CsGy4G010005 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy4G010005
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionAcetate kinase
LocationGy14Chr4: 8470950 .. 8471510 (-)
RNA-Seq ExpressionCsGy4G010005
SyntenyCsGy4G010005
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGTGCAACTACCGCTAGAATAAAGGCAGCAACAATCATATAGCTTTGCTTAATTAATTTCTACCCAACCAATTAAAAATTAGGATGAGTGATTTTGTTGAAATGGTAGTCCCTAATAATCAAGAAGATTGCAAACAAATTTCAAATGTCGAGTATAATTCGTATTTGCAAGCAATGGGAAGGTTTGCAGTGGAGGAGTATACACATTACAATAAAGGGATAAAAAGAAAGTTCATCAGTGTAGGGTTTGCCGTTGTTTGCTCAGTTTCTGTGGGAGAAGAAAAATATCATCTCTTAGTGAAGGTAGATGAATTTATATTAGCTCCATCTGTCCCAAAATTTGAAAAGGTATATGTGGCTACTGTGCTTCATAAAAAAGTTAACCACTCGTGGGCACTCTTACGATTTGAAGATGCGGCAGCTTCTACTGTTTCCCCCACGAACATTGAATATCCTGTGTATGCATAGCTTAGTTGTCTACTTTAGATGATGATGCACATGTTGCTTTTATCTTTATATCTATCAAATATTTTGGAAGTAATCAAAATAAAGGATGTTGTAT

mRNA sequence

TGTGCAACTACCGCTAGAATAAAGGCAGCAACAATCATATAGCTTTGCTTAATTAATTTCTACCCAACCAATTAAAAATTAGGATGAGTGATTTTGTTGAAATGGTAGTCCCTAATAATCAAGAAGATTGCAAACAAATTTCAAATGTCGAGTATAATTCGTATTTGCAAGCAATGGGAAGGTTTGCAGTGGAGGAGTATACACATTACAATAAAGGGATAAAAAGAAAGTTCATCAGTGTAGGGTTTGCCGTTGTTTGCTCAGTTTCTGTGGGAGAAGAAAAATATCATCTCTTAGTGAAGGTAGATGAATTTATATTAGCTCCATCTGTCCCAAAATTTGAAAAGGTATATGTGGCTACTGTGCTTCATAAAAAAGTTAACCACTCGTGGGCACTCTTACGATTTGAAGATGCGGCAGCTTCTACTGTTTCCCCCACGAACATTGAATATCCTGTGTATGCATAGCTTAGTTGTCTACTTTAGATGATGATGCACATGTTGCTTTTATCTTTATATCTATCAAATATTTTGGAAGTAATCAAAATAAAGGATGTTGTAT

Coding sequence (CDS)

ATGAGTGATTTTGTTGAAATGGTAGTCCCTAATAATCAAGAAGATTGCAAACAAATTTCAAATGTCGAGTATAATTCGTATTTGCAAGCAATGGGAAGGTTTGCAGTGGAGGAGTATACACATTACAATAAAGGGATAAAAAGAAAGTTCATCAGTGTAGGGTTTGCCGTTGTTTGCTCAGTTTCTGTGGGAGAAGAAAAATATCATCTCTTAGTGAAGGTAGATGAATTTATATTAGCTCCATCTGTCCCAAAATTTGAAAAGGTATATGTGGCTACTGTGCTTCATAAAAAAGTTAACCACTCGTGGGCACTCTTACGATTTGAAGATGCGGCAGCTTCTACTGTTTCCCCCACGAACATTGAATATCCTGTGTATGCATAG

Protein sequence

MSDFVEMVVPNNQEDCKQISNVEYNSYLQAMGRFAVEEYTHYNKGIKRKFISVGFAVVCSVSVGEEKYHLLVKVDEFILAPSVPKFEKVYVATVLHKKVNHSWALLRFEDAAASTVSPTNIEYPVYA*
Homology
BLAST of CsGy4G010005 vs. NCBI nr
Match: KAE8649343.1 (hypothetical protein Csa_014384 [Cucumis sativus])

HSP 1 Score: 256 bits (654), Expect = 5.86e-86
Identity = 127/127 (100.00%), Postives = 127/127 (100.00%), Query Frame = 0

Query: 1   MSDFVEMVVPNNQEDCKQISNVEYNSYLQAMGRFAVEEYTHYNKGIKRKFISVGFAVVCS 60
           MSDFVEMVVPNNQEDCKQISNVEYNSYLQAMGRFAVEEYTHYNKGIKRKFISVGFAVVCS
Sbjct: 1   MSDFVEMVVPNNQEDCKQISNVEYNSYLQAMGRFAVEEYTHYNKGIKRKFISVGFAVVCS 60

Query: 61  VSVGEEKYHLLVKVDEFILAPSVPKFEKVYVATVLHKKVNHSWALLRFEDAAASTVSPTN 120
           VSVGEEKYHLLVKVDEFILAPSVPKFEKVYVATVLHKKVNHSWALLRFEDAAASTVSPTN
Sbjct: 61  VSVGEEKYHLLVKVDEFILAPSVPKFEKVYVATVLHKKVNHSWALLRFEDAAASTVSPTN 120

Query: 121 IEYPVYA 127
           IEYPVYA
Sbjct: 121 IEYPVYA 127

BLAST of CsGy4G010005 vs. NCBI nr
Match: KGN53820.1 (hypothetical protein Csa_014847 [Cucumis sativus])

HSP 1 Score: 189 bits (480), Expect = 1.99e-59
Identity = 92/119 (77.31%), Postives = 104/119 (87.39%), Query Frame = 0

Query: 5   VEMVVPNNQEDCKQISNVEYNSYLQAMGRFAVEEYTHYNKGIKRKFISVGFAVVCSVSVG 64
           V  VVPN++EDCKQISNVEYN YLQAMG+FAVEE+TH   GI+RKFISVGFAVVCSV VG
Sbjct: 6   VATVVPNDREDCKQISNVEYNEYLQAMGKFAVEEHTHNMSGIERKFISVGFAVVCSVPVG 65

Query: 65  EEKYHLLVKVDEFILAPSVPKFEKVYVATVLHKKVNHSWALLRFEDAAASTVSPTNIEY 123
            +KYHLLVKVDEFILAPS PK+EK+Y+ATVLH KVNHSWALLRFE+ A  TV P +I+Y
Sbjct: 66  GDKYHLLVKVDEFILAPSFPKYEKLYMATVLHNKVNHSWALLRFENVAPPTVYPPSIDY 124

BLAST of CsGy4G010005 vs. NCBI nr
Match: XP_022132557.1 (cysteine proteinase inhibitor 6-like [Momordica charantia])

HSP 1 Score: 59.3 bits (142), Expect = 2.66e-08
Identity = 40/99 (40.40%), Postives = 54/99 (54.55%), Query Frame = 0

Query: 10  PNNQEDCKQISNVEYNSYLQAMGRFAVEEYTHYNKGIKRKFISVGFAVVCSVSVGEEKYH 69
           P   E  K I++     Y+Q +GRFAV E+TH   G   +FI V      S  VG +KY 
Sbjct: 22  PGGWEPIKNIND----PYVQELGRFAVMEHTHNLCGASYEFIRVVSGE--SQVVGGKKYC 81

Query: 70  LLVKVDEFILAPSVPKFEKVYVATVLHKKVNHSWALLRF 108
           L+++V E I+ PS+P   K + A +L K    SWALL F
Sbjct: 82  LVIEVKETIIIPSMPSSIKFFKAIMLDKAWEKSWALLSF 114

BLAST of CsGy4G010005 vs. NCBI nr
Match: XP_038882290.1 (cysteine proteinase inhibitor 5-like [Benincasa hispida])

HSP 1 Score: 58.5 bits (140), Expect = 5.16e-08
Identity = 39/92 (42.39%), Postives = 54/92 (58.70%), Query Frame = 0

Query: 19  ISNVEYNSYLQAMGRFAVEEYTHYNKGIKRKFISVGFAVVCSVSVGEEKYHLLVKVDEFI 78
           I N+ ++ Y+Q MGRFAV E+TH   G   KFI V       +S G+E Y L+V+V E I
Sbjct: 27  IKNI-HDPYVQEMGRFAVMEHTHKLVGASLKFICVVSGETRPMSQGKE-YRLVVEVAEQI 86

Query: 79  LA--PSVPKFEKVYVATVLHKKVNHSWALLRF 108
           +   P +    K Y+ATVL K ++ SW L+ F
Sbjct: 87  ITSPPMLSVSIKFYLATVLEKPIDRSWQLISF 116

BLAST of CsGy4G010005 vs. NCBI nr
Match: KAG6590493.1 (hypothetical protein SDJN03_15916, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 57.0 bits (136), Expect = 2.02e-07
Identity = 43/106 (40.57%), Postives = 59/106 (55.66%), Query Frame = 0

Query: 8   VVPNNQEDC--KQISNVEYNSYLQAMGRFAVEEYTHYNKGIKRKFISVGFAVVC-SVSVG 67
           V+  N + C  + I N + + Y+Q MGRFAV E+ H   G K  F+ V   V C S+ V 
Sbjct: 13  VLNQNHKQCDWELIKNKD-DPYVQEMGRFAVMEHVHGLVGAKWSFVGV---VSCESLVVP 72

Query: 68  EEK-YHLLVKVDEFILAPSVPKFEKVYVATVLHKKVNHSWALLRFE 109
           + K Y LL+KVD  I+ P  P   +VY A VL K+   SW L+ F+
Sbjct: 73  KGKLYRLLIKVDIEIVVPCHPHAIEVYEAIVLDKECERSWELMSFK 114

BLAST of CsGy4G010005 vs. ExPASy TrEMBL
Match: A0A0A0KW14 (Acetate kinase OS=Cucumis sativus OX=3659 GN=Csa_4G151760 PE=4 SV=1)

HSP 1 Score: 189 bits (480), Expect = 9.63e-60
Identity = 92/119 (77.31%), Postives = 104/119 (87.39%), Query Frame = 0

Query: 5   VEMVVPNNQEDCKQISNVEYNSYLQAMGRFAVEEYTHYNKGIKRKFISVGFAVVCSVSVG 64
           V  VVPN++EDCKQISNVEYN YLQAMG+FAVEE+TH   GI+RKFISVGFAVVCSV VG
Sbjct: 6   VATVVPNDREDCKQISNVEYNEYLQAMGKFAVEEHTHNMSGIERKFISVGFAVVCSVPVG 65

Query: 65  EEKYHLLVKVDEFILAPSVPKFEKVYVATVLHKKVNHSWALLRFEDAAASTVSPTNIEY 123
            +KYHLLVKVDEFILAPS PK+EK+Y+ATVLH KVNHSWALLRFE+ A  TV P +I+Y
Sbjct: 66  GDKYHLLVKVDEFILAPSFPKYEKLYMATVLHNKVNHSWALLRFENVAPPTVYPPSIDY 124

BLAST of CsGy4G010005 vs. ExPASy TrEMBL
Match: A0A6J1BU61 (cysteine proteinase inhibitor 6-like OS=Momordica charantia OX=3673 GN=LOC111005388 PE=4 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 1.29e-08
Identity = 40/99 (40.40%), Postives = 54/99 (54.55%), Query Frame = 0

Query: 10  PNNQEDCKQISNVEYNSYLQAMGRFAVEEYTHYNKGIKRKFISVGFAVVCSVSVGEEKYH 69
           P   E  K I++     Y+Q +GRFAV E+TH   G   +FI V      S  VG +KY 
Sbjct: 22  PGGWEPIKNIND----PYVQELGRFAVMEHTHNLCGASYEFIRVVSGE--SQVVGGKKYC 81

Query: 70  LLVKVDEFILAPSVPKFEKVYVATVLHKKVNHSWALLRF 108
           L+++V E I+ PS+P   K + A +L K    SWALL F
Sbjct: 82  LVIEVKETIIIPSMPSSIKFFKAIMLDKAWEKSWALLSF 114

BLAST of CsGy4G010005 vs. ExPASy TrEMBL
Match: A0A6J1GCT2 (uncharacterized protein LOC111453027 OS=Cucurbita moschata OX=3662 GN=LOC111453027 PE=4 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 4.93e-06
Identity = 35/95 (36.84%), Postives = 49/95 (51.58%), Query Frame = 0

Query: 24  YNSYLQAMGRFAVEEYTHYNKGIKRKFISVGFAVVCSVSVGEEKYHLLVKVDEFILAPSV 83
           ++ Y+Q +GRFAV E+ H   G+   FI V       V  G+ KY L+V+V E  + P  
Sbjct: 29  HDQYVQELGRFAVMEHVHGLCGVGMTFICVVSGESKMVCEGK-KYSLVVEVKEVPVLPHA 88

Query: 84  PKFEKVYVATVLHKKVNHSWALLRFEDAAASTVSP 118
               K+YVA VL K+   SW LL F+      + P
Sbjct: 89  CPAIKLYVADVLDKQCAKSWELLAFKPFCLPPMGP 122

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
KAE8649343.15.86e-86100.00hypothetical protein Csa_014384 [Cucumis sativus][more]
KGN53820.11.99e-5977.31hypothetical protein Csa_014847 [Cucumis sativus][more]
XP_022132557.12.66e-0840.40cysteine proteinase inhibitor 6-like [Momordica charantia][more]
XP_038882290.15.16e-0842.39cysteine proteinase inhibitor 5-like [Benincasa hispida][more]
KAG6590493.12.02e-0740.57hypothetical protein SDJN03_15916, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
A0A0A0KW149.63e-6077.31Acetate kinase OS=Cucumis sativus OX=3659 GN=Csa_4G151760 PE=4 SV=1[more]
A0A6J1BU611.29e-0840.40cysteine proteinase inhibitor 6-like OS=Momordica charantia OX=3673 GN=LOC111005... [more]
A0A6J1GCT24.93e-0636.84uncharacterized protein LOC111453027 OS=Cucurbita moschata OX=3662 GN=LOC1114530... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.10.450.10coord: 13..110
e-value: 4.2E-8
score: 35.3
NoneNo IPR availableSUPERFAMILY54403Cystatin/monellincoord: 19..111

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy4G010005.1CsGy4G010005.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
molecular_function GO:0016301 kinase activity