CsGy4G016600 (gene) Cucumber (Gy14) v2.1

Overview


Name	CsGy4G016600
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description	Calcium-binding EF-hand family protein
Location	Gy14Chr4: 21637825 .. 21638403 (-)
RNA-Seq Expression	CsGy4G016600
Synteny	CsGy4G016600

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGAAAATCCCCAAAAGATTTAGCCCTAAACGCCTCTTCCGATCCAAAAAGCATCACTCCGCCGACGTATCCCGCTCGGATCCACCCTCTTTCGGCTCCGGTACCTCCTCTTCCTCGTCCTCTTCCGAGAATTTCTTCAAAGCTAACACCGTGAACACCGGTTACGGAACCCCGACTAGCGTTCTGGACTCGGACGGGAGGGGGAACGAGGGGGTTGTGTCGAGGAAGGAACTGGAGGCGATTCTTAGCCGGATCGCGTCAGAGGAAGAGGTTGCGATGATGTTGCGCGAAGTGGATAGCGGGGGCGATGGGTTCATACGGTTGGAGGAATTGATGGCTCGGGTGGGGTCGGGTGAAGCGGCGGGAGAGTCGGAGATGCGGGAGACGTTTGAGTTTTTTGACGCGGATCAGGATGGGAGGATCACGGCGGAGGAGCTTCACGGGGTTTTTAGATCAATAGGAGATGAGCGGTGCACGTTAGAGGATTGCGGGCGTATGATAAGGGATGTGGATGAGAACGGGGATGGCTTTGTGTGTTTCGCGGAGTTTGTGCGTATGATGGAGCTGCAGAGATGA

mRNA sequence

ATGATGAAAATCCCCAAAAGATTTAGCCCTAAACGCCTCTTCCGATCCAAAAAGCATCACTCCGCCGACGTATCCCGCTCGGATCCACCCTCTTTCGGCTCCGGTACCTCCTCTTCCTCGTCCTCTTCCGAGAATTTCTTCAAAGCTAACACCGTGAACACCGGTTACGGAACCCCGACTAGCGTTCTGGACTCGGACGGGAGGGGGAACGAGGGGGTTGTGTCGAGGAAGGAACTGGAGGCGATTCTTAGCCGGATCGCGTCAGAGGAAGAGGTTGCGATGATGTTGCGCGAAGTGGATAGCGGGGGCGATGGGTTCATACGGTTGGAGGAATTGATGGCTCGGGTGGGGTCGGGTGAAGCGGCGGGAGAGTCGGAGATGCGGGAGACGTTTGAGTTTTTTGACGCGGATCAGGATGGGAGGATCACGGCGGAGGAGCTTCACGGGGTTTTTAGATCAATAGGAGATGAGCGGTGCACGTTAGAGGATTGCGGGCGTATGATAAGGGATGTGGATGAGAACGGGGATGGCTTTGTGTGTTTCGCGGAGTTTGTGCGTATGATGGAGCTGCAGAGATGA

Coding sequence (CDS)

ATGATGAAAATCCCCAAAAGATTTAGCCCTAAACGCCTCTTCCGATCCAAAAAGCATCACTCCGCCGACGTATCCCGCTCGGATCCACCCTCTTTCGGCTCCGGTACCTCCTCTTCCTCGTCCTCTTCCGAGAATTTCTTCAAAGCTAACACCGTGAACACCGGTTACGGAACCCCGACTAGCGTTCTGGACTCGGACGGGAGGGGGAACGAGGGGGTTGTGTCGAGGAAGGAACTGGAGGCGATTCTTAGCCGGATCGCGTCAGAGGAAGAGGTTGCGATGATGTTGCGCGAAGTGGATAGCGGGGGCGATGGGTTCATACGGTTGGAGGAATTGATGGCTCGGGTGGGGTCGGGTGAAGCGGCGGGAGAGTCGGAGATGCGGGAGACGTTTGAGTTTTTTGACGCGGATCAGGATGGGAGGATCACGGCGGAGGAGCTTCACGGGGTTTTTAGATCAATAGGAGATGAGCGGTGCACGTTAGAGGATTGCGGGCGTATGATAAGGGATGTGGATGAGAACGGGGATGGCTTTGTGTGTTTCGCGGAGTTTGTGCGTATGATGGAGCTGCAGAGATGA

Protein sequence

MMKIPKRFSPKRLFRSKKHHSADVSRSDPPSFGSGTSSSSSSSENFFKANTVNTGYGTPTSVLDSDGRGNEGVVSRKELEAILSRIASEEEVAMMLREVDSGGDGFIRLEELMARVGSGEAAGESEMRETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCGRMIRDVDENGDGFVCFAEFVRMMELQR*

Homology

[Close]

BLAST of CsGy4G016600 vs. ExPASy Swiss-Prot
Match: Q9SS31 (Probable calcium-binding protein CML36 OS=Arabidopsis thaliana OX=3702 GN=CML36 PE=2 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 2.7e-39
Identity = 108/212 (50.94%), Postives = 136/212 (64.15%), Query Frame = 0


Query: 2   MKIPKRFSPKRLFRSKKHHSADVSRSDPPSFGSGTSSSSSSSENFFKANTVNTGYGTPTS 61
           MK+ K   PKR F   K  S  VS+S P +F  G++SSSS  +          G  TPTS
Sbjct: 1   MKLAK-LIPKRFFIRSKDRST-VSKS-PTAFSFGSASSSSGQDCKNSGGDGGGGSVTPTS 60

Query: 62  VLDS----------------DGRGNEGVVSRKELEAILSRIA----SEEEVAMMLREVDS 121
           +L                    R N+G VSR +LE++LSR+     +EEE+ +ML+EVD 
Sbjct: 61  ILPEVPSPYSYVEILQAFKLIDRDNDGAVSRHDLESLLSRLGPDPLTEEEINVMLKEVDC 120

Query: 122 GGDGFIRLEELMARVGSGEAAGES-EMRETFEFFDADQDGRITAEELHGVFRSIGDERCT 181
            GDG IRLEEL +RV S + A +S E++ETFEFFDAD+DG I+A+EL  VF +IGDERCT
Sbjct: 121 DGDGTIRLEELASRVVSLDPARDSTELKETFEFFDADRDGLISADELLRVFSTIGDERCT 180

Query: 182 LEDCGRMIRDVDENGDGFVCFAEFVRMMELQR 193
           L+DC RMI DVDE+GDGFVCF EF RMM+LQR
Sbjct: 181 LDDCKRMIADVDEDGDGFVCFTEFSRMMDLQR 209

[Close]

BLAST of CsGy4G016600 vs. ExPASy Swiss-Prot
Match: P30188 (Probable calcium-binding protein CML35 OS=Arabidopsis thaliana OX=3702 GN=CML35 PE=2 SV=2)

HSP 1 Score: 159.5 bits (402), Expect = 3.9e-38
Identity = 105/204 (51.47%), Postives = 129/204 (63.24%), Query Frame = 0


Query: 7   RFSPKRLFRSKKHHSADVSRSDPPSFGSGTSSSSSSSENFFKANTVNTGYGTPTSVLDSD 66
           R SPKRLFR+K    A VSRS+P SF S  SSSSS        N       TP SVL  +
Sbjct: 9   RLSPKRLFRTKS--KASVSRSEPSSFSSNASSSSSDGS---YGNLKQGPTATPISVLPQN 68

Query: 67  G---------------RGNEGVVSRKELEAILSRIA----SEEEVAMMLREVDSGGDGFI 126
                           R ++GVVSR +L A++SR++    S+EEV++MLREVD G  G I
Sbjct: 69  SGDFYTELVQAFKLIDRDDDGVVSRGDLAALISRLSHEPPSQEEVSLMLREVDGGDGGCI 128

Query: 127 RLEELMARVG--SGEAAGES-EMRETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDC 186
            LE+L +RV   SGE + E+ E+RE FE FD D++G+I+AEELH VF  IGDERCTLE+C
Sbjct: 129 SLEDLASRVAGTSGEGSVETEELREVFEIFDVDRNGKISAEELHRVFGVIGDERCTLEEC 188

Query: 187 GRMIRDVDENGDGFVCFAEFVRMM 189
            RMI  VD NGDGFVCF +F RMM
Sbjct: 189 MRMIATVDGNGDGFVCFDDFCRMM 207

[Close]

BLAST of CsGy4G016600 vs. ExPASy Swiss-Prot
Match: Q0DJV6 (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 GN=CML18 PE=2 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 2.4e-19
Identity = 58/135 (42.96%), Postives = 80/135 (59.26%), Query Frame = 0


Query: 71  EGVVSRKELEAILSRIAS---EEEVAMMLREVDSGGDGFIRLEELM--------ARVGSG 130
           +G +S +EL ++L  + +     EV  M+ E+DS  DGF+ L E          A  G G
Sbjct: 18  DGKISAEELASVLRALGAPLGPGEVRRMMDEMDSDRDGFVDLSEFAAFHCGPTPAHGGKG 77

Query: 131 ------EAAGESEMRETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCGRMIRDVDE 189
                 EAA E+E+RE F  +DAD +G+I+A ELH V R +GD +C++ DC RMIR VD 
Sbjct: 78  GDAKDQEAASEAELREAFRMYDADSNGKISARELHRVLRQLGD-KCSVADCSRMIRSVDA 137

[Close]

BLAST of CsGy4G016600 vs. ExPASy Swiss-Prot
Match: Q9C8Y1 (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 PE=2 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 2.4e-19
Identity = 52/128 (40.62%), Postives = 83/128 (64.84%), Query Frame = 0


Query: 68  RGNEGVVSRKELEAI---LSRIASEEEVAMMLREVDSGGDGFIRLEELMARVGSGEAAGE 127
           + N+G +S  EL+ +   LS  AS+EE   M++E D  G+GFI L+E +A     + +  
Sbjct: 25  KNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFVALFQISDQSSN 84

Query: 128 S----EMRETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCGRMIRDVDENGDGFVC 187
           +    +++E F+ +D D++GRI+A ELH V +++G E+C+++DC RMI  VD +GDG V 
Sbjct: 85  NSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLG-EKCSIQDCQRMINKVDSDGDGCVD 144

Query: 188 FAEFVRMM 189
           F EF +MM
Sbjct: 145 FEEFKKMM 151

[Close]

BLAST of CsGy4G016600 vs. ExPASy Swiss-Prot
Match: P25070 (Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2)

HSP 1 Score: 97.1 bits (240), Expect = 2.4e-19
Identity = 55/131 (41.98%), Postives = 82/131 (62.60%), Query Frame = 0


Query: 64  DSDGRGNEGVVSRKELEAILSRIASEEEVAMMLREVDSGGDGFIRLEELMA--RVGSGEA 123
           D +G G   V   KE+   LS  AS EE   M+++ D  G+GFI L+E +A  ++G G  
Sbjct: 26  DKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVALFQIGIGGG 85

Query: 124 AGE----SEMRETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCGRMIRDVDENGDG 183
                  S+++E FE +D D +GRI+A+ELH V +++G E+C+++DC +MI  VD +GDG
Sbjct: 86  GNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLG-EKCSVQDCKKMISKVDIDGDG 145

Query: 184 FVCFAEFVRMM 189
            V F EF +MM
Sbjct: 146 CVNFDEFKKMM 155

[Close]

BLAST of CsGy4G016600 vs. NCBI nr
Match: XP_004151276.1 (probable calcium-binding protein CML36 [Cucumis sativus] >KGN54524.1 hypothetical protein Csa_012346 [Cucumis sativus])

HSP 1 Score: 371 bits (952), Expect = 2.83e-129
Identity = 192/192 (100.00%), Postives = 192/192 (100.00%), Query Frame = 0


Query: 1   MMKIPKRFSPKRLFRSKKHHSADVSRSDPPSFGSGTSSSSSSSENFFKANTVNTGYGTPT 60
           MMKIPKRFSPKRLFRSKKHHSADVSRSDPPSFGSGTSSSSSSSENFFKANTVNTGYGTPT
Sbjct: 1   MMKIPKRFSPKRLFRSKKHHSADVSRSDPPSFGSGTSSSSSSSENFFKANTVNTGYGTPT 60

Query: 61  SVLDSDGRGNEGVVSRKELEAILSRIASEEEVAMMLREVDSGGDGFIRLEELMARVGSGE 120
           SVLDSDGRGNEGVVSRKELEAILSRIASEEEVAMMLREVDSGGDGFIRLEELMARVGSGE
Sbjct: 61  SVLDSDGRGNEGVVSRKELEAILSRIASEEEVAMMLREVDSGGDGFIRLEELMARVGSGE 120

Query: 121 AAGESEMRETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCGRMIRDVDENGDGFVC 180
           AAGESEMRETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCGRMIRDVDENGDGFVC
Sbjct: 121 AAGESEMRETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCGRMIRDVDENGDGFVC 180

Query: 181 FAEFVRMMELQR 192
           FAEFVRMMELQR
Sbjct: 181 FAEFVRMMELQR 192

[Close]

BLAST of CsGy4G016600 vs. NCBI nr
Match: XP_008457589.1 (PREDICTED: probable calcium-binding protein CML36 [Cucumis melo] >KAA0062715.1 putative calcium-binding protein CML36 [Cucumis melo var. makuwa])

HSP 1 Score: 348 bits (894), Expect = 1.97e-120
Identity = 182/192 (94.79%), Postives = 186/192 (96.88%), Query Frame = 0


Query: 1   MMKIPKRFSPKRLFRSKKHHSADVSRSDPPSFGSGTSSSSSSSENFFKANTVNTGYGTPT 60
           MMKIPKRFSPKRLFRSKKH SADVSRSDPPSFGSGTSSSSSSSENFFK N+VNTGYGTPT
Sbjct: 1   MMKIPKRFSPKRLFRSKKHQSADVSRSDPPSFGSGTSSSSSSSENFFKPNSVNTGYGTPT 60

Query: 61  SVLDSDGRGNEGVVSRKELEAILSRIASEEEVAMMLREVDSGGDGFIRLEELMARVGSGE 120
           SVLDSD R NEGVV+RKELEAILSRIASEEEVAMML EVDSGGDGFIRLEELMARVGSGE
Sbjct: 61  SVLDSDVRENEGVVTRKELEAILSRIASEEEVAMMLSEVDSGGDGFIRLEELMARVGSGE 120

Query: 121 AAGESEMRETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCGRMIRDVDENGDGFVC 180
           AAGESE+RETFEFFDADQDGRITAEELHGVFRSIGDE+CTLEDC RMIRDVDENGDGFVC
Sbjct: 121 AAGESELRETFEFFDADQDGRITAEELHGVFRSIGDEQCTLEDCRRMIRDVDENGDGFVC 180

Query: 181 FAEFVRMMELQR 192
           FAEFVRMMELQR
Sbjct: 181 FAEFVRMMELQR 192

[Close]

BLAST of CsGy4G016600 vs. NCBI nr
Match: XP_038883626.1 (probable calcium-binding protein CML35 [Benincasa hispida])

HSP 1 Score: 306 bits (783), Expect = 2.14e-103
Identity = 170/199 (85.43%), Postives = 176/199 (88.44%), Query Frame = 0


Query: 2   MKIPKRFSPKRLFRSKKHHSADVSRSDPPSFGSGTSSSSSSSENFFKANTVNTGYGTPTS 61
           MKIPKRFSPKRLFRSKK+HSADVSRSDPPSFGSGTSSSSSSSE FFK N   TGYGTPTS
Sbjct: 1   MKIPKRFSPKRLFRSKKYHSADVSRSDPPSFGSGTSSSSSSSETFFKPNATATGYGTPTS 60

Query: 62  VL-----DSDGRGNEGVVSRKELEAILSRIASEEEVAMMLREVDSGG---DGFIRLEELM 121
           VL     DS  R N+GVVSRKELEAILSRIASEEEVAMML EV SGG   DGFI LEELM
Sbjct: 61  VLPPDWPDSGQRENDGVVSRKELEAILSRIASEEEVAMMLSEVSSGGGGGDGFIWLEELM 120

Query: 122 ARVGSG-EAAGESEMRETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCGRMIRDVD 181
           A+VG+G EAAGESE+RETFEFFDADQDGRITAEELH VFRSIGDE+CTLEDC RMIRDVD
Sbjct: 121 AQVGTGVEAAGESELRETFEFFDADQDGRITAEELHEVFRSIGDEQCTLEDCRRMIRDVD 180

Query: 182 ENGDGFVCFAEFVRMMELQ 191
           ENGDG VCFAEFVRMMELQ
Sbjct: 181 ENGDGVVCFAEFVRMMELQ 199

[Close]

BLAST of CsGy4G016600 vs. NCBI nr
Match: XP_022932888.1 (probable calcium-binding protein CML36 [Cucurbita moschata] >KAG6603330.1 putative calcium-binding protein CML36, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 300 bits (767), Expect = 4.60e-101
Identity = 166/197 (84.26%), Postives = 176/197 (89.34%), Query Frame = 0


Query: 2   MKIPKRFSPKRLFRSKKHHSADVSRSDPPSFGSGTSSSSSSSENFFKANTVNTGYGTPTS 61
           MKIPKRFSPKRLFRSKK+ SA+VSRSDPPSFGSGTSSSSSSSENFFK +T   GYGTPTS
Sbjct: 1   MKIPKRFSPKRLFRSKKNPSAEVSRSDPPSFGSGTSSSSSSSENFFKPHTA--GYGTPTS 60

Query: 62  VL-----DSDGRGNEGVVSRKELEAILSRIASEEEVAMMLREVDSGGDGFIRLEELMARV 121
           VL     DSD R N+GVVSRKELEAILSRIASEEEVAMML EVD GG  FIRL+ELMARV
Sbjct: 61  VLLAGCSDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGGG--FIRLDELMARV 120

Query: 122 GSG-EAAGESEMRETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCGRMIRDVDENG 181
           GSG +A GESE+RETFEFFDAD DGRI+AEELHGV+RSIGDERCTLEDC RMIRDVDE+G
Sbjct: 121 GSGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDEDG 180

Query: 182 DGFVCFAEFVRMMELQR 192
           DGFVCF EFVRMMELQR
Sbjct: 181 DGFVCFTEFVRMMELQR 193

[Close]

BLAST of CsGy4G016600 vs. NCBI nr
Match: XP_022967907.1 (probable calcium-binding protein CML36 [Cucurbita maxima])

HSP 1 Score: 298 bits (763), Expect = 1.81e-100
Identity = 166/196 (84.69%), Postives = 175/196 (89.29%), Query Frame = 0


Query: 2   MKIPKRFSPKRLFRSKKHHSADVSRSDPPSFGSGTSSSSSSSENFFKANTVNTGYGTPTS 61
           MKIPKRFSPKRLFRSKK+ SADVSRSDPPSFGSGTSSSSSSSENFFK +T   GYGTPTS
Sbjct: 1   MKIPKRFSPKRLFRSKKNPSADVSRSDPPSFGSGTSSSSSSSENFFKPHTA--GYGTPTS 60

Query: 62  VL----DSDGRGNEGVVSRKELEAILSRIASEEEVAMMLREVDSGGDGFIRLEELMARVG 121
           VL    DSD R N+ VVSRKELEAILSRIASEEEVAMML EVD GG  FIRL+ELMARVG
Sbjct: 61  VLAGCSDSDQRENDVVVSRKELEAILSRIASEEEVAMMLSEVDDGG--FIRLDELMARVG 120

Query: 122 SG-EAAGESEMRETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCGRMIRDVDENGD 181
           SG +A GESE+RETFEFFDAD DGRI+AEELHGV+RSIGDERCTLEDC RMIRDVDE+GD
Sbjct: 121 SGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDEDGD 180

Query: 182 GFVCFAEFVRMMELQR 192
           GFVCF EFVRMMELQR
Sbjct: 181 GFVCFTEFVRMMELQR 192

[Close]

BLAST of CsGy4G016600 vs. ExPASy TrEMBL
Match: A0A0A0L0H1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G355130 PE=4 SV=1)

HSP 1 Score: 371 bits (952), Expect = 1.37e-129
Identity = 192/192 (100.00%), Postives = 192/192 (100.00%), Query Frame = 0


Query: 1   MMKIPKRFSPKRLFRSKKHHSADVSRSDPPSFGSGTSSSSSSSENFFKANTVNTGYGTPT 60
           MMKIPKRFSPKRLFRSKKHHSADVSRSDPPSFGSGTSSSSSSSENFFKANTVNTGYGTPT
Sbjct: 1   MMKIPKRFSPKRLFRSKKHHSADVSRSDPPSFGSGTSSSSSSSENFFKANTVNTGYGTPT 60

Query: 61  SVLDSDGRGNEGVVSRKELEAILSRIASEEEVAMMLREVDSGGDGFIRLEELMARVGSGE 120
           SVLDSDGRGNEGVVSRKELEAILSRIASEEEVAMMLREVDSGGDGFIRLEELMARVGSGE
Sbjct: 61  SVLDSDGRGNEGVVSRKELEAILSRIASEEEVAMMLREVDSGGDGFIRLEELMARVGSGE 120

Query: 121 AAGESEMRETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCGRMIRDVDENGDGFVC 180
           AAGESEMRETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCGRMIRDVDENGDGFVC
Sbjct: 121 AAGESEMRETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCGRMIRDVDENGDGFVC 180

Query: 181 FAEFVRMMELQR 192
           FAEFVRMMELQR
Sbjct: 181 FAEFVRMMELQR 192

[Close]

BLAST of CsGy4G016600 vs. ExPASy TrEMBL
Match: A0A5A7VA05 (Putative calcium-binding protein CML36 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold382G00410 PE=4 SV=1)

HSP 1 Score: 348 bits (894), Expect = 9.55e-121
Identity = 182/192 (94.79%), Postives = 186/192 (96.88%), Query Frame = 0


Query: 1   MMKIPKRFSPKRLFRSKKHHSADVSRSDPPSFGSGTSSSSSSSENFFKANTVNTGYGTPT 60
           MMKIPKRFSPKRLFRSKKH SADVSRSDPPSFGSGTSSSSSSSENFFK N+VNTGYGTPT
Sbjct: 1   MMKIPKRFSPKRLFRSKKHQSADVSRSDPPSFGSGTSSSSSSSENFFKPNSVNTGYGTPT 60

Query: 61  SVLDSDGRGNEGVVSRKELEAILSRIASEEEVAMMLREVDSGGDGFIRLEELMARVGSGE 120
           SVLDSD R NEGVV+RKELEAILSRIASEEEVAMML EVDSGGDGFIRLEELMARVGSGE
Sbjct: 61  SVLDSDVRENEGVVTRKELEAILSRIASEEEVAMMLSEVDSGGDGFIRLEELMARVGSGE 120

Query: 121 AAGESEMRETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCGRMIRDVDENGDGFVC 180
           AAGESE+RETFEFFDADQDGRITAEELHGVFRSIGDE+CTLEDC RMIRDVDENGDGFVC
Sbjct: 121 AAGESELRETFEFFDADQDGRITAEELHGVFRSIGDEQCTLEDCRRMIRDVDENGDGFVC 180

Query: 181 FAEFVRMMELQR 192
           FAEFVRMMELQR
Sbjct: 181 FAEFVRMMELQR 192

[Close]

BLAST of CsGy4G016600 vs. ExPASy TrEMBL
Match: A0A1S3C5U3 (probable calcium-binding protein CML36 OS=Cucumis melo OX=3656 GN=LOC103497255 PE=4 SV=1)

HSP 1 Score: 348 bits (894), Expect = 9.55e-121
Identity = 182/192 (94.79%), Postives = 186/192 (96.88%), Query Frame = 0


Query: 1   MMKIPKRFSPKRLFRSKKHHSADVSRSDPPSFGSGTSSSSSSSENFFKANTVNTGYGTPT 60
           MMKIPKRFSPKRLFRSKKH SADVSRSDPPSFGSGTSSSSSSSENFFK N+VNTGYGTPT
Sbjct: 1   MMKIPKRFSPKRLFRSKKHQSADVSRSDPPSFGSGTSSSSSSSENFFKPNSVNTGYGTPT 60

Query: 61  SVLDSDGRGNEGVVSRKELEAILSRIASEEEVAMMLREVDSGGDGFIRLEELMARVGSGE 120
           SVLDSD R NEGVV+RKELEAILSRIASEEEVAMML EVDSGGDGFIRLEELMARVGSGE
Sbjct: 61  SVLDSDVRENEGVVTRKELEAILSRIASEEEVAMMLSEVDSGGDGFIRLEELMARVGSGE 120

Query: 121 AAGESEMRETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCGRMIRDVDENGDGFVC 180
           AAGESE+RETFEFFDADQDGRITAEELHGVFRSIGDE+CTLEDC RMIRDVDENGDGFVC
Sbjct: 121 AAGESELRETFEFFDADQDGRITAEELHGVFRSIGDEQCTLEDCRRMIRDVDENGDGFVC 180

Query: 181 FAEFVRMMELQR 192
           FAEFVRMMELQR
Sbjct: 181 FAEFVRMMELQR 192

[Close]

BLAST of CsGy4G016600 vs. ExPASy TrEMBL
Match: A0A6J1F310 (probable calcium-binding protein CML36 OS=Cucurbita moschata OX=3662 GN=LOC111439413 PE=4 SV=1)

HSP 1 Score: 300 bits (767), Expect = 2.23e-101
Identity = 166/197 (84.26%), Postives = 176/197 (89.34%), Query Frame = 0


Query: 2   MKIPKRFSPKRLFRSKKHHSADVSRSDPPSFGSGTSSSSSSSENFFKANTVNTGYGTPTS 61
           MKIPKRFSPKRLFRSKK+ SA+VSRSDPPSFGSGTSSSSSSSENFFK +T   GYGTPTS
Sbjct: 1   MKIPKRFSPKRLFRSKKNPSAEVSRSDPPSFGSGTSSSSSSSENFFKPHTA--GYGTPTS 60

Query: 62  VL-----DSDGRGNEGVVSRKELEAILSRIASEEEVAMMLREVDSGGDGFIRLEELMARV 121
           VL     DSD R N+GVVSRKELEAILSRIASEEEVAMML EVD GG  FIRL+ELMARV
Sbjct: 61  VLLAGCSDSDQRENDGVVSRKELEAILSRIASEEEVAMMLSEVDGGG--FIRLDELMARV 120

Query: 122 GSG-EAAGESEMRETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCGRMIRDVDENG 181
           GSG +A GESE+RETFEFFDAD DGRI+AEELHGV+RSIGDERCTLEDC RMIRDVDE+G
Sbjct: 121 GSGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDEDG 180

Query: 182 DGFVCFAEFVRMMELQR 192
           DGFVCF EFVRMMELQR
Sbjct: 181 DGFVCFTEFVRMMELQR 193

[Close]

BLAST of CsGy4G016600 vs. ExPASy TrEMBL
Match: A0A6J1HS37 (probable calcium-binding protein CML36 OS=Cucurbita maxima OX=3661 GN=LOC111467281 PE=4 SV=1)

HSP 1 Score: 298 bits (763), Expect = 8.76e-101
Identity = 166/196 (84.69%), Postives = 175/196 (89.29%), Query Frame = 0


Query: 2   MKIPKRFSPKRLFRSKKHHSADVSRSDPPSFGSGTSSSSSSSENFFKANTVNTGYGTPTS 61
           MKIPKRFSPKRLFRSKK+ SADVSRSDPPSFGSGTSSSSSSSENFFK +T   GYGTPTS
Sbjct: 1   MKIPKRFSPKRLFRSKKNPSADVSRSDPPSFGSGTSSSSSSSENFFKPHTA--GYGTPTS 60

Query: 62  VL----DSDGRGNEGVVSRKELEAILSRIASEEEVAMMLREVDSGGDGFIRLEELMARVG 121
           VL    DSD R N+ VVSRKELEAILSRIASEEEVAMML EVD GG  FIRL+ELMARVG
Sbjct: 61  VLAGCSDSDQRENDVVVSRKELEAILSRIASEEEVAMMLSEVDDGG--FIRLDELMARVG 120

Query: 122 SG-EAAGESEMRETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCGRMIRDVDENGD 181
           SG +A GESE+RETFEFFDAD DGRI+AEELHGV+RSIGDERCTLEDC RMIRDVDE+GD
Sbjct: 121 SGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDEDGD 180

Query: 182 GFVCFAEFVRMMELQR 192
           GFVCF EFVRMMELQR
Sbjct: 181 GFVCFTEFVRMMELQR 192

[Close]

BLAST of CsGy4G016600 vs. TAIR 10
Match: AT3G10190.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 163.3 bits (412), Expect = 1.9e-40
Identity = 108/212 (50.94%), Postives = 136/212 (64.15%), Query Frame = 0


Query: 2   MKIPKRFSPKRLFRSKKHHSADVSRSDPPSFGSGTSSSSSSSENFFKANTVNTGYGTPTS 61
           MK+ K   PKR F   K  S  VS+S P +F  G++SSSS  +          G  TPTS
Sbjct: 1   MKLAK-LIPKRFFIRSKDRST-VSKS-PTAFSFGSASSSSGQDCKNSGGDGGGGSVTPTS 60

Query: 62  VLDS----------------DGRGNEGVVSRKELEAILSRIA----SEEEVAMMLREVDS 121
           +L                    R N+G VSR +LE++LSR+     +EEE+ +ML+EVD 
Sbjct: 61  ILPEVPSPYSYVEILQAFKLIDRDNDGAVSRHDLESLLSRLGPDPLTEEEINVMLKEVDC 120

Query: 122 GGDGFIRLEELMARVGSGEAAGES-EMRETFEFFDADQDGRITAEELHGVFRSIGDERCT 181
            GDG IRLEEL +RV S + A +S E++ETFEFFDAD+DG I+A+EL  VF +IGDERCT
Sbjct: 121 DGDGTIRLEELASRVVSLDPARDSTELKETFEFFDADRDGLISADELLRVFSTIGDERCT 180

Query: 182 LEDCGRMIRDVDENGDGFVCFAEFVRMMELQR 193
           L+DC RMI DVDE+GDGFVCF EF RMM+LQR
Sbjct: 181 LDDCKRMIADVDEDGDGFVCFTEFSRMMDLQR 209

[Close]

BLAST of CsGy4G016600 vs. TAIR 10
Match: AT2G41410.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 159.5 bits (402), Expect = 2.8e-39
Identity = 105/204 (51.47%), Postives = 129/204 (63.24%), Query Frame = 0


Query: 7   RFSPKRLFRSKKHHSADVSRSDPPSFGSGTSSSSSSSENFFKANTVNTGYGTPTSVLDSD 66
           R SPKRLFR+K    A VSRS+P SF S  SSSSS        N       TP SVL  +
Sbjct: 9   RLSPKRLFRTKS--KASVSRSEPSSFSSNASSSSSDGS---YGNLKQGPTATPISVLPQN 68

Query: 67  G---------------RGNEGVVSRKELEAILSRIA----SEEEVAMMLREVDSGGDGFI 126
                           R ++GVVSR +L A++SR++    S+EEV++MLREVD G  G I
Sbjct: 69  SGDFYTELVQAFKLIDRDDDGVVSRGDLAALISRLSHEPPSQEEVSLMLREVDGGDGGCI 128

Query: 127 RLEELMARVG--SGEAAGES-EMRETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDC 186
            LE+L +RV   SGE + E+ E+RE FE FD D++G+I+AEELH VF  IGDERCTLE+C
Sbjct: 129 SLEDLASRVAGTSGEGSVETEELREVFEIFDVDRNGKISAEELHRVFGVIGDERCTLEEC 188

Query: 187 GRMIRDVDENGDGFVCFAEFVRMM 189
            RMI  VD NGDGFVCF +F RMM
Sbjct: 189 MRMIATVDGNGDGFVCFDDFCRMM 207

[Close]

BLAST of CsGy4G016600 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 97.1 bits (240), Expect = 1.7e-20
Identity = 52/128 (40.62%), Postives = 83/128 (64.84%), Query Frame = 0


Query: 68  RGNEGVVSRKELEAI---LSRIASEEEVAMMLREVDSGGDGFIRLEELMARVGSGEAAGE 127
           + N+G +S  EL+ +   LS  AS+EE   M++E D  G+GFI L+E +A     + +  
Sbjct: 25  KNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFVALFQISDQSSN 84

Query: 128 S----EMRETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCGRMIRDVDENGDGFVC 187
           +    +++E F+ +D D++GRI+A ELH V +++G E+C+++DC RMI  VD +GDG V 
Sbjct: 85  NSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLG-EKCSIQDCQRMINKVDSDGDGCVD 144

Query: 188 FAEFVRMM 189
           F EF +MM
Sbjct: 145 FEEFKKMM 151

[Close]

BLAST of CsGy4G016600 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 97.1 bits (240), Expect = 1.7e-20
Identity = 55/131 (41.98%), Postives = 82/131 (62.60%), Query Frame = 0


Query: 64  DSDGRGNEGVVSRKELEAILSRIASEEEVAMMLREVDSGGDGFIRLEELMA--RVGSGEA 123
           D +G G   V   KE+   LS  AS EE   M+++ D  G+GFI L+E +A  ++G G  
Sbjct: 26  DKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVALFQIGIGGG 85

Query: 124 AGE----SEMRETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCGRMIRDVDENGDG 183
                  S+++E FE +D D +GRI+A+ELH V +++G E+C+++DC +MI  VD +GDG
Sbjct: 86  GNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLG-EKCSVQDCKKMISKVDIDGDG 145

Query: 184 FVCFAEFVRMM 189
            V F EF +MM
Sbjct: 146 CVNFDEFKKMM 155

[Close]

BLAST of CsGy4G016600 vs. TAIR 10
Match: AT2G15680.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 89.0 bits (219), Expect = 4.6e-18
Identity = 51/129 (39.53%), Postives = 74/129 (57.36%), Query Frame = 0


Query: 63  LDSDGRGNEGVVSRKELEAILSRIASE---EEVAMMLREVDSGGDGFIRLEELMARVGSG 122
           LD DG+     +S+ E + +L  +  E   E+V  + + VD  GDGFI   E +      
Sbjct: 60  LDKDGK-----ISQTEYKVVLRALGQERAIEDVPKIFKAVDLDGDGFIDFREFIDAYKRS 119

Query: 123 EAAGESEMRETFEFFDADQDGRITAEELHGVFRSIGDERCTLEDCGRMIRDVDENGDGFV 182
                S++R +F  FD + DG+I+AEE+  V   +G ERC+LEDC RM+R VD +GDG V
Sbjct: 120 GGIRSSDIRNSFWTFDLNGDGKISAEEVMSVLWKLG-ERCSLEDCNRMVRAVDADGDGLV 179

Query: 183 CFAEFVRMM 189
              EF++MM
Sbjct: 180 NMEEFIKMM 182

The following BLAST results are available for this feature:

BLAST of CsGy4G016600 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SS31	2.7e-39	50.94	Probable calcium-binding protein CML36 OS=Arabidopsis thaliana OX=3702 GN=CML36 ...	[more]
P30188	3.9e-38	51.47	Probable calcium-binding protein CML35 OS=Arabidopsis thaliana OX=3702 GN=CML35 ...	[more]
Q0DJV6	2.4e-19	42.96	Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9C8Y1	2.4e-19	40.63	Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 ...	[more]
P25070	2.4e-19	41.98	Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2	[more]

BLAST of CsGy4G016600 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004151276.1	2.83e-129	100.00	probable calcium-binding protein CML36 [Cucumis sativus] >KGN54524.1 hypothetica...	[more]
XP_008457589.1	1.97e-120	94.79	PREDICTED: probable calcium-binding protein CML36 [Cucumis melo] >KAA0062715.1 p...	[more]
XP_038883626.1	2.14e-103	85.43	probable calcium-binding protein CML35 [Benincasa hispida]	[more]
XP_022932888.1	4.60e-101	84.26	probable calcium-binding protein CML36 [Cucurbita moschata] >KAG6603330.1 putati...	[more]
XP_022967907.1	1.81e-100	84.69	probable calcium-binding protein CML36 [Cucurbita maxima]	[more]

BLAST of CsGy4G016600 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L0H1	1.37e-129	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G355130 PE=4 SV=1	[more]
A0A5A7VA05	9.55e-121	94.79	Putative calcium-binding protein CML36 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3C5U3	9.55e-121	94.79	probable calcium-binding protein CML36 OS=Cucumis melo OX=3656 GN=LOC103497255 P...	[more]
A0A6J1F310	2.23e-101	84.26	probable calcium-binding protein CML36 OS=Cucurbita moschata OX=3662 GN=LOC11143...	[more]
A0A6J1HS37	8.76e-101	84.69	probable calcium-binding protein CML36 OS=Cucurbita maxima OX=3661 GN=LOC1114672...	[more]

BLAST of CsGy4G016600 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G10190.1	1.9e-40	50.94	Calcium-binding EF-hand family protein	[more]
AT2G41410.1	2.8e-39	51.47	Calcium-binding EF-hand family protein	[more]
AT1G66400.1	1.7e-20	40.63	calmodulin like 23	[more]
AT5G37770.1	1.7e-20	41.98	EF hand calcium-binding protein family	[more]
AT2G15680.1	4.6e-18	39.53	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 126..154 e-value: 1.3E-4 score: 31.3 coord: 91..119 e-value: 1.4 score: 16.6 coord: 163..191 e-value: 1.5E-4 score: 31.1
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 125..188 e-value: 4.9E-9 score: 36.5
IPR002048	EF-hand domain	PFAM	PF13833	EF-hand_8	coord: 72..115 e-value: 5.6E-5 score: 22.8
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 122..157 score: 14.290241
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 159..192 score: 12.281773
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 126..188 e-value: 5.02027E-17 score: 69.8841
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 91..147 e-value: 6.17476E-7 score: 42.9201
None	No IPR available	GENE3D	1.10.238.10		coord: 60..114 e-value: 3.8E-8 score: 35.4
None	No IPR available	GENE3D	1.10.238.10		coord: 115..192 e-value: 1.8E-19 score: 72.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..19
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..44
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 27..44
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 1..190
None	No IPR available	PANTHER	PTHR10891:SF790	CALCIUM-BINDING PROTEIN CML35-RELATED	coord: 1..190
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 135..147
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 172..184
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 70..188

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsGy4G016600.1	CsGy4G016600.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding