CsGy4G017850 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy4G017850
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionRING-type domain-containing protein
LocationGy14Chr4: 23023288 .. 23024079 (+)
RNA-Seq ExpressionCsGy4G017850
SyntenyCsGy4G017850
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TGTTGATGCAGTCATCGTTTCAATTCAAATTTCTAGTTGAAGCTTTGTTTGACACCTTCAATGGAATATCAAATCTCTCGATACTCAACTACGGCAGTGGAAGTAGATATAGATGTTCCGAAGTGGACAAAAATCCCGAAGCATCCATCACCAATCGAACTAATTGGCTTGCTCTTCACCCTACGTCGTCGTTGCAACCGCTTTCAGGTGCTGGAGAACGGTGAACGACAATTAGTAGAGGAACAAGAGATATCAAAAGTTCCTCTATGCGACCACCAATTCCCTGTTGAAATATTTAATGGAGACAGCAGTTCTCTATTCCAATACCTCTGTTGTAGCTTCGAAAACTACGAATCTAAAATTGATGCAGAGGTGGTAGCAAGTGTCCTAATCGAGCAGTGGATTGAAATAAGGAACCAAGTGAATTCGAGAAGCCGAGATAAAGTATTTTCAATGACGGTGAATTTGACAATCACAGATGTTGTGCTTCAATATGTTGTTGGCGAAGACGGCAGCACATTTGGTGGAGAAGATCATAGAGTGGTTCCAGCATGCGACAAAGCTCTTAAGAAGATGTTGAAGAGATTGGAAATGAGAGAGGAAGATGAAAGAGTTTGTGTGATATGTTTGGATGAAGTTGGGAAAAAGAAGAATGGAAGGATTGGTTTGGAAATGCCTTGTTTACATGTGTTTCATGGGAAATGCATTGAAAATTGGTTCAAAAATAGCCATTGTTGTCCAATTTGTAGATTTGAAATGCCAATCAGTTCAGACTTAGATGAAGATTCTTGA

mRNA sequence

TGTTGATGCAGTCATCGTTTCAATTCAAATTTCTAGTTGAAGCTTTGTTTGACACCTTCAATGGAATATCAAATCTCTCGATACTCAACTACGGCAGTGGAAGTAGATATAGATGTTCCGAAGTGGACAAAAATCCCGAAGCATCCATCACCAATCGAACTAATTGGCTTGCTCTTCACCCTACGTCGTCGTTGCAACCGCTTTCAGGTGCTGGAGAACGGTGAACGACAATTAGTAGAGGAACAAGAGATATCAAAAGTTCCTCTATGCGACCACCAATTCCCTGTTGAAATATTTAATGGAGACAGCAGTTCTCTATTCCAATACCTCTGTTGTAGCTTCGAAAACTACGAATCTAAAATTGATGCAGAGGTGGTAGCAAGTGTCCTAATCGAGCAGTGGATTGAAATAAGGAACCAAGTGAATTCGAGAAGCCGAGATAAAGTATTTTCAATGACGGTGAATTTGACAATCACAGATGTTGTGCTTCAATATGTTGTTGGCGAAGACGGCAGCACATTTGGTGGAGAAGATCATAGAGTGGTTCCAGCATGCGACAAAGCTCTTAAGAAGATGTTGAAGAGATTGGAAATGAGAGAGGAAGATGAAAGAGTTTGTGTGATATGTTTGGATGAAGTTGGGAAAAAGAAGAATGGAAGGATTGGTTTGGAAATGCCTTGTTTACATGTGTTTCATGGGAAATGCATTGAAAATTGGTTCAAAAATAGCCATTGTTGTCCAATTTGTAGATTTGAAATGCCAATCAGTTCAGACTTAGATGAAGATTCTTGA

Coding sequence (CDS)

ATGGAATATCAAATCTCTCGATACTCAACTACGGCAGTGGAAGTAGATATAGATGTTCCGAAGTGGACAAAAATCCCGAAGCATCCATCACCAATCGAACTAATTGGCTTGCTCTTCACCCTACGTCGTCGTTGCAACCGCTTTCAGGTGCTGGAGAACGGTGAACGACAATTAGTAGAGGAACAAGAGATATCAAAAGTTCCTCTATGCGACCACCAATTCCCTGTTGAAATATTTAATGGAGACAGCAGTTCTCTATTCCAATACCTCTGTTGTAGCTTCGAAAACTACGAATCTAAAATTGATGCAGAGGTGGTAGCAAGTGTCCTAATCGAGCAGTGGATTGAAATAAGGAACCAAGTGAATTCGAGAAGCCGAGATAAAGTATTTTCAATGACGGTGAATTTGACAATCACAGATGTTGTGCTTCAATATGTTGTTGGCGAAGACGGCAGCACATTTGGTGGAGAAGATCATAGAGTGGTTCCAGCATGCGACAAAGCTCTTAAGAAGATGTTGAAGAGATTGGAAATGAGAGAGGAAGATGAAAGAGTTTGTGTGATATGTTTGGATGAAGTTGGGAAAAAGAAGAATGGAAGGATTGGTTTGGAAATGCCTTGTTTACATGTGTTTCATGGGAAATGCATTGAAAATTGGTTCAAAAATAGCCATTGTTGTCCAATTTGTAGATTTGAAATGCCAATCAGTTCAGACTTAGATGAAGATTCTTGA

Protein sequence

MEYQISRYSTTAVEVDIDVPKWTKIPKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVEEQEISKVPLCDHQFPVEIFNGDSSSLFQYLCCSFENYESKIDAEVVASVLIEQWIEIRNQVNSRSRDKVFSMTVNLTITDVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEMREEDERVCVICLDEVGKKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPISSDLDEDS*
Homology
BLAST of CsGy4G017850 vs. ExPASy Swiss-Prot
Match: Q9P0P0 (E3 ubiquitin-protein ligase RNF181 OS=Homo sapiens OX=9606 GN=RNF181 PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 4.4e-10
Identity = 30/86 (34.88%), Postives = 47/86 (54.65%), Query Frame = 0

Query: 158 DHRVVPACDKALKKMLKRLEMR-EEDERVCVICLDEVGKKKNGRIGLEMPCLHVFHGKCI 217
           DH + P   K + + L R  +R  + E  C +CL E  +++     +EMPC H+FH  CI
Sbjct: 47  DHHLPPPAAKTVVENLPRTVIRGSQAELKCPVCLLEFEEEET---AIEMPCHHLFHSSCI 106

Query: 218 ENWFKNSHCCPICRFEMPISSDLDED 243
             W   ++ CP+CR+E+P   D  E+
Sbjct: 107 LPWLSKTNSCPLCRYELPTDDDTYEE 129

BLAST of CsGy4G017850 vs. ExPASy Swiss-Prot
Match: Q9LQX2 (E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 2.2e-09
Identity = 27/56 (48.21%), Postives = 34/56 (60.71%), Query Frame = 0

Query: 183 ERVCVICLDEVGKKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPISSD 239
           E  CVICL+E   ++  +   EMPC H FHG CIE W      CP+CR+EMP+  D
Sbjct: 110 EGECVICLEEWKSEETVK---EMPCKHRFHGGCIEKWLGFHGSCPVCRYEMPVDGD 162

BLAST of CsGy4G017850 vs. ExPASy Swiss-Prot
Match: Q3T0W3 (E3 ubiquitin-protein ligase RNF181 OS=Bos taurus OX=9913 GN=RNF181 PE=2 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 2.2e-09
Identity = 30/86 (34.88%), Postives = 45/86 (52.33%), Query Frame = 0

Query: 158 DHRVVPACDKALKKMLKRLEMR-EEDERVCVICLDEVGKKKNGRIGLEMPCLHVFHGKCI 217
           DH + P   K   + L R  +R  + E  C +CL E  +++     +EMPC H+FH  CI
Sbjct: 47  DHHLPPPAAKTAVENLPRTVIRGSQAELKCPVCLLEFEEEET---AIEMPCHHLFHSNCI 106

Query: 218 ENWFKNSHCCPICRFEMPISSDLDED 243
             W   ++ CP+CR E+P   D  E+
Sbjct: 107 LPWLSKTNSCPLCRHELPTDDDTYEE 129

BLAST of CsGy4G017850 vs. ExPASy Swiss-Prot
Match: Q6AXU4 (E3 ubiquitin-protein ligase RNF181 OS=Rattus norvegicus OX=10116 GN=Rnf181 PE=1 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 3.7e-09
Identity = 30/86 (34.88%), Postives = 47/86 (54.65%), Query Frame = 0

Query: 158 DHRVVPACDKALKKMLKRLEMR-EEDERVCVICLDEVGKKKNGRIGLEMPCLHVFHGKCI 217
           +H + P   KA+ + L R  +R  + E  C +CL E  +++     +EMPC H+FH  CI
Sbjct: 59  EHHLPPPAAKAVVESLPRTVIRSSKAELKCPVCLLEFEEEET---VIEMPCHHLFHSNCI 118

Query: 218 ENWFKNSHCCPICRFEMPISSDLDED 243
             W   ++ CP+CR E+P   D  E+
Sbjct: 119 LPWLSKTNSCPLCRHELPTDDDSYEE 141

BLAST of CsGy4G017850 vs. ExPASy Swiss-Prot
Match: Q9SNB6 (E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 2.4e-08
Identity = 28/75 (37.33%), Postives = 41/75 (54.67%), Query Frame = 0

Query: 163 PACDKALKKMLKRLEMRE---EDERVCVICLDEVGKKKNGRIGLEMPCLHVFHGKCIENW 222
           P   K+  + L R+E+ +   + E  C +C +     + G  G EMPC H+FHG CI  W
Sbjct: 189 PPASKSAIESLPRVEISDCHTKAEANCAVCTEVF---EAGIEGREMPCKHIFHGDCIVPW 248

Query: 223 FKNSHCCPICRFEMP 235
               + CP+CRFE+P
Sbjct: 249 LSIRNSCPVCRFELP 260

BLAST of CsGy4G017850 vs. NCBI nr
Match: XP_011654386.1 (RING finger protein 141 [Cucumis sativus] >KGN54667.1 hypothetical protein Csa_012982 [Cucumis sativus])

HSP 1 Score: 505 bits (1300), Expect = 1.23e-180
Identity = 243/243 (100.00%), Postives = 243/243 (100.00%), Query Frame = 0

Query: 1   MEYQISRYSTTAVEVDIDVPKWTKIPKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60
           MEYQISRYSTTAVEVDIDVPKWTKIPKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE
Sbjct: 1   MEYQISRYSTTAVEVDIDVPKWTKIPKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60

Query: 61  EQEISKVPLCDHQFPVEIFNGDSSSLFQYLCCSFENYESKIDAEVVASVLIEQWIEIRNQ 120
           EQEISKVPLCDHQFPVEIFNGDSSSLFQYLCCSFENYESKIDAEVVASVLIEQWIEIRNQ
Sbjct: 61  EQEISKVPLCDHQFPVEIFNGDSSSLFQYLCCSFENYESKIDAEVVASVLIEQWIEIRNQ 120

Query: 121 VNSRSRDKVFSMTVNLTITDVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEMRE 180
           VNSRSRDKVFSMTVNLTITDVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEMRE
Sbjct: 121 VNSRSRDKVFSMTVNLTITDVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEMRE 180

Query: 181 EDERVCVICLDEVGKKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPISSDLD 240
           EDERVCVICLDEVGKKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPISSDLD
Sbjct: 181 EDERVCVICLDEVGKKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPISSDLD 240

Query: 241 EDS 243
           EDS
Sbjct: 241 EDS 243

BLAST of CsGy4G017850 vs. NCBI nr
Match: XP_016901804.1 (PREDICTED: RING finger protein 141-like [Cucumis melo] >KAA0025781.1 RING finger protein 141-like [Cucumis melo var. makuwa] >TYK09674.1 RING finger protein 141-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 438 bits (1126), Expect = 4.15e-154
Identity = 207/242 (85.54%), Postives = 223/242 (92.15%), Query Frame = 0

Query: 1   MEYQISRYSTTAVEVDIDVPKWTKIPKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60
           MEYQIS+YSTTAVE+DID+P   +I KHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE
Sbjct: 1   MEYQISQYSTTAVEIDIDLPMSRRIRKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60

Query: 61  EQEISKVPLCDHQFPVEIFNGDSSSLFQYLCCSFENYESKIDAEVVASVLIEQWIEIRNQ 120
           EQE SKVPLCDHQFPVEIFNGD +SLFQYLCCSFENYE KIDAEVVA+ LIEQWI IR+Q
Sbjct: 61  EQEASKVPLCDHQFPVEIFNGDDNSLFQYLCCSFENYEPKIDAEVVATALIEQWIGIRDQ 120

Query: 121 VNSRSRDKVFSMTVNLTITDVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEMRE 180
           +NSRS  KVFSMTVNLTITDVV+QYV+GEDGS+FGGED R VPACDKALKKMLKRLE+R+
Sbjct: 121 LNSRSCHKVFSMTVNLTITDVVVQYVIGEDGSSFGGEDFRAVPACDKALKKMLKRLEVRD 180

Query: 181 EDERVCVICLDEVGKKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPISSDLD 240
           EDER+C+ICLDEV KKKNGR GLEMPCLHVFHGKCIENW KNSHCCPICRF+MPISS   
Sbjct: 181 EDERICIICLDEVRKKKNGRCGLEMPCLHVFHGKCIENWLKNSHCCPICRFQMPISSGFG 240

Query: 241 ED 242
           ED
Sbjct: 241 ED 242

BLAST of CsGy4G017850 vs. NCBI nr
Match: KAG6571896.1 (E3 ubiquitin-protein ligase MPSR1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 295 bits (756), Expect = 6.70e-98
Identity = 146/237 (61.60%), Postives = 182/237 (76.79%), Query Frame = 0

Query: 1   MEYQISRYSTTAVEVDIDVPKWTKIPKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60
           ME+QISRY    VEV  D+P  ++IPKHPS IE IGL+FTLRRR + F+V ENGER+L++
Sbjct: 1   MEHQISRYENLVVEVLTDLPTPSRIPKHPSRIEHIGLIFTLRRRRSHFEVSENGERRLIQ 60

Query: 61  EQEISKVPLCDHQFPVEIFNGDSSSLFQYLCCSFENYESKIDAEVVASVLIEQWIEIRNQ 120
           ++E  K+PLCD+QF  EIFN D  SLF  L CSF+N++ +IDAE VA  LIE+WIEIR++
Sbjct: 61  QRETLKIPLCDNQFRAEIFNRDDDSLFHSLSCSFQNFKPEIDAEPVADALIERWIEIRDE 120

Query: 121 VNSRSRDKVFSMTVNLTITDVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEMRE 180
            NSRS D+VF MTVNLTI  +V +Y +G  GS  GG+   +VPAC  A+ KML+RLE  +
Sbjct: 121 ANSRSSDRVFPMTVNLTIWHIVARYEIGV-GS--GGDGCGMVPACGGAVGKMLRRLERED 180

Query: 181 EDERVCVICLDEVG--KKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPI 235
           E + +CVICLDE+   KKKNGR GL+MPCLHVFHG CIE+W KNSH CPICRFE+PI
Sbjct: 181 EVDGICVICLDELKNKKKKNGRFGLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVPI 234

BLAST of CsGy4G017850 vs. NCBI nr
Match: XP_022952293.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1-like [Cucurbita moschata] >KAG7011584.1 HRD1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 293 bits (750), Expect = 5.47e-97
Identity = 144/237 (60.76%), Postives = 182/237 (76.79%), Query Frame = 0

Query: 1   MEYQISRYSTTAVEVDIDVPKWTKIPKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60
           ME+QISRY    VEV  D+P  ++IPKHPS IE IGL+FTLRRR + F+V ENGER+L++
Sbjct: 1   MEHQISRYENLVVEVLTDLPTPSRIPKHPSRIEHIGLIFTLRRRRSHFEVSENGERRLIQ 60

Query: 61  EQEISKVPLCDHQFPVEIFNGDSSSLFQYLCCSFENYESKIDAEVVASVLIEQWIEIRNQ 120
           ++E  K+PLCD+QF  EIFN D  SLF  L CSF+N++ +IDAE VA  LIE+WIEIR++
Sbjct: 61  QRETLKIPLCDNQFRAEIFNRDDDSLFHSLSCSFQNFKPEIDAEPVADALIERWIEIRDE 120

Query: 121 VNSRSRDKVFSMTVNLTITDVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEMRE 180
            NSRS D+VF MTVNLT+  +V +Y +G  GS  GG+   +VPAC  A+ KML+RL+  +
Sbjct: 121 ANSRSSDRVFPMTVNLTMWHIVARYEIGV-GS--GGDGCGMVPACGGAVAKMLRRLKRED 180

Query: 181 EDERVCVICLDEVG--KKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPI 235
           E + +CVICLDE+   KKKNGR GL+MPCLHVFHG CIE+W KNSH CPICRFE+PI
Sbjct: 181 EVDGICVICLDELKNKKKKNGRFGLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVPI 234

BLAST of CsGy4G017850 vs. NCBI nr
Match: XP_023511497.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 291 bits (746), Expect = 2.22e-96
Identity = 144/237 (60.76%), Postives = 182/237 (76.79%), Query Frame = 0

Query: 1   MEYQISRYSTTAVEVDIDVPKWTKIPKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60
           ME+QISRY +  VEV  D+P  ++IPKHPS IE IGL+FTLRRR + F+V ENGER+L++
Sbjct: 1   MEHQISRYESLVVEVLTDLPTPSRIPKHPSRIEHIGLIFTLRRRRSHFEVSENGERRLIQ 60

Query: 61  EQEISKVPLCDHQFPVEIFNGDSSSLFQYLCCSFENYESKIDAEVVASVLIEQWIEIRNQ 120
           ++E  K+PLCD+QF  EIFN D  SLF  L CSF+N++ +IDAE VA  LIE+WIEIR++
Sbjct: 61  QRETLKIPLCDNQFRAEIFNRDDDSLFHSLSCSFQNFKPEIDAEPVADALIERWIEIRDE 120

Query: 121 VNSRSRDKVFSMTVNLTITDVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEMRE 180
            NSRS D+VF MTVNLTI  +V +Y +G  GS  GG+   +VPAC  A+ KML+RL+  +
Sbjct: 121 ANSRSSDRVFPMTVNLTIWHIVARYEIGV-GS--GGDGCGMVPACGGAVAKMLRRLKRED 180

Query: 181 EDERVCVICLDEVG--KKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPI 235
           E + +CVICLDE+   KKKNGR  L+MPCLHVFHG CIE+W KNSH CPICRFE+PI
Sbjct: 181 EVDGICVICLDELKNKKKKNGRFTLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVPI 234

BLAST of CsGy4G017850 vs. ExPASy TrEMBL
Match: A0A0A0L236 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G419590 PE=4 SV=1)

HSP 1 Score: 505 bits (1300), Expect = 5.97e-181
Identity = 243/243 (100.00%), Postives = 243/243 (100.00%), Query Frame = 0

Query: 1   MEYQISRYSTTAVEVDIDVPKWTKIPKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60
           MEYQISRYSTTAVEVDIDVPKWTKIPKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE
Sbjct: 1   MEYQISRYSTTAVEVDIDVPKWTKIPKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60

Query: 61  EQEISKVPLCDHQFPVEIFNGDSSSLFQYLCCSFENYESKIDAEVVASVLIEQWIEIRNQ 120
           EQEISKVPLCDHQFPVEIFNGDSSSLFQYLCCSFENYESKIDAEVVASVLIEQWIEIRNQ
Sbjct: 61  EQEISKVPLCDHQFPVEIFNGDSSSLFQYLCCSFENYESKIDAEVVASVLIEQWIEIRNQ 120

Query: 121 VNSRSRDKVFSMTVNLTITDVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEMRE 180
           VNSRSRDKVFSMTVNLTITDVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEMRE
Sbjct: 121 VNSRSRDKVFSMTVNLTITDVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEMRE 180

Query: 181 EDERVCVICLDEVGKKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPISSDLD 240
           EDERVCVICLDEVGKKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPISSDLD
Sbjct: 181 EDERVCVICLDEVGKKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPISSDLD 240

Query: 241 EDS 243
           EDS
Sbjct: 241 EDS 243

BLAST of CsGy4G017850 vs. ExPASy TrEMBL
Match: A0A5A7SNF1 (RING finger protein 141-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold447G00770 PE=4 SV=1)

HSP 1 Score: 438 bits (1126), Expect = 2.01e-154
Identity = 207/242 (85.54%), Postives = 223/242 (92.15%), Query Frame = 0

Query: 1   MEYQISRYSTTAVEVDIDVPKWTKIPKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60
           MEYQIS+YSTTAVE+DID+P   +I KHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE
Sbjct: 1   MEYQISQYSTTAVEIDIDLPMSRRIRKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60

Query: 61  EQEISKVPLCDHQFPVEIFNGDSSSLFQYLCCSFENYESKIDAEVVASVLIEQWIEIRNQ 120
           EQE SKVPLCDHQFPVEIFNGD +SLFQYLCCSFENYE KIDAEVVA+ LIEQWI IR+Q
Sbjct: 61  EQEASKVPLCDHQFPVEIFNGDDNSLFQYLCCSFENYEPKIDAEVVATALIEQWIGIRDQ 120

Query: 121 VNSRSRDKVFSMTVNLTITDVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEMRE 180
           +NSRS  KVFSMTVNLTITDVV+QYV+GEDGS+FGGED R VPACDKALKKMLKRLE+R+
Sbjct: 121 LNSRSCHKVFSMTVNLTITDVVVQYVIGEDGSSFGGEDFRAVPACDKALKKMLKRLEVRD 180

Query: 181 EDERVCVICLDEVGKKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPISSDLD 240
           EDER+C+ICLDEV KKKNGR GLEMPCLHVFHGKCIENW KNSHCCPICRF+MPISS   
Sbjct: 181 EDERICIICLDEVRKKKNGRCGLEMPCLHVFHGKCIENWLKNSHCCPICRFQMPISSGFG 240

Query: 241 ED 242
           ED
Sbjct: 241 ED 242

BLAST of CsGy4G017850 vs. ExPASy TrEMBL
Match: A0A1S4E0Q9 (RING finger protein 141-like OS=Cucumis melo OX=3656 GN=LOC103495859 PE=4 SV=1)

HSP 1 Score: 438 bits (1126), Expect = 2.01e-154
Identity = 207/242 (85.54%), Postives = 223/242 (92.15%), Query Frame = 0

Query: 1   MEYQISRYSTTAVEVDIDVPKWTKIPKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60
           MEYQIS+YSTTAVE+DID+P   +I KHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE
Sbjct: 1   MEYQISQYSTTAVEIDIDLPMSRRIRKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60

Query: 61  EQEISKVPLCDHQFPVEIFNGDSSSLFQYLCCSFENYESKIDAEVVASVLIEQWIEIRNQ 120
           EQE SKVPLCDHQFPVEIFNGD +SLFQYLCCSFENYE KIDAEVVA+ LIEQWI IR+Q
Sbjct: 61  EQEASKVPLCDHQFPVEIFNGDDNSLFQYLCCSFENYEPKIDAEVVATALIEQWIGIRDQ 120

Query: 121 VNSRSRDKVFSMTVNLTITDVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEMRE 180
           +NSRS  KVFSMTVNLTITDVV+QYV+GEDGS+FGGED R VPACDKALKKMLKRLE+R+
Sbjct: 121 LNSRSCHKVFSMTVNLTITDVVVQYVIGEDGSSFGGEDFRAVPACDKALKKMLKRLEVRD 180

Query: 181 EDERVCVICLDEVGKKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPISSDLD 240
           EDER+C+ICLDEV KKKNGR GLEMPCLHVFHGKCIENW KNSHCCPICRF+MPISS   
Sbjct: 181 EDERICIICLDEVRKKKNGRCGLEMPCLHVFHGKCIENWLKNSHCCPICRFQMPISSGFG 240

Query: 241 ED 242
           ED
Sbjct: 241 ED 242

BLAST of CsGy4G017850 vs. ExPASy TrEMBL
Match: A0A6J1GK53 (ERAD-associated E3 ubiquitin-protein ligase HRD1-like OS=Cucurbita moschata OX=3662 GN=LOC111455016 PE=4 SV=1)

HSP 1 Score: 293 bits (750), Expect = 2.65e-97
Identity = 144/237 (60.76%), Postives = 182/237 (76.79%), Query Frame = 0

Query: 1   MEYQISRYSTTAVEVDIDVPKWTKIPKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60
           ME+QISRY    VEV  D+P  ++IPKHPS IE IGL+FTLRRR + F+V ENGER+L++
Sbjct: 1   MEHQISRYENLVVEVLTDLPTPSRIPKHPSRIEHIGLIFTLRRRRSHFEVSENGERRLIQ 60

Query: 61  EQEISKVPLCDHQFPVEIFNGDSSSLFQYLCCSFENYESKIDAEVVASVLIEQWIEIRNQ 120
           ++E  K+PLCD+QF  EIFN D  SLF  L CSF+N++ +IDAE VA  LIE+WIEIR++
Sbjct: 61  QRETLKIPLCDNQFRAEIFNRDDDSLFHSLSCSFQNFKPEIDAEPVADALIERWIEIRDE 120

Query: 121 VNSRSRDKVFSMTVNLTITDVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEMRE 180
            NSRS D+VF MTVNLT+  +V +Y +G  GS  GG+   +VPAC  A+ KML+RL+  +
Sbjct: 121 ANSRSSDRVFPMTVNLTMWHIVARYEIGV-GS--GGDGCGMVPACGGAVAKMLRRLKRED 180

Query: 181 EDERVCVICLDEVG--KKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPI 235
           E + +CVICLDE+   KKKNGR GL+MPCLHVFHG CIE+W KNSH CPICRFE+PI
Sbjct: 181 EVDGICVICLDELKNKKKKNGRFGLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVPI 234

BLAST of CsGy4G017850 vs. ExPASy TrEMBL
Match: A0A6J1I8N9 (ERAD-associated E3 ubiquitin-protein ligase HRD1-like OS=Cucurbita maxima OX=3661 GN=LOC111471014 PE=4 SV=1)

HSP 1 Score: 287 bits (734), Expect = 8.76e-95
Identity = 142/240 (59.17%), Postives = 181/240 (75.42%), Query Frame = 0

Query: 1   MEYQISRYSTTAVEVDIDVPKWTKIPKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVE 60
           ME+QISRY    VEV  D+P  ++IPKHPS IE IGL+FTLR R + F+V E+GER+L++
Sbjct: 1   MEHQISRYENLVVEVLTDLPTPSRIPKHPSRIEHIGLIFTLRHRRSHFEVSESGERRLIQ 60

Query: 61  EQEISKVPLCDHQFPVEIFNGDSSSLFQYLCCSFENYESKIDAEVVASVLIEQWIEIRNQ 120
           ++E  K+PLCD+QF  EIFN D  SLF  L  SF+N++ +IDAE VA  LIE+WIEIR++
Sbjct: 61  QRETLKIPLCDNQFRAEIFNRDDDSLFHNLSSSFQNFKPEIDAEPVADALIERWIEIRDE 120

Query: 121 VNSRSRDKVFSMTVNLTITDVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEMRE 180
            NSRS D+VF MTVNLT+  +V +Y +G  GS  GG+   +VPAC  A+ KML+RLE  +
Sbjct: 121 ANSRSSDRVFPMTVNLTMWHIVARYEIGV-GS--GGDGCGMVPACGGAVGKMLRRLERED 180

Query: 181 EDERVCVICLDEVGKKK--NGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPISSD 238
           E + +CVICLDE+ KKK  NGR GL+MPCLHVFHG CIE+W KNSH CPICRFE+PI  +
Sbjct: 181 EVDGICVICLDELKKKKKKNGRFGLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVPIQKN 237

BLAST of CsGy4G017850 vs. TAIR 10
Match: AT1G21960.1 (RING/U-box superfamily protein )

HSP 1 Score: 72.0 bits (175), Expect = 7.4e-13
Identity = 45/158 (28.48%), Postives = 74/158 (46.84%), Query Frame = 0

Query: 87  FQYLCCSFENYESKIDAEVVASVLIEQWIEIRNQVNSRSRDKVFSMTVNLTITDVVLQY- 146
           ++ +  S+ N   K++  + ++ L EQ+++I N   +    K  SM  +     +V  Y 
Sbjct: 49  YEGVISSYPNDSKKLNDWLYSAGLSEQYVDIANSQLTERICKSISMVDSSVCATLVYMYC 108

Query: 147 --VVGEDGSTFGGEDHRVV---PA----CDKALKKMLKRLEMREEDERVCVICLDEVGKK 206
             +V     +F    + +    PA     D+  +K+  + +    D  VC ICL+E  K 
Sbjct: 109 KVIVPPRIDSFDETTNNIELIRPANKLVVDRLARKVYNKKKKSNSDASVCTICLEEFEK- 168

Query: 207 KNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMP 235
             G I + +PC H F   CI  WF   H CP+CRFE+P
Sbjct: 169 --GEIVVTLPCGHEFDDGCIGKWFLKDHVCPLCRFELP 203

BLAST of CsGy4G017850 vs. TAIR 10
Match: AT1G14200.1 (RING/U-box superfamily protein )

HSP 1 Score: 70.5 bits (171), Expect = 2.2e-12
Identity = 37/96 (38.54%), Postives = 52/96 (54.17%), Query Frame = 0

Query: 149 EDGSTFGGEDHRVVPACDKALKKMLKRLEMREEDERV---CVICLDEVGKKKNGRIGLEM 208
           +D  + GG+  R  PA    ++ M  R+ + E+ E+    C ICLDE  K   G +  EM
Sbjct: 71  DDFFSDGGKQGR-SPALKSEVENM-PRVVIGEDKEKYGGSCAICLDEWSK---GDVAAEM 130

Query: 209 PCLHVFHGKCIENWFKNSHCCPICRFEMPISSDLDE 242
           PC H FH KC+E W      CP+CR+EMP+    +E
Sbjct: 131 PCKHKFHSKCVEEWLGRHATCPMCRYEMPVEEVEEE 161

BLAST of CsGy4G017850 vs. TAIR 10
Match: AT3G15740.1 (RING/U-box superfamily protein )

HSP 1 Score: 70.5 bits (171), Expect = 2.2e-12
Identity = 39/100 (39.00%), Postives = 52/100 (52.00%), Query Frame = 0

Query: 143 LQYVVGEDGSTFGGEDHR--------VVPACDKALKKMLKRLEMREEDERVCVICLDEVG 202
           +Q  +G   S F GED          V PA D+A++  L+ L +  E+E  CVIC+D + 
Sbjct: 127 VQVKIGVTVSRFPGEDDHVDVQVQLAVAPANDEAVEMHLETLVV--ENEGYCVICMDNIR 186

Query: 203 KKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMP 235
              +   G  MPC HVFH  C E W +NS  CP+CR   P
Sbjct: 187 VGSDVEAG-RMPCSHVFHRTCGEEWLRNSGICPVCRALFP 223

BLAST of CsGy4G017850 vs. TAIR 10
Match: AT5G56340.1 (RING/U-box superfamily protein )

HSP 1 Score: 65.9 bits (159), Expect = 5.3e-11
Identity = 35/104 (33.65%), Postives = 55/104 (52.88%), Query Frame = 0

Query: 140 DVVLQYVVGEDGSTFGGEDHRVVPACDKALKKMLKRLEMREEDERVCVICLDEVGKKKNG 199
           D++LQ++   D +  G       P   K   + L  +++ E  +  C +CLD+  K   G
Sbjct: 221 DLLLQHLAENDPNRQG------TPPARKEAVEALPTVKIMEPLQ--CSVCLDDFEK---G 280

Query: 200 RIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPISSDLDEDS 244
               EMPC H FH +CI  W +    CP+CRFE+P S+D D+++
Sbjct: 281 TEAKEMPCKHKFHVRCIVPWLELHSSCPVCRFELPSSADDDDET 313

BLAST of CsGy4G017850 vs. TAIR 10
Match: AT1G26800.1 (RING/U-box superfamily protein )

HSP 1 Score: 64.3 bits (155), Expect = 1.5e-10
Identity = 27/56 (48.21%), Postives = 34/56 (60.71%), Query Frame = 0

Query: 183 ERVCVICLDEVGKKKNGRIGLEMPCLHVFHGKCIENWFKNSHCCPICRFEMPISSD 239
           E  CVICL+E   ++  +   EMPC H FHG CIE W      CP+CR+EMP+  D
Sbjct: 110 EGECVICLEEWKSEETVK---EMPCKHRFHGGCIEKWLGFHGSCPVCRYEMPVDGD 162

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9P0P04.4e-1034.88E3 ubiquitin-protein ligase RNF181 OS=Homo sapiens OX=9606 GN=RNF181 PE=1 SV=1[more]
Q9LQX22.2e-0948.21E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 ... [more]
Q3T0W32.2e-0934.88E3 ubiquitin-protein ligase RNF181 OS=Bos taurus OX=9913 GN=RNF181 PE=2 SV=1[more]
Q6AXU43.7e-0934.88E3 ubiquitin-protein ligase RNF181 OS=Rattus norvegicus OX=10116 GN=Rnf181 PE=1 ... [more]
Q9SNB62.4e-0837.33E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 ... [more]
Match NameE-valueIdentityDescription
XP_011654386.11.23e-180100.00RING finger protein 141 [Cucumis sativus] >KGN54667.1 hypothetical protein Csa_0... [more]
XP_016901804.14.15e-15485.54PREDICTED: RING finger protein 141-like [Cucumis melo] >KAA0025781.1 RING finger... [more]
KAG6571896.16.70e-9861.60E3 ubiquitin-protein ligase MPSR1, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022952293.15.47e-9760.76ERAD-associated E3 ubiquitin-protein ligase HRD1-like [Cucurbita moschata] >KAG7... [more]
XP_023511497.12.22e-9660.76ERAD-associated E3 ubiquitin-protein ligase HRD1-like [Cucurbita pepo subsp. pep... [more]
Match NameE-valueIdentityDescription
A0A0A0L2365.97e-181100.00RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G419590 P... [more]
A0A5A7SNF12.01e-15485.54RING finger protein 141-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S4E0Q92.01e-15485.54RING finger protein 141-like OS=Cucumis melo OX=3656 GN=LOC103495859 PE=4 SV=1[more]
A0A6J1GK532.65e-9760.76ERAD-associated E3 ubiquitin-protein ligase HRD1-like OS=Cucurbita moschata OX=3... [more]
A0A6J1I8N98.76e-9559.17ERAD-associated E3 ubiquitin-protein ligase HRD1-like OS=Cucurbita maxima OX=366... [more]
Match NameE-valueIdentityDescription
AT1G21960.17.4e-1328.48RING/U-box superfamily protein [more]
AT1G14200.12.2e-1238.54RING/U-box superfamily protein [more]
AT3G15740.12.2e-1239.00RING/U-box superfamily protein [more]
AT5G56340.15.3e-1133.65RING/U-box superfamily protein [more]
AT1G26800.11.5e-1048.21RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011016Zinc finger, RING-CH-typeSMARTSM00744ringv_2coord: 185..230
e-value: 0.0042
score: 10.4
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 186..229
e-value: 1.1E-5
score: 34.8
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 186..230
e-value: 6.4E-11
score: 42.4
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 186..230
score: 12.046688
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 162..237
e-value: 5.2E-16
score: 59.6
NoneNo IPR availablePANTHERPTHR15710E3 UBIQUITIN-PROTEIN LIGASE PRAJAcoord: 104..234
NoneNo IPR availablePANTHERPTHR15710:SF63RING/U-BOX SUPERFAMILY PROTEINcoord: 104..234
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 175..236

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy4G017850.1CsGy4G017850.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005737 cytoplasm
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0008270 zinc ion binding