302 DLDGEIVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKVHNFG 361
           DLDG+IV AVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGK H+FG
Sbjct: 303 DLDGDIVAAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKGHSFG 362

Query: 362 FRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGPDGTFLTKVTR 421
           FRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTG DGTFLTKVTR
Sbjct: 363 FRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGADGTFLTKVTR 422

Query: 422 SVSSAGWVKSSFGIITTLSNQDLSYSNSMTMGNNGSSQTVNQEIQFNTSVYAKKESSHVY 481
           SVSSAGWVKSSFG ITTLSNQDLSYSN M MGNNGSSQTVNQEI FN+SVYAK+ESSHVY
Sbjct: 423 SVSSAGWVKSSFGTITTLSNQDLSYSNLMMMGNNGSSQTVNQEIHFNSSVYAKQESSHVY 482

Query: 482 SVKSLKTFPLYMYSNTKDEENGSYQSIANLTLGFNEKKTDGLGKVISSLKNVQNGQGLIV 541
           S K+LKTFPLYMYSNT D+ NGSY+SIANLTLGFNEKKTDGLG  ISSLKNVQNGQGL+V
Sbjct: 483 SSKTLKTFPLYMYSNTIDQGNGSYRSIANLTLGFNEKKTDGLGSFISSLKNVQNGQGLMV 542

Query: 542 VKGHLVTSGLGSTQQDYRYHDDVQCYSRNISSSNYTILHDNVKNTCGEKQNSPESLRFRR 601
           VKG+LVTSGLGSTQQDYRY+DDVQCYSR+ISSSNYTILHDNVKN CGEKQNS ESLRFRR
Sbjct: 543 VKGNLVTSGLGSTQQDYRYYDDVQCYSRSISSSNYTILHDNVKNMCGEKQNSSESLRFRR 602

Query: 602 WPIPARRAFLDSNLFVNNGRV 622
           WPIPARRA LDSNLFVNNG V
Sbjct: 603 WPIPARRASLDSNLFVNNGHV 620

BLAST of CsGy4G021320 vs. ExPASy TrEMBL
Match: A0A6J1CHN7 (peptide-N4-(N-acetyl-beta- glucosaminyl)asparagine amidase A OS=Momordica charantia OX=3673 GN=LOC111011511 PE=4 SV=1)

HSP 1 Score: 1025 bits (2649), Expect = 0.0
Identity = 505/610 (82.79%), Postives = 545/610 (89.34%), Query Frame = 0

Query: 14  LLLLLLPFHSNANLHKTRLRRSDLFSEPDVTTTADHSTPPTVFFEVSKPIVTPKSKPCSL 73
           LLLLLLPF+S+ANLHKTR RR DL SE   T    H+ PPT+FFEV+KPI TPK+KPCS+
Sbjct: 13  LLLLLLPFYSDANLHKTRFRRPDLLSELTATADLGHTPPPTLFFEVTKPIETPKAKPCSV 72

Query: 74  LLLHHDFAFTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFDRIFGVWLGGVEI 133
           L L HDF +TYGK PVLADYTPPSHCSFTKFSKIVLEW ATCRGRQFDRIFGVWLGGVEI
Sbjct: 73  LALQHDFGYTYGKSPVLADYTPPSHCSFTKFSKIVLEWMATCRGRQFDRIFGVWLGGVEI 132

Query: 134 LRSCTAEPRATGIVWTVQKDITRYSSLLSKNQTLAVYLGNLVDKTYTGIYNVKIRIHFYP 193
           LRSCTAEPRATGIVWTV+KDITRYSSLLSKNQTLAVYLGNLVDKTYTGIY+V+I IHFYP
Sbjct: 133 LRSCTAEPRATGIVWTVRKDITRYSSLLSKNQTLAVYLGNLVDKTYTGIYHVRIHIHFYP 192

Query: 194 EEEGFGGNGV-YSQKLGSAYDSDSRADLILPISRNFPLNDGLWFEVQNSTDVEFKEFEIP 253
           EE+ FG NG  + +K  S YDS   ADLI+PISR+ PLNDGLWFEVQ+STDV+ KEFEIP
Sbjct: 193 EEKSFGDNGGRFLEKSASGYDSG--ADLIVPISRSLPLNDGLWFEVQSSTDVQVKEFEIP 252

Query: 254 QNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVLVDLDGEIVGAVW 313
           QNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVLV LDG+IVGAVW
Sbjct: 253 QNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVLVSLDGQIVGAVW 312

Query: 314 PFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKVHNFGFRVTHALNVWF 373
           PFTV+YTGGVNPLLWRPI+GIGSFNLPSYDIE+TPFLGNLLDGK HNFGFRVTHALNVW+
Sbjct: 313 PFTVVYTGGVNPLLWRPITGIGSFNLPSYDIEITPFLGNLLDGKAHNFGFRVTHALNVWY 372

Query: 374 INANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGPDGTFLTKVTRSVSSAGWVKSS 433
           INANLHLWLDDNS KTE KLLNHIVS    S++ NFTGPDGTF TKVTRSVSS GWVKSS
Sbjct: 373 INANLHLWLDDNSAKTEGKLLNHIVSPLVNSEESNFTGPDGTFSTKVTRSVSSIGWVKSS 432

Query: 434 FGIITTLSNQDLSYSNSMTMGNNGSSQTVNQEIQFNTSVYAKKESSHVYSVKSLKTFPLY 493
            GIITT SNQD SYSNSM +G+NGSSQ VNQ+IQFN+SVYAK++SSHVYS KSLKTFPLY
Sbjct: 433 RGIITTQSNQDFSYSNSMEIGSNGSSQVVNQKIQFNSSVYAKQQSSHVYSFKSLKTFPLY 492

Query: 494 MYSNTKDEENGSYQSIANLTLGFNEKKTDGLGKVISSLKNVQNGQGLIVVKGHLVTSGLG 553
           +YS+TKDE NG+YQS+ANLTLGFNEKKTDG G  ISSLKN+QNGQGL+VVK HLVT GLG
Sbjct: 493 LYSDTKDEGNGTYQSLANLTLGFNEKKTDGFGSFISSLKNLQNGQGLMVVKDHLVTRGLG 552

Query: 554 STQQDYRYHDDVQCYSRNISSSNYTILHDNVKNTCGEKQNSPESLRFRRWPIPARRAFLD 613
           STQQDYRYHDD  CY RNISSSNYTIL DNVKNTCG+K  SP SLRF RWPIPARRAFLD
Sbjct: 553 STQQDYRYHDDKLCYFRNISSSNYTILFDNVKNTCGKKPESPASLRFGRWPIPARRAFLD 612

Query: 614 SNLFVNNGRV 622
           S+L VN+  V
Sbjct: 613 SSLQVNSVHV 620

BLAST of CsGy4G021320 vs. ExPASy TrEMBL
Match: A0A6J1FLZ6 (peptide-N4-(N-acetyl-beta- glucosaminyl)asparagine amidase A-like OS=Cucurbita moschata OX=3662 GN=LOC111446428 PE=4 SV=1)

HSP 1 Score: 1000 bits (2585), Expect = 0.0
Identity = 486/598 (81.27%), Postives = 537/598 (89.80%), Query Frame = 0

Query: 14  LLLLLLPFHSNANLHKTRLRRSDLFSEPDVTTTADHSTPPTVFFEVSKPIVTPKSKPCSL 73
           LL +LLPFHSNA+LH+T LRR DLFSE ++  T +H+TPPT+FFEV+KPI TP+++PCSL
Sbjct: 13  LLFILLPFHSNADLHRTSLRRPDLFSESELAATGNHTTPPTLFFEVTKPIETPEAQPCSL 72

Query: 74  LLLHHDFAFTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFDRIFGVWLGGVEI 133
           LLL HDF +TYGK PVLADY+PPSHCSF+KFSKIVLEWKATCRGRQFDRIFGVWLGGVEI
Sbjct: 73  LLLQHDFGYTYGKSPVLADYSPPSHCSFSKFSKIVLEWKATCRGRQFDRIFGVWLGGVEI 132

Query: 134 LRSCTAEPRATGIVWTVQKDITRYSSLLSKNQTLAVYLGNLVDKTYTGIYNVKIRIHFYP 193
           LRSCTAEP+ATGIVWTV+KDITRY SLLSKNQTLAVYLGNL+DKTYTGIY+VKI IHFYP
Sbjct: 133 LRSCTAEPKATGIVWTVKKDITRYYSLLSKNQTLAVYLGNLIDKTYTGIYHVKIHIHFYP 192

Query: 194 EEEGFGGNGVYSQKLGSAYDSDSRADLILPISRNFPLNDGLWFEVQNSTDVEFKEFEIPQ 253
           EE+ F     YS+KL + YDS  RADLILPISR+ PLNDGLWFEVQNSTD++ KEFEIPQ
Sbjct: 193 EEKRF----QYSEKLAAGYDS--RADLILPISRSLPLNDGLWFEVQNSTDIQVKEFEIPQ 252

Query: 254 NVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVLVDLDGEIVGAVWP 313
           N YRAVLEVY+SFHENDEFWYSNLPNDYI+ANNL+ TPGNGPFREVLV+LD EIVGA+WP
Sbjct: 253 NSYRAVLEVYLSFHENDEFWYSNLPNDYILANNLSQTPGNGPFREVLVNLDDEIVGAIWP 312

Query: 314 FTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKVHNFGFRVTHALNVWFI 373
           FTVIYTGGVNPLLWRPI+GIGSFNLPSYDIELTPFLG+LLDGK H+FGF VTHALNVW+I
Sbjct: 313 FTVIYTGGVNPLLWRPITGIGSFNLPSYDIELTPFLGHLLDGKSHHFGFSVTHALNVWYI 372

Query: 374 NANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGPDGTFLTKVTRSVSSAGWVKSSF 433
           NANLHLWLDD S KTE KLLNHI+S  S S+DLNFTGPDGTFLTK +RSVSS+GWVKSS 
Sbjct: 373 NANLHLWLDDKSTKTEGKLLNHIISPLSNSEDLNFTGPDGTFLTKASRSVSSSGWVKSSH 432

Query: 434 GIITTLSNQDLSYSNSMTMGNNGSSQTVNQEIQFNTSVYAKKESSHVYSVKSLKTFPLYM 493
           GIITT SNQD SYSNSM MGN+G+SQ VNQEIQFN+SVYAK+ES HVYS KSLKTFPLYM
Sbjct: 433 GIITTQSNQDFSYSNSMVMGNDGNSQIVNQEIQFNSSVYAKEESGHVYSFKSLKTFPLYM 492

Query: 494 YSNTKDEENGSYQSIANLTLGFNEKKTDGLGKVISSLKNVQNGQGLIVVKGHLVTSGLGS 553
           YSNT DE NGS+QSIANLTLGFNEKK DGLG  ISSLKNVQNGQG++ VKGHLVT GLGS
Sbjct: 493 YSNTIDEGNGSFQSIANLTLGFNEKKADGLGSFISSLKNVQNGQGMMTVKGHLVTRGLGS 552

Query: 554 TQQDYRYHDDVQCYSRNISSSNYTILHDNVKNTCGEKQNSPESLRFRRWPIPARRAFL 611
           TQQDYRYHDD  CYSRNISSSNYTIL+D+V + C +K  SPESLRFR+WP  +RR FL
Sbjct: 553 TQQDYRYHDDKLCYSRNISSSNYTILYDDVNSLCDKKPESPESLRFRKWPFLSRRVFL 604

BLAST of CsGy4G021320 vs. TAIR 10
Match: AT3G14920.1 (Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A protein )

HSP 1 Score: 659.1 bits (1699), Expect = 3.6e-189
Identity = 333/581 (57.31%), Postives = 423/581 (72.81%), Query Frame = 0

Query: 25  ANLHKTRLRRSDLFSEPDVTTTADHSTP------PTVFFEVSKPIV---TPKSKPCSLLL 84
           ++LH+TR R    F  P  +     S P      PT +FEV KP V       +PCS  +
Sbjct: 24  SDLHETRSR----FKPPQFSPLFFSSLPQNVTKSPTRYFEVQKPPVPNLPTAQQPCSYQI 83

Query: 85  LHHDFAFTYGKPPVLADYTPPSHCSFTKFSKIVLEWKATCRGRQFDRIFGVWLGGVEILR 144
           LHHDF +TY KPPVL++YT PSHCS  +FSKIVLE+K+T +GRQFDRIFGVWL GVEILR
Sbjct: 84  LHHDFGYTYAKPPVLSNYTLPSHCSSREFSKIVLEFKSTSQGRQFDRIFGVWLDGVEILR 143

Query: 145 SCTAEPRATGIVWTVQKDITRYSSLLSKNQT--LAVYLGNLVDKTYTGIYNVKIRIHFYP 204
           SCTAEPR  GIVW+V+KD+T+Y SLL KN+T  L+VYLGNL+DKTYTG+Y+V +  HFY 
Sbjct: 144 SCTAEPRPNGIVWSVEKDVTKYHSLLVKNETQILSVYLGNLIDKTYTGVYHVDVIFHFYQ 203

Query: 205 EEEGFGGNGVYSQKLGSAYDSDSRADLILPISRNFPLNDGLWFEVQNSTDVEFKEFEIPQ 264
            E        Y         S S+AD+ILPISRN PLNDGLWFE+ NS D ++KEFEIP+
Sbjct: 204 SESNLQDVSGY---------SSSKADMILPISRNLPLNDGLWFEIVNSNDTKYKEFEIPR 263

Query: 265 NVYRAVLEVYVSFHENDEFWYSNLPNDYIIANNLTDTPGNGPFREVLVDLDGEIVGAVWP 324
           NVYRAVLEVYVSFHENDEFWY NLPNDY+ ANNL+   GNGPFREV+V LDG+I GAVWP
Sbjct: 264 NVYRAVLEVYVSFHENDEFWYGNLPNDYVTANNLS-VAGNGPFREVVVSLDGDIAGAVWP 323

Query: 325 FTVIYTGGVNPLLWRPISGIGSFNLPSYDIELTPFLGNLLDGKVHNFGFRVTHALNVWFI 384
           F V++TGG+NPLLWRPI+ IGSF+LPSYDIE+TPFLG+LLDGK H  GF VT+ALNVW+I
Sbjct: 324 FPVVFTGGINPLLWRPITAIGSFDLPSYDIEITPFLGSLLDGKTHKVGFSVTNALNVWYI 383

Query: 385 NANLHLWLDDNSVKTEAKLLNHIVSSPSMSQDLNFTGPDGTFLTKVTRSVSSAGWVKSSF 444
           +ANLHLWLD      E K+L+   SS  +S   +F G +G F TK  RS++S G VKSS 
Sbjct: 384 DANLHLWLDQEKEIVEGKVLDFSRSSLEISSVSDFKGLNGNFTTKAKRSITSVGLVKSSH 443

Query: 445 GIITTLSNQDLSYSNSMTMGNNGSSQTVNQEIQFNTSVYAKKESSHVYSVKSLKTFPLYM 504
           G I T +NQ+ SY N M +G +G+ Q ++Q IQ +  ++AK+ S  +Y+ KS+K+FP Y+
Sbjct: 444 GDIITNANQEFSYENKMVLGKDGNLQIIDQLIQADDRIHAKRASREIYAAKSIKSFPFYL 503

Query: 505 YSNTKDEENGSYQSIANLTLGFNEKKTD---GLGKVI-SSLKNVQNGQGLIVVKGHLVTS 564
            S+T +++N +Y ++AN+++ FNE++++   GL +   S L+N Q GQG++VVK +LV S
Sbjct: 504 DSDTLEQQNNTYLAVANVSMAFNEERSESDKGLMRTFKSKLENKQEGQGVMVVKNNLVVS 563

Query: 565 GLGSTQQDYRYHDDVQCYSRNISSSNYTILHDNVKNTCGEK 591
           G GSTQQ Y Y    QCY RNISS NYTIL+D V++ C +K
Sbjct: 564 GYGSTQQVYNYVGSDQCYFRNISSYNYTILYDKVESVCKKK 590

BLAST of CsGy4G021320 vs. TAIR 10
Match: AT5G05480.1 (Peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase A protein )

HSP 1 Score: 394.0 bits (1011), Expect = 2.2e-109
Identity = 222/547 (40.59%), Postives = 315/547 (57.59%), Query Frame = 0

Query: 53  PTVFFEVSKPIVTPKSKP-CSLLLLHHDFAFTYGKPPVLADYTPPSHCSFTKFSKIVLEW 112
           P  + E+ +P+ + +  P CS +L  H FA T  +PP    YTPPS C    +S +VL+ 
Sbjct: 46  PQEYEELRRPLPSDQLTPSCSHVLFRHSFANTINRPPFTTPYTPPSSCISPPWSYVVLDL 105

Query: 113 KATCRGRQFDRIFGVWLGGVEILRSCTAEPRATGIVWTVQKDITRYSSLLSKNQ-TLAVY 172
           +A   G Q+DRI G+WLGGVE+LR+ TAEP  +GI W V+KD++RYSSL  ++   + + 
Sbjct: 106 RAASSGDQYDRISGLWLGGVELLRTSTAEPSPSGIFWNVRKDVSRYSSLFMRSDLNVTMM 165

Query: 173 LGNLVDKTYTGIYNVKIRIHFYPEEEGFGGNGVYSQKLGSAYDSDSR----ADLILPISR 232
           L N+V+  YTGIY++ + + FY E      N   S +LG   DS  R    ADLI+P+  
Sbjct: 166 LENIVNDVYTGIYHINVTLIFY-EFNPIASNLKISNRLGFV-DSQKRDQTPADLIIPVCD 225

Query: 233 NFPLNDGLWFEVQNSTDVEFKEFEIPQNVYRAVLEVYVSFHENDEFWYSNLPNDYIIANN 292
               N G WF ++N  +   K  +IP N  + VLE+YVSFH NDEFWYSN PN YI  NN
Sbjct: 226 GG--NRGFWFMIENPRETYSKGIQIPSNTRQIVLELYVSFHGNDEFWYSNPPNSYIRTNN 285

Query: 293 LTDTPGNGPFREVLVDLDGEIVGAVWPFTVIYTGGVNPLLWRPISGIGSFNLPSYDIELT 352
           LT   GNG +REV V +DG  VG+  PF VI+TGG+NPL W P+  IG+FNLPSYD++LT
Sbjct: 286 LTTGRGNGAYREVFVKIDGRYVGSEVPFPVIFTGGINPLFWEPVVAIGAFNLPSYDMDLT 345

Query: 353 PFLGNLLDGKVHNFGFRVTHALNVWFINANLHLWLDDNSVKTEAKLLNHIVSSPS--MSQ 412
           PFLG LLDGK H F F V   ++ W ++ANLHLWLD  S   EA   + +  SPS  M +
Sbjct: 346 PFLGLLLDGKSHEFAFGVNDGISYWLVDANLHLWLDHGSSNVEAG--SRLYDSPSRHMMR 405

Query: 413 DLNFTGPDGTFLTKVTRSVSSAGWVKSSFGIITTLSNQDLSYSNSMTMGNNGSSQTVNQE 472
                  DG+F  +   S    GWV+SS G +TT+        + +    NG+ + V Q 
Sbjct: 406 QEQLEQLDGSFKVEAEVSSEYDGWVRSSKGNLTTMVKSMFKVDSLVRFEKNGTYKRVEQR 465

Query: 473 IQFNTSVYAKKESSH-VYSVKSLKTFPLYMYSNT---KDEENGSYQSIANLTLGFNEKKT 532
           ++    V    ES   V  V   +T+PL + ++T      +  +Y  + N++   NE  +
Sbjct: 466 VKTERIVEVTSESGKPVNRVVHQRTYPLTVITSTLRGLTNDKDTYMLVTNVSHALNETHS 525

Query: 533 DGLGKVISSLKNVQNGQGLIVVKGHLVTSGLGSTQQDYRYHDDVQCYSRNISSSNYTILH 588
           D  G+ +  + + Q+ +G + V+ H V +G  ST+Q   Y D+  CYSR I ++N  I  
Sbjct: 526 D--GEALIQVYSRQDSEGWMEVEDHNVLAGEASTKQSLSYIDEFSCYSRTIVAANGEIAQ 584