CsGy4G017510 (gene) Cucumber (Gy14) v2.1

Overview


Name	CsGy4G017510
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description	delta(7)-sterol-C5(6)-desaturase-like
Location	Gy14Chr4: 22632447 .. 22635707 (+)
RNA-Seq Expression	CsGy4G017510
Synteny	CsGy4G017510

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATAAATTCTCACAACTCATTTCAATTCCAACTCAGACTCTCTTTGTTTTTCCCTTTTTGCTCTTCTTCACTTCCTCTGGTTCTGATTTGACTGTTTCTTTTCTGTTTTCCGGTGGCTATTTTCGACAGCCATGGGCGGTGGGCCGCCGTATTTGGAACTCTTTGTTGAGGAGACTAGTTTCTACAACCGCATTGTTTTGGGAACCATTTTTCCAAACTTTAGTTTAGACCCATTTCCTCATATTCTTCAAACTTGGCTTCGAAACTGCGTTGGTGGATTCTTAATTTACTTCTTTTCTGGCTTTCTTTGGTGCTTTTACATCTATTACTGGAAGCGCAATGTCTATGTTCCCAAAGGTGTTGGCCTTTTTCTTCTTTCTTTTTCAGTTTGCTCTCTTTTGTGTTTTTTGCTGTTATTATCTCAACTGCTCCATCAATGAGTTCTAATTCTGGTTATTTGGGTTTTGTTATTTCCCTTACTTCTTTGTTGCCTCACTTTTTGATCTCTTAGTATTTCACTGATTATCCAACAGATTTGCGTGTCATTCCGGAGAATCTGGATTTGGTTTTCTTTCTACTAATTTCAGCCACTACTTCATGTTTGATTTAGCTATTATTCTTCAACTTATTCTTTTTTCTTTTGCTTATACGTGTGCTTACTGCATGCTATATCGGATTCATTTTGGTTCAATTGATTCATTTTGTTTTTGAATGTTGAGGTATTTTTTTTTTATTCTTCATTGATACTCTCTTCTTATGACACCATGGGATGTGCAAAAATAGTTTGTCTGGAAAGCTCTGTTTGAGATAAACTTAACATGACTTTGATGAGCCTTTTTACTTTTGAGATGCTGGTGATTTTTGGCTTTCCAGGATGGACGATTATAACGGATTACTAATCTATAGTAGTTTGAATCATCTACATGTTCTTGACAGATAGATTTCCTAAAAGACATGTTTTGATTTGTGCAAGCTGATGATTCTTCTTTCTTGTTTTTCATAAACAGATGAATTGTTCTGAAAAGAACTGTATATCCAACACTTAACCATAATAATTTAGAGCTAATAGATAGTATAGGATGTTGGGGCCTCTCAGTTAGGCTACTTAAAGAAGAGGATATTTCATTTGTTGCTCAAATGAAAGATTCTTCTATGCATGAACATAAATATTCAGGGAGAGGACATTGTATTTCCTTTAGTTTACATTTGTTACTTTTGGCTTAGAATATCAGTCACACCTATGATATTTGTGATTTAGATACTATTTATATAATGTGGTCTTTGAGAAACCTTGACTAATTTCAAGAAACAACTGCCTATTTTTATACACTTAGCGTTAATCAACTTGTGGGATGTTTTTGTAGATGGCTATTTTGAACTAAATATCATATGAGATGAATATTGAATATAGAGTCGGGCACACTATTCAATTGAAAGTTTTCTCAAGAGTAGCCTGTTTTTCTAATTTGATAGTTTTGTATGTATATTATATACCAATTTTTCATTTCAGGATAATTTGGTTTAGTATCATTGAATAGAATAAGCTTTTAAACTTAGAAATTATGTCGATCATCTGCCTCCGATCTGTTGTTTGATGAGTTGAGTACTTTTTCCCCCCAAAATTCACCAGCAGTTGAAGACGCGAGCTTTTGAAAATATACCTTGAACATTTATATGCATTATAATGTATAATAGTTTTCATTCATAGAGTATATTTAGGATTTGGACGAGGCTGTATGTAAGAATTCAAGTAACTCTTTTTAGACCTGCAATATCCTTCATGTAAAAAAAAATTCATATAATATGTATTGTATGGGTCAATGCTTGTATAATTGTTTGACCTGCTTGACACTTTCTTGCAGATTCTATTCCATCTAATAAATCGATGCTCTTGCAAATCCTTGTAACAATCAAGGCCATGCCATTGTACTGCGTCCTTCCAACTTTTGCTGAATATGTGGTTGAACATGATTGGACAAAATGCTATCCAAGGGTACTCGATGTCGGATGGCCTGCTTATGCCTTTCATATAATTACTTATCTTACCTTTATAGAGTTTTGTATCTATTGGATGCATAGGGGACTGCATGATATAAAGCCTCTTTACAAGTATCTTCATGCTAAACATCATATCTACAACAAGAAAATTACTCTCTCCCCCTTTGCTGGTAAGTTCTTAATCTGCTTTCTTAAATTTTGCAGCCACTTGCGATTCATCTTGCCATTCATGCCAAAAGATACTTGTTTGAAAAGATTGTATCTCATATCTGATTCTCGTTTGATTTGCTCAAGATCAGTTTGACTTTGTATCAAAACCCAAAATACGAACCAATCTCAATAAATCTCTTTCACTAATTGTCTTTTACAGGAAACAAACAAATGATTATCAAATGAAAAAAAGTGTCATGCTTTTCAATTCCATTGTGACGAATAGGAATACAGTTGACATATGACTATAGTTTGATTAGGAAGTTTACAATCCGAAAATAAACTGATGCTAAGGAAACTTCACTGGATTTTGAAGTAGTTGGGACAATGATCTGTTAAATTGCTTCATTTCCTGTTTGCTGCTTCTGACTTAGTACTAAAGTTCTTTGGATGTTTCTTGAAAGATTCTCTAGTGAATGGTCTTTTTGAATGGGCAGGTTTAGCATTTCACCCTATGGACGGGATATTGCAGGCAATACCTCATTTGTTTGCTTTATTTCTCATTCCAACCCATTTTAGAACACACATTGTGCTGCTATTCTTCGAGGTTGTATGGACAGCAAACATTCACGACGGCATCCACTCACGAATGTGGCCAGTTATGGGTGCTGGCTACCACACAATTCATCATACAAGATACCGCTACAATTATGGCCATTACTCCATATGGATGGATTGGATGTTTGGGACGCTCCTTGATCCAATGGATATAGAAGCCAAGGGGTCGTGATTGGTCATTGCTTTGACATCAACTGCTTAATTGTTATGGCGCTTGATGATTCTTTGTGTTCAATGTAAATCAAGTTAGTTAACGATTAGATCAGGTCTTCTAGTATGTTACAGAATAAGTTGTCATTCCAGGGTAATTTGTGAAGCCATACATACCATCACATCACACATTCATTCTCAGTGTCCAGGAATCCACAAGGCCTGTGTGCTGCTATTGATAATGGTAAATGGTAAGTAAAACTTGTGGAGGCATTAGTTATATCTTTGTATATCATATTGAAATGAAAACTTATGTGAAAAAAATAGCCAATGATCATGTATGGGG

mRNA sequence

ATAAATTCTCACAACTCATTTCAATTCCAACTCAGACTCTCTTTGTTTTTCCCTTTTTGCTCTTCTTCACTTCCTCTGGTTCTGATTTGACTGTTTCTTTTCTGTTTTCCGGTGGCTATTTTCGACAGCCATGGGCGGTGGGCCGCCGTATTTGGAACTCTTTGTTGAGGAGACTAGTTTCTACAACCGCATTGTTTTGGGAACCATTTTTCCAAACTTTAGTTTAGACCCATTTCCTCATATTCTTCAAACTTGGCTTCGAAACTGCGTTGGTGGATTCTTAATTTACTTCTTTTCTGGCTTTCTTTGGTGCTTTTACATCTATTACTGGAAGCGCAATGTCTATGTTCCCAAAGATTCTATTCCATCTAATAAATCGATGCTCTTGCAAATCCTTGTAACAATCAAGGCCATGCCATTGTACTGCGTCCTTCCAACTTTTGCTGAATATGTGGTTGAACATGATTGGACAAAATGCTATCCAAGGGTACTCGATGTCGGATGGCCTGCTTATGCCTTTCATATAATTACTTATCTTACCTTTATAGAGTTTTGTATCTATTGGATGCATAGGGGACTGCATGATATAAAGCCTCTTTACAAGTATCTTCATGCTAAACATCATATCTACAACAAGAAAATTACTCTCTCCCCCTTTGCTGGTTTAGCATTTCACCCTATGGACGGGATATTGCAGGCAATACCTCATTTGTTTGCTTTATTTCTCATTCCAACCCATTTTAGAACACACATTGTGCTGCTATTCTTCGAGGTTGTATGGACAGCAAACATTCACGACGGCATCCACTCACGAATGTGGCCAGTTATGGGTGCTGGCTACCACACAATTCATCATACAAGATACCGCTACAATTATGGCCATTACTCCATATGGATGGATTGGATGTTTGGGACGCTCCTTGATCCAATGGATATAGAAGCCAAGGGGTCGTGATTGGTCATTGCTTTGACATCAACTGCTTAATTGTTATGGCGCTTGATGATTCTTTGTGTTCAATGTAAATCAAGTTAGTTAACGATTAGATCAGGTCTTCTAGTATGTTACAGAATAAGTTGTCATTCCAGGGTAATTTGTGAAGCCATACATACCATCACATCACACATTCATTCTCAGTGTCCAGGAATCCACAAGGCCTGTGTGCTGCTATTGATAATGGTAAATGGTAAGTAAAACTTGTGGAGGCATTAGTTATATCTTTGTATATCATATTGAAATGAAAACTTATGTGAAAAAAATAGCCAATGATCATGTATGGGG

Coding sequence (CDS)

ATGGGCGGTGGGCCGCCGTATTTGGAACTCTTTGTTGAGGAGACTAGTTTCTACAACCGCATTGTTTTGGGAACCATTTTTCCAAACTTTAGTTTAGACCCATTTCCTCATATTCTTCAAACTTGGCTTCGAAACTGCGTTGGTGGATTCTTAATTTACTTCTTTTCTGGCTTTCTTTGGTGCTTTTACATCTATTACTGGAAGCGCAATGTCTATGTTCCCAAAGATTCTATTCCATCTAATAAATCGATGCTCTTGCAAATCCTTGTAACAATCAAGGCCATGCCATTGTACTGCGTCCTTCCAACTTTTGCTGAATATGTGGTTGAACATGATTGGACAAAATGCTATCCAAGGGTACTCGATGTCGGATGGCCTGCTTATGCCTTTCATATAATTACTTATCTTACCTTTATAGAGTTTTGTATCTATTGGATGCATAGGGGACTGCATGATATAAAGCCTCTTTACAAGTATCTTCATGCTAAACATCATATCTACAACAAGAAAATTACTCTCTCCCCCTTTGCTGGTTTAGCATTTCACCCTATGGACGGGATATTGCAGGCAATACCTCATTTGTTTGCTTTATTTCTCATTCCAACCCATTTTAGAACACACATTGTGCTGCTATTCTTCGAGGTTGTATGGACAGCAAACATTCACGACGGCATCCACTCACGAATGTGGCCAGTTATGGGTGCTGGCTACCACACAATTCATCATACAAGATACCGCTACAATTATGGCCATTACTCCATATGGATGGATTGGATGTTTGGGACGCTCCTTGATCCAATGGATATAGAAGCCAAGGGGTCGTGA

Protein sequence

MGGGPPYLELFVEETSFYNRIVLGTIFPNFSLDPFPHILQTWLRNCVGGFLIYFFSGFLWCFYIYYWKRNVYVPKDSIPSNKSMLLQILVTIKAMPLYCVLPTFAEYVVEHDWTKCYPRVLDVGWPAYAFHIITYLTFIEFCIYWMHRGLHDIKPLYKYLHAKHHIYNKKITLSPFAGLAFHPMDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTANIHDGIHSRMWPVMGAGYHTIHHTRYRYNYGHYSIWMDWMFGTLLDPMDIEAKGS*

Homology

[Close]

BLAST of CsGy4G017510 vs. ExPASy Swiss-Prot
Match: Q9ZT29 (Delta(7)-sterol-C5(6)-desaturase OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 413.7 bits (1062), Expect = 1.6e-114
Identity = 183/266 (68.80%), Postives = 218/266 (81.95%), Query Frame = 0


Query: 7   YLELFVEETSFYNRIVLGTIFPNFSLDPFPHILQTWLRNCVGGFLIYFFSGFLWCFYIYY 66
           YL+ FVEETSFYNR+VLGT  P     P PH+LQ WLRN +GG L+YF SGFLWCFYIY+
Sbjct: 4   YLKQFVEETSFYNRLVLGTFMPESWWGPLPHMLQGWLRNYIGGVLLYFISGFLWCFYIYH 63

Query: 67  WKRNVYVPKDSIPSNKSMLLQILVTIKAMPLYCVLPTFAEYVVEHDWTKCYPRVLDVGWP 126
            KRNVY+PKD+IPSNK+MLLQI V +KAMP YC LP+ +EY++E+ WTKC+ R+ DVGW 
Sbjct: 64  LKRNVYIPKDAIPSNKAMLLQISVAMKAMPWYCALPSLSEYMIENGWTKCFARISDVGWL 123

Query: 127 AYAFHIITYLTFIEFCIYWMHRGLHDIKPLYKYLHAKHHIYNKKITLSPFAGLAFHPMDG 186
           +Y  +   YL  +EF IYWMH  LHDIKPLYKYLHA HHIYNK+ TLSPFAGLAFHP+DG
Sbjct: 124 SYVIYAAIYLVIVEFGIYWMHMELHDIKPLYKYLHATHHIYNKQNTLSPFAGLAFHPLDG 183

Query: 187 ILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTANIHDGIHSRMWPVMGAGYHTIHHTRYR 246
           ILQA+PH+ AL L+P HF THI L+F E +WTANIHD IH +++PVMGAGYHTIHH  YR
Sbjct: 184 ILQAVPHVVALLLVPMHFSTHIALIFLEALWTANIHDCIHGKVFPVMGAGYHTIHHRTYR 243

Query: 247 YNYGHYSIWMDWMFGTLLDPMDIEAK 273
           +NYGHY+IWMDWMFGTL DP++ +AK
Sbjct: 244 HNYGHYTIWMDWMFGTLRDPVEEDAK 269

[Close]

BLAST of CsGy4G017510 vs. ExPASy Swiss-Prot
Match: Q39208 (Delta(7)-sterol-C5(6)-desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=STE1 PE=1 SV=2)

HSP 1 Score: 390.6 bits (1002), Expect = 1.5e-107
Identity = 176/267 (65.92%), Postives = 209/267 (78.28%), Query Frame = 0


Query: 1   MGGGPPYLELFVEETSFYNRIVLGTIFPNFSLDPFPHILQTWLRNCVGGFLIYFFSGFLW 60
           M     YL  FV+ETSFYNRIVL  + P    +P PH LQTWLRN + G L+YF SGFLW
Sbjct: 1   MAADNAYLMQFVDETSFYNRIVLSHLLPANLWEPLPHFLQTWLRNYLAGTLLYFISGFLW 60

Query: 61  CFYIYYWKRNVYVPKDSIPSNKSMLLQILVTIKAMPLYCVLPTFAEYVVEHDWTKCYPRV 120
           CFYIYY K NVY+PKD+IP+ K+M LQ+ V +KAMP Y +LPT +E ++E  WTKC+  +
Sbjct: 61  CFYIYYLKINVYLPKDAIPTIKAMRLQMFVAMKAMPWYTLLPTVSESMIERGWTKCFASI 120

Query: 121 LDVGWPAYAFHIITYLTFIEFCIYWMHRGLHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180
            + GW  Y  +I  YL F+EF IYWMHR LHDIKPLYKYLHA HHIYNK+ TLSPFAGLA
Sbjct: 121 GEFGWILYFVYIAIYLVFVEFGIYWMHRELHDIKPLYKYLHATHHIYNKQNTLSPFAGLA 180

Query: 181 FHPMDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTANIHDGIHSRMWPVMGAGYHTI 240
           FHP+DGILQA+PH+ ALF++P HF THI LLF E +WTANIHD IH  +WPVMGAGYHTI
Sbjct: 181 FHPVDGILQAVPHVIALFIVPIHFTTHIGLLFMEAIWTANIHDCIHGNIWPVMGAGYHTI 240

Query: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPM 268
           HHT Y++NYGHY+IWMDWMFG+L DP+
Sbjct: 241 HHTTYKHNYGHYTIWMDWMFGSLRDPL 267

[Close]

BLAST of CsGy4G017510 vs. ExPASy Swiss-Prot
Match: Q9M883 (Putative Delta(7)-sterol-C5(6)-desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=HDF7 PE=3 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 1.8e-100
Identity = 164/261 (62.84%), Postives = 202/261 (77.39%), Query Frame = 0


Query: 7   YLELFVEETSFYNRIVLGTIFPNFSLDPFPHILQTWLRNCVGGFLIYFFSGFLWCFYIYY 66
           Y +  V ETSFYNR+VL  + P    +P PH LQTWLRN + G ++YF SGFLWCFYIYY
Sbjct: 8   YNDQIVNETSFYNRMVLSHLLPVNLWEPLPHFLQTWLRNYLAGNILYFISGFLWCFYIYY 67

Query: 67  WKRNVYVPKDSIPSNKSMLLQILVTIKAMPLYCVLPTFAEYVVEHDWTKCYPRVLDVGWP 126
            K NVYVPK+SIP+ K+MLLQI V +KAMP Y +LP  +EY++EH WTKCY  +    W 
Sbjct: 68  LKLNVYVPKESIPTRKAMLLQIYVAMKAMPWYTLLPAVSEYMIEHGWTKCYSTLDHFNWF 127

Query: 127 AYAFHIITYLTFIEFCIYWMHRGLHDIKPLYKYLHAKHHIYNKKITLSPFAGLAFHPMDG 186
               +I  YL  +EF IYW+H+ LHDIK LYK+LHA HH+YNK+ TLSPFAGLAFHP+DG
Sbjct: 128 LCFLYIALYLVLVEFMIYWVHKELHDIKFLYKHLHATHHMYNKQNTLSPFAGLAFHPLDG 187

Query: 187 ILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTANIHDGIHSRMWPVMGAGYHTIHHTRYR 246
           ILQAIPH+ ALF++P H  TH+ LLF E +WTA+IHD IH  +WP+MGAGYHTIHHT Y+
Sbjct: 188 ILQAIPHVIALFIVPIHLITHLSLLFLEGIWTASIHDCIHGNIWPIMGAGYHTIHHTTYK 247

Query: 247 YNYGHYSIWMDWMFGTLLDPM 268
           +NYGHY+IWMDWMFG+L+ P+
Sbjct: 248 HNYGHYTIWMDWMFGSLMVPL 268

[Close]

BLAST of CsGy4G017510 vs. ExPASy Swiss-Prot
Match: Q9EQS5 (Lathosterol oxidase OS=Rattus norvegicus OX=10116 GN=Sc5d PE=1 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 4.4e-27
Identity = 79/234 (33.76%), Postives = 128/234 (54.70%), Query Frame = 0


Query: 47  VGGFLIYFFSGFLWCFYIYYWKRNVYVPKDSIPSNKSMLLQILVTIKAMPLYCVLPTFAE 106
           +G +++YFF   L  +++Y    +  + K        +  +I+ T+K++P +  +PT + 
Sbjct: 40  LGAYILYFFCATLSYYFVY----DHSLMKHPQFLKNQVSREIMFTVKSLP-WISIPTVSL 99

Query: 107 YVVE-HDWTKCYPRVLDV--GWPAYAFHIITYLTFIEFCIYWMHRGLHDIKPLYKYLHAK 166
           +++E   ++K Y  + D   GW      +I++L F +  IYW+HRGLH  + LYK++H  
Sbjct: 100 FLLELRGYSKLYDDIGDFPNGWIHLIMSVISFLFFTDMLIYWIHRGLHH-RLLYKHIHKP 159

Query: 167 HHIYNKKITLSPFAGLAFHPMDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTANIHD 226
           HHI+  KI  +PFA  AFHP+DG LQ++P+    F+ P H   ++ L     VWT +IHD
Sbjct: 160 HHIW--KIP-TPFASHAFHPVDGFLQSLPYHIYPFVFPLHKVVYLGLYVLVNVWTISIHD 219

Query: 227 G---IHSRMWP-VMGAGYHTIHHTRYRYNYGHYSIWMDWMFGTLLDPMDIEAKG 274
           G   +     P + G+ +HT HH  + YNYG Y    D + G+   P   E KG
Sbjct: 220 GDFRVPQIFRPFINGSAHHTDHHMLFDYNYGQYFTLWDRIGGSFKHPSSFEGKG 264

[Close]

BLAST of CsGy4G017510 vs. ExPASy Swiss-Prot
Match: O88822 (Lathosterol oxidase OS=Mus musculus OX=10090 GN=Sc5d PE=2 SV=2)

HSP 1 Score: 119.8 bits (299), Expect = 4.9e-26
Identity = 77/234 (32.91%), Postives = 128/234 (54.70%), Query Frame = 0


Query: 47  VGGFLIYFFSGFLWCFYIYYWKRNVYVPKDSIPSNKSMLLQILVTIKAMPLYCVLPTFAE 106
           +G +++YFF   L  +++Y    +  + K        +  +I+ T+K++P +  +PT + 
Sbjct: 40  LGAYILYFFCATLSYYFVY----DHSLMKHPQFLKNQVSREIVFTVKSLP-WISIPTVSL 99

Query: 107 YVVE-HDWTKCYPRVLDV--GWPAYAFHIITYLTFIEFCIYWMHRGLHDIKPLYKYLHAK 166
           +++E   ++K Y  + D   GW      ++++L F +  IYW+HRGLH  + +YK +H  
Sbjct: 100 FLLELRGYSKLYDDIGDFPNGWIHLMVSVVSFLFFTDMLIYWIHRGLHH-RLVYKRIHKP 159

Query: 167 HHIYNKKITLSPFAGLAFHPMDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTANIHD 226
           HHI+  KI  +PFA  AFHP+DG LQ++P+    F+ P H   ++ L     VWT +IHD
Sbjct: 160 HHIW--KIP-TPFASHAFHPVDGFLQSLPYHIYPFVFPLHKVVYLGLYVLVNVWTISIHD 219

Query: 227 G---IHSRMWP-VMGAGYHTIHHTRYRYNYGHYSIWMDWMFGTLLDPMDIEAKG 274
           G   +   + P + G+ +HT HH  + YNYG Y    D + G+   P   E KG
Sbjct: 220 GDFRVPQILRPFINGSAHHTDHHMFFDYNYGQYFTLWDRIGGSFKHPSSFEGKG 264

[Close]

BLAST of CsGy4G017510 vs. NCBI nr
Match: XP_004150515.1 (delta(7)-sterol-C5(6)-desaturase [Cucumis sativus] >KGN54626.1 hypothetical protein Csa_012304 [Cucumis sativus])

HSP 1 Score: 597 bits (1538), Expect = 6.99e-216
Identity = 274/274 (100.00%), Postives = 274/274 (100.00%), Query Frame = 0


Query: 1   MGGGPPYLELFVEETSFYNRIVLGTIFPNFSLDPFPHILQTWLRNCVGGFLIYFFSGFLW 60
           MGGGPPYLELFVEETSFYNRIVLGTIFPNFSLDPFPHILQTWLRNCVGGFLIYFFSGFLW
Sbjct: 1   MGGGPPYLELFVEETSFYNRIVLGTIFPNFSLDPFPHILQTWLRNCVGGFLIYFFSGFLW 60

Query: 61  CFYIYYWKRNVYVPKDSIPSNKSMLLQILVTIKAMPLYCVLPTFAEYVVEHDWTKCYPRV 120
           CFYIYYWKRNVYVPKDSIPSNKSMLLQILVTIKAMPLYCVLPTFAEYVVEHDWTKCYPRV
Sbjct: 61  CFYIYYWKRNVYVPKDSIPSNKSMLLQILVTIKAMPLYCVLPTFAEYVVEHDWTKCYPRV 120

Query: 121 LDVGWPAYAFHIITYLTFIEFCIYWMHRGLHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180
           LDVGWPAYAFHIITYLTFIEFCIYWMHRGLHDIKPLYKYLHAKHHIYNKKITLSPFAGLA
Sbjct: 121 LDVGWPAYAFHIITYLTFIEFCIYWMHRGLHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180

Query: 181 FHPMDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTANIHDGIHSRMWPVMGAGYHTI 240
           FHPMDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTANIHDGIHSRMWPVMGAGYHTI
Sbjct: 181 FHPMDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTANIHDGIHSRMWPVMGAGYHTI 240

Query: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDIEAKGS 274
           HHTRYRYNYGHYSIWMDWMFGTLLDPMDIEAKGS
Sbjct: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDIEAKGS 274

[Close]

BLAST of CsGy4G017510 vs. NCBI nr
Match: XP_008444093.1 (PREDICTED: delta(7)-sterol-C5(6)-desaturase-like [Cucumis melo])

HSP 1 Score: 589 bits (1519), Expect = 5.52e-213
Identity = 271/273 (99.27%), Postives = 271/273 (99.27%), Query Frame = 0


Query: 1   MGGGPPYLELFVEETSFYNRIVLGTIFPNFSLDPFPHILQTWLRNCVGGFLIYFFSGFLW 60
           MGGGPPYLELFVEETSFYNRIVLGTIFPNFSL PFPHILQTWLRNCVGGFLIYFFSGFLW
Sbjct: 1   MGGGPPYLELFVEETSFYNRIVLGTIFPNFSLYPFPHILQTWLRNCVGGFLIYFFSGFLW 60

Query: 61  CFYIYYWKRNVYVPKDSIPSNKSMLLQILVTIKAMPLYCVLPTFAEYVVEHDWTKCYPRV 120
           CFYIYYWKRNVYVPKDSIPSNKSMLLQILVTIKAMPLYCVLPTFAEYVVEHDWTKCYPRV
Sbjct: 61  CFYIYYWKRNVYVPKDSIPSNKSMLLQILVTIKAMPLYCVLPTFAEYVVEHDWTKCYPRV 120

Query: 121 LDVGWPAYAFHIITYLTFIEFCIYWMHRGLHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180
           LDVGWPAYAFHIITYLTFIEFCIYWMHRGLHDIKPLYKYLHAKHHIYNKK TLSPFAGLA
Sbjct: 121 LDVGWPAYAFHIITYLTFIEFCIYWMHRGLHDIKPLYKYLHAKHHIYNKKTTLSPFAGLA 180

Query: 181 FHPMDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTANIHDGIHSRMWPVMGAGYHTI 240
           FHPMDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTANIHDGIHSRMWPVMGAGYHTI
Sbjct: 181 FHPMDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTANIHDGIHSRMWPVMGAGYHTI 240

Query: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDIEAKG 273
           HHTRYRYNYGHYSIWMDWMFGTLLDPMDIEAKG
Sbjct: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDIEAKG 273

[Close]

BLAST of CsGy4G017510 vs. NCBI nr
Match: XP_038894105.1 (delta(7)-sterol-C5(6)-desaturase-like [Benincasa hispida] >XP_038894106.1 delta(7)-sterol-C5(6)-desaturase-like [Benincasa hispida] >XP_038894107.1 delta(7)-sterol-C5(6)-desaturase-like [Benincasa hispida])

HSP 1 Score: 565 bits (1456), Expect = 2.23e-203
Identity = 253/274 (92.34%), Postives = 264/274 (96.35%), Query Frame = 0


Query: 1   MGGGPPYLELFVEETSFYNRIVLGTIFPNFSLDPFPHILQTWLRNCVGGFLIYFFSGFLW 60
           MGGGPPYLELFVEET FYNRIVLGTIFP F+LDPFPHI+QTWLRNC+GGFLIYFFS FLW
Sbjct: 1   MGGGPPYLELFVEETDFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGFLIYFFSAFLW 60

Query: 61  CFYIYYWKRNVYVPKDSIPSNKSMLLQILVTIKAMPLYCVLPTFAEYVVEHDWTKCYPRV 120
           CFYIYYWKRNVY+PKDSIPSNKSMLLQI VTIKAMPLYC+LPTF+EYVVEHDWTKCYPRV
Sbjct: 61  CFYIYYWKRNVYIPKDSIPSNKSMLLQIFVTIKAMPLYCILPTFSEYVVEHDWTKCYPRV 120

Query: 121 LDVGWPAYAFHIITYLTFIEFCIYWMHRGLHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180
           LDVGWPAYAFH+ITYL FIEF IYWMHRGLHDIKPLYKYLHAKHHIYNKKITLSPFAGLA
Sbjct: 121 LDVGWPAYAFHVITYLAFIEFSIYWMHRGLHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180

Query: 181 FHPMDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTANIHDGIHSRMWPVMGAGYHTI 240
           FHP+DGILQAIPHLFALFLIPTHFRTHIVLLFFEV+WT NIHD IH+RMWPVMGAGYHTI
Sbjct: 181 FHPIDGILQAIPHLFALFLIPTHFRTHIVLLFFEVIWTTNIHDCIHARMWPVMGAGYHTI 240

Query: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDIEAKGS 274
           HHTRYRYNYGHYSIWMDWMFGTL+DPMD E KGS
Sbjct: 241 HHTRYRYNYGHYSIWMDWMFGTLIDPMDTEGKGS 274

[Close]

BLAST of CsGy4G017510 vs. NCBI nr
Match: XP_022980690.1 (delta(7)-sterol-C5(6)-desaturase-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 551 bits (1419), Expect = 9.79e-198
Identity = 247/274 (90.15%), Postives = 260/274 (94.89%), Query Frame = 0


Query: 1   MGGGPPYLELFVEETSFYNRIVLGTIFPNFSLDPFPHILQTWLRNCVGGFLIYFFSGFLW 60
           MGGG PYLELFV ETSFYNRIVLGTIFP F+LDPFPHI+QTWLRNC+GG LIYFFSGFLW
Sbjct: 1   MGGGQPYLELFVAETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGLLIYFFSGFLW 60

Query: 61  CFYIYYWKRNVYVPKDSIPSNKSMLLQILVTIKAMPLYCVLPTFAEYVVEHDWTKCYPRV 120
           CFYIYYWKRNVYVPKDSIPSNK ML Q+ VTIKAMP YC+LPTFAEYVVEHDWTKCYPRV
Sbjct: 61  CFYIYYWKRNVYVPKDSIPSNKLMLFQMFVTIKAMPFYCILPTFAEYVVEHDWTKCYPRV 120

Query: 121 LDVGWPAYAFHIITYLTFIEFCIYWMHRGLHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180
           LDVGW AYAFHI+TYL F+EFCIYWMHR LHDIKPLYKYLHAKHHIYNKKITLSPFAGLA
Sbjct: 121 LDVGWRAYAFHIVTYLAFVEFCIYWMHRALHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180

Query: 181 FHPMDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTANIHDGIHSRMWPVMGAGYHTI 240
           FHP+DGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWT NIHD IH+++WPVMGAGYHTI
Sbjct: 181 FHPVDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTTNIHDCIHAQIWPVMGAGYHTI 240

Query: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDIEAKGS 274
           HHTRYRYNYGHYSIWMDWMFGTLLDP+D EA+GS
Sbjct: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPIDTEARGS 274

[Close]

BLAST of CsGy4G017510 vs. NCBI nr
Match: XP_022956224.1 (delta(7)-sterol-C5(6)-desaturase-like [Cucurbita moschata] >KAG6581752.1 hypothetical protein SDJN03_21754, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026198.1 hypothetical protein SDJN02_12697, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 549 bits (1415), Expect = 3.99e-197
Identity = 245/274 (89.42%), Postives = 260/274 (94.89%), Query Frame = 0


Query: 1   MGGGPPYLELFVEETSFYNRIVLGTIFPNFSLDPFPHILQTWLRNCVGGFLIYFFSGFLW 60
           MGGG PYLELFV ETSFYNRIVLGTIFP F+LDPFPHI+QTWLRNC+GG LIYFFSGFLW
Sbjct: 1   MGGGQPYLELFVAETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGLLIYFFSGFLW 60

Query: 61  CFYIYYWKRNVYVPKDSIPSNKSMLLQILVTIKAMPLYCVLPTFAEYVVEHDWTKCYPRV 120
           CFYIYYWKRNVYVPKDSIPSNK ML Q+ VTIKAMP YC+LPTFAEYVVEHDWTKCYPRV
Sbjct: 61  CFYIYYWKRNVYVPKDSIPSNKLMLFQMFVTIKAMPFYCILPTFAEYVVEHDWTKCYPRV 120

Query: 121 LDVGWPAYAFHIITYLTFIEFCIYWMHRGLHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180
           LDVGW AYAFHI+TYL F+EFCIYWMHR LHDIKPLYKYLHAKHHIYNKKITLSPFAGLA
Sbjct: 121 LDVGWRAYAFHIVTYLAFVEFCIYWMHRALHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180

Query: 181 FHPMDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTANIHDGIHSRMWPVMGAGYHTI 240
           FHP+DGILQAIPHLFALFLIPTHFRTH++LLFFEVVWT NIHD IH+++WPVMGAGYHTI
Sbjct: 181 FHPVDGILQAIPHLFALFLIPTHFRTHVMLLFFEVVWTTNIHDCIHAQIWPVMGAGYHTI 240

Query: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDIEAKGS 274
           HHTRYRYNYGHYSIWMDWMFGTLLDP+D EA+GS
Sbjct: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPIDTEARGS 274

[Close]

BLAST of CsGy4G017510 vs. ExPASy TrEMBL
Match: A0A0A0L3B9 (Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G409800 PE=3 SV=1)

HSP 1 Score: 597 bits (1538), Expect = 3.38e-216
Identity = 274/274 (100.00%), Postives = 274/274 (100.00%), Query Frame = 0


Query: 1   MGGGPPYLELFVEETSFYNRIVLGTIFPNFSLDPFPHILQTWLRNCVGGFLIYFFSGFLW 60
           MGGGPPYLELFVEETSFYNRIVLGTIFPNFSLDPFPHILQTWLRNCVGGFLIYFFSGFLW
Sbjct: 1   MGGGPPYLELFVEETSFYNRIVLGTIFPNFSLDPFPHILQTWLRNCVGGFLIYFFSGFLW 60

Query: 61  CFYIYYWKRNVYVPKDSIPSNKSMLLQILVTIKAMPLYCVLPTFAEYVVEHDWTKCYPRV 120
           CFYIYYWKRNVYVPKDSIPSNKSMLLQILVTIKAMPLYCVLPTFAEYVVEHDWTKCYPRV
Sbjct: 61  CFYIYYWKRNVYVPKDSIPSNKSMLLQILVTIKAMPLYCVLPTFAEYVVEHDWTKCYPRV 120

Query: 121 LDVGWPAYAFHIITYLTFIEFCIYWMHRGLHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180
           LDVGWPAYAFHIITYLTFIEFCIYWMHRGLHDIKPLYKYLHAKHHIYNKKITLSPFAGLA
Sbjct: 121 LDVGWPAYAFHIITYLTFIEFCIYWMHRGLHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180

Query: 181 FHPMDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTANIHDGIHSRMWPVMGAGYHTI 240
           FHPMDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTANIHDGIHSRMWPVMGAGYHTI
Sbjct: 181 FHPMDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTANIHDGIHSRMWPVMGAGYHTI 240

Query: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDIEAKGS 274
           HHTRYRYNYGHYSIWMDWMFGTLLDPMDIEAKGS
Sbjct: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDIEAKGS 274

[Close]

BLAST of CsGy4G017510 vs. ExPASy TrEMBL
Match: A0A1S3BAC7 (delta(7)-sterol-C5(6)-desaturase-like OS=Cucumis melo OX=3656 GN=LOC103487532 PE=3 SV=1)

HSP 1 Score: 589 bits (1519), Expect = 2.67e-213
Identity = 271/273 (99.27%), Postives = 271/273 (99.27%), Query Frame = 0


Query: 1   MGGGPPYLELFVEETSFYNRIVLGTIFPNFSLDPFPHILQTWLRNCVGGFLIYFFSGFLW 60
           MGGGPPYLELFVEETSFYNRIVLGTIFPNFSL PFPHILQTWLRNCVGGFLIYFFSGFLW
Sbjct: 1   MGGGPPYLELFVEETSFYNRIVLGTIFPNFSLYPFPHILQTWLRNCVGGFLIYFFSGFLW 60

Query: 61  CFYIYYWKRNVYVPKDSIPSNKSMLLQILVTIKAMPLYCVLPTFAEYVVEHDWTKCYPRV 120
           CFYIYYWKRNVYVPKDSIPSNKSMLLQILVTIKAMPLYCVLPTFAEYVVEHDWTKCYPRV
Sbjct: 61  CFYIYYWKRNVYVPKDSIPSNKSMLLQILVTIKAMPLYCVLPTFAEYVVEHDWTKCYPRV 120

Query: 121 LDVGWPAYAFHIITYLTFIEFCIYWMHRGLHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180
           LDVGWPAYAFHIITYLTFIEFCIYWMHRGLHDIKPLYKYLHAKHHIYNKK TLSPFAGLA
Sbjct: 121 LDVGWPAYAFHIITYLTFIEFCIYWMHRGLHDIKPLYKYLHAKHHIYNKKTTLSPFAGLA 180

Query: 181 FHPMDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTANIHDGIHSRMWPVMGAGYHTI 240
           FHPMDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTANIHDGIHSRMWPVMGAGYHTI
Sbjct: 181 FHPMDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTANIHDGIHSRMWPVMGAGYHTI 240

Query: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDIEAKG 273
           HHTRYRYNYGHYSIWMDWMFGTLLDPMDIEAKG
Sbjct: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDIEAKG 273

[Close]

BLAST of CsGy4G017510 vs. ExPASy TrEMBL
Match: A0A6J1IX84 (delta(7)-sterol-C5(6)-desaturase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111479986 PE=3 SV=1)

HSP 1 Score: 551 bits (1419), Expect = 4.74e-198
Identity = 247/274 (90.15%), Postives = 260/274 (94.89%), Query Frame = 0


Query: 1   MGGGPPYLELFVEETSFYNRIVLGTIFPNFSLDPFPHILQTWLRNCVGGFLIYFFSGFLW 60
           MGGG PYLELFV ETSFYNRIVLGTIFP F+LDPFPHI+QTWLRNC+GG LIYFFSGFLW
Sbjct: 1   MGGGQPYLELFVAETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGLLIYFFSGFLW 60

Query: 61  CFYIYYWKRNVYVPKDSIPSNKSMLLQILVTIKAMPLYCVLPTFAEYVVEHDWTKCYPRV 120
           CFYIYYWKRNVYVPKDSIPSNK ML Q+ VTIKAMP YC+LPTFAEYVVEHDWTKCYPRV
Sbjct: 61  CFYIYYWKRNVYVPKDSIPSNKLMLFQMFVTIKAMPFYCILPTFAEYVVEHDWTKCYPRV 120

Query: 121 LDVGWPAYAFHIITYLTFIEFCIYWMHRGLHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180
           LDVGW AYAFHI+TYL F+EFCIYWMHR LHDIKPLYKYLHAKHHIYNKKITLSPFAGLA
Sbjct: 121 LDVGWRAYAFHIVTYLAFVEFCIYWMHRALHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180

Query: 181 FHPMDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTANIHDGIHSRMWPVMGAGYHTI 240
           FHP+DGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWT NIHD IH+++WPVMGAGYHTI
Sbjct: 181 FHPVDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTTNIHDCIHAQIWPVMGAGYHTI 240

Query: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDIEAKGS 274
           HHTRYRYNYGHYSIWMDWMFGTLLDP+D EA+GS
Sbjct: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPIDTEARGS 274

[Close]

BLAST of CsGy4G017510 vs. ExPASy TrEMBL
Match: A0A6J1GYF7 (delta(7)-sterol-C5(6)-desaturase-like OS=Cucurbita moschata OX=3662 GN=LOC111457984 PE=3 SV=1)

HSP 1 Score: 549 bits (1415), Expect = 1.93e-197
Identity = 245/274 (89.42%), Postives = 260/274 (94.89%), Query Frame = 0


Query: 1   MGGGPPYLELFVEETSFYNRIVLGTIFPNFSLDPFPHILQTWLRNCVGGFLIYFFSGFLW 60
           MGGG PYLELFV ETSFYNRIVLGTIFP F+LDPFPHI+QTWLRNC+GG LIYFFSGFLW
Sbjct: 1   MGGGQPYLELFVAETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGLLIYFFSGFLW 60

Query: 61  CFYIYYWKRNVYVPKDSIPSNKSMLLQILVTIKAMPLYCVLPTFAEYVVEHDWTKCYPRV 120
           CFYIYYWKRNVYVPKDSIPSNK ML Q+ VTIKAMP YC+LPTFAEYVVEHDWTKCYPRV
Sbjct: 61  CFYIYYWKRNVYVPKDSIPSNKLMLFQMFVTIKAMPFYCILPTFAEYVVEHDWTKCYPRV 120

Query: 121 LDVGWPAYAFHIITYLTFIEFCIYWMHRGLHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180
           LDVGW AYAFHI+TYL F+EFCIYWMHR LHDIKPLYKYLHAKHHIYNKKITLSPFAGLA
Sbjct: 121 LDVGWRAYAFHIVTYLAFVEFCIYWMHRALHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180

Query: 181 FHPMDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTANIHDGIHSRMWPVMGAGYHTI 240
           FHP+DGILQAIPHLFALFLIPTHFRTH++LLFFEVVWT NIHD IH+++WPVMGAGYHTI
Sbjct: 181 FHPVDGILQAIPHLFALFLIPTHFRTHVMLLFFEVVWTTNIHDCIHAQIWPVMGAGYHTI 240

Query: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDIEAKGS 274
           HHTRYRYNYGHYSIWMDWMFGTLLDP+D EA+GS
Sbjct: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPIDTEARGS 274

[Close]

BLAST of CsGy4G017510 vs. ExPASy TrEMBL
Match: A0A6J1KDR9 (delta(7)-sterol-C5(6)-desaturase-like OS=Cucurbita maxima OX=3661 GN=LOC111492934 PE=3 SV=1)

HSP 1 Score: 534 bits (1376), Expect = 1.70e-191
Identity = 240/274 (87.59%), Postives = 256/274 (93.43%), Query Frame = 0


Query: 1   MGGGPPYLELFVEETSFYNRIVLGTIFPNFSLDPFPHILQTWLRNCVGGFLIYFFSGFLW 60
           MGG PPYLELF+EETS YNRIVLGTIFP+F LDPFPHILQTWLRNC+GGFLIYFFSGFLW
Sbjct: 1   MGGRPPYLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLW 60

Query: 61  CFYIYYWKRNVYVPKDSIPSNKSMLLQILVTIKAMPLYCVLPTFAEYVVEHDWTKCYPRV 120
           CF+IYYWKRNVYVPKDSIPSNK MLLQI  TIKAMPLYCVLPTF+EYVVEHDWT+CYPRV
Sbjct: 61  CFFIYYWKRNVYVPKDSIPSNKLMLLQIHATIKAMPLYCVLPTFSEYVVEHDWTRCYPRV 120

Query: 121 LDVGWPAYAFHIITYLTFIEFCIYWMHRGLHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180
            DVGW AYAFH+ TYLTF+EF IYWMHR LHD+KPLYKYLHAKHH+YNKK TLSPFAGLA
Sbjct: 121 FDVGWLAYAFHVTTYLTFVEFFIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLA 180

Query: 181 FHPMDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTANIHDGIHSRMWPVMGAGYHTI 240
           FHP+DGILQAIPHLFALFLIP HFRTHI+LLFFEVVWT NIHD IH++MWPVMGAGYHT+
Sbjct: 181 FHPVDGILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTL 240

Query: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDIEAKGS 274
           HHTRYRYNYGHYSIWMDWMFGTLLDPMD + K S
Sbjct: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDTQHKLS 274

[Close]

BLAST of CsGy4G017510 vs. TAIR 10
Match: AT3G02580.1 (sterol 1 )

HSP 1 Score: 390.6 bits (1002), Expect = 1.1e-108
Identity = 176/267 (65.92%), Postives = 209/267 (78.28%), Query Frame = 0


Query: 1   MGGGPPYLELFVEETSFYNRIVLGTIFPNFSLDPFPHILQTWLRNCVGGFLIYFFSGFLW 60
           M     YL  FV+ETSFYNRIVL  + P    +P PH LQTWLRN + G L+YF SGFLW
Sbjct: 1   MAADNAYLMQFVDETSFYNRIVLSHLLPANLWEPLPHFLQTWLRNYLAGTLLYFISGFLW 60

Query: 61  CFYIYYWKRNVYVPKDSIPSNKSMLLQILVTIKAMPLYCVLPTFAEYVVEHDWTKCYPRV 120
           CFYIYY K NVY+PKD+IP+ K+M LQ+ V +KAMP Y +LPT +E ++E  WTKC+  +
Sbjct: 61  CFYIYYLKINVYLPKDAIPTIKAMRLQMFVAMKAMPWYTLLPTVSESMIERGWTKCFASI 120

Query: 121 LDVGWPAYAFHIITYLTFIEFCIYWMHRGLHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180
            + GW  Y  +I  YL F+EF IYWMHR LHDIKPLYKYLHA HHIYNK+ TLSPFAGLA
Sbjct: 121 GEFGWILYFVYIAIYLVFVEFGIYWMHRELHDIKPLYKYLHATHHIYNKQNTLSPFAGLA 180

Query: 181 FHPMDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTANIHDGIHSRMWPVMGAGYHTI 240
           FHP+DGILQA+PH+ ALF++P HF THI LLF E +WTANIHD IH  +WPVMGAGYHTI
Sbjct: 181 FHPVDGILQAVPHVIALFIVPIHFTTHIGLLFMEAIWTANIHDCIHGNIWPVMGAGYHTI 240

Query: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPM 268
           HHT Y++NYGHY+IWMDWMFG+L DP+
Sbjct: 241 HHTTYKHNYGHYTIWMDWMFGSLRDPL 267

[Close]

BLAST of CsGy4G017510 vs. TAIR 10
Match: AT3G02590.1 (Fatty acid hydroxylase superfamily protein )

HSP 1 Score: 367.1 bits (941), Expect = 1.3e-101
Identity = 164/261 (62.84%), Postives = 202/261 (77.39%), Query Frame = 0


Query: 7   YLELFVEETSFYNRIVLGTIFPNFSLDPFPHILQTWLRNCVGGFLIYFFSGFLWCFYIYY 66
           Y +  V ETSFYNR+VL  + P    +P PH LQTWLRN + G ++YF SGFLWCFYIYY
Sbjct: 8   YNDQIVNETSFYNRMVLSHLLPVNLWEPLPHFLQTWLRNYLAGNILYFISGFLWCFYIYY 67

Query: 67  WKRNVYVPKDSIPSNKSMLLQILVTIKAMPLYCVLPTFAEYVVEHDWTKCYPRVLDVGWP 126
            K NVYVPK+SIP+ K+MLLQI V +KAMP Y +LP  +EY++EH WTKCY  +    W 
Sbjct: 68  LKLNVYVPKESIPTRKAMLLQIYVAMKAMPWYTLLPAVSEYMIEHGWTKCYSTLDHFNWF 127

Query: 127 AYAFHIITYLTFIEFCIYWMHRGLHDIKPLYKYLHAKHHIYNKKITLSPFAGLAFHPMDG 186
               +I  YL  +EF IYW+H+ LHDIK LYK+LHA HH+YNK+ TLSPFAGLAFHP+DG
Sbjct: 128 LCFLYIALYLVLVEFMIYWVHKELHDIKFLYKHLHATHHMYNKQNTLSPFAGLAFHPLDG 187

Query: 187 ILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTANIHDGIHSRMWPVMGAGYHTIHHTRYR 246
           ILQAIPH+ ALF++P H  TH+ LLF E +WTA+IHD IH  +WP+MGAGYHTIHHT Y+
Sbjct: 188 ILQAIPHVIALFIVPIHLITHLSLLFLEGIWTASIHDCIHGNIWPIMGAGYHTIHHTTYK 247

Query: 247 YNYGHYSIWMDWMFGTLLDPM 268
           +NYGHY+IWMDWMFG+L+ P+
Sbjct: 248 HNYGHYTIWMDWMFGSLMVPL 268

[Close]

BLAST of CsGy4G017510 vs. TAIR 10
Match: AT1G69640.1 (sphingoid base hydroxylase 1 )

HSP 1 Score: 51.2 bits (121), Expect = 1.5e-06
Identity = 42/154 (27.27%), Postives = 72/154 (46.75%), Query Frame = 0


Query: 129 AFHIITYLTFIEFCIYWMHRGLHDIKPLYKYLHAKHHIYNKKITLSPFAGLAFHPMDG-I 188
           A   +T +  ++   Y+MHR +H  K LYK++H++HH   + I    +  L  HP++G +
Sbjct: 96  ARQFVTAMIVLDTWQYFMHRYMHQNKFLYKHIHSQHH---RLIVPYAYGALYNHPVEGLL 155

Query: 189 LQAIPHLFALFLIPTHFRTHIVLLFFEVVWTANIHDGIH-----SRMWPVMGAGYHTIHH 248
           L  I    +  +     RT I    F  + T + H G+        M     + YH IHH
Sbjct: 156 LDTIGGALSFLVSGMSPRTSIFFFSFATIKTVDDHCGLWLPGNLFHMVFKNNSAYHDIHH 215

Query: 249 ----TRYRYNYGHYSIWMDWMFGTLLDPMDIEAK 273
               T+Y ++   + +W D + GT + P  +E +
Sbjct: 216 QLYGTKYNFSQPFFVMW-DRILGTYM-PYSLEKR 244

[Close]

BLAST of CsGy4G017510 vs. TAIR 10
Match: AT1G14290.1 (sphingoid base hydroxylase 2 )

HSP 1 Score: 48.1 bits (113), Expect = 1.3e-05
Identity = 42/156 (26.92%), Postives = 71/156 (45.51%), Query Frame = 0


Query: 129 AFHIITYLTFIEFCIYWMHRGLHDIKPLYKYLHAKHHIYNKKITLSPFAGLAFHPMDG-I 188
           A   I  +  I+   Y++HR +H  K LYK++H++HH   + I    +  L  HP++G +
Sbjct: 95  ARQFIIAMLVIDTWQYFIHRYMHLNKFLYKHIHSQHH---RLIVPYSYGALYNHPLEGLL 154

Query: 189 LQAIPHLFALFLIPTHFRTHIVLLFFEVVWTANIHDGIHSRMWPV-----MGAGYHTIHH 248
           L  I    +        RT I    F  + T + H G+     P        + YH +HH
Sbjct: 155 LDTIGGALSFLFSGMSPRTAIFFFSFATIKTVDDHCGLWLPGNPFHIFFSNNSAYHDVHH 214

Query: 249 ----TRYRYNYGHYSIWMDWMFGTLLDPMDIEAKGS 275
               T+Y ++   + +W D + GT L P  +E + +
Sbjct: 215 QLYGTKYNFSQPFFVMW-DRILGTYL-PYSLEKRAN 245

[Close]

BLAST of CsGy4G017510 vs. TAIR 10
Match: AT1G02205.2 (Fatty acid hydroxylase superfamily )

HSP 1 Score: 48.1 bits (113), Expect = 1.3e-05
Identity = 45/151 (29.80%), Postives = 70/151 (46.36%), Query Frame = 0


Query: 139 IEFCIYWMHRGLHDIKPLYKYLHAKHH--IYNKKIT--LSPFAGLAFHPMDGILQAIPHL 198
           +EF  YW+H+ LH    LY   H+ HH  I  + IT  + PFA    H    IL AIP L
Sbjct: 138 VEFLYYWLHKALHH-HFLYSRYHSHHHSSIVTEPITSVIHPFAE---HIAYFILFAIPLL 197

Query: 199 ------------FALFLIPTHFRTHIVLLFFEVVWTANIHDGIHSRMWPVM-----GAGY 258
                       FA ++I   F  ++    FE++     H      ++P +        Y
Sbjct: 198 TTLLTKTASIISFAGYIIYIDFMNNMGHCNFELIPKRLFH------LFPPLKFLCYTPSY 257

Query: 259 HTIHHTRYRYNYGHYSIWMDWMFGTLLDPMD 269
           H++HHT++R NY  +    D+++GT+ +  D
Sbjct: 258 HSLHHTQFRTNYSLFMPLYDYIYGTMDESTD 278

The following BLAST results are available for this feature:

BLAST of CsGy4G017510 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZT29	1.6e-114	68.80	Delta(7)-sterol-C5(6)-desaturase OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
Q39208	1.5e-107	65.92	Delta(7)-sterol-C5(6)-desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=STE1 PE=1 ...	[more]
Q9M883	1.8e-100	62.84	Putative Delta(7)-sterol-C5(6)-desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=H...	[more]
Q9EQS5	4.4e-27	33.76	Lathosterol oxidase OS=Rattus norvegicus OX=10116 GN=Sc5d PE=1 SV=1	[more]
O88822	4.9e-26	32.91	Lathosterol oxidase OS=Mus musculus OX=10090 GN=Sc5d PE=2 SV=2	[more]

BLAST of CsGy4G017510 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004150515.1	6.99e-216	100.00	delta(7)-sterol-C5(6)-desaturase [Cucumis sativus] >KGN54626.1 hypothetical prot...	[more]
XP_008444093.1	5.52e-213	99.27	PREDICTED: delta(7)-sterol-C5(6)-desaturase-like [Cucumis melo]	[more]
XP_038894105.1	2.23e-203	92.34	delta(7)-sterol-C5(6)-desaturase-like [Benincasa hispida] >XP_038894106.1 delta(...	[more]
XP_022980690.1	9.79e-198	90.15	delta(7)-sterol-C5(6)-desaturase-like isoform X1 [Cucurbita maxima]	[more]
XP_022956224.1	3.99e-197	89.42	delta(7)-sterol-C5(6)-desaturase-like [Cucurbita moschata] >KAG6581752.1 hypothe...	[more]

BLAST of CsGy4G017510 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L3B9	3.38e-216	100.00	Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=C...	[more]
A0A1S3BAC7	2.67e-213	99.27	delta(7)-sterol-C5(6)-desaturase-like OS=Cucumis melo OX=3656 GN=LOC103487532 PE...	[more]
A0A6J1IX84	4.74e-198	90.15	delta(7)-sterol-C5(6)-desaturase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A6J1GYF7	1.93e-197	89.42	delta(7)-sterol-C5(6)-desaturase-like OS=Cucurbita moschata OX=3662 GN=LOC111457...	[more]
A0A6J1KDR9	1.70e-191	87.59	delta(7)-sterol-C5(6)-desaturase-like OS=Cucurbita maxima OX=3661 GN=LOC11149293...	[more]

BLAST of CsGy4G017510 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G02580.1	1.1e-108	65.92	sterol 1	[more]
AT3G02590.1	1.3e-101	62.84	Fatty acid hydroxylase superfamily protein	[more]
AT1G69640.1	1.5e-06	27.27	sphingoid base hydroxylase 1	[more]
AT1G14290.1	1.3e-05	26.92	sphingoid base hydroxylase 2	[more]
AT1G02205.2	1.3e-05	29.80	Fatty acid hydroxylase superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006694	Fatty acid hydroxylase	PFAM	PF04116	FA_hydroxylase	coord: 133..262 e-value: 1.1E-20 score: 74.4
None	No IPR available	PANTHER	PTHR11863	STEROL DESATURASE	coord: 9..271
None	No IPR available	PANTHER	PTHR11863:SF160	DELTA(7)-STEROL-C5(6)-DESATURASE	coord: 9..271

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsGy4G017510.1	CsGy4G017510.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016126	sterol biosynthetic process
biological_process	GO:0008610	lipid biosynthetic process
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0000248	C-5 sterol desaturase activity
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016491	oxidoreductase activity