CsGy4G000817 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy4G000817
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionEpoxide hydrolase 2
LocationGy14Chr4: 501220 .. 502580 (-)
RNA-Seq ExpressionCsGy4G000817
SyntenyCsGy4G000817
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GACTGAATTAGGTAAGGCTCCTGCATGCAAAATTGCTTCCATTATTTAAACCCACATAGAGAATAAACAAAACAAAGCCATCAATGGAGAAAATCCAACACTCAACCATCCCTACCAATGGCATTAACATCCACCTTGCTTCCATTGGCTCCGGCCCCGCGTTGCTATTCCTCCATGGCTTCCCAGAGATCTGGTACACATTGCGTCACCAGCTTCTCTTTTTTGCTTCCAGGGGCTTCCGAGCCATAGCGCCTGATCTCCGTGGATATGGTGACTCCGATGTGCCACCATCTCCTTCTTCCTATACAGCTCACCACATCGTCGGCGATCTCATTGGCCTCCTCGACCATCTCGAAATCGATCAAGTTTTCTTAGTAGGCCACGACTTGGGAGCTACTATAGCTTGGTACTTTTCCATTTTCAGGCCTGATCGAGTCAAGGCTTTGGTCAATTTGAGTGTTTATCATACTCCTCGGATTCCGGAAATGCCGCCTCTCGCCATTATCCGTTTGTTCCTTGGTGATGATTTCTACATTTGTAAATTTCAGGTGAAGGCGATTGAGTCTTTGAACTCTAGCACAAATTCAATTGGGATACATATATCTGATGAGTTTCAATCACATGCATTAATTAAATGTACTGGTTAATAATGAACAGAAAATTGTGAATTTTGAAACAGGAACCTGGAGTCGCAGAAGCAGATTTAGGGTCAGTAGATACGGCTACGATGATGAAGAAATTCCTGACGTTGAGAGATCCAAGCGCTCCGATTGCACCAAACGGATTCAGTACACTACTGGCAACTCCAGAAACCTTACCTTCGTGGTTAACAGAGGATGATGTAGATTATTACGCTTCCAAATTCGCGAAAACCGGCTTCACCGGCGGACTAAACTACTATCGTGCTCTTGACCTGTGTGGATAGTAACATATTATATTCTTCGTATTTTGAATCAATTGGATTATCTAATTAGAAACCGATGTCGTTTGTGTGCGTGTGTTTTGTTGATTGCTTGTAGAACTTGGGAGCTGACGGGGCCATGGACTGGAGCAAAGATCAAAGTTCCAACAAAATTCATTGTTGGGGATCAAGATCTGGTATACCATTTTCCGGGGGCGAAGGAGTACATTCACGGCGACAGTTTCAAGGAGGACGTACCGCATTTAGAAGAAGTGGTGGTGATAGAAGGAGCAGCTCACTTCATTAACCAAGAGAAGGCCGATGAAATAAATTCCCTCATCTACGATTTCATCACCAAATTCTAAATAATTCTCACATGGCACACCCCACTACTACTACTGCACTTACCTCATATGGTCTTATATTTTAGCACGTTTACATTTTTAATATTACTTCGTGA

mRNA sequence

GACTGAATTAGGTAAGGCTCCTGCATGCAAAATTGCTTCCATTATTTAAACCCACATAGAGAATAAACAAAACAAAGCCATCAATGGAGAAAATCCAACACTCAACCATCCCTACCAATGGCATTAACATCCACCTTGCTTCCATTGGCTCCGGCCCCGCGTTGCTATTCCTCCATGGCTTCCCAGAGATCTGGTACACATTGCGTCACCAGCTTCTCTTTTTTGCTTCCAGGGGCTTCCGAGCCATAGCGCCTGATCTCCGTGGATATGGTGACTCCGATGTGCCACCATCTCCTTCTTCCTATACAGCTCACCACATCGTCGGCGATCTCATTGGCCTCCTCGACCATCTCGAAATCGATCAAGTTTTCTTAGTAGGCCACGACTTGGGAGCTACTATAGCTTGGTACTTTTCCATTTTCAGGCCTGATCGAGTCAAGGCTTTGGTCAATTTGAGTGTTTATCATACTCCTCGGATTCCGGAAATGCCGCCTCTCGCCATTATCCGTTTGTTCCTTGGTGATGATTTCTACATTTGTAAATTTCAGGAACCTGGAGTCGCAGAAGCAGATTTAGGGTCAGTAGATACGGCTACGATGATGAAGAAATTCCTGACGTTGAGAGATCCAAGCGCTCCGATTGCACCAAACGGATTCAGTACACTACTGGCAACTCCAGAAACCTTACCTTCGTGGTTAACAGAGGATGATGTAGATTATTACGCTTCCAAATTCGCGAAAACCGGCTTCACCGGCGGACTAAACTACTATCGTGCTCTTGACCTAACTTGGGAGCTGACGGGGCCATGGACTGGAGCAAAGATCAAAGTTCCAACAAAATTCATTGTTGGGGATCAAGATCTGGTATACCATTTTCCGGGGGCGAAGGAGTACATTCACGGCGACAGTTTCAAGGAGGACGTACCGCATTTAGAAGAAGTGGTGGTGATAGAAGGAGCAGCTCACTTCATTAACCAAGAGAAGGCCGATGAAATAAATTCCCTCATCTACGATTTCATCACCAAATTCTAAATAATTCTCACATGGCACACCCCACTACTACTACTGCACTTACCTCATATGGTCTTATATTTTAGCACGTTTACATTTTTAATATTACTTCGTGA

Coding sequence (CDS)

ATGGAGAAAATCCAACACTCAACCATCCCTACCAATGGCATTAACATCCACCTTGCTTCCATTGGCTCCGGCCCCGCGTTGCTATTCCTCCATGGCTTCCCAGAGATCTGGTACACATTGCGTCACCAGCTTCTCTTTTTTGCTTCCAGGGGCTTCCGAGCCATAGCGCCTGATCTCCGTGGATATGGTGACTCCGATGTGCCACCATCTCCTTCTTCCTATACAGCTCACCACATCGTCGGCGATCTCATTGGCCTCCTCGACCATCTCGAAATCGATCAAGTTTTCTTAGTAGGCCACGACTTGGGAGCTACTATAGCTTGGTACTTTTCCATTTTCAGGCCTGATCGAGTCAAGGCTTTGGTCAATTTGAGTGTTTATCATACTCCTCGGATTCCGGAAATGCCGCCTCTCGCCATTATCCGTTTGTTCCTTGGTGATGATTTCTACATTTGTAAATTTCAGGAACCTGGAGTCGCAGAAGCAGATTTAGGGTCAGTAGATACGGCTACGATGATGAAGAAATTCCTGACGTTGAGAGATCCAAGCGCTCCGATTGCACCAAACGGATTCAGTACACTACTGGCAACTCCAGAAACCTTACCTTCGTGGTTAACAGAGGATGATGTAGATTATTACGCTTCCAAATTCGCGAAAACCGGCTTCACCGGCGGACTAAACTACTATCGTGCTCTTGACCTAACTTGGGAGCTGACGGGGCCATGGACTGGAGCAAAGATCAAAGTTCCAACAAAATTCATTGTTGGGGATCAAGATCTGGTATACCATTTTCCGGGGGCGAAGGAGTACATTCACGGCGACAGTTTCAAGGAGGACGTACCGCATTTAGAAGAAGTGGTGGTGATAGAAGGAGCAGCTCACTTCATTAACCAAGAGAAGGCCGATGAAATAAATTCCCTCATCTACGATTTCATCACCAAATTCTAA

Protein sequence

MEKIQHSTIPTNGINIHLASIGSGPALLFLHGFPEIWYTLRHQLLFFASRGFRAIAPDLRGYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKALVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLRDPSAPIAPNGFSTLLATPETLPSWLTEDDVDYYASKFAKTGFTGGLNYYRALDLTWELTGPWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPHLEEVVVIEGAAHFINQEKADEINSLIYDFITKF*
Homology
BLAST of CsGy4G000817 vs. ExPASy Swiss-Prot
Match: I6YGS0 (Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=ephA PE=1 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 7.3e-58
Identity = 121/319 (37.93%), Postives = 174/319 (54.55%), Query Frame = 0

Query: 9   IPTNGINIHLASIG--SGPALLFLHGFPEIWYTLRHQLLFFASRGFRAIAPDLRGYGDSD 68
           + TNG+ + +   G    P ++  HGFPE+ Y+ RHQ+   A  G+  +APD RGYG S 
Sbjct: 9   VDTNGVRLRVVEAGEPGAPVVILAHGFPELAYSWRHQIPALADAGYHVLAPDQRGYGGSS 68

Query: 69  VPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKALVNLSV 128
            P +  +Y  H +  DL+GLLD +  ++   VGHD GA + W   +   DRV A+  LSV
Sbjct: 69  RPEAIEAYDIHRLTADLVGLLDDVGAERAVWVGHDWGAVVVWNAPLLHADRVAAVAALSV 128

Query: 129 YHTPRIPEMPPLAIIRLFLGDD-FYICKFQEPGVAEADLGSVDTATMMKKFLTLRDPSAP 188
              PR  ++PP    R   G++ FYI  FQEPG+A+A+L      TM +    LR P   
Sbjct: 129 PALPR-AQVPPTQAFRSRFGENFFYILYFQEPGIADAELNGDPARTMRRMIGGLRPPGDQ 188

Query: 189 IA--------PNGFSTLLATPETLPSWLTEDDVDYYASKFAKTGFTGGLNYYRALDLTWE 248
            A        P+GF   L  P  LP+W++++++D+Y  +F +TGFTGGLN+YR  D  WE
Sbjct: 189 SAAMRMLAPGPDGFIDRLPEPAGLPAWISQEELDHYIGEFTRTGFTGGLNWYRNFDRNWE 248

Query: 249 LTGPWTGAKIKVPTKFIVGDQDLVYHF---PGAKEYIHGDSFKEDVPHLEEVVVIEGAAH 308
            T    G  I VP+ FI G  D V  F     A E I G       P+ E  V+I+GA H
Sbjct: 249 TTADLAGKTISVPSLFIAGTADPVLTFTRTDRAAEVISG-------PYRE--VLIDGAGH 308

Query: 309 FINQEKADEINSLIYDFIT 314
           ++ QE+  E+ + + +F+T
Sbjct: 309 WLQQERPGEVTAALLEFLT 317

BLAST of CsGy4G000817 vs. ExPASy Swiss-Prot
Match: Q6Q2C2 (Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 2.9e-46
Identity = 100/306 (32.68%), Postives = 167/306 (54.58%), Query Frame = 0

Query: 13  GINIHLASIGSGPALLFLHGFPEIWYTLRHQLLFFASRGFRAIAPDLRGYGDSDVPPSPS 72
           G+ +H   +GSGPA+   HGFPE W++ R+Q+   A  GFR +A D++GYG+S  PP   
Sbjct: 247 GVRLHFVEMGSGPAVCLCHGFPESWFSWRYQIPALAQAGFRVLAVDMKGYGESSAPPEIE 306

Query: 73  SYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKALVNLSVYHTPRI 132
            Y+   +  D++  L+ L + Q   +GHD G  + W  ++F P+RV+A+ +L+    P  
Sbjct: 307 EYSLEVLCKDMVTFLNKLGLSQAVFIGHDWGGVLVWNMALFYPERVRAVASLNTPFMPSN 366

Query: 133 PEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLRDPSAPIAPNGFS 192
           P + P+ II+     D+ +  FQEPGVAEA+L   +     K F    D +  +  N   
Sbjct: 367 PNVSPMEIIKANPVFDYQL-YFQEPGVAEAEL-EQNLDRTFKNFFRAHDETF-LTTNRVR 426

Query: 193 TL----LATPE--TLPSWLTEDDVDYYASKFAKTGFTGGLNYYRALDLTWELTGPWTGAK 252
            L    + TPE  +L   +TE+D+ +Y  +F K+GF G LN+YR ++  W+     +G K
Sbjct: 427 ELGGLFVGTPEEPSLSRLVTEEDIQFYVQQFKKSGFRGPLNWYRNMERNWQWGCKGSGRK 486

Query: 253 IKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPHLEEVVVIEGAAHFINQEKADEINS 312
           I +P   +  + DLV H   +K   H +++   +PHL+    I+   H+   +K  E+N 
Sbjct: 487 ILIPALMVTAENDLVLHPKMSK---HMENW---IPHLKR-GHIKDCGHWTQIDKPAELNR 542

BLAST of CsGy4G000817 vs. ExPASy Swiss-Prot
Match: P34914 (Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2)

HSP 1 Score: 185.7 bits (470), Expect = 8.4e-46
Identity = 102/306 (33.33%), Postives = 164/306 (53.59%), Query Frame = 0

Query: 13  GINIHLASIGSGPALLFLHGFPEIWYTLRHQLLFFASRGFRAIAPDLRGYGDSDVPPSPS 72
           GI +H   +GSGPAL   HGFPE W++ R+Q+   A  GFR +A D++GYGDS  PP   
Sbjct: 245 GIRLHFVEMGSGPALCLCHGFPESWFSWRYQIPALAQAGFRVLAIDMKGYGDSSSPPEIE 304

Query: 73  SYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKALVNLSVYHTPRI 132
            Y    +  +++  LD L I Q   +GHD    + W  ++F P+RV+A+ +L+    P  
Sbjct: 305 EYAMELLCKEMVTFLDKLGIPQAVFIGHDWAGVMVWNMALFYPERVRAVASLNTPFMPPD 364

Query: 133 PEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLRDPSAPIAPNGFS 192
           P++ P+ +IR     ++ +  FQEPGVAEA+L   + +   K F    D +  IA +  +
Sbjct: 365 PDVSPMKVIRSIPVFNYQL-YFQEPGVAEAEL-EKNMSRTFKSFFRASDETGFIAVHKAT 424

Query: 193 ----TLLATPE--TLPSWLTEDDVDYYASKFAKTGFTGGLNYYRALDLTWELTGPWTGAK 252
                L+ TPE   L    TE+++++Y  +F KTGF G LN+YR  +  W+ +    G K
Sbjct: 425 EIGGILVNTPEDPNLSKITTEEEIEFYIQQFKKTGFRGPLNWYRNTERNWKWSCKGLGRK 484

Query: 253 IKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPHLEEVVVIEGAAHFINQEKADEINS 312
           I VP   +  ++D+V     +K      + ++ +P L+    IE   H+   EK  E+N 
Sbjct: 485 ILVPALMVTAEKDIVLRPEMSK------NMEKWIPFLKR-GHIEDCGHWTQIEKPTEVNQ 541

BLAST of CsGy4G000817 vs. ExPASy Swiss-Prot
Match: P34913 (Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2)

HSP 1 Score: 181.8 bits (460), Expect = 1.2e-44
Identity = 96/305 (31.48%), Postives = 161/305 (52.79%), Query Frame = 0

Query: 14  INIHLASIGSGPALLFLHGFPEIWYTLRHQLLFFASRGFRAIAPDLRGYGDSDVPPSPSS 73
           + +H   +GSGPA+   HGFPE WY+ R+Q+   A  G+R +A D++GYG+S  PP    
Sbjct: 248 VRLHFVELGSGPAVCLCHGFPESWYSWRYQIPALAQAGYRVLAMDMKGYGESSAPPEIEE 307

Query: 74  YTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKALVNLSVYHTPRIP 133
           Y    +  +++  LD L + Q   +GHD G  + WY ++F P+RV+A+ +L+    P  P
Sbjct: 308 YCMEVLCKEMVTFLDKLGLSQAVFIGHDWGGMLVWYMALFYPERVRAVASLNTPFIPANP 367

Query: 134 EMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLRDPSA----PIAPN 193
            M PL  I+     D+ +  FQEPGVAEA+L   + +   K      D S      +   
Sbjct: 368 NMSPLESIKANPVFDYQL-YFQEPGVAEAEL-EQNLSRTFKSLFRASDESVLSMHKVCEA 427

Query: 194 GFSTLLATPE--TLPSWLTEDDVDYYASKFAKTGFTGGLNYYRALDLTWELTGPWTGAKI 253
           G    + +PE  +L   +TE+++ +Y  +F K+GF G LN+YR ++  W+      G KI
Sbjct: 428 G-GLFVNSPEEPSLSRMVTEEEIQFYVQQFKKSGFRGPLNWYRNMERNWKWACKSLGRKI 487

Query: 254 KVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPHLEEVVVIEGAAHFINQEKADEINSL 313
            +P   +  ++D V   P   +++     ++ +PHL+    IE   H+   +K  E+N +
Sbjct: 488 LIPALMVTAEKDFVL-VPQMSQHM-----EDWIPHLKR-GHIEDCGHWTQMDKPTEVNQI 542

BLAST of CsGy4G000817 vs. ExPASy Swiss-Prot
Match: P80299 (Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.2e-44
Identity = 99/306 (32.35%), Postives = 161/306 (52.61%), Query Frame = 0

Query: 13  GINIHLASIGSGPALLFLHGFPEIWYTLRHQLLFFASRGFRAIAPDLRGYGDSDVPPSPS 72
           GI +H   +GSGPA+   HGFPE W++ R+Q+   A  GFR +A D++GYGDS  PP   
Sbjct: 245 GIRLHFVEMGSGPAICLCHGFPESWFSWRYQIPALAQAGFRVLAIDMKGYGDSSSPPEIE 304

Query: 73  SYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKALVNLSVYHTPRI 132
            Y    +  +++  L+ L I Q   +GHD    + W  ++F P+RV+A+ +L+    P  
Sbjct: 305 EYAMELLCEEMVTFLNKLGIPQAVFIGHDWAGVLVWNMALFHPERVRAVASLNTPLMPPN 364

Query: 133 PEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLRDPSAPIAPNGFS 192
           PE+ P+ +IR     ++ +  FQEPGVAEA+L   + +   K F    D    +  N  +
Sbjct: 365 PEVSPMEVIRSIPVFNYQL-YFQEPGVAEAEL-EKNMSRTFKSFFRTSDDMGLLTVNKAT 424

Query: 193 ----TLLATPE--TLPSWLTEDDVDYYASKFAKTGFTGGLNYYRALDLTWELTGPWTGAK 252
                L+ TPE   +    TE++++YY  +F K+GF G LN+YR  +  W+ +    G K
Sbjct: 425 EMGGILVGTPEDPKVSKITTEEEIEYYIQQFKKSGFRGPLNWYRNTERNWKWSCKALGRK 484

Query: 253 IKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPHLEEVVVIEGAAHFINQEKADEINS 312
           I VP   +  ++D+V     +K      + +  +P L+    IE   H+   EK  E+N 
Sbjct: 485 ILVPALMVTAEKDIVLRPEMSK------NMENWIPFLKR-GHIEDCGHWTQIEKPAEVNQ 541

BLAST of CsGy4G000817 vs. NCBI nr
Match: XP_004152244.1 (uncharacterized protein LOC101204815 [Cucumis sativus] >KGN52822.1 hypothetical protein Csa_014596 [Cucumis sativus])

HSP 1 Score: 652 bits (1681), Expect = 2.70e-236
Identity = 314/315 (99.68%), Postives = 314/315 (99.68%), Query Frame = 0

Query: 1   MEKIQHSTIPTNGINIHLASIGSGPALLFLHGFPEIWYTLRHQLLFFASRGFRAIAPDLR 60
           MEKIQHSTIPTNGINIHLASIGSGPALLFLHGFPEIWYT RHQLLFFASRGFRAIAPDLR
Sbjct: 1   MEKIQHSTIPTNGINIHLASIGSGPALLFLHGFPEIWYTWRHQLLFFASRGFRAIAPDLR 60

Query: 61  GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA 120
           GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA
Sbjct: 61  GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA 120

Query: 121 LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR 180
           LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR
Sbjct: 121 LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR 180

Query: 181 DPSAPIAPNGFSTLLATPETLPSWLTEDDVDYYASKFAKTGFTGGLNYYRALDLTWELTG 240
           DPSAPIAPNGFSTLLATPETLPSWLTEDDVDYYASKFAKTGFTGGLNYYRALDLTWELTG
Sbjct: 181 DPSAPIAPNGFSTLLATPETLPSWLTEDDVDYYASKFAKTGFTGGLNYYRALDLTWELTG 240

Query: 241 PWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPHLEEVVVIEGAAHFINQEK 300
           PWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPHLEEVVVIEGAAHFINQEK
Sbjct: 241 PWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPHLEEVVVIEGAAHFINQEK 300

Query: 301 ADEINSLIYDFITKF 315
           ADEINSLIYDFITKF
Sbjct: 301 ADEINSLIYDFITKF 315

BLAST of CsGy4G000817 vs. NCBI nr
Match: XP_008454327.1 (PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis melo] >KAA0044386.1 bifunctional epoxide hydrolase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 548 bits (1412), Expect = 2.70e-195
Identity = 258/315 (81.90%), Postives = 283/315 (89.84%), Query Frame = 0

Query: 1   MEKIQHSTIPTNGINIHLASIGSGPALLFLHGFPEIWYTLRHQLLFFASRGFRAIAPDLR 60
           M+KIQHSTI TNGINIH ASIGSGP +LFLHGFPE+WY+ RHQLLF AS+GFRAIAPDLR
Sbjct: 1   MDKIQHSTISTNGINIHFASIGSGPVVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60

Query: 61  GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA 120
           G+GDSD PPSPSSYT HHIVGDLIGLLDHL IDQVFLVGHD GA +AWYF +FRPDRVKA
Sbjct: 61  GFGDSDAPPSPSSYTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120

Query: 121 LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR 180
           LVNLSV++TPR P   PLA+ R +LGDDFYICKFQEPGVAEAD GSVDTATMMKKFLT+R
Sbjct: 121 LVNLSVHYTPRNPAGSPLAVTRRYLGDDFYICKFQEPGVAEADFGSVDTATMMKKFLTMR 180

Query: 181 DPSAPIAPNGFSTLLATPETLPSWLTEDDVDYYASKFAKTGFTGGLNYYRALDLTWELTG 240
           DP   I PNGF TLL TPE LPSWLTE+D++Y+ASKF+KTGFTGG NYYRALD+TWELTG
Sbjct: 181 DPRPAIIPNGFKTLLETPEILPSWLTEEDIEYFASKFSKTGFTGGFNYYRALDITWELTG 240

Query: 241 PWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPHLEEVVVIEGAAHFINQEK 300
           PW+ A+IKVPTKFIVGD DLVY+FPGAKEYIHG  FK+DVP LE+VVVIEGAAHFINQEK
Sbjct: 241 PWSRAQIKVPTKFIVGDLDLVYNFPGAKEYIHGGGFKKDVPLLEDVVVIEGAAHFINQEK 300

Query: 301 ADEINSLIYDFITKF 315
            DEI+SLIYDFITKF
Sbjct: 301 PDEISSLIYDFITKF 315

BLAST of CsGy4G000817 vs. NCBI nr
Match: XP_038905064.1 (epoxide hydrolase A-like [Benincasa hispida])

HSP 1 Score: 546 bits (1406), Expect = 2.14e-194
Identity = 260/315 (82.54%), Postives = 283/315 (89.84%), Query Frame = 0

Query: 1   MEKIQHSTIPTNGINIHLASIGSGPALLFLHGFPEIWYTLRHQLLFFASRGFRAIAPDLR 60
           M KIQHSTIPTNGINIH+ASIGSGPA+LFLHGFPE+WY+ RHQLLF AS+GFRAIAPDLR
Sbjct: 1   MNKIQHSTIPTNGINIHVASIGSGPAVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60

Query: 61  GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA 120
           G+GDSDV PSPSSYT HHIVGDLIGLLDHL IDQVFLVGHD GA +AWYF +FRPDRVKA
Sbjct: 61  GFGDSDVLPSPSSYTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120

Query: 121 LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR 180
           LVNLSV++TPR P + PLA +RL LGD+FY CKFQEPGVAEAD GSVDTATMMKKFLT+R
Sbjct: 121 LVNLSVHYTPRNPSISPLAGVRLLLGDNFYFCKFQEPGVAEADFGSVDTATMMKKFLTMR 180

Query: 181 DPSAPIAPNGFSTLLATPETLPSWLTEDDVDYYASKFAKTGFTGGLNYYRALDLTWELTG 240
           DPS PI PNGF TL ATPETLP WLTE+D+DY+ASKF KTGFTGG NYYRA DL+WELTG
Sbjct: 181 DPSPPIIPNGFKTL-ATPETLPPWLTEEDIDYFASKFGKTGFTGGFNYYRATDLSWELTG 240

Query: 241 PWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPHLEEVVVIEGAAHFINQEK 300
           PWTGA+IKVPTKFIVGD D+VYH PGAKEYIHG  FK+ VP LEEVVVIEGAAHFINQEK
Sbjct: 241 PWTGAQIKVPTKFIVGDLDMVYHLPGAKEYIHGGGFKKKVPLLEEVVVIEGAAHFINQEK 300

Query: 301 ADEINSLIYDFITKF 315
           A +I+SLIYDFI KF
Sbjct: 301 AYDISSLIYDFIKKF 314

BLAST of CsGy4G000817 vs. NCBI nr
Match: TYK29514.1 (bifunctional epoxide hydrolase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 535 bits (1379), Expect = 6.43e-190
Identity = 258/337 (76.56%), Postives = 283/337 (83.98%), Query Frame = 0

Query: 1   MEKIQHSTIPTNGINIHLASIGSGPALLFLHGFPEIWYTLRHQLLFFASRGFRAIAPDLR 60
           M+KIQHSTI TNGINIH ASIGSGP +LFLHGFPE+WY+ RHQLLF AS+GFRAIAPDLR
Sbjct: 1   MDKIQHSTISTNGINIHFASIGSGPVVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60

Query: 61  GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA 120
           G+GDSD PPSPSSYT HHIVGDLIGLLDHL IDQVFLVGHD GA +AWYF +FRPDRVKA
Sbjct: 61  GFGDSDAPPSPSSYTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120

Query: 121 LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQ----------------------EPG 180
           LVNLSV++TPR P   PLA+ R +LGDDFYICKFQ                      EPG
Sbjct: 121 LVNLSVHYTPRNPAGSPLAVTRRYLGDDFYICKFQVNAVDSLNSSVISEIEWLRSHWEPG 180

Query: 181 VAEADLGSVDTATMMKKFLTLRDPSAPIAPNGFSTLLATPETLPSWLTEDDVDYYASKFA 240
           VAEAD GSVDTATMMKKFLT+RDP   I PNGF TLL TPE LPSWLTE+D++Y+ASKF+
Sbjct: 181 VAEADFGSVDTATMMKKFLTMRDPRPAIIPNGFKTLLETPEILPSWLTEEDIEYFASKFS 240

Query: 241 KTGFTGGLNYYRALDLTWELTGPWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKE 300
           KTGFTGG NYYRALD+TWELTGPW+ A+IKVPTKFIVGD DLVY+FPGAKEYIHG  FK+
Sbjct: 241 KTGFTGGFNYYRALDITWELTGPWSRAQIKVPTKFIVGDLDLVYNFPGAKEYIHGGGFKK 300

Query: 301 DVPHLEEVVVIEGAAHFINQEKADEINSLIYDFITKF 315
           DVP LE+VVVIEGAAHFINQEK DEI+SLIYDFITKF
Sbjct: 301 DVPLLEDVVVIEGAAHFINQEKPDEISSLIYDFITKF 337

BLAST of CsGy4G000817 vs. NCBI nr
Match: XP_022934351.1 (uncharacterized protein LOC111441542 [Cucurbita moschata])

HSP 1 Score: 534 bits (1375), Expect = 1.13e-189
Identity = 250/315 (79.37%), Postives = 281/315 (89.21%), Query Frame = 0

Query: 1   MEKIQHSTIPTNGINIHLASIGSGPALLFLHGFPEIWYTLRHQLLFFASRGFRAIAPDLR 60
           MEKI+HSTI TNGIN+H+ASIGSGP +LFLHGFPE+WY+ RHQLLF AS+GFRAIAPDLR
Sbjct: 1   MEKIEHSTIATNGINMHVASIGSGPPVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60

Query: 61  GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA 120
           G+GDSDVPPSPSSYT  HI+GDLIGLLDHL I+QVFLVGHD GA +AWYF +FRPDRVKA
Sbjct: 61  GFGDSDVPPSPSSYTPFHIIGDLIGLLDHLGIEQVFLVGHDWGAMMAWYFCLFRPDRVKA 120

Query: 121 LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR 180
           LVNLSV++ PR P + PL+  R FLGDDFYICKFQ PGVAEAD GSVDTATMMKKFLT+R
Sbjct: 121 LVNLSVHYNPRNPAISPLSRTRQFLGDDFYICKFQTPGVAEADFGSVDTATMMKKFLTIR 180

Query: 181 DPSAPIAPNGFSTLLATPETLPSWLTEDDVDYYASKFAKTGFTGGLNYYRALDLTWELTG 240
           DPS PI PNGF TL  TPETLPSWLTE+D+DY+ASKF KTGFTGG NYYRA++ TWELTG
Sbjct: 181 DPSPPIIPNGFKTL-KTPETLPSWLTEEDIDYFASKFTKTGFTGGFNYYRAIEQTWELTG 240

Query: 241 PWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPHLEEVVVIEGAAHFINQEK 300
           PW+GAKIKVPTK++VGD D+VYH PGAK+YIHG  FK+DVP LEEVVV+EGAAHFINQEK
Sbjct: 241 PWSGAKIKVPTKYVVGDVDMVYHLPGAKQYIHGGGFKKDVPLLEEVVVMEGAAHFINQEK 300

Query: 301 ADEINSLIYDFITKF 315
           ADEI++ IYDFI KF
Sbjct: 301 ADEISAHIYDFIIKF 314

BLAST of CsGy4G000817 vs. ExPASy TrEMBL
Match: A0A0A0KWK9 (Epoxide hydrolase 2 OS=Cucumis sativus OX=3659 GN=Csa_4G001890 PE=4 SV=1)

HSP 1 Score: 652 bits (1681), Expect = 1.31e-236
Identity = 314/315 (99.68%), Postives = 314/315 (99.68%), Query Frame = 0

Query: 1   MEKIQHSTIPTNGINIHLASIGSGPALLFLHGFPEIWYTLRHQLLFFASRGFRAIAPDLR 60
           MEKIQHSTIPTNGINIHLASIGSGPALLFLHGFPEIWYT RHQLLFFASRGFRAIAPDLR
Sbjct: 1   MEKIQHSTIPTNGINIHLASIGSGPALLFLHGFPEIWYTWRHQLLFFASRGFRAIAPDLR 60

Query: 61  GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA 120
           GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA
Sbjct: 61  GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA 120

Query: 121 LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR 180
           LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR
Sbjct: 121 LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR 180

Query: 181 DPSAPIAPNGFSTLLATPETLPSWLTEDDVDYYASKFAKTGFTGGLNYYRALDLTWELTG 240
           DPSAPIAPNGFSTLLATPETLPSWLTEDDVDYYASKFAKTGFTGGLNYYRALDLTWELTG
Sbjct: 181 DPSAPIAPNGFSTLLATPETLPSWLTEDDVDYYASKFAKTGFTGGLNYYRALDLTWELTG 240

Query: 241 PWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPHLEEVVVIEGAAHFINQEK 300
           PWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPHLEEVVVIEGAAHFINQEK
Sbjct: 241 PWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPHLEEVVVIEGAAHFINQEK 300

Query: 301 ADEINSLIYDFITKF 315
           ADEINSLIYDFITKF
Sbjct: 301 ADEINSLIYDFITKF 315

BLAST of CsGy4G000817 vs. ExPASy TrEMBL
Match: A0A5A7TQJ9 (Bifunctional epoxide hydrolase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G001150 PE=4 SV=1)

HSP 1 Score: 548 bits (1412), Expect = 1.31e-195
Identity = 258/315 (81.90%), Postives = 283/315 (89.84%), Query Frame = 0

Query: 1   MEKIQHSTIPTNGINIHLASIGSGPALLFLHGFPEIWYTLRHQLLFFASRGFRAIAPDLR 60
           M+KIQHSTI TNGINIH ASIGSGP +LFLHGFPE+WY+ RHQLLF AS+GFRAIAPDLR
Sbjct: 1   MDKIQHSTISTNGINIHFASIGSGPVVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60

Query: 61  GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA 120
           G+GDSD PPSPSSYT HHIVGDLIGLLDHL IDQVFLVGHD GA +AWYF +FRPDRVKA
Sbjct: 61  GFGDSDAPPSPSSYTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120

Query: 121 LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR 180
           LVNLSV++TPR P   PLA+ R +LGDDFYICKFQEPGVAEAD GSVDTATMMKKFLT+R
Sbjct: 121 LVNLSVHYTPRNPAGSPLAVTRRYLGDDFYICKFQEPGVAEADFGSVDTATMMKKFLTMR 180

Query: 181 DPSAPIAPNGFSTLLATPETLPSWLTEDDVDYYASKFAKTGFTGGLNYYRALDLTWELTG 240
           DP   I PNGF TLL TPE LPSWLTE+D++Y+ASKF+KTGFTGG NYYRALD+TWELTG
Sbjct: 181 DPRPAIIPNGFKTLLETPEILPSWLTEEDIEYFASKFSKTGFTGGFNYYRALDITWELTG 240

Query: 241 PWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPHLEEVVVIEGAAHFINQEK 300
           PW+ A+IKVPTKFIVGD DLVY+FPGAKEYIHG  FK+DVP LE+VVVIEGAAHFINQEK
Sbjct: 241 PWSRAQIKVPTKFIVGDLDLVYNFPGAKEYIHGGGFKKDVPLLEDVVVIEGAAHFINQEK 300

Query: 301 ADEINSLIYDFITKF 315
            DEI+SLIYDFITKF
Sbjct: 301 PDEISSLIYDFITKF 315

BLAST of CsGy4G000817 vs. ExPASy TrEMBL
Match: A0A1S3BZ44 (bifunctional epoxide hydrolase 2-like OS=Cucumis melo OX=3656 GN=LOC103494761 PE=4 SV=1)

HSP 1 Score: 548 bits (1412), Expect = 1.31e-195
Identity = 258/315 (81.90%), Postives = 283/315 (89.84%), Query Frame = 0

Query: 1   MEKIQHSTIPTNGINIHLASIGSGPALLFLHGFPEIWYTLRHQLLFFASRGFRAIAPDLR 60
           M+KIQHSTI TNGINIH ASIGSGP +LFLHGFPE+WY+ RHQLLF AS+GFRAIAPDLR
Sbjct: 1   MDKIQHSTISTNGINIHFASIGSGPVVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60

Query: 61  GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA 120
           G+GDSD PPSPSSYT HHIVGDLIGLLDHL IDQVFLVGHD GA +AWYF +FRPDRVKA
Sbjct: 61  GFGDSDAPPSPSSYTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120

Query: 121 LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR 180
           LVNLSV++TPR P   PLA+ R +LGDDFYICKFQEPGVAEAD GSVDTATMMKKFLT+R
Sbjct: 121 LVNLSVHYTPRNPAGSPLAVTRRYLGDDFYICKFQEPGVAEADFGSVDTATMMKKFLTMR 180

Query: 181 DPSAPIAPNGFSTLLATPETLPSWLTEDDVDYYASKFAKTGFTGGLNYYRALDLTWELTG 240
           DP   I PNGF TLL TPE LPSWLTE+D++Y+ASKF+KTGFTGG NYYRALD+TWELTG
Sbjct: 181 DPRPAIIPNGFKTLLETPEILPSWLTEEDIEYFASKFSKTGFTGGFNYYRALDITWELTG 240

Query: 241 PWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPHLEEVVVIEGAAHFINQEK 300
           PW+ A+IKVPTKFIVGD DLVY+FPGAKEYIHG  FK+DVP LE+VVVIEGAAHFINQEK
Sbjct: 241 PWSRAQIKVPTKFIVGDLDLVYNFPGAKEYIHGGGFKKDVPLLEDVVVIEGAAHFINQEK 300

Query: 301 ADEINSLIYDFITKF 315
            DEI+SLIYDFITKF
Sbjct: 301 PDEISSLIYDFITKF 315

BLAST of CsGy4G000817 vs. ExPASy TrEMBL
Match: A0A5D3E108 (Bifunctional epoxide hydrolase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001170 PE=4 SV=1)

HSP 1 Score: 535 bits (1379), Expect = 3.11e-190
Identity = 258/337 (76.56%), Postives = 283/337 (83.98%), Query Frame = 0

Query: 1   MEKIQHSTIPTNGINIHLASIGSGPALLFLHGFPEIWYTLRHQLLFFASRGFRAIAPDLR 60
           M+KIQHSTI TNGINIH ASIGSGP +LFLHGFPE+WY+ RHQLLF AS+GFRAIAPDLR
Sbjct: 1   MDKIQHSTISTNGINIHFASIGSGPVVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60

Query: 61  GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA 120
           G+GDSD PPSPSSYT HHIVGDLIGLLDHL IDQVFLVGHD GA +AWYF +FRPDRVKA
Sbjct: 61  GFGDSDAPPSPSSYTPHHIVGDLIGLLDHLGIDQVFLVGHDWGAMMAWYFCLFRPDRVKA 120

Query: 121 LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQ----------------------EPG 180
           LVNLSV++TPR P   PLA+ R +LGDDFYICKFQ                      EPG
Sbjct: 121 LVNLSVHYTPRNPAGSPLAVTRRYLGDDFYICKFQVNAVDSLNSSVISEIEWLRSHWEPG 180

Query: 181 VAEADLGSVDTATMMKKFLTLRDPSAPIAPNGFSTLLATPETLPSWLTEDDVDYYASKFA 240
           VAEAD GSVDTATMMKKFLT+RDP   I PNGF TLL TPE LPSWLTE+D++Y+ASKF+
Sbjct: 181 VAEADFGSVDTATMMKKFLTMRDPRPAIIPNGFKTLLETPEILPSWLTEEDIEYFASKFS 240

Query: 241 KTGFTGGLNYYRALDLTWELTGPWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKE 300
           KTGFTGG NYYRALD+TWELTGPW+ A+IKVPTKFIVGD DLVY+FPGAKEYIHG  FK+
Sbjct: 241 KTGFTGGFNYYRALDITWELTGPWSRAQIKVPTKFIVGDLDLVYNFPGAKEYIHGGGFKK 300

Query: 301 DVPHLEEVVVIEGAAHFINQEKADEINSLIYDFITKF 315
           DVP LE+VVVIEGAAHFINQEK DEI+SLIYDFITKF
Sbjct: 301 DVPLLEDVVVIEGAAHFINQEKPDEISSLIYDFITKF 337

BLAST of CsGy4G000817 vs. ExPASy TrEMBL
Match: A0A6J1F1K3 (uncharacterized protein LOC111441542 OS=Cucurbita moschata OX=3662 GN=LOC111441542 PE=4 SV=1)

HSP 1 Score: 534 bits (1375), Expect = 5.46e-190
Identity = 250/315 (79.37%), Postives = 281/315 (89.21%), Query Frame = 0

Query: 1   MEKIQHSTIPTNGINIHLASIGSGPALLFLHGFPEIWYTLRHQLLFFASRGFRAIAPDLR 60
           MEKI+HSTI TNGIN+H+ASIGSGP +LFLHGFPE+WY+ RHQLLF AS+GFRAIAPDLR
Sbjct: 1   MEKIEHSTIATNGINMHVASIGSGPPVLFLHGFPELWYSWRHQLLFLASKGFRAIAPDLR 60

Query: 61  GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA 120
           G+GDSDVPPSPSSYT  HI+GDLIGLLDHL I+QVFLVGHD GA +AWYF +FRPDRVKA
Sbjct: 61  GFGDSDVPPSPSSYTPFHIIGDLIGLLDHLGIEQVFLVGHDWGAMMAWYFCLFRPDRVKA 120

Query: 121 LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR 180
           LVNLSV++ PR P + PL+  R FLGDDFYICKFQ PGVAEAD GSVDTATMMKKFLT+R
Sbjct: 121 LVNLSVHYNPRNPAISPLSRTRQFLGDDFYICKFQTPGVAEADFGSVDTATMMKKFLTIR 180

Query: 181 DPSAPIAPNGFSTLLATPETLPSWLTEDDVDYYASKFAKTGFTGGLNYYRALDLTWELTG 240
           DPS PI PNGF TL  TPETLPSWLTE+D+DY+ASKF KTGFTGG NYYRA++ TWELTG
Sbjct: 181 DPSPPIIPNGFKTL-KTPETLPSWLTEEDIDYFASKFTKTGFTGGFNYYRAIEQTWELTG 240

Query: 241 PWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPHLEEVVVIEGAAHFINQEK 300
           PW+GAKIKVPTK++VGD D+VYH PGAK+YIHG  FK+DVP LEEVVV+EGAAHFINQEK
Sbjct: 241 PWSGAKIKVPTKYVVGDVDMVYHLPGAKQYIHGGGFKKDVPLLEEVVVMEGAAHFINQEK 300

Query: 301 ADEINSLIYDFITKF 315
           ADEI++ IYDFI KF
Sbjct: 301 ADEISAHIYDFIIKF 314

BLAST of CsGy4G000817 vs. TAIR 10
Match: AT4G02340.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 418.3 bits (1074), Expect = 5.4e-117
Identity = 193/317 (60.88%), Postives = 235/317 (74.13%), Query Frame = 0

Query: 1   MEKIQHSTIPTNGINIHLASIGSGPALLFLHGFPEIWYTLRHQLLFFASRGFRAIAPDLR 60
           MEKI+H+TI TNGIN+H+ASIGSGP +LF+HGFP++WY+ RHQL+ FA+ G+RAIAPDLR
Sbjct: 1   MEKIEHTTISTNGINMHVASIGSGPVILFVHGFPDLWYSWRHQLVSFAALGYRAIAPDLR 60

Query: 61  GYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQVFLVGHDLGATIAWYFSIFRPDRVKA 120
           GYGDSD PPS  SYT  HIVGDL+GLLD L +D+VFLVGHD GA +AW+  + RPDRV A
Sbjct: 61  GYGDSDAPPSRESYTILHIVGDLVGLLDSLGVDRVFLVGHDWGAIVAWWLCMIRPDRVNA 120

Query: 121 LVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFLTLR 180
           LVN SV   PR P + P+   R   GDD+YIC+FQEPG  E D   VDT  ++ +F T R
Sbjct: 121 LVNTSVVFNPRNPSVKPVDAFRALFGDDYYICRFQEPGEIEEDFAQVDTKKLITRFFTSR 180

Query: 181 DPSAPIAPN--GFSTLLATPETLPSWLTEDDVDYYASKFAKTGFTGGLNYYRALDLTWEL 240
           +P  P  P   GF   L  P +LP+WLTE DV +Y  KF++ GFTGGLNYYRAL+L+WEL
Sbjct: 181 NPRPPCIPKSVGFRG-LPDPPSLPAWLTEQDVRFYGDKFSQKGFTGGLNYYRALNLSWEL 240

Query: 241 TGPWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPHLEEVVVIEGAAHFINQ 300
           T PWTG +IKVP KFIVGD D+ Y+ PG KEYIH    K+ VP L+EVVV+EG  HF++Q
Sbjct: 241 TAPWTGLQIKVPVKFIVGDLDITYNIPGTKEYIHEGGLKKHVPFLQEVVVMEGVGHFLHQ 300

Query: 301 EKADEINSLIYDFITKF 316
           EK DE+   IY F  KF
Sbjct: 301 EKPDEVTDHIYGFFKKF 316

BLAST of CsGy4G000817 vs. TAIR 10
Match: AT2G26740.1 (soluble epoxide hydrolase )

HSP 1 Score: 363.6 bits (932), Expect = 1.6e-100
Identity = 182/321 (56.70%), Postives = 227/321 (70.72%), Query Frame = 0

Query: 4   IQHSTIPTNGINIHLASIG--SGPALLFLHGFPEIWYTLRHQLLFFASRGFRAIAPDLRG 63
           ++H  +  NGI+IH+A  G   GP +L LHGFPE+WY+ RHQ+   A+RG+RA+APDLRG
Sbjct: 1   MEHRKVRGNGIDIHVAIQGPSDGPIVLLLHGFPELWYSWRHQIPGLAARGYRAVAPDLRG 60

Query: 64  YGDSDVPPSPSSYTAHHIVGDLIGLLDHL---EIDQVFLVGHDLGATIAWYFSIFRPDRV 123
           YGDSD P   SSYT  +IVGDLI ++  L   E ++VF+VGHD GA IAWY  +FRPDRV
Sbjct: 61  YGDSDAPAEISSYTCFNIVGDLIAVISALTASEDEKVFVVGHDWGALIAWYLCLFRPDRV 120

Query: 124 KALVNLSVYHT--PRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKF 183
           KALVNLSV  +  P  P + P+  +R F GDD+YIC+FQE G  EA++  V T  +MK+ 
Sbjct: 121 KALVNLSVPFSFRPTDPSVKPVDRMRAFYGDDYYICRFQEFGDVEAEIAEVGTERVMKRL 180

Query: 184 LTLRDPSAPIAPNGFSTLLATPET--LPSWLTEDDVDYYASKFAKTGFTGGLNYYRALDL 243
           LT R P   I P   S   +  ET  LPSWLTE+DV Y+ SKF + GF+G +NYYR  + 
Sbjct: 181 LTYRTPGPVIIPKDKSFWGSKGETIPLPSWLTEEDVAYFVSKFEEKGFSGPVNYYRNFNR 240

Query: 244 TWELTGPWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPHLEEVVVIEGAAH 303
             EL GPW G+KI+VPTKF++G+ DLVY+ PG KEYIHG  FKEDVP LEE VV+EG AH
Sbjct: 241 NNELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLLEEPVVMEGVAH 300

Query: 304 FINQEKADEINSLIYDFITKF 316
           FINQEK  EI  +I DFI+KF
Sbjct: 301 FINQEKPQEILQIILDFISKF 321

BLAST of CsGy4G000817 vs. TAIR 10
Match: AT3G05600.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 349.4 bits (895), Expect = 3.1e-96
Identity = 169/324 (52.16%), Postives = 214/324 (66.05%), Query Frame = 0

Query: 1   MEKIQHSTIPTNGINIHLASIG--SGPALLFLHGFPEIWYTLRHQLLFFASRGFRAIAPD 60
           ME I H  +  NGI +H+A  G   GP +L LHGFP++WYT RHQ+   +S G+RA+APD
Sbjct: 1   MEGIDHRMVSVNGITMHIAEKGPKEGPVVLLLHGFPDLWYTWRHQISGLSSLGYRAVAPD 60

Query: 61  LRGYGDSDVPPSPSSYTAHHIVGDLIGLLDHLEIDQ--VFLVGHDLGATIAWYFSIFRPD 120
           LRGYGDSD P S S YT  ++VGDL+ LLD +  +Q  VFLVGHD GA I W+  +FRP+
Sbjct: 61  LRGYGDSDSPESFSEYTCLNVVGDLVALLDSVAGNQEKVFLVGHDWGAIIGWFLCLFRPE 120

Query: 121 RVKALVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKF 180
           ++   V LSV +  R P++ P+   +   GDD+YIC+FQEPG  E ++ S D    ++  
Sbjct: 121 KINGFVCLSVPYRSRNPKVKPVQGFKAVFGDDYYICRFQEPGKIEGEIASADPRIFLRNL 180

Query: 181 LTLRDPSAPIAP--NGFSTLLATPET----LPSWLTEDDVDYYASKFAKTGFTGGLNYYR 240
            T R    PI P  N F      P +    LP W ++ D+D+Y SKF K GFTGGLNYYR
Sbjct: 181 FTGRTLGPPILPKDNPFGE-KPNPNSENIELPEWFSKKDLDFYVSKFEKAGFTGGLNYYR 240

Query: 241 ALDLTWELTGPWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPHLEEVVVIE 300
           A+DL WELT PWTGAKI+VP KF+ GD D+VY  PG KEYIHG  F  DVP L+E+VVIE
Sbjct: 241 AMDLNWELTAPWTGAKIQVPVKFMTGDFDMVYTTPGMKEYIHGGGFAADVPTLQEIVVIE 300

Query: 301 GAAHFINQEKADEINSLIYDFITK 315
            A HF+NQEK  E+ + I DF TK
Sbjct: 301 DAGHFVNQEKPQEVTAHINDFFTK 323

BLAST of CsGy4G000817 vs. TAIR 10
Match: AT2G26750.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 347.1 bits (889), Expect = 1.5e-95
Identity = 172/320 (53.75%), Postives = 221/320 (69.06%), Query Frame = 0

Query: 4   IQHSTIPTNGINIHLASIG--SGPALLFLHGFPEIWYTLRHQLLFFASRGFRAIAPDLRG 63
           ++H  +  NGI+IH+A  G   G  +L LHGFPE+WY+ RHQ+   A+RG+RA+APDLRG
Sbjct: 1   MEHRNVRGNGIDIHVAIQGPSDGTIVLLLHGFPELWYSWRHQISGLAARGYRAVAPDLRG 60

Query: 64  YGDSDVPPSPSSYTAHHIVGDLIGLLDHL--EIDQVFLVGHDLGATIAWYFSIFRPDRVK 123
           YGDSD P   SS+T  +IVGDL+ ++  L  E  +VF+VGHD GA IAWY  +FRPD+VK
Sbjct: 61  YGDSDAPAEISSFTCFNIVGDLVAVISTLIKEDKKVFVVGHDWGALIAWYLCLFRPDKVK 120

Query: 124 ALVNLSV--YHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMMKKFL 183
           ALVNLSV     P  P + P+  +R   G+D+Y+C+FQE G  EA++  V T  +MK+ L
Sbjct: 121 ALVNLSVPLSFWPTDPSVKPVDRMRAVYGNDYYVCRFQEVGDIEAEIAEVGTERVMKRLL 180

Query: 184 TLRDPSAPIAPNGFSTLLATPET--LPSWLTEDDVDYYASKFAKTGFTGGLNYYRALDLT 243
           T R P   I P   S   +  ET  LPSWLTE+DV Y+ SKF + GF G +NYYR  +  
Sbjct: 181 TYRTPGPLIIPKDKSFWGSKGETIPLPSWLTEEDVAYFVSKFKEKGFCGPVNYYRNFNRN 240

Query: 244 WELTGPWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPHLEEVVVIEGAAHF 303
            EL GPW G+KI+VPTKF++G+ DLVY+ PG KEYIHG  FKEDVP +EE VV+EG AHF
Sbjct: 241 NELLGPWVGSKIQVPTKFVIGELDLVYYMPGVKEYIHGPQFKEDVPLIEEPVVMEGVAHF 300

Query: 304 INQEKADEINSLIYDFITKF 316
           +NQEK  EI  +I DFI+ F
Sbjct: 301 LNQEKPQEILQIILDFISTF 320

BLAST of CsGy4G000817 vs. TAIR 10
Match: AT4G15960.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 338.2 bits (866), Expect = 7.2e-93
Identity = 156/324 (48.15%), Postives = 214/324 (66.05%), Query Frame = 0

Query: 1   MEKIQHSTIPTNGINIHLA-----SIGSGPALLFLHGFPEIWYTLRHQLLFFASRGFRAI 60
           ++ ++H T+  NGIN+H+A       G  P +LFLHGFPE+WYT RHQ++  +S G+R I
Sbjct: 51  LDGVEHKTLKVNGINMHVAEKPGSGSGEDPIILFLHGFPELWYTWRHQMVALSSLGYRTI 110

Query: 61  APDLRGYGDSDVPPSPSSYTAHHIVGDLIGLLDHLE--IDQVFLVGHDLGATIAWYFSIF 120
           APDLRGYGD++ P     YT  ++ GD++ L+D +      V +VGHD GA IAW    +
Sbjct: 111 APDLRGYGDTEAPEKVEDYTYLNVDGDVVALIDAVTGGDKAVSVVGHDWGAMIAWQLCQY 170

Query: 121 RPDRVKALVNLSVYHTPRIPEMPPLAIIRLFLGDDFYICKFQEPGVAEADLGSVDTATMM 180
           RP++VKALVN+SV  +PR P   P+  +R   GDD+Y+C+FQ+ G  E +   + T  ++
Sbjct: 171 RPEKVKALVNMSVLFSPRNPVRVPVPTLRHVFGDDYYVCRFQKAGEIETEFKKLGTENVL 230

Query: 181 KKFLTLRDPSAPIAPNG--FSTLLATPETLPSWLTEDDVDYYASKFAKTGFTGGLNYYRA 240
           K+FLT + P     P    F         LP WLT++D+DYY +K+   GFTG +NYYR 
Sbjct: 231 KEFLTYKTPGPLNLPKDKYFKRSENAASALPLWLTQEDLDYYVTKYENKGFTGPINYYRN 290

Query: 241 LDLTWELTGPWTGAKIKVPTKFIVGDQDLVYHFPGAKEYIHGDSFKEDVPHLEEVVVIEG 300
           +D  WELT PWTGAKI+VP KFI+GDQDL Y+FPGAKEYI+G  FK DVP L+E VV++G
Sbjct: 291 IDRNWELTAPWTGAKIRVPVKFIIGDQDLTYNFPGAKEYINGGGFKRDVPLLDETVVLKG 350

Query: 301 AAHFINQEKADEINSLIYDFITKF 316
             HF+++E  D IN  I++F  KF
Sbjct: 351 LGHFLHEENPDVINQHIHNFFHKF 374

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
I6YGS07.3e-5837.93Epoxide hydrolase A OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX... [more]
Q6Q2C22.9e-4632.68Bifunctional epoxide hydrolase 2 OS=Sus scrofa OX=9823 GN=EPHX2 PE=2 SV=1[more]
P349148.4e-4633.33Bifunctional epoxide hydrolase 2 OS=Mus musculus OX=10090 GN=Ephx2 PE=1 SV=2[more]
P349131.2e-4431.48Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2[more]
P802991.2e-4432.35Bifunctional epoxide hydrolase 2 OS=Rattus norvegicus OX=10116 GN=Ephx2 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
XP_004152244.12.70e-23699.68uncharacterized protein LOC101204815 [Cucumis sativus] >KGN52822.1 hypothetical ... [more]
XP_008454327.12.70e-19581.90PREDICTED: bifunctional epoxide hydrolase 2-like [Cucumis melo] >KAA0044386.1 bi... [more]
XP_038905064.12.14e-19482.54epoxide hydrolase A-like [Benincasa hispida][more]
TYK29514.16.43e-19076.56bifunctional epoxide hydrolase 2-like [Cucumis melo var. makuwa][more]
XP_022934351.11.13e-18979.37uncharacterized protein LOC111441542 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A0A0KWK91.31e-23699.68Epoxide hydrolase 2 OS=Cucumis sativus OX=3659 GN=Csa_4G001890 PE=4 SV=1[more]
A0A5A7TQJ91.31e-19581.90Bifunctional epoxide hydrolase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3BZ441.31e-19581.90bifunctional epoxide hydrolase 2-like OS=Cucumis melo OX=3656 GN=LOC103494761 PE... [more]
A0A5D3E1083.11e-19076.56Bifunctional epoxide hydrolase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A6J1F1K35.46e-19079.37uncharacterized protein LOC111441542 OS=Cucurbita moschata OX=3662 GN=LOC1114415... [more]
Match NameE-valueIdentityDescription
AT4G02340.15.4e-11760.88alpha/beta-Hydrolases superfamily protein [more]
AT2G26740.11.6e-10056.70soluble epoxide hydrolase [more]
AT3G05600.13.1e-9652.16alpha/beta-Hydrolases superfamily protein [more]
AT2G26750.11.5e-9553.75alpha/beta-Hydrolases superfamily protein [more]
AT4G15960.17.2e-9348.15alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000639Epoxide hydrolase-likePRINTSPR00412EPOXHYDRLASEcoord: 289..311
score: 39.33
coord: 51..66
score: 62.5
coord: 31..49
score: 43.54
coord: 111..124
score: 49.68
coord: 97..110
score: 44.81
coord: 249..265
score: 43.05
IPR000073Alpha/beta hydrolase fold-1PRINTSPR00111ABHYDROLASEcoord: 97..110
score: 32.51
coord: 111..124
score: 32.02
coord: 51..66
score: 46.55
IPR000073Alpha/beta hydrolase fold-1PFAMPF00561Abhydrolase_1coord: 25..297
e-value: 2.1E-21
score: 76.7
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 1..315
e-value: 7.8E-106
score: 356.4
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 2..314
NoneNo IPR availablePANTHERPTHR43329:SF58OS05G0273800 PROTEINcoord: 1..315
NoneNo IPR availablePANTHERPTHR43329EPOXIDE HYDROLASEcoord: 1..315

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy4G000817.1CsGy4G000817.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0003824 catalytic activity