Homology
BLAST of CsGy3G044370 vs. ExPASy Swiss-Prot
Match:
B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)
HSP 1 Score: 416.4 bits (1069), Expect = 4.5e-115
Identity = 223/499 (44.69%), Postives = 327/499 (65.53%), Query Frame = 0
Query: 1 MEMISVCVIIGVVLGVCLL-----VRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGYSFS 60
ME+ VC+ +L VC + VR LN +Y VKL R LPPGD+GWP+IG S
Sbjct: 1 MEVHWVCMSAATLL-VCYIFGSKFVRNLNGWYYDVKL-RRKEHPLPPGDMGWPLIGDLLS 60
Query: 61 SYKTFIVQEDPISFIQSLHSRYGKGGMYKTHLYGNPTVIVTNPEICRRIYLDEANFKQHY 120
K F P SFI +L +YG+ G+YKTHL+GNP++IV P++CRR+ D+ NFK Y
Sbjct: 61 FIKDF-SSGHPDSFINNLVLKYGRSGIYKTHLFGNPSIIVCEPQMCRRVLTDDVNFKLGY 120
Query: 121 PKSVKILEGSSG--DFSNMDHKIAYKVMASPMNGYEVLSNYVDFIEEVIAKGLEEWSSMM 180
PKS+K L D SN +H++ +++ SP+ G++ L+ Y++ +EE++ LEE SS M
Sbjct: 121 PKSIKELARCRPMIDVSNAEHRLFRRLITSPIVGHKALAMYLERLEEIVINSLEELSS-M 180
Query: 181 REPIKLVDEIGILFFKVITKIFLGSQLDAKTMVELHTLYKELSFGMVMSTFPYDFPGFTF 240
+ P++L+ E+ + FK I +F+GS + + ++ + + +L GM + P + PGFTF
Sbjct: 181 KHPVELLKEMKKVSFKAIVHVFMGSS-NQDIIKKIGSSFTDLYNGMF--SIPINVPGFTF 240
Query: 241 HQLLKARRKIENTIQGVVEEKIRRFEN--DKTSEVQCQVDKLVVAINENGAKLYSNNFIR 300
H+ L+AR+K+ +Q VV+E+ EN + S+ + +D L+ +ENG KL + I
Sbjct: 241 HKALEARKKLAKIVQPVVDERRLMIENGPQEGSQRKDLIDILLEVKDENGRKLEDED-IS 300
Query: 301 DLILGIFFAGHSTPAIAACWALLHISQNPHVFQKAKEEQESIIRQRPSVQKGLTLNEIKQ 360
DL++G+ FAGH + A + W++ +++Q+PH+ +KAKEEQE I R R S QK L+L EIKQ
Sbjct: 301 DLLIGLLFAGHESTATSLMWSITYLTQHPHILKKAKEEQEEITRTRFSSQKQLSLKEIKQ 360
Query: 361 MKYLTKIINEVLRRNTITATNFRETKTDVNINGHFIPKGWTVQIWNIAVHMDPQIYSNPQ 420
M YL+++I+E LR I FRE DVNING+ IPKGW V IW A+HMD + Y NP+
Sbjct: 361 MVYLSQVIDETLRCANIAFATFREATADVNINGYIIPKGWRVLIWARAIHMDSEYYPNPE 420
Query: 421 EFNPSRWDNYTPKPGEFIPFGLGSRFCPGSELAKLEITILLHHFILNYKMEGVNLESNNN 480
EFNPSRWD+Y K G F+PFG GSR CPG++LAKLEI+I LH+F+ NY++E +N E +
Sbjct: 421 EFNPSRWDDYNAKAGTFLPFGAGSRLCPGADLAKLEISIFLHYFLRNYRLERINPECHVT 480
Query: 481 QMPSPRP-ENCLCKIIRLS 490
+P +P +NCL K+I++S
Sbjct: 481 SLPVSKPTDNCLAKVIKVS 491
BLAST of CsGy3G044370 vs. ExPASy Swiss-Prot
Match:
Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)
HSP 1 Score: 365.5 bits (937), Expect = 9.1e-100
Identity = 206/482 (42.74%), Postives = 297/482 (61.62%), Query Frame = 0
Query: 12 VVLGVCLL---VRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGYSFSSYKTFIVQEDPIS 71
++LG+ +L ++R+N Y+ KLG + + LPPGDLGWPVIG +S + F DP S
Sbjct: 14 IILGLFVLKWVLKRVNVWIYVSKLGEKKH-YLPPGDLGWPVIGNMWSFLRAF-KTSDPES 73
Query: 72 FIQSLHSRYGKGGMYKTHLYGNPTVIVTNPEICRRIYLDEANFKQHYPKSVKILEG--SS 131
FIQS +RYG+ G+YK H++G P V+VT PE CRR+ D+ F +PKS L G S
Sbjct: 74 FIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSF 133
Query: 132 GDFSNMDHKIAYKVMASPMNGYEVLSNYVDFIEEVIAKGLEEWSSMMREPIKLVDEIGIL 191
S +HK ++ ++P+NG E LS Y+ FIEE + LE+WS M I+ + + L
Sbjct: 134 VGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKM--GEIEFLSHLRKL 193
Query: 192 FFKVITKIFLGSQLDAKTMVELHTLYKELSFGMVMSTFPYDFPGFTFHQLLKARRKIENT 251
FKVI IFL S+ + M L Y L++G + + PGF +H+ LKAR+K+
Sbjct: 194 TFKVIMYIFLSSESE-HVMDSLEREYTNLNYG--VRAMGINLPGFAYHRALKARKKLVAA 253
Query: 252 IQGVVEEKIRRFENDKTSEVQCQVDKLVVAINENGAKLYSNNFIRDLILGIFFAGHSTPA 311
Q +V + + + + +S + +D L+ +ENG L I DL+L AGH +
Sbjct: 254 FQSIVTNRRNQRKQNISSNRKDMLDNLIDVKDENGRVLDDEEII-DLLLMYLNAGHESSG 313
Query: 312 IAACWALLHISQNPHVFQKAKEEQESIIRQRPSVQKGLTLNEIKQMKYLTKIINEVLRRN 371
WA + + ++P + QKAKEEQE I+++R QK LTL E ++M YL+++I+E LR
Sbjct: 314 HLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVI 373
Query: 372 TITATNFRETKTDVNINGHFIPKGWTVQIWNIAVHMDPQIYSNPQEFNPSRWDNYTPKPG 431
T + T FRE K+DV ++G+ IPKGW V W VH+DP+IY +P++F+PSRW+ YTPK G
Sbjct: 374 TFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAG 433
Query: 432 EFIPFGLGSRFCPGSELAKLEITILLHHFILNYKMEGVNLESNNNQMPSPRP-ENCLCKI 488
F+PFGLGS CPG++LAKLEI+I LHHF+L Y++E N +P RP +NCL +I
Sbjct: 434 TFLPFGLGSHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARI 486
BLAST of CsGy3G044370 vs. ExPASy Swiss-Prot
Match:
Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)
HSP 1 Score: 364.4 bits (934), Expect = 2.0e-99
Identity = 183/492 (37.20%), Postives = 302/492 (61.38%), Query Frame = 0
Query: 3 MISVCVIIGVVLGVCLLVRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGYSFSSYKTFIV 62
+++ V+ GVVL V +VRR ++ + LG LPPG++GWP++G ++ + F
Sbjct: 19 VLAAAVVAGVVL-VDAVVRRAHDWVRVAALGAERRSRLPPGEMGWPMVGSMWAFLRAF-K 78
Query: 63 QEDPISFIQSLHSRYGKGGMYKTHLYGNPTVIVTNPEICRRIYLDEANFKQHYPKSVKIL 122
+P +FI S R+G+ G+Y+T ++ +PT++ PE C+++ +D+ F +PK+ L
Sbjct: 79 SGNPDAFIASFIRRFGRTGVYRTFMFSSPTILAVTPEACKQVLMDDEGFVTGWPKATVTL 138
Query: 123 EGSSGDFSNM---DHKIAYKVMASPMNGYEVLSNYVDFIEEVIAKGLEEWSSMMREPIKL 182
G F NM DH+ K+ A+P+NG++ L+ Y+ FI++ + L WSS ++
Sbjct: 139 IGPK-SFVNMSYDDHRRIRKLTAAPINGFDALTTYLSFIDQTVVASLRRWSSPESGQVEF 198
Query: 183 VDEIGILFFKVITKIFLGSQLDAKTMVELHTLYKELSFGMVMSTFPYDFPGFTFHQLLKA 242
+ E+ + FK+I +IF+ DA TM L Y +L++G M + PGF +++ L+A
Sbjct: 199 LTELRRMTFKIIVQIFMSGADDA-TMEALERSYTDLNYG--MRAMAINLPGFAYYRALRA 258
Query: 243 RRKIENTIQGVVE-EKIRRFENDKTSEVQCQVDKLVVAINENGAKLYSNNFIRDLILGIF 302
RRK+ + +QGV++ + + K S +D+L+ A +E G +L + + D+++
Sbjct: 259 RRKLVSVLQGVLDGRRAAAAKGFKRSGAMDMMDRLIEAEDERGRRLADDEIV-DVLIMYL 318
Query: 303 FAGHSTPAIAACWALLHISQNPHVFQKAKEEQESIIRQRPSVQKGLTLNEIKQMKYLTKI 362
AGH + WA + + +NP +F +AK EQE I+R P+ Q GLTL + K+M +L+++
Sbjct: 319 NAGHESSGHITMWATVFLQENPDIFARAKAEQEEIMRSIPATQNGLTLRDFKKMHFLSQV 378
Query: 363 INEVLRRNTITATNFRETKTDVNINGHFIPKGWTVQIWNIAVHMDPQIYSNPQEFNPSRW 422
++E LR I+ +FR+ D+ +NG+ IPKGW VQ+W +VHMD Q+Y +P+ FNPSRW
Sbjct: 379 VDETLRCVNISFVSFRQATRDIFVNGYLIPKGWKVQLWYRSVHMDDQVYPDPKMFNPSRW 438
Query: 423 DNYTPKPGEFIPFGLGSRFCPGSELAKLEITILLHHFILNYKMEGVNLESNNNQMPSPRP 482
+ PK G F+PFGLG+R CPG++LAKLEI++ LHHF+L YK++ N + +P PRP
Sbjct: 439 EGPPPKAGTFLPFGLGARLCPGNDLAKLEISVFLHHFLLGYKLKRANPKCRVRYLPHPRP 498
Query: 483 -ENCLCKIIRLS 490
+NCL I ++S
Sbjct: 499 VDNCLATITKVS 503
BLAST of CsGy3G044370 vs. ExPASy Swiss-Prot
Match:
Q9AXH9 (Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1)
HSP 1 Score: 356.7 bits (914), Expect = 4.2e-97
Identity = 184/495 (37.17%), Postives = 296/495 (59.80%), Query Frame = 0
Query: 4 ISVCVIIGVVLGVCLLVRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGYSFSSYKTFIVQ 63
++ V V+ + VR + ++ LG LPPGD+GWP++G ++ + F
Sbjct: 9 VAAVVAALAVVALDAAVRAAHAWYWTASLGAGRRGRLPPGDMGWPLVGGMWAFLRAF-KS 68
Query: 64 EDPISFIQSLHSRYGKGGMYKTHLYGNPTVIVTNPEICRRIYLDEANFKQHYPKSVKILE 123
P SFI S R+G+ G+Y+ ++ +PT++ T PE C+++ +D+ F +PK+ L
Sbjct: 69 GRPDSFIDSFARRFGRAGLYRAFMFSSPTIMATTPEACKQVLMDDDAFVTGWPKATVALI 128
Query: 124 GSSGDFSNM---DHKIAYKVMASPMNGYEVLSNYVDFIEEVIAKGLEEWSSMMRE-PIKL 183
G F NM +H+ K+ A+P+NG++ L++Y+ FI++ + L WS + +
Sbjct: 129 GPK-SFVNMGYDEHRRLRKLTAAPINGFDALTSYLGFIDDTVVTTLRGWSERGGDGHFEF 188
Query: 184 VDEIGILFFKVITKIFLGSQLDAKTMVELHTLYKELSFGMVMSTFPYDFPGFTFHQLLKA 243
+ E+ + F++I +IF+G D +T EL Y EL++G M D PGF +H+ ++A
Sbjct: 189 LTELRRMTFRIIVQIFMGG-ADERTAAELERTYTELNYG--MRAMAIDLPGFAYHKAIRA 248
Query: 244 RRKIENTIQGVVEEKIRRFENDKTSEVQC----QVDKLVVAINENGAKLYSNNFIRDLIL 303
RR++ +Q V++E RR KT+ +D+L+ +E G +L + I D+++
Sbjct: 249 RRRLVAALQRVLDE--RRARGGKTAAGAAAPVDMMDRLIAVEDEGGRRLQDDEII-DVLV 308
Query: 304 GIFFAGHSTPAIAACWALLHISQNPHVFQKAKEEQESIIRQRPSVQKGLTLNEIKQMKYL 363
AGH + WA + + +NP + KAK EQE+I+R P QKGLTL + ++M YL
Sbjct: 309 MYLNAGHESSGHITMWATVFLQENPEILAKAKAEQEAIMRSIPPGQKGLTLRDFRKMAYL 368
Query: 364 TKIINEVLRRNTITATNFRETKTDVNINGHFIPKGWTVQIWNIAVHMDPQIYSNPQEFNP 423
+++++E LR I+ +FR+ DV +NG+ IPKGW VQ+W +VHMDPQ+Y +P++F+P
Sbjct: 369 SQVVDETLRFVNISFVSFRQATRDVFVNGYLIPKGWKVQLWYRSVHMDPQVYPDPKKFDP 428
Query: 424 SRWDNYTPKPGEFIPFGLGSRFCPGSELAKLEITILLHHFILNYKMEGVNLESNNNQMPS 483
SRW+ P+ G F+PFGLG+R CPG++LAKLEI++ LHHF+L YK+ N +P
Sbjct: 429 SRWEGPPPRAGTFLPFGLGTRLCPGNDLAKLEISVFLHHFLLGYKLTRKNPNCRVRYLPH 488
Query: 484 PRP-ENCLCKIIRLS 490
PRP +NCL KI RLS
Sbjct: 489 PRPVDNCLAKITRLS 495
BLAST of CsGy3G044370 vs. ExPASy Swiss-Prot
Match:
O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)
HSP 1 Score: 349.0 bits (894), Expect = 8.9e-95
Identity = 189/475 (39.79%), Postives = 286/475 (60.21%), Query Frame = 0
Query: 15 GVCLLVRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGYSFSSYKTFIVQEDPISFIQSLH 74
G+ LVR++N Y LG + LPPGDLGWP IG S + F DP SF ++L
Sbjct: 21 GLNWLVRKVNVWLYESSLGENRH-YLPPGDLGWPFIGNMLSFLRAF-KTSDPDSFTRTLI 80
Query: 75 SRYGKGGMYKTHLYGNPTVIVTNPEICRRIYLDEANFKQHYPKSVKILEG--SSGDFSNM 134
RYG G+YK H++GNP++IVT + CRR+ D+ FK +P S L G S S
Sbjct: 81 KRYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFE 140
Query: 135 DHKIAYKVMASPMNGYEVLSNYVDFIEEVIAKGLEEWSSMMREPIKLVDEIGILFFKVIT 194
+HK ++ A+P+NG+E LS Y+ +IEE + L++W+ M + + + L F++I
Sbjct: 141 EHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKM--GEFEFLTHLRKLTFRIIM 200
Query: 195 KIFLGSQLDAKTMVELHTLYKELSFGMVMSTFPYDFPGFTFHQLLKARRKIENTIQGVVE 254
IFL S+ + M L Y L++G + + PGF +H+ LKAR+ + Q +V
Sbjct: 201 YIFLSSESE-NVMDALEREYTALNYG--VRAMAVNIPGFAYHRALKARKTLVAAFQSIVT 260
Query: 255 EKIRRFENDKTSEVQCQVDKLVVAINENGAKLYSNNFIRDLILGIFFAGHSTPAIAACWA 314
E+ + + + S + +D L+ +E+G L I D++L AGH + WA
Sbjct: 261 ERRNQRKQNILSNKKDMLDNLLNVKDEDGKTLDDEEII-DVLLMYLNAGHESSGHTIMWA 320
Query: 315 LLHISQNPHVFQKAKEEQESIIRQRPSVQKGLTLNEIKQMKYLTKIINEVLRRNTITATN 374
+ + ++P V Q+AK EQE I++ RP QKGL+L E ++M++L+++++E LR T + T
Sbjct: 321 TVFLQEHPEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVITFSLTA 380
Query: 375 FRETKTDVNINGHFIPKGWTVQIWNIAVHMDPQIYSNPQEFNPSRWDN-YTPKPGEFIPF 434
FRE KTDV +NG+ IPKGW V W VH+DP+++ +P++F+P+RWDN + PK G F+PF
Sbjct: 381 FREAKTDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKAGAFLPF 440
Query: 435 GLGSRFCPGSELAKLEITILLHHFILNYKMEGVNLESNNNQMPSPRP-ENCLCKI 486
G GS CPG++LAKLEI+I LHHF+L Y+++ N E +P RP +NCL +I
Sbjct: 441 GAGSHLCPGNDLAKLEISIFLHHFLLKYQVKRSNPECPVMYLPHTRPTDNCLARI 487
BLAST of CsGy3G044370 vs. NCBI nr
Match:
XP_004148515.2 (beta-amyrin 11-oxidase [Cucumis sativus] >AIT72036.1 cytochrome P450 [Cucumis sativus] >KGN60415.1 hypothetical protein Csa_001840 [Cucumis sativus])
HSP 1 Score: 996 bits (2574), Expect = 0.0
Identity = 489/489 (100.00%), Postives = 489/489 (100.00%), Query Frame = 0
Query: 1 MEMISVCVIIGVVLGVCLLVRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGYSFSSYKTF 60
MEMISVCVIIGVVLGVCLLVRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGYSFSSYKTF
Sbjct: 1 MEMISVCVIIGVVLGVCLLVRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGYSFSSYKTF 60
Query: 61 IVQEDPISFIQSLHSRYGKGGMYKTHLYGNPTVIVTNPEICRRIYLDEANFKQHYPKSVK 120
IVQEDPISFIQSLHSRYGKGGMYKTHLYGNPTVIVTNPEICRRIYLDEANFKQHYPKSVK
Sbjct: 61 IVQEDPISFIQSLHSRYGKGGMYKTHLYGNPTVIVTNPEICRRIYLDEANFKQHYPKSVK 120
Query: 121 ILEGSSGDFSNMDHKIAYKVMASPMNGYEVLSNYVDFIEEVIAKGLEEWSSMMREPIKLV 180
ILEGSSGDFSNMDHKIAYKVMASPMNGYEVLSNYVDFIEEVIAKGLEEWSSMMREPIKLV
Sbjct: 121 ILEGSSGDFSNMDHKIAYKVMASPMNGYEVLSNYVDFIEEVIAKGLEEWSSMMREPIKLV 180
Query: 181 DEIGILFFKVITKIFLGSQLDAKTMVELHTLYKELSFGMVMSTFPYDFPGFTFHQLLKAR 240
DEIGILFFKVITKIFLGSQLDAKTMVELHTLYKELSFGMVMSTFPYDFPGFTFHQLLKAR
Sbjct: 181 DEIGILFFKVITKIFLGSQLDAKTMVELHTLYKELSFGMVMSTFPYDFPGFTFHQLLKAR 240
Query: 241 RKIENTIQGVVEEKIRRFENDKTSEVQCQVDKLVVAINENGAKLYSNNFIRDLILGIFFA 300
RKIENTIQGVVEEKIRRFENDKTSEVQCQVDKLVVAINENGAKLYSNNFIRDLILGIFFA
Sbjct: 241 RKIENTIQGVVEEKIRRFENDKTSEVQCQVDKLVVAINENGAKLYSNNFIRDLILGIFFA 300
Query: 301 GHSTPAIAACWALLHISQNPHVFQKAKEEQESIIRQRPSVQKGLTLNEIKQMKYLTKIIN 360
GHSTPAIAACWALLHISQNPHVFQKAKEEQESIIRQRPSVQKGLTLNEIKQMKYLTKIIN
Sbjct: 301 GHSTPAIAACWALLHISQNPHVFQKAKEEQESIIRQRPSVQKGLTLNEIKQMKYLTKIIN 360
Query: 361 EVLRRNTITATNFRETKTDVNINGHFIPKGWTVQIWNIAVHMDPQIYSNPQEFNPSRWDN 420
EVLRRNTITATNFRETKTDVNINGHFIPKGWTVQIWNIAVHMDPQIYSNPQEFNPSRWDN
Sbjct: 361 EVLRRNTITATNFRETKTDVNINGHFIPKGWTVQIWNIAVHMDPQIYSNPQEFNPSRWDN 420
Query: 421 YTPKPGEFIPFGLGSRFCPGSELAKLEITILLHHFILNYKMEGVNLESNNNQMPSPRPEN 480
YTPKPGEFIPFGLGSRFCPGSELAKLEITILLHHFILNYKMEGVNLESNNNQMPSPRPEN
Sbjct: 421 YTPKPGEFIPFGLGSRFCPGSELAKLEITILLHHFILNYKMEGVNLESNNNQMPSPRPEN 480
Query: 481 CLCKIIRLS 489
CLCKIIRLS
Sbjct: 481 CLCKIIRLS 489
BLAST of CsGy3G044370 vs. NCBI nr
Match:
XP_008466645.1 (PREDICTED: beta-amyrin 11-oxidase-like, partial [Cucumis melo])
HSP 1 Score: 834 bits (2155), Expect = 3.82e-303
Identity = 410/459 (89.32%), Postives = 434/459 (94.55%), Query Frame = 0
Query: 1 MEMISVCVIIGVVLGVCLLV-RRLNELWYLVKLGGRAYKSLPPGDLGWPVIGYSFSSYKT 60
MEMISVCV IGVVLGV LLV RRLNELWYLVKLGG+AYKSLPPGDLGWPVIG SFSSYKT
Sbjct: 1 MEMISVCVTIGVVLGVSLLVFRRLNELWYLVKLGGKAYKSLPPGDLGWPVIGSSFSSYKT 60
Query: 61 FIVQEDPISFIQSLHSRYGKGGMYKTHLYGNPTVIVTNPEICRRIYLDEANFKQHYPKSV 120
F+VQEDPISFIQSLHSRYG+GG+YKTHLYG P VI T+ EICRRIYLDEA FKQH P+SV
Sbjct: 61 FMVQEDPISFIQSLHSRYGEGGIYKTHLYGKPIVIATDSEICRRIYLDEAKFKQHNPESV 120
Query: 121 KILEGSSGDFSNMDHKIAYKVMASPMNGYEVLSNYVDFIEEVIAKGLEEWSSMMREPIKL 180
KILEGSSGDFS MDHKIAYKV+A+PMNG EVLSNYVDFIEEVIAKGLEEWSSM+REPIKL
Sbjct: 121 KILEGSSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKL 180
Query: 181 VDEIGILFFKVITKIFLGSQLDAKTMVELHTLYKELSFGMVMSTFPYDFPGFTFHQLLKA 240
+DEIG LFFKVITK+FLGS+LDAKTMVELH LYKELSF MVMSTFPYDFPGFTFHQ LKA
Sbjct: 181 LDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQALKA 240
Query: 241 RRKIENTIQGVVEEKIRRFENDKTSEVQCQVDKLVVAINENGAKLYSNNFIRDLILGIFF 300
R+KIEN IQGVVEEK RRFENDKTSEVQCQVDK+VVAI+ENGAKLY+NN IRDL+LG+ F
Sbjct: 241 RKKIENIIQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNNNLIRDLLLGVLF 300
Query: 301 AGHSTPAIAACWALLHISQNPHVFQKAKEEQESIIRQRPSVQKGLTLNEIKQMKYLTKII 360
AGHSTPAIAA WALLHISQNPHVFQKAKEEQESIIRQRPS QKGLTLNEIKQMKYLTK+I
Sbjct: 301 AGHSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYLTKVI 360
Query: 361 NEVLRRNTITATNFRETKTDVNINGHFIPKGWTVQIWNIAVHMDPQIYSNPQEFNPSRWD 420
NEVLRRNTIT TNFRE KTDVNING+FIPKGWT+QIWNIAVHMDPQI+SNPQEFNPSRW+
Sbjct: 361 NEVLRRNTITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIHSNPQEFNPSRWE 420
Query: 421 NYTPKPGEFIPFGLGSRFCPGSELAKLEITILLHHFILN 458
NYTPKPG FIPFGLGSRFCPGSELA+LEITILLHHFILN
Sbjct: 421 NYTPKPGTFIPFGLGSRFCPGSELARLEITILLHHFILN 459
BLAST of CsGy3G044370 vs. NCBI nr
Match:
XP_011652660.1 (beta-amyrin 11-oxidase [Cucumis sativus])
HSP 1 Score: 709 bits (1831), Expect = 2.56e-253
Identity = 349/492 (70.93%), Postives = 409/492 (83.13%), Query Frame = 0
Query: 1 MEMISVCVIIGVVLGVCLLVRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGYSFSSYKTF 60
ME+++VCVIIGVVLG + VRR NE+WYL KLG + KSLPPGDLGWP +G +FS YK F
Sbjct: 1 MELMNVCVIIGVVLGFYVFVRRFNEIWYLFKLGRKLNKSLPPGDLGWPFVGSTFSFYKAF 60
Query: 61 IVQEDPISFIQSLHSRYGKGGMYKTHLYGNPTVIVTNPEICRRIYLDEANFKQHYPKSVK 120
V+ DP +FI +L RYG+ GMYK+HLYG PT+IVTNPEICRRIYLD+ F+ +YPKSVK
Sbjct: 61 KVEGDPYTFIHTLLLRYGRVGMYKSHLYGMPTIIVTNPEICRRIYLDDERFEPNYPKSVK 120
Query: 121 ILEGSSGDFSNMDHKIAYKVMASPMNGYEVLSNYVDFIEEVIAKGLEEWSSMMREPIKLV 180
ILE ++GDFS +DHK YK+MASPMNG EVLS +V+FIE+ + KGLEEWSSM REPI+LV
Sbjct: 121 ILE-TNGDFSKIDHKSGYKIMASPMNGSEVLSKHVEFIEQTVEKGLEEWSSMRREPIELV 180
Query: 181 DEIGILFFKVITKIFLGSQLDAKTMVELHTLYKELSFGMVMSTFPYDFPGFTFHQLLKAR 240
DEIG L FK+I IFLG+++D + M ELHTLYKEL ++MS PYD PGFT+ + LKAR
Sbjct: 181 DEIGGLLFKIILHIFLGNEIDGQAMAELHTLYKELGL-VIMSFLPYDLPGFTYRRALKAR 240
Query: 241 RKIENTIQGVVEEKIRRFENDK-TSEV-QCQVDKLVVAINENGAKLYSNNFIRDLILGIF 300
+KIE + V+E+K +RFE D T+EV CQVDKL+VA NENG+K Y+N+ I DLILGIF
Sbjct: 241 KKIEKILHCVIEKKRKRFEKDDGTNEVVHCQVDKLIVATNENGSKPYNNSTIIDLILGIF 300
Query: 301 FAGHSTPAIAACWALLHISQNPHVFQKAKEEQESIIRQRPSVQKGLTLNEIKQMKYLTKI 360
FAGH+TPAIAA WALLHISQNPH+FQ AKEEQESIIRQRPS QKGLT EIKQMKYLTK
Sbjct: 301 FAGHNTPAIAAMWALLHISQNPHIFQMAKEEQESIIRQRPSTQKGLTFQEIKQMKYLTKF 360
Query: 361 INEVLRRNTITATNFRETKTDVNINGHFIPKGWTVQIWNIAVHMDPQIYSNPQEFNPSRW 420
INEVLRRNT+ TNFR+ +T VNING+ IPKGWTVQIW++A+HMD QIYSN QEF+PSRW
Sbjct: 361 INEVLRRNTVAPTNFRKARTYVNINGYTIPKGWTVQIWSVAIHMDSQIYSNSQEFDPSRW 420
Query: 421 DNYTPKPGEFIPFGLGSRFCPGSELAKLEITILLHHFILNYKMEGVNLESNNNQMPSPRP 480
DNYTPKPGEFIPFGLGSRFCPGSELAKLEITILLHHFILNYKME V+ +PSP+P
Sbjct: 421 DNYTPKPGEFIPFGLGSRFCPGSELAKLEITILLHHFILNYKMELVDQNCKVTHLPSPKP 480
Query: 481 -ENCLCKIIRLS 489
+NCLCKIIR+S
Sbjct: 481 RDNCLCKIIRVS 490
BLAST of CsGy3G044370 vs. NCBI nr
Match:
KAE8651450.1 (hypothetical protein Csa_001467 [Cucumis sativus])
HSP 1 Score: 691 bits (1783), Expect = 8.84e-238
Identity = 338/478 (70.71%), Postives = 395/478 (82.64%), Query Frame = 0
Query: 15 GVCLLVRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGYSFSSYKTFIVQEDPISFIQSLH 74
G + VRR NE+WYL KLG + KSLPPGDLGWP +G +FS YK F V+ DP +FI +L
Sbjct: 581 GFYVFVRRFNEIWYLFKLGRKLNKSLPPGDLGWPFVGSTFSFYKAFKVEGDPYTFIHTLL 640
Query: 75 SRYGKGGMYKTHLYGNPTVIVTNPEICRRIYLDEANFKQHYPKSVKILEGSSGDFSNMDH 134
RYG+ GMYK+HLYG PT+IVTNPEICRRIYLD+ F+ +YPKSVKILE ++GDFS +DH
Sbjct: 641 LRYGRVGMYKSHLYGMPTIIVTNPEICRRIYLDDERFEPNYPKSVKILE-TNGDFSKIDH 700
Query: 135 KIAYKVMASPMNGYEVLSNYVDFIEEVIAKGLEEWSSMMREPIKLVDEIGILFFKVITKI 194
K YK+MASPMNG EVLS +V+FIE+ + KGLEEWSSM REPI+LVDEIG L FK+I I
Sbjct: 701 KSGYKIMASPMNGSEVLSKHVEFIEQTVEKGLEEWSSMRREPIELVDEIGGLLFKIILHI 760
Query: 195 FLGSQLDAKTMVELHTLYKELSFGMVMSTFPYDFPGFTFHQLLKARRKIENTIQGVVEEK 254
FLG+++D + M ELHTLYKEL ++MS PYD PGFT+ + LKAR+KIE + V+E+K
Sbjct: 761 FLGNEIDGQAMAELHTLYKELGL-VIMSFLPYDLPGFTYRRALKARKKIEKILHCVIEKK 820
Query: 255 IRRFENDK-TSEV-QCQVDKLVVAINENGAKLYSNNFIRDLILGIFFAGHSTPAIAACWA 314
+RFE D T+EV CQVDKL+VA NENG+K Y+N+ I DLILGIFFAGH+TPAIAA WA
Sbjct: 821 RKRFEKDDGTNEVVHCQVDKLIVATNENGSKPYNNSTIIDLILGIFFAGHNTPAIAAMWA 880
Query: 315 LLHISQNPHVFQKAKEEQESIIRQRPSVQKGLTLNEIKQMKYLTKIINEVLRRNTITATN 374
LLHISQNPH+FQ AKEEQESIIRQRPS QKGLT EIKQMKYLTK INEVLRRNT+ TN
Sbjct: 881 LLHISQNPHIFQMAKEEQESIIRQRPSTQKGLTFQEIKQMKYLTKFINEVLRRNTVAPTN 940
Query: 375 FRETKTDVNINGHFIPKGWTVQIWNIAVHMDPQIYSNPQEFNPSRWDNYTPKPGEFIPFG 434
FR+ +T VNING+ IPKGWTVQIW++A+HMD QIYSN QEF+PSRWDNYTPKPGEFIPFG
Sbjct: 941 FRKARTYVNINGYTIPKGWTVQIWSVAIHMDSQIYSNSQEFDPSRWDNYTPKPGEFIPFG 1000
Query: 435 LGSRFCPGSELAKLEITILLHHFILNYKMEGVNLESNNNQMPSPRP-ENCLCKIIRLS 489
LGSRFCPGSELAKLEITILLHHFILNYKME V+ +PSP+P +NCLCKIIR+S
Sbjct: 1001 LGSRFCPGSELAKLEITILLHHFILNYKMELVDQNCKVTHLPSPKPRDNCLCKIIRVS 1056
BLAST of CsGy3G044370 vs. NCBI nr
Match:
XP_022952147.1 (beta-amyrin 11-oxidase-like [Cucurbita moschata])
HSP 1 Score: 662 bits (1707), Expect = 1.66e-234
Identity = 321/489 (65.64%), Postives = 393/489 (80.37%), Query Frame = 0
Query: 1 MEMISVCVIIGVVLGVCLLVRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGYSFSSYKTF 60
MEMI+VC IIG VLGV + VR+LNE+WYLVKLG +AYKSLPPGD+GWP++G SFS Y F
Sbjct: 1 MEMINVCTIIGAVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAF 60
Query: 61 IVQEDPISFIQSLHSRYGKGGMYKTHLYGNPTVIVTNPEICRRIYLDEANFKQHYPKSVK 120
V DP SFI L SR G+ GMYK+HLYG PT+IVT+PEICRRIYLD+ FK +YPKSVK
Sbjct: 61 TVGGDPDSFIYHLLSRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDEIFKPNYPKSVK 120
Query: 121 ILEGSSGDFSNMDHKIAYKVMASPMNGYEVLSNYVDFIEEVIAKGLEEWSSMMREPIKLV 180
ILE +G F+ DHK AY++MA+PMNGYEVLS+YVDFI++V+AKGLEEWSSM +EPI+L+
Sbjct: 121 ILE-VNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSM-KEPIELL 180
Query: 181 DEIGILFFKVITKIFLGSQLDAKTMVELHTLYKELSFGMVMSTFPYDFPGFTFHQLLKAR 240
EIG LFFKVI++IFLG+ + A M EL TLYK+L ++S PYD PG +H+ LKAR
Sbjct: 181 GEIGSLFFKVISRIFLGTDIGASRMAELETLYKDLG-PAILSILPYDVPGLVYHRALKAR 240
Query: 241 RKIENTIQGVVEEKIRRFENDKTSEVQCQVDKLVVAINENGAKLYSNNFIRDLILGIFFA 300
+KIEN ++ V+EEK R E +T EV Q+DKL++A +ENG KLY NN I DL+LG+ A
Sbjct: 241 KKIENVLECVIEEKRRALEQKETREVHSQMDKLIMARDENGMKLYDNNTIIDLLLGLLHA 300
Query: 301 GHSTPAIAACWALLHISQNPHVFQKAKEEQESIIRQRPSVQKGLTLNEIKQMKYLTKIIN 360
GH TPA AA WAL+HIS+ PH+F KAKEEQESI++QRPS QKGLT EIKQMKYL K IN
Sbjct: 301 GHHTPAYAAMWALVHISEKPHIFHKAKEEQESILKQRPSTQKGLTFQEIKQMKYLMKFIN 360
Query: 361 EVLRRNTITATNFRETKTDVNINGHFIPKGWTVQIWNIAVHMDPQIYSNPQEFNPSRWDN 420
E+LRRNTI +FR+ KTD+NIN + IPKGW+V IW AVHMDPQ+YSNP+EF+PSRW+N
Sbjct: 361 ELLRRNTIVVASFRKAKTDININDYIIPKGWSVLIWTRAVHMDPQLYSNPEEFDPSRWEN 420
Query: 421 YTPKPGEFIPFGLGSRFCPGSELAKLEITILLHHFILNYKMEGVNLESNNNQMPSPR-PE 480
++PKPG FIPFG+G+RFCPGSEL KLEITILLHHFILNYKME N + + +P+P+ +
Sbjct: 421 HSPKPGTFIPFGIGTRFCPGSELTKLEITILLHHFILNYKMERTNPKCCISHLPAPKLVD 480
Query: 481 NCLCKIIRL 488
NCLC I +L
Sbjct: 481 NCLCTITKL 486
BLAST of CsGy3G044370 vs. ExPASy TrEMBL
Match:
A0A097IYP6 (Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa3G903550 PE=2 SV=1)
HSP 1 Score: 996 bits (2574), Expect = 0.0
Identity = 489/489 (100.00%), Postives = 489/489 (100.00%), Query Frame = 0
Query: 1 MEMISVCVIIGVVLGVCLLVRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGYSFSSYKTF 60
MEMISVCVIIGVVLGVCLLVRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGYSFSSYKTF
Sbjct: 1 MEMISVCVIIGVVLGVCLLVRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGYSFSSYKTF 60
Query: 61 IVQEDPISFIQSLHSRYGKGGMYKTHLYGNPTVIVTNPEICRRIYLDEANFKQHYPKSVK 120
IVQEDPISFIQSLHSRYGKGGMYKTHLYGNPTVIVTNPEICRRIYLDEANFKQHYPKSVK
Sbjct: 61 IVQEDPISFIQSLHSRYGKGGMYKTHLYGNPTVIVTNPEICRRIYLDEANFKQHYPKSVK 120
Query: 121 ILEGSSGDFSNMDHKIAYKVMASPMNGYEVLSNYVDFIEEVIAKGLEEWSSMMREPIKLV 180
ILEGSSGDFSNMDHKIAYKVMASPMNGYEVLSNYVDFIEEVIAKGLEEWSSMMREPIKLV
Sbjct: 121 ILEGSSGDFSNMDHKIAYKVMASPMNGYEVLSNYVDFIEEVIAKGLEEWSSMMREPIKLV 180
Query: 181 DEIGILFFKVITKIFLGSQLDAKTMVELHTLYKELSFGMVMSTFPYDFPGFTFHQLLKAR 240
DEIGILFFKVITKIFLGSQLDAKTMVELHTLYKELSFGMVMSTFPYDFPGFTFHQLLKAR
Sbjct: 181 DEIGILFFKVITKIFLGSQLDAKTMVELHTLYKELSFGMVMSTFPYDFPGFTFHQLLKAR 240
Query: 241 RKIENTIQGVVEEKIRRFENDKTSEVQCQVDKLVVAINENGAKLYSNNFIRDLILGIFFA 300
RKIENTIQGVVEEKIRRFENDKTSEVQCQVDKLVVAINENGAKLYSNNFIRDLILGIFFA
Sbjct: 241 RKIENTIQGVVEEKIRRFENDKTSEVQCQVDKLVVAINENGAKLYSNNFIRDLILGIFFA 300
Query: 301 GHSTPAIAACWALLHISQNPHVFQKAKEEQESIIRQRPSVQKGLTLNEIKQMKYLTKIIN 360
GHSTPAIAACWALLHISQNPHVFQKAKEEQESIIRQRPSVQKGLTLNEIKQMKYLTKIIN
Sbjct: 301 GHSTPAIAACWALLHISQNPHVFQKAKEEQESIIRQRPSVQKGLTLNEIKQMKYLTKIIN 360
Query: 361 EVLRRNTITATNFRETKTDVNINGHFIPKGWTVQIWNIAVHMDPQIYSNPQEFNPSRWDN 420
EVLRRNTITATNFRETKTDVNINGHFIPKGWTVQIWNIAVHMDPQIYSNPQEFNPSRWDN
Sbjct: 361 EVLRRNTITATNFRETKTDVNINGHFIPKGWTVQIWNIAVHMDPQIYSNPQEFNPSRWDN 420
Query: 421 YTPKPGEFIPFGLGSRFCPGSELAKLEITILLHHFILNYKMEGVNLESNNNQMPSPRPEN 480
YTPKPGEFIPFGLGSRFCPGSELAKLEITILLHHFILNYKMEGVNLESNNNQMPSPRPEN
Sbjct: 421 YTPKPGEFIPFGLGSRFCPGSELAKLEITILLHHFILNYKMEGVNLESNNNQMPSPRPEN 480
Query: 481 CLCKIIRLS 489
CLCKIIRLS
Sbjct: 481 CLCKIIRLS 489
BLAST of CsGy3G044370 vs. ExPASy TrEMBL
Match:
A0A1S3CRX8 (beta-amyrin 11-oxidase-like OS=Cucumis melo OX=3656 GN=LOC103504002 PE=3 SV=1)
HSP 1 Score: 834 bits (2155), Expect = 1.85e-303
Identity = 410/459 (89.32%), Postives = 434/459 (94.55%), Query Frame = 0
Query: 1 MEMISVCVIIGVVLGVCLLV-RRLNELWYLVKLGGRAYKSLPPGDLGWPVIGYSFSSYKT 60
MEMISVCV IGVVLGV LLV RRLNELWYLVKLGG+AYKSLPPGDLGWPVIG SFSSYKT
Sbjct: 1 MEMISVCVTIGVVLGVSLLVFRRLNELWYLVKLGGKAYKSLPPGDLGWPVIGSSFSSYKT 60
Query: 61 FIVQEDPISFIQSLHSRYGKGGMYKTHLYGNPTVIVTNPEICRRIYLDEANFKQHYPKSV 120
F+VQEDPISFIQSLHSRYG+GG+YKTHLYG P VI T+ EICRRIYLDEA FKQH P+SV
Sbjct: 61 FMVQEDPISFIQSLHSRYGEGGIYKTHLYGKPIVIATDSEICRRIYLDEAKFKQHNPESV 120
Query: 121 KILEGSSGDFSNMDHKIAYKVMASPMNGYEVLSNYVDFIEEVIAKGLEEWSSMMREPIKL 180
KILEGSSGDFS MDHKIAYKV+A+PMNG EVLSNYVDFIEEVIAKGLEEWSSM+REPIKL
Sbjct: 121 KILEGSSGDFSKMDHKIAYKVVATPMNGSEVLSNYVDFIEEVIAKGLEEWSSMLREPIKL 180
Query: 181 VDEIGILFFKVITKIFLGSQLDAKTMVELHTLYKELSFGMVMSTFPYDFPGFTFHQLLKA 240
+DEIG LFFKVITK+FLGS+LDAKTMVELH LYKELSF MVMSTFPYDFPGFTFHQ LKA
Sbjct: 181 LDEIGSLFFKVITKVFLGSRLDAKTMVELHALYKELSFAMVMSTFPYDFPGFTFHQALKA 240
Query: 241 RRKIENTIQGVVEEKIRRFENDKTSEVQCQVDKLVVAINENGAKLYSNNFIRDLILGIFF 300
R+KIEN IQGVVEEK RRFENDKTSEVQCQVDK+VVAI+ENGAKLY+NN IRDL+LG+ F
Sbjct: 241 RKKIENIIQGVVEEKRRRFENDKTSEVQCQVDKMVVAIDENGAKLYNNNLIRDLLLGVLF 300
Query: 301 AGHSTPAIAACWALLHISQNPHVFQKAKEEQESIIRQRPSVQKGLTLNEIKQMKYLTKII 360
AGHSTPAIAA WALLHISQNPHVFQKAKEEQESIIRQRPS QKGLTLNEIKQMKYLTK+I
Sbjct: 301 AGHSTPAIAAFWALLHISQNPHVFQKAKEEQESIIRQRPSNQKGLTLNEIKQMKYLTKVI 360
Query: 361 NEVLRRNTITATNFRETKTDVNINGHFIPKGWTVQIWNIAVHMDPQIYSNPQEFNPSRWD 420
NEVLRRNTIT TNFRE KTDVNING+FIPKGWT+QIWNIAVHMDPQI+SNPQEFNPSRW+
Sbjct: 361 NEVLRRNTITPTNFREAKTDVNINGYFIPKGWTIQIWNIAVHMDPQIHSNPQEFNPSRWE 420
Query: 421 NYTPKPGEFIPFGLGSRFCPGSELAKLEITILLHHFILN 458
NYTPKPG FIPFGLGSRFCPGSELA+LEITILLHHFILN
Sbjct: 421 NYTPKPGTFIPFGLGSRFCPGSELARLEITILLHHFILN 459
BLAST of CsGy3G044370 vs. ExPASy TrEMBL
Match:
A0A6J1GJM1 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454910 PE=3 SV=1)
HSP 1 Score: 662 bits (1707), Expect = 8.02e-235
Identity = 321/489 (65.64%), Postives = 393/489 (80.37%), Query Frame = 0
Query: 1 MEMISVCVIIGVVLGVCLLVRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGYSFSSYKTF 60
MEMI+VC IIG VLGV + VR+LNE+WYLVKLG +AYKSLPPGD+GWP++G SFS Y F
Sbjct: 1 MEMINVCTIIGAVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAF 60
Query: 61 IVQEDPISFIQSLHSRYGKGGMYKTHLYGNPTVIVTNPEICRRIYLDEANFKQHYPKSVK 120
V DP SFI L SR G+ GMYK+HLYG PT+IVT+PEICRRIYLD+ FK +YPKSVK
Sbjct: 61 TVGGDPDSFIYHLLSRNGRVGMYKSHLYGRPTIIVTDPEICRRIYLDDEIFKPNYPKSVK 120
Query: 121 ILEGSSGDFSNMDHKIAYKVMASPMNGYEVLSNYVDFIEEVIAKGLEEWSSMMREPIKLV 180
ILE +G F+ DHK AY++MA+PMNGYEVLS+YVDFI++V+AKGLEEWSSM +EPI+L+
Sbjct: 121 ILE-VNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSM-KEPIELL 180
Query: 181 DEIGILFFKVITKIFLGSQLDAKTMVELHTLYKELSFGMVMSTFPYDFPGFTFHQLLKAR 240
EIG LFFKVI++IFLG+ + A M EL TLYK+L ++S PYD PG +H+ LKAR
Sbjct: 181 GEIGSLFFKVISRIFLGTDIGASRMAELETLYKDLG-PAILSILPYDVPGLVYHRALKAR 240
Query: 241 RKIENTIQGVVEEKIRRFENDKTSEVQCQVDKLVVAINENGAKLYSNNFIRDLILGIFFA 300
+KIEN ++ V+EEK R E +T EV Q+DKL++A +ENG KLY NN I DL+LG+ A
Sbjct: 241 KKIENVLECVIEEKRRALEQKETREVHSQMDKLIMARDENGMKLYDNNTIIDLLLGLLHA 300
Query: 301 GHSTPAIAACWALLHISQNPHVFQKAKEEQESIIRQRPSVQKGLTLNEIKQMKYLTKIIN 360
GH TPA AA WAL+HIS+ PH+F KAKEEQESI++QRPS QKGLT EIKQMKYL K IN
Sbjct: 301 GHHTPAYAAMWALVHISEKPHIFHKAKEEQESILKQRPSTQKGLTFQEIKQMKYLMKFIN 360
Query: 361 EVLRRNTITATNFRETKTDVNINGHFIPKGWTVQIWNIAVHMDPQIYSNPQEFNPSRWDN 420
E+LRRNTI +FR+ KTD+NIN + IPKGW+V IW AVHMDPQ+YSNP+EF+PSRW+N
Sbjct: 361 ELLRRNTIVVASFRKAKTDININDYIIPKGWSVLIWTRAVHMDPQLYSNPEEFDPSRWEN 420
Query: 421 YTPKPGEFIPFGLGSRFCPGSELAKLEITILLHHFILNYKMEGVNLESNNNQMPSPR-PE 480
++PKPG FIPFG+G+RFCPGSEL KLEITILLHHFILNYKME N + + +P+P+ +
Sbjct: 421 HSPKPGTFIPFGIGTRFCPGSELTKLEITILLHHFILNYKMERTNPKCCISHLPAPKLVD 480
Query: 481 NCLCKIIRL 488
NCLC I +L
Sbjct: 481 NCLCTITKL 486
BLAST of CsGy3G044370 vs. ExPASy TrEMBL
Match:
A0A6J1GLR1 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111455409 PE=3 SV=1)
HSP 1 Score: 659 bits (1701), Expect = 6.32e-234
Identity = 325/489 (66.46%), Postives = 391/489 (79.96%), Query Frame = 0
Query: 1 MEMISVCVIIGVVLGVCLLVRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGYSFSSYKTF 60
ME I+VCVIIG VLGV + VR+LNE+WYLVKLG +AYKSLPPGD+GWP++G SFS Y F
Sbjct: 1 METINVCVIIGFVLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYNAF 60
Query: 61 IVQEDPISFIQSLHSRYGKGGMYKTHLYGNPTVIVTNPEICRRIYLDEANFKQHYPKSVK 120
V DP SFI L SR G+ GMYK+H YG PT+IVT+PEICRRIYLD+ FK +YPKSVK
Sbjct: 61 TVGGDPDSFIYHLLSRNGRVGMYKSHFYGRPTIIVTDPEICRRIYLDDEIFKPNYPKSVK 120
Query: 121 ILEGSSGDFSNMDHKIAYKVMASPMNGYEVLSNYVDFIEEVIAKGLEEWSSMMREPIKLV 180
ILE +G F+ DHK AY++MA+PMNGYEVLS+YVDFI++V+AKGLEEWSSM +EPI+L+
Sbjct: 121 ILE-VNGFFTRADHKSAYRIMAAPMNGYEVLSHYVDFIDQVMAKGLEEWSSM-KEPIELL 180
Query: 181 DEIGILFFKVITKIFLGSQLDAKTMVELHTLYKELSFGMVMSTFPYDFPGFTFHQLLKAR 240
EIG LFFKVI++IFLG+ + A M EL TLYK+L ++S PYD PG TFH LKAR
Sbjct: 181 GEIGSLFFKVISRIFLGTDIGASRMAELETLYKDLG-PAILSILPYDLPGLTFHGALKAR 240
Query: 241 RKIENTIQGVVEEKIRRFENDKTSEVQCQVDKLVVAINENGAKLYSNNFIRDLILGIFFA 300
+KIEN +Q V+EEK R E ++ EV+ Q+DKL++A +ENG KLY NN I DL+LG+ A
Sbjct: 241 KKIENILQCVIEEKRRALEKEER-EVESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHA 300
Query: 301 GHSTPAIAACWALLHISQNPHVFQKAKEEQESIIRQRPSVQKGLTLNEIKQMKYLTKIIN 360
GH TPA AA WAL+HIS+ PHVF KAKEEQESII+QRPS QKGLT EIKQMKYL K IN
Sbjct: 301 GHHTPAYAAMWALVHISEKPHVFHKAKEEQESIIKQRPSTQKGLTFQEIKQMKYLMKFIN 360
Query: 361 EVLRRNTITATNFRETKTDVNINGHFIPKGWTVQIWNIAVHMDPQIYSNPQEFNPSRWDN 420
E+LRRNTI +FR+ K DVNIN + IPKGW V IW A+HMDPQ+YSNPQEF+PSRW+N
Sbjct: 361 ELLRRNTIAVASFRKAKKDVNINDYIIPKGWNVLIWTRAIHMDPQLYSNPQEFDPSRWEN 420
Query: 421 YTPKPGEFIPFGLGSRFCPGSELAKLEITILLHHFILNYKMEGVNLESNNNQMPSPR-PE 480
Y+PKPG F+PFG+G+RFCPGSEL KLEITILLHHFILNYKME VN + +P+P+ +
Sbjct: 421 YSPKPGTFLPFGIGTRFCPGSELTKLEITILLHHFILNYKMERVNPKCCITHLPAPKLVD 480
Query: 481 NCLCKIIRL 488
NCLC I +L
Sbjct: 481 NCLCTITKL 485
BLAST of CsGy3G044370 vs. ExPASy TrEMBL
Match:
A0A6J1GJP8 (beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454912 PE=3 SV=1)
HSP 1 Score: 653 bits (1684), Expect = 2.43e-231
Identity = 318/489 (65.03%), Postives = 393/489 (80.37%), Query Frame = 0
Query: 1 MEMISVCVIIGVVLGVCLLVRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGYSFSSYKTF 60
ME I+VC+IIG +LGV + VR+LNE+WYLVKLG +AYKSLPPGD+GWP++G SFS YK F
Sbjct: 1 METINVCIIIGFLLGVYMFVRKLNEIWYLVKLGRKAYKSLPPGDMGWPLLGSSFSFYKAF 60
Query: 61 IVQEDPISFIQSLHSRYGKGGMYKTHLYGNPTVIVTNPEICRRIYLDEANFKQHYPKSVK 120
IV DP SFI L SR G+ GMYK+HLYG+PT+IVT+PEICRRIYLD+ FK +YPKSVK
Sbjct: 61 IVGGDPDSFIYHLLSRNGRVGMYKSHLYGSPTIIVTDPEICRRIYLDDEIFKPNYPKSVK 120
Query: 121 ILEGSSGDFSNMDHKIAYKVMASPMNGYEVLSNYVDFIEEVIAKGLEEWSSMMREPIKLV 180
ILE +G FS MDHK AY++MA+P+NGY VLS+YVDFI++++AKGLEEWS+ +EPI+L
Sbjct: 121 ILE-VNGLFSRMDHKCAYRIMAAPINGYNVLSHYVDFIDQIMAKGLEEWSTK-KEPIELF 180
Query: 181 DEIGILFFKVITKIFLGSQLDAKTMVELHTLYKELSFGMVMSTFPYDFPGFTFHQLLKAR 240
EIG LFF+VI++IFLG+ + A M EL TLYK+L ++S PYD PG TFH+ LKAR
Sbjct: 181 GEIGSLFFRVISRIFLGTDIGASRMAELETLYKDLG-PAILSILPYDLPGLTFHRALKAR 240
Query: 241 RKIENTIQGVVEEKIRRFENDKTSEVQCQVDKLVVAINENGAKLYSNNFIRDLILGIFFA 300
+KIEN +Q V+EEK R E + EV+ Q+DKL++A +ENG KLY NN I DL+LG+
Sbjct: 241 KKIENILQCVIEEKRRALEKQER-EVESQMDKLIMARDENGMKLYDNNAIIDLLLGLLHG 300
Query: 301 GHSTPAIAACWALLHISQNPHVFQKAKEEQESIIRQRPSVQKGLTLNEIKQMKYLTKIIN 360
GH TPA AA WAL+HIS+ PHVF KAKEEQESI++QR S QKGLT EIKQMKYL K IN
Sbjct: 301 GHHTPAYAAMWALVHISETPHVFHKAKEEQESILKQRSSTQKGLTFQEIKQMKYLMKFIN 360
Query: 361 EVLRRNTITATNFRETKTDVNINGHFIPKGWTVQIWNIAVHMDPQIYSNPQEFNPSRWDN 420
E+LRRNTI +FRE KT++NIN + IPKGWTV IW A+HMDPQ+YSNP+EF+PSRW+N
Sbjct: 361 ELLRRNTIAVASFREAKTEININDYTIPKGWTVLIWTRAIHMDPQLYSNPEEFDPSRWEN 420
Query: 421 YTPKPGEFIPFGLGSRFCPGSELAKLEITILLHHFILNYKMEGVNLESNNNQMPSPR-PE 480
++PKPG FIPFG+G+RFCPGSEL KLE+TILLHHFILNYKME VN + +P+P+ +
Sbjct: 421 HSPKPGTFIPFGIGTRFCPGSELTKLELTILLHHFILNYKMERVNPKCCITHLPAPKLVD 480
Query: 481 NCLCKIIRL 488
NCLC I +L
Sbjct: 481 NCLCTITKL 485
BLAST of CsGy3G044370 vs. TAIR 10
Match:
AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )
HSP 1 Score: 365.5 bits (937), Expect = 6.5e-101
Identity = 206/482 (42.74%), Postives = 297/482 (61.62%), Query Frame = 0
Query: 12 VVLGVCLL---VRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGYSFSSYKTFIVQEDPIS 71
++LG+ +L ++R+N Y+ KLG + + LPPGDLGWPVIG +S + F DP S
Sbjct: 14 IILGLFVLKWVLKRVNVWIYVSKLGEKKH-YLPPGDLGWPVIGNMWSFLRAF-KTSDPES 73
Query: 72 FIQSLHSRYGKGGMYKTHLYGNPTVIVTNPEICRRIYLDEANFKQHYPKSVKILEG--SS 131
FIQS +RYG+ G+YK H++G P V+VT PE CRR+ D+ F +PKS L G S
Sbjct: 74 FIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSF 133
Query: 132 GDFSNMDHKIAYKVMASPMNGYEVLSNYVDFIEEVIAKGLEEWSSMMREPIKLVDEIGIL 191
S +HK ++ ++P+NG E LS Y+ FIEE + LE+WS M I+ + + L
Sbjct: 134 VGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKM--GEIEFLSHLRKL 193
Query: 192 FFKVITKIFLGSQLDAKTMVELHTLYKELSFGMVMSTFPYDFPGFTFHQLLKARRKIENT 251
FKVI IFL S+ + M L Y L++G + + PGF +H+ LKAR+K+
Sbjct: 194 TFKVIMYIFLSSESE-HVMDSLEREYTNLNYG--VRAMGINLPGFAYHRALKARKKLVAA 253
Query: 252 IQGVVEEKIRRFENDKTSEVQCQVDKLVVAINENGAKLYSNNFIRDLILGIFFAGHSTPA 311
Q +V + + + + +S + +D L+ +ENG L I DL+L AGH +
Sbjct: 254 FQSIVTNRRNQRKQNISSNRKDMLDNLIDVKDENGRVLDDEEII-DLLLMYLNAGHESSG 313
Query: 312 IAACWALLHISQNPHVFQKAKEEQESIIRQRPSVQKGLTLNEIKQMKYLTKIINEVLRRN 371
WA + + ++P + QKAKEEQE I+++R QK LTL E ++M YL+++I+E LR
Sbjct: 314 HLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVI 373
Query: 372 TITATNFRETKTDVNINGHFIPKGWTVQIWNIAVHMDPQIYSNPQEFNPSRWDNYTPKPG 431
T + T FRE K+DV ++G+ IPKGW V W VH+DP+IY +P++F+PSRW+ YTPK G
Sbjct: 374 TFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAG 433
Query: 432 EFIPFGLGSRFCPGSELAKLEITILLHHFILNYKMEGVNLESNNNQMPSPRP-ENCLCKI 488
F+PFGLGS CPG++LAKLEI+I LHHF+L Y++E N +P RP +NCL +I
Sbjct: 434 TFLPFGLGSHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARI 486
BLAST of CsGy3G044370 vs. TAIR 10
Match:
AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )
HSP 1 Score: 365.5 bits (937), Expect = 6.5e-101
Identity = 206/482 (42.74%), Postives = 297/482 (61.62%), Query Frame = 0
Query: 12 VVLGVCLL---VRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGYSFSSYKTFIVQEDPIS 71
++LG+ +L ++R+N Y+ KLG + + LPPGDLGWPVIG +S + F DP S
Sbjct: 14 IILGLFVLKWVLKRVNVWIYVSKLGEKKH-YLPPGDLGWPVIGNMWSFLRAF-KTSDPES 73
Query: 72 FIQSLHSRYGKGGMYKTHLYGNPTVIVTNPEICRRIYLDEANFKQHYPKSVKILEG--SS 131
FIQS +RYG+ G+YK H++G P V+VT PE CRR+ D+ F +PKS L G S
Sbjct: 74 FIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKSF 133
Query: 132 GDFSNMDHKIAYKVMASPMNGYEVLSNYVDFIEEVIAKGLEEWSSMMREPIKLVDEIGIL 191
S +HK ++ ++P+NG E LS Y+ FIEE + LE+WS M I+ + + L
Sbjct: 134 VGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKM--GEIEFLSHLRKL 193
Query: 192 FFKVITKIFLGSQLDAKTMVELHTLYKELSFGMVMSTFPYDFPGFTFHQLLKARRKIENT 251
FKVI IFL S+ + M L Y L++G + + PGF +H+ LKAR+K+
Sbjct: 194 TFKVIMYIFLSSESE-HVMDSLEREYTNLNYG--VRAMGINLPGFAYHRALKARKKLVAA 253
Query: 252 IQGVVEEKIRRFENDKTSEVQCQVDKLVVAINENGAKLYSNNFIRDLILGIFFAGHSTPA 311
Q +V + + + + +S + +D L+ +ENG L I DL+L AGH +
Sbjct: 254 FQSIVTNRRNQRKQNISSNRKDMLDNLIDVKDENGRVLDDEEII-DLLLMYLNAGHESSG 313
Query: 312 IAACWALLHISQNPHVFQKAKEEQESIIRQRPSVQKGLTLNEIKQMKYLTKIINEVLRRN 371
WA + + ++P + QKAKEEQE I+++R QK LTL E ++M YL+++I+E LR
Sbjct: 314 HLTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVI 373
Query: 372 TITATNFRETKTDVNINGHFIPKGWTVQIWNIAVHMDPQIYSNPQEFNPSRWDNYTPKPG 431
T + T FRE K+DV ++G+ IPKGW V W VH+DP+IY +P++F+PSRW+ YTPK G
Sbjct: 374 TFSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAG 433
Query: 432 EFIPFGLGSRFCPGSELAKLEITILLHHFILNYKMEGVNLESNNNQMPSPRP-ENCLCKI 488
F+PFGLGS CPG++LAKLEI+I LHHF+L Y++E N +P RP +NCL +I
Sbjct: 434 TFLPFGLGSHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARI 486
BLAST of CsGy3G044370 vs. TAIR 10
Match:
AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )
HSP 1 Score: 349.0 bits (894), Expect = 6.3e-96
Identity = 189/475 (39.79%), Postives = 286/475 (60.21%), Query Frame = 0
Query: 15 GVCLLVRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGYSFSSYKTFIVQEDPISFIQSLH 74
G+ LVR++N Y LG + LPPGDLGWP IG S + F DP SF ++L
Sbjct: 21 GLNWLVRKVNVWLYESSLGENRH-YLPPGDLGWPFIGNMLSFLRAF-KTSDPDSFTRTLI 80
Query: 75 SRYGKGGMYKTHLYGNPTVIVTNPEICRRIYLDEANFKQHYPKSVKILEG--SSGDFSNM 134
RYG G+YK H++GNP++IVT + CRR+ D+ FK +P S L G S S
Sbjct: 81 KRYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFE 140
Query: 135 DHKIAYKVMASPMNGYEVLSNYVDFIEEVIAKGLEEWSSMMREPIKLVDEIGILFFKVIT 194
+HK ++ A+P+NG+E LS Y+ +IEE + L++W+ M + + + L F++I
Sbjct: 141 EHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKM--GEFEFLTHLRKLTFRIIM 200
Query: 195 KIFLGSQLDAKTMVELHTLYKELSFGMVMSTFPYDFPGFTFHQLLKARRKIENTIQGVVE 254
IFL S+ + M L Y L++G + + PGF +H+ LKAR+ + Q +V
Sbjct: 201 YIFLSSESE-NVMDALEREYTALNYG--VRAMAVNIPGFAYHRALKARKTLVAAFQSIVT 260
Query: 255 EKIRRFENDKTSEVQCQVDKLVVAINENGAKLYSNNFIRDLILGIFFAGHSTPAIAACWA 314
E+ + + + S + +D L+ +E+G L I D++L AGH + WA
Sbjct: 261 ERRNQRKQNILSNKKDMLDNLLNVKDEDGKTLDDEEII-DVLLMYLNAGHESSGHTIMWA 320
Query: 315 LLHISQNPHVFQKAKEEQESIIRQRPSVQKGLTLNEIKQMKYLTKIINEVLRRNTITATN 374
+ + ++P V Q+AK EQE I++ RP QKGL+L E ++M++L+++++E LR T + T
Sbjct: 321 TVFLQEHPEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVITFSLTA 380
Query: 375 FRETKTDVNINGHFIPKGWTVQIWNIAVHMDPQIYSNPQEFNPSRWDN-YTPKPGEFIPF 434
FRE KTDV +NG+ IPKGW V W VH+DP+++ +P++F+P+RWDN + PK G F+PF
Sbjct: 381 FREAKTDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKAGAFLPF 440
Query: 435 GLGSRFCPGSELAKLEITILLHHFILNYKMEGVNLESNNNQMPSPRP-ENCLCKI 486
G GS CPG++LAKLEI+I LHHF+L Y+++ N E +P RP +NCL +I
Sbjct: 441 GAGSHLCPGNDLAKLEISIFLHHFLLKYQVKRSNPECPVMYLPHTRPTDNCLARI 487
BLAST of CsGy3G044370 vs. TAIR 10
Match:
AT3G19270.1 (cytochrome P450, family 707, subfamily A, polypeptide 4 )
HSP 1 Score: 210.3 bits (534), Expect = 3.5e-54
Identity = 146/487 (29.98%), Postives = 240/487 (49.28%), Query Frame = 0
Query: 3 MISVCVIIGVVLGVCLLVRRLNELWYLVKLGGRAYKSLPPGDLGWPVIGYSFSSYKTFIV 62
M + ++ +L +CLL+ R+ + K + LPPG +GWP +G + Y
Sbjct: 1 MAEIWFLVVPILILCLLLVRV----IVSKKKKNSRGKLPPGSMGWPYLGETLQLY----- 60
Query: 63 QEDPISFIQSLHSRYGKGGMYKTHLYGNPTVIVTNPEICRRIYLDEAN-FKQHYPKSVKI 122
++P F S RYG+ ++KT + G P V++ +PE R + + A+ FK YP+S +
Sbjct: 61 SQNPNVFFTSKQKRYGE--IFKTRILGYPCVMLASPEAARFVLVTHAHMFKPTYPRSKEK 120
Query: 123 LEGSSGDFSNM-DHKIAYKVMASPMNGYEVLSNYVDFIEEVIAKGLEEWSSM-----MRE 182
L G S F + D+ + + E + + IE + L+ W++M +E
Sbjct: 121 LIGPSALFFHQGDYHSHIRKLVQSSFYPETIRKLIPDIEHIALSSLQSWANMPIVSTYQE 180
Query: 183 PIKLVDEIGILFFKVITKIFLGSQLDAKTMVELHTLYKELSFGMVMSTFPYDFPGFTFHQ 242
K ++GIL IF L++ L Y + G ++FP PG ++H+
Sbjct: 181 MKKFAFDVGIL------AIF--GHLESSYKEILKHNYNIVDKG--YNSFPMSLPGTSYHK 240
Query: 243 LLKARRKIENTIQGVVEEKIRRFENDKTSEVQCQVDKLVVAINENGAKLYSNNFIRDLIL 302
L AR++++ + ++ E+ +K + + L+ NE G ++ + I D I+
Sbjct: 241 ALMARKQLKTIVSEIICER-----REKRALQTDFLGHLLNFKNEKG-RVLTQEQIADNII 300
Query: 303 GIFFAGHSTPAIAACWALLHISQNPHVFQKAKEEQESIIRQRPSVQKGLTLNEIKQMKYL 362
G+ FA T A W L ++ + + + K EQ++I + +K LT + + M
Sbjct: 301 GVLFAAQDTTASCLTWILKYLHDDQKLLEAVKAEQKAIYEENSREKKPLTWRQTRNMPLT 360
Query: 363 TKIINEVLRRNTITATNFRETKTDVNINGHFIPKGWTVQIWNIAVHMDPQIYSNPQEFNP 422
K+I E LR +I + FRE DV G+ IPKGW V +H +P+ +SNP+ F+P
Sbjct: 361 HKVIVESLRMASIISFTFREAVVDVEYKGYLIPKGWKVMPLFRNIHHNPKYFSNPEVFDP 420
Query: 423 SRWDNYTPKPGEFIPFGLGSRFCPGSELAKLEITILLHHFILNYKMEGVNLESNNNQMPS 482
SR++ PKP F+PFG G CPG+ELAKL+I I LHH + N++ E E P
Sbjct: 421 SRFE-VNPKPNTFMPFGSGVHACPGNELAKLQILIFLHHLVSNFRWEVKGGEKGIQYSPF 459
BLAST of CsGy3G044370 vs. TAIR 10
Match:
AT4G19230.1 (cytochrome P450, family 707, subfamily A, polypeptide 1 )
HSP 1 Score: 206.8 bits (525), Expect = 3.9e-53
Identity = 137/453 (30.24%), Postives = 217/453 (47.90%), Query Frame = 0
Query: 34 GRAYKSLPPGDLGWPVIGYSFSSYKTFIVQEDPISFIQSLHSRYGKGGMYKTHLYGNPTV 93
G + LPPG +GWP +G +F Y +DP F QS RY G ++KTH+ G P V
Sbjct: 30 GSSKLPLPPGTMGWPYVGETFQLY-----SQDPNVFFQSKQKRY--GSVFKTHVLGCPCV 89
Query: 94 IVTNPEICRRIYLDEAN-FKQHYPKSVKILEGSSGDFSNM-DHKIAYKVMASPMNGYEVL 153
++++PE + + + +++ FK +P S + + G F + D+ + + E +
Sbjct: 90 MISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHAKLRKLVLRAFMPESI 149
Query: 154 SNYVDFIEEVIAKGLEEWSSMMREPIKLVDEIGILFFKVITKIFLGSQLDAKTMVELHTL 213
N V IE + L W M I E+ F V G + +L
Sbjct: 150 RNMVPDIESIAQDSLRSWEGTM---INTYQEMKTYTFNVALLSIFGKD-EVLYREDLKRC 209
Query: 214 YKELSFGMVMSTFPYDFPGFTFHQLLKARRKIENTIQGVVEEKIRR--FENDKTSEVQCQ 273
Y L G ++ P + PG FH+ +KAR+++ + ++ E+ + ND
Sbjct: 210 YYILEKG--YNSMPVNLPGTLFHKSMKARKELSQILARILSERRQNGSSHNDLLGSFMGD 269
Query: 274 VDKLVVAINENGAKLYSNNFIRDLILGIFFAGHSTPAIAACWALLHISQNPHVFQKAKEE 333
++L ++ I D I+G+ FA T A W L ++++NP+V + EE
Sbjct: 270 KEEL------------TDEQIADNIIGVIFAARDTTASVMSWILKYLAENPNVLEAVTEE 329
Query: 334 QESIIRQRPSVQKGLTLNEIKQMKYLTKIINEVLRRNTITATNFRETKTDVNINGHFIPK 393
Q + IR+ + LT + K+M +++I E LR +I + FRE DV G+ IPK
Sbjct: 330 QMA-IRKDKEEGESLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDVEYEGYLIPK 389
Query: 394 GWTVQIWNIAVHMDPQIYSNPQEFNPSRWDNYTPKPGEFIPFGLGSRFCPGSELAKLEIT 453
GW V +H I+SNP +F+PSR++ PKP F+PFG G+ CPG+ELAKLE++
Sbjct: 390 GWKVLPLFRNIHHSADIFSNPGKFDPSRFE-VAPKPNTFMPFGNGTHSCPGNELAKLEMS 449
Query: 454 ILLHHFILNYKMEGVNLESNNNQMPSPRPENCL 483
I++HH Y V P P+N L
Sbjct: 450 IMIHHLTTKYSWSIVGASDGIQYGPFALPQNGL 455
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
B5BSX1 | 4.5e-115 | 44.69 | Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1 | [more] |
Q9C5Y2 | 9.1e-100 | 42.74 | Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2 | [more] |
Q5VRM7 | 2.0e-99 | 37.20 | Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 ... | [more] |
Q9AXH9 | 4.2e-97 | 37.17 | Ent-kaurenoic acid oxidase 1 OS=Hordeum vulgare OX=4513 GN=KAO1 PE=1 SV=1 | [more] |
O23051 | 8.9e-95 | 39.79 | Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A097IYP6 | 0.0 | 100.00 | Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa3G903550 PE=2 SV=1 | [more] |
A0A1S3CRX8 | 1.85e-303 | 89.32 | beta-amyrin 11-oxidase-like OS=Cucumis melo OX=3656 GN=LOC103504002 PE=3 SV=1 | [more] |
A0A6J1GJM1 | 8.02e-235 | 65.64 | beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454910 PE=3 S... | [more] |
A0A6J1GLR1 | 6.32e-234 | 66.46 | beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111455409 PE=3 S... | [more] |
A0A6J1GJP8 | 2.43e-231 | 65.03 | beta-amyrin 11-oxidase-like OS=Cucurbita moschata OX=3662 GN=LOC111454912 PE=3 S... | [more] |