CsGy4G017820 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy4G017820
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionE3 ubiquitin-protein ligase
LocationGy14Chr4: 23002601 .. 23006260 (-)
RNA-Seq ExpressionCsGy4G017820
SyntenyCsGy4G017820
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTCCCACTCTCACTGCAACTCCTCGCTTTCTTCACCGGCGCCTTTTCCCTTCTCTCCGATCACCTCTCCCCGCTCCCATCACTCTTCGCCCAACTTCTCTTTTCATGGACGACGTTCGACCATCCAACTCTGGTTTCTTGTCCTGGCAGCGAACCATGAGCTCCAGATTCGCGCTTCCCTTGATTCAAGACGCTGCAAATTTGATCATGGAACATCGCGGCGTAATATTTGGTGACTGTGCCGATCCTCGAGTCGACGATGACGAAGATGATAATGAATACGAGCGTCGTGTTCTATTCCGGAATGGGTCTTCCTTTATCAAGCCTATTTTGGTACTCGACTTGTTCTGGAATTTGTTCTTCGTCTTTTTATCTGTCATAGTGCTGGTATTGTCTGCAGAAGAGAAACCTACTGCTCCTTTGAGATTCTGGCTTTCGGGTTATGCTGTTCAGTGCCTTTTCCATGTCTTTTTTGTGTTCGTTGCTTACTTGAGAAGGAGTAGTCGCTATCGACTTGGTTTTGAGAATCGTGGGGCTCAGGATGAACTTCGCCTTTCTCACAATCGTATAAGGTAACTTCTGTTTTGAGGAAAATTTTGTCGGGGTGGGGTTTTTGGTTTGAATTGGTTTCTCTTTTATGTCTAATTTTAGACTGTATTCGGTTTTCCGATGAGTTTACTTATTGGGTTCTTAGAATGATTGTTATGTGAAGATTTTCTGCTACTTTGTGGGGATTTTCATCGAATAGATTACGATCAGTTTAATTAACTATCGGATTATATGCATGTGTCATCCCATATATTATTTTTTTTTTCATTTTCTTCATGGTGTTGGGAGGGGGGAAAGAAAACTACCCAAGTCGTTGGCATAGAGTTCTTTTGGATGTTGAGCTAACTTGATGAATTGAGTTCTACTAGTTTAATTTCGAAGGGATTCTATATTTTGACAGTCGGTTGAATTGAGTATTCTTTTCCATGTCAAATCATAAATTGATACTTTTAGAATGTAGGAGGAAAGTTTTGGTGTCTAATGCAACATTGTTACTGAATTCAGCCATTACTAATGAGGGTGAACTTAGTGACAAGTTTGATAGTTTTGTTATGGTTCTTGTAGAAAAAAAGACATGTTTATTTGAACTTTTTGCTTTTAAAGTGGTTACTATGTGCAGTATACTTAATTTATATTTGGAATGATGTTTTTTCGTCTCATGTAAAGAATGTAGCACGTATTATATATGGTAATGAATTTGATTATGTATGCTAAGAAACTATGTATCACCTAGGTACTTTGGGGTTTCCCCTTGGTAAAGGACTTGCTTAGGTATCTTTATTTCATTTATGGTAATAAATCTTGTTTCTCCATTTAAAAATTTATTGGATTAGGTTTTTGGTTTCCATGTCTCTGAAGGATTTTACACTTATTAGCAGGAATTTATTTCCAGTCAAGATATTTGCACGTTTACTTATTTTTCTTCTTGTGACAATACATGTAGATGTTTCATCAATCTTTGTATTTTCAGTCTTGTTCAACTCTCTTTTTACCCCCTCTGTTTTTTCCCTAGAGTTATGAAAAGGTTGGAAGCACTGAACACAATGGTAGCATATATCTGGTGGGTATTTGGATTCTATTGGATTGTCATGGGTGGCCAAGCTCTTCTGGAAGGTTCTCCCCGACTGTACTGGTTAATACTCTCTCTTAGGGTCTTGAAACCCTTTTCTTTCTCTACCTAACCATGCCTGTGGTTTATCAGCAAATTAGTAGAATAATGATGTAATATGGAATATACCAGTTTGCTTGAAATGTATTCGCTCTCCATCACTAGCTATCGCAGCTTCTAAAATTCCATTAGATTTACTAGTTTCTCAAATTACTTTGTGAGAATAAATAATGCATCTTCATTTTGTGATCTCGATTCCGATATAACTTCCCGTAAATGTGTGAGTCTAAAATATGTTATTTTGTGATAAATGCGCTTTCCTTTTCACTAACTCCTGTTCATCACTGAAAGAAGCACTCAGGTCGTGTTTTCATATTATTTTCTAGTTACTTTGAGTTTCTGCTTCTGGACCTGTGGTTATATTTCCCTTGAGTTCTACATTGTTTCTTGGTGATACCTTTTTTGTTGTTGTTGTTGTTGTCGTTGTCGTTATCATTTCAATATCATTGTCATATTTGTATGAAAACTTCTCGTTTGTCTTGATGGAAGTCAAATATGAGGCTTCACTGATGGACTCATGATATCAAATGCAGGTTGGCAGTTGTTTTCCTAGCCTTTGATGTGTTTTTTATTATCTTCTGCACCGGGATGGCGTTTGTTGGTTTCTTTGCTGTTTGCTGCATAATACCATTTTTAGCATATGGTTATACCATGAATTTCCTAGAAGGTGCCTCTGAGGATGAAATTAGGGCACTTCCCAAGTACAGGTTTCACCAAGACAATCCACTTGAGTCGTTCGACAATGACAAGAAACAAGAAGTTGGGATGACATTGGAGCCTGGCTACAATGGTCATACAACTGAACACACTCTAAATGCAGAGGACTCGGTGAGCAACAATCTTATTTGTACTTTCATTCTCTTTTCATACCACCATGGATAAATGAAAATTTTTCTATCTCAGTTTGTTTCTAAATTCTAAAGAATTTCTACCACAAGAGTATTTATGAAACATTTATGAAAAGTTCAAGGGTAATATTACAACTTTTGAAAGTATAAGAATTTTTTAAAAATAAATGCCAAAGTTCAAGGATATTTGTTATAATTTAACCAATAATTTATCTGCACAGTTTCTACTTTTACCCCGTATCATCATACTCATAAAATAGACATCTTGTATTGTTACTTCGACAAAATTTTCCTATTTTTACATGTATACCATCAACAAAGGTATCCTGGTTACCTTTACAAGATATTTCTCCATGGAGTTTTCTTCTTTTAAAATGCTTTATCTGACACAAACTGAATCATGATTCCTTGTTTTAGAAGAATCTTTTTTTATCCTTCATAGTGCCTTTTATCATAGCATTGTAGGACTCTTTGCTATTTGATTAACTCTATGGTATTATATTTGCAGGCATGCTGCATATGCCTTGCTCAGTATGTACATGGAGTTCAATTGTGCATGCTTCCTTGTAATCATCATTTCCATACTCGATGCATTGTCAAATGGCTTCGAATAAACGCAACCTGCCCTCTCTGCAAGTTCAGCATCGGACAAGGTGATTCCTTGGTCTGATTCAACTTTTGATCAAGTCGGAGCATTCTTGAGGCTGGGACAAGATCTTTGACAACATAACCACACACAGGTCACGTATATCATCTCTTAACATATGTTGATTTGAGATTGAGACGGGTAGGGCAATGGGAGGGTAAAGCCTTGTTGAAAGGGAGGCCTTGCCTTTTCTTCTTTTTTTCTCTCTCTTTGAAAAGAGGAAATAATTTCCTTAATGGCCAAATTGTAGATAGCTTCTTTCTTTCGTTTTGAGATTACTTAGGAGGCCAGATTAGTGGGGAATACTGTGAATAGAATTTGATGCACAGCTTACTAACTTATATATGCTACTAAGAAGACTACTTAGTTGATAGTAACAATTTCAGTAAATAAAGGCAAAGAAGCCATTTGCTTGCTCAATATTAAGCCCATATCATATTGTTCAGTGT

mRNA sequence

ATTCCCACTCTCACTGCAACTCCTCGCTTTCTTCACCGGCGCCTTTTCCCTTCTCTCCGATCACCTCTCCCCGCTCCCATCACTCTTCGCCCAACTTCTCTTTTCATGGACGACGTTCGACCATCCAACTCTGGTTTCTTGTCCTGGCAGCGAACCATGAGCTCCAGATTCGCGCTTCCCTTGATTCAAGACGCTGCAAATTTGATCATGGAACATCGCGGCGTAATATTTGGTGACTGTGCCGATCCTCGAGTCGACGATGACGAAGATGATAATGAATACGAGCGTCGTGTTCTATTCCGGAATGGGTCTTCCTTTATCAAGCCTATTTTGGTACTCGACTTGTTCTGGAATTTGTTCTTCGTCTTTTTATCTGTCATAGTGCTGGTATTGTCTGCAGAAGAGAAACCTACTGCTCCTTTGAGATTCTGGCTTTCGGGTTATGCTGTTCAGTGCCTTTTCCATGTCTTTTTTGTGTTCGTTGCTTACTTGAGAAGGAGTAGTCGCTATCGACTTGGTTTTGAGAATCGTGGGGCTCAGGATGAACTTCGCCTTTCTCACAATCGTATAAGAGTTATGAAAAGGTTGGAAGCACTGAACACAATGGTAGCATATATCTGGTGGGTATTTGGATTCTATTGGATTGTCATGGGTGGCCAAGCTCTTCTGGAAGGTTCTCCCCGACTGTACTGGTTGGCAGTTGTTTTCCTAGCCTTTGATGTGTTTTTTATTATCTTCTGCACCGGGATGGCGTTTGTTGGTTTCTTTGCTGTTTGCTGCATAATACCATTTTTAGCATATGGTTATACCATGAATTTCCTAGAAGGTGCCTCTGAGGATGAAATTAGGGCACTTCCCAAGTACAGGTTTCACCAAGACAATCCACTTGAGTCGTTCGACAATGACAAGAAACAAGAAGTTGGGATGACATTGGAGCCTGGCTACAATGGTCATACAACTGAACACACTCTAAATGCAGAGGACTCGGCATGCTGCATATGCCTTGCTCAGTATGTACATGGAGTTCAATTGTGCATGCTTCCTTGTAATCATCATTTCCATACTCGATGCATTGTCAAATGGCTTCGAATAAACGCAACCTGCCCTCTCTGCAAGTTCAGCATCGGACAAGGTGATTCCTTGGTCTGATTCAACTTTTGATCAAGTCGGAGCATTCTTGAGGCTGGGACAAGATCTTTGACAACATAACCACACACAGGTCACGTATATCATCTCTTAACATATGTTGATTTGAGATTGAGACGGGTAGGGCAATGGGAGGGTAAAGCCTTGTTGAAAGGGAGGCCTTGCCTTTTCTTCTTTTTTTCTCTCTCTTTGAAAAGAGGAAATAATTTCCTTAATGGCCAAATTGTAGATAGCTTCTTTCTTTCGTTTTGAGATTACTTAGGAGGCCAGATTAGTGGGGAATACTGTGAATAGAATTTGATGCACAGCTTACTAACTTATATATGCTACTAAGAAGACTACTTAGTTGATAGTAACAATTTCAGTAAATAAAGGCAAAGAAGCCATTTGCTTGCTCAATATTAAGCCCATATCATATTGTTCAGTGT

Coding sequence (CDS)

ATGGACGACGTTCGACCATCCAACTCTGGTTTCTTGTCCTGGCAGCGAACCATGAGCTCCAGATTCGCGCTTCCCTTGATTCAAGACGCTGCAAATTTGATCATGGAACATCGCGGCGTAATATTTGGTGACTGTGCCGATCCTCGAGTCGACGATGACGAAGATGATAATGAATACGAGCGTCGTGTTCTATTCCGGAATGGGTCTTCCTTTATCAAGCCTATTTTGGTACTCGACTTGTTCTGGAATTTGTTCTTCGTCTTTTTATCTGTCATAGTGCTGGTATTGTCTGCAGAAGAGAAACCTACTGCTCCTTTGAGATTCTGGCTTTCGGGTTATGCTGTTCAGTGCCTTTTCCATGTCTTTTTTGTGTTCGTTGCTTACTTGAGAAGGAGTAGTCGCTATCGACTTGGTTTTGAGAATCGTGGGGCTCAGGATGAACTTCGCCTTTCTCACAATCGTATAAGAGTTATGAAAAGGTTGGAAGCACTGAACACAATGGTAGCATATATCTGGTGGGTATTTGGATTCTATTGGATTGTCATGGGTGGCCAAGCTCTTCTGGAAGGTTCTCCCCGACTGTACTGGTTGGCAGTTGTTTTCCTAGCCTTTGATGTGTTTTTTATTATCTTCTGCACCGGGATGGCGTTTGTTGGTTTCTTTGCTGTTTGCTGCATAATACCATTTTTAGCATATGGTTATACCATGAATTTCCTAGAAGGTGCCTCTGAGGATGAAATTAGGGCACTTCCCAAGTACAGGTTTCACCAAGACAATCCACTTGAGTCGTTCGACAATGACAAGAAACAAGAAGTTGGGATGACATTGGAGCCTGGCTACAATGGTCATACAACTGAACACACTCTAAATGCAGAGGACTCGGCATGCTGCATATGCCTTGCTCAGTATGTACATGGAGTTCAATTGTGCATGCTTCCTTGTAATCATCATTTCCATACTCGATGCATTGTCAAATGGCTTCGAATAAACGCAACCTGCCCTCTCTGCAAGTTCAGCATCGGACAAGGTGATTCCTTGGTCTGA

Protein sequence

MDDVRPSNSGFLSWQRTMSSRFALPLIQDAANLIMEHRGVIFGDCADPRVDDDEDDNEYERRVLFRNGSSFIKPILVLDLFWNLFFVFLSVIVLVLSAEEKPTAPLRFWLSGYAVQCLFHVFFVFVAYLRRSSRYRLGFENRGAQDELRLSHNRIRVMKRLEALNTMVAYIWWVFGFYWIVMGGQALLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFAVCCIIPFLAYGYTMNFLEGASEDEIRALPKYRFHQDNPLESFDNDKKQEVGMTLEPGYNGHTTEHTLNAEDSACCICLAQYVHGVQLCMLPCNHHFHTRCIVKWLRINATCPLCKFSIGQGDSLV*
Homology
BLAST of CsGy4G017820 vs. ExPASy Swiss-Prot
Match: Q9LN71 (E3 ubiquitin-protein ligase At1g12760 OS=Arabidopsis thaliana OX=3702 GN=At1g12760 PE=1 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 1.8e-62
Identity = 130/310 (41.94%), Postives = 177/310 (57.10%), Query Frame = 0

Query: 70  SFIKPILVLDLFWNLFFVFLSVIVLVLSAEEKPTAPLRFWLSGYAVQCLFHVFFVFVAYL 129
           ++ KP++VLD+ WNL FV ++  +LV+S +E P  PLR WL GYA+QC+ H+  V V Y 
Sbjct: 95  AYSKPVVVLDIVWNLAFVSVATAILVMSRKEHPIMPLRVWLLGYALQCVLHMVCVCVEYR 154

Query: 130 RRSSR---------------------------YRLGFENRGAQDELRLSH---NRIRVMK 189
           RR+ R                             LG        +L L H       V K
Sbjct: 155 RRNRRRTNRTTTTTPPRSRSSSSSSSSSSLEEEALGSRRNSGVQDLSLGHLDTESSSVAK 214

Query: 190 RLEALNTMVAYIWWVFGFYWIVMGGQALLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVG 249
            LE+ NTM ++IWW+ GFYW+  GGQ L + SPR+YWL++VFL FDVFF++FC  +A V 
Sbjct: 215 HLESANTMFSFIWWIIGFYWVSAGGQELAQESPRIYWLSIVFLGFDVFFVVFCVALACVI 274

Query: 250 FFAVCCIIP-FLAYGYTMNFLEGASEDEIRALPKYRFHQDNPLESFDNDKKQEV--GMTL 309
             AVCC +P  +A  Y +   EGAS+++I  L K++F +        ND+ Q    G+  
Sbjct: 275 GIAVCCCLPCIIAVLYAVADQEGASKEDIEQLTKFKFRKLGDANKHTNDEAQGTTEGIMT 334

Query: 310 EPGYNGHTTEHTLNAEDSACCICLAQYVHGVQLCMLPCNHHFHTRCIVKWLRINATCPLC 347
           E G +    EHTL  ED+ CCICL+ Y  G +L  LPC HHFH  C+ KWL INATCPLC
Sbjct: 335 ECGTDS-PIEHTLLQEDAECCICLSAYEDGTELRELPCGHHFHCSCVDKWLYINATCPLC 394

BLAST of CsGy4G017820 vs. ExPASy Swiss-Prot
Match: Q93Z92 (E3 ubiquitin-protein ligase At4g11680 OS=Arabidopsis thaliana OX=3702 GN=At4g11680 PE=2 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 1.8e-62
Identity = 126/283 (44.52%), Postives = 171/283 (60.42%), Query Frame = 0

Query: 70  SFIKPILVLDLFWNLFFVFLSVIVLVLSAEEKPTAPLRFWLSGYAVQCLFHVFFVFVAYL 129
           ++ KP++ LD+ WNL FV + V VL+LS +EKP  PLR W+ GY +QC  H+  V V Y 
Sbjct: 104 AYSKPVVFLDILWNLAFVAIGVAVLILSRDEKPNMPLRVWVVGYGIQCWLHMACVCVEYR 163

Query: 130 RRSSRYR------LGFENRGAQDELRLSHNRIR-----VMKRLEALNTMVAYIWWVFGFY 189
           RR  R         G  N  +Q  + L+    R       K LE+ NTM ++IWW+ GFY
Sbjct: 164 RRRRRRHPEDGGGSGLTNSSSQQYVSLAQLEDRGETSNPAKHLESANTMFSFIWWIIGFY 223

Query: 190 WIVMGGQALLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFAVCCIIP-FLAYGYTMN 249
           W+  GGQ L   SP+LYWL ++FL FDVFF++FC  +A V   AVCC +P  +A  Y + 
Sbjct: 224 WVSAGGQTLSSDSPQLYWLCIIFLGFDVFFVVFCVALACVIGLAVCCCLPCIIAILYAVA 283

Query: 250 FLEGASEDEIRALPKYRFHQDNPLESFDNDKKQEVGMTLEPGYNGHTTEHTLNAEDSACC 309
             EGAS+++I  +PK+RF +   +E      +   G+  E G +    E +L+ ED+ CC
Sbjct: 284 DQEGASKNDIDQMPKFRFTKTGNVEKLSGKAR---GIMTECGTDS-PIERSLSPEDAECC 343

Query: 310 ICLAQYVHGVQLCMLPCNHHFHTRCIVKWLRINATCPLCKFSI 341
           ICL +Y  GV+L  LPCNHHFH  CI KWL IN+ CPLCKF+I
Sbjct: 344 ICLCEYEDGVELRELPCNHHFHCTCIDKWLHINSRCPLCKFNI 382

BLAST of CsGy4G017820 vs. ExPASy Swiss-Prot
Match: Q8LDB8 (E3 ubiquitin-protein ligase At1g63170 OS=Arabidopsis thaliana OX=3702 GN=At1g63170 PE=2 SV=2)

HSP 1 Score: 238.0 bits (606), Expect = 1.6e-61
Identity = 139/358 (38.83%), Postives = 198/358 (55.31%), Query Frame = 0

Query: 16  RTMSSRFALPLIQDAAN--LIMEHRGVIFGDCADPRVDDDEDDNEYERRVLFRNGSSFIK 75
           R    R A  L++ A++  ++M    ++  + A  ++++ + D  Y             K
Sbjct: 25  RRQGLREAARLLRHASSGRMMMREPSMLVREAAAEQLEERQSDWAYS------------K 84

Query: 76  PILVLDLFWNLFFVFLSVIVLVLSAEEKPTAPLRFWLSGYAVQCLFHVFFVFVAYLRRSS 135
           P++VLD  WNL FV ++  VLVLS++E P  PLR W+ GY +QC+ H+  V V Y RR+S
Sbjct: 85  PVVVLDFVWNLAFVVVATAVLVLSSDENPNMPLRVWIIGYGLQCMMHMVCVCVEYRRRNS 144

Query: 136 RYRLGFENRGA------------QDELRLSHN--------------RIRVMKRLEALNTM 195
           R R     R +            ++ L LS N                   K LE+ NTM
Sbjct: 145 RRRRDLSPRSSSSSSSSSSSMDEEEGLGLSRNSDERYLELGQLENENNSFAKHLESANTM 204

Query: 196 VAYIWWVFGFYWIVMGGQALLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFAVCCII 255
           +++IWWV GFYW+  GGQ L +GSP+LYWL +VFL FDVFF++FC  +A V   AVCC +
Sbjct: 205 ISFIWWVIGFYWVSSGGQELAQGSPQLYWLCIVFLGFDVFFVVFCVALACVIGIAVCCCL 264

Query: 256 P-FLAYGYTMNFLEGASEDEIRALPKYRFHQ--DNPLESFDNDKKQ--EVGMTLEPGYNG 315
           P  +A  Y +   EGAS+++I  L K++F +  D    + D ++ Q    G+  E G + 
Sbjct: 265 PCIIAVLYAVAEQEGASKEDIDQLTKFKFRKVGDTMKHTVDEEQGQGDSGGVMTECGTDS 324

Query: 316 HTTEHTLNAEDSACCICLAQYVHGVQLCMLPCNHHFHTRCIVKWLRINATCPLCKFSI 341
              EH L  ED+ CCICL+ Y    +L  LPC HHFH  C+ KWL INATCPLCK++I
Sbjct: 325 -PVEHALPHEDAECCICLSAYEDETELRELPCGHHFHCGCVDKWLYINATCPLCKYNI 369

BLAST of CsGy4G017820 vs. ExPASy Swiss-Prot
Match: Q8GUU2 (E3 ubiquitin protein ligase RIE1 OS=Arabidopsis thaliana OX=3702 GN=RIE1 PE=2 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 4.3e-59
Identity = 122/295 (41.36%), Postives = 177/295 (60.00%), Query Frame = 0

Query: 71  FIKPILVLDLFWNLFFVFLSVIVLVLSAEEKPTAPLRFWLSGYAVQCLFHVFFVFVAYLR 130
           + KP++  D+ WN   V  S ++LV + EE+P  P+R W+  Y +QCLFHV  V+  Y R
Sbjct: 73  YSKPVVAADILWNAALVLASAVMLVGTVEERPNEPIRVWICVYGLQCLFHVVLVWSEYWR 132

Query: 131 RSSR-------------YRLGFENRGAQDELRLSHNRIRVMKRLEALNTMVAYIWWVFGF 190
           R+S              Y + ++     D+   +++    +KR E++NT++++IWW+ GF
Sbjct: 133 RNSTRRARDLESYDHEDYNIEYDYEQDSDDNSTTYS---FVKRCESINTVISFIWWIIGF 192

Query: 191 YWIVMGGQALLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFAVCCIIP-FLAYGYTM 250
           YW+V GG  LL  +P LYWL+V+FLA DVFF +FC  +A +   A+CC +P  +A  Y +
Sbjct: 193 YWVVEGGDKLLGEAPNLYWLSVIFLAIDVFFAVFCVVLACLVGIALCCCLPCIIALLYAV 252

Query: 251 NFLEGASEDEIRALPKYRFHQDNPLESFDNDKKQEVGMTLEPG------YNG--HTTEHT 310
              EG SE E+  LP Y+F      ++F +++K   G    PG       NG    TE T
Sbjct: 253 AGTEGVSEAELGVLPLYKF------KAFHSNEKNITG----PGKMVPIPINGLCLATERT 312

Query: 311 LNAEDSACCICLAQYVHGVQLCMLPCNHHFHTRCIVKWLRINATCPLCKFSIGQG 344
           L AED+ CCICL+ Y  G +L  LPCNHHFH+ CIVKWL++ ATCPLCK++I +G
Sbjct: 313 LLAEDADCCICLSSYEDGAELHALPCNHHFHSTCIVKWLKMRATCPLCKYNILKG 354

BLAST of CsGy4G017820 vs. ExPASy Swiss-Prot
Match: Q7T037 (E3 ubiquitin-protein ligase RNF12-B OS=Xenopus laevis OX=8355 GN=rnf12-b PE=2 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 1.3e-10
Identity = 37/110 (33.64%), Postives = 57/110 (51.82%), Query Frame = 0

Query: 241 GASEDEIRALPKYRFHQDNPLESFDNDKKQEVGMTLEPGYNGHTTEHTLNAEDSACCICL 300
           G + +E  +LP     Q   L   D+D  Q  G+T E   N  T  +  N     C +C+
Sbjct: 650 GVTLEESGSLPFLSLAQFFLLNEDDDD--QPRGLTKEQIDNLSTRNYGENDALKTCSVCI 709

Query: 301 AQYVHGVQLCMLPCNHHFHTRCIVKWLRINATCPLCKFSI---GQGDSLV 348
            +Y  G +L  LPC+H +H  CI +WL  N+TCP+C+ ++   G  +S+V
Sbjct: 710 TEYTEGNKLRKLPCSHEYHIHCIDRWLSENSTCPICRRAVLVAGNRESIV 757

BLAST of CsGy4G017820 vs. NCBI nr
Match: XP_004144310.1 (E3 ubiquitin-protein ligase At4g11680 [Cucumis sativus] >KGN54665.1 hypothetical protein Csa_012005 [Cucumis sativus])

HSP 1 Score: 713 bits (1840), Expect = 1.89e-259
Identity = 347/347 (100.00%), Postives = 347/347 (100.00%), Query Frame = 0

Query: 1   MDDVRPSNSGFLSWQRTMSSRFALPLIQDAANLIMEHRGVIFGDCADPRVDDDEDDNEYE 60
           MDDVRPSNSGFLSWQRTMSSRFALPLIQDAANLIMEHRGVIFGDCADPRVDDDEDDNEYE
Sbjct: 1   MDDVRPSNSGFLSWQRTMSSRFALPLIQDAANLIMEHRGVIFGDCADPRVDDDEDDNEYE 60

Query: 61  RRVLFRNGSSFIKPILVLDLFWNLFFVFLSVIVLVLSAEEKPTAPLRFWLSGYAVQCLFH 120
           RRVLFRNGSSFIKPILVLDLFWNLFFVFLSVIVLVLSAEEKPTAPLRFWLSGYAVQCLFH
Sbjct: 61  RRVLFRNGSSFIKPILVLDLFWNLFFVFLSVIVLVLSAEEKPTAPLRFWLSGYAVQCLFH 120

Query: 121 VFFVFVAYLRRSSRYRLGFENRGAQDELRLSHNRIRVMKRLEALNTMVAYIWWVFGFYWI 180
           VFFVFVAYLRRSSRYRLGFENRGAQDELRLSHNRIRVMKRLEALNTMVAYIWWVFGFYWI
Sbjct: 121 VFFVFVAYLRRSSRYRLGFENRGAQDELRLSHNRIRVMKRLEALNTMVAYIWWVFGFYWI 180

Query: 181 VMGGQALLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFAVCCIIPFLAYGYTMNFLE 240
           VMGGQALLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFAVCCIIPFLAYGYTMNFLE
Sbjct: 181 VMGGQALLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFAVCCIIPFLAYGYTMNFLE 240

Query: 241 GASEDEIRALPKYRFHQDNPLESFDNDKKQEVGMTLEPGYNGHTTEHTLNAEDSACCICL 300
           GASEDEIRALPKYRFHQDNPLESFDNDKKQEVGMTLEPGYNGHTTEHTLNAEDSACCICL
Sbjct: 241 GASEDEIRALPKYRFHQDNPLESFDNDKKQEVGMTLEPGYNGHTTEHTLNAEDSACCICL 300

Query: 301 AQYVHGVQLCMLPCNHHFHTRCIVKWLRINATCPLCKFSIGQGDSLV 347
           AQYVHGVQLCMLPCNHHFHTRCIVKWLRINATCPLCKFSIGQGDSLV
Sbjct: 301 AQYVHGVQLCMLPCNHHFHTRCIVKWLRINATCPLCKFSIGQGDSLV 347

BLAST of CsGy4G017820 vs. NCBI nr
Match: XP_038881255.1 (E3 ubiquitin-protein ligase At4g11680-like [Benincasa hispida])

HSP 1 Score: 686 bits (1769), Expect = 1.26e-248
Identity = 330/347 (95.10%), Postives = 341/347 (98.27%), Query Frame = 0

Query: 1   MDDVRPSNSGFLSWQRTMSSRFALPLIQDAANLIMEHRGVIFGDCADPRVDDDEDDNEYE 60
           MDD RPS++ FLSWQRT+SSRFALPLIQDAANLIMEHRGVIFGDCADPRVDD+EDDNEY+
Sbjct: 1   MDDGRPSSTSFLSWQRTISSRFALPLIQDAANLIMEHRGVIFGDCADPRVDDEEDDNEYD 60

Query: 61  RRVLFRNGSSFIKPILVLDLFWNLFFVFLSVIVLVLSAEEKPTAPLRFWLSGYAVQCLFH 120
           RRVLFRNGSSFIKPILVLDLFWNLFFVFLSVIVLVLSAEEKPTAPLRFWLSGYAVQCLFH
Sbjct: 61  RRVLFRNGSSFIKPILVLDLFWNLFFVFLSVIVLVLSAEEKPTAPLRFWLSGYAVQCLFH 120

Query: 121 VFFVFVAYLRRSSRYRLGFENRGAQDELRLSHNRIRVMKRLEALNTMVAYIWWVFGFYWI 180
           VFFVFVAYLRRS+RYR GFENRGAQDE RLSH+RIRVMKRLEALNTM+AYIWWVFGFYWI
Sbjct: 121 VFFVFVAYLRRSNRYRFGFENRGAQDEFRLSHHRIRVMKRLEALNTMIAYIWWVFGFYWI 180

Query: 181 VMGGQALLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFAVCCIIPFLAYGYTMNFLE 240
           VMGGQALLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFAVCCI+PFLAYGYTM FLE
Sbjct: 181 VMGGQALLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFAVCCIVPFLAYGYTMTFLE 240

Query: 241 GASEDEIRALPKYRFHQDNPLESFDNDKKQEVGMTLEPGYNGHTTEHTLNAEDSACCICL 300
           GASEDEIRALPKYRFHQDNPLESFD+DKKQEVGMTLEPGY+GHTTEHTLN EDSACCICL
Sbjct: 241 GASEDEIRALPKYRFHQDNPLESFDSDKKQEVGMTLEPGYSGHTTEHTLNPEDSACCICL 300

Query: 301 AQYVHGVQLCMLPCNHHFHTRCIVKWLRINATCPLCKFSIGQGDSLV 347
           AQYVHGVQLCMLPCNHHFHTRCIVKWLRINATCPLCKFSIGQGDSLV
Sbjct: 301 AQYVHGVQLCMLPCNHHFHTRCIVKWLRINATCPLCKFSIGQGDSLV 347

BLAST of CsGy4G017820 vs. NCBI nr
Match: XP_022154475.1 (E3 ubiquitin-protein ligase At4g11680-like [Momordica charantia])

HSP 1 Score: 651 bits (1680), Expect = 4.63e-235
Identity = 310/347 (89.34%), Postives = 333/347 (95.97%), Query Frame = 0

Query: 1   MDDVRPSNSGFLSWQRTMSSRFALPLIQDAANLIMEHRGVIFGDCADPRVDDDEDDNEYE 60
           MD+ RPS++GF+SW+RTMSSRFALPLIQDAANLIMEHRGVIFGDCADPRVDD++D+ EY+
Sbjct: 1   MDEGRPSSTGFMSWRRTMSSRFALPLIQDAANLIMEHRGVIFGDCADPRVDDEDDETEYD 60

Query: 61  RRVLFRNGSSFIKPILVLDLFWNLFFVFLSVIVLVLSAEEKPTAPLRFWLSGYAVQCLFH 120
           RRV+FR+GSSFIKPILVLDLFWNLFFVF++V+VLVLSAEEKPTAPLRFWLSGYAVQCLFH
Sbjct: 61  RRVIFRHGSSFIKPILVLDLFWNLFFVFVAVLVLVLSAEEKPTAPLRFWLSGYAVQCLFH 120

Query: 121 VFFVFVAYLRRSSRYRLGFENRGAQDELRLSHNRIRVMKRLEALNTMVAYIWWVFGFYWI 180
           VFFVF AYLRRSSRYR GFE+RGAQDELR SHNRIR MKRLEALNTMVAYIWWVFGFY I
Sbjct: 121 VFFVFFAYLRRSSRYRFGFEHRGAQDELRFSHNRIRAMKRLEALNTMVAYIWWVFGFYLI 180

Query: 181 VMGGQALLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFAVCCIIPFLAYGYTMNFLE 240
           VMGGQAL+EGSPRLYWLAV+FLAFDVFFIIFCTGMAFVG FAVCCIIPFLAYGYTM FLE
Sbjct: 181 VMGGQALVEGSPRLYWLAVIFLAFDVFFIIFCTGMAFVGLFAVCCIIPFLAYGYTMTFLE 240

Query: 241 GASEDEIRALPKYRFHQDNPLESFDNDKKQEVGMTLEPGYNGHTTEHTLNAEDSACCICL 300
           GASEDEIRALPKYRFHQDNPLE FD+DK QE+GMTLEPG+N HTTEHTL+ EDSACCICL
Sbjct: 241 GASEDEIRALPKYRFHQDNPLEPFDSDKNQEIGMTLEPGFNDHTTEHTLHPEDSACCICL 300

Query: 301 AQYVHGVQLCMLPCNHHFHTRCIVKWLRINATCPLCKFSIGQGDSLV 347
           AQYVHGVQLCMLPC HHFHTRCIVKWLRINATCPLCKF+IGQGD+LV
Sbjct: 301 AQYVHGVQLCMLPCTHHFHTRCIVKWLRINATCPLCKFTIGQGDTLV 347

BLAST of CsGy4G017820 vs. NCBI nr
Match: XP_023543467.1 (E3 ubiquitin-protein ligase At1g63170-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 639 bits (1648), Expect = 3.49e-230
Identity = 308/347 (88.76%), Postives = 325/347 (93.66%), Query Frame = 0

Query: 1   MDDVRPSNSGFLSWQRTMSSRFALPLIQDAANLIMEHRGVIFGDCADPRVDDDEDDNEYE 60
           MD  RPS++GFLSW+RT+SSRFALPLIQDAANLIMEHRGVIFGDCA+PRVDD+ED+N Y+
Sbjct: 1   MDGGRPSSTGFLSWRRTLSSRFALPLIQDAANLIMEHRGVIFGDCAEPRVDDEEDENGYD 60

Query: 61  RRVLFRNGSSFIKPILVLDLFWNLFFVFLSVIVLVLSAEEKPTAPLRFWLSGYAVQCLFH 120
           RRV  RNGSSFIKPILVLDLFWNLFFVF+SVIVLVLSAEEKP+APLR WLSGYAVQCL H
Sbjct: 61  RRVFLRNGSSFIKPILVLDLFWNLFFVFVSVIVLVLSAEEKPSAPLRLWLSGYAVQCLLH 120

Query: 121 VFFVFVAYLRRSSRYRLGFENRGAQDELRLSHNRIRVMKRLEALNTMVAYIWWVFGFYWI 180
           VFFVF AY RRSSRYR  FENR AQDELRLSHNRIR +KRLEA NTM++YIWWVFGFYWI
Sbjct: 121 VFFVFFAYFRRSSRYRFVFENRVAQDELRLSHNRIRFLKRLEASNTMISYIWWVFGFYWI 180

Query: 181 VMGGQALLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFAVCCIIPFLAYGYTMNFLE 240
           V GGQ+LLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFF VCCIIPFLAYGYTM FLE
Sbjct: 181 VTGGQSLLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFTVCCIIPFLAYGYTMTFLE 240

Query: 241 GASEDEIRALPKYRFHQDNPLESFDNDKKQEVGMTLEPGYNGHTTEHTLNAEDSACCICL 300
           GASEDEIRALPKYRF QDNPLESFD+DKK EVGMTLEPGYNG+TTEH LN EDSACCICL
Sbjct: 241 GASEDEIRALPKYRFRQDNPLESFDSDKKHEVGMTLEPGYNGYTTEHLLNTEDSACCICL 300

Query: 301 AQYVHGVQLCMLPCNHHFHTRCIVKWLRINATCPLCKFSIGQGDSLV 347
           AQYVHGVQLCMLPC+HHFHTRCIVKWLRINATCPLCKFSIG GDSLV
Sbjct: 301 AQYVHGVQLCMLPCSHHFHTRCIVKWLRINATCPLCKFSIGHGDSLV 347

BLAST of CsGy4G017820 vs. NCBI nr
Match: XP_022949865.1 (E3 ubiquitin-protein ligase At1g63170-like [Cucurbita moschata] >KAG6603427.1 E3 ubiquitin protein ligase RIE1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 639 bits (1647), Expect = 4.95e-230
Identity = 307/347 (88.47%), Postives = 325/347 (93.66%), Query Frame = 0

Query: 1   MDDVRPSNSGFLSWQRTMSSRFALPLIQDAANLIMEHRGVIFGDCADPRVDDDEDDNEYE 60
           MD  RPS++GFLSW+RT+SSRFALPLIQDAANLIMEHRGVIFGDCA+PRVDD+ED+N Y+
Sbjct: 1   MDGGRPSSTGFLSWRRTLSSRFALPLIQDAANLIMEHRGVIFGDCAEPRVDDEEDENGYD 60

Query: 61  RRVLFRNGSSFIKPILVLDLFWNLFFVFLSVIVLVLSAEEKPTAPLRFWLSGYAVQCLFH 120
           RRV  RNGSSFIKPILVLDLFWNLFFVF+SVIVLVLSAEEKP+APLR WLSGYAVQCL H
Sbjct: 61  RRVFLRNGSSFIKPILVLDLFWNLFFVFVSVIVLVLSAEEKPSAPLRLWLSGYAVQCLLH 120

Query: 121 VFFVFVAYLRRSSRYRLGFENRGAQDELRLSHNRIRVMKRLEALNTMVAYIWWVFGFYWI 180
           VFFVF AY RRSSRYR  FENR AQDELRLSHNRIR +KRLEA NTM++YIWWVFGFYWI
Sbjct: 121 VFFVFFAYFRRSSRYRFVFENRVAQDELRLSHNRIRFLKRLEASNTMISYIWWVFGFYWI 180

Query: 181 VMGGQALLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFAVCCIIPFLAYGYTMNFLE 240
           V GGQ+LLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFF VCCIIPFLAYGYTM FLE
Sbjct: 181 VTGGQSLLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFTVCCIIPFLAYGYTMTFLE 240

Query: 241 GASEDEIRALPKYRFHQDNPLESFDNDKKQEVGMTLEPGYNGHTTEHTLNAEDSACCICL 300
           GASEDEIRALPKYRF QDNPLESFD+DKK EVGMTLEPGYNG+TTEH LN EDSACCICL
Sbjct: 241 GASEDEIRALPKYRFRQDNPLESFDSDKKHEVGMTLEPGYNGYTTEHLLNTEDSACCICL 300

Query: 301 AQYVHGVQLCMLPCNHHFHTRCIVKWLRINATCPLCKFSIGQGDSLV 347
           AQYVHGVQLCMLPC+HHFHTRCIVKWLRINATCPLCKFS+G GDSLV
Sbjct: 301 AQYVHGVQLCMLPCSHHFHTRCIVKWLRINATCPLCKFSVGHGDSLV 347

BLAST of CsGy4G017820 vs. ExPASy TrEMBL
Match: A0A0A0KYD1 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G418580 PE=4 SV=1)

HSP 1 Score: 713 bits (1840), Expect = 9.14e-260
Identity = 347/347 (100.00%), Postives = 347/347 (100.00%), Query Frame = 0

Query: 1   MDDVRPSNSGFLSWQRTMSSRFALPLIQDAANLIMEHRGVIFGDCADPRVDDDEDDNEYE 60
           MDDVRPSNSGFLSWQRTMSSRFALPLIQDAANLIMEHRGVIFGDCADPRVDDDEDDNEYE
Sbjct: 1   MDDVRPSNSGFLSWQRTMSSRFALPLIQDAANLIMEHRGVIFGDCADPRVDDDEDDNEYE 60

Query: 61  RRVLFRNGSSFIKPILVLDLFWNLFFVFLSVIVLVLSAEEKPTAPLRFWLSGYAVQCLFH 120
           RRVLFRNGSSFIKPILVLDLFWNLFFVFLSVIVLVLSAEEKPTAPLRFWLSGYAVQCLFH
Sbjct: 61  RRVLFRNGSSFIKPILVLDLFWNLFFVFLSVIVLVLSAEEKPTAPLRFWLSGYAVQCLFH 120

Query: 121 VFFVFVAYLRRSSRYRLGFENRGAQDELRLSHNRIRVMKRLEALNTMVAYIWWVFGFYWI 180
           VFFVFVAYLRRSSRYRLGFENRGAQDELRLSHNRIRVMKRLEALNTMVAYIWWVFGFYWI
Sbjct: 121 VFFVFVAYLRRSSRYRLGFENRGAQDELRLSHNRIRVMKRLEALNTMVAYIWWVFGFYWI 180

Query: 181 VMGGQALLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFAVCCIIPFLAYGYTMNFLE 240
           VMGGQALLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFAVCCIIPFLAYGYTMNFLE
Sbjct: 181 VMGGQALLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFAVCCIIPFLAYGYTMNFLE 240

Query: 241 GASEDEIRALPKYRFHQDNPLESFDNDKKQEVGMTLEPGYNGHTTEHTLNAEDSACCICL 300
           GASEDEIRALPKYRFHQDNPLESFDNDKKQEVGMTLEPGYNGHTTEHTLNAEDSACCICL
Sbjct: 241 GASEDEIRALPKYRFHQDNPLESFDNDKKQEVGMTLEPGYNGHTTEHTLNAEDSACCICL 300

Query: 301 AQYVHGVQLCMLPCNHHFHTRCIVKWLRINATCPLCKFSIGQGDSLV 347
           AQYVHGVQLCMLPCNHHFHTRCIVKWLRINATCPLCKFSIGQGDSLV
Sbjct: 301 AQYVHGVQLCMLPCNHHFHTRCIVKWLRINATCPLCKFSIGQGDSLV 347

BLAST of CsGy4G017820 vs. ExPASy TrEMBL
Match: A0A6J1DJQ2 (E3 ubiquitin-protein ligase At4g11680-like OS=Momordica charantia OX=3673 GN=LOC111021746 PE=4 SV=1)

HSP 1 Score: 651 bits (1680), Expect = 2.24e-235
Identity = 310/347 (89.34%), Postives = 333/347 (95.97%), Query Frame = 0

Query: 1   MDDVRPSNSGFLSWQRTMSSRFALPLIQDAANLIMEHRGVIFGDCADPRVDDDEDDNEYE 60
           MD+ RPS++GF+SW+RTMSSRFALPLIQDAANLIMEHRGVIFGDCADPRVDD++D+ EY+
Sbjct: 1   MDEGRPSSTGFMSWRRTMSSRFALPLIQDAANLIMEHRGVIFGDCADPRVDDEDDETEYD 60

Query: 61  RRVLFRNGSSFIKPILVLDLFWNLFFVFLSVIVLVLSAEEKPTAPLRFWLSGYAVQCLFH 120
           RRV+FR+GSSFIKPILVLDLFWNLFFVF++V+VLVLSAEEKPTAPLRFWLSGYAVQCLFH
Sbjct: 61  RRVIFRHGSSFIKPILVLDLFWNLFFVFVAVLVLVLSAEEKPTAPLRFWLSGYAVQCLFH 120

Query: 121 VFFVFVAYLRRSSRYRLGFENRGAQDELRLSHNRIRVMKRLEALNTMVAYIWWVFGFYWI 180
           VFFVF AYLRRSSRYR GFE+RGAQDELR SHNRIR MKRLEALNTMVAYIWWVFGFY I
Sbjct: 121 VFFVFFAYLRRSSRYRFGFEHRGAQDELRFSHNRIRAMKRLEALNTMVAYIWWVFGFYLI 180

Query: 181 VMGGQALLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFAVCCIIPFLAYGYTMNFLE 240
           VMGGQAL+EGSPRLYWLAV+FLAFDVFFIIFCTGMAFVG FAVCCIIPFLAYGYTM FLE
Sbjct: 181 VMGGQALVEGSPRLYWLAVIFLAFDVFFIIFCTGMAFVGLFAVCCIIPFLAYGYTMTFLE 240

Query: 241 GASEDEIRALPKYRFHQDNPLESFDNDKKQEVGMTLEPGYNGHTTEHTLNAEDSACCICL 300
           GASEDEIRALPKYRFHQDNPLE FD+DK QE+GMTLEPG+N HTTEHTL+ EDSACCICL
Sbjct: 241 GASEDEIRALPKYRFHQDNPLEPFDSDKNQEIGMTLEPGFNDHTTEHTLHPEDSACCICL 300

Query: 301 AQYVHGVQLCMLPCNHHFHTRCIVKWLRINATCPLCKFSIGQGDSLV 347
           AQYVHGVQLCMLPC HHFHTRCIVKWLRINATCPLCKF+IGQGD+LV
Sbjct: 301 AQYVHGVQLCMLPCTHHFHTRCIVKWLRINATCPLCKFTIGQGDTLV 347

BLAST of CsGy4G017820 vs. ExPASy TrEMBL
Match: A0A6J1GE01 (E3 ubiquitin-protein ligase At1g63170-like OS=Cucurbita moschata OX=3662 GN=LOC111453131 PE=4 SV=1)

HSP 1 Score: 639 bits (1647), Expect = 2.40e-230
Identity = 307/347 (88.47%), Postives = 325/347 (93.66%), Query Frame = 0

Query: 1   MDDVRPSNSGFLSWQRTMSSRFALPLIQDAANLIMEHRGVIFGDCADPRVDDDEDDNEYE 60
           MD  RPS++GFLSW+RT+SSRFALPLIQDAANLIMEHRGVIFGDCA+PRVDD+ED+N Y+
Sbjct: 1   MDGGRPSSTGFLSWRRTLSSRFALPLIQDAANLIMEHRGVIFGDCAEPRVDDEEDENGYD 60

Query: 61  RRVLFRNGSSFIKPILVLDLFWNLFFVFLSVIVLVLSAEEKPTAPLRFWLSGYAVQCLFH 120
           RRV  RNGSSFIKPILVLDLFWNLFFVF+SVIVLVLSAEEKP+APLR WLSGYAVQCL H
Sbjct: 61  RRVFLRNGSSFIKPILVLDLFWNLFFVFVSVIVLVLSAEEKPSAPLRLWLSGYAVQCLLH 120

Query: 121 VFFVFVAYLRRSSRYRLGFENRGAQDELRLSHNRIRVMKRLEALNTMVAYIWWVFGFYWI 180
           VFFVF AY RRSSRYR  FENR AQDELRLSHNRIR +KRLEA NTM++YIWWVFGFYWI
Sbjct: 121 VFFVFFAYFRRSSRYRFVFENRVAQDELRLSHNRIRFLKRLEASNTMISYIWWVFGFYWI 180

Query: 181 VMGGQALLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFAVCCIIPFLAYGYTMNFLE 240
           V GGQ+LLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFF VCCIIPFLAYGYTM FLE
Sbjct: 181 VTGGQSLLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFTVCCIIPFLAYGYTMTFLE 240

Query: 241 GASEDEIRALPKYRFHQDNPLESFDNDKKQEVGMTLEPGYNGHTTEHTLNAEDSACCICL 300
           GASEDEIRALPKYRF QDNPLESFD+DKK EVGMTLEPGYNG+TTEH LN EDSACCICL
Sbjct: 241 GASEDEIRALPKYRFRQDNPLESFDSDKKHEVGMTLEPGYNGYTTEHLLNTEDSACCICL 300

Query: 301 AQYVHGVQLCMLPCNHHFHTRCIVKWLRINATCPLCKFSIGQGDSLV 347
           AQYVHGVQLCMLPC+HHFHTRCIVKWLRINATCPLCKFS+G GDSLV
Sbjct: 301 AQYVHGVQLCMLPCSHHFHTRCIVKWLRINATCPLCKFSVGHGDSLV 347

BLAST of CsGy4G017820 vs. ExPASy TrEMBL
Match: A0A6J1HW81 (E3 ubiquitin-protein ligase At1g63170-like OS=Cucurbita maxima OX=3661 GN=LOC111467225 PE=4 SV=1)

HSP 1 Score: 638 bits (1646), Expect = 3.41e-230
Identity = 307/347 (88.47%), Postives = 325/347 (93.66%), Query Frame = 0

Query: 1   MDDVRPSNSGFLSWQRTMSSRFALPLIQDAANLIMEHRGVIFGDCADPRVDDDEDDNEYE 60
           MD  RP ++GFLSW+RT+SSRFALPLIQDAANLIMEHRGVIFGDCA+PRVDD+ED+N Y+
Sbjct: 1   MDGGRPLSTGFLSWRRTLSSRFALPLIQDAANLIMEHRGVIFGDCAEPRVDDEEDENGYD 60

Query: 61  RRVLFRNGSSFIKPILVLDLFWNLFFVFLSVIVLVLSAEEKPTAPLRFWLSGYAVQCLFH 120
           RRV  RNGSSFIKPILVLDLFWNLFFVF+SVIVLVLSAEEKP+APLR WLSGYAVQCL H
Sbjct: 61  RRVFLRNGSSFIKPILVLDLFWNLFFVFVSVIVLVLSAEEKPSAPLRLWLSGYAVQCLLH 120

Query: 121 VFFVFVAYLRRSSRYRLGFENRGAQDELRLSHNRIRVMKRLEALNTMVAYIWWVFGFYWI 180
           VFFVF AY RRSSRYR  FENR AQDELRLSHNRIR +KRLEA NTM++YIWWVFGFYWI
Sbjct: 121 VFFVFFAYFRRSSRYRFVFENRVAQDELRLSHNRIRFLKRLEASNTMISYIWWVFGFYWI 180

Query: 181 VMGGQALLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFAVCCIIPFLAYGYTMNFLE 240
           VMGGQ+LLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFF VCCIIPFLAYGYTM FLE
Sbjct: 181 VMGGQSLLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFTVCCIIPFLAYGYTMTFLE 240

Query: 241 GASEDEIRALPKYRFHQDNPLESFDNDKKQEVGMTLEPGYNGHTTEHTLNAEDSACCICL 300
           GASEDEIRALPKYRF QDNPLESFD+DKK EVGMTLEPGYNG+TTEH LN EDSACCICL
Sbjct: 241 GASEDEIRALPKYRFRQDNPLESFDSDKKHEVGMTLEPGYNGYTTEHLLNPEDSACCICL 300

Query: 301 AQYVHGVQLCMLPCNHHFHTRCIVKWLRINATCPLCKFSIGQGDSLV 347
           AQYVHGVQLCMLPC+HHFHTRCIVKWLRINATCPLCKFS+G GDSLV
Sbjct: 301 AQYVHGVQLCMLPCSHHFHTRCIVKWLRINATCPLCKFSVGHGDSLV 347

BLAST of CsGy4G017820 vs. ExPASy TrEMBL
Match: A0A5A7SMU7 (E3 ubiquitin-protein ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold447G00840 PE=4 SV=1)

HSP 1 Score: 624 bits (1610), Expect = 7.74e-225
Identity = 308/313 (98.40%), Postives = 310/313 (99.04%), Query Frame = 0

Query: 1   MDDVRPSNSGFLSWQRTMSSRFALPLIQDAANLIMEHRGVIFGDCADPRVDDDEDDNEYE 60
           MDDVRPSNS FLSWQRTMSSRFALPLIQDAANLIMEHRGVIFGDCADPRVDD+EDDNEY+
Sbjct: 1   MDDVRPSNSAFLSWQRTMSSRFALPLIQDAANLIMEHRGVIFGDCADPRVDDEEDDNEYD 60

Query: 61  RRVLFRNGSSFIKPILVLDLFWNLFFVFLSVIVLVLSAEEKPTAPLRFWLSGYAVQCLFH 120
           RRVLFRNGSSFIKPILVLDLFWNLFFVFLSV VLVLSAEEKPTAPLRFWLSGYAVQCLFH
Sbjct: 61  RRVLFRNGSSFIKPILVLDLFWNLFFVFLSVTVLVLSAEEKPTAPLRFWLSGYAVQCLFH 120

Query: 121 VFFVFVAYLRRSSRYRLGFENRGAQDELRLSHNRIRVMKRLEALNTMVAYIWWVFGFYWI 180
           VFFVFVAYLRRSSRYRLGFENRGAQDELRLSHNRIRVMKRLEALNTMVAYIWWVFGFYWI
Sbjct: 121 VFFVFVAYLRRSSRYRLGFENRGAQDELRLSHNRIRVMKRLEALNTMVAYIWWVFGFYWI 180

Query: 181 VMGGQALLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFAVCCIIPFLAYGYTMNFLE 240
           VMGGQALLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVG FAVCCIIPFLAYGYTMNFLE
Sbjct: 181 VMGGQALLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGLFAVCCIIPFLAYGYTMNFLE 240

Query: 241 GASEDEIRALPKYRFHQDNPLESFDNDKKQEVGMTLEPGYNGHTTEHTLNAEDSACCICL 300
           GASEDEIRALPKYRFHQDNPLESFDNDKKQEVGMTLEPGYNGHTTEHTLNAEDSACCICL
Sbjct: 241 GASEDEIRALPKYRFHQDNPLESFDNDKKQEVGMTLEPGYNGHTTEHTLNAEDSACCICL 300

Query: 301 AQYVHGVQLCMLP 313
           AQYVHGVQLCMLP
Sbjct: 301 AQYVHGVQLCMLP 313

BLAST of CsGy4G017820 vs. TAIR 10
Match: AT1G68070.1 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 252.7 bits (644), Expect = 4.4e-67
Identity = 125/285 (43.86%), Postives = 183/285 (64.21%), Query Frame = 0

Query: 71  FIKPILVLDLFWNLFFVFLSVIVLVLSAEEKPTAPLRFWLSGYAVQCLFHVFFVFVAYLR 130
           + KP++ LD+ WN  FV +++++L++  EEKP  P+R W+ GYA+QCL HV  V++ + +
Sbjct: 64  YSKPVVALDMLWNTAFVLVAIVMLLVFKEEKPNVPIRIWICGYAIQCLVHVVLVWLEFRK 123

Query: 131 RSSRYRLG------FENRGAQDELRLSHNRIRVMKRLEALNTMVAYIWWVFGFYWIVMGG 190
           R++R R G        N+ ++DE   +  R    K  E++NT+++++WW+ GFYW+V GG
Sbjct: 124 RNARSRPGDLEAAQATNQDSEDE--DNDERFLSTKTCESMNTIISFVWWIVGFYWLVSGG 183

Query: 191 QALLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFAVCCIIP-FLAYGYTMNFLEGAS 250
             LL+ +  LYWL  VFLAFDVFF IFC  +A +   A+CC +P  +A  Y +   EGAS
Sbjct: 184 DILLQNATHLYWLTFVFLAFDVFFAIFCVVLACLIGIALCCCLPCIIALLYAVAGQEGAS 243

Query: 251 EDEIRALPKYRFHQDNPLESFDNDKKQEVG----MTLEPGYNGHTTEHTLNAEDSACCIC 310
           E ++  LPKYRFH      + +ND+KQ  G    + ++        E  L  ED+ CCIC
Sbjct: 244 EADLSILPKYRFH------TMNNDEKQSDGGGKMIPVDAASENLGNERVLLPEDADCCIC 303

Query: 311 LAQYVHGVQLCMLPCNHHFHTRCIVKWLRINATCPLCKFSIGQGD 345
           L+ Y  G +L  LPCNHHFH+ CIVKWL++NATCPLCKF+I +G+
Sbjct: 304 LSSYEDGAELVSLPCNHHFHSTCIVKWLKMNATCPLCKFNILKGN 340

BLAST of CsGy4G017820 vs. TAIR 10
Match: AT1G12760.1 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 241.1 bits (614), Expect = 1.3e-63
Identity = 130/310 (41.94%), Postives = 177/310 (57.10%), Query Frame = 0

Query: 70  SFIKPILVLDLFWNLFFVFLSVIVLVLSAEEKPTAPLRFWLSGYAVQCLFHVFFVFVAYL 129
           ++ KP++VLD+ WNL FV ++  +LV+S +E P  PLR WL GYA+QC+ H+  V V Y 
Sbjct: 95  AYSKPVVVLDIVWNLAFVSVATAILVMSRKEHPIMPLRVWLLGYALQCVLHMVCVCVEYR 154

Query: 130 RRSSR---------------------------YRLGFENRGAQDELRLSH---NRIRVMK 189
           RR+ R                             LG        +L L H       V K
Sbjct: 155 RRNRRRTNRTTTTTPPRSRSSSSSSSSSSLEEEALGSRRNSGVQDLSLGHLDTESSSVAK 214

Query: 190 RLEALNTMVAYIWWVFGFYWIVMGGQALLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVG 249
            LE+ NTM ++IWW+ GFYW+  GGQ L + SPR+YWL++VFL FDVFF++FC  +A V 
Sbjct: 215 HLESANTMFSFIWWIIGFYWVSAGGQELAQESPRIYWLSIVFLGFDVFFVVFCVALACVI 274

Query: 250 FFAVCCIIP-FLAYGYTMNFLEGASEDEIRALPKYRFHQDNPLESFDNDKKQEV--GMTL 309
             AVCC +P  +A  Y +   EGAS+++I  L K++F +        ND+ Q    G+  
Sbjct: 275 GIAVCCCLPCIIAVLYAVADQEGASKEDIEQLTKFKFRKLGDANKHTNDEAQGTTEGIMT 334

Query: 310 EPGYNGHTTEHTLNAEDSACCICLAQYVHGVQLCMLPCNHHFHTRCIVKWLRINATCPLC 347
           E G +    EHTL  ED+ CCICL+ Y  G +L  LPC HHFH  C+ KWL INATCPLC
Sbjct: 335 ECGTDS-PIEHTLLQEDAECCICLSAYEDGTELRELPCGHHFHCSCVDKWLYINATCPLC 394

BLAST of CsGy4G017820 vs. TAIR 10
Match: AT1G12760.2 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 241.1 bits (614), Expect = 1.3e-63
Identity = 130/310 (41.94%), Postives = 177/310 (57.10%), Query Frame = 0

Query: 70  SFIKPILVLDLFWNLFFVFLSVIVLVLSAEEKPTAPLRFWLSGYAVQCLFHVFFVFVAYL 129
           ++ KP++VLD+ WNL FV ++  +LV+S +E P  PLR WL GYA+QC+ H+  V V Y 
Sbjct: 24  AYSKPVVVLDIVWNLAFVSVATAILVMSRKEHPIMPLRVWLLGYALQCVLHMVCVCVEYR 83

Query: 130 RRSSR---------------------------YRLGFENRGAQDELRLSH---NRIRVMK 189
           RR+ R                             LG        +L L H       V K
Sbjct: 84  RRNRRRTNRTTTTTPPRSRSSSSSSSSSSLEEEALGSRRNSGVQDLSLGHLDTESSSVAK 143

Query: 190 RLEALNTMVAYIWWVFGFYWIVMGGQALLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVG 249
            LE+ NTM ++IWW+ GFYW+  GGQ L + SPR+YWL++VFL FDVFF++FC  +A V 
Sbjct: 144 HLESANTMFSFIWWIIGFYWVSAGGQELAQESPRIYWLSIVFLGFDVFFVVFCVALACVI 203

Query: 250 FFAVCCIIP-FLAYGYTMNFLEGASEDEIRALPKYRFHQDNPLESFDNDKKQEV--GMTL 309
             AVCC +P  +A  Y +   EGAS+++I  L K++F +        ND+ Q    G+  
Sbjct: 204 GIAVCCCLPCIIAVLYAVADQEGASKEDIEQLTKFKFRKLGDANKHTNDEAQGTTEGIMT 263

Query: 310 EPGYNGHTTEHTLNAEDSACCICLAQYVHGVQLCMLPCNHHFHTRCIVKWLRINATCPLC 347
           E G +    EHTL  ED+ CCICL+ Y  G +L  LPC HHFH  C+ KWL INATCPLC
Sbjct: 264 ECGTDS-PIEHTLLQEDAECCICLSAYEDGTELRELPCGHHFHCSCVDKWLYINATCPLC 323

BLAST of CsGy4G017820 vs. TAIR 10
Match: AT4G11680.1 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 241.1 bits (614), Expect = 1.3e-63
Identity = 126/283 (44.52%), Postives = 171/283 (60.42%), Query Frame = 0

Query: 70  SFIKPILVLDLFWNLFFVFLSVIVLVLSAEEKPTAPLRFWLSGYAVQCLFHVFFVFVAYL 129
           ++ KP++ LD+ WNL FV + V VL+LS +EKP  PLR W+ GY +QC  H+  V V Y 
Sbjct: 104 AYSKPVVFLDILWNLAFVAIGVAVLILSRDEKPNMPLRVWVVGYGIQCWLHMACVCVEYR 163

Query: 130 RRSSRYR------LGFENRGAQDELRLSHNRIR-----VMKRLEALNTMVAYIWWVFGFY 189
           RR  R         G  N  +Q  + L+    R       K LE+ NTM ++IWW+ GFY
Sbjct: 164 RRRRRRHPEDGGGSGLTNSSSQQYVSLAQLEDRGETSNPAKHLESANTMFSFIWWIIGFY 223

Query: 190 WIVMGGQALLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFAVCCIIP-FLAYGYTMN 249
           W+  GGQ L   SP+LYWL ++FL FDVFF++FC  +A V   AVCC +P  +A  Y + 
Sbjct: 224 WVSAGGQTLSSDSPQLYWLCIIFLGFDVFFVVFCVALACVIGLAVCCCLPCIIAILYAVA 283

Query: 250 FLEGASEDEIRALPKYRFHQDNPLESFDNDKKQEVGMTLEPGYNGHTTEHTLNAEDSACC 309
             EGAS+++I  +PK+RF +   +E      +   G+  E G +    E +L+ ED+ CC
Sbjct: 284 DQEGASKNDIDQMPKFRFTKTGNVEKLSGKAR---GIMTECGTDS-PIERSLSPEDAECC 343

Query: 310 ICLAQYVHGVQLCMLPCNHHFHTRCIVKWLRINATCPLCKFSI 341
           ICL +Y  GV+L  LPCNHHFH  CI KWL IN+ CPLCKF+I
Sbjct: 344 ICLCEYEDGVELRELPCNHHFHCTCIDKWLHINSRCPLCKFNI 382

BLAST of CsGy4G017820 vs. TAIR 10
Match: AT1G63170.1 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 238.0 bits (606), Expect = 1.1e-62
Identity = 139/358 (38.83%), Postives = 198/358 (55.31%), Query Frame = 0

Query: 16  RTMSSRFALPLIQDAAN--LIMEHRGVIFGDCADPRVDDDEDDNEYERRVLFRNGSSFIK 75
           R    R A  L++ A++  ++M    ++  + A  ++++ + D  Y             K
Sbjct: 25  RRQGLREAARLLRHASSGRMMMREPSMLVREAAAEQLEERQSDWAYS------------K 84

Query: 76  PILVLDLFWNLFFVFLSVIVLVLSAEEKPTAPLRFWLSGYAVQCLFHVFFVFVAYLRRSS 135
           P++VLD  WNL FV ++  VLVLS++E P  PLR W+ GY +QC+ H+  V V Y RR+S
Sbjct: 85  PVVVLDFVWNLAFVVVATAVLVLSSDENPNMPLRVWIIGYGLQCMMHMVCVCVEYRRRNS 144

Query: 136 RYRLGFENRGA------------QDELRLSHN--------------RIRVMKRLEALNTM 195
           R R     R +            ++ L LS N                   K LE+ NTM
Sbjct: 145 RRRRDLSPRSSSSSSSSSSSMDEEEGLGLSRNSDERYLELGQLENENNSFAKHLESANTM 204

Query: 196 VAYIWWVFGFYWIVMGGQALLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFAVCCII 255
           +++IWWV GFYW+  GGQ L +GSP+LYWL +VFL FDVFF++FC  +A V   AVCC +
Sbjct: 205 ISFIWWVIGFYWVSSGGQELAQGSPQLYWLCIVFLGFDVFFVVFCVALACVIGIAVCCCL 264

Query: 256 P-FLAYGYTMNFLEGASEDEIRALPKYRFHQ--DNPLESFDNDKKQ--EVGMTLEPGYNG 315
           P  +A  Y +   EGAS+++I  L K++F +  D    + D ++ Q    G+  E G + 
Sbjct: 265 PCIIAVLYAVAEQEGASKEDIDQLTKFKFRKVGDTMKHTVDEEQGQGDSGGVMTECGTDS 324

Query: 316 HTTEHTLNAEDSACCICLAQYVHGVQLCMLPCNHHFHTRCIVKWLRINATCPLCKFSI 341
              EH L  ED+ CCICL+ Y    +L  LPC HHFH  C+ KWL INATCPLCK++I
Sbjct: 325 -PVEHALPHEDAECCICLSAYEDETELRELPCGHHFHCGCVDKWLYINATCPLCKYNI 369

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LN711.8e-6241.94E3 ubiquitin-protein ligase At1g12760 OS=Arabidopsis thaliana OX=3702 GN=At1g127... [more]
Q93Z921.8e-6244.52E3 ubiquitin-protein ligase At4g11680 OS=Arabidopsis thaliana OX=3702 GN=At4g116... [more]
Q8LDB81.6e-6138.83E3 ubiquitin-protein ligase At1g63170 OS=Arabidopsis thaliana OX=3702 GN=At1g631... [more]
Q8GUU24.3e-5941.36E3 ubiquitin protein ligase RIE1 OS=Arabidopsis thaliana OX=3702 GN=RIE1 PE=2 SV... [more]
Q7T0371.3e-1033.64E3 ubiquitin-protein ligase RNF12-B OS=Xenopus laevis OX=8355 GN=rnf12-b PE=2 SV... [more]
Match NameE-valueIdentityDescription
XP_004144310.11.89e-259100.00E3 ubiquitin-protein ligase At4g11680 [Cucumis sativus] >KGN54665.1 hypothetical... [more]
XP_038881255.11.26e-24895.10E3 ubiquitin-protein ligase At4g11680-like [Benincasa hispida][more]
XP_022154475.14.63e-23589.34E3 ubiquitin-protein ligase At4g11680-like [Momordica charantia][more]
XP_023543467.13.49e-23088.76E3 ubiquitin-protein ligase At1g63170-like [Cucurbita pepo subsp. pepo][more]
XP_022949865.14.95e-23088.47E3 ubiquitin-protein ligase At1g63170-like [Cucurbita moschata] >KAG6603427.1 E3... [more]
Match NameE-valueIdentityDescription
A0A0A0KYD19.14e-260100.00RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G418580 P... [more]
A0A6J1DJQ22.24e-23589.34E3 ubiquitin-protein ligase At4g11680-like OS=Momordica charantia OX=3673 GN=LOC... [more]
A0A6J1GE012.40e-23088.47E3 ubiquitin-protein ligase At1g63170-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1HW813.41e-23088.47E3 ubiquitin-protein ligase At1g63170-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A5A7SMU77.74e-22598.40E3 ubiquitin-protein ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
Match NameE-valueIdentityDescription
AT1G68070.14.4e-6743.86Zinc finger, C3HC4 type (RING finger) family protein [more]
AT1G12760.11.3e-6341.94Zinc finger, C3HC4 type (RING finger) family protein [more]
AT1G12760.21.3e-6341.94Zinc finger, C3HC4 type (RING finger) family protein [more]
AT4G11680.11.3e-6344.52Zinc finger, C3HC4 type (RING finger) family protein [more]
AT1G63170.11.1e-6238.83Zinc finger, C3HC4 type (RING finger) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 296..336
e-value: 2.4E-5
score: 33.7
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 295..337
e-value: 1.3E-10
score: 41.5
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 296..337
score: 12.297489
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 277..344
e-value: 3.3E-17
score: 63.5
NoneNo IPR availablePANTHERPTHR45977TARGET OF ERK KINASE MPK-1coord: 12..347
NoneNo IPR availablePANTHERPTHR45977:SF19RING-FINGER UBIQUITIN LIGASEcoord: 12..347
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 282..341

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy4G017820.2CsGy4G017820.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane