CsGy4G017590 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy4G017590
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionCytochrome P450
LocationGy14Chr4: 22698221 .. 22700963 (+)
RNA-Seq ExpressionCsGy4G017590
SyntenyCsGy4G017590
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCTTAGCCATGGTGATTGTTGGTCTGTTTTTACTCCTCTGTTTTTGCGCTGCTTTACTCCGACGGAATGAAGTTCGATACATAAAAAATGGATTGCCTCCTGGTACAATGGGCTGGCCTGTTTTTGGTGAAACCTCTGAGTTTCTTAAACAAGGTCCGAATTTTATGAATTCTAAAAGAGCAAGGTACTGATTAAAAATTTACTCTGTCTTTCCTTTTTCTTTTTTATTTTCTTTTGCCTTTAGATTTCTTCGTAATCTCTGGTTTTAACGTTTGCTGTCTTTTGTGAAGGTATGGGAGCTTTTTCAAATCACATATTTTGGGATCTCCTACGGTTGTTTCTATGGATCCAGAGGTTAATAGATATATTTTAATGAATGAATCTAAAGGACTCGTCGCTGGTTATCCTCAATCTATGCTTGATATACTTGGAAAATGCAATATCGCTGCCGTACACGGCGACGCTCATAAGCTTATGAGAGGTGCTCTACTTGCTATTGTTAGTCCCACCATGATTAAAGATCGTCTTCTTCTTCAAGTTGATGAGTTTATGAGATCCCATTTGCGTAATTGGGATAGCCAAATCATTGATATTCAAGAAAAAACTAAACAGGTGTATTTTAGTTTTTTAAAATACTGCAAAAATTTTCTCTGTTTTCACTCATTTTTAACGTTGTCCTCCCTCCCCCTCTCTTCAGATGGCTTTCTGGACATCTTTGAAGCAGACTACCAGTATCGAATCCGGTCCATTATGGGAATCTTTCACCATAGAATTCTTTAAGCTCGTCCTGGGCACTTTCTCGCTCCCAATCGATCTTCCAGGCACGAATTACCGTATAGGAGTTCAGGCAAGGAATAACATAGTAAAGCTATTGAGGCAGCTGATTAATGAGAGAGGAAATTCAAAGATTCAAAAAGATGACATGCTTGGATATTTATTGAGTGAAGAGAACAAATATAAACTAAATGAGGAACAGATTATTGATCAAGTCATTACTGTTTTCTATTCTGGTTATGAAACTGTTTCAACAACTTCAATGATGGCTGTAAAGTTCCTTCATGATCATCCCAAAGTTCTTCAGCAACTCAGAGTGAGTATTAAAATAAGAAAAAAAAATTTATAACTCATTGAGTTGGAAATTTGAAAAAGCTGTTGGTTCTTTTTAAAAAAATTCATTTCGAAGTTAAGAGATTTGAGATTTGCAGGAAGAACATTTGGCATTAAGAGAGAAGAAAAATCCCGAGGACCCGATCGATTGGGATGATTTTAAAGCAATGGAGTTCACTCGTGCGGTATGATTTTTTATGTTTCTACTGTCATCTTTTTAACAGTTCTTTGATGGGTTTCTTTAAAAAAAATTATCAAGATTCTAACTTTACACTGTTTTGTTATGGAATTGTTTAGGTGATTTTTGAAACATCAAGGCTAGCTACTGTTGTTAATGGAGTTTTGAGGAAAACTACAAAGGATTTGGAAGTAAATGGTGAGTTTATTAGTACTTTTTTTGTTTAAAATTTGATGATCTTATTTAAGATTAGAAAAATCATTTCTTCATTTAAACTTTCATTATGCGTCACCTACCACGCCATTGTCATTTTATGGAATTTCTACTTCAAAAGTATTTTGGTAACAAATTTAAACATTGAAAGGATAATGGTTCAAGGGTTTTGGTATGTATATAAAAGTAATTTTAAAAGTATCAGTCAGTTTTCAAACAAGCTGCAAAGTTGCATGGTAGGCAAGTTTTACTTTAGAGTTATTTGCAGTAACCAATATTCATCTCCCCAAATCACTTCAAGGCTGGTTCCTGTTTTTGCAGTTACCAATATTCATCTCCCCAATTAACTTTGGGCCACTTTTTCTTTCTTTCTCTCTCTTCTCTGTTTTTTCTTTTGAGTCAGCCTTAGGCTCATAAATAATGACGGAACTTCTTCACTCCTTTGGTTACAAGTGTTGTTTTCTATAAAATGAATCCAAACCTATATATAACCAACGAAACATACACTTTTGAAATAATATCATAATAATATCTAAAAATAATCTAATTACATAAACAACAACAATGTGGTGAGATCAAACAAAAAAGTTTCGATCTTTAACTGTTTTATAAAATAAAATTATTTTACTTTTTACAGGGTTTCTAATTCCTAAGGGATGGAGGATTTATGTGTATAATCGAGAGATAAACTATGACCCTTTTCTCTATTCTGAACCATTCACTTTCAACCCTTGGAGATGGCTGGTGGATCTCTCTCTCTCTAACTTTCTCTCTCTCTCTCTTACCTTTGTTGAAAAATGACCATTTTGCTCTTGTTTAACATTTTTCAGGACAAAAGCTTAGAGTCCCATAATTATTTTTTCCTCTTTGGCGGTGGAATTAGGCTTTGTCCTGGCAAAGAATTGGGAATTGCAGAGGTTTCTACATTTTTACATTACTTTGTAACTAAATACAGGTACGTAGCTTTCTTTTCCATATTTTATGTATTCTTTTTACTTTTAACTGCTATTTTGATATGCACATTTTTGTCAGTCTCAAATTCCGATTGTATATTTTGTTGGATACGTAAAGATGGGTATGATCCCACATGCACTAAATCCAAAATAAAAACAGAAATTGTGATAAAGAATTTTTGTTTTGTTTTCTGTTGTAGGTGGGAAGAAGTTGGAGAATGTAAGTTCTTGAAATTTCCAAGAGTTAAAGCTCGGAATGGGTTTCACATTAGGGTTTCATCCTATTGA

mRNA sequence

ATGGGCTTAGCCATGGTGATTGTTGGTCTGTTTTTACTCCTCTGTTTTTGCGCTGCTTTACTCCGACGGAATGAAGTTCGATACATAAAAAATGGATTGCCTCCTGGTACAATGGGCTGGCCTGTTTTTGGTGAAACCTCTGAGTTTCTTAAACAAGGTCCGAATTTTATGAATTCTAAAAGAGCAAGGTATGGGAGCTTTTTCAAATCACATATTTTGGGATCTCCTACGGTTGTTTCTATGGATCCAGAGGTTAATAGATATATTTTAATGAATGAATCTAAAGGACTCGTCGCTGGTTATCCTCAATCTATGCTTGATATACTTGGAAAATGCAATATCGCTGCCGTACACGGCGACGCTCATAAGCTTATGAGAGGTGCTCTACTTGCTATTGTTAGTCCCACCATGATTAAAGATCGTCTTCTTCTTCAAGTTGATGAGTTTATGAGATCCCATTTGCGTAATTGGGATAGCCAAATCATTGATATTCAAGAAAAAACTAAACAGATGGCTTTCTGGACATCTTTGAAGCAGACTACCAGTATCGAATCCGGTCCATTATGGGAATCTTTCACCATAGAATTCTTTAAGCTCGTCCTGGGCACTTTCTCGCTCCCAATCGATCTTCCAGGCACGAATTACCGTATAGGAGTTCAGGCAAGGAATAACATAGTAAAGCTATTGAGGCAGCTGATTAATGAGAGAGGAAATTCAAAGATTCAAAAAGATGACATGCTTGGATATTTATTGAGTGAAGAGAACAAATATAAACTAAATGAGGAACAGATTATTGATCAAGTCATTACTGTTTTCTATTCTGGTTATGAAACTGTTTCAACAACTTCAATGATGGCTGTAAAGTTCCTTCATGATCATCCCAAAGTTCTTCAGCAACTCAGAGAAGAACATTTGGCATTAAGAGAGAAGAAAAATCCCGAGGACCCGATCGATTGGGATGATTTTAAAGCAATGGAGTTCACTCGTGCGGTGATTTTTGAAACATCAAGGCTAGCTACTGTTGTTAATGGAGTTTTGAGGAAAACTACAAAGGATTTGGAAGTAAATGGGTTTCTAATTCCTAAGGGATGGAGGATTTATGTGTATAATCGAGAGATAAACTATGACCCTTTTCTCTATTCTGAACCATTCACTTTCAACCCTTGGAGATGGCTGGACAAAAGCTTAGAGTCCCATAATTATTTTTTCCTCTTTGGCGGTGGAATTAGGCTTTGTCCTGGCAAAGAATTGGGAATTGCAGAGGTTTCTACATTTTTACATTACTTTGTAACTAAATACAGGTGGGAAGAAGTTGGAGAATGTAAGTTCTTGAAATTTCCAAGAGTTAAAGCTCGGAATGGGTTTCACATTAGGGTTTCATCCTATTGA

Coding sequence (CDS)

ATGGGCTTAGCCATGGTGATTGTTGGTCTGTTTTTACTCCTCTGTTTTTGCGCTGCTTTACTCCGACGGAATGAAGTTCGATACATAAAAAATGGATTGCCTCCTGGTACAATGGGCTGGCCTGTTTTTGGTGAAACCTCTGAGTTTCTTAAACAAGGTCCGAATTTTATGAATTCTAAAAGAGCAAGGTATGGGAGCTTTTTCAAATCACATATTTTGGGATCTCCTACGGTTGTTTCTATGGATCCAGAGGTTAATAGATATATTTTAATGAATGAATCTAAAGGACTCGTCGCTGGTTATCCTCAATCTATGCTTGATATACTTGGAAAATGCAATATCGCTGCCGTACACGGCGACGCTCATAAGCTTATGAGAGGTGCTCTACTTGCTATTGTTAGTCCCACCATGATTAAAGATCGTCTTCTTCTTCAAGTTGATGAGTTTATGAGATCCCATTTGCGTAATTGGGATAGCCAAATCATTGATATTCAAGAAAAAACTAAACAGATGGCTTTCTGGACATCTTTGAAGCAGACTACCAGTATCGAATCCGGTCCATTATGGGAATCTTTCACCATAGAATTCTTTAAGCTCGTCCTGGGCACTTTCTCGCTCCCAATCGATCTTCCAGGCACGAATTACCGTATAGGAGTTCAGGCAAGGAATAACATAGTAAAGCTATTGAGGCAGCTGATTAATGAGAGAGGAAATTCAAAGATTCAAAAAGATGACATGCTTGGATATTTATTGAGTGAAGAGAACAAATATAAACTAAATGAGGAACAGATTATTGATCAAGTCATTACTGTTTTCTATTCTGGTTATGAAACTGTTTCAACAACTTCAATGATGGCTGTAAAGTTCCTTCATGATCATCCCAAAGTTCTTCAGCAACTCAGAGAAGAACATTTGGCATTAAGAGAGAAGAAAAATCCCGAGGACCCGATCGATTGGGATGATTTTAAAGCAATGGAGTTCACTCGTGCGGTGATTTTTGAAACATCAAGGCTAGCTACTGTTGTTAATGGAGTTTTGAGGAAAACTACAAAGGATTTGGAAGTAAATGGGTTTCTAATTCCTAAGGGATGGAGGATTTATGTGTATAATCGAGAGATAAACTATGACCCTTTTCTCTATTCTGAACCATTCACTTTCAACCCTTGGAGATGGCTGGACAAAAGCTTAGAGTCCCATAATTATTTTTTCCTCTTTGGCGGTGGAATTAGGCTTTGTCCTGGCAAAGAATTGGGAATTGCAGAGGTTTCTACATTTTTACATTACTTTGTAACTAAATACAGGTGGGAAGAAGTTGGAGAATGTAAGTTCTTGAAATTTCCAAGAGTTAAAGCTCGGAATGGGTTTCACATTAGGGTTTCATCCTATTGA

Protein sequence

MGLAMVIVGLFLLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNSKRARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGDAHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQTTSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNSKIQKDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQLREEHLALREKKNPEDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLIPKGWRIYVYNREINYDPFLYSEPFTFNPWRWLDKSLESHNYFFLFGGGIRLCPGKELGIAEVSTFLHYFVTKYRWEEVGECKFLKFPRVKARNGFHIRVSSY*
Homology
BLAST of CsGy4G017590 vs. ExPASy Swiss-Prot
Match: Q69F95 (Cytochrome P450 85A OS=Phaseolus vulgaris OX=3885 GN=BA13 PE=3 SV=2)

HSP 1 Score: 691.0 bits (1782), Expect = 9.0e-198
Identity = 332/464 (71.55%), Postives = 396/464 (85.34%), Query Frame = 0

Query: 1   MGLAMVIVG-LFLLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNS 60
           M L M I+G L LLLCFC+ALL+ NEVR+ + GLPPG MGWPVFGET+EFLKQGPNFM +
Sbjct: 1   MALFMAILGVLVLLLCFCSALLKWNEVRFRRKGLPPGAMGWPVFGETTEFLKQGPNFMKN 60

Query: 61  KRARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHG 120
           KRARYGSFFKSHILG PT+VSMDPE+NR+ILMNE+KGLV GYPQSMLDILG  NIAAVHG
Sbjct: 61  KRARYGSFFKSHILGCPTIVSMDPELNRFILMNEAKGLVPGYPQSMLDILGTRNIAAVHG 120

Query: 121 DAHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQ 180
             HK MRGALL+I+SPT+I+D+LL ++DEFMR+HL +WD+++I+IQEKTK+MAF +SLKQ
Sbjct: 121 STHKYMRGALLSIISPTLIRDQLLPKIDEFMRTHLMDWDNKVINIQEKTKEMAFLSSLKQ 180

Query: 181 TTSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNS 240
              +ES  + + F  EFFKLVLGT SLPI+LP TNYR G+QAR +I+ +L QL+ ER  S
Sbjct: 181 IAGMESSSIAQPFMTEFFKLVLGTLSLPINLPRTNYRGGLQARKSIISILSQLLEERKAS 240

Query: 241 KIQKDDMLGYLL-SEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQ 300
           +    DMLG L+  +EN+YKL++E+IID VIT+ YSGYETVSTTSMMAVK+LHDHPKVL+
Sbjct: 241 QDVHVDMLGCLMKKDENRYKLSDEEIIDLVITIMYSGYETVSTTSMMAVKYLHDHPKVLE 300

Query: 301 QLREEHLALREKKNPEDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGF 360
           ++R+EH A+RE+K PEDPID +D K+M FTRAVIFETSRLAT+VNGVLRKTT D+E+NG+
Sbjct: 301 EIRKEHFAIRERKKPEDPIDCNDLKSMRFTRAVIFETSRLATIVNGVLRKTTHDMELNGY 360

Query: 361 LIPKGWRIYVYNREINYDPFLYSEPFTFNPWRWLDKSLESHNYFFLFGGGIRLCPGKELG 420
           LIPKGWRIYVY REINYDPFLY +P TFNPWRWL  SLES ++F +FGGG R CPGKELG
Sbjct: 361 LIPKGWRIYVYTREINYDPFLYHDPLTFNPWRWLGNSLESQSHFLIFGGGTRQCPGKELG 420

Query: 421 IAEVSTFLHYFVTKYRWEEVGECKFLKFPRVKARNGFHIRVSSY 463
           IAE+STFLHYFVT+YRWEEVG  K +KFPRV A NG HIRVSS+
Sbjct: 421 IAEISTFLHYFVTRYRWEEVGGDKLMKFPRVVAPNGLHIRVSSF 464

BLAST of CsGy4G017590 vs. ExPASy Swiss-Prot
Match: Q43147 (Cytochrome P450 85A1 OS=Solanum lycopersicum OX=4081 GN=CYP85A1 PE=2 SV=1)

HSP 1 Score: 674.1 bits (1738), Expect = 1.1e-192
Identity = 319/464 (68.75%), Postives = 391/464 (84.27%), Query Frame = 0

Query: 1   MGLAMVIVGLFLLLC-FCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNS 60
           M   ++ +  F  LC FC ALLR N+V+Y +  LPPGTMGWP+FGET+EFLK GP+FM +
Sbjct: 1   MAFFLIFLSSFFGLCIFCTALLRWNQVKYNQKNLPPGTMGWPLFGETTEFLKLGPSFMKN 60

Query: 61  KRARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHG 120
           +RARYGSFFKSHILG PT+VSMD E+NRYIL+NE+KGLV GYPQSM+DILGKCNIAAV+G
Sbjct: 61  QRARYGSFFKSHILGCPTIVSMDSELNRYILVNEAKGLVPGYPQSMIDILGKCNIAAVNG 120

Query: 121 DAHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQ 180
            AHK MRGALL+++SPTMI+D+LL ++DEFMRSHL NWD+++IDIQEKT +MAF +SLKQ
Sbjct: 121 SAHKYMRGALLSLISPTMIRDQLLPKIDEFMRSHLTNWDNKVIDIQEKTNKMAFLSSLKQ 180

Query: 181 TTSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNS 240
              IES  L + F  EFF LVLGT SLPI+LP TNY  G QAR  IV LLR LI ER  S
Sbjct: 181 IAGIESTSLAQEFMSEFFNLVLGTLSLPINLPNTNYHRGFQARKIIVNLLRTLIEERRAS 240

Query: 241 KIQKDDMLGYLLSEE-NKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQ 300
           K  + DMLGYL++EE  ++KL ++++ID +IT+ YSGYETVSTTSMMAVK+LHDHPKVL+
Sbjct: 241 KEIQHDMLGYLMNEEATRFKLTDDEMIDLIITILYSGYETVSTTSMMAVKYLHDHPKVLE 300

Query: 301 QLREEHLALREKKNPEDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGF 360
           +LR+EH+A+REKK PEDPID++D+++M FTRAVI ETSRLAT+VNGVLRKTT+D+E+NG+
Sbjct: 301 ELRKEHMAIREKKKPEDPIDYNDYRSMRFTRAVILETSRLATIVNGVLRKTTQDMEINGY 360

Query: 361 LIPKGWRIYVYNREINYDPFLYSEPFTFNPWRWLDKSLESHNYFFLFGGGIRLCPGKELG 420
           +IPKGWRIYVY RE+NYDP LY +P++FNPWRW+DKSLE  N F +FGGG R CPGKELG
Sbjct: 361 IIPKGWRIYVYTRELNYDPRLYPDPYSFNPWRWMDKSLEHQNSFLVFGGGTRQCPGKELG 420

Query: 421 IAEVSTFLHYFVTKYRWEEVGECKFLKFPRVKARNGFHIRVSSY 463
           +AE+STFLHYFVTKYRWEE+G  K +KFPRV+A NG  IRVS++
Sbjct: 421 VAEISTFLHYFVTKYRWEEIGGDKLMKFPRVEAPNGLRIRVSAH 464

BLAST of CsGy4G017590 vs. ExPASy Swiss-Prot
Match: Q940V4 (Cytochrome P450 85A2 OS=Arabidopsis thaliana OX=3702 GN=CYP85A2 PE=2 SV=1)

HSP 1 Score: 667.2 bits (1720), Expect = 1.4e-190
Identity = 317/465 (68.17%), Postives = 387/465 (83.23%), Query Frame = 0

Query: 1   MGLAMVIVGLF-LLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNS 60
           MG+ M+I+GL  +++C C ALLR N++RY K GLPPGTMGWP+FGET+EFLKQGP+FM +
Sbjct: 1   MGIMMMILGLLVIIVCLCTALLRWNQMRYSKKGLPPGTMGWPIFGETTEFLKQGPDFMKN 60

Query: 61  KRARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHG 120
           +R RYGSFFKSHILG PT+VSMD E+NRYILMNESKGLVAGYPQSMLDILG CNIAAVHG
Sbjct: 61  QRLRYGSFFKSHILGCPTIVSMDAELNRYILMNESKGLVAGYPQSMLDILGTCNIAAVHG 120

Query: 121 DAHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDS-QIIDIQEKTKQMAFWTSLK 180
            +H+LMRG+LL+++SPTM+KD LL ++D+FMR++L  WD  + +DIQEKTK MAF +SL 
Sbjct: 121 PSHRLMRGSLLSLISPTMMKDHLLPKIDDFMRNYLCGWDDLETVDIQEKTKHMAFLSSLL 180

Query: 181 QTTSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGN 240
           Q       P  E +  EFFKLV+GT S+PID+PGTNYR GVQARNNI +LL +L+ ER  
Sbjct: 181 QIAETLKKPEVEEYRTEFFKLVVGTLSVPIDIPGTNYRSGVQARNNIDRLLTELMQERKE 240

Query: 241 SKIQKDDMLGYLL-SEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVL 300
           S     DMLGYL+  E+N+Y L +++I DQV+T+ YSGYETVSTTSMMA+K+LHDHPK L
Sbjct: 241 SGETFTDMLGYLMKKEDNRYLLTDKEIRDQVVTILYSGYETVSTTSMMALKYLHDHPKAL 300

Query: 301 QQLREEHLALREKKNPEDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNG 360
           ++LR EHLA+RE+K P++P+  DD K+M+FTRAVIFETSRLAT+VNGVLRKTT DLE+NG
Sbjct: 301 EELRREHLAIRERKRPDEPLTLDDIKSMKFTRAVIFETSRLATIVNGVLRKTTHDLELNG 360

Query: 361 FLIPKGWRIYVYNREINYDPFLYSEPFTFNPWRWLDKSLESHNYFFLFGGGIRLCPGKEL 420
           +LIPKGWRIYVY REINYD  LY +P  FNPWRW++KSLES +YF LFGGG+RLCPGKEL
Sbjct: 361 YLIPKGWRIYVYTREINYDTSLYEDPMIFNPWRWMEKSLESKSYFLLFGGGVRLCPGKEL 420

Query: 421 GIAEVSTFLHYFVTKYRWEEVGECKFLKFPRVKARNGFHIRVSSY 463
           GI+EVS+FLHYFVTKYRWEE GE K + FPRV A  G+H++ S Y
Sbjct: 421 GISEVSSFLHYFVTKYRWEENGEDKLMVFPRVSAPKGYHLKCSPY 465

BLAST of CsGy4G017590 vs. ExPASy Swiss-Prot
Match: Q9FMA5 (Cytochrome P450 85A1 OS=Arabidopsis thaliana OX=3702 GN=CYP85A1 PE=2 SV=1)

HSP 1 Score: 650.2 bits (1676), Expect = 1.8e-185
Identity = 310/465 (66.67%), Postives = 381/465 (81.94%), Query Frame = 0

Query: 1   MGLAMVIVGLFLLL-CFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNS 60
           MG  MV++GL L++   C+ALLR N++RY KNGLPPGTMGWP+FGET+EFLKQGPNFM +
Sbjct: 1   MGAMMVMMGLLLIIVSLCSALLRWNQMRYTKNGLPPGTMGWPIFGETTEFLKQGPNFMRN 60

Query: 61  KRARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHG 120
           +R RYGSFFKSH+LG PT++SMD EVNRYIL NESKGLV GYPQSMLDILG CN+AAVHG
Sbjct: 61  QRLRYGSFFKSHLLGCPTLISMDSEVNRYILKNESKGLVPGYPQSMLDILGTCNMAAVHG 120

Query: 121 DAHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDS-QIIDIQEKTKQMAFWTSLK 180
            +H+LMRG+LL+++S TM++D +L +VD FMRS+L  W+  ++IDIQ+KTK MAF +SL 
Sbjct: 121 SSHRLMRGSLLSLISSTMMRDHILPKVDHFMRSYLDQWNELEVIDIQDKTKHMAFLSSLT 180

Query: 181 QTTSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGN 240
           Q       P  E F   FFKLV+GT S+PIDLPGTNYR G+QARNNI +LLR+L+ ER +
Sbjct: 181 QIAGNLRKPFVEEFKTAFFKLVVGTLSVPIDLPGTNYRCGIQARNNIDRLLRELMQERRD 240

Query: 241 SKIQKDDMLGYLLSEE-NKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVL 300
           S     DMLGYL+ +E N+Y L +E+I DQV+T+ YSGYETVSTTSMMA+K+LHDHPK L
Sbjct: 241 SGETFTDMLGYLMKKEGNRYPLTDEEIRDQVVTILYSGYETVSTTSMMALKYLHDHPKAL 300

Query: 301 QQLREEHLALREKKNPEDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNG 360
           Q+LR EHLA RE+K  ++P+  +D K+M+FTRAVI+ETSRLAT+VNGVLRKTT+DLE+NG
Sbjct: 301 QELRAEHLAFRERKRQDEPLGLEDVKSMKFTRAVIYETSRLATIVNGVLRKTTRDLEING 360

Query: 361 FLIPKGWRIYVYNREINYDPFLYSEPFTFNPWRWLDKSLESHNYFFLFGGGIRLCPGKEL 420
           +LIPKGWRIYVY REINYD  LY +P  FNPWRW+ KSLES N  F+FGGG RLCPGKEL
Sbjct: 361 YLIPKGWRIYVYTREINYDANLYEDPLIFNPWRWMKKSLESQNSCFVFGGGTRLCPGKEL 420

Query: 421 GIAEVSTFLHYFVTKYRWEEVGECKFLKFPRVKARNGFHIRVSSY 463
           GI E+S+FLHYFVT+YRWEE+G  + + FPRV A  GFH+R+S Y
Sbjct: 421 GIVEISSFLHYFVTRYRWEEIGGDELMVFPRVFAPKGFHLRISPY 465

BLAST of CsGy4G017590 vs. ExPASy Swiss-Prot
Match: Q50LE0 (Cytochrome P450 85A3 OS=Solanum lycopersicum OX=4081 GN=CYP85A3 PE=2 SV=1)

HSP 1 Score: 618.2 bits (1593), Expect = 7.4e-176
Identity = 293/467 (62.74%), Postives = 374/467 (80.09%), Query Frame = 0

Query: 1   MGLAMVIVGLFLLLCFCAA-LLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNS 60
           M + ++I  +F   C  +  L R  ++ Y K  LPPGTMGWP+FGET EFL QGPNFM +
Sbjct: 1   MAIFLIIFVVFFGFCILSTPLFRWIDIVYNKKNLPPGTMGWPIFGETREFLNQGPNFMKN 60

Query: 61  KRARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHG 120
           +RARYG+FFKSHILG PTVVSMD  +N YIL NE+KGL+ GYPQSMLDILGKCNIAAVHG
Sbjct: 61  QRARYGNFFKSHILGCPTVVSMDAGLNVYILNNEAKGLIPGYPQSMLDILGKCNIAAVHG 120

Query: 121 DAHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDS-QIIDIQEKTKQMAFWTSLK 180
             HK +RGALL++++PTMIKD +L ++D+FMRSHL  WD+  +IDIQ+ TK+MAF++SL 
Sbjct: 121 ATHKYIRGALLSLINPTMIKDHILPKIDKFMRSHLSGWDNCNVIDIQQMTKEMAFFSSLD 180

Query: 181 QTTSI-ESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERG 240
           Q      S  + + F   F  + LGT SLPI+ P TNY  G+Q R  IVKLLR++I +R 
Sbjct: 181 QIGGFATSSSIAQEFRAGFLNIALGTISLPINFPTTNYYRGLQGRKTIVKLLRKIIEDRR 240

Query: 241 NSKIQKDDMLGYLLSEE--NKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPK 300
            SK  + DMLG +++EE  N+Y L++E++IDQ+IT+ YSG+ETVSTTSMMAVK+LHDHPK
Sbjct: 241 GSKKIQQDMLGLMMNEEAKNRYTLSDEELIDQIITIMYSGFETVSTTSMMAVKYLHDHPK 300

Query: 301 VLQQLREEHLALREKKNPEDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEV 360
            L+++R+EH A+REKK+ EDPID++DFKAM FTRAVI+ET RLAT+VNGVLRKTT+D+E+
Sbjct: 301 ALEEIRKEHFAIREKKSLEDPIDYNDFKAMRFTRAVIYETLRLATIVNGVLRKTTQDMEL 360

Query: 361 NGFLIPKGWRIYVYNREINYDPFLYSEPFTFNPWRWLDKSLESHNYFFLFGGGIRLCPGK 420
           NG++IPKGWRIYVY RE+NYDP +Y +P+TFNPWRWL+ +L+  + F +FGGG RLCPGK
Sbjct: 361 NGYMIPKGWRIYVYTRELNYDPLIYPDPYTFNPWRWLENNLDHQSSFLMFGGGTRLCPGK 420

Query: 421 ELGIAEVSTFLHYFVTKYRWEEVGECKFLKFPRVKARNGFHIRVSSY 463
           ELG+AE+STFLHYFVT+YRWEEVG  K +KFPRV+A NG  I+VS+Y
Sbjct: 421 ELGVAEISTFLHYFVTRYRWEEVGGNKLMKFPRVEALNGLWIKVSAY 467

BLAST of CsGy4G017590 vs. NCBI nr
Match: XP_004150514.3 (cytochrome P450 85A [Cucumis sativus])

HSP 1 Score: 939 bits (2426), Expect = 0.0
Identity = 459/462 (99.35%), Postives = 461/462 (99.78%), Query Frame = 0

Query: 1   MGLAMVIVGLFLLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNSK 60
           MGLAMVIVGLFLLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNSK
Sbjct: 1   MGLAMVIVGLFLLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNSK 60

Query: 61  RARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGD 120
           RARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGD
Sbjct: 61  RARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGD 120

Query: 121 AHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180
           AHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT
Sbjct: 121 AHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180

Query: 181 TSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNSK 240
           TSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNSK
Sbjct: 181 TSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNSK 240

Query: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           IQKDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHLALREKKNPEDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI 360
           REEHLALREKKNP+DPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI
Sbjct: 301 REEHLALREKKNPKDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI 360

Query: 361 PKGWRIYVYNREINYDPFLYSEPFTFNPWRWLDKSLESHNYFFLFGGGIRLCPGKELGIA 420
           PKGWRIYVYNREINYDPFLYSEP+TFNPWRWLDKSLESHNYFFLFGGGIRLCPGKELGIA
Sbjct: 361 PKGWRIYVYNREINYDPFLYSEPYTFNPWRWLDKSLESHNYFFLFGGGIRLCPGKELGIA 420

Query: 421 EVSTFLHYFVTKYRWEEVGECKFLKFPRVKARNGFHIRVSSY 462
           EVSTFLHYFVTKYRWEEVGECK LKFPRVKARNGFHIRVSSY
Sbjct: 421 EVSTFLHYFVTKYRWEEVGECKLLKFPRVKARNGFHIRVSSY 462

BLAST of CsGy4G017590 vs. NCBI nr
Match: XP_008455791.1 (PREDICTED: cytochrome P450 85A-like [Cucumis melo])

HSP 1 Score: 897 bits (2318), Expect = 0.0
Identity = 437/462 (94.59%), Postives = 448/462 (96.97%), Query Frame = 0

Query: 1   MGLAMVIVGLFLLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNSK 60
           M LA+VI+ LFLLLCFCAAL + NEVRY KNGLPPGTMGWPVFGETSEFLKQGPNFMNSK
Sbjct: 1   MALAIVILALFLLLCFCAALFQWNEVRYRKNGLPPGTMGWPVFGETSEFLKQGPNFMNSK 60

Query: 61  RARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGD 120
           RARYGSFFKSHILGSPTV+SMDPEVNRYILMNESKGLVAGYPQSMLDILG CNIAAVHGD
Sbjct: 61  RARYGSFFKSHILGSPTVISMDPEVNRYILMNESKGLVAGYPQSMLDILGTCNIAAVHGD 120

Query: 121 AHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180
           AHKLMRGALLAIV PTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT
Sbjct: 121 AHKLMRGALLAIVGPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180

Query: 181 TSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNSK 240
           TSI SGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLL++LI+ER NSK
Sbjct: 181 TSIVSGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLKKLISERVNSK 240

Query: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           IQKDDMLGYLLSEENKYKLNEEQIIDQ+ITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQIITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHLALREKKNPEDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI 360
           REEH+ALREKKNPEDPIDWDDFKAM+FTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI
Sbjct: 301 REEHIALREKKNPEDPIDWDDFKAMDFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI 360

Query: 361 PKGWRIYVYNREINYDPFLYSEPFTFNPWRWLDKSLESHNYFFLFGGGIRLCPGKELGIA 420
           PKGWRIYVY REINYDPFLYSEPFTFNPWRWLDK+LESHNYFFLFGGG RLCPGKELGIA
Sbjct: 361 PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFLFGGGTRLCPGKELGIA 420

Query: 421 EVSTFLHYFVTKYRWEEVGECKFLKFPRVKARNGFHIRVSSY 462
           EVSTFLHYFVTKYRWEEVG CK LKFPRVKA NGFHIRVSSY
Sbjct: 421 EVSTFLHYFVTKYRWEEVGGCKLLKFPRVKAPNGFHIRVSSY 462

BLAST of CsGy4G017590 vs. NCBI nr
Match: KAE8649648.1 (hypothetical protein Csa_012605 [Cucumis sativus])

HSP 1 Score: 869 bits (2245), Expect = 0.0
Identity = 435/462 (94.16%), Postives = 436/462 (94.37%), Query Frame = 0

Query: 1   MGLAMVIVGLFLLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNSK 60
           MGLAMVIVGLFLLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNSK
Sbjct: 1   MGLAMVIVGLFLLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNSK 60

Query: 61  RARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGD 120
           RARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGD
Sbjct: 61  RARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGD 120

Query: 121 AHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180
           AHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT
Sbjct: 121 AHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180

Query: 181 TSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNSK 240
           TSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNSK
Sbjct: 181 TSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNSK 240

Query: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           IQKDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHLALREKKNPEDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI 360
           REEHLALREKKNP+DPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI
Sbjct: 301 REEHLALREKKNPKDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI 360

Query: 361 PKGWRIYVYNREINYDPFLYSEPFTFNPWRWLDKSLESHNYFFLFGGGIRLCPGKELGIA 420
           PKGWRIY                         DKSLESHNYFFLFGGGIRLCPGKELGIA
Sbjct: 361 PKGWRIY-------------------------DKSLESHNYFFLFGGGIRLCPGKELGIA 420

Query: 421 EVSTFLHYFVTKYRWEEVGECKFLKFPRVKARNGFHIRVSSY 462
           EVSTFLHYFVTKYRWEEVGECK LKFPRVKARNGFHIRVSSY
Sbjct: 421 EVSTFLHYFVTKYRWEEVGECKLLKFPRVKARNGFHIRVSSY 437

BLAST of CsGy4G017590 vs. NCBI nr
Match: XP_038890036.1 (cytochrome P450 85A-like [Benincasa hispida])

HSP 1 Score: 828 bits (2140), Expect = 2.82e-301
Identity = 401/462 (86.80%), Postives = 430/462 (93.07%), Query Frame = 0

Query: 1   MGLAMVIVGLFLLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNSK 60
           M LAMVI+ LFLLLCFC+ALLR NEVRY K GLPPGTMGWPVFGET+EFLKQGPNFM S+
Sbjct: 1   MALAMVILALFLLLCFCSALLRWNEVRYSKKGLPPGTMGWPVFGETTEFLKQGPNFMKSQ 60

Query: 61  RARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGD 120
           RARYGS FKSHILG PT+VSMDPEVNRY+LMNESKGLV GYPQSMLDILGKCNIAAVHG 
Sbjct: 61  RARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120

Query: 121 AHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180
           AHKLMRGALLAIVSPTMIKDRLL +VD+FMRSHLRNWDSQIIDIQEKTK+MA  +SLKQ 
Sbjct: 121 AHKLMRGALLAIVSPTMIKDRLLPKVDDFMRSHLRNWDSQIIDIQEKTKEMALRSSLKQI 180

Query: 181 TSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNSK 240
             IESGPL ESF  EFFKLVLGT SLPIDLPGTNYR G+QAR NI+KLL+QLINER +SK
Sbjct: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRDSK 240

Query: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           IQKDDMLG+L+SEENKYKLN+E+IIDQ+ITV YSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 IQKDDMLGFLMSEENKYKLNDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHLALREKKNPEDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI 360
           REEHLA+REKKNPEDPIDWDD KAM+FTRAVIFETSRLAT+VNGVLRKTTKD+E+NGFLI
Sbjct: 301 REEHLAIREKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360

Query: 361 PKGWRIYVYNREINYDPFLYSEPFTFNPWRWLDKSLESHNYFFLFGGGIRLCPGKELGIA 420
           PKGWRIYVY REINYDPFLYSEPFTFNPWRWLDK+LESHNYFF+FGGG R CPGKELGIA
Sbjct: 361 PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFIFGGGTRHCPGKELGIA 420

Query: 421 EVSTFLHYFVTKYRWEEVGECKFLKFPRVKARNGFHIRVSSY 462
           E+STFLHYFVTKYRWEEVG  K LKFPRV+A +GFHIRVSSY
Sbjct: 421 EISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 462

BLAST of CsGy4G017590 vs. NCBI nr
Match: KAA0040382.1 (cytochrome P450 85A-like [Cucumis melo var. makuwa] >TYK04049.1 cytochrome P450 85A-like [Cucumis melo var. makuwa])

HSP 1 Score: 820 bits (2119), Expect = 1.33e-298
Identity = 401/428 (93.69%), Postives = 414/428 (96.73%), Query Frame = 0

Query: 1   MGLAMVIVGLFLLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNSK 60
           M LA+VI+ LFLLLCFCAAL + NEVRY KNGLPPGTMGWPVFGETSEFLKQGPNFMNSK
Sbjct: 1   MALAIVILALFLLLCFCAALFQWNEVRYRKNGLPPGTMGWPVFGETSEFLKQGPNFMNSK 60

Query: 61  RARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGD 120
           RARYGSFFKSHILGSPTV+SMDPEVNRYILMNESKGLVAGYPQSMLDILG CNIAAVHGD
Sbjct: 61  RARYGSFFKSHILGSPTVISMDPEVNRYILMNESKGLVAGYPQSMLDILGTCNIAAVHGD 120

Query: 121 AHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180
           AHKLMRGALLAIV PTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT
Sbjct: 121 AHKLMRGALLAIVGPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180

Query: 181 TSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNSK 240
           TSI SGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLL++LI+ER NSK
Sbjct: 181 TSIVSGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLKKLISERVNSK 240

Query: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           IQKDDMLGYLLSEENKYKLNEEQIIDQ+ITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQIITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHLALREKKNPEDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI 360
           REEH+ALREKKNPEDPIDWDDFKAM+FTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI
Sbjct: 301 REEHIALREKKNPEDPIDWDDFKAMDFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI 360

Query: 361 PKGWRIYVYNREINYDPFLYSEPFTFNPWRWLDKSLESHNYFFLFGGGIRLCPGKELGIA 420
           PKGWRIYVY REINYDPFLYSEPFTFNPWRWLDK+LESHNYFFLFGGG RLCPGKELGIA
Sbjct: 361 PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFLFGGGTRLCPGKELGIA 420

Query: 421 EVSTFLHY 428
           E+  F+ Y
Sbjct: 421 EIVYFVGY 428

BLAST of CsGy4G017590 vs. ExPASy TrEMBL
Match: A0A0A0L0Q5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G410870 PE=3 SV=1)

HSP 1 Score: 929 bits (2400), Expect = 0.0
Identity = 454/458 (99.13%), Postives = 456/458 (99.56%), Query Frame = 0

Query: 5   MVIVGLFLLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNSKRARY 64
           MVIVGLFLLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNSKRARY
Sbjct: 1   MVIVGLFLLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNSKRARY 60

Query: 65  GSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGDAHKL 124
           GSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGDAHKL
Sbjct: 61  GSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGDAHKL 120

Query: 125 MRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQTTSIE 184
           MRGALLAIVS TMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQTTSIE
Sbjct: 121 MRGALLAIVSHTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQTTSIE 180

Query: 185 SGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNSKIQKD 244
           SGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNSKIQKD
Sbjct: 181 SGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNSKIQKD 240

Query: 245 DMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQLREEH 304
           DMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQLREEH
Sbjct: 241 DMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQLREEH 300

Query: 305 LALREKKNPEDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLIPKGW 364
           LALREKKNP+DPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLIPKGW
Sbjct: 301 LALREKKNPKDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLIPKGW 360

Query: 365 RIYVYNREINYDPFLYSEPFTFNPWRWLDKSLESHNYFFLFGGGIRLCPGKELGIAEVST 424
           RIYVYNREINYDPFLYSEP+TFNPWRWLDKSLESHNYFFLFGGGIRLCPGKELGIAEVST
Sbjct: 361 RIYVYNREINYDPFLYSEPYTFNPWRWLDKSLESHNYFFLFGGGIRLCPGKELGIAEVST 420

Query: 425 FLHYFVTKYRWEEVGECKFLKFPRVKARNGFHIRVSSY 462
           FLHYFVTKYRWEEVGECK LKFPRVKARNGFHIRVSSY
Sbjct: 421 FLHYFVTKYRWEEVGECKLLKFPRVKARNGFHIRVSSY 458

BLAST of CsGy4G017590 vs. ExPASy TrEMBL
Match: A0A1S3C1A8 (cytochrome P450 85A-like OS=Cucumis melo OX=3656 GN=LOC103495886 PE=3 SV=1)

HSP 1 Score: 897 bits (2318), Expect = 0.0
Identity = 437/462 (94.59%), Postives = 448/462 (96.97%), Query Frame = 0

Query: 1   MGLAMVIVGLFLLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNSK 60
           M LA+VI+ LFLLLCFCAAL + NEVRY KNGLPPGTMGWPVFGETSEFLKQGPNFMNSK
Sbjct: 1   MALAIVILALFLLLCFCAALFQWNEVRYRKNGLPPGTMGWPVFGETSEFLKQGPNFMNSK 60

Query: 61  RARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGD 120
           RARYGSFFKSHILGSPTV+SMDPEVNRYILMNESKGLVAGYPQSMLDILG CNIAAVHGD
Sbjct: 61  RARYGSFFKSHILGSPTVISMDPEVNRYILMNESKGLVAGYPQSMLDILGTCNIAAVHGD 120

Query: 121 AHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180
           AHKLMRGALLAIV PTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT
Sbjct: 121 AHKLMRGALLAIVGPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180

Query: 181 TSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNSK 240
           TSI SGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLL++LI+ER NSK
Sbjct: 181 TSIVSGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLKKLISERVNSK 240

Query: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           IQKDDMLGYLLSEENKYKLNEEQIIDQ+ITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQIITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHLALREKKNPEDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI 360
           REEH+ALREKKNPEDPIDWDDFKAM+FTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI
Sbjct: 301 REEHIALREKKNPEDPIDWDDFKAMDFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI 360

Query: 361 PKGWRIYVYNREINYDPFLYSEPFTFNPWRWLDKSLESHNYFFLFGGGIRLCPGKELGIA 420
           PKGWRIYVY REINYDPFLYSEPFTFNPWRWLDK+LESHNYFFLFGGG RLCPGKELGIA
Sbjct: 361 PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFLFGGGTRLCPGKELGIA 420

Query: 421 EVSTFLHYFVTKYRWEEVGECKFLKFPRVKARNGFHIRVSSY 462
           EVSTFLHYFVTKYRWEEVG CK LKFPRVKA NGFHIRVSSY
Sbjct: 421 EVSTFLHYFVTKYRWEEVGGCKLLKFPRVKAPNGFHIRVSSY 462

BLAST of CsGy4G017590 vs. ExPASy TrEMBL
Match: A0A5A7TA97 (Cytochrome P450 85A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold460G00020 PE=3 SV=1)

HSP 1 Score: 820 bits (2119), Expect = 6.44e-299
Identity = 401/428 (93.69%), Postives = 414/428 (96.73%), Query Frame = 0

Query: 1   MGLAMVIVGLFLLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNSK 60
           M LA+VI+ LFLLLCFCAAL + NEVRY KNGLPPGTMGWPVFGETSEFLKQGPNFMNSK
Sbjct: 1   MALAIVILALFLLLCFCAALFQWNEVRYRKNGLPPGTMGWPVFGETSEFLKQGPNFMNSK 60

Query: 61  RARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGD 120
           RARYGSFFKSHILGSPTV+SMDPEVNRYILMNESKGLVAGYPQSMLDILG CNIAAVHGD
Sbjct: 61  RARYGSFFKSHILGSPTVISMDPEVNRYILMNESKGLVAGYPQSMLDILGTCNIAAVHGD 120

Query: 121 AHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180
           AHKLMRGALLAIV PTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT
Sbjct: 121 AHKLMRGALLAIVGPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180

Query: 181 TSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNSK 240
           TSI SGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLL++LI+ER NSK
Sbjct: 181 TSIVSGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLKKLISERVNSK 240

Query: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           IQKDDMLGYLLSEENKYKLNEEQIIDQ+ITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQIITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHLALREKKNPEDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI 360
           REEH+ALREKKNPEDPIDWDDFKAM+FTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI
Sbjct: 301 REEHIALREKKNPEDPIDWDDFKAMDFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLI 360

Query: 361 PKGWRIYVYNREINYDPFLYSEPFTFNPWRWLDKSLESHNYFFLFGGGIRLCPGKELGIA 420
           PKGWRIYVY REINYDPFLYSEPFTFNPWRWLDK+LESHNYFFLFGGG RLCPGKELGIA
Sbjct: 361 PKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDKNLESHNYFFLFGGGTRLCPGKELGIA 420

Query: 421 EVSTFLHY 428
           E+  F+ Y
Sbjct: 421 EIVYFVGY 428

BLAST of CsGy4G017590 vs. ExPASy TrEMBL
Match: A0A0A0KXL9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G641590 PE=3 SV=1)

HSP 1 Score: 811 bits (2095), Expect = 1.01e-294
Identity = 393/463 (84.88%), Postives = 424/463 (91.58%), Query Frame = 0

Query: 1   MGLAMVIVGLFLLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNSK 60
           M LAMVI+ LFLLL FC+ALLR NEVRY K GLPPGTMGWP+FGET+EFLKQGPNFM S+
Sbjct: 1   MALAMVIIALFLLLLFCSALLRWNEVRYSKKGLPPGTMGWPIFGETTEFLKQGPNFMKSQ 60

Query: 61  RARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGD 120
           RARYGS FKSHILG PT+VSMDPEVNRY+LMNESKGLV GYPQSMLDILGKCNIAAVHG 
Sbjct: 61  RARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGS 120

Query: 121 AHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180
           AHKLMRGALLAIVSPTMIKDRLL  +DEFMRSHL NWD+QIIDIQEKTK+MA  +S+KQ 
Sbjct: 121 AHKLMRGALLAIVSPTMIKDRLLPNIDEFMRSHLSNWDNQIIDIQEKTKEMALRSSMKQI 180

Query: 181 TSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNSK 240
              ESGPL ESFT EFFKLVLGT SLPIDLPGTNYR G+QAR NI+KLL QLI ER +SK
Sbjct: 181 AGFESGPLSESFTPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLEQLIKERRDSK 240

Query: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           IQK+DMLGY +SEENKYKLN+E+IIDQ+ITV YSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 IQKNDMLGYFMSEENKYKLNDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHLALREKK-NPEDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFL 360
           REEHLA+REKK NPEDPIDWDD KAM+FTRAVIFETSRLAT+VNGVLRKTTKD+E+NGFL
Sbjct: 301 REEHLAIREKKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFL 360

Query: 361 IPKGWRIYVYNREINYDPFLYSEPFTFNPWRWLDKSLESHNYFFLFGGGIRLCPGKELGI 420
           IPKGWRIYVY REINYDPFLYSEPFTFNPWRWLD +LES+NYFF+FGGG RLCPGKELGI
Sbjct: 361 IPKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDNNLESNNYFFIFGGGTRLCPGKELGI 420

Query: 421 AEVSTFLHYFVTKYRWEEVGECKFLKFPRVKARNGFHIRVSSY 462
           AE+STFLHYFVTKYRWEEVG  K LKFPRV+A +GFHIRVSSY
Sbjct: 421 AEISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 463

BLAST of CsGy4G017590 vs. ExPASy TrEMBL
Match: A0A5A7TDB4 (Cytochrome P450 85A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold64G002300 PE=3 SV=1)

HSP 1 Score: 808 bits (2088), Expect = 1.18e-293
Identity = 392/463 (84.67%), Postives = 424/463 (91.58%), Query Frame = 0

Query: 1   MGLAMVIVGLFLLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNSK 60
           M LAMVI+ LFLLL FC+ALLR NEVRY K GLPPGTMGWP+FGET+EFLKQGPNFM S+
Sbjct: 1   MALAMVILALFLLLLFCSALLRWNEVRYSKKGLPPGTMGWPIFGETTEFLKQGPNFMKSQ 60

Query: 61  RARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGD 120
           RARYGS FKSHILG PT+VSMDPEVNRY+LMNESKGLV GYPQSMLDILGKCNIAAVHG 
Sbjct: 61  RARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGS 120

Query: 121 AHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSLKQT 180
           AHKLMRGALLAIVSPTMIKDRLL ++DEFMRSHL NWD+QIIDIQEKTK+MA  +S+KQ 
Sbjct: 121 AHKLMRGALLAIVSPTMIKDRLLPKLDEFMRSHLSNWDNQIIDIQEKTKEMALRSSMKQI 180

Query: 181 TSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNSK 240
              ESGPL ESFT EFFKLVLGT SLPIDLPGTNYR G+QAR NI+KLL QLI ER +SK
Sbjct: 181 AGFESGPLSESFTPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLEQLIKERRDSK 240

Query: 241 IQKDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           IQK+DMLGY +SEENKYKLN+E+IIDQ+ITV YSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 IQKNDMLGYFMSEENKYKLNDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHLALREKK-NPEDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFL 360
           REEHLA+REKK NPEDPIDWDD KAM+FTRAVIFETSRLAT+VNGVLRKTTKD+E+NGFL
Sbjct: 301 REEHLAIREKKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFL 360

Query: 361 IPKGWRIYVYNREINYDPFLYSEPFTFNPWRWLDKSLESHNYFFLFGGGIRLCPGKELGI 420
           IPKGWRIYVY REINYDPFLYSEPFTFNPWRWLD +LES+NYFF+FGGG RLCPGKELGI
Sbjct: 361 IPKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDNNLESNNYFFIFGGGTRLCPGKELGI 420

Query: 421 AEVSTFLHYFVTKYRWEEVGECKFLKFPRVKARNGFHIRVSSY 462
            E+STFLHYFVTKYRWEEVG  K LKFPRV+A +GFHIRVSSY
Sbjct: 421 TEISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 463

BLAST of CsGy4G017590 vs. TAIR 10
Match: AT3G30180.1 (brassinosteroid-6-oxidase 2 )

HSP 1 Score: 667.2 bits (1720), Expect = 9.9e-192
Identity = 317/465 (68.17%), Postives = 387/465 (83.23%), Query Frame = 0

Query: 1   MGLAMVIVGLF-LLLCFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNS 60
           MG+ M+I+GL  +++C C ALLR N++RY K GLPPGTMGWP+FGET+EFLKQGP+FM +
Sbjct: 1   MGIMMMILGLLVIIVCLCTALLRWNQMRYSKKGLPPGTMGWPIFGETTEFLKQGPDFMKN 60

Query: 61  KRARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHG 120
           +R RYGSFFKSHILG PT+VSMD E+NRYILMNESKGLVAGYPQSMLDILG CNIAAVHG
Sbjct: 61  QRLRYGSFFKSHILGCPTIVSMDAELNRYILMNESKGLVAGYPQSMLDILGTCNIAAVHG 120

Query: 121 DAHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDS-QIIDIQEKTKQMAFWTSLK 180
            +H+LMRG+LL+++SPTM+KD LL ++D+FMR++L  WD  + +DIQEKTK MAF +SL 
Sbjct: 121 PSHRLMRGSLLSLISPTMMKDHLLPKIDDFMRNYLCGWDDLETVDIQEKTKHMAFLSSLL 180

Query: 181 QTTSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGN 240
           Q       P  E +  EFFKLV+GT S+PID+PGTNYR GVQARNNI +LL +L+ ER  
Sbjct: 181 QIAETLKKPEVEEYRTEFFKLVVGTLSVPIDIPGTNYRSGVQARNNIDRLLTELMQERKE 240

Query: 241 SKIQKDDMLGYLL-SEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVL 300
           S     DMLGYL+  E+N+Y L +++I DQV+T+ YSGYETVSTTSMMA+K+LHDHPK L
Sbjct: 241 SGETFTDMLGYLMKKEDNRYLLTDKEIRDQVVTILYSGYETVSTTSMMALKYLHDHPKAL 300

Query: 301 QQLREEHLALREKKNPEDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNG 360
           ++LR EHLA+RE+K P++P+  DD K+M+FTRAVIFETSRLAT+VNGVLRKTT DLE+NG
Sbjct: 301 EELRREHLAIRERKRPDEPLTLDDIKSMKFTRAVIFETSRLATIVNGVLRKTTHDLELNG 360

Query: 361 FLIPKGWRIYVYNREINYDPFLYSEPFTFNPWRWLDKSLESHNYFFLFGGGIRLCPGKEL 420
           +LIPKGWRIYVY REINYD  LY +P  FNPWRW++KSLES +YF LFGGG+RLCPGKEL
Sbjct: 361 YLIPKGWRIYVYTREINYDTSLYEDPMIFNPWRWMEKSLESKSYFLLFGGGVRLCPGKEL 420

Query: 421 GIAEVSTFLHYFVTKYRWEEVGECKFLKFPRVKARNGFHIRVSSY 463
           GI+EVS+FLHYFVTKYRWEE GE K + FPRV A  G+H++ S Y
Sbjct: 421 GISEVSSFLHYFVTKYRWEENGEDKLMVFPRVSAPKGYHLKCSPY 465

BLAST of CsGy4G017590 vs. TAIR 10
Match: AT5G38970.1 (brassinosteroid-6-oxidase 1 )

HSP 1 Score: 650.2 bits (1676), Expect = 1.2e-186
Identity = 310/465 (66.67%), Postives = 381/465 (81.94%), Query Frame = 0

Query: 1   MGLAMVIVGLFLLL-CFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNS 60
           MG  MV++GL L++   C+ALLR N++RY KNGLPPGTMGWP+FGET+EFLKQGPNFM +
Sbjct: 1   MGAMMVMMGLLLIIVSLCSALLRWNQMRYTKNGLPPGTMGWPIFGETTEFLKQGPNFMRN 60

Query: 61  KRARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHG 120
           +R RYGSFFKSH+LG PT++SMD EVNRYIL NESKGLV GYPQSMLDILG CN+AAVHG
Sbjct: 61  QRLRYGSFFKSHLLGCPTLISMDSEVNRYILKNESKGLVPGYPQSMLDILGTCNMAAVHG 120

Query: 121 DAHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDS-QIIDIQEKTKQMAFWTSLK 180
            +H+LMRG+LL+++S TM++D +L +VD FMRS+L  W+  ++IDIQ+KTK MAF +SL 
Sbjct: 121 SSHRLMRGSLLSLISSTMMRDHILPKVDHFMRSYLDQWNELEVIDIQDKTKHMAFLSSLT 180

Query: 181 QTTSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGN 240
           Q       P  E F   FFKLV+GT S+PIDLPGTNYR G+QARNNI +LLR+L+ ER +
Sbjct: 181 QIAGNLRKPFVEEFKTAFFKLVVGTLSVPIDLPGTNYRCGIQARNNIDRLLRELMQERRD 240

Query: 241 SKIQKDDMLGYLLSEE-NKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVL 300
           S     DMLGYL+ +E N+Y L +E+I DQV+T+ YSGYETVSTTSMMA+K+LHDHPK L
Sbjct: 241 SGETFTDMLGYLMKKEGNRYPLTDEEIRDQVVTILYSGYETVSTTSMMALKYLHDHPKAL 300

Query: 301 QQLREEHLALREKKNPEDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNG 360
           Q+LR EHLA RE+K  ++P+  +D K+M+FTRAVI+ETSRLAT+VNGVLRKTT+DLE+NG
Sbjct: 301 QELRAEHLAFRERKRQDEPLGLEDVKSMKFTRAVIYETSRLATIVNGVLRKTTRDLEING 360

Query: 361 FLIPKGWRIYVYNREINYDPFLYSEPFTFNPWRWLDKSLESHNYFFLFGGGIRLCPGKEL 420
           +LIPKGWRIYVY REINYD  LY +P  FNPWRW+ KSLES N  F+FGGG RLCPGKEL
Sbjct: 361 YLIPKGWRIYVYTREINYDANLYEDPLIFNPWRWMKKSLESQNSCFVFGGGTRLCPGKEL 420

Query: 421 GIAEVSTFLHYFVTKYRWEEVGECKFLKFPRVKARNGFHIRVSSY 463
           GI E+S+FLHYFVT+YRWEE+G  + + FPRV A  GFH+R+S Y
Sbjct: 421 GIVEISSFLHYFVTRYRWEEIGGDELMVFPRVFAPKGFHLRISPY 465

BLAST of CsGy4G017590 vs. TAIR 10
Match: AT5G38970.3 (brassinosteroid-6-oxidase 1 )

HSP 1 Score: 544.7 bits (1402), Expect = 7.4e-155
Identity = 263/395 (66.58%), Postives = 325/395 (82.28%), Query Frame = 0

Query: 1   MGLAMVIVGLFLLL-CFCAALLRRNEVRYIKNGLPPGTMGWPVFGETSEFLKQGPNFMNS 60
           MG  MV++GL L++   C+ALLR N++RY KNGLPPGTMGWP+FGET+EFLKQGPNFM +
Sbjct: 1   MGAMMVMMGLLLIIVSLCSALLRWNQMRYTKNGLPPGTMGWPIFGETTEFLKQGPNFMRN 60

Query: 61  KRARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHG 120
           +R RYGSFFKSH+LG PT++SMD EVNRYIL NESKGLV GYPQSMLDILG CN+AAVHG
Sbjct: 61  QRLRYGSFFKSHLLGCPTLISMDSEVNRYILKNESKGLVPGYPQSMLDILGTCNMAAVHG 120

Query: 121 DAHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDS-QIIDIQEKTKQMAFWTSLK 180
            +H+LMRG+LL+++S TM++D +L +VD FMRS+L  W+  ++IDIQ+KTK MAF +SL 
Sbjct: 121 SSHRLMRGSLLSLISSTMMRDHILPKVDHFMRSYLDQWNELEVIDIQDKTKHMAFLSSLT 180

Query: 181 QTTSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGN 240
           Q       P  E F   FFKLV+GT S+PIDLPGTNYR G+QARNNI +LLR+L+ ER +
Sbjct: 181 QIAGNLRKPFVEEFKTAFFKLVVGTLSVPIDLPGTNYRCGIQARNNIDRLLRELMQERRD 240

Query: 241 SKIQKDDMLGYLLSEE-NKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVL 300
           S     DMLGYL+ +E N+Y L +E+I DQV+T+ YSGYETVSTTSMMA+K+LHDHPK L
Sbjct: 241 SGETFTDMLGYLMKKEGNRYPLTDEEIRDQVVTILYSGYETVSTTSMMALKYLHDHPKAL 300

Query: 301 QQLREEHLALREKKNPEDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNG 360
           Q+LR EHLA RE+K  ++P+  +D K+M+FTRAVI+ETSRLAT+VNGVLRKTT+DLE+NG
Sbjct: 301 QELRAEHLAFRERKRQDEPLGLEDVKSMKFTRAVIYETSRLATIVNGVLRKTTRDLEING 360

Query: 361 FLIPKGWRIYVYNREINYDPFLYSEPFTFNPWRWL 393
           +LIPKGWRIYVY REINYD  LY +P  FNPWRW+
Sbjct: 361 YLIPKGWRIYVYTREINYDANLYEDPLIFNPWRWM 395

BLAST of CsGy4G017590 vs. TAIR 10
Match: AT5G38970.2 (brassinosteroid-6-oxidase 1 )

HSP 1 Score: 530.4 bits (1365), Expect = 1.4e-150
Identity = 254/384 (66.15%), Postives = 311/384 (80.99%), Query Frame = 0

Query: 81  MDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAVHGDAHKLMRGALLAIVSPTMIKD 140
           MD EVNRYIL NESKGLV GYPQSMLDILG CN+AAVHG +H+LMRG+LL+++S TM++D
Sbjct: 1   MDSEVNRYILKNESKGLVPGYPQSMLDILGTCNMAAVHGSSHRLMRGSLLSLISSTMMRD 60

Query: 141 RLLLQVDEFMRSHLRNWDS-QIIDIQEKTKQMAFWTSLKQTTSIESGPLWESFTIEFFKL 200
            +L +VD FMRS+L  W+  ++IDIQ+KTK MAF +SL Q       P  E F   FFKL
Sbjct: 61  HILPKVDHFMRSYLDQWNELEVIDIQDKTKHMAFLSSLTQIAGNLRKPFVEEFKTAFFKL 120

Query: 201 VLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERGNSKIQKDDMLGYLLSEE-NKYK 260
           V+GT S+PIDLPGTNYR G+QARNNI +LLR+L+ ER +S     DMLGYL+ +E N+Y 
Sbjct: 121 VVGTLSVPIDLPGTNYRCGIQARNNIDRLLRELMQERRDSGETFTDMLGYLMKKEGNRYP 180

Query: 261 LNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDHPKVLQQLREEHLALREKKNPEDPID 320
           L +E+I DQV+T+ YSGYETVSTTSMMA+K+LHDHPK LQ+LR EHLA RE+K  ++P+ 
Sbjct: 181 LTDEEIRDQVVTILYSGYETVSTTSMMALKYLHDHPKALQELRAEHLAFRERKRQDEPLG 240

Query: 321 WDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDLEVNGFLIPKGWRIYVYNREINYDPF 380
            +D K+M+FTRAVI+ETSRLAT+VNGVLRKTT+DLE+NG+LIPKGWRIYVY REINYD  
Sbjct: 241 LEDVKSMKFTRAVIYETSRLATIVNGVLRKTTRDLEINGYLIPKGWRIYVYTREINYDAN 300

Query: 381 LYSEPFTFNPWRWLDKSLESHNYFFLFGGGIRLCPGKELGIAEVSTFLHYFVTKYRWEEV 440
           LY +P  FNPWRW+ KSLES N  F+FGGG RLCPGKELGI E+S+FLHYFVT+YRWEE+
Sbjct: 301 LYEDPLIFNPWRWMKKSLESQNSCFVFGGGTRLCPGKELGIVEISSFLHYFVTRYRWEEI 360

Query: 441 GECKFLKFPRVKARNGFHIRVSSY 463
           G  + + FPRV A  GFH+R+S Y
Sbjct: 361 GGDELMVFPRVFAPKGFHLRISPY 384

BLAST of CsGy4G017590 vs. TAIR 10
Match: AT5G05690.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 328.9 bits (842), Expect = 6.4e-90
Identity = 173/468 (36.97%), Postives = 272/468 (58.12%), Query Frame = 0

Query: 5   MVIVGLFLLLCFCAA--LLRRNEVRYIKNGLPPGTMGWPVFGETSEFL----KQGPN-FM 64
           M      LLL   AA  LL     RY + GLPPG++G P+ GET + +     + P  F+
Sbjct: 1   MAFTAFLLLLSSIAAGFLLLLRRTRYRRMGLPPGSLGLPLIGETFQLIGAYKTENPEPFI 60

Query: 65  NSKRARYGSFFKSHILGSPTVVSMDPEVNRYILMNESKGLVAGYPQSMLDILGKCNIAAV 124
           + + ARYGS F +H+ G PT+ S DPE NR++L NE K     YP S+ ++LGK ++  +
Sbjct: 61  DERVARYGSVFMTHLFGEPTIFSADPETNRFVLQNEGKLFECSYPASICNLLGKHSLLLM 120

Query: 125 HGDAHKLMRGALLAIVSPTMIKDRLLLQVDEFMRSHLRNWDSQIIDIQEKTKQMAFWTSL 184
            G  HK M    ++  + ++IKD L+L +D  +R +L +W S+++ + E+ K++ F  ++
Sbjct: 121 KGSLHKRMHSLTMSFANSSIIKDHLMLDIDRLVRFNLDSWSSRVL-LMEEAKKITFELTV 180

Query: 185 KQTTSIESGPLWESFTIEFFKLVLGTFSLPIDLPGTNYRIGVQARNNIVKLLRQLINERG 244
           KQ  S + G   ES   E+  ++ G FSLP+ L  T YR  +QAR  + + L  ++ +R 
Sbjct: 181 KQLMSFDPGEWSESLRKEYLLVIEGFFSLPLPLFSTTYRKAIQARRKVAEALTVVVMKRR 240

Query: 245 NSKIQ----KDDMLGYLLSEENKYKLNEEQIIDQVITVFYSGYETVSTTSMMAVKFLHDH 304
             + +    K DML  LL+ ++ +  ++E+I+D ++ +  +GYET ST   +AVKFL + 
Sbjct: 241 EEEEEGAERKKDMLAALLAADDGF--SDEEIVDFLVALLVAGYETTSTIMTLAVKFLTET 300

Query: 305 PKVLQQLREEHLALREKKNPEDPIDWDDFKAMEFTRAVIFETSRLATVVNGVLRKTTKDL 364
           P  L QL+EEH  +R  K+    ++W D+K+M FT+ V+ ET R+A ++ GV R+   D+
Sbjct: 301 PLALAQLKEEHEKIRAMKSDSYSLEWSDYKSMPFTQCVVNETLRVANIIGGVFRRAMTDV 360

Query: 365 EVNGFLIPKGWRIYVYNREINYDPFLYSEPFTFNPWRWLDKSLES--HNYFFLFGGGIRL 424
           E+ G+ IPKGW+++   R ++ DP  + +  TFNPWRW   S+ +   N F  FGGG RL
Sbjct: 361 EIKGYKIPKGWKVFSSFRAVHLDPNHFKDARTFNPWRWQSNSVTTGPSNVFTPFGGGPRL 420

Query: 425 CPGKELGIAEVSTFLHYFVTKYRWEEVGECKFLKFPRVKARNGFHIRV 460
           CPG EL    +S FLH  VT + W    + K + FP  + +  + I V
Sbjct: 421 CPGYELARVALSVFLHRLVTGFSWVPAEQDKLVFFPTTRTQKRYPIFV 465

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q69F959.0e-19871.55Cytochrome P450 85A OS=Phaseolus vulgaris OX=3885 GN=BA13 PE=3 SV=2[more]
Q431471.1e-19268.75Cytochrome P450 85A1 OS=Solanum lycopersicum OX=4081 GN=CYP85A1 PE=2 SV=1[more]
Q940V41.4e-19068.17Cytochrome P450 85A2 OS=Arabidopsis thaliana OX=3702 GN=CYP85A2 PE=2 SV=1[more]
Q9FMA51.8e-18566.67Cytochrome P450 85A1 OS=Arabidopsis thaliana OX=3702 GN=CYP85A1 PE=2 SV=1[more]
Q50LE07.4e-17662.74Cytochrome P450 85A3 OS=Solanum lycopersicum OX=4081 GN=CYP85A3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_004150514.30.099.35cytochrome P450 85A [Cucumis sativus][more]
XP_008455791.10.094.59PREDICTED: cytochrome P450 85A-like [Cucumis melo][more]
KAE8649648.10.094.16hypothetical protein Csa_012605 [Cucumis sativus][more]
XP_038890036.12.82e-30186.80cytochrome P450 85A-like [Benincasa hispida][more]
KAA0040382.11.33e-29893.69cytochrome P450 85A-like [Cucumis melo var. makuwa] >TYK04049.1 cytochrome P450 ... [more]
Match NameE-valueIdentityDescription
A0A0A0L0Q50.099.13Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G410870 PE=3 SV=1[more]
A0A1S3C1A80.094.59cytochrome P450 85A-like OS=Cucumis melo OX=3656 GN=LOC103495886 PE=3 SV=1[more]
A0A5A7TA976.44e-29993.69Cytochrome P450 85A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A0A0KXL91.01e-29484.88Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G641590 PE=3 SV=1[more]
A0A5A7TDB41.18e-29384.67Cytochrome P450 85A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol... [more]
Match NameE-valueIdentityDescription
AT3G30180.19.9e-19268.17brassinosteroid-6-oxidase 2 [more]
AT5G38970.11.2e-18666.67brassinosteroid-6-oxidase 1 [more]
AT5G38970.37.4e-15566.58brassinosteroid-6-oxidase 1 [more]
AT5G38970.21.4e-15066.15brassinosteroid-6-oxidase 1 [more]
AT5G05690.16.4e-9036.97Cytochrome P450 superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 412..435
score: 24.43
coord: 61..80
score: 28.18
coord: 369..393
score: 29.19
coord: 402..412
score: 45.87
coord: 329..347
score: 30.77
IPR001128Cytochrome P450PRINTSPR00385P450coord: 274..291
score: 29.56
coord: 330..341
score: 41.2
coord: 403..412
score: 47.0
coord: 412..423
score: 32.17
IPR001128Cytochrome P450PFAMPF00067p450coord: 34..437
e-value: 5.1E-54
score: 183.8
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 24..461
e-value: 3.2E-95
score: 321.5
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 27..459
NoneNo IPR availablePIRSRPIRSR000047-1PIRSR000047-1coord: 5..438
e-value: 3.9E-17
score: 59.4
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 5..460
NoneNo IPR availablePANTHERPTHR24286:SF273CYTOCHROME P450 FAMILY PROTEINcoord: 5..460
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 405..414

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy4G017590.1CsGy4G017590.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016132 brassinosteroid biosynthetic process
biological_process GO:0010268 brassinosteroid homeostasis
biological_process GO:0016125 sterol metabolic process
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen