CsGy4G002810 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy4G002810
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Descriptiondelta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal
LocationGy14Chr4: 1812731 .. 1814211 (-)
RNA-Seq ExpressionCsGy4G002810
SyntenyCsGy4G002810
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTAAACTTTCTACTTTCTGAAACAAAAAAAAGATTATACAAGTAGAAATGTATGAAAATCTAAAACTTAACACACAAGTTCACTTCTCAAAGTATACACTTTCCAATTCTCCAAAATTATAACCCACCAATTTCCGCCGAAGGCCAATGGAGGACTACAAATGCATAAAAATCGAGCGACAAAACCCAAACTCCGGAGTCTTCCATCTCCGCCTCCATCGTCCATCACACTACAACGCTCTCACAACCCAACTCTTCACCGAACTTCGTCAAGCCTTCTCACATCTAGACCAAAACCCCGATGTCCATGTCATCATCCTATCTGGATCCGGCAAACACTTCTGCGCCGGAATCGACCTCAAATCCACAGCCTCTAACTTCGAGAAGCATCTATCGGAGGAACGGGGACGAGCCGGGGAAAGAATCCGACGGGAAATCAAGTGGATGCAAGAGTCAATCACGGCGATTGAGGAGTGCCGGAAGCCGGTGATAGCAAGCATCCATGGAGGGTGTGTTGGAGGGGGAATTGATATAGTGACGGCATGTGATTTGAGGTATTGCACGGCGGAGGCGGTGTTTTCGGTGAGGGAGGTTAAATTGGCGATTACGGCGGATCTTGGGACTTTACAGAGGCTGCCGAGAATCGTAGGGTATGGGAAGGCGGCGGAGTTGGCGTTGACGGGCCGGAATTTTTCGGGCTTGGAGGCTAAGGAATTGGGCTTGGTTTCTCGAACTTTTGATTCTATATCGAAGCTTCAAGATGGAGTCCTCAAAATCGCTGAAGGTAATGAAATTCTCTATACTAACCAATTTTCCTATGCCTTCTCTTTTAGTACCACTCTCATTTCTTACTAGTTTAATATCTTCTGATTAATCTAGTTAAATTAGTGGTTAAAGCATATGATAGATTGTTGTGTAATGTGTATGAGATTGGGAAAAAGGAATGAAAAGTGGATGGTGAATGCAGAAATAGCATCATCATCTCCATTGGCTGTGGTGGGAACAAAGGCAGTGCTACTAAAAAGCAGAGACCTAAATGTAGAACAAGGGTTAGATTATGTTGCAACTTGGAACTCAGGGACTCTACTCTCCGATGATCTCAAAGAAGCCATCTCCGCTCAAGCTAACAAACGCAAACCCAAGTTTTCCAAGCTTTAAGATGGAGAGAGACGTGAACACTTTATCTCTTCATCAAGAAACTCAAGTTACGCCTCTTGCTCTCAAAGCAGCTACATCCTACATTTTATTCTTGATTTCTTGTTCTCAATAATGACATGAAATACTATCGTGTATGTGCCTTTTCTTTTCTCAATCACCTTTGGTTTTTCTTCTTCTTAATAAAAACCAAAAAGTAAAATTGAATATTTCAAGATCCATACTATGTACATTCATTACATAGGACGATAAACGATGCAAGATCACATCGATTGTATTTATCATTCATTACATGGGACGATAAACGATGCAAGATCACATCAATT

mRNA sequence

CCTAAACTTTCTACTTTCTGAAACAAAAAAAAGATTATACAAGTAGAAATGTATGAAAATCTAAAACTTAACACACAAGTTCACTTCTCAAAGTATACACTTTCCAATTCTCCAAAATTATAACCCACCAATTTCCGCCGAAGGCCAATGGAGGACTACAAATGCATAAAAATCGAGCGACAAAACCCAAACTCCGGAGTCTTCCATCTCCGCCTCCATCGTCCATCACACTACAACGCTCTCACAACCCAACTCTTCACCGAACTTCGTCAAGCCTTCTCACATCTAGACCAAAACCCCGATGTCCATGTCATCATCCTATCTGGATCCGGCAAACACTTCTGCGCCGGAATCGACCTCAAATCCACAGCCTCTAACTTCGAGAAGCATCTATCGGAGGAACGGGGACGAGCCGGGGAAAGAATCCGACGGGAAATCAAGTGGATGCAAGAGTCAATCACGGCGATTGAGGAGTGCCGGAAGCCGGTGATAGCAAGCATCCATGGAGGGTGTGTTGGAGGGGGAATTGATATAGTGACGGCATGTGATTTGAGGTATTGCACGGCGGAGGCGGTGTTTTCGGTGAGGGAGGTTAAATTGGCGATTACGGCGGATCTTGGGACTTTACAGAGGCTGCCGAGAATCGTAGGGTATGGGAAGGCGGCGGAGTTGGCGTTGACGGGCCGGAATTTTTCGGGCTTGGAGGCTAAGGAATTGGGCTTGGTTTCTCGAACTTTTGATTCTATATCGAAGCTTCAAGATGGAGTCCTCAAAATCGCTGAAGAAATAGCATCATCATCTCCATTGGCTGTGGTGGGAACAAAGGCAGTGCTACTAAAAAGCAGAGACCTAAATGTAGAACAAGGGTTAGATTATGTTGCAACTTGGAACTCAGGGACTCTACTCTCCGATGATCTCAAAGAAGCCATCTCCGCTCAAGCTAACAAACGCAAACCCAAGTTTTCCAAGCTTTAAGATGGAGAGAGACGTGAACACTTTATCTCTTCATCAAGAAACTCAAGTTACGCCTCTTGCTCTCAAAGCAGCTACATCCTACATTTTATTCTTGATTTCTTGTTCTCAATAATGACATGAAATACTATCGTGTATGTGCCTTTTCTTTTCTCAATCACCTTTGGTTTTTCTTCTTCTTAATAAAAACCAAAAAGTAAAATTGAATATTTCAAGATCCATACTATGTACATTCATTACATAGGACGATAAACGATGCAAGATCACATCGATTGTATTTATCATTCATTACATGGGACGATAAACGATGCAAGATCACATCAATT

Coding sequence (CDS)

ATGGAGGACTACAAATGCATAAAAATCGAGCGACAAAACCCAAACTCCGGAGTCTTCCATCTCCGCCTCCATCGTCCATCACACTACAACGCTCTCACAACCCAACTCTTCACCGAACTTCGTCAAGCCTTCTCACATCTAGACCAAAACCCCGATGTCCATGTCATCATCCTATCTGGATCCGGCAAACACTTCTGCGCCGGAATCGACCTCAAATCCACAGCCTCTAACTTCGAGAAGCATCTATCGGAGGAACGGGGACGAGCCGGGGAAAGAATCCGACGGGAAATCAAGTGGATGCAAGAGTCAATCACGGCGATTGAGGAGTGCCGGAAGCCGGTGATAGCAAGCATCCATGGAGGGTGTGTTGGAGGGGGAATTGATATAGTGACGGCATGTGATTTGAGGTATTGCACGGCGGAGGCGGTGTTTTCGGTGAGGGAGGTTAAATTGGCGATTACGGCGGATCTTGGGACTTTACAGAGGCTGCCGAGAATCGTAGGGTATGGGAAGGCGGCGGAGTTGGCGTTGACGGGCCGGAATTTTTCGGGCTTGGAGGCTAAGGAATTGGGCTTGGTTTCTCGAACTTTTGATTCTATATCGAAGCTTCAAGATGGAGTCCTCAAAATCGCTGAAGAAATAGCATCATCATCTCCATTGGCTGTGGTGGGAACAAAGGCAGTGCTACTAAAAAGCAGAGACCTAAATGTAGAACAAGGGTTAGATTATGTTGCAACTTGGAACTCAGGGACTCTACTCTCCGATGATCTCAAAGAAGCCATCTCCGCTCAAGCTAACAAACGCAAACCCAAGTTTTCCAAGCTTTAA

Protein sequence

MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSGSGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHGGCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGRNFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQGLDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL*
Homology
BLAST of CsGy4G002810 vs. ExPASy Swiss-Prot
Match: Q9FHR8 (Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=DCI1 PE=1 SV=1)

HSP 1 Score: 357.5 bits (916), Expect = 1.4e-97
Identity = 178/276 (64.49%), Postives = 220/276 (79.71%), Query Frame = 0

Query: 1   MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           ME YK ++I R+N +S VFHL ++RPSH NAL+   F E  +A S LDQNPDV VIILSG
Sbjct: 3   MESYKTLEIIRKNTDSSVFHLIINRPSHLNALSLDFFIEFPKALSSLDQNPDVSVIILSG 62

Query: 61  SGKHFCAGIDLKSTAS-NFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIH 120
           +GKHFC+GIDL S +S + +     +RGR+ E++RR+IK MQ +ITAIE+CRKPVIA+IH
Sbjct: 63  AGKHFCSGIDLNSLSSISTQSSSGNDRGRSSEQLRRKIKSMQAAITAIEQCRKPVIAAIH 122

Query: 121 GGCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTG 180
           G C+GGG+D++TACD+RYC+ +A FS++EV LAI ADLGTLQRLP IVGY  A ELALT 
Sbjct: 123 GACIGGGVDLITACDIRYCSEDAFFSIKEVDLAIVADLGTLQRLPSIVGYANAMELALTA 182

Query: 181 RNFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQ 240
           R FSG EAK+LGLVS+ F S S+L +GV  IAE I   SPLAV GTKAVLL+SR+++VEQ
Sbjct: 183 RRFSGSEAKDLGLVSKVFGSKSELDNGVTTIAEGIGGKSPLAVTGTKAVLLRSREVSVEQ 242

Query: 241 GLDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 276
           GLDYVATWNS  L+SDDL EA+SAQ  KRKP+F+KL
Sbjct: 243 GLDYVATWNSAMLISDDLNEAVSAQMMKRKPRFAKL 278

BLAST of CsGy4G002810 vs. ExPASy Swiss-Prot
Match: O35459 (Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Mus musculus OX=10090 GN=Ech1 PE=1 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 8.6e-55
Identity = 110/279 (39.43%), Postives = 169/279 (60.57%), Query Frame = 0

Query: 1   MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           + D+    I+  +    V H++L+RP   NA+    + EL + F  + ++ D   +++SG
Sbjct: 49  VSDHNYESIQVTSAQKHVLHVQLNRPEKRNAMNRAFWRELVECFQKISKDSDCRAVVVSG 108

Query: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120
           +GK F +GIDL   AS   +   ++  R    +R  I   Q++ T IE+C KPVIA+IHG
Sbjct: 109 AGKMFTSGIDLMDMASELMQPSGDDAARIAWYLRDLISKYQKTFTVIEKCPKPVIAAIHG 168

Query: 121 GCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVG-YGKAAELALTG 180
           GC+GGG+D+V+ACD+RYCT +A F ++EV + + AD+GTLQRLP+++G      EL  + 
Sbjct: 169 GCIGGGVDLVSACDIRYCTQDAFFQIKEVDMGLAADVGTLQRLPKVIGNQSLVNELTFSA 228

Query: 181 RNFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQ 240
           R     EA + GLVSR F     + +    +A +I+S SP+AV G+K  L+ SRD +V++
Sbjct: 229 RKMMADEALDSGLVSRVFQDKDAMLNAAFALAADISSKSPVAVQGSKINLIYSRDHSVDE 288

Query: 241 GLDYVATWNSGTLLSDDLKEAISAQANKRKPK---FSKL 276
            LDY+ATWN   L + D+ +++ A   KR  K   FSKL
Sbjct: 289 SLDYMATWNMSMLQTQDIIKSVQAAMEKRDTKSITFSKL 327

BLAST of CsGy4G002810 vs. ExPASy Swiss-Prot
Match: Q62651 (Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Rattus norvegicus OX=10116 GN=Ech1 PE=1 SV=2)

HSP 1 Score: 213.4 bits (542), Expect = 3.3e-54
Identity = 110/279 (39.43%), Postives = 170/279 (60.93%), Query Frame = 0

Query: 1   MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           + D+    I+  +    V H++L+RP   NA+    + EL + F  + ++ D   +++SG
Sbjct: 49  VSDHNYESIQVTSAQKHVLHVQLNRPEKRNAMNRAFWRELVECFQKISKDSDCRAVVVSG 108

Query: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120
           +GK F +GIDL   AS+  +   ++  R    +R  I   Q++ T IE+C KPVIA+IHG
Sbjct: 109 AGKMFTSGIDLMDMASDILQPPGDDVARIAWYLRDLISRYQKTFTVIEKCPKPVIAAIHG 168

Query: 121 GCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAA-ELALTG 180
           GC+GGG+D+++ACD+RYCT +A F V+EV + + AD+GTLQRLP+++G      EL  T 
Sbjct: 169 GCIGGGVDLISACDIRYCTQDAFFQVKEVDVGLAADVGTLQRLPKVIGNRSLVNELTFTA 228

Query: 181 RNFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQ 240
           R     EA + GLVSR F     + +    +A +I+S SP+AV G+K  L+ SRD +V++
Sbjct: 229 RKMMADEALDSGLVSRVFPDKDVMLNAAFALAADISSKSPVAVQGSKINLIYSRDHSVDE 288

Query: 241 GLDYVATWNSGTLLSDDLKEAISAQANKRKPK---FSKL 276
            LDY+ATWN   L + D+ +++ A   K+  K   FSKL
Sbjct: 289 SLDYMATWNMSMLQTQDIIKSVQAAMEKKDSKSITFSKL 327

BLAST of CsGy4G002810 vs. ExPASy Swiss-Prot
Match: Q5RFG0 (Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Pongo abelii OX=9601 GN=ECH1 PE=2 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 5.6e-54
Identity = 111/262 (42.37%), Postives = 163/262 (62.21%), Query Frame = 0

Query: 18  VFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSGSGKHFCAGIDLKSTASN 77
           V H++L+RP+  NA+    + E+ + F+ + ++ D   +++SG+GK F AG+DL   AS+
Sbjct: 67  VLHVQLNRPNKRNAMNKVFWREMVECFNKISRDADCRAVVISGAGKMFTAGVDLMDMASD 126

Query: 78  FEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHGGCVGGGIDIVTACDLRY 137
             +   ++  R    +R  I   QE+   IE+C KPVIA++HGGC+GGG+D+VTACD+RY
Sbjct: 127 ILQPKGDDVARISWYLRDIITRYQETFNVIEKCPKPVIAAVHGGCIGGGVDLVTACDIRY 186

Query: 138 CTAEAVFSVREVKLAITADLGTLQRLPRIVG-YGKAAELALTGRNFSGLEAKELGLVSRT 197
           C  +A F V+EV + + AD+GTLQRLP+++G      ELA T R     EA   GLVSR 
Sbjct: 187 CAQDAFFQVKEVDVGLAADVGTLQRLPKVIGNQSLVNELAFTARKMMADEALGSGLVSRV 246

Query: 198 FDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQGLDYVATWNSGTLLSDD 257
           F     + D  L +A EI+S SP+AV  TK  LL SRD +V + L+YVA+WN   L + D
Sbjct: 247 FPDKEVMLDAALALAAEISSKSPVAVQSTKVNLLYSRDHSVAESLNYVASWNMSMLQTQD 306

Query: 258 LKEAISAQANKRKPK---FSKL 276
           L +++ A    ++ K   FSKL
Sbjct: 307 LMKSVQAATENKELKSVTFSKL 328

BLAST of CsGy4G002810 vs. ExPASy Swiss-Prot
Match: Q13011 (Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Homo sapiens OX=9606 GN=ECH1 PE=1 SV=2)

HSP 1 Score: 212.2 bits (539), Expect = 7.3e-54
Identity = 112/262 (42.75%), Postives = 162/262 (61.83%), Query Frame = 0

Query: 18  VFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSGSGKHFCAGIDLKSTASN 77
           V H++L+RP+  NA+    + E+ + F+ + ++ D   +++SG+GK F AGIDL   AS+
Sbjct: 67  VLHVQLNRPNKRNAMNKVFWREMVECFNKISRDADCRAVVISGAGKMFTAGIDLMDMASD 126

Query: 78  FEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHGGCVGGGIDIVTACDLRY 137
             +   ++  R    +R  I   QE+   IE C KPVIA++HGGC+GGG+D+VTACD+RY
Sbjct: 127 ILQPKGDDVARISWYLRDIITRYQETFNVIERCPKPVIAAVHGGCIGGGVDLVTACDIRY 186

Query: 138 CTAEAVFSVREVKLAITADLGTLQRLPRIVG-YGKAAELALTGRNFSGLEAKELGLVSRT 197
           C  +A F V+EV + + AD+GTLQRLP+++G      ELA T R     EA   GLVSR 
Sbjct: 187 CAQDAFFQVKEVDVGLAADVGTLQRLPKVIGNQSLVNELAFTARKMMADEALGSGLVSRV 246

Query: 198 FDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQGLDYVATWNSGTLLSDD 257
           F     + D  L +A EI+S SP+AV  TK  LL SRD +V + L+YVA+WN   L + D
Sbjct: 247 FPDKEVMLDAALALAAEISSKSPVAVQSTKVNLLYSRDHSVAESLNYVASWNMSMLQTQD 306

Query: 258 LKEAISAQANKRKPK---FSKL 276
           L +++ A    ++ K   FSKL
Sbjct: 307 LVKSVQATTENKELKTVTFSKL 328

BLAST of CsGy4G002810 vs. NCBI nr
Match: XP_004152122.1 (delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal [Cucumis sativus] >KGN53032.1 hypothetical protein Csa_014540 [Cucumis sativus])

HSP 1 Score: 540 bits (1390), Expect = 2.79e-193
Identity = 274/275 (99.64%), Postives = 275/275 (100.00%), Query Frame = 0

Query: 1   MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG
Sbjct: 1   MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60

Query: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120
           SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG
Sbjct: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120

Query: 121 GCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180
           GCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR
Sbjct: 121 GCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180

Query: 181 NFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQG 240
           NFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQG
Sbjct: 181 NFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQG 240

Query: 241 LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 275
           LDYVATWNSGTLLSDDLKEAISAQA+KRKPKFSKL
Sbjct: 241 LDYVATWNSGTLLSDDLKEAISAQASKRKPKFSKL 275

BLAST of CsGy4G002810 vs. NCBI nr
Match: XP_008454026.1 (PREDICTED: delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal [Cucumis melo] >TYK17024.1 delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal [Cucumis melo var. makuwa])

HSP 1 Score: 525 bits (1353), Expect = 1.22e-187
Identity = 266/275 (96.73%), Postives = 272/275 (98.91%), Query Frame = 0

Query: 1   MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           ME Y+CIKIERQ+PNSGVFHLRLHRPSHYNALTTQLFTELRQAFS LDQNPDVHVIILSG
Sbjct: 1   MEGYECIKIERQSPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSRLDQNPDVHVIILSG 60

Query: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120
           SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG
Sbjct: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120

Query: 121 GCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180
           GC+GGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR
Sbjct: 121 GCIGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180

Query: 181 NFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQG 240
           +FSGLEAKELGLVSRTFDS+S+LQDGVLKIAEEIAS SPLAVVGTKAVLLKSRDLNVEQG
Sbjct: 181 DFSGLEAKELGLVSRTFDSMSELQDGVLKIAEEIASRSPLAVVGTKAVLLKSRDLNVEQG 240

Query: 241 LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 275
           LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL
Sbjct: 241 LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 275

BLAST of CsGy4G002810 vs. NCBI nr
Match: KAA0044560.1 (delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal [Cucumis melo var. makuwa])

HSP 1 Score: 522 bits (1345), Expect = 2.02e-186
Identity = 265/275 (96.36%), Postives = 271/275 (98.55%), Query Frame = 0

Query: 1   MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           ME Y+CIKIERQ+PNSGVFHLRLHRPSHYNALTTQLFTELRQAFS LDQNPDVHVIILSG
Sbjct: 1   MEGYECIKIERQSPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSRLDQNPDVHVIILSG 60

Query: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120
           SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG
Sbjct: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120

Query: 121 GCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180
           GC+GGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR
Sbjct: 121 GCIGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180

Query: 181 NFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQG 240
           +FSGLEAKELGLVSRTFDS+S+LQDGVLKIAEEIAS SPLAVVGTKAVLLKSRDL VEQG
Sbjct: 181 DFSGLEAKELGLVSRTFDSMSELQDGVLKIAEEIASRSPLAVVGTKAVLLKSRDLYVEQG 240

Query: 241 LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 275
           LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL
Sbjct: 241 LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 275

BLAST of CsGy4G002810 vs. NCBI nr
Match: XP_038879593.1 (delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal isoform X1 [Benincasa hispida])

HSP 1 Score: 492 bits (1267), Expect = 1.56e-174
Identity = 248/275 (90.18%), Postives = 262/275 (95.27%), Query Frame = 0

Query: 1   MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           ME YKCIKI+RQNPNSGVFHLRLHRPSH+NALTT+LF ELRQAFS+LDQNP+VHVIILSG
Sbjct: 1   MEGYKCIKIDRQNPNSGVFHLRLHRPSHHNALTTELFAELRQAFSYLDQNPEVHVIILSG 60

Query: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120
           SGKHFCAGIDLKSTASNF K  SEERGRAGER+RREIKWMQESITAIEECRKPVIASIHG
Sbjct: 61  SGKHFCAGIDLKSTASNFSKLQSEERGRAGERLRREIKWMQESITAIEECRKPVIASIHG 120

Query: 121 GCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180
           GC+GGG+DIVTACDLRYCTAEAVFSVREV++AITADLGTLQRLPRIVGYGKAAELALTGR
Sbjct: 121 GCIGGGVDIVTACDLRYCTAEAVFSVREVRMAITADLGTLQRLPRIVGYGKAAELALTGR 180

Query: 181 NFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQG 240
           +FSGLEAKELGLVSRTF S  +L+D VLKIA+EI S SPLAVVGTKAVLLKSRDLNVEQG
Sbjct: 181 DFSGLEAKELGLVSRTFASKLELEDEVLKIAQEIGSKSPLAVVGTKAVLLKSRDLNVEQG 240

Query: 241 LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 275
           LDYVATWNSGTLLSDDLKEAISAQANKR P FSKL
Sbjct: 241 LDYVATWNSGTLLSDDLKEAISAQANKRNPVFSKL 275

BLAST of CsGy4G002810 vs. NCBI nr
Match: XP_023526792.1 (delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal [Cucurbita pepo subsp. pepo])

HSP 1 Score: 460 bits (1184), Expect = 6.91e-162
Identity = 235/275 (85.45%), Postives = 250/275 (90.91%), Query Frame = 0

Query: 1   MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           MEDYK IKIERQNP+SGVFHLRLHRPSHYNALTT+LF ELR+A S LDQNPDV VIILSG
Sbjct: 1   MEDYKSIKIERQNPSSGVFHLRLHRPSHYNALTTELFAELRRALSSLDQNPDVRVIILSG 60

Query: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120
           SGKHFCAGIDLKSTASNFE  LS ERGRAGER+RRE+K MQ+SITAIEECRKPVIA IHG
Sbjct: 61  SGKHFCAGIDLKSTASNFENLLSSERGRAGERLRREVKAMQDSITAIEECRKPVIACIHG 120

Query: 121 GCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180
           GC+GGG+DI TACDLRYCTAEAVFSV+EV LAITADLGTLQRLP IVG+GKA ELALTGR
Sbjct: 121 GCIGGGVDIATACDLRYCTAEAVFSVKEVNLAITADLGTLQRLPGIVGFGKAVELALTGR 180

Query: 181 NFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQG 240
            FSGLEAKELGLVS TF S  +L +GV+KIAEEIAS SPLAVVGTKAVLLKSRDL VEQG
Sbjct: 181 EFSGLEAKELGLVSTTFASKMELDNGVIKIAEEIASKSPLAVVGTKAVLLKSRDLTVEQG 240

Query: 241 LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 275
           LDYVATWNSGTLLS DLKEAISA+A+KR P FSKL
Sbjct: 241 LDYVATWNSGTLLSADLKEAISARAHKRNPTFSKL 275

BLAST of CsGy4G002810 vs. ExPASy TrEMBL
Match: A0A0A0KXB8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G011790 PE=3 SV=1)

HSP 1 Score: 540 bits (1390), Expect = 1.35e-193
Identity = 274/275 (99.64%), Postives = 275/275 (100.00%), Query Frame = 0

Query: 1   MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG
Sbjct: 1   MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60

Query: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120
           SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG
Sbjct: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120

Query: 121 GCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180
           GCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR
Sbjct: 121 GCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180

Query: 181 NFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQG 240
           NFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQG
Sbjct: 181 NFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQG 240

Query: 241 LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 275
           LDYVATWNSGTLLSDDLKEAISAQA+KRKPKFSKL
Sbjct: 241 LDYVATWNSGTLLSDDLKEAISAQASKRKPKFSKL 275

BLAST of CsGy4G002810 vs. ExPASy TrEMBL
Match: A0A5D3D1B6 (Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001700 PE=3 SV=1)

HSP 1 Score: 525 bits (1353), Expect = 5.90e-188
Identity = 266/275 (96.73%), Postives = 272/275 (98.91%), Query Frame = 0

Query: 1   MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           ME Y+CIKIERQ+PNSGVFHLRLHRPSHYNALTTQLFTELRQAFS LDQNPDVHVIILSG
Sbjct: 1   MEGYECIKIERQSPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSRLDQNPDVHVIILSG 60

Query: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120
           SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG
Sbjct: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120

Query: 121 GCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180
           GC+GGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR
Sbjct: 121 GCIGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180

Query: 181 NFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQG 240
           +FSGLEAKELGLVSRTFDS+S+LQDGVLKIAEEIAS SPLAVVGTKAVLLKSRDLNVEQG
Sbjct: 181 DFSGLEAKELGLVSRTFDSMSELQDGVLKIAEEIASRSPLAVVGTKAVLLKSRDLNVEQG 240

Query: 241 LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 275
           LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL
Sbjct: 241 LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 275

BLAST of CsGy4G002810 vs. ExPASy TrEMBL
Match: A0A1S3BXS5 (delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103494575 PE=3 SV=1)

HSP 1 Score: 525 bits (1353), Expect = 5.90e-188
Identity = 266/275 (96.73%), Postives = 272/275 (98.91%), Query Frame = 0

Query: 1   MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           ME Y+CIKIERQ+PNSGVFHLRLHRPSHYNALTTQLFTELRQAFS LDQNPDVHVIILSG
Sbjct: 1   MEGYECIKIERQSPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSRLDQNPDVHVIILSG 60

Query: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120
           SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG
Sbjct: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120

Query: 121 GCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180
           GC+GGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR
Sbjct: 121 GCIGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180

Query: 181 NFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQG 240
           +FSGLEAKELGLVSRTFDS+S+LQDGVLKIAEEIAS SPLAVVGTKAVLLKSRDLNVEQG
Sbjct: 181 DFSGLEAKELGLVSRTFDSMSELQDGVLKIAEEIASRSPLAVVGTKAVLLKSRDLNVEQG 240

Query: 241 LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 275
           LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL
Sbjct: 241 LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 275

BLAST of CsGy4G002810 vs. ExPASy TrEMBL
Match: A0A5A7TMZ4 (Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G003090 PE=3 SV=1)

HSP 1 Score: 522 bits (1345), Expect = 9.78e-187
Identity = 265/275 (96.36%), Postives = 271/275 (98.55%), Query Frame = 0

Query: 1   MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           ME Y+CIKIERQ+PNSGVFHLRLHRPSHYNALTTQLFTELRQAFS LDQNPDVHVIILSG
Sbjct: 1   MEGYECIKIERQSPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSRLDQNPDVHVIILSG 60

Query: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120
           SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG
Sbjct: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120

Query: 121 GCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180
           GC+GGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR
Sbjct: 121 GCIGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180

Query: 181 NFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQG 240
           +FSGLEAKELGLVSRTFDS+S+LQDGVLKIAEEIAS SPLAVVGTKAVLLKSRDL VEQG
Sbjct: 181 DFSGLEAKELGLVSRTFDSMSELQDGVLKIAEEIASRSPLAVVGTKAVLLKSRDLYVEQG 240

Query: 241 LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 275
           LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL
Sbjct: 241 LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 275

BLAST of CsGy4G002810 vs. ExPASy TrEMBL
Match: A0A6J1GW60 (delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111458038 PE=3 SV=1)

HSP 1 Score: 460 bits (1183), Expect = 4.75e-162
Identity = 234/275 (85.09%), Postives = 249/275 (90.55%), Query Frame = 0

Query: 1   MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           MEDYK IKIERQNPNSGVFHLRLHRPSHYNALTT+LF ELR+A S LDQNPDV VIILSG
Sbjct: 1   MEDYKSIKIERQNPNSGVFHLRLHRPSHYNALTTELFAELRRALSSLDQNPDVRVIILSG 60

Query: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120
           SGKHFCAGIDLKSTASNFE  LS ERGRAGER+RRE+K MQ+SITAIEECRKPVIA IHG
Sbjct: 61  SGKHFCAGIDLKSTASNFEDLLSSERGRAGERLRREVKAMQDSITAIEECRKPVIACIHG 120

Query: 121 GCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180
           GC+GGG+DI TACDLRYCTAEAVFSV+E+ LAITADLGTLQRLP IVG+GKA ELALTGR
Sbjct: 121 GCIGGGVDIATACDLRYCTAEAVFSVKEINLAITADLGTLQRLPGIVGFGKAVELALTGR 180

Query: 181 NFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQG 240
            FSG EAKELGLVS TF S  +L +GV+KIAEEIAS SPLAVVGTKAVLLKSRDL VEQG
Sbjct: 181 EFSGFEAKELGLVSTTFASKMELDNGVIKIAEEIASKSPLAVVGTKAVLLKSRDLTVEQG 240

Query: 241 LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 275
           LDYVATWNSGTLLS DLKEAISA+A+KR P FSKL
Sbjct: 241 LDYVATWNSGTLLSADLKEAISARAHKRNPTFSKL 275

BLAST of CsGy4G002810 vs. TAIR 10
Match: AT5G43280.1 (delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1 )

HSP 1 Score: 357.5 bits (916), Expect = 1.0e-98
Identity = 178/276 (64.49%), Postives = 220/276 (79.71%), Query Frame = 0

Query: 1   MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           ME YK ++I R+N +S VFHL ++RPSH NAL+   F E  +A S LDQNPDV VIILSG
Sbjct: 3   MESYKTLEIIRKNTDSSVFHLIINRPSHLNALSLDFFIEFPKALSSLDQNPDVSVIILSG 62

Query: 61  SGKHFCAGIDLKSTAS-NFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIH 120
           +GKHFC+GIDL S +S + +     +RGR+ E++RR+IK MQ +ITAIE+CRKPVIA+IH
Sbjct: 63  AGKHFCSGIDLNSLSSISTQSSSGNDRGRSSEQLRRKIKSMQAAITAIEQCRKPVIAAIH 122

Query: 121 GGCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTG 180
           G C+GGG+D++TACD+RYC+ +A FS++EV LAI ADLGTLQRLP IVGY  A ELALT 
Sbjct: 123 GACIGGGVDLITACDIRYCSEDAFFSIKEVDLAIVADLGTLQRLPSIVGYANAMELALTA 182

Query: 181 RNFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQ 240
           R FSG EAK+LGLVS+ F S S+L +GV  IAE I   SPLAV GTKAVLL+SR+++VEQ
Sbjct: 183 RRFSGSEAKDLGLVSKVFGSKSELDNGVTTIAEGIGGKSPLAVTGTKAVLLRSREVSVEQ 242

Query: 241 GLDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 276
           GLDYVATWNS  L+SDDL EA+SAQ  KRKP+F+KL
Sbjct: 243 GLDYVATWNSAMLISDDLNEAVSAQMMKRKPRFAKL 278

BLAST of CsGy4G002810 vs. TAIR 10
Match: AT5G43280.2 (delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1 )

HSP 1 Score: 269.6 bits (688), Expect = 2.7e-72
Identity = 133/214 (62.15%), Postives = 168/214 (78.50%), Query Frame = 0

Query: 1   MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           ME YK ++I R+N +S VFHL ++RPSH NAL+   F E  +A S LDQNPDV VIILSG
Sbjct: 3   MESYKTLEIIRKNTDSSVFHLIINRPSHLNALSLDFFIEFPKALSSLDQNPDVSVIILSG 62

Query: 61  SGKHFCAGIDLKSTAS-NFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIH 120
           +GKHFC+GIDL S +S + +     +RGR+ E++RR+IK MQ +ITAIE+CRKPVIA+IH
Sbjct: 63  AGKHFCSGIDLNSLSSISTQSSSGNDRGRSSEQLRRKIKSMQAAITAIEQCRKPVIAAIH 122

Query: 121 GGCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTG 180
           G C+GGG+D++TACD+RYC+ +A FS++EV LAI ADLGTLQRLP IVGY  A ELALT 
Sbjct: 123 GACIGGGVDLITACDIRYCSEDAFFSIKEVDLAIVADLGTLQRLPSIVGYANAMELALTA 182

Query: 181 RNFSGLEAKELGLVSRTFDSISKLQDGVLKIAEE 214
           R FSG EAK+LGLVS+ F S S+L +GV  IAE+
Sbjct: 183 RRFSGSEAKDLGLVSKVFGSKSELDNGVTTIAED 216

BLAST of CsGy4G002810 vs. TAIR 10
Match: AT4G16800.1 (ATP-dependent caseinolytic (Clp) protease/crotonase family protein )

HSP 1 Score: 124.8 bits (312), Expect = 1.1e-28
Identity = 82/269 (30.48%), Postives = 131/269 (48.70%), Query Frame = 0

Query: 7   IKIER-QNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIIL-SGSGKH 66
           +K+ R    +SG+  + L RP   NA+  ++   L+ AF  + Q+    V+++ S     
Sbjct: 43  VKLNRLSGSDSGIIEVNLDRPVTKNAINKEMLKSLQNAFESIHQDNSARVVMIRSLVPGV 102

Query: 67  FCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHGGCVG 126
           FCAG DLK           E R  +   +   +  ++   + IE    P IA+I G  +G
Sbjct: 103 FCAGADLK-----------ERRTMSPSEVHTYVNSLRYMFSFIEALSIPTIAAIEGAALG 162

Query: 127 GGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGRNFSG 186
           GG+++  ACDLR C   AVF + E  LAI    G  QRL R+VG   + EL  TGR    
Sbjct: 163 GGLEMALACDLRICGENAVFGLPETGLAIIPGAGGTQRLSRLVGRSVSKELIFTGRKIDA 222

Query: 187 LEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQGLDYV 246
           +EA   GLV+    +  +  +  +++A++I    PLA+   K  + +  + N+  GL+  
Sbjct: 223 IEAANKGLVNICV-TAGEAHEKAIEMAQQINEKGPLAIKMAKKAIDEGIETNMASGLEVE 282

Query: 247 ATWNSGTLLSDDLKEAISAQANKRKPKFS 274
                  L + D  E ++A A KRKP ++
Sbjct: 283 EMCYQKLLNTQDRLEGLAAFAEKRKPLYT 299

BLAST of CsGy4G002810 vs. TAIR 10
Match: AT4G16210.1 (enoyl-CoA hydratase/isomerase A )

HSP 1 Score: 102.8 bits (255), Expect = 4.4e-22
Identity = 63/233 (27.04%), Postives = 113/233 (48.50%), Query Frame = 0

Query: 9   IERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSGSGKHFCAG 68
           I+ +  + G+  + ++RP   N+LT  +  +L +AF  +D +  V V+I +GSG+ FC+G
Sbjct: 10  IQVKKESGGIAVITINRPKSLNSLTRAMMVDLAKAFKDMDSDESVQVVIFTGSGRSFCSG 69

Query: 69  IDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHGGCVGGGID 128
           +DL +  S F+  + +                 + +  +E  RKP+I +I+G  +  G +
Sbjct: 70  VDLTAAESVFKGDVKDPE--------------TDPVVQMERLRKPIIGAINGFAITAGFE 129

Query: 129 IVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGRNFSGLEAK 188
           +  ACD+   +  A F     +  I    G  Q+L RI+G  KA E++LT    +   A 
Sbjct: 130 LALACDILVASRGAKFMDTHARFGIFPSWGLSQKLSRIIGANKAREVSLTSMPLTADVAG 189

Query: 189 ELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQGL 242
           +LG V+   +    L+    +IAE I  +    V+  K+V+     L++   L
Sbjct: 190 KLGFVNHVVEEGEALKK-AREIAEAIIKNEQGMVLRIKSVINDGLKLDLGHAL 227

BLAST of CsGy4G002810 vs. TAIR 10
Match: AT4G29010.1 (Enoyl-CoA hydratase/isomerase family )

HSP 1 Score: 89.4 bits (220), Expect = 5.1e-18
Identity = 58/211 (27.49%), Postives = 102/211 (48.34%), Query Frame = 0

Query: 5   KCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSGSGKH 64
           K I +  +  N GV  + +  P   N+L + + + L++ F   +Q  DV  I+L G+   
Sbjct: 3   KKIGVTMEVGNDGVAVITISNPP-VNSLASPIISGLKEKFRDANQRNDVKAIVLIGNNGR 62

Query: 65  FCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHGGCVG 124
           F  G D+         ++ ++  + G+        ++     +E+ RKPV+A++ G  +G
Sbjct: 63  FSGGFDI---------NVFQQVHKTGDLSLMPEVSVELVCNLMEDSRKPVVAAVEGLALG 122

Query: 125 GGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGRNFSG 184
           GG+++  AC  R    +A   + E+ L +    G  QRLPR+VG  KA ++ L  ++ S 
Sbjct: 123 GGLELAMACHARVAAPKAQLGLPELTLGVIPGFGGTQRLPRLVGLAKATDMILLSKSISS 182

Query: 185 LEAKELGLVSRTF---DSISKLQDGVLKIAE 213
            E  +LGL+       D +S  +   L IAE
Sbjct: 183 EEGHKLGLIDALVPPGDVLSTSRKWALDIAE 203

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FHR81.4e-9764.49Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Arabidopsis thaliana... [more]
O354598.6e-5539.43Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Mus musculus OX=10... [more]
Q626513.3e-5439.43Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Rattus norvegicus ... [more]
Q5RFG05.6e-5442.37Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Pongo abelii OX=96... [more]
Q130117.3e-5442.75Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Homo sapiens OX=96... [more]
Match NameE-valueIdentityDescription
XP_004152122.12.79e-19399.64delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal [Cucumis sativus] >KGN5... [more]
XP_008454026.11.22e-18796.73PREDICTED: delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal [Cucumis mel... [more]
KAA0044560.12.02e-18696.36delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal [Cucumis melo var. maku... [more]
XP_038879593.11.56e-17490.18delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal isoform X1 [Benincasa h... [more]
XP_023526792.16.91e-16285.45delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal [Cucurbita pepo subsp. ... [more]
Match NameE-valueIdentityDescription
A0A0A0KXB81.35e-19399.64Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G011790 PE=3 SV=1[more]
A0A5D3D1B65.90e-18896.73Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Cucumis melo var. ma... [more]
A0A1S3BXS55.90e-18896.73delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Cucumis melo OX=3656... [more]
A0A5A7TMZ49.78e-18796.36Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Cucumis melo var. ma... [more]
A0A6J1GW604.75e-16285.09delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Cucurbita moschata O... [more]
Match NameE-valueIdentityDescription
AT5G43280.11.0e-9864.49delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1 [more]
AT5G43280.22.7e-7262.15delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1 [more]
AT4G16800.11.1e-2830.48ATP-dependent caseinolytic (Clp) protease/crotonase family protein [more]
AT4G16210.14.4e-2227.04enoyl-CoA hydratase/isomerase A [more]
AT4G29010.15.1e-1827.49Enoyl-CoA hydratase/isomerase family [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014748Enoyl-CoA hydratase, C-terminalGENE3D1.10.12.10coord: 218..275
e-value: 3.1E-24
score: 86.9
IPR001753Enoyl-CoA hydratase/isomerasePFAMPF00378ECH_1coord: 15..273
e-value: 1.6E-51
score: 175.0
NoneNo IPR availableGENE3D3.90.226.10coord: 1..217
e-value: 7.5E-65
score: 220.4
NoneNo IPR availableCDDcd06558crotonase-likecoord: 7..214
e-value: 2.54149E-71
score: 215.887
IPR045002Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase Ech1-likePANTHERPTHR43149ENOYL-COA HYDRATASEcoord: 2..275
IPR018376Enoyl-CoA hydratase/isomerase, conserved sitePROSITEPS00166ENOYL_COA_HYDRATASEcoord: 115..135
IPR029045ClpP/crotonase-like domain superfamilySUPERFAMILY52096ClpP/crotonasecoord: 3..273

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy4G002810.2CsGy4G002810.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0044281 small molecule metabolic process
cellular_component GO:0005777 peroxisome
molecular_function GO:0051750 delta3,5-delta2,4-dienoyl-CoA isomerase activity
molecular_function GO:0003824 catalytic activity