CsGy4G018790 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy4G018790
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionProteinase inhibitor I25, cystatin
LocationGy14Chr4: 24653626 .. 24656782 (-)
RNA-Seq ExpressionCsGy4G018790
SyntenyCsGy4G018790
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CATAGATGTGGATTAGATTCTATGTTATCAATGATGTGATGTTGAAAACAACAGTTATATAGAAATACCAAACTTGATCTCAATATTAATATCATATTGAGATCAATGAAGGGTTAGAAAATGTTTAGCCACCAAAGGTATTGTATAATGAGTGTTGAAGAAGCATATCACACTTCGAAACCTATCGAGGATATAAATGATGATCTGCAAGTGCAGTCAATCATAGGACAGTTGGCTGTTGAAGACCATAACAAGAAAACAGGAGACAATTTGGAGCTTGTGGATGTGGTGAATGGTTTGAGAAGTGGAATTTTTGTTCAACCTGGGAGTACTCATGAAGGAATATTATATCATCTTTTGGTGGAGGCTAAAACCATAGAAGGAATTAATTGGACATATGTGGCCAAGTTGTTAGAGCTTTACGTAGGCTGCCGAATAAGGTATGAATTCAAATCTTTTGAACCTCTTCTCCCATACTACAACCCCTAATCTAATATTTTACCCTCATCTCTCTACCCTACTTCTCACTCACATTCTAATTAAATAAATTGTATCTTTGTATATCAAAAATGTTCCTACCATATATATAAAAAAAAAGTAGTATTGAATATATTTATATTTTTAGTTTTGGGTAAATTACAAAAACCATATTTAAACATTTGAATTCAGCTTGTAATAATGATACGATTTATACACTTATCTAAGTTTGAGAATCCAATTTTAAAATTTGAATATATTTGAAAGTTTAATGTATTGTGCATATATGTATGTACGTGTCATTCCTAACTATTATTTGTGCGTATTCATGTGTGTGATCTCCGATTATCATTGTGCCTATAAGCACCGACAAATTTAGTATAGGCCTAGGAGGATCGAGTTCTCCCTCAACTTTATTATCTTTATAATATATGTATAAACAAAACTAATTAATGTTTAATATTTATTGATAGTTTAGTGGTAACTATGCTTGTCAATCTCTCTTTCGATCAGAGATCAAGTCCCTATAAGTGATGTGTATCAAAACCGAAAACACCTTAATTCCCACAACTCATAAGAACATTATAATCTCTTTTTTATTTTTTATTTTTTTCAATTTAACTCAAATTAATTCAATTTATTTTTATCATTTTTTTGGGTTCATTTGTATCAACCAATATCTACTGCTTTCTATCCTAAAACTAAATGATGACAAATAGTTAATCCAAGAAGGAAAATTGAGGAAGTTTGATATTAATAAAATTAGAGGCACTTGCATTCATCCAAAAATATAAAATTAAAATTAAGCTCATGATCCAACATTTTAAAATTTGGAAGAATGACAAATTAATTGCTACAATCCAATTGGTTTGATTATCAATAATATCGATTTGTCTGTTCTTTGACTACTGTCCGATTGTTATTTGATGTTCATTGTTATATGATTGTTAATGATACCGATTTATCATCTCCTTGTTATTTAATTTAATATTAATTTTTATTTAATTACAAATGATACTAATTGCTATATATGATTTTACTTTTTTTTTTATATAATTACTATGTAGGATTATATGATATAATCATCTCATCACCTTCCAGTTTTTTTTTTTTTTTTTTTTGTTAATTATTAAGTAGTTTCTATCACACCCTCTCCTGAAGCCTTTCTCTAACTCCTAACCTTGGCCCCAAAAGAGGGTGTGAGGGTTGACAAGGATTGCCATAGTTTGACAACACTTACTAACCATTTAACCTAACCGACCCAACCAAAAGTCACAAAATAACTTAAGAAAACAATATAAGCTATATGTTTTGCAAATAACTCGAACTACTAGCTACAACCTTAAAGTGTTATTAAAGCATTTTAGTGTAAATTTTGCTTTGATGTTAGTTTTAAACTGACATTATAGATGCCCTACAATGTTAATGTCTTCTGCATCGGTTTGTCACAAATTTTATTCAATAATTACCATATTGTCCAATCCATTTTTAACGACGATCAAATACTTAAAAATCGACATCAAATGTATCGGTATATATTGTTTACGCATGTGCAAATAAATAATCTTTTTTTTTTCAAGAAGCAATAATATCTCTTCTATTGTATACAACATAACTCTCAACAACACAAAATTTATCTCATTCACAACAAAGAAAGTGACAAATCGCTTAACTAAGTAACCTATTAGCAAATTACATTGATCAATATATACCCAAAGCTTTCAAAGTATAACGAACGAGGGAGTTGTCTAATATGAAGGATCATAGAGTTGTTGTCCCAAAAATATCTCAGTAAGGAAGATATGCTCACTTTAAGTACATAAAACAAATAATTAAAAATAAATTATTAATACCAAAAGAAATTTTTTTTTAAAAAAAGTACAAAACTTATATTTAATTTATACAAACTATTAAACCATGGAAGACTTAAAATCATATAACTTTAGACAACCAGCATATATTAAATTCCTAGAATTAAAACAATCTAACTTTCAAAAGTTATAAGGAGAATTATGGAGGGAGATATCATTTACAAAATAATATCTAAAATTAAGTACCCAATAATAAATCAAATATCAATTAAAAATGTTTCCATTATTTATCTTCATTTCTTTTTCTTTCAAATACCCAGTTGTCGCCCCCTCTTCACAGTGCTCTGTCCGGCCACAACTTCCATCATCGAACCCACCTCCATTCGACAAAGGTAGCCGCGAGCCAAAGCTTCCTCTTCGCTCCATCATGTCTTGATTCGTGAAGTCGAGTTGAGCCGCTTCGTCATCGAGCTCCCTGCGCAGGATTTTCAAAGTCAAACCAAGTGTCAGCCTTGAGTTGTCGTCCTTGACGCTTTTCGTTGCTCGTTGATGCTCTCTGTAGCTGCTACTCAAGTTTTGGTTAAGATGGTGATTTTGGTAGGTGTTCTTTTTTAGGCTTTATTGGGGATTTCTAATAGGAAACTTGCGGTGGGTAGCATTTTGAAAATATGACAATATAGCAAATATTGTTTTAGAATTTTGCAAATACGACAAAATTCTCACGTCTGAAAATATTTTTCATCTAAAATTTGTTATTATTTCCAAATTACCATTCAATAACTTATTTCTTCTTCTCTTTTTAATGCCTTAATCATCCCTTATTTCTTCTTCCATTTTTTAAGGCTTTGATCATCTTTTTTTTATACGGG

mRNA sequence

CATAGATGTGGATTAGATTCTATGTTATCAATGATGTGATGTTGAAAACAACAGTTATATAGAAATACCAAACTTGATCTCAATATTAATATCATATTGAGATCAATGAAGGGTTAGAAAATGTTTAGCCACCAAAGGTATTGTATAATGAGTGTTGAAGAAGCATATCACACTTCGAAACCTATCGAGGATATAAATGATGATCTGCAAGTGCAGTCAATCATAGGACAGTTGGCTGTTGAAGACCATAACAAGAAAACAGGAGACAATTTGGAGCTTGTGGATGTGGTGAATGGTTTGAGAAGTGGAATTTTTGTTCAACCTGGGAGTACTCATGAAGGAATATTATATCATCTTTTGGTGGAGGCTAAAACCATAGAAGGAATTAATTGGACATATGTGGCCAAGTTGTTAGAGCTTTACGTAGGCTGCCGAATAAGTTGTCGCCCCCTCTTCACAGTGCTCTGTCCGGCCACAACTTCCATCATCGAACCCACCTCCATTCGACAAAGGTAGCCGCGAGCCAAAGCTTCCTCTTCGCTCCATCATGTCTTGATTCGTGAAGTCGAGTTGAGCCGCTTCGTCATCGAGCTCCCTGCGCAGGATTTTCAAAGTCAAACCAAGTGTCAGCCTTGAGTTGTCGTCCTTGACGCTTTTCGTTGCTCGTTGATGCTCTCTGTAGCTGCTACTCAAGTTTTGGTTAAGATGGTGATTTTGGTAGGTGTTCTTTTTTAGGCTTTATTGGGGATTTCTAATAGGAAACTTGCGGTGGGTAGCATTTTGAAAATATGACAATATAGCAAATATTGTTTTAGAATTTTGCAAATACGACAAAATTCTCACGTCTGAAAATATTTTTCATCTAAAATTTGTTATTATTTCCAAATTACCATTCAATAACTTATTTCTTCTTCTCTTTTTAATGCCTTAATCATCCCTTATTTCTTCTTCCATTTTTTAAGGCTTTGATCATCTTTTTTTTATACGGG

Coding sequence (CDS)

ATGTTTAGCCACCAAAGGTATTGTATAATGAGTGTTGAAGAAGCATATCACACTTCGAAACCTATCGAGGATATAAATGATGATCTGCAAGTGCAGTCAATCATAGGACAGTTGGCTGTTGAAGACCATAACAAGAAAACAGGAGACAATTTGGAGCTTGTGGATGTGGTGAATGGTTTGAGAAGTGGAATTTTTGTTCAACCTGGGAGTACTCATGAAGGAATATTATATCATCTTTTGGTGGAGGCTAAAACCATAGAAGGAATTAATTGGACATATGTGGCCAAGTTGTTAGAGCTTTACGTAGGCTGCCGAATAAGTTGTCGCCCCCTCTTCACAGTGCTCTGTCCGGCCACAACTTCCATCATCGAACCCACCTCCATTCGACAAAGGTAG

Protein sequence

MFSHQRYCIMSVEEAYHTSKPIEDINDDLQVQSIIGQLAVEDHNKKTGDNLELVDVVNGLRSGIFVQPGSTHEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRISCRPLFTVLCPATTSIIEPTSIRQR*
Homology
BLAST of CsGy4G018790 vs. NCBI nr
Match: XP_031739698.1 (uncharacterized protein LOC116403239 [Cucumis sativus] >KAE8649718.1 hypothetical protein Csa_012480 [Cucumis sativus])

HSP 1 Score: 268 bits (686), Expect = 1.04e-90
Identity = 131/131 (100.00%), Postives = 131/131 (100.00%), Query Frame = 0

Query: 1   MFSHQRYCIMSVEEAYHTSKPIEDINDDLQVQSIIGQLAVEDHNKKTGDNLELVDVVNGL 60
           MFSHQRYCIMSVEEAYHTSKPIEDINDDLQVQSIIGQLAVEDHNKKTGDNLELVDVVNGL
Sbjct: 1   MFSHQRYCIMSVEEAYHTSKPIEDINDDLQVQSIIGQLAVEDHNKKTGDNLELVDVVNGL 60

Query: 61  RSGIFVQPGSTHEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRISCRPLFTVLCPATT 120
           RSGIFVQPGSTHEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRISCRPLFTVLCPATT
Sbjct: 61  RSGIFVQPGSTHEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRISCRPLFTVLCPATT 120

Query: 121 SIIEPTSIRQR 131
           SIIEPTSIRQR
Sbjct: 121 SIIEPTSIRQR 131

BLAST of CsGy4G018790 vs. NCBI nr
Match: TYK05428.1 (Proteinase inhibitor I25, cystatin [Cucumis melo var. makuwa])

HSP 1 Score: 187 bits (475), Expect = 7.83e-59
Identity = 91/98 (92.86%), Postives = 95/98 (96.94%), Query Frame = 0

Query: 10  MSVEEAYHTSKPIEDINDDLQVQSIIGQLAVEDHNKKTGDNLELVDVVNGLRSGIFVQPG 69
           MSVEEAYHTSKPIE+INDDLQVQSIIG+LAVEDHNKKTG+NLE VDVVNGLRS +FVQPG
Sbjct: 1   MSVEEAYHTSKPIENINDDLQVQSIIGKLAVEDHNKKTGENLEFVDVVNGLRSEVFVQPG 60

Query: 70  STHEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRIS 107
            THEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRIS
Sbjct: 61  CTHEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRIS 98

BLAST of CsGy4G018790 vs. NCBI nr
Match: KGN54823.1 (hypothetical protein Csa_012607 [Cucumis sativus])

HSP 1 Score: 89.0 bits (219), Expect = 6.38e-20
Identity = 52/95 (54.74%), Postives = 69/95 (72.63%), Query Frame = 0

Query: 10  MSVEEAYHTSKPIEDINDDLQVQSIIGQLAVED-HNKKTGDNLELVDVVNGLRSGIFVQP 69
           MS+EE+ H  KPI DIND   VQ++ G+LAVE+ ++K +G+ L+ V VVNGL+S  ++ P
Sbjct: 1   MSIEESRHRLKPIIDINDP-HVQAL-GELAVENLYSKNSGEKLKFVRVVNGLKSDKYIGP 60

Query: 70  GSTHEGILYHLLVEAKTIEGINWTYVAKLLELYVG 103
           G T E ILYHL++EAKT E INWTY  KL E+  G
Sbjct: 61  GFT-EVILYHLVLEAKTNEEINWTYATKLEEVSGG 92

BLAST of CsGy4G018790 vs. NCBI nr
Match: XP_008442277.1 (PREDICTED: cysteine proteinase inhibitor A-like [Cucumis melo] >KAA0041897.1 cysteine proteinase inhibitor A-like [Cucumis melo var. makuwa] >TYK05422.1 cysteine proteinase inhibitor A-like [Cucumis melo var. makuwa])

HSP 1 Score: 87.8 bits (216), Expect = 1.91e-19
Identity = 48/87 (55.17%), Postives = 61/87 (70.11%), Query Frame = 0

Query: 10 MSVEEAYHTSKPIEDINDDLQVQSIIGQLAVEDHNKKTGDNLELVDVVNGLRSGIFVQPG 69
          MS+  A+   KPIEDIND+L+VQ + G L V+DHNKKTG+NL+ V VVNGL +  F  P 
Sbjct: 1  MSIMSAF---KPIEDINDNLEVQDL-GNLTVDDHNKKTGENLKFVRVVNGLAAS-FSDPI 60

Query: 70 STHEGILYHLLVEAKTIEGINWTYVAK 96
          S   G+LY L++EA T   INWTY A+
Sbjct: 61 SPLGGVLYELVLEAITTSKINWTYKAR 82

BLAST of CsGy4G018790 vs. NCBI nr
Match: KAE8649716.1 (hypothetical protein Csa_012476 [Cucumis sativus])

HSP 1 Score: 78.2 bits (191), Expect = 1.07e-15
Identity = 49/95 (51.58%), Postives = 59/95 (62.11%), Query Frame = 0

Query: 20  KPIEDINDDLQVQSIIGQLAVEDHNKKTGDNLELVDVVNGLRSGIFVQPGSTHEGILYHL 79
           +PI+DIND  QVQ I G L V+DHNKKTG+NL+ V VVNGL +G FV P S   G +Y L
Sbjct: 8   EPIKDINDP-QVQEI-GNLTVDDHNKKTGENLKFVRVVNGLFAG-FVDPFSPLGGTVYEL 67

Query: 80  LVEAKTIEGINWTYVAKLLELYVGCRISCRPLFTV 114
           ++EA     INWTY  K     V    + RPL  V
Sbjct: 68  VLEAINAGKINWTYKVK-----VSASTTIRPLGVV 94

BLAST of CsGy4G018790 vs. ExPASy TrEMBL
Match: A0A0A0L3W1 (Proteinase inhibitor I25, cystatin OS=Cucumis sativus OX=3659 GN=Csa_4G508490 PE=4 SV=1)

HSP 1 Score: 218 bits (554), Expect = 5.05e-71
Identity = 106/106 (100.00%), Postives = 106/106 (100.00%), Query Frame = 0

Query: 1   MFSHQRYCIMSVEEAYHTSKPIEDINDDLQVQSIIGQLAVEDHNKKTGDNLELVDVVNGL 60
           MFSHQRYCIMSVEEAYHTSKPIEDINDDLQVQSIIGQLAVEDHNKKTGDNLELVDVVNGL
Sbjct: 1   MFSHQRYCIMSVEEAYHTSKPIEDINDDLQVQSIIGQLAVEDHNKKTGDNLELVDVVNGL 60

Query: 61  RSGIFVQPGSTHEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRI 106
           RSGIFVQPGSTHEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRI
Sbjct: 61  RSGIFVQPGSTHEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRI 106

BLAST of CsGy4G018790 vs. ExPASy TrEMBL
Match: A0A5D3C2L1 (Proteinase inhibitor I25, cystatin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold83G001230 PE=4 SV=1)

HSP 1 Score: 187 bits (475), Expect = 3.79e-59
Identity = 91/98 (92.86%), Postives = 95/98 (96.94%), Query Frame = 0

Query: 10  MSVEEAYHTSKPIEDINDDLQVQSIIGQLAVEDHNKKTGDNLELVDVVNGLRSGIFVQPG 69
           MSVEEAYHTSKPIE+INDDLQVQSIIG+LAVEDHNKKTG+NLE VDVVNGLRS +FVQPG
Sbjct: 1   MSVEEAYHTSKPIENINDDLQVQSIIGKLAVEDHNKKTGENLEFVDVVNGLRSEVFVQPG 60

Query: 70  STHEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRIS 107
            THEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRIS
Sbjct: 61  CTHEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRIS 98

BLAST of CsGy4G018790 vs. ExPASy TrEMBL
Match: A0A0A0L191 (Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G508520 PE=4 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 3.71e-24
Identity = 57/98 (58.16%), Postives = 70/98 (71.43%), Query Frame = 0

Query: 10  MSVEEAYHTSKPIEDINDDLQVQSIIGQLAVEDHNKKT-GDNLELVDVVNGLRSGIFVQP 69
           MS+EE +H  KPI DIND   VQ IIG+LAVEDH+    G+ L+ V VVNGL+S  ++ P
Sbjct: 1   MSIEEIHHRLKPIIDINDP-HVQ-IIGKLAVEDHDSHNPGEKLKFVRVVNGLKSDKYIGP 60

Query: 70  GSTHEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRI 106
           G T E ILYHL++EAKT E INWTY  KL ++Y GC I
Sbjct: 61  GFT-EVILYHLVLEAKTNEEINWTYATKLEKVYSGCLI 95

BLAST of CsGy4G018790 vs. ExPASy TrEMBL
Match: A0A0A0KZ98 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G508510 PE=4 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 3.09e-20
Identity = 52/95 (54.74%), Postives = 69/95 (72.63%), Query Frame = 0

Query: 10  MSVEEAYHTSKPIEDINDDLQVQSIIGQLAVED-HNKKTGDNLELVDVVNGLRSGIFVQP 69
           MS+EE+ H  KPI DIND   VQ++ G+LAVE+ ++K +G+ L+ V VVNGL+S  ++ P
Sbjct: 1   MSIEESRHRLKPIIDINDP-HVQAL-GELAVENLYSKNSGEKLKFVRVVNGLKSDKYIGP 60

Query: 70  GSTHEGILYHLLVEAKTIEGINWTYVAKLLELYVG 103
           G T E ILYHL++EAKT E INWTY  KL E+  G
Sbjct: 61  GFT-EVILYHLVLEAKTNEEINWTYATKLEEVSGG 92

BLAST of CsGy4G018790 vs. ExPASy TrEMBL
Match: A0A5A7TJK0 (Cysteine proteinase inhibitor A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold83G001140 PE=4 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 9.27e-20
Identity = 48/87 (55.17%), Postives = 61/87 (70.11%), Query Frame = 0

Query: 10 MSVEEAYHTSKPIEDINDDLQVQSIIGQLAVEDHNKKTGDNLELVDVVNGLRSGIFVQPG 69
          MS+  A+   KPIEDIND+L+VQ + G L V+DHNKKTG+NL+ V VVNGL +  F  P 
Sbjct: 1  MSIMSAF---KPIEDINDNLEVQDL-GNLTVDDHNKKTGENLKFVRVVNGLAAS-FSDPI 60

Query: 70 STHEGILYHLLVEAKTIEGINWTYVAK 96
          S   G+LY L++EA T   INWTY A+
Sbjct: 61 SPLGGVLYELVLEAITTSKINWTYKAR 82

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
XP_031739698.11.04e-90100.00uncharacterized protein LOC116403239 [Cucumis sativus] >KAE8649718.1 hypothetica... [more]
TYK05428.17.83e-5992.86Proteinase inhibitor I25, cystatin [Cucumis melo var. makuwa][more]
KGN54823.16.38e-2054.74hypothetical protein Csa_012607 [Cucumis sativus][more]
XP_008442277.11.91e-1955.17PREDICTED: cysteine proteinase inhibitor A-like [Cucumis melo] >KAA0041897.1 cys... [more]
KAE8649716.11.07e-1551.58hypothetical protein Csa_012476 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
A0A0A0L3W15.05e-71100.00Proteinase inhibitor I25, cystatin OS=Cucumis sativus OX=3659 GN=Csa_4G508490 PE... [more]
A0A5D3C2L13.79e-5992.86Proteinase inhibitor I25, cystatin OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A0A0L1913.71e-2458.16Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G508520 PE... [more]
A0A0A0KZ983.09e-2054.74Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G508510 PE=4 SV=1[more]
A0A5A7TJK09.27e-2055.17Cysteine proteinase inhibitor A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.10.450.10coord: 18..103
e-value: 2.7E-9
score: 38.9
NoneNo IPR availableSUPERFAMILY54403Cystatin/monellincoord: 22..99

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy4G018790.2CsGy4G018790.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010951 negative regulation of endopeptidase activity
molecular_function GO:0004869 cysteine-type endopeptidase inhibitor activity