CsGy4G003230 (gene) Cucumber (Gy14) v2.1

Overview


Name	CsGy4G003230
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description	ervatamin-B-like
Location	Gy14Chr4: 2037761 .. 2039086 (-)
RNA-Seq Expression	CsGy4G003230
Synteny	CsGy4G003230

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACCATGATGAAATTTCTTATTGTTTTCGTTGTTTTGATTGCTTTCACGTCTCATTTGTGTGAGAGCTTTGAGTTGGAAGGAAAGGATTTTGAATCTGAAAGAAGTCTCATGCAACTCTACAAAAGATGGAGTAGCCACCATAGAATCTCAAGAAATGCACATGAGATGCACAAACGTTTCAAGATCTTTCAAGATAATGCAAAACATGTGTTTAGAGTGAACCACATGGGAAAATCATTAAAATTGCGACTTAACCAGTTTGCTGATTTGTCTGATGATGAGTTTAGTATGATGTACGGTTCCAACATTACTCACTACAACAGCTTACATGCCAATCGTGTTGGTGGATTTATGTATGAACGAGCAATGAATATCCCATCTTCAATCGATTGGAGGCAAAGAGGAGCTGTGAATGCCATAAAAAATCAAGGCCGTTGTGGTAATTAATTTAATTTCATTTATAATCTTTCTAAAGCCTAATGCCTTCTAGCTAATAGTTACTAATGCCTTCTAATAATTATGTGTTATATGTTCGACAGGAAGTTGTTGGGCGTTTGCAGCTGTGGCTGCCGTGGAATCTATTCACCAAATAAGAACAAATGAATTAGTATCTCTATCAGAGCAAGAAGTGGTGGATTGTGATTATAAGGTCGGTGGTTGTCGTGGAGGAAATTATGACTCTGCATTTGAGTTCATAATGCAAAATGGTGGAATCACAATTGAGGAAAACTATCCATATTTTGCAGGAAATGGATATTGTCGTAGACGAGGAGTGAGTTTAAACCAATATAATTCTAATTAAACTATCTAAATTTATTATATGAGTGCATTATTGATCATAATATAATTATGTGTATTGTGTTTGATATTTCACTAATAGCCTAACAGTGAGAGAGTAACAATTGATGGATATGAGCGTGTACCTCAAAACAACGAGTATGCTTTGATGAAAGCAGTGGCACACCAACCGGTAGCAGTGTCGGTAGCTTCAAGTGGGAGTGATTTTAGATTTTACGGGGAGGCAAGTGTAGTTAATTTTGTTGTTGTTAATTATAATTTGGTGCAACATATATAATTAAAAGAAAAAATGATAATTATTTGCAGGGAATGCTTAGAGAAGATAGCTTTTGCGGATATAGAATTGACCACACGGTAGTGGTAGTTGGGTATGGAAGTGATGAAGAGGGAGATTATTGGATAATAAGAAACCAATATGGAACTCAATGGGGAATGAATGGTTACATGAAGATGCAACGAGGAACACGAAACCCACAAGGTGTATGTGGAATGGCGATGCAACCTTCCTTTCCCGTCAAGTATTGA

mRNA sequence

ATGACCATGATGAAATTTCTTATTGTTTTCGTTGTTTTGATTGCTTTCACGTCTCATTTGTGTGAGAGCTTTGAGTTGGAAGGAAAGGATTTTGAATCTGAAAGAAGTCTCATGCAACTCTACAAAAGATGGAGTAGCCACCATAGAATCTCAAGAAATGCACATGAGATGCACAAACGTTTCAAGATCTTTCAAGATAATGCAAAACATGTGTTTAGAGTGAACCACATGGGAAAATCATTAAAATTGCGACTTAACCAGTTTGCTGATTTGTCTGATGATGAGTTTAGTATGATGTACGGTTCCAACATTACTCACTACAACAGCTTACATGCCAATCGTGTTGGTGGATTTATGTATGAACGAGCAATGAATATCCCATCTTCAATCGATTGGAGGCAAAGAGGAGCTGTGAATGCCATAAAAAATCAAGGCCGTTGTGGAAGTTGTTGGGCGTTTGCAGCTGTGGCTGCCGTGGAATCTATTCACCAAATAAGAACAAATGAATTAGTATCTCTATCAGAGCAAGAAGTGGTGGATTGTGATTATAAGGTCGGTGGTTGTCGTGGAGGAAATTATGACTCTGCATTTGAGTTCATAATGCAAAATGGTGGAATCACAATTGAGGAAAACTATCCATATTTTGCAGGAAATGGATATTGTCGTAGACGAGGACCTAACAGTGAGAGAGTAACAATTGATGGATATGAGCGTGTACCTCAAAACAACGAGTATGCTTTGATGAAAGCAGTGGCACACCAACCGGTAGCAGTGTCGGTAGCTTCAAGTGGGAGTGATTTTAGATTTTACGGGGAGGGAATGCTTAGAGAAGATAGCTTTTGCGGATATAGAATTGACCACACGGTAGTGGTAGTTGGGTATGGAAGTGATGAAGAGGGAGATTATTGGATAATAAGAAACCAATATGGAACTCAATGGGGAATGAATGGTTACATGAAGATGCAACGAGGAACACGAAACCCACAAGGTGTATGTGGAATGGCGATGCAACCTTCCTTTCCCGTCAAGTATTGA

Coding sequence (CDS)

ATGACCATGATGAAATTTCTTATTGTTTTCGTTGTTTTGATTGCTTTCACGTCTCATTTGTGTGAGAGCTTTGAGTTGGAAGGAAAGGATTTTGAATCTGAAAGAAGTCTCATGCAACTCTACAAAAGATGGAGTAGCCACCATAGAATCTCAAGAAATGCACATGAGATGCACAAACGTTTCAAGATCTTTCAAGATAATGCAAAACATGTGTTTAGAGTGAACCACATGGGAAAATCATTAAAATTGCGACTTAACCAGTTTGCTGATTTGTCTGATGATGAGTTTAGTATGATGTACGGTTCCAACATTACTCACTACAACAGCTTACATGCCAATCGTGTTGGTGGATTTATGTATGAACGAGCAATGAATATCCCATCTTCAATCGATTGGAGGCAAAGAGGAGCTGTGAATGCCATAAAAAATCAAGGCCGTTGTGGAAGTTGTTGGGCGTTTGCAGCTGTGGCTGCCGTGGAATCTATTCACCAAATAAGAACAAATGAATTAGTATCTCTATCAGAGCAAGAAGTGGTGGATTGTGATTATAAGGTCGGTGGTTGTCGTGGAGGAAATTATGACTCTGCATTTGAGTTCATAATGCAAAATGGTGGAATCACAATTGAGGAAAACTATCCATATTTTGCAGGAAATGGATATTGTCGTAGACGAGGACCTAACAGTGAGAGAGTAACAATTGATGGATATGAGCGTGTACCTCAAAACAACGAGTATGCTTTGATGAAAGCAGTGGCACACCAACCGGTAGCAGTGTCGGTAGCTTCAAGTGGGAGTGATTTTAGATTTTACGGGGAGGGAATGCTTAGAGAAGATAGCTTTTGCGGATATAGAATTGACCACACGGTAGTGGTAGTTGGGTATGGAAGTGATGAAGAGGGAGATTATTGGATAATAAGAAACCAATATGGAACTCAATGGGGAATGAATGGTTACATGAAGATGCAACGAGGAACACGAAACCCACAAGGTGTATGTGGAATGGCGATGCAACCTTCCTTTCCCGTCAAGTATTGA

Protein sequence

MTMMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKRFKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNSLHANRVGGFMYERAMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSEQEVVDCDYKVGGCRGGNYDSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGYERVPQNNEYALMKAVAHQPVAVSVASSGSDFRFYGEGMLREDSFCGYRIDHTVVVVGYGSDEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY*

Homology

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BLAST of CsGy4G003230 vs. ExPASy Swiss-Prot
Match: O65039 (Vignain OS=Ricinus communis OX=3988 GN=CYSEP PE=1 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 4.8e-95
Identity = 172/346 (49.71%), Postives = 238/346 (68.79%), Query Frame = 0


Query: 3   MMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKRFK 62
           M KF I+  + +A    + ESF+   K+ ESE SL  LY+RW SHH +SR+ HE  KRF 
Sbjct: 1   MQKF-ILLALSLALVLAITESFDFHEKELESEESLWGLYERWRSHHTVSRSLHEKQKRFN 60

Query: 63  IFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITHYNSLHANRVGG--FM 122
           +F+ NA HV   N M K  KL+LN+FAD+++ EF   Y GS + H+        G   FM
Sbjct: 61  VFKHNAMHVHNANKMDKPYKLKLNKFADMTNHEFRNTYSGSKVKHHRMFRGGPRGNGTFM 120

Query: 123 YERAMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSEQEVV 182
           YE+   +P+S+DWR++GAV ++K+QG+CGSCWAF+ + AVE I+QI+TN+LVSLSEQE+V
Sbjct: 121 YEKVDTVPASVDWRKKGAVTSVKDQGQCGSCWAFSTIVAVEGINQIKTNKLVSLSEQELV 180

Query: 183 DCDYKVG-GCRGGNYDSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGYER 242
           DCD     GC GG  D AFEFI Q GGIT E NYPY A +G C     N+  V+IDG+E 
Sbjct: 181 DCDTDQNQGCNGGLMDYAFEFIKQRGGITTEANYPYEAYDGTCDVSKENAPAVSIDGHEN 240

Query: 243 VPQNNEYALMKAVAHQPVAVSVASSGSDFRFYGEGMLREDSFCGYRIDHTVVVVGYGSDE 302
           VP+N+E AL+KAVA+QPV+V++ + GSDF+FY EG+      CG  +DH V +VGYG+  
Sbjct: 241 VPENDENALLKAVANQPVSVAIDAGGSDFQFYSEGVF--TGSCGTELDHGVAIVGYGTTI 300

Query: 303 EG-DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 344
           +G  YW ++N +G +WG  GY++M+RG  + +G+CG+AM+ S+P+K
Sbjct: 301 DGTKYWTVKNSWGPEWGEKGYIRMERGISDKEGLCGIAMEASYPIK 343

[Close]

BLAST of CsGy4G003230 vs. ExPASy Swiss-Prot
Match: P12412 (Vignain OS=Vigna mungo OX=3915 PE=1 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 6.2e-95
Identity = 171/345 (49.57%), Postives = 236/345 (68.41%), Query Frame = 0


Query: 4   MKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKRFKI 63
           MK L+  V+ ++    +  SF+   KD ESE SL  LY+RW SHH +SR+  E HKRF +
Sbjct: 3   MKKLLWVVLSLSLVLGVANSFDFHEKDLESEESLWDLYERWRSHHTVSRSLGEKHKRFNV 62

Query: 64  FQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITHYNSLHANRVGG--FMY 123
           F+ N  HV   N M K  KL+LN+FAD+++ EF   Y GS + H+     ++ G   FMY
Sbjct: 63  FKANVMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVNHHKMFRGSQHGSGTFMY 122

Query: 124 ERAMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSEQEVVD 183
           E+  ++P+S+DWR++GAV  +K+QG+CGSCWAF+ + AVE I+QI+TN+LVSLSEQE+VD
Sbjct: 123 EKVGSVPASVDWRKKGAVTDVKDQGQCGSCWAFSTIVAVEGINQIKTNKLVSLSEQELVD 182

Query: 184 CDYKVG-GCRGGNYDSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGYERV 243
           CD +   GC GG  +SAFEFI Q GGIT E NYPY A  G C     N   V+IDG+E V
Sbjct: 183 CDKEENQGCNGGLMESAFEFIKQKGGITTESNYPYTAQEGTCDESKVNDLAVSIDGHENV 242

Query: 244 PQNNEYALMKAVAHQPVAVSVASSGSDFRFYGEGMLREDSFCGYRIDHTVVVVGYGSDEE 303
           P N+E AL+KAVA+QPV+V++ + GSDF+FY EG+   D  C   ++H V +VGYG+  +
Sbjct: 243 PVNDENALLKAVANQPVSVAIDAGGSDFQFYSEGVFTGD--CNTDLNHGVAIVGYGTTVD 302

Query: 304 G-DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 344
           G +YWI+RN +G +WG  GY++MQR     +G+CG+AM  S+P+K
Sbjct: 303 GTNYWIVRNSWGPEWGEQGYIRMQRNISKKEGLCGIAMMASYPIK 345

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BLAST of CsGy4G003230 vs. ExPASy Swiss-Prot
Match: P25803 (Vignain OS=Phaseolus vulgaris OX=3885 PE=2 SV=2)

HSP 1 Score: 344.4 bits (882), Expect = 1.5e-93
Identity = 170/344 (49.42%), Postives = 233/344 (67.73%), Query Frame = 0


Query: 5   KFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKRFKIF 64
           K L+  V+  +    +  SF+   KD  SE SL  LY+RW SHH +SR+  E HKRF +F
Sbjct: 4   KKLLWVVLSFSLVLGVANSFDFHDKDLASEESLWDLYERWRSHHTVSRSLGEKHKRFNVF 63

Query: 65  QDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITHYNSLHA--NRVGGFMYE 124
           + N  HV   N M K  KL+LN+FAD+++ EF   Y GS + H        +  G FMYE
Sbjct: 64  KANLMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVNHPRMFRGTPHENGAFMYE 123

Query: 125 RAMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSEQEVVDC 184
           + +++P S+DWR++GAV  +K+QG+CGSCWAF+ V AVE I+QI+TN+LV+LSEQE+VDC
Sbjct: 124 KVVSVPPSVDWRKKGAVTDVKDQGQCGSCWAFSTVVAVEGINQIKTNKLVALSEQELVDC 183

Query: 185 DYKVG-GCRGGNYDSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGYERVP 244
           D +   GC GG  +SAFEFI Q GGIT E NYPY A  G C     N   V+IDG+E VP
Sbjct: 184 DKEENQGCNGGLMESAFEFIKQKGGITTESNYPYKAQEGTCDASKVNDLAVSIDGHENVP 243

Query: 245 QNNEYALMKAVAHQPVAVSVASSGSDFRFYGEGMLREDSFCGYRIDHTVVVVGYGSDEEG 304
            N+E AL+KAVA+QPV+V++ + GSDF+FY EG+   D  C   ++H V +VGYG+  +G
Sbjct: 244 ANDEDALLKAVANQPVSVAIDAGGSDFQFYSEGVFTGD--CSTDLNHGVAIVGYGTTVDG 303

Query: 305 -DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 344
            +YWI+RN +G +WG +GY++MQR     +G+CG+AM PS+P+K
Sbjct: 304 TNYWIVRNSWGPEWGEHGYIRMQRNISKKEGLCGIAMLPSYPIK 345

[Close]

BLAST of CsGy4G003230 vs. ExPASy Swiss-Prot
Match: Q9STL5 (KDEL-tailed cysteine endopeptidase CEP3 OS=Arabidopsis thaliana OX=3702 GN=CEP3 PE=2 SV=1)

HSP 1 Score: 341.7 bits (875), Expect = 1.0e-92
Identity = 169/344 (49.13%), Postives = 235/344 (68.31%), Query Frame = 0


Query: 8   IVFVVLIAFTSHL--CESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKRFKIFQ 67
           + F+VLI+F S L   + F+ + K+ E+E ++ +LY+RW  HH +SR +HE  KRF +F+
Sbjct: 3   LFFIVLISFLSLLQASKGFDFDEKELETEENVWKLYERWRGHHSVSRASHEAIKRFNVFR 62

Query: 68  DNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITHYNSLHANR--VGGFMYER 127
            N  HV R N   K  KL++N+FAD++  EF   Y GSN+ H+  L   +   GGFMYE 
Sbjct: 63  HNVLHVHRTNKKNKPYKLKINRFADITHHEFRSSYAGSNVKHHRMLRGPKRGSGGFMYEN 122

Query: 128 AMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSEQEVVDCD 187
              +PSS+DWR++GAV  +KNQ  CGSCWAF+ VAAVE I++IRTN+LVSLSEQE+VDCD
Sbjct: 123 VTRVPSSVDWREKGAVTEVKNQQDCGSCWAFSTVAAVEGINKIRTNKLVSLSEQELVDCD 182

Query: 188 YKVG-GCRGGNYDSAFEFIMQNGGITIEENYPYFAGN-GYCRRRGPNSERVTIDGYERVP 247
            +   GC GG  + AFEFI  NGGI  EE YPY + +  +CR      E VTIDG+E VP
Sbjct: 183 TEENQGCAGGLMEPAFEFIKNNGGIKTEETYPYDSSDVQFCRANSIGGETVTIDGHEHVP 242

Query: 248 QNNEYALMKAVAHQPVAVSVASSGSDFRFYGEGMLREDSFCGYRIDHTVVVVGYGSDEEG 307
           +N+E  L+KAVAHQPV+V++ +  SDF+ Y EG+   +  CG +++H VV+VGYG  + G
Sbjct: 243 ENDEEELLKAVAHQPVSVAIDAGSSDFQLYSEGVFIGE--CGTQLNHGVVIVGYGETKNG 302

Query: 308 -DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 344
             YWI+RN +G +WG  GY++++RG    +G CG+AM+ S+P K
Sbjct: 303 TKYWIVRNSWGPEWGEGGYVRIERGISENEGRCGIAMEASYPTK 344

[Close]

BLAST of CsGy4G003230 vs. ExPASy Swiss-Prot
Match: Q9STL4 (KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana OX=3702 GN=CEP2 PE=1 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 2.2e-92
Identity = 172/347 (49.57%), Postives = 232/347 (66.86%), Query Frame = 0


Query: 3   MMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKRFK 62
           M K L++F+  +      C  F+ + K+ ESE  L  LY RW SHH + R+ +E  KRF 
Sbjct: 1   MKKLLLIFLFSLVILQTAC-GFDYDDKEIESEEGLSTLYDRWRSHHSVPRSLNEREKRFN 60

Query: 63  IFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITHYNSLHANRVGG--FM 122
           +F+ N  HV   N   +S KL+LN+FADL+ +EF   Y GSNI H+  L   + G   FM
Sbjct: 61  VFRHNVMHVHNTNKKNRSYKLKLNKFADLTINEFKNAYTGSNIKHHRMLQGPKRGSKQFM 120

Query: 123 Y--ERAMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSEQE 182
           Y  E    +PSS+DWR++GAV  IKNQG+CGSCWAF+ VAAVE I++I+TN+LVSLSEQE
Sbjct: 121 YDHENLSKLPSSVDWRKKGAVTEIKNQGKCGSCWAFSTVAAVEGINKIKTNKLVSLSEQE 180

Query: 183 VVDCDYKVG-GCRGGNYDSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGY 242
           +VDCD K   GC GG  + AFEFI +NGGIT E++YPY   +G C     N   VTIDG+
Sbjct: 181 LVDCDTKQNEGCNGGLMEIAFEFIKKNGGITTEDSYPYEGIDGKCDASKDNGVLVTIDGH 240

Query: 243 ERVPQNNEYALMKAVAHQPVAVSVASSGSDFRFYGEGMLREDSFCGYRIDHTVVVVGYGS 302
           E VP+N+E AL+KAVA+QPV+V++ +  SDF+FY EG+      CG  ++H V  VGYGS
Sbjct: 241 EDVPENDENALLKAVANQPVSVAIDAGSSDFQFYSEGVF--TGSCGTELNHGVAAVGYGS 300

Query: 303 DEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 344
           +    YWI+RN +G +WG  GY+K++R    P+G CG+AM+ S+P+K
Sbjct: 301 ERGKKYWIVRNSWGAEWGEGGYIKIEREIDEPEGRCGIAMEASYPIK 344

[Close]

BLAST of CsGy4G003230 vs. NCBI nr
Match: XP_031740474.1 (ervatamin-B [Cucumis sativus] >KAE8649110.1 hypothetical protein Csa_014565 [Cucumis sativus])

HSP 1 Score: 710 bits (1832), Expect = 2.48e-258
Identity = 343/344 (99.71%), Postives = 343/344 (99.71%), Query Frame = 0


Query: 1   MTMMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKR 60
           MTMMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKR
Sbjct: 1   MTMMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKR 60

Query: 61  FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNSLHANRVGGFMY 120
           FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNSLHANRVGGFMY
Sbjct: 61  FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNSLHANRVGGFMY 120

Query: 121 ERAMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSEQEVVD 180
           ERAMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSEQEVVD
Sbjct: 121 ERAMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSEQEVVD 180

Query: 181 CDYKVGGCRGGNYDSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGYERVP 240
           CDYKVGGCRGGNYDSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGYE VP
Sbjct: 181 CDYKVGGCRGGNYDSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGYECVP 240

Query: 241 QNNEYALMKAVAHQPVAVSVASSGSDFRFYGEGMLREDSFCGYRIDHTVVVVGYGSDEEG 300
           QNNEYALMKAVAHQPVAVSVASSGSDFRFYGEGMLREDSFCGYRIDHTVVVVGYGSDEEG
Sbjct: 241 QNNEYALMKAVAHQPVAVSVASSGSDFRFYGEGMLREDSFCGYRIDHTVVVVGYGSDEEG 300

Query: 301 DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 344
           DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY
Sbjct: 301 DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 344

[Close]

BLAST of CsGy4G003230 vs. NCBI nr
Match: XP_031740503.1 (ervatamin-B [Cucumis sativus])

HSP 1 Score: 676 bits (1744), Expect = 7.22e-245
Identity = 327/347 (94.24%), Postives = 336/347 (96.83%), Query Frame = 0


Query: 1   MTMMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKR 60
           MTMMKFLIVFVVLIAF SHLCE F+LE KDFESE+SLMQLYKRWSSHHRISRNAHEMHKR
Sbjct: 1   MTMMKFLIVFVVLIAFASHLCEGFDLERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKR 60

Query: 61  FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNSLHAN---RVGG 120
           FKIFQDNAK VF+VNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYN+LHA    RVGG
Sbjct: 61  FKIFQDNAKRVFKVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNNLHAKAGGRVGG 120

Query: 121 FMYERAMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSEQE 180
           FMYERAMNIP SIDWR++GAVNAIKNQG CGSCWAFAAVAAVESIHQI+TNELVSLSEQE
Sbjct: 121 FMYERAMNIPFSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSEQE 180

Query: 181 VVDCDYKVGGCRGGNYDSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGYE 240
           VVDCDYKVGGCRGG+Y+SAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGYE
Sbjct: 181 VVDCDYKVGGCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGYE 240

Query: 241 RVPQNNEYALMKAVAHQPVAVSVASSGSDFRFYGEGMLREDSFCGYRIDHTVVVVGYGSD 300
           RVPQNNEYALMKAVAHQPVAVSVASSGSDFRFYGEGMLRE SFCGYRIDHTVVVVGYGSD
Sbjct: 241 RVPQNNEYALMKAVAHQPVAVSVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVGYGSD 300

Query: 301 EEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 344
           EEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY
Sbjct: 301 EEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 347

[Close]

BLAST of CsGy4G003230 vs. NCBI nr
Match: XP_031739597.1 (ervatamin-B-like [Cucumis sativus] >KAE8649107.1 hypothetical protein Csa_014529 [Cucumis sativus])

HSP 1 Score: 675 bits (1742), Expect = 1.57e-244
Identity = 327/349 (93.70%), Postives = 338/349 (96.85%), Query Frame = 0


Query: 1   MTMMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKR 60
           MTMMKFLIVFVVLIAFTSHLCESFELE KDFESE+SLMQLYKRWSSHHRISRNAHEMHKR
Sbjct: 1   MTMMKFLIVFVVLIAFTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKR 60

Query: 61  FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNSLHAN-----RV 120
           FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHY++LHA      RV
Sbjct: 61  FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYDNLHAKAGGGGRV 120

Query: 121 GGFMYERAMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSE 180
           GGFMYERA  IPSSIDWR++GAVNAIKNQG CGSCWAFAAVAAVESIHQI+TNELVSLSE
Sbjct: 121 GGFMYERARYIPSSIDWREKGAVNAIKNQGLCGSCWAFAAVAAVESIHQIKTNELVSLSE 180

Query: 181 QEVVDCDYKVGGCRGGNYDSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDG 240
           QEVVDCDYKVGGCRGG+Y+SAFEFIMQNGGITIEENYPYFAGNGYCRRRGPN+ERVTIDG
Sbjct: 181 QEVVDCDYKVGGCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNNERVTIDG 240

Query: 241 YERVPQNNEYALMKAVAHQPVAVSVASSGSDFRFYGEGMLREDSFCGYRIDHTVVVVGYG 300
           YERVP+NNEYALMKAVAHQPVAV+VASSGSDFRFYGEGMLRE SFCGYRIDHTVVVVGYG
Sbjct: 241 YERVPRNNEYALMKAVAHQPVAVAVASSGSDFRFYGEGMLREGSFCGYRIDHTVVVVGYG 300

Query: 301 SDEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 344
           SDEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY
Sbjct: 301 SDEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 349

[Close]

BLAST of CsGy4G003230 vs. NCBI nr
Match: KAE8649111.1 (hypothetical protein Csa_014425 [Cucumis sativus])

HSP 1 Score: 647 bits (1668), Expect = 2.38e-233
Identity = 320/350 (91.43%), Postives = 329/350 (94.00%), Query Frame = 0


Query: 1   MTMMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKR 60
           MTMMKFLIVFVVLIAF SHLCE F+LE KDFESE+SLMQLYKRWSSHHRISRNAHEMHKR
Sbjct: 1   MTMMKFLIVFVVLIAFASHLCEGFDLERKDFESEKSLMQLYKRWSSHHRISRNAHEMHKR 60

Query: 61  FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNSLHAN---RVGG 120
           FKIFQDNAK VF+VNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYN+LHA    RVGG
Sbjct: 61  FKIFQDNAKRVFKVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNNLHAKAGGRVGG 120

Query: 121 FMYERAMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSEQE 180
           FMYERAMNIP SIDWR++GAVNAIKNQG C       AVAAVESIHQI+TNELVSLSEQE
Sbjct: 121 FMYERAMNIPFSIDWREKGAVNAIKNQGLC-------AVAAVESIHQIKTNELVSLSEQE 180

Query: 181 VVDCDYKVGGCRGGNYDSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGYE 240
           VVDCDYKVGGCRGG+Y+SAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGYE
Sbjct: 181 VVDCDYKVGGCRGGDYNSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGYE 240

Query: 241 RVPQNNEYALMKAVAHQPVAVSVASSGSDFRFYGE---GMLREDSFCGYRIDHTVVVVGY 300
           RVPQNNEYALMKAVAHQPVAVSVASSGSDFRFYGE   GMLRE SFCGYRIDHTVVVVGY
Sbjct: 241 RVPQNNEYALMKAVAHQPVAVSVASSGSDFRFYGEASVGMLREGSFCGYRIDHTVVVVGY 300

Query: 301 GSDEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 344
           GSDEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY
Sbjct: 301 GSDEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 343

[Close]

BLAST of CsGy4G003230 vs. NCBI nr
Match: XP_008454483.1 (PREDICTED: ervatamin-B-like [Cucumis melo])

HSP 1 Score: 600 bits (1546), Expect = 1.12e-214
Identity = 285/346 (82.37%), Postives = 319/346 (92.20%), Query Frame = 0


Query: 2   TMMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKRF 61
           T+MKFLIV +VLIA TSHLCESFELE KDFESE+SLMQLYKRWSSHHRISRNA+EMHKRF
Sbjct: 3   TVMKFLIVPLVLIALTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKRF 62

Query: 62  KIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNSLHAN--RVGGFM 121
           K+F+DNAKHVF+ NHMG+SLKL+LNQFAD+SDDEFS ++GSNIT+Y +LHA    VGGFM
Sbjct: 63  KVFKDNAKHVFKKNHMGRSLKLQLNQFADMSDDEFSSIHGSNITYYKNLHAKTGHVGGFM 122

Query: 122 YERAMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSEQEVV 181
           YE A  IPSSIDWR++GAVNAIKNQG CGSCWAFAAVAAVESIHQI+TNELVSLSEQEVV
Sbjct: 123 YEHAKEIPSSIDWRKKGAVNAIKNQGGCGSCWAFAAVAAVESIHQIKTNELVSLSEQEVV 182

Query: 182 DCDYKVGGCRGGNYDSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGYERV 241
           DCDY+ GGCRGG+Y+SAFEF+M+NGGIT+E+NYPY+ G+GYCRRRG  +ERV IDGYE V
Sbjct: 183 DCDYRDGGCRGGHYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVKIDGYENV 242

Query: 242 PQNNEYALMKAVAHQPVAVSVASSGSDFRFYGEGMLREDSFCGYRIDHTVVVVGYGSDEE 301
           P+NNE+ALMKAVAHQPVAV++ASSGSDFRFYG+GM  E  FCGY IDHTVVVVGYGSDEE
Sbjct: 243 PRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQGMFTEQDFCGYNIDHTVVVVGYGSDEE 302

Query: 302 -GDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 344
            GDYWIIRNQYGTQWGMNGYMKMQRG RNPQGVCGMAMQP++PVKY
Sbjct: 303 DGDYWIIRNQYGTQWGMNGYMKMQRGARNPQGVCGMAMQPAYPVKY 348

[Close]

BLAST of CsGy4G003230 vs. ExPASy TrEMBL
Match: A0A1S3BYQ3 (ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103494879 PE=3 SV=1)

HSP 1 Score: 600 bits (1546), Expect = 5.40e-215
Identity = 285/346 (82.37%), Postives = 319/346 (92.20%), Query Frame = 0


Query: 2   TMMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKRF 61
           T+MKFLIV +VLIA TSHLCESFELE KDFESE+SLMQLYKRWSSHHRISRNA+EMHKRF
Sbjct: 3   TVMKFLIVPLVLIALTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKRF 62

Query: 62  KIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNSLHAN--RVGGFM 121
           K+F+DNAKHVF+ NHMG+SLKL+LNQFAD+SDDEFS ++GSNIT+Y +LHA    VGGFM
Sbjct: 63  KVFKDNAKHVFKKNHMGRSLKLQLNQFADMSDDEFSSIHGSNITYYKNLHAKTGHVGGFM 122

Query: 122 YERAMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSEQEVV 181
           YE A  IPSSIDWR++GAVNAIKNQG CGSCWAFAAVAAVESIHQI+TNELVSLSEQEVV
Sbjct: 123 YEHAKEIPSSIDWRKKGAVNAIKNQGGCGSCWAFAAVAAVESIHQIKTNELVSLSEQEVV 182

Query: 182 DCDYKVGGCRGGNYDSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGYERV 241
           DCDY+ GGCRGG+Y+SAFEF+M+NGGIT+E+NYPY+ G+GYCRRRG  +ERV IDGYE V
Sbjct: 183 DCDYRDGGCRGGHYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVKIDGYENV 242

Query: 242 PQNNEYALMKAVAHQPVAVSVASSGSDFRFYGEGMLREDSFCGYRIDHTVVVVGYGSDEE 301
           P+NNE+ALMKAVAHQPVAV++ASSGSDFRFYG+GM  E  FCGY IDHTVVVVGYGSDEE
Sbjct: 243 PRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQGMFTEQDFCGYNIDHTVVVVGYGSDEE 302

Query: 302 -GDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 344
            GDYWIIRNQYGTQWGMNGYMKMQRG RNPQGVCGMAMQP++PVKY
Sbjct: 303 DGDYWIIRNQYGTQWGMNGYMKMQRGARNPQGVCGMAMQPAYPVKY 348

[Close]

BLAST of CsGy4G003230 vs. ExPASy TrEMBL
Match: A0A5A7TM64 (Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G003430 PE=3 SV=1)

HSP 1 Score: 599 bits (1544), Expect = 1.09e-214
Identity = 285/346 (82.37%), Postives = 318/346 (91.91%), Query Frame = 0


Query: 2   TMMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKRF 61
           T+MKFLIV  VLIA TSHLCESFELE KDFESE+SLMQLYKRWSSHHRISRNA+EMHKRF
Sbjct: 3   TVMKFLIVPFVLIALTSHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKRF 62

Query: 62  KIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNSLHAN--RVGGFM 121
           K+F+DNAKHVF+ NHMG+SLKL+LNQFAD+SDDEFS ++GSNIT+Y +LHA    VGGFM
Sbjct: 63  KVFKDNAKHVFKKNHMGRSLKLQLNQFADMSDDEFSSIHGSNITYYKNLHAKTGHVGGFM 122

Query: 122 YERAMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSEQEVV 181
           YE A  IPSSIDWR++GAVNAIKNQG CGSCWAFAAVAAVESIHQI+TNELVSLSEQEVV
Sbjct: 123 YEHAKEIPSSIDWRKKGAVNAIKNQGGCGSCWAFAAVAAVESIHQIKTNELVSLSEQEVV 182

Query: 182 DCDYKVGGCRGGNYDSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGYERV 241
           DCDY+ GGCRGG+Y+SAFEF+M+NGGIT+E+NYPY+ G+GYCRRRG  +ERV IDGYE V
Sbjct: 183 DCDYRDGGCRGGHYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVKIDGYENV 242

Query: 242 PQNNEYALMKAVAHQPVAVSVASSGSDFRFYGEGMLREDSFCGYRIDHTVVVVGYGSDEE 301
           P+NNE+ALMKAVAHQPVAV++ASSGSDFRFYG+GM  E  FCGY IDHTVVVVGYGSDEE
Sbjct: 243 PRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQGMFTEQDFCGYNIDHTVVVVGYGSDEE 302

Query: 302 -GDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 344
            GDYWIIRNQYGTQWGMNGYMKMQRG RNPQGVCGMAMQP++PVKY
Sbjct: 303 DGDYWIIRNQYGTQWGMNGYMKMQRGARNPQGVCGMAMQPAYPVKY 348

[Close]

BLAST of CsGy4G003230 vs. ExPASy TrEMBL
Match: A0A1S3BYU0 (ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103494878 PE=3 SV=1)

HSP 1 Score: 593 bits (1528), Expect = 2.88e-212
Identity = 282/347 (81.27%), Postives = 320/347 (92.22%), Query Frame = 0


Query: 1   MTMMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKR 60
           M +MKFLIV +VLIAFT HLCESFELE KDFESE+SLMQLYKRWSSHHRISRNA+EMHKR
Sbjct: 1   MAVMKFLIVPLVLIAFTFHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKR 60

Query: 61  FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNSLHAN--RVGGF 120
           FK+F+DNAK+VF+ NHMG+SLKL+LNQFAD+SDDEFS ++GSNIT+Y +LHA   RVGGF
Sbjct: 61  FKVFKDNAKYVFKKNHMGRSLKLQLNQFADMSDDEFSSIHGSNITYYKNLHAKNGRVGGF 120

Query: 121 MYERAMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSEQEV 180
           MYE A +IPSSIDWR++GAVNAIKNQGRCGSCWAFAAVAAVESIHQI+TNELVSLSEQEV
Sbjct: 121 MYEHANDIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELVSLSEQEV 180

Query: 181 VDCDYKVGGCRGGNYDSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGYER 240
           VDCDY+  GC GG Y+SAFEF+M+NGGIT+E+NYPY+ G+GYCRRRG  +ERVTIDGYE 
Sbjct: 181 VDCDYRDSGCLGGFYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVTIDGYEN 240

Query: 241 VPQNNEYALMKAVAHQPVAVSVASSGSDFRFYGEGMLREDSFCGYRIDHTVVVVGYGSDE 300
           VP+NNE+ALMKAVAHQPVAV++ASSGSDFRFYG+GM  E  FCGY IDHTVVVVGYG+DE
Sbjct: 241 VPRNNEHALMKAVAHQPVAVAIASSGSDFRFYGQGMFTEQDFCGYNIDHTVVVVGYGTDE 300

Query: 301 E-GDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 344
           E GDYWIIRNQYGTQWGMNGYMKMQRG RNPQGVCGMA+QP++PVK+
Sbjct: 301 EDGDYWIIRNQYGTQWGMNGYMKMQRGARNPQGVCGMAIQPAYPVKH 347

[Close]

BLAST of CsGy4G003230 vs. ExPASy TrEMBL
Match: A0A0A0KGB1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G490180 PE=3 SV=1)

HSP 1 Score: 579 bits (1493), Expect = 7.14e-207
Identity = 272/351 (77.49%), Postives = 313/351 (89.17%), Query Frame = 0


Query: 1   MTMMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKR 60
           MT+MKFLIV +VL+AF+ ++CESFELE KDFESE+SLMQLYKRWSSHHRISRNA+EMH R
Sbjct: 1   MTVMKFLIVPLVLVAFSCNICESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHNR 60

Query: 61  FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNSLHAN------- 120
           FK+F++NAKHVF+VN MGKSLKL+LNQFAD+SDDEF  MY SNIT+Y  LHA        
Sbjct: 61  FKVFKNNAKHVFKVNLMGKSLKLKLNQFADMSDDEFRNMYSSNITYYKDLHAKKIEATGG 120

Query: 121 RVGGFMYERAMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSL 180
           R+GGFMYE A NIPSSIDWR++GAVNAIKNQGRCGSCWAFAAVAAVESIHQI+TNELVSL
Sbjct: 121 RIGGFMYEHANNIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELVSL 180

Query: 181 SEQEVVDCDYKVGGCRGGNYDSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTI 240
           SE+EV+DCDY+ GGCRGG Y+SAFEF+M N G+TIE+NYPY+ GNGYCRRRG  ++RV I
Sbjct: 181 SEEEVLDCDYRDGGCRGGFYNSAFEFMMDNDGVTIEDNYPYYEGNGYCRRRGGRNKRVRI 240

Query: 241 DGYERVPQNNEYALMKAVAHQPVAVSVASSGSDFRFYGEGMLREDSFCGYRIDHTVVVVG 300
           DGYE VP+NNEYALMKAVAHQPVAV++AS GSDF+FYG GM  E+ FCG+ IDHTVVVVG
Sbjct: 241 DGYENVPRNNEYALMKAVAHQPVAVAIASGGSDFKFYGGGMFTENDFCGFNIDHTVVVVG 300

Query: 301 YGSDEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 344
           YG+DE+GDYWIIRNQYG +WGMNGYMKMQRG  +PQGVCGMAMQP++PVKY
Sbjct: 301 YGTDEDGDYWIIRNQYGHRWGMNGYMKMQRGAHSPQGVCGMAMQPAYPVKY 351

[Close]

BLAST of CsGy4G003230 vs. ExPASy TrEMBL
Match: A0A5D3D043 (Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001320 PE=3 SV=1)

HSP 1 Score: 574 bits (1480), Expect = 6.34e-205
Identity = 277/349 (79.37%), Postives = 315/349 (90.26%), Query Frame = 0


Query: 1   MTMMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKR 60
           M +MKFLIV +VLIAFT HLCESFELE KDFESE+SLMQLYKRWSSHHRISRNA+EMHKR
Sbjct: 1   MAVMKFLIVPLVLIAFTFHLCESFELERKDFESEKSLMQLYKRWSSHHRISRNANEMHKR 60

Query: 61  FKIFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMYGSNITHYNSLHAN--RVGGF 120
           FK+F+DNAK+VF+ NHMG+SLKL+LNQFAD+SDDEFS ++GSNIT+Y +LHA   RVGGF
Sbjct: 61  FKVFKDNAKYVFKKNHMGRSLKLQLNQFADMSDDEFSSIHGSNITYYKNLHAKNGRVGGF 120

Query: 121 MYERAMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSEQEV 180
           MYE A +IPSSIDWR++GAVNAIKNQGRCGSCWAFAAVAAVESIHQI+TNELVSLSEQEV
Sbjct: 121 MYEHANDIPSSIDWRKKGAVNAIKNQGRCGSCWAFAAVAAVESIHQIKTNELVSLSEQEV 180

Query: 181 VDCDYKVGGCRGGNYDSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGYER 240
           VDCDY+  GC GG Y+SAFEF+M+NGGIT+E+NYPY+ G+GYCRRRG  +ERVTIDGYE 
Sbjct: 181 VDCDYRDSGCLGGFYNSAFEFMMENGGITVEDNYPYYEGDGYCRRRGGYNERVTIDGYEN 240

Query: 241 VPQNNEYALMKAVAHQPVAVSVASSGSDFRF--YGEGMLREDSFCGYRIDHTVVVVGYGS 300
           VP+NNE+ALMKAVAHQPVAV++ASSG    F  Y +GM  E  FCGY IDHTVVVVGYG+
Sbjct: 241 VPRNNEHALMKAVAHQPVAVAIASSGRILNFDNYLQGMFTEQDFCGYNIDHTVVVVGYGT 300

Query: 301 DEE-GDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVKY 344
           DEE GDYWIIRNQYGTQWGMNGYMKMQRG RNPQGVCGMA+QP++PVK+
Sbjct: 301 DEEDGDYWIIRNQYGTQWGMNGYMKMQRGARNPQGVCGMAIQPAYPVKH 349

[Close]

BLAST of CsGy4G003230 vs. TAIR 10
Match: AT3G48350.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 341.7 bits (875), Expect = 7.1e-94
Identity = 169/344 (49.13%), Postives = 235/344 (68.31%), Query Frame = 0


Query: 8   IVFVVLIAFTSHL--CESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKRFKIFQ 67
           + F+VLI+F S L   + F+ + K+ E+E ++ +LY+RW  HH +SR +HE  KRF +F+
Sbjct: 3   LFFIVLISFLSLLQASKGFDFDEKELETEENVWKLYERWRGHHSVSRASHEAIKRFNVFR 62

Query: 68  DNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITHYNSLHANR--VGGFMYER 127
            N  HV R N   K  KL++N+FAD++  EF   Y GSN+ H+  L   +   GGFMYE 
Sbjct: 63  HNVLHVHRTNKKNKPYKLKINRFADITHHEFRSSYAGSNVKHHRMLRGPKRGSGGFMYEN 122

Query: 128 AMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSEQEVVDCD 187
              +PSS+DWR++GAV  +KNQ  CGSCWAF+ VAAVE I++IRTN+LVSLSEQE+VDCD
Sbjct: 123 VTRVPSSVDWREKGAVTEVKNQQDCGSCWAFSTVAAVEGINKIRTNKLVSLSEQELVDCD 182

Query: 188 YKVG-GCRGGNYDSAFEFIMQNGGITIEENYPYFAGN-GYCRRRGPNSERVTIDGYERVP 247
            +   GC GG  + AFEFI  NGGI  EE YPY + +  +CR      E VTIDG+E VP
Sbjct: 183 TEENQGCAGGLMEPAFEFIKNNGGIKTEETYPYDSSDVQFCRANSIGGETVTIDGHEHVP 242

Query: 248 QNNEYALMKAVAHQPVAVSVASSGSDFRFYGEGMLREDSFCGYRIDHTVVVVGYGSDEEG 307
           +N+E  L+KAVAHQPV+V++ +  SDF+ Y EG+   +  CG +++H VV+VGYG  + G
Sbjct: 243 ENDEEELLKAVAHQPVSVAIDAGSSDFQLYSEGVFIGE--CGTQLNHGVVIVGYGETKNG 302

Query: 308 -DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 344
             YWI+RN +G +WG  GY++++RG    +G CG+AM+ S+P K
Sbjct: 303 TKYWIVRNSWGPEWGEGGYVRIERGISENEGRCGIAMEASYPTK 344

[Close]

BLAST of CsGy4G003230 vs. TAIR 10
Match: AT3G48340.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 340.5 bits (872), Expect = 1.6e-93
Identity = 172/347 (49.57%), Postives = 232/347 (66.86%), Query Frame = 0


Query: 3   MMKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKRFK 62
           M K L++F+  +      C  F+ + K+ ESE  L  LY RW SHH + R+ +E  KRF 
Sbjct: 1   MKKLLLIFLFSLVILQTAC-GFDYDDKEIESEEGLSTLYDRWRSHHSVPRSLNEREKRFN 60

Query: 63  IFQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITHYNSLHANRVGG--FM 122
           +F+ N  HV   N   +S KL+LN+FADL+ +EF   Y GSNI H+  L   + G   FM
Sbjct: 61  VFRHNVMHVHNTNKKNRSYKLKLNKFADLTINEFKNAYTGSNIKHHRMLQGPKRGSKQFM 120

Query: 123 Y--ERAMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSEQE 182
           Y  E    +PSS+DWR++GAV  IKNQG+CGSCWAF+ VAAVE I++I+TN+LVSLSEQE
Sbjct: 121 YDHENLSKLPSSVDWRKKGAVTEIKNQGKCGSCWAFSTVAAVEGINKIKTNKLVSLSEQE 180

Query: 183 VVDCDYKVG-GCRGGNYDSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGY 242
           +VDCD K   GC GG  + AFEFI +NGGIT E++YPY   +G C     N   VTIDG+
Sbjct: 181 LVDCDTKQNEGCNGGLMEIAFEFIKKNGGITTEDSYPYEGIDGKCDASKDNGVLVTIDGH 240

Query: 243 ERVPQNNEYALMKAVAHQPVAVSVASSGSDFRFYGEGMLREDSFCGYRIDHTVVVVGYGS 302
           E VP+N+E AL+KAVA+QPV+V++ +  SDF+FY EG+      CG  ++H V  VGYGS
Sbjct: 241 EDVPENDENALLKAVANQPVSVAIDAGSSDFQFYSEGVF--TGSCGTELNHGVAAVGYGS 300

Query: 303 DEEGDYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 344
           +    YWI+RN +G +WG  GY+K++R    P+G CG+AM+ S+P+K
Sbjct: 301 ERGKKYWIVRNSWGAEWGEGGYIKIEREIDEPEGRCGIAMEASYPIK 344

[Close]

BLAST of CsGy4G003230 vs. TAIR 10
Match: AT5G50260.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 331.6 bits (849), Expect = 7.3e-91
Identity = 165/345 (47.83%), Postives = 226/345 (65.51%), Query Frame = 0


Query: 4   MKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRWSSHHRISRNAHEMHKRFKI 63
           MK  IV  + +       +  +   KD ESE SL +LY+RW SHH ++R+  E  KRF +
Sbjct: 1   MKRFIVLALCMLMVLETTKGLDFHNKDVESENSLWELYERWRSHHTVARSLEEKAKRFNV 60

Query: 64  FQDNAKHVFRVNHMGKSLKLRLNQFADLSDDEFSMMY-GSNITHYNSLHANR--VGGFMY 123
           F+ N KH+   N   KS KL+LN+F D++ +EF   Y GSNI H+      +     FMY
Sbjct: 61  FKHNVKHIHETNKKDKSYKLKLNKFGDMTSEEFRRTYAGSNIKHHRMFQGEKKATKSFMY 120

Query: 124 ERAMNIPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSEQEVVD 183
                +P+S+DWR+ GAV  +KNQG+CGSCWAF+ V AVE I+QIRT +L SLSEQE+VD
Sbjct: 121 ANVNTLPTSVDWRKNGAVTPVKNQGQCGSCWAFSTVVAVEGINQIRTKKLTSLSEQELVD 180

Query: 184 CDYKVG-GCRGGNYDSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGYERV 243
           CD     GC GG  D AFEFI + GG+T E  YPY A +  C     N+  V+IDG+E V
Sbjct: 181 CDTNQNQGCNGGLMDLAFEFIKEKGGLTSELVYPYKASDETCDTNKENAPVVSIDGHEDV 240

Query: 244 PQNNEYALMKAVAHQPVAVSVASSGSDFRFYGEGMLREDSFCGYRIDHTVVVVGYGSDEE 303
           P+N+E  LMKAVA+QPV+V++ + GSDF+FY EG+      CG  ++H V VVGYG+  +
Sbjct: 241 PKNSEDDLMKAVANQPVSVAIDAGGSDFQFYSEGVF--TGRCGTELNHGVAVVGYGTTID 300

Query: 304 G-DYWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFPVK 344
           G  YWI++N +G +WG  GY++MQRG R+ +G+CG+AM+ S+P+K
Sbjct: 301 GTKYWIVKNSWGEEWGEKGYIRMQRGIRHKEGLCGIAMEASYPLK 343

[Close]

BLAST of CsGy4G003230 vs. TAIR 10
Match: AT1G20850.1 (xylem cysteine peptidase 2 )

HSP 1 Score: 288.5 bits (737), Expect = 7.1e-78
Identity = 143/318 (44.97%), Postives = 201/318 (63.21%), Query Frame = 0


Query: 29  KDFESERSLMQLYKRW-SSHHRISRNAHEMHKRFKIFQDNAKHVFRVNHMGKSLKLRLNQ 88
           +D ES   L++L++ W S+  +      E   RF++F+DN KH+   N  GKS  L LN+
Sbjct: 39  EDLESHDKLIELFENWISNFEKAYETVEEKFLRFEVFKDNLKHIDETNKKGKSYWLGLNE 98

Query: 89  FADLSDDEFSMMY-GSNITHYNSLHANRVGGFMYERAMNIPSSIDWRQRGAVNAIKNQGR 148
           FADLS +EF  MY G                F Y     +P S+DWR++GAV  +KNQG 
Sbjct: 99  FADLSHEEFKKMYLGLKTDIVRRDEERSYAEFAYRDVEAVPKSVDWRKKGAVAEVKNQGS 158

Query: 149 CGSCWAFAAVAAVESIHQIRTNELVSLSEQEVVDCDYKV-GGCRGGNYDSAFEFIMQNGG 208
           CGSCWAF+ VAAVE I++I T  L +LSEQE++DCD     GC GG  D AFE+I++NGG
Sbjct: 159 CGSCWAFSTVAAVEGINKIVTGNLTTLSEQELIDCDTTYNNGCNGGLMDYAFEYIVKNGG 218

Query: 209 ITIEENYPYFAGNGYCRRRGPNSERVTIDGYERVPQNNEYALMKAVAHQPVAVSVASSGS 268
           +  EE+YPY    G C  +   SE VTI+G++ VP N+E +L+KA+AHQP++V++ +SG 
Sbjct: 219 LRKEEDYPYSMEEGTCEMQKDESETVTINGHQDVPTNDEKSLLKALAHQPLSVAIDASGR 278

Query: 269 DFRFYGEGMLREDSFCGYRIDHTVVVVGYGSDEEGDYWIIRNQYGTQWGMNGYMKMQRGT 328
           +F+FY  G+   D  CG  +DH V  VGYGS +  DY I++N +G +WG  GY++++R T
Sbjct: 279 EFQFYSGGVF--DGRCGVDLDHGVAAVGYGSSKGSDYIIVKNSWGPKWGEKGYIRLKRNT 338

Query: 329 RNPQGVCGMAMQPSFPVK 344
             P+G+CG+    SFP K
Sbjct: 339 GKPEGLCGINKMASFPTK 354

[Close]

BLAST of CsGy4G003230 vs. TAIR 10
Match: AT5G45890.1 (senescence-associated gene 12 )

HSP 1 Score: 280.0 bits (715), Expect = 2.5e-75
Identity = 143/346 (41.33%), Postives = 217/346 (62.72%), Query Frame = 0


Query: 4   MKFLIVFVVLIAFTSHLCESFELEGKDFESERSLMQLYKRW-SSHHRISRNAHEMHKRFK 63
           +K + +F+ +  F+S  C S  L  +  ++E  + + +  W + H R+  +  E + R+ 
Sbjct: 3   LKHMQIFLFVAIFSS-FCFSITL-SRPLDNELIMQKRHIEWMTKHGRVYADVKEENNRYV 62

Query: 64  IFQDNAKHVFRVNHM--GKSLKLRLNQFADLSDDEFSMMYG--SNITHYNSLHANRVGGF 123
           +F++N + +  +N +  G++ KL +NQFADL++DEF  MY     ++  +S    ++  F
Sbjct: 63  VFKNNVERIEHLNSIPAGRTFKLAVNQFADLTNDEFRSMYTGFKGVSALSSQSQTKMSPF 122

Query: 124 MYERAMN--IPSSIDWRQRGAVNAIKNQGRCGSCWAFAAVAAVESIHQIRTNELVSLSEQ 183
            Y+   +  +P S+DWR++GAV  IKNQG CG CWAF+AVAA+E   QI+  +L+SLSEQ
Sbjct: 123 RYQNVSSGALPVSVDWRKKGAVTPIKNQGSCGCCWAFSAVAAIEGATQIKKGKLISLSEQ 182

Query: 184 EVVDCDYKVGGCRGGNYDSAFEFIMQNGGITIEENYPYFAGNGYCRRRGPNSERVTIDGY 243
           ++VDCD    GC GG  D+AFE I   GG+T E NYPY   +  C  +  N +  +I GY
Sbjct: 183 QLVDCDTNDFGCEGGLMDTAFEHIKATGGLTTESNYPYKGEDATCNSKKTNPKATSITGY 242

Query: 244 ERVPQNNEYALMKAVAHQPVAVSVASSGSDFRFYGEGMLREDSFCGYRIDHTVVVVGYGS 303
           E VP N+E ALMKAVAHQPV+V +   G DF+FY  G+   +  C   +DH V  +GYG 
Sbjct: 243 EDVPVNDEQALMKAVAHQPVSVGIEGGGFDFQFYSSGVFTGE--CTTYLDHAVTAIGYGE 302

Query: 304 DEEGD-YWIIRNQYGTQWGMNGYMKMQRGTRNPQGVCGMAMQPSFP 342
              G  YWII+N +GT+WG +GYM++Q+  ++ QG+CG+AM+ S+P
Sbjct: 303 STNGSKYWIIKNSWGTKWGESGYMRIQKDVKDKQGLCGLAMKASYP 344

The following BLAST results are available for this feature:

BLAST of CsGy4G003230 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O65039	4.8e-95	49.71	Vignain OS=Ricinus communis OX=3988 GN=CYSEP PE=1 SV=1	[more]
P12412	6.2e-95	49.57	Vignain OS=Vigna mungo OX=3915 PE=1 SV=1	[more]
P25803	1.5e-93	49.42	Vignain OS=Phaseolus vulgaris OX=3885 PE=2 SV=2	[more]
Q9STL5	1.0e-92	49.13	KDEL-tailed cysteine endopeptidase CEP3 OS=Arabidopsis thaliana OX=3702 GN=CEP3 ...	[more]
Q9STL4	2.2e-92	49.57	KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana OX=3702 GN=CEP2 ...	[more]

BLAST of CsGy4G003230 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031740474.1	2.48e-258	99.71	ervatamin-B [Cucumis sativus] >KAE8649110.1 hypothetical protein Csa_014565 [Cuc...	[more]
XP_031740503.1	7.22e-245	94.24	ervatamin-B [Cucumis sativus]	[more]
XP_031739597.1	1.57e-244	93.70	ervatamin-B-like [Cucumis sativus] >KAE8649107.1 hypothetical protein Csa_014529...	[more]
KAE8649111.1	2.38e-233	91.43	hypothetical protein Csa_014425 [Cucumis sativus]	[more]
XP_008454483.1	1.12e-214	82.37	PREDICTED: ervatamin-B-like [Cucumis melo]	[more]

BLAST of CsGy4G003230 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BYQ3	5.40e-215	82.37	ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103494879 PE=3 SV=1	[more]
A0A5A7TM64	1.09e-214	82.37	Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G0034...	[more]
A0A1S3BYU0	2.88e-212	81.27	ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103494878 PE=3 SV=1	[more]
A0A0A0KGB1	7.14e-207	77.49	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G490180 PE=3 SV=1	[more]
A0A5D3D043	6.34e-205	79.37	Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001...	[more]

BLAST of CsGy4G003230 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G48350.1	7.1e-94	49.13	Cysteine proteinases superfamily protein	[more]
AT3G48340.1	1.6e-93	49.57	Cysteine proteinases superfamily protein	[more]
AT5G50260.1	7.3e-91	47.83	Cysteine proteinases superfamily protein	[more]
AT1G20850.1	7.1e-78	44.97	xylem cysteine peptidase 2	[more]
AT5G45890.1	2.5e-75	41.33	senescence-associated gene 12	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000668	Peptidase C1A, papain C-terminal	PRINTS	PR00705	PAPAIN	coord: 302..308 score: 55.51 coord: 144..159 score: 62.45 coord: 287..297 score: 52.4
IPR000668	Peptidase C1A, papain C-terminal	SMART	SM00645	pept_c1	coord: 126..342 e-value: 4.5E-97 score: 338.5
IPR000668	Peptidase C1A, papain C-terminal	PFAM	PF00112	Peptidase_C1	coord: 126..341 e-value: 2.7E-71 score: 240.1
IPR013201	Cathepsin propeptide inhibitor domain (I29)	SMART	SM00848	Inhibitor_I29_2	coord: 41..96 e-value: 1.2E-8 score: 44.7
IPR013201	Cathepsin propeptide inhibitor domain (I29)	PFAM	PF08246	Inhibitor_I29	coord: 41..96 e-value: 8.6E-10 score: 38.9
None	No IPR available	GENE3D	3.90.70.10	Cysteine proteinases	coord: 14..344 e-value: 5.3E-105 score: 353.7
None	No IPR available	PANTHER	PTHR12411:SF706	KDEL-TAILED CYSTEINE ENDOPEPTIDASE CEP1	coord: 21..343
None	No IPR available	PANTHER	PTHR12411	CYSTEINE PROTEASE FAMILY C1-RELATED	coord: 21..343
IPR000169	Cysteine peptidase, cysteine active site	PROSITE	PS00139	THIOL_PROTEASE_CYS	coord: 144..155
IPR039417	Papain-like cysteine endopeptidase	CDD	cd02248	Peptidase_C1A	coord: 127..341 e-value: 7.36636E-96 score: 281.435
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 34..342

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsGy4G003230.2	CsGy4G003230.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0018108	peptidyl-tyrosine phosphorylation
biological_process	GO:0051603	proteolysis involved in cellular protein catabolic process
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005615	extracellular space
cellular_component	GO:0005764	lysosome
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004197	cysteine-type endopeptidase activity
molecular_function	GO:0004713	protein tyrosine kinase activity
molecular_function	GO:0008234	cysteine-type peptidase activity