CsGy3G042020 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy3G042020
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Descriptionrust resistance kinase Lr10-like
LocationGy14Chr3: 39212017 .. 39216067 (+)
RNA-Seq ExpressionCsGy3G042020
SyntenyCsGy3G042020
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CATGAAAATAATATTAAAAAAGACTTAAAAAATTAGAAGTTAATACCAATAACAACAAGGTGCACAAGAATGGTTTTAGTTGTTAAACTCTCTATATTTGTAGGTATAAACTGATGGTTTTTCCTCTCAATTTTATATATTAAAGTTCAATGAATTTAAAATAGAAGTTGACTTGACTTCCATACAAAACTCATAACAATTTTAAAATTTGAGGCTTCTTCTCTCTCTCTTGTTCCTTCCCATTCACCCAATCTTCTTTCTCTCAATCAACAATGAAAAATATCAAATTCCAGATTTCCCAATTGAAGAAGTCAACCATGAAAATTTCAACGTTACTTCCGATTCTTCCTCACTCTTCATGAATCTGATCATGTCATGCATTTCCTTTTTCAGTACATACCCATTTTTGCTCACAAAACTCTTGCCACTTTCAAGCTCATTCAGAGGCGTTAAGAAATGATCGAAATCGCTCTGTTATACCAGTAAGTATCTTCCACTCTATGTTGGATGGTATTCCTATATCTGAGTTATCGATTTCAGTTCTTTTTCTTTCTTTTTTGTAATTTCAGATCCTCATCCTCCCAAGACAACGATCAAGTAAAGTCGATTACTGTTTTGTGTAAGCATACAATATCTCTAATCTCGTTGAAGCTGAGAGGTTTCTTATTCTGTTCTGAAAATCAAAGAAGCTCTTTGGTTCTATATTTTTTTTTATTACTTGTTAGAAAAGAATACTTGCATCAGTTTGTAAATGGGTATGAAGTGTGAGACTGTAACCATTCCAATTTCTCCTAACTTTTGAACACTTTTGATTGAATTAGCTGTTGTTGACTTTCAGGGGTGGTGGGCTCAGTGGTTTCCTTGGTGGTTTTTGTGGTGATTGTCATATGTGCTCTTAAGTTTGCCAAAAGCGCTGCCCCAGGTATTGTAAGAGAACTGAGGAACATAACTGCATCTCCTGTGGTTATAGCTGCTCCTCCTGTAACTGTGCCTTATAGAACCGCACCTCCTAGAGCTGCTTCTCCTTCACTTGAACTTAGCAGGGCTTCTGCAGCTATTTGGCGAGTCGATGCACCAACAATGGAGAAATTCTTCCGCAAGATTGCCGAAGAAAAACCAGTCAGATTCACTGCTGACCAACTCTATACCTTCACAAGCAACTACTCGGCTCGGCTTGGATCTGGAGGTTTTGGTGAGGTTTACAAAGGGAAGTTCCCAAATGGGGTGAATATTGCTGTGAAGGTTTTGAAGAGAGGCTCAGATAAAAGAGCTGAAGAGCAATTCATGGCTGAGGTAGGATCAATTTGTAGGACTTATCACATTAATTTAGTAAGGCTGTATGGTTTTTGCTATGACCATTTCATGAGTGCACTTGTTTTCGAATACTTAGTAAATGGATCTTTGGACAAGTACTTGTTTGGAAAAAGAAAAGAAATTGAATGGAGAAAATTGCATGATATAGCAATTGGAACAGCCAAAGGTCTTGCTTATCTTCATGAGGAATGTCAACAGAGAATCATTCACTATGATATTAAGCCAGCAAACATTCTTCTAGATGCAAACTTCTCCCCCAAAGTTGGTGATTTTGGGCTTGCCAAGCTCTGCAATAGGGACAGCACACACATCTCGTTCAGCGGCTATCGAGGCACTCCCGGTTACTCAGCGCCTGAATTTCTGCTGGTTAACTATCCATTAACACACAAGTGCGATGTGTATAGCTTTGGAATGGTGTTGTTTGAGATCATTGGAAGAAAGAGCAATGCTGGCACAACTGTTTCCGGGAATCCTGATTGGTTCCCACAGCATGTGTGGGATGCTTATGAAAACGGTAAGTTGGAAGAGTTGATTATTGGTTGTGGCATTGGGGAGGAAGATAGGGAGATGGCAAGCCGAGCATGTGAGGTGGCTCTTTGGTGCGTTCAGGACTCCCCTGACGGGCGTCCAACGATGAGTGTAGTGGTGAGGATGTTGGAGGGAGGAGTTGAGATAATGCCACCGGCAAAACCATTCCAATATCTGAACCCCATAAGAACGGGGAATAGTTCAACAAGTTCACAACAAACAGGCAGTAATAGTTCAGGCTCTTCAACAAGCAGAGACAGTAATTCTCAATGGTACAAGAAGACTACAACAATCATGAGAAAATACGAAATACAAATGGCTAGCTCTTGATCTTTGCTAAACAAACTTGTATAATCTTGTGGAAAAGCCTTCATCTGTCTACAAATTCGTTATTGTTACTCGTATTTCGATTTGAAATCATAATTTAAATGTTGTAAACTCGTTAAGTTTGTAAACACTCAAGAACACAATCCAAACATATCTTTCTTTAAGTTTTTTTTGGCTAGACTATAGTGACAATAGATTGTATTCATT

mRNA sequence

CATGAAAATAATATTAAAAAAGACTTAAAAAATTAGAAGTTAATACCAATAACAACAAGGTGCACAAGAATGGTTTTAGTTGTTAAACTCTCTATATTTGTAGGTATAAACTGATGGTTTTTCCTCTCAATTTTATATATTAAAGTTCAATGAATTTAAAATAGAAGTTGACTTGACTTCCATACAAAACTCATAACAATTTTAAAATTTGAGGCTTCTTCTCTCTCTCTTGTTCCTTCCCATTCACCCAATCTTCTTTCTCTCAATCAACAATGAAAAATATCAAATTCCAGATTTCCCAATTGAAGAAGTCAACCATGAAAATTTCAACGTTACTTCCGATTCTTCCTCACTCTTCATGAATCTGATCATGTCATGCATTTCCTTTTTCAGTACATACCCATTTTTGCTCACAAAACTCTTGCCACTTTCAAGCTCATTCAGAGGCGTTAAGAAATGATCGAAATCGCTCTGTTATACCAGTAAGTATCTTCCACTCTATGTTGGATGGTATTCCTATATCTGAGTTATCGATTTCAGTTCTTTTTCTTTCTTTTTTGTAATTTCAGATCCTCATCCTCCCAAGACAACGATCAAGTAAAGTCGATTACTGTTTTGTGGGTGGTGGGCTCAGTGGTTTCCTTGGTGGTTTTTGTGGTGATTGTCATATGTGCTCTTAAGTTTGCCAAAAGCGCTGCCCCAGGTATTGTAAGAGAACTGAGGAACATAACTGCATCTCCTGTGGTTATAGCTGCTCCTCCTGTAACTGTGCCTTATAGAACCGCACCTCCTAGAGCTGCTTCTCCTTCACTTGAACTTAGCAGGGCTTCTGCAGCTATTTGGCGAGTCGATGCACCAACAATGGAGAAATTCTTCCGCAAGATTGCCGAAGAAAAACCAGTCAGATTCACTGCTGACCAACTCTATACCTTCACAAGCAACTACTCGGCTCGGCTTGGATCTGGAGGTTTTGGTGAGGTTTACAAAGGGAAGTTCCCAAATGGGGTGAATATTGCTGTGAAGGTTTTGAAGAGAGGCTCAGATAAAAGAGCTGAAGAGCAATTCATGGCTGAGGTAGGATCAATTTGTAGGACTTATCACATTAATTTAGTAAGGCTGTATGGTTTTTGCTATGACCATTTCATGAGTGCACTTGTTTTCGAATACTTAGTAAATGGATCTTTGGACAAGTACTTGTTTGGAAAAAGAAAAGAAATTGAATGGAGAAAATTGCATGATATAGCAATTGGAACAGCCAAAGGTCTTGCTTATCTTCATGAGGAATGTCAACAGAGAATCATTCACTATGATATTAAGCCAGCAAACATTCTTCTAGATGCAAACTTCTCCCCCAAAGTTGGTGATTTTGGGCTTGCCAAGCTCTGCAATAGGGACAGCACACACATCTCGTTCAGCGGCTATCGAGGCACTCCCGGTTACTCAGCGCCTGAATTTCTGCTGGTTAACTATCCATTAACACACAAGTGCGATGTGTATAGCTTTGGAATGGTGTTGTTTGAGATCATTGGAAGAAAGAGCAATGCTGGCACAACTGTTTCCGGGAATCCTGATTGGTTCCCACAGCATGTGTGGGATGCTTATGAAAACGGTAAGTTGGAAGAGTTGATTATTGGTTGTGGCATTGGGGAGGAAGATAGGGAGATGGCAAGCCGAGCATGTGAGGTGGCTCTTTGGTGCGTTCAGGACTCCCCTGACGGGCGTCCAACGATGAGTGTAGTGGTGAGGATGTTGGAGGGAGGAGTTGAGATAATGCCACCGGCAAAACCATTCCAATATCTGAACCCCATAAGAACGGGGAATAGTTCAACAAGTTCACAACAAACAGGCAGTAATAGTTCAGGCTCTTCAACAAGCAGAGACAGTAATTCTCAATGGTACAAGAAGACTACAACAATCATGAGAAAATACGAAATACAAATGGCTAGCTCTTGATCTTTGCTAAACAAACTTGTATAATCTTGTGGAAAAGCCTTCATCTGTCTACAAATTCGTTATTGTTACTCGTATTTCGATTTGAAATCATAATTTAAATGTTGTAAACTCGTTAAGTTTGTAAACACTCAAGAACACAATCCAAACATATCTTTCTTTAAGTTTTTTTTGGCTAGACTATAGTGACAATAGATTGTATTCATT

Coding sequence (CDS)

ATGGTATTCCTATATCTGAGTTATCGATTTCAGTTCTTTTTCTTTCTTTTTTGTAATTTCAGATCCTCATCCTCCCAAGACAACGATCAAGTAAAGTCGATTACTGTTTTGTGGGTGGTGGGCTCAGTGGTTTCCTTGGTGGTTTTTGTGGTGATTGTCATATGTGCTCTTAAGTTTGCCAAAAGCGCTGCCCCAGGTATTGTAAGAGAACTGAGGAACATAACTGCATCTCCTGTGGTTATAGCTGCTCCTCCTGTAACTGTGCCTTATAGAACCGCACCTCCTAGAGCTGCTTCTCCTTCACTTGAACTTAGCAGGGCTTCTGCAGCTATTTGGCGAGTCGATGCACCAACAATGGAGAAATTCTTCCGCAAGATTGCCGAAGAAAAACCAGTCAGATTCACTGCTGACCAACTCTATACCTTCACAAGCAACTACTCGGCTCGGCTTGGATCTGGAGGTTTTGGTGAGGTTTACAAAGGGAAGTTCCCAAATGGGGTGAATATTGCTGTGAAGGTTTTGAAGAGAGGCTCAGATAAAAGAGCTGAAGAGCAATTCATGGCTGAGGTAGGATCAATTTGTAGGACTTATCACATTAATTTAGTAAGGCTGTATGGTTTTTGCTATGACCATTTCATGAGTGCACTTGTTTTCGAATACTTAGTAAATGGATCTTTGGACAAGTACTTGTTTGGAAAAAGAAAAGAAATTGAATGGAGAAAATTGCATGATATAGCAATTGGAACAGCCAAAGGTCTTGCTTATCTTCATGAGGAATGTCAACAGAGAATCATTCACTATGATATTAAGCCAGCAAACATTCTTCTAGATGCAAACTTCTCCCCCAAAGTTGGTGATTTTGGGCTTGCCAAGCTCTGCAATAGGGACAGCACACACATCTCGTTCAGCGGCTATCGAGGCACTCCCGGTTACTCAGCGCCTGAATTTCTGCTGGTTAACTATCCATTAACACACAAGTGCGATGTGTATAGCTTTGGAATGGTGTTGTTTGAGATCATTGGAAGAAAGAGCAATGCTGGCACAACTGTTTCCGGGAATCCTGATTGGTTCCCACAGCATGTGTGGGATGCTTATGAAAACGGTAAGTTGGAAGAGTTGATTATTGGTTGTGGCATTGGGGAGGAAGATAGGGAGATGGCAAGCCGAGCATGTGAGGTGGCTCTTTGGTGCGTTCAGGACTCCCCTGACGGGCGTCCAACGATGAGTGTAGTGGTGAGGATGTTGGAGGGAGGAGTTGAGATAATGCCACCGGCAAAACCATTCCAATATCTGAACCCCATAAGAACGGGGAATAGTTCAACAAGTTCACAACAAACAGGCAGTAATAGTTCAGGCTCTTCAACAAGCAGAGACAGTAATTCTCAATGGTACAAGAAGACTACAACAATCATGAGAAAATACGAAATACAAATGGCTAGCTCTTGA

Protein sequence

MVFLYLSYRFQFFFFLFCNFRSSSSQDNDQVKSITVLWVVGSVVSLVVFVVIVICALKFAKSAAPGIVRELRNITASPVVIAAPPVTVPYRTAPPRAASPSLELSRASAAIWRVDAPTMEKFFRKIAEEKPVRFTADQLYTFTSNYSARLGSGGFGEVYKGKFPNGVNIAVKVLKRGSDKRAEEQFMAEVGSICRTYHINLVRLYGFCYDHFMSALVFEYLVNGSLDKYLFGKRKEIEWRKLHDIAIGTAKGLAYLHEECQQRIIHYDIKPANILLDANFSPKVGDFGLAKLCNRDSTHISFSGYRGTPGYSAPEFLLVNYPLTHKCDVYSFGMVLFEIIGRKSNAGTTVSGNPDWFPQHVWDAYENGKLEELIIGCGIGEEDREMASRACEVALWCVQDSPDGRPTMSVVVRMLEGGVEIMPPAKPFQYLNPIRTGNSSTSSQQTGSNSSGSSTSRDSNSQWYKKTTTIMRKYEIQMASS*
Homology
BLAST of CsGy3G042020 vs. ExPASy Swiss-Prot
Match: P93604 (Rust resistance kinase Lr10 OS=Triticum aestivum OX=4565 GN=LRK10 PE=2 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 1.8e-68
Identity = 131/318 (41.19%), Postives = 197/318 (61.95%), Query Frame = 0

Query: 119 MEKFFRKIAEEKPVRFTADQLYTFTSNYSARLGSGGFGEVYKGKFPNGVNIAVKVLKRGS 178
           +E F +     KP R+T  ++      +  ++G GGFG VYKG+ PNGV +AVK+L+  +
Sbjct: 314 VEMFLKTYGTSKPTRYTFSEVKKMARRFKEKVGQGGFGSVYKGELPNGVPVAVKMLENST 373

Query: 179 DKRAEEQFMAEVGSICRTYHINLVRLYGFCYDHFMSALVFEYLVNGSLDKYLFGKRKEIE 238
            +   E F+ EV +I   +H N+VRL GFC +    AL++E++ N SL+KY+F     I 
Sbjct: 374 GE--GESFINEVATIGLIHHANIVRLLGFCSEGMRRALIYEFMPNESLEKYIFSDDSNIF 433

Query: 239 W-----RKLHDIAIGTAKGLAYLHEECQQRIIHYDIKPANILLDANFSPKVGDFGLAKLC 298
                  KL DIA+G A+G+ YLH+ C QRI+H+DIKP NILLD NF+PK+ DFGLAKLC
Sbjct: 434 QNLLVPEKLLDIALGIARGMEYLHQGCNQRILHFDIKPHNILLDYNFNPKISDFGLAKLC 493

Query: 299 NRDSTHISFSGYRGTPGYSAPEFLLVNY-PLTHKCDVYSFGMVLFEIIGRKSNAGTTVSG 358
            RD + ++ +  RGT GY APE    N+  +++K DVYSFGM++ E++  + N+   +  
Sbjct: 494 ARDQSIVTLTAARGTMGYIAPELYSRNFGGVSYKADVYSFGMLVLEMVSGRRNSDPRIGS 553

Query: 359 NPD-WFPQHVWDAYENGKLEELIIGCGIGEEDREMASRACEVALWCVQDSPDGRPTMSVV 418
             D + P+ +++   NG  EEL +     +E+++   +   VALWC+Q +P  RP+M+ V
Sbjct: 554 QDDVYLPEWIYEKVING--EELALTLETTQEEKDKVRQLAMVALWCIQWNPRNRPSMTKV 613

Query: 419 VRMLEGGVE-IMPPAKPF 429
           V ML G ++ +  P KPF
Sbjct: 614 VNMLTGRLQSLQMPPKPF 627

BLAST of CsGy3G042020 vs. ExPASy Swiss-Prot
Match: Q9FF29 (PR5-like receptor kinase OS=Arabidopsis thaliana OX=3702 GN=PR5K PE=1 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 6.9e-68
Identity = 131/300 (43.67%), Postives = 193/300 (64.33%), Query Frame = 0

Query: 133 RFTADQLYTFTSNYSARLGSGGFGEVYKGKF-PNGVNIAVKVLKRGSDKRAEEQFMAEVG 192
           R++  ++   T++++  LG GGFG VYKGK   +G ++AVK+LK        E+F+ EV 
Sbjct: 320 RYSYTRVKKMTNSFAHVLGKGGFGTVYKGKLADSGRDVAVKILKVSEGN--GEEFINEVA 379

Query: 193 SICRTYHINLVRLYGFCYDHFMSALVFEYLVNGSLDKYLFGKRK-EIEWRKLHDIAIGTA 252
           S+ RT H+N+V L GFCY+    A+++E++ NGSLDKY+      ++EW +L+D+A+G +
Sbjct: 380 SMSRTSHVNIVSLLGFCYEKNKRAIIYEFMPNGSLDKYISANMSTKMEWERLYDVAVGIS 439

Query: 253 KGLAYLHEECQQRIIHYDIKPANILLDANFSPKVGDFGLAKLCNRDSTHISFSGYRGTPG 312
           +GL YLH  C  RI+H+DIKP NIL+D N  PK+ DFGLAKLC    + IS    RGT G
Sbjct: 440 RGLEYLHNRCVTRIVHFDIKPQNILMDENLCPKISDFGLAKLCKNKESIISMLHMRGTFG 499

Query: 313 YSAPEFLLVNY-PLTHKCDVYSFGMVLFEIIGRKSNAGTTVSGNPD---WFPQHVWDAYE 372
           Y APE    N+  ++HK DVYS+GMV+ E+IG K+      SG+ +   +FP+ V+  +E
Sbjct: 500 YIAPEMFSKNFGAVSHKSDVYSYGMVVLEMIGAKNIEKVEYSGSNNGSMYFPEWVYKDFE 559

Query: 373 NGKLEELIIGCGIGEEDREMASRACEVALWCVQDSPDGRPTMSVVVRMLEGGVEIM--PP 425
            G++   I G  I +E+ ++A +   VALWC+Q +P  RP M  V+ MLEG +E +  PP
Sbjct: 560 KGEITR-IFGDSITDEEEKIAKKLVLVALWCIQMNPSDRPPMIKVIEMLEGNLEALQVPP 616

BLAST of CsGy3G042020 vs. ExPASy Swiss-Prot
Match: Q9FLV4 (G-type lectin S-receptor-like serine/threonine-protein kinase At5g24080 OS=Arabidopsis thaliana OX=3702 GN=At5g24080 PE=2 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 5.0e-66
Identity = 136/313 (43.45%), Postives = 186/313 (59.42%), Query Frame = 0

Query: 129 EKPVRFTADQLYTFTSNYSARLGSGGFGEVYKGKFPNGVNIAVKVLKRGSDKRAEEQFMA 188
           + PV FT   L   T+N+S  LGSGGFG VYKG       +AVK L R      E +F+ 
Sbjct: 515 DSPVSFTYRDLQNCTNNFSQLLGSGGFGTVYKGTVAGETLVAVKRLDRAL-SHGEREFIT 574

Query: 189 EVGSICRTYHINLVRLYGFCYDHFMSALVFEYLVNGSLDKYLFGKRKE---IEWRKLHDI 248
           EV +I   +H+NLVRL G+C +     LV+EY++NGSLDK++F   +    ++WR   +I
Sbjct: 575 EVNTIGSMHHMNLVRLCGYCSEDSHRLLVYEYMINGSLDKWIFSSEQTANLLDWRTRFEI 634

Query: 249 AIGTAKGLAYLHEECQQRIIHYDIKPANILLDANFSPKVGDFGLAKLCNRDSTHISFSGY 308
           A+ TA+G+AY HE+C+ RIIH DIKP NILLD NF PKV DFGLAK+  R+ +H+  +  
Sbjct: 635 AVATAQGIAYFHEQCRNRIIHCDIKPENILLDDNFCPKVSDFGLAKMMGREHSHV-VTMI 694

Query: 309 RGTPGYSAPEFLLVNYPLTHKCDVYSFGMVLFEIIGRKSNAGTTVSGNPDWFPQHVWDAY 368
           RGT GY APE+ + N P+T K DVYS+GM+L EI+G + N   +      ++P   +   
Sbjct: 695 RGTRGYLAPEW-VSNRPITVKADVYSYGMLLLEIVGGRRNLDMSYDAEDFFYPGWAYKEL 754

Query: 369 ENGKLEELIIGCGIGEEDREMASRACEVALWCVQDSPDGRPTMSVVVRMLEG-GVEIMPP 428
            NG   + +     G  + E   +A +VA WC+QD    RP+M  VV++LEG   EI  P
Sbjct: 755 TNGTSLKAVDKRLQGVAEEEEVVKALKVAFWCIQDEVSMRPSMGEVVKLLEGTSDEINLP 814

Query: 429 AKPFQYLNPIRTG 438
             P   L  I  G
Sbjct: 815 PMPQTILELIEEG 824

BLAST of CsGy3G042020 vs. ExPASy Swiss-Prot
Match: Q8RWZ5 (G-type lectin S-receptor-like serine/threonine-protein kinase SD2-5 OS=Arabidopsis thaliana OX=3702 GN=SD25 PE=1 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 6.5e-66
Identity = 144/339 (42.48%), Postives = 204/339 (60.18%), Query Frame = 0

Query: 131 PVRFTADQLYTFTSNYSARLGSGGFGEVYKGKFPNGVNIAVKVLKRGSDKRAEEQFMAEV 190
           P+RF    L + T+N+S +LG GGFG VY+G  P+G  +AVK L+     + +++F AEV
Sbjct: 480 PIRFAYKDLQSATNNFSVKLGQGGFGSVYEGTLPDGSRLAVKKLE--GIGQGKKEFRAEV 539

Query: 191 GSICRTYHINLVRLYGFCYDHFMSALVFEYLVNGSLDKYLFGKRKE---IEWRKLHDIAI 250
             I   +H++LVRL GFC +     L +E+L  GSL++++F K+     ++W    +IA+
Sbjct: 540 SIIGSIHHLHLVRLRGFCAEGAHRLLAYEFLSKGSLERWIFRKKDGDVLLDWDTRFNIAL 599

Query: 251 GTAKGLAYLHEECQQRIIHYDIKPANILLDANFSPKVGDFGLAKLCNRDSTHISFSGYRG 310
           GTAKGLAYLHE+C  RI+H DIKP NILLD NF+ KV DFGLAKL  R+ +H+ F+  RG
Sbjct: 600 GTAKGLAYLHEDCDARIVHCDIKPENILLDDNFNAKVSDFGLAKLMTREQSHV-FTTMRG 659

Query: 311 TPGYSAPEFLLVNYPLTHKCDVYSFGMVLFEIIGRKSNAGTTVSGNPDWFPQHVWDAYEN 370
           T GY APE+ + NY ++ K DVYS+GMVL E+IG + N   + +     FP   +   E 
Sbjct: 660 TRGYLAPEW-ITNYAISEKSDVYSYGMVLLELIGGRKNYDPSETSEKCHFPSFAFKKMEE 719

Query: 371 GKLEELIIG--CGIGEEDREMASRACEVALWCVQDSPDGRPTMSVVVRMLEGGVEIMPPA 430
           GKL +++ G    +   D E   RA + ALWC+Q+    RP+MS VV+MLEG   ++ P 
Sbjct: 720 GKLMDIVDGKMKNVDVTD-ERVQRAMKTALWCIQEDMQTRPSMSKVVQMLEGVFPVVQP- 779

Query: 431 KPFQYLNPIRTGNSSTSSQQTGSNSSGSSTSRDSNSQWY 465
            P       R  +S   S      ++ SS   D NS+ Y
Sbjct: 780 -PSSSTMGSRLYSSFFKSISEDGGATTSSGPSDCNSENY 811

BLAST of CsGy3G042020 vs. ExPASy Swiss-Prot
Match: Q9XID3 (G-type lectin S-receptor-like serine/threonine-protein kinase At1g34300 OS=Arabidopsis thaliana OX=3702 GN=At1g34300 PE=1 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 1.4e-65
Identity = 143/335 (42.69%), Postives = 195/335 (58.21%), Query Frame = 0

Query: 127 AEEKPVRFTADQLYTFTSNYSARLGSGGFGEVYKGKFPNGVNIAVKVLKRGSDKRAEEQF 186
           A   PV+FT  +L   T ++  +LG+GGFG VY+G   N   +AVK L+    ++ E+QF
Sbjct: 467 ASGAPVQFTYKELQRCTKSFKEKLGAGGFGTVYRGVLTNRTVVAVKQLE--GIEQGEKQF 526

Query: 187 MAEVGSICRTYHINLVRLYGFCYDHFMSALVFEYLVNGSLDKYLF--GKRKEIEWRKLHD 246
             EV +I  T+H+NLVRL GFC       LV+E++ NGSLD +LF     K + W    +
Sbjct: 527 RMEVATISSTHHLNLVRLIGFCSQGRHRLLVYEFMRNGSLDNFLFTTDSAKFLTWEYRFN 586

Query: 247 IAIGTAKGLAYLHEECQQRIIHYDIKPANILLDANFSPKVGDFGLAKLCNRDSTHISFSG 306
           IA+GTAKG+ YLHEEC+  I+H DIKP NIL+D NF+ KV DFGLAKL N      + S 
Sbjct: 587 IALGTAKGITYLHEECRDCIVHCDIKPENILVDDNFAAKVSDFGLAKLLNPKDNRYNMSS 646

Query: 307 YRGTPGYSAPEFLLVNYPLTHKCDVYSFGMVLFEIIGRKSNAGTTVSGNPDWFPQHVWDA 366
            RGT GY APE+ L N P+T K DVYS+GMVL E++  K N   +   N   F    ++ 
Sbjct: 647 VRGTRGYLAPEW-LANLPITSKSDVYSYGMVLLELVSGKRNFDVSEKTNHKKFSIWAYEE 706

Query: 367 YENGKLEELIIGCGIGEE---DREMASRACEVALWCVQDSPDGRPTMSVVVRMLEGGVEI 426
           +E G   + I+   + E+   D E   R  + + WC+Q+ P  RPTM  VV+MLEG  EI
Sbjct: 707 FEKGN-TKAILDTRLSEDQTVDMEQVMRMVKTSFWCIQEQPLQRPTMGKVVQMLEGITEI 766

Query: 427 MPPAKPFQYLNPIRTGNSSTSSQQTGSNSSGSSTS 457
             P  P        +GNS ++S  +   +SG + S
Sbjct: 767 KNPLCPKTISEVSFSGNSMSTSHASMFVASGPTRS 797

BLAST of CsGy3G042020 vs. NCBI nr
Match: XP_031738771.1 (rust resistance kinase Lr10 isoform X1 [Cucumis sativus])

HSP 1 Score: 950 bits (2456), Expect = 0.0
Identity = 478/481 (99.38%), Postives = 479/481 (99.58%), Query Frame = 0

Query: 1   MVFLYLSYRFQFFFFLFCNFRSSSSQDNDQVKSITVLWVVGSVVSLVVFVVIVICALKFA 60
           MVFL+LSYRFQFFFFLFCNFRSSSSQDNDQVKSITVLWVVGSVVSLVVFVVIVICALKFA
Sbjct: 1   MVFLHLSYRFQFFFFLFCNFRSSSSQDNDQVKSITVLWVVGSVVSLVVFVVIVICALKFA 60

Query: 61  KSAAPGIVRELRNITASPVVIAAPPVTVPYRTAPPRAASPSLELSRASAAIWRVDAPTME 120
           KSAAPGIVRELRNITASPVVIAAPPVTVPYRTAPPRAASPSLELSRAS AIWRVDAPTME
Sbjct: 61  KSAAPGIVRELRNITASPVVIAAPPVTVPYRTAPPRAASPSLELSRASEAIWRVDAPTME 120

Query: 121 KFFRKIAEEKPVRFTADQLYTFTSNYSARLGSGGFGEVYKGKFPNGVNIAVKVLKRGSDK 180
           KFFRKIAEEKPVRFTADQLYTFTSNYS RLGSGGFGEVYKGKFPNGVNIAVKVLKRGSDK
Sbjct: 121 KFFRKIAEEKPVRFTADQLYTFTSNYSTRLGSGGFGEVYKGKFPNGVNIAVKVLKRGSDK 180

Query: 181 RAEEQFMAEVGSICRTYHINLVRLYGFCYDHFMSALVFEYLVNGSLDKYLFGKRKEIEWR 240
           RAEEQFMAEVGSICRTYHINLVRLYGFCYDHFMSALVFEYLVNGSLDKYLFGKRKEIEWR
Sbjct: 181 RAEEQFMAEVGSICRTYHINLVRLYGFCYDHFMSALVFEYLVNGSLDKYLFGKRKEIEWR 240

Query: 241 KLHDIAIGTAKGLAYLHEECQQRIIHYDIKPANILLDANFSPKVGDFGLAKLCNRDSTHI 300
           KLHDIAIGTAKGLAYLHEECQQRIIHYDIKPANILLDANFSPKVGDFGLAKLCNRDSTHI
Sbjct: 241 KLHDIAIGTAKGLAYLHEECQQRIIHYDIKPANILLDANFSPKVGDFGLAKLCNRDSTHI 300

Query: 301 SFSGYRGTPGYSAPEFLLVNYPLTHKCDVYSFGMVLFEIIGRKSNAGTTVSGNPDWFPQH 360
           SFSGYRGTPGYSAPEFLLVNYPLTHKCDVYSFGMVLFEIIGRKSNAGTTVSGNPDWFPQH
Sbjct: 301 SFSGYRGTPGYSAPEFLLVNYPLTHKCDVYSFGMVLFEIIGRKSNAGTTVSGNPDWFPQH 360

Query: 361 VWDAYENGKLEELIIGCGIGEEDREMASRACEVALWCVQDSPDGRPTMSVVVRMLEGGVE 420
           VWDAYENGKLEELIIGCGIGEEDREMASRACEVALWCVQDSPDGRPTMSVVVRMLEGGVE
Sbjct: 361 VWDAYENGKLEELIIGCGIGEEDREMASRACEVALWCVQDSPDGRPTMSVVVRMLEGGVE 420

Query: 421 IMPPAKPFQYLNPIRTGNSSTSSQQTGSNSSGSSTSRDSNSQWYKKTTTIMRKYEIQMAS 480
           IMPPAKPFQYLNPIRTGNSSTSSQQTGSNSSGSSTSRDSNSQWYKKTTTIMRKYEIQMAS
Sbjct: 421 IMPPAKPFQYLNPIRTGNSSTSSQQTGSNSSGSSTSRDSNSQWYKKTTTIMRKYEIQMAS 480

BLAST of CsGy3G042020 vs. NCBI nr
Match: XP_004136455.2 (rust resistance kinase Lr10 isoform X2 [Cucumis sativus] >KAE8651375.1 hypothetical protein Csa_001388 [Cucumis sativus])

HSP 1 Score: 916 bits (2368), Expect = 0.0
Identity = 458/462 (99.13%), Postives = 460/462 (99.57%), Query Frame = 0

Query: 20  FRSSSSQDNDQVKSITVLWVVGSVVSLVVFVVIVICALKFAKSAAPGIVRELRNITASPV 79
           ++SSSSQDNDQVKSITVLWVVGSVVSLVVFVVIVICALKFAKSAAPGIVRELRNITASPV
Sbjct: 8   YQSSSSQDNDQVKSITVLWVVGSVVSLVVFVVIVICALKFAKSAAPGIVRELRNITASPV 67

Query: 80  VIAAPPVTVPYRTAPPRAASPSLELSRASAAIWRVDAPTMEKFFRKIAEEKPVRFTADQL 139
           VIAAPPVTVPYRTAPPRAASPSLELSRAS AIWRVDAPTMEKFFRKIAEEKPVRFTADQL
Sbjct: 68  VIAAPPVTVPYRTAPPRAASPSLELSRASEAIWRVDAPTMEKFFRKIAEEKPVRFTADQL 127

Query: 140 YTFTSNYSARLGSGGFGEVYKGKFPNGVNIAVKVLKRGSDKRAEEQFMAEVGSICRTYHI 199
           YTFTSNYS RLGSGGFGEVYKGKFPNGVNIAVKVLKRGSDKRAEEQFMAEVGSICRTYHI
Sbjct: 128 YTFTSNYSTRLGSGGFGEVYKGKFPNGVNIAVKVLKRGSDKRAEEQFMAEVGSICRTYHI 187

Query: 200 NLVRLYGFCYDHFMSALVFEYLVNGSLDKYLFGKRKEIEWRKLHDIAIGTAKGLAYLHEE 259
           NLVRLYGFCYDHFMSALVFEYLVNGSLDKYLFGKRKEIEWRKLHDIAIGTAKGLAYLHEE
Sbjct: 188 NLVRLYGFCYDHFMSALVFEYLVNGSLDKYLFGKRKEIEWRKLHDIAIGTAKGLAYLHEE 247

Query: 260 CQQRIIHYDIKPANILLDANFSPKVGDFGLAKLCNRDSTHISFSGYRGTPGYSAPEFLLV 319
           CQQRIIHYDIKPANILLDANFSPKVGDFGLAKLCNRDSTHISFSGYRGTPGYSAPEFLLV
Sbjct: 248 CQQRIIHYDIKPANILLDANFSPKVGDFGLAKLCNRDSTHISFSGYRGTPGYSAPEFLLV 307

Query: 320 NYPLTHKCDVYSFGMVLFEIIGRKSNAGTTVSGNPDWFPQHVWDAYENGKLEELIIGCGI 379
           NYPLTHKCDVYSFGMVLFEIIGRKSNAGTTVSGNPDWFPQHVWDAYENGKLEELIIGCGI
Sbjct: 308 NYPLTHKCDVYSFGMVLFEIIGRKSNAGTTVSGNPDWFPQHVWDAYENGKLEELIIGCGI 367

Query: 380 GEEDREMASRACEVALWCVQDSPDGRPTMSVVVRMLEGGVEIMPPAKPFQYLNPIRTGNS 439
           GEEDREMASRACEVALWCVQDSPDGRPTMSVVVRMLEGGVEIMPPAKPFQYLNPIRTGNS
Sbjct: 368 GEEDREMASRACEVALWCVQDSPDGRPTMSVVVRMLEGGVEIMPPAKPFQYLNPIRTGNS 427

Query: 440 STSSQQTGSNSSGSSTSRDSNSQWYKKTTTIMRKYEIQMASS 481
           STSSQQTGSNSSGSSTSRDSNSQWYKKTTTIMRKYEIQMASS
Sbjct: 428 STSSQQTGSNSSGSSTSRDSNSQWYKKTTTIMRKYEIQMASS 469

BLAST of CsGy3G042020 vs. NCBI nr
Match: XP_008466315.1 (PREDICTED: rust resistance kinase Lr10-like [Cucumis melo])

HSP 1 Score: 865 bits (2235), Expect = 3.21e-315
Identity = 432/456 (94.74%), Postives = 440/456 (96.49%), Query Frame = 0

Query: 27  DNDQVKSITVLWVVGSVVSLVVFVVIVICALKFAKSAAPGIVRELRNITASPVVIAAPPV 86
           D++ VKSIT+LWVVGSVVSLVVFVVI ICALKFAKSAAPG+VRELRNITA PVVIAAPPV
Sbjct: 24  DDNPVKSITILWVVGSVVSLVVFVVIAICALKFAKSAAPGVVRELRNITAPPVVIAAPPV 83

Query: 87  TVPYRTAPPRAASPSLELSRASAAIWRVDAPTMEKFFRKIAEEKPVRFTADQLYTFTSNY 146
           TVPYRTAPPR ASPS ELS ASAAIWRVDAPTMEKFFRKIAEEKPVRFTADQLYTFTSNY
Sbjct: 84  TVPYRTAPPRNASPSPELSTASAAIWRVDAPTMEKFFRKIAEEKPVRFTADQLYTFTSNY 143

Query: 147 SARLGSGGFGEVYKGKFPNGVNIAVKVLKRGSDKRAEEQFMAEVGSICRTYHINLVRLYG 206
           S RLGSGGFGEVYKGKFPNGVN+AVKVLKRGSDKRAEEQFMAEVGSICRTYHINLVRLYG
Sbjct: 144 STRLGSGGFGEVYKGKFPNGVNVAVKVLKRGSDKRAEEQFMAEVGSICRTYHINLVRLYG 203

Query: 207 FCYDHFMSALVFEYLVNGSLDKYLFGKRKEIEWRKLHDIAIGTAKGLAYLHEECQQRIIH 266
           FCYDHFMSALVFEYL NGSLDKYLFGKRKEIEWRKLHDIAIGTAKGLAYLHEECQQRIIH
Sbjct: 204 FCYDHFMSALVFEYLENGSLDKYLFGKRKEIEWRKLHDIAIGTAKGLAYLHEECQQRIIH 263

Query: 267 YDIKPANILLDANFSPKVGDFGLAKLCNRDSTHISFSGYRGTPGYSAPEFLLVNYPLTHK 326
           YDIKPANILLDANF PKVGDFGLAKLCNRDSTHISFSGYRGTPGYSAPEFLLVNYPLTHK
Sbjct: 264 YDIKPANILLDANFFPKVGDFGLAKLCNRDSTHISFSGYRGTPGYSAPEFLLVNYPLTHK 323

Query: 327 CDVYSFGMVLFEIIGRKSNAGTTVSGNPDWFPQHVWDAYENGKLEELIIGCGIGEEDREM 386
           CDVYSFGMVLFEIIGRKSNAG TVSGNPDWFPQHVWDAYENGKLEELI+GCGIGEEDREM
Sbjct: 324 CDVYSFGMVLFEIIGRKSNAGATVSGNPDWFPQHVWDAYENGKLEELIVGCGIGEEDREM 383

Query: 387 ASRACEVALWCVQDSPDGRPTMSVVVRMLEGGVEIMPPAKPFQYLNPIRTGNSSTSS-QQ 446
           ASRACEVALWCVQDSPDGRP MSVVVRMLEGGVEIMPPAKPFQYLNPIR GNSSTSS QQ
Sbjct: 384 ASRACEVALWCVQDSPDGRPPMSVVVRMLEGGVEIMPPAKPFQYLNPIRMGNSSTSSSQQ 443

Query: 447 TGSNSSGSSTSRDSNSQWYKKTTTIMRKYEIQMASS 481
           T S +S SSTSRDSNSQWY+KTTTIMRKYEIQMASS
Sbjct: 444 TSSTTSNSSTSRDSNSQWYRKTTTIMRKYEIQMASS 479

BLAST of CsGy3G042020 vs. NCBI nr
Match: TYK31374.1 (rust resistance kinase Lr10-like [Cucumis melo var. makuwa])

HSP 1 Score: 865 bits (2235), Expect = 9.16e-315
Identity = 432/456 (94.74%), Postives = 440/456 (96.49%), Query Frame = 0

Query: 27  DNDQVKSITVLWVVGSVVSLVVFVVIVICALKFAKSAAPGIVRELRNITASPVVIAAPPV 86
           D++ VKSIT+LWVVGSVVSLVVFVVI ICALKFAKSAAPG+VRELRNITA PVVIAAPPV
Sbjct: 48  DDNPVKSITILWVVGSVVSLVVFVVIAICALKFAKSAAPGVVRELRNITAPPVVIAAPPV 107

Query: 87  TVPYRTAPPRAASPSLELSRASAAIWRVDAPTMEKFFRKIAEEKPVRFTADQLYTFTSNY 146
           TVPYRTAPPR ASPS ELS ASAAIWRVDAPTMEKFFRKIAEEKPVRFTADQLYTFTSNY
Sbjct: 108 TVPYRTAPPRNASPSPELSTASAAIWRVDAPTMEKFFRKIAEEKPVRFTADQLYTFTSNY 167

Query: 147 SARLGSGGFGEVYKGKFPNGVNIAVKVLKRGSDKRAEEQFMAEVGSICRTYHINLVRLYG 206
           S RLGSGGFGEVYKGKFPNGVN+AVKVLKRGSDKRAEEQFMAEVGSICRTYHINLVRLYG
Sbjct: 168 STRLGSGGFGEVYKGKFPNGVNVAVKVLKRGSDKRAEEQFMAEVGSICRTYHINLVRLYG 227

Query: 207 FCYDHFMSALVFEYLVNGSLDKYLFGKRKEIEWRKLHDIAIGTAKGLAYLHEECQQRIIH 266
           FCYDHFMSALVFEYL NGSLDKYLFGKRKEIEWRKLHDIAIGTAKGLAYLHEECQQRIIH
Sbjct: 228 FCYDHFMSALVFEYLENGSLDKYLFGKRKEIEWRKLHDIAIGTAKGLAYLHEECQQRIIH 287

Query: 267 YDIKPANILLDANFSPKVGDFGLAKLCNRDSTHISFSGYRGTPGYSAPEFLLVNYPLTHK 326
           YDIKPANILLDANF PKVGDFGLAKLCNRDSTHISFSGYRGTPGYSAPEFLLVNYPLTHK
Sbjct: 288 YDIKPANILLDANFFPKVGDFGLAKLCNRDSTHISFSGYRGTPGYSAPEFLLVNYPLTHK 347

Query: 327 CDVYSFGMVLFEIIGRKSNAGTTVSGNPDWFPQHVWDAYENGKLEELIIGCGIGEEDREM 386
           CDVYSFGMVLFEIIGRKSNAG TVSGNPDWFPQHVWDAYENGKLEELI+GCGIGEEDREM
Sbjct: 348 CDVYSFGMVLFEIIGRKSNAGATVSGNPDWFPQHVWDAYENGKLEELIVGCGIGEEDREM 407

Query: 387 ASRACEVALWCVQDSPDGRPTMSVVVRMLEGGVEIMPPAKPFQYLNPIRTGNSSTSS-QQ 446
           ASRACEVALWCVQDSPDGRP MSVVVRMLEGGVEIMPPAKPFQYLNPIR GNSSTSS QQ
Sbjct: 408 ASRACEVALWCVQDSPDGRPPMSVVVRMLEGGVEIMPPAKPFQYLNPIRMGNSSTSSSQQ 467

Query: 447 TGSNSSGSSTSRDSNSQWYKKTTTIMRKYEIQMASS 481
           T S +S SSTSRDSNSQWY+KTTTIMRKYEIQMASS
Sbjct: 468 TSSTTSNSSTSRDSNSQWYRKTTTIMRKYEIQMASS 503

BLAST of CsGy3G042020 vs. NCBI nr
Match: XP_038897072.1 (PR5-like receptor kinase isoform X1 [Benincasa hispida])

HSP 1 Score: 823 bits (2126), Expect = 1.38e-298
Identity = 407/457 (89.06%), Postives = 425/457 (93.00%), Query Frame = 0

Query: 25  SQDNDQVKSITVLWVVGSVVSLVVFVVIVICALKFAKSAAPGIVRELRNITASPVVIAAP 84
           S  +D VKS TVL+VVGSVVSLVVFVVI ICA KFAK AAPG+VRELRNITA P+    P
Sbjct: 22  SGGDDPVKSFTVLYVVGSVVSLVVFVVIAICAFKFAKKAAPGVVRELRNITAPPMT--GP 81

Query: 85  PVTVPYRTAPPRAASPSLELSRASAAIWRVDAPTMEKFFRKIAEEKPVRFTADQLYTFTS 144
           P+T PYR APPR ASP  ELS ASAAIWRVDAPTMEKFFRKIAEEKPVRFTADQLYTFTS
Sbjct: 82  PITAPYRPAPPRPASPPPELSMASAAIWRVDAPTMEKFFRKIAEEKPVRFTADQLYTFTS 141

Query: 145 NYSARLGSGGFGEVYKGKFPNGVNIAVKVLKRGSDKRAEEQFMAEVGSICRTYHINLVRL 204
           NYS RLGSGGFGEVYKGKFPNGVN+AVKVLKR SDKRAEEQFMAEVGSICRTYHINLVRL
Sbjct: 142 NYSTRLGSGGFGEVYKGKFPNGVNVAVKVLKRSSDKRAEEQFMAEVGSICRTYHINLVRL 201

Query: 205 YGFCYDHFMSALVFEYLVNGSLDKYLFGKRKEIEWRKLHDIAIGTAKGLAYLHEECQQRI 264
           YGFCYDHFMSALVFEYL NGSLDKYLFGKRKEIEWRKLHDIAIGTA+GLAYLHEECQQRI
Sbjct: 202 YGFCYDHFMSALVFEYLENGSLDKYLFGKRKEIEWRKLHDIAIGTARGLAYLHEECQQRI 261

Query: 265 IHYDIKPANILLDANFSPKVGDFGLAKLCNRDSTHISFSGYRGTPGYSAPEFLLVNYPLT 324
           IHYDIKPANILLDANF PKVGDFGLAKLCNRD+THISFSGYRGTPGYSAPEFLL+NYP+T
Sbjct: 262 IHYDIKPANILLDANFFPKVGDFGLAKLCNRDNTHISFSGYRGTPGYSAPEFLLINYPIT 321

Query: 325 HKCDVYSFGMVLFEIIGRKSNAGTTVSGNPDWFPQHVWDAYENGKLEELIIGCGIGEEDR 384
           HKCDVYSFGMVLFEIIGRKSNA TTVSGNPDWFPQHVWDAYE GKLEELI+GCGIGEED+
Sbjct: 322 HKCDVYSFGMVLFEIIGRKSNADTTVSGNPDWFPQHVWDAYEYGKLEELIVGCGIGEEDK 381

Query: 385 EMASRACEVALWCVQDSPDGRPTMSVVVRMLEGGVEIMPPAKPFQYLNPIRTGNSSTSSQ 444
           E A+RACEVA WCVQDSPDGRP MS VV+MLEGGVEIMPPAKPFQYLNP+R GN+STSSQ
Sbjct: 382 ETANRACEVAFWCVQDSPDGRPPMSAVVKMLEGGVEIMPPAKPFQYLNPMRMGNNSTSSQ 441

Query: 445 QTGSNSSGSSTSRDSNSQWYKKTTTIMRKYEIQMASS 481
           QT S SS SSTSRD+NSQWY+KTTTIMRKYEIQMASS
Sbjct: 442 QTSSTSSDSSTSRDTNSQWYRKTTTIMRKYEIQMASS 476

BLAST of CsGy3G042020 vs. ExPASy TrEMBL
Match: A0A0A0LEH1 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G881760 PE=3 SV=1)

HSP 1 Score: 902 bits (2331), Expect = 0.0
Identity = 457/475 (96.21%), Postives = 459/475 (96.63%), Query Frame = 0

Query: 20  FRSSSSQDNDQVKSITVLW-------------VVGSVVSLVVFVVIVICALKFAKSAAPG 79
           ++SSSSQDNDQVKSITVL              VVGSVVSLVVFVVIVICALKFAKSAAPG
Sbjct: 8   YQSSSSQDNDQVKSITVLCKHTISLISLKLRGVVGSVVSLVVFVVIVICALKFAKSAAPG 67

Query: 80  IVRELRNITASPVVIAAPPVTVPYRTAPPRAASPSLELSRASAAIWRVDAPTMEKFFRKI 139
           IVRELRNITASPVVIAAPPVTVPYRTAPPRAASPSLELSRAS AIWRVDAPTMEKFFRKI
Sbjct: 68  IVRELRNITASPVVIAAPPVTVPYRTAPPRAASPSLELSRASEAIWRVDAPTMEKFFRKI 127

Query: 140 AEEKPVRFTADQLYTFTSNYSARLGSGGFGEVYKGKFPNGVNIAVKVLKRGSDKRAEEQF 199
           AEEKPVRFTADQLYTFTSNYS RLGSGGFGEVYKGKFPNGVNIAVKVLKRGSDKRAEEQF
Sbjct: 128 AEEKPVRFTADQLYTFTSNYSTRLGSGGFGEVYKGKFPNGVNIAVKVLKRGSDKRAEEQF 187

Query: 200 MAEVGSICRTYHINLVRLYGFCYDHFMSALVFEYLVNGSLDKYLFGKRKEIEWRKLHDIA 259
           MAEVGSICRTYHINLVRLYGFCYDHFMSALVFEYLVNGSLDKYLFGKRKEIEWRKLHDIA
Sbjct: 188 MAEVGSICRTYHINLVRLYGFCYDHFMSALVFEYLVNGSLDKYLFGKRKEIEWRKLHDIA 247

Query: 260 IGTAKGLAYLHEECQQRIIHYDIKPANILLDANFSPKVGDFGLAKLCNRDSTHISFSGYR 319
           IGTAKGLAYLHEECQQRIIHYDIKPANILLDANFSPKVGDFGLAKLCNRDSTHISFSGYR
Sbjct: 248 IGTAKGLAYLHEECQQRIIHYDIKPANILLDANFSPKVGDFGLAKLCNRDSTHISFSGYR 307

Query: 320 GTPGYSAPEFLLVNYPLTHKCDVYSFGMVLFEIIGRKSNAGTTVSGNPDWFPQHVWDAYE 379
           GTPGYSAPEFLLVNYPLTHKCDVYSFGMVLFEIIGRKSNAGTTVSGNPDWFPQHVWDAYE
Sbjct: 308 GTPGYSAPEFLLVNYPLTHKCDVYSFGMVLFEIIGRKSNAGTTVSGNPDWFPQHVWDAYE 367

Query: 380 NGKLEELIIGCGIGEEDREMASRACEVALWCVQDSPDGRPTMSVVVRMLEGGVEIMPPAK 439
           NGKLEELIIGCGIGEEDREMASRACEVALWCVQDSPDGRPTMSVVVRMLEGGVEIMPPAK
Sbjct: 368 NGKLEELIIGCGIGEEDREMASRACEVALWCVQDSPDGRPTMSVVVRMLEGGVEIMPPAK 427

Query: 440 PFQYLNPIRTGNSSTSSQQTGSNSSGSSTSRDSNSQWYKKTTTIMRKYEIQMASS 481
           PFQYLNPIRTGNSSTSSQQTGSNSSGSSTSRDSNSQWYKKTTTIMRKYEIQMASS
Sbjct: 428 PFQYLNPIRTGNSSTSSQQTGSNSSGSSTSRDSNSQWYKKTTTIMRKYEIQMASS 482

BLAST of CsGy3G042020 vs. ExPASy TrEMBL
Match: A0A1S3CQY1 (rust resistance kinase Lr10-like OS=Cucumis melo OX=3656 GN=LOC103503761 PE=3 SV=1)

HSP 1 Score: 865 bits (2235), Expect = 1.55e-315
Identity = 432/456 (94.74%), Postives = 440/456 (96.49%), Query Frame = 0

Query: 27  DNDQVKSITVLWVVGSVVSLVVFVVIVICALKFAKSAAPGIVRELRNITASPVVIAAPPV 86
           D++ VKSIT+LWVVGSVVSLVVFVVI ICALKFAKSAAPG+VRELRNITA PVVIAAPPV
Sbjct: 24  DDNPVKSITILWVVGSVVSLVVFVVIAICALKFAKSAAPGVVRELRNITAPPVVIAAPPV 83

Query: 87  TVPYRTAPPRAASPSLELSRASAAIWRVDAPTMEKFFRKIAEEKPVRFTADQLYTFTSNY 146
           TVPYRTAPPR ASPS ELS ASAAIWRVDAPTMEKFFRKIAEEKPVRFTADQLYTFTSNY
Sbjct: 84  TVPYRTAPPRNASPSPELSTASAAIWRVDAPTMEKFFRKIAEEKPVRFTADQLYTFTSNY 143

Query: 147 SARLGSGGFGEVYKGKFPNGVNIAVKVLKRGSDKRAEEQFMAEVGSICRTYHINLVRLYG 206
           S RLGSGGFGEVYKGKFPNGVN+AVKVLKRGSDKRAEEQFMAEVGSICRTYHINLVRLYG
Sbjct: 144 STRLGSGGFGEVYKGKFPNGVNVAVKVLKRGSDKRAEEQFMAEVGSICRTYHINLVRLYG 203

Query: 207 FCYDHFMSALVFEYLVNGSLDKYLFGKRKEIEWRKLHDIAIGTAKGLAYLHEECQQRIIH 266
           FCYDHFMSALVFEYL NGSLDKYLFGKRKEIEWRKLHDIAIGTAKGLAYLHEECQQRIIH
Sbjct: 204 FCYDHFMSALVFEYLENGSLDKYLFGKRKEIEWRKLHDIAIGTAKGLAYLHEECQQRIIH 263

Query: 267 YDIKPANILLDANFSPKVGDFGLAKLCNRDSTHISFSGYRGTPGYSAPEFLLVNYPLTHK 326
           YDIKPANILLDANF PKVGDFGLAKLCNRDSTHISFSGYRGTPGYSAPEFLLVNYPLTHK
Sbjct: 264 YDIKPANILLDANFFPKVGDFGLAKLCNRDSTHISFSGYRGTPGYSAPEFLLVNYPLTHK 323

Query: 327 CDVYSFGMVLFEIIGRKSNAGTTVSGNPDWFPQHVWDAYENGKLEELIIGCGIGEEDREM 386
           CDVYSFGMVLFEIIGRKSNAG TVSGNPDWFPQHVWDAYENGKLEELI+GCGIGEEDREM
Sbjct: 324 CDVYSFGMVLFEIIGRKSNAGATVSGNPDWFPQHVWDAYENGKLEELIVGCGIGEEDREM 383

Query: 387 ASRACEVALWCVQDSPDGRPTMSVVVRMLEGGVEIMPPAKPFQYLNPIRTGNSSTSS-QQ 446
           ASRACEVALWCVQDSPDGRP MSVVVRMLEGGVEIMPPAKPFQYLNPIR GNSSTSS QQ
Sbjct: 384 ASRACEVALWCVQDSPDGRPPMSVVVRMLEGGVEIMPPAKPFQYLNPIRMGNSSTSSSQQ 443

Query: 447 TGSNSSGSSTSRDSNSQWYKKTTTIMRKYEIQMASS 481
           T S +S SSTSRDSNSQWY+KTTTIMRKYEIQMASS
Sbjct: 444 TSSTTSNSSTSRDSNSQWYRKTTTIMRKYEIQMASS 479

BLAST of CsGy3G042020 vs. ExPASy TrEMBL
Match: A0A5D3E5X7 (Rust resistance kinase Lr10-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G006810 PE=4 SV=1)

HSP 1 Score: 865 bits (2235), Expect = 4.44e-315
Identity = 432/456 (94.74%), Postives = 440/456 (96.49%), Query Frame = 0

Query: 27  DNDQVKSITVLWVVGSVVSLVVFVVIVICALKFAKSAAPGIVRELRNITASPVVIAAPPV 86
           D++ VKSIT+LWVVGSVVSLVVFVVI ICALKFAKSAAPG+VRELRNITA PVVIAAPPV
Sbjct: 48  DDNPVKSITILWVVGSVVSLVVFVVIAICALKFAKSAAPGVVRELRNITAPPVVIAAPPV 107

Query: 87  TVPYRTAPPRAASPSLELSRASAAIWRVDAPTMEKFFRKIAEEKPVRFTADQLYTFTSNY 146
           TVPYRTAPPR ASPS ELS ASAAIWRVDAPTMEKFFRKIAEEKPVRFTADQLYTFTSNY
Sbjct: 108 TVPYRTAPPRNASPSPELSTASAAIWRVDAPTMEKFFRKIAEEKPVRFTADQLYTFTSNY 167

Query: 147 SARLGSGGFGEVYKGKFPNGVNIAVKVLKRGSDKRAEEQFMAEVGSICRTYHINLVRLYG 206
           S RLGSGGFGEVYKGKFPNGVN+AVKVLKRGSDKRAEEQFMAEVGSICRTYHINLVRLYG
Sbjct: 168 STRLGSGGFGEVYKGKFPNGVNVAVKVLKRGSDKRAEEQFMAEVGSICRTYHINLVRLYG 227

Query: 207 FCYDHFMSALVFEYLVNGSLDKYLFGKRKEIEWRKLHDIAIGTAKGLAYLHEECQQRIIH 266
           FCYDHFMSALVFEYL NGSLDKYLFGKRKEIEWRKLHDIAIGTAKGLAYLHEECQQRIIH
Sbjct: 228 FCYDHFMSALVFEYLENGSLDKYLFGKRKEIEWRKLHDIAIGTAKGLAYLHEECQQRIIH 287

Query: 267 YDIKPANILLDANFSPKVGDFGLAKLCNRDSTHISFSGYRGTPGYSAPEFLLVNYPLTHK 326
           YDIKPANILLDANF PKVGDFGLAKLCNRDSTHISFSGYRGTPGYSAPEFLLVNYPLTHK
Sbjct: 288 YDIKPANILLDANFFPKVGDFGLAKLCNRDSTHISFSGYRGTPGYSAPEFLLVNYPLTHK 347

Query: 327 CDVYSFGMVLFEIIGRKSNAGTTVSGNPDWFPQHVWDAYENGKLEELIIGCGIGEEDREM 386
           CDVYSFGMVLFEIIGRKSNAG TVSGNPDWFPQHVWDAYENGKLEELI+GCGIGEEDREM
Sbjct: 348 CDVYSFGMVLFEIIGRKSNAGATVSGNPDWFPQHVWDAYENGKLEELIVGCGIGEEDREM 407

Query: 387 ASRACEVALWCVQDSPDGRPTMSVVVRMLEGGVEIMPPAKPFQYLNPIRTGNSSTSS-QQ 446
           ASRACEVALWCVQDSPDGRP MSVVVRMLEGGVEIMPPAKPFQYLNPIR GNSSTSS QQ
Sbjct: 408 ASRACEVALWCVQDSPDGRPPMSVVVRMLEGGVEIMPPAKPFQYLNPIRMGNSSTSSSQQ 467

Query: 447 TGSNSSGSSTSRDSNSQWYKKTTTIMRKYEIQMASS 481
           T S +S SSTSRDSNSQWY+KTTTIMRKYEIQMASS
Sbjct: 468 TSSTTSNSSTSRDSNSQWYRKTTTIMRKYEIQMASS 503

BLAST of CsGy3G042020 vs. ExPASy TrEMBL
Match: A0A6J1C5Q2 (rust resistance kinase Lr10-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111008202 PE=3 SV=1)

HSP 1 Score: 712 bits (1838), Expect = 2.41e-255
Identity = 360/473 (76.11%), Postives = 391/473 (82.66%), Query Frame = 0

Query: 16  LFCNFRSSSSQD----NDQVKSITVLWVVGSVVSLVVFVVIVICALKFAKSAAPGIVREL 75
           L C F    + D     +   ++  L+VVGSV+SL+V   I I A KFAK+A PG+VREL
Sbjct: 6   LLCQFSPPDAPDFGGGGNPANTLVALYVVGSVISLLVLAGIGISAYKFAKAATPGVVREL 65

Query: 76  RNITASPVVIAAPPVTVPYRTAPPRAASPSLELSRASAAIWRVDAPTMEKFFRKIAEEKP 135
           RNI   P     PPV  P               + A A  W VDAPTMEKFF++IAEEKP
Sbjct: 66  RNINTPP-----PPVPFP---------------NMAPAVPWEVDAPTMEKFFQEIAEEKP 125

Query: 136 VRFTADQLYTFTSNYSARLGSGGFGEVYKGKFPNGVNIAVKVLKRGSDKRAEEQFMAEVG 195
           VRFTA QLY FTSNYS RLGSGGFGEVYKGKFPNGVNIAVKVLK+ SDKRAEEQFMAE+G
Sbjct: 126 VRFTAQQLYNFTSNYSTRLGSGGFGEVYKGKFPNGVNIAVKVLKKSSDKRAEEQFMAEIG 185

Query: 196 SICRTYHINLVRLYGFCYDHFMSALVFEYLVNGSLDKYLFGKRKEIEWRKLHDIAIGTAK 255
           SICRTYHINLVRLYGFCYDHFMSALVFEY+ NGSLDKYLFGKR+EIEW KLHDIAIGTA+
Sbjct: 186 SICRTYHINLVRLYGFCYDHFMSALVFEYMDNGSLDKYLFGKRQEIEWGKLHDIAIGTAR 245

Query: 256 GLAYLHEECQQRIIHYDIKPANILLDANFSPKVGDFGLAKLCNRDSTHISFSGYRGTPGY 315
           GLAYLHEEC QRIIHYDIKPANILLD NF PKVGDFGLAKLCNRD THISFSGYRGTPGY
Sbjct: 246 GLAYLHEECHQRIIHYDIKPANILLDNNFFPKVGDFGLAKLCNRDITHISFSGYRGTPGY 305

Query: 316 SAPEFLLVNYPLTHKCDVYSFGMVLFEIIGRKSNAGTTVSGNPDWFPQHVWDAYENGKLE 375
            APEFL+ NYP+THKCDVYSFGMVLFEIIGRKSNAGTT+SGNPDWFPQHVWDAYENGKL 
Sbjct: 306 MAPEFLMTNYPITHKCDVYSFGMVLFEIIGRKSNAGTTISGNPDWFPQHVWDAYENGKLA 365

Query: 376 ELIIGCGIGEEDREMASRACEVALWCVQDSPDGRPTMSVVVRMLEGGVEIMPPAKPFQYL 435
           ELI+ CGIGEEDRE AS+ACEVALWCVQDSPDGRP MS VV+MLEGGVEIMPP KPFQYL
Sbjct: 366 ELIVACGIGEEDREKASKACEVALWCVQDSPDGRPPMSAVVKMLEGGVEIMPPPKPFQYL 425

Query: 436 N--PIRTGNSSTSSQQTGSNS-SGSSTSRDSNSQWYKKTTTIMRKYEIQMASS 481
           N  P + G S+TSS +T S++ S SS SR+SNSQWYKKTTT+MRKYEIQMA+S
Sbjct: 426 NSFPRKLGTSTTSSGETNSSTGSDSSASRESNSQWYKKTTTVMRKYEIQMATS 458

BLAST of CsGy3G042020 vs. ExPASy TrEMBL
Match: A0A6J1C3R4 (rust resistance kinase Lr10-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111008202 PE=3 SV=1)

HSP 1 Score: 673 bits (1737), Expect = 3.01e-241
Identity = 325/378 (85.98%), Postives = 346/378 (91.53%), Query Frame = 0

Query: 107 ASAAIWRVDAPTMEKFFRKIAEEKPVRFTADQLYTFTSNYSARLGSGGFGEVYKGKFPNG 166
           A A  W VDAPTMEKFF++IAEEKPVRFTA QLY FTSNYS RLGSGGFGEVYKGKFPNG
Sbjct: 2   APAVPWEVDAPTMEKFFQEIAEEKPVRFTAQQLYNFTSNYSTRLGSGGFGEVYKGKFPNG 61

Query: 167 VNIAVKVLKRGSDKRAEEQFMAEVGSICRTYHINLVRLYGFCYDHFMSALVFEYLVNGSL 226
           VNIAVKVLK+ SDKRAEEQFMAE+GSICRTYHINLVRLYGFCYDHFMSALVFEY+ NGSL
Sbjct: 62  VNIAVKVLKKSSDKRAEEQFMAEIGSICRTYHINLVRLYGFCYDHFMSALVFEYMDNGSL 121

Query: 227 DKYLFGKRKEIEWRKLHDIAIGTAKGLAYLHEECQQRIIHYDIKPANILLDANFSPKVGD 286
           DKYLFGKR+EIEW KLHDIAIGTA+GLAYLHEEC QRIIHYDIKPANILLD NF PKVGD
Sbjct: 122 DKYLFGKRQEIEWGKLHDIAIGTARGLAYLHEECHQRIIHYDIKPANILLDNNFFPKVGD 181

Query: 287 FGLAKLCNRDSTHISFSGYRGTPGYSAPEFLLVNYPLTHKCDVYSFGMVLFEIIGRKSNA 346
           FGLAKLCNRD THISFSGYRGTPGY APEFL+ NYP+THKCDVYSFGMVLFEIIGRKSNA
Sbjct: 182 FGLAKLCNRDITHISFSGYRGTPGYMAPEFLMTNYPITHKCDVYSFGMVLFEIIGRKSNA 241

Query: 347 GTTVSGNPDWFPQHVWDAYENGKLEELIIGCGIGEEDREMASRACEVALWCVQDSPDGRP 406
           GTT+SGNPDWFPQHVWDAYENGKL ELI+ CGIGEEDRE AS+ACEVALWCVQDSPDGRP
Sbjct: 242 GTTISGNPDWFPQHVWDAYENGKLAELIVACGIGEEDREKASKACEVALWCVQDSPDGRP 301

Query: 407 TMSVVVRMLEGGVEIMPPAKPFQYLN--PIRTGNSSTSSQQTGSNS-SGSSTSRDSNSQW 466
            MS VV+MLEGGVEIMPP KPFQYLN  P + G S+TSS +T S++ S SS SR+SNSQW
Sbjct: 302 PMSAVVKMLEGGVEIMPPPKPFQYLNSFPRKLGTSTTSSGETNSSTGSDSSASRESNSQW 361

Query: 467 YKKTTTIMRKYEIQMASS 481
           YKKTTT+MRKYEIQMA+S
Sbjct: 362 YKKTTTVMRKYEIQMATS 379

BLAST of CsGy3G042020 vs. TAIR 10
Match: AT5G38280.1 (PR5-like receptor kinase )

HSP 1 Score: 259.6 bits (662), Expect = 4.9e-69
Identity = 131/300 (43.67%), Postives = 193/300 (64.33%), Query Frame = 0

Query: 133 RFTADQLYTFTSNYSARLGSGGFGEVYKGKF-PNGVNIAVKVLKRGSDKRAEEQFMAEVG 192
           R++  ++   T++++  LG GGFG VYKGK   +G ++AVK+LK        E+F+ EV 
Sbjct: 320 RYSYTRVKKMTNSFAHVLGKGGFGTVYKGKLADSGRDVAVKILKVSEGN--GEEFINEVA 379

Query: 193 SICRTYHINLVRLYGFCYDHFMSALVFEYLVNGSLDKYLFGKRK-EIEWRKLHDIAIGTA 252
           S+ RT H+N+V L GFCY+    A+++E++ NGSLDKY+      ++EW +L+D+A+G +
Sbjct: 380 SMSRTSHVNIVSLLGFCYEKNKRAIIYEFMPNGSLDKYISANMSTKMEWERLYDVAVGIS 439

Query: 253 KGLAYLHEECQQRIIHYDIKPANILLDANFSPKVGDFGLAKLCNRDSTHISFSGYRGTPG 312
           +GL YLH  C  RI+H+DIKP NIL+D N  PK+ DFGLAKLC    + IS    RGT G
Sbjct: 440 RGLEYLHNRCVTRIVHFDIKPQNILMDENLCPKISDFGLAKLCKNKESIISMLHMRGTFG 499

Query: 313 YSAPEFLLVNY-PLTHKCDVYSFGMVLFEIIGRKSNAGTTVSGNPD---WFPQHVWDAYE 372
           Y APE    N+  ++HK DVYS+GMV+ E+IG K+      SG+ +   +FP+ V+  +E
Sbjct: 500 YIAPEMFSKNFGAVSHKSDVYSYGMVVLEMIGAKNIEKVEYSGSNNGSMYFPEWVYKDFE 559

Query: 373 NGKLEELIIGCGIGEEDREMASRACEVALWCVQDSPDGRPTMSVVVRMLEGGVEIM--PP 425
            G++   I G  I +E+ ++A +   VALWC+Q +P  RP M  V+ MLEG +E +  PP
Sbjct: 560 KGEITR-IFGDSITDEEEKIAKKLVLVALWCIQMNPSDRPPMIKVIEMLEGNLEALQVPP 616

BLAST of CsGy3G042020 vs. TAIR 10
Match: AT1G70250.1 (receptor serine/threonine kinase, putative )

HSP 1 Score: 254.2 bits (648), Expect = 2.1e-67
Identity = 134/311 (43.09%), Postives = 189/311 (60.77%), Query Frame = 0

Query: 133 RFTADQLYTFTSNYSARLGSGGFGEVYKGKFPNGV-NIAVKVLKRGSDKRAEEQFMAEVG 192
           RF+  Q+   T ++   LG GGFG VYKGK P+G  ++AVK+LK  ++    E F+ E+ 
Sbjct: 448 RFSYVQVKKMTKSFENVLGKGGFGTVYKGKLPDGSRDVAVKILKESNED--GEDFINEIA 507

Query: 193 SICRTYHINLVRLYGFCYDHFMSALVFEYLVNGSLDKYLF-GKRKEIEWRKLHDIAIGTA 252
           S+ RT H N+V L GFCY+    A+++E + NGSLDK++      ++EW+ L++IA+G +
Sbjct: 508 SMSRTSHANIVSLLGFCYEGRKKAIIYELMPNGSLDKFISKNMSAKMEWKTLYNIAVGVS 567

Query: 253 KGLAYLHEECQQRIIHYDIKPANILLDANFSPKVGDFGLAKLCNRDSTHISFSGYRGTPG 312
            GL YLH  C  RI+H+DIKP NIL+D +  PK+ DFGLAKLC  + + IS    RGT G
Sbjct: 568 HGLEYLHSHCVSRIVHFDIKPQNILIDGDLCPKISDFGLAKLCKNNESIISMLHARGTIG 627

Query: 313 YSAPEFLLVNY-PLTHKCDVYSFGMVLFEI-----IGRKSNAGTTVSGNPDWFPQHVWDA 372
           Y APE    N+  ++HK DVYS+GMV+ E+     IGR  NAG+  S    +FP  ++  
Sbjct: 628 YIAPEVFSQNFGGVSHKSDVYSYGMVVLEMIGARNIGRAQNAGS--SNTSMYFPDWIYKD 687

Query: 373 YENGKLEELIIGCGIGEEDREMASRACEVALWCVQDSPDGRPTMSVVVRMLEGGVEIMP- 432
            E G++   +      EED ++  +   V LWC+Q +P  RP MS VV MLEG +E +  
Sbjct: 688 LEKGEIMSFLADQITEEEDEKIVKKMVLVGLWCIQTNPYDRPPMSKVVEMLEGSLEALQI 747

Query: 433 PAKPFQYLNPI 435
           P KP   L  I
Sbjct: 748 PPKPLLCLPAI 754

BLAST of CsGy3G042020 vs. TAIR 10
Match: AT5G24080.1 (Protein kinase superfamily protein )

HSP 1 Score: 253.4 bits (646), Expect = 3.5e-67
Identity = 136/313 (43.45%), Postives = 186/313 (59.42%), Query Frame = 0

Query: 129 EKPVRFTADQLYTFTSNYSARLGSGGFGEVYKGKFPNGVNIAVKVLKRGSDKRAEEQFMA 188
           + PV FT   L   T+N+S  LGSGGFG VYKG       +AVK L R      E +F+ 
Sbjct: 113 DSPVSFTYRDLQNCTNNFSQLLGSGGFGTVYKGTVAGETLVAVKRLDRAL-SHGEREFIT 172

Query: 189 EVGSICRTYHINLVRLYGFCYDHFMSALVFEYLVNGSLDKYLFGKRKE---IEWRKLHDI 248
           EV +I   +H+NLVRL G+C +     LV+EY++NGSLDK++F   +    ++WR   +I
Sbjct: 173 EVNTIGSMHHMNLVRLCGYCSEDSHRLLVYEYMINGSLDKWIFSSEQTANLLDWRTRFEI 232

Query: 249 AIGTAKGLAYLHEECQQRIIHYDIKPANILLDANFSPKVGDFGLAKLCNRDSTHISFSGY 308
           A+ TA+G+AY HE+C+ RIIH DIKP NILLD NF PKV DFGLAK+  R+ +H+  +  
Sbjct: 233 AVATAQGIAYFHEQCRNRIIHCDIKPENILLDDNFCPKVSDFGLAKMMGREHSHV-VTMI 292

Query: 309 RGTPGYSAPEFLLVNYPLTHKCDVYSFGMVLFEIIGRKSNAGTTVSGNPDWFPQHVWDAY 368
           RGT GY APE+ + N P+T K DVYS+GM+L EI+G + N   +      ++P   +   
Sbjct: 293 RGTRGYLAPEW-VSNRPITVKADVYSYGMLLLEIVGGRRNLDMSYDAEDFFYPGWAYKEL 352

Query: 369 ENGKLEELIIGCGIGEEDREMASRACEVALWCVQDSPDGRPTMSVVVRMLEG-GVEIMPP 428
            NG   + +     G  + E   +A +VA WC+QD    RP+M  VV++LEG   EI  P
Sbjct: 353 TNGTSLKAVDKRLQGVAEEEEVVKALKVAFWCIQDEVSMRPSMGEVVKLLEGTSDEINLP 412

Query: 429 AKPFQYLNPIRTG 438
             P   L  I  G
Sbjct: 413 PMPQTILELIEEG 422

BLAST of CsGy3G042020 vs. TAIR 10
Match: AT4G32300.1 (S-domain-2 5 )

HSP 1 Score: 253.1 bits (645), Expect = 4.6e-67
Identity = 144/339 (42.48%), Postives = 204/339 (60.18%), Query Frame = 0

Query: 131 PVRFTADQLYTFTSNYSARLGSGGFGEVYKGKFPNGVNIAVKVLKRGSDKRAEEQFMAEV 190
           P+RF    L + T+N+S +LG GGFG VY+G  P+G  +AVK L+     + +++F AEV
Sbjct: 480 PIRFAYKDLQSATNNFSVKLGQGGFGSVYEGTLPDGSRLAVKKLE--GIGQGKKEFRAEV 539

Query: 191 GSICRTYHINLVRLYGFCYDHFMSALVFEYLVNGSLDKYLFGKRKE---IEWRKLHDIAI 250
             I   +H++LVRL GFC +     L +E+L  GSL++++F K+     ++W    +IA+
Sbjct: 540 SIIGSIHHLHLVRLRGFCAEGAHRLLAYEFLSKGSLERWIFRKKDGDVLLDWDTRFNIAL 599

Query: 251 GTAKGLAYLHEECQQRIIHYDIKPANILLDANFSPKVGDFGLAKLCNRDSTHISFSGYRG 310
           GTAKGLAYLHE+C  RI+H DIKP NILLD NF+ KV DFGLAKL  R+ +H+ F+  RG
Sbjct: 600 GTAKGLAYLHEDCDARIVHCDIKPENILLDDNFNAKVSDFGLAKLMTREQSHV-FTTMRG 659

Query: 311 TPGYSAPEFLLVNYPLTHKCDVYSFGMVLFEIIGRKSNAGTTVSGNPDWFPQHVWDAYEN 370
           T GY APE+ + NY ++ K DVYS+GMVL E+IG + N   + +     FP   +   E 
Sbjct: 660 TRGYLAPEW-ITNYAISEKSDVYSYGMVLLELIGGRKNYDPSETSEKCHFPSFAFKKMEE 719

Query: 371 GKLEELIIG--CGIGEEDREMASRACEVALWCVQDSPDGRPTMSVVVRMLEGGVEIMPPA 430
           GKL +++ G    +   D E   RA + ALWC+Q+    RP+MS VV+MLEG   ++ P 
Sbjct: 720 GKLMDIVDGKMKNVDVTD-ERVQRAMKTALWCIQEDMQTRPSMSKVVQMLEGVFPVVQP- 779

Query: 431 KPFQYLNPIRTGNSSTSSQQTGSNSSGSSTSRDSNSQWY 465
            P       R  +S   S      ++ SS   D NS+ Y
Sbjct: 780 -PSSSTMGSRLYSSFFKSISEDGGATTSSGPSDCNSENY 811

BLAST of CsGy3G042020 vs. TAIR 10
Match: AT1G34300.1 (lectin protein kinase family protein )

HSP 1 Score: 251.9 bits (642), Expect = 1.0e-66
Identity = 143/335 (42.69%), Postives = 195/335 (58.21%), Query Frame = 0

Query: 127 AEEKPVRFTADQLYTFTSNYSARLGSGGFGEVYKGKFPNGVNIAVKVLKRGSDKRAEEQF 186
           A   PV+FT  +L   T ++  +LG+GGFG VY+G   N   +AVK L+    ++ E+QF
Sbjct: 467 ASGAPVQFTYKELQRCTKSFKEKLGAGGFGTVYRGVLTNRTVVAVKQLE--GIEQGEKQF 526

Query: 187 MAEVGSICRTYHINLVRLYGFCYDHFMSALVFEYLVNGSLDKYLF--GKRKEIEWRKLHD 246
             EV +I  T+H+NLVRL GFC       LV+E++ NGSLD +LF     K + W    +
Sbjct: 527 RMEVATISSTHHLNLVRLIGFCSQGRHRLLVYEFMRNGSLDNFLFTTDSAKFLTWEYRFN 586

Query: 247 IAIGTAKGLAYLHEECQQRIIHYDIKPANILLDANFSPKVGDFGLAKLCNRDSTHISFSG 306
           IA+GTAKG+ YLHEEC+  I+H DIKP NIL+D NF+ KV DFGLAKL N      + S 
Sbjct: 587 IALGTAKGITYLHEECRDCIVHCDIKPENILVDDNFAAKVSDFGLAKLLNPKDNRYNMSS 646

Query: 307 YRGTPGYSAPEFLLVNYPLTHKCDVYSFGMVLFEIIGRKSNAGTTVSGNPDWFPQHVWDA 366
            RGT GY APE+ L N P+T K DVYS+GMVL E++  K N   +   N   F    ++ 
Sbjct: 647 VRGTRGYLAPEW-LANLPITSKSDVYSYGMVLLELVSGKRNFDVSEKTNHKKFSIWAYEE 706

Query: 367 YENGKLEELIIGCGIGEE---DREMASRACEVALWCVQDSPDGRPTMSVVVRMLEGGVEI 426
           +E G   + I+   + E+   D E   R  + + WC+Q+ P  RPTM  VV+MLEG  EI
Sbjct: 707 FEKGN-TKAILDTRLSEDQTVDMEQVMRMVKTSFWCIQEQPLQRPTMGKVVQMLEGITEI 766

Query: 427 MPPAKPFQYLNPIRTGNSSTSSQQTGSNSSGSSTS 457
             P  P        +GNS ++S  +   +SG + S
Sbjct: 767 KNPLCPKTISEVSFSGNSMSTSHASMFVASGPTRS 797

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P936041.8e-6841.19Rust resistance kinase Lr10 OS=Triticum aestivum OX=4565 GN=LRK10 PE=2 SV=1[more]
Q9FF296.9e-6843.67PR5-like receptor kinase OS=Arabidopsis thaliana OX=3702 GN=PR5K PE=1 SV=1[more]
Q9FLV45.0e-6643.45G-type lectin S-receptor-like serine/threonine-protein kinase At5g24080 OS=Arabi... [more]
Q8RWZ56.5e-6642.48G-type lectin S-receptor-like serine/threonine-protein kinase SD2-5 OS=Arabidops... [more]
Q9XID31.4e-6542.69G-type lectin S-receptor-like serine/threonine-protein kinase At1g34300 OS=Arabi... [more]
Match NameE-valueIdentityDescription
XP_031738771.10.099.38rust resistance kinase Lr10 isoform X1 [Cucumis sativus][more]
XP_004136455.20.099.13rust resistance kinase Lr10 isoform X2 [Cucumis sativus] >KAE8651375.1 hypotheti... [more]
XP_008466315.13.21e-31594.74PREDICTED: rust resistance kinase Lr10-like [Cucumis melo][more]
TYK31374.19.16e-31594.74rust resistance kinase Lr10-like [Cucumis melo var. makuwa][more]
XP_038897072.11.38e-29889.06PR5-like receptor kinase isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A0A0LEH10.096.21Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G881... [more]
A0A1S3CQY11.55e-31594.74rust resistance kinase Lr10-like OS=Cucumis melo OX=3656 GN=LOC103503761 PE=3 SV... [more]
A0A5D3E5X74.44e-31594.74Rust resistance kinase Lr10-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A6J1C5Q22.41e-25576.11rust resistance kinase Lr10-like isoform X1 OS=Momordica charantia OX=3673 GN=LO... [more]
A0A6J1C3R43.01e-24185.98rust resistance kinase Lr10-like isoform X2 OS=Momordica charantia OX=3673 GN=LO... [more]
Match NameE-valueIdentityDescription
AT5G38280.14.9e-6943.67PR5-like receptor kinase [more]
AT1G70250.12.1e-6743.09receptor serine/threonine kinase, putative [more]
AT5G24080.13.5e-6743.45Protein kinase superfamily protein [more]
AT4G32300.14.6e-6742.48S-domain-2 5 [more]
AT1G34300.11.0e-6642.69lectin protein kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 144..415
e-value: 1.4E-25
score: 101.0
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 144..429
score: 37.050175
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 147..415
e-value: 3.8E-44
score: 150.9
NoneNo IPR availablePIRSFPIRSF000615TyrPK_CSF1-Rcoord: 383..479
e-value: 1.6
score: 4.6
coord: 145..247
e-value: 0.0016
score: 14.5
coord: 238..347
e-value: 4.5E-8
score: 29.6
coord: 18..61
e-value: 210.0
score: -2.4
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 118..220
e-value: 3.4E-24
score: 86.9
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 221..422
e-value: 7.9E-55
score: 187.4
NoneNo IPR availablePIRSRPIRSR037921-1PIRSR037921-1coord: 150..345
e-value: 1.1E-18
score: 65.5
NoneNo IPR availablePIRSRPIRSR000620-1PIRSR000620-1coord: 148..419
e-value: 1.6E-15
score: 54.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 430..464
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 432..464
NoneNo IPR availablePANTHERPTHR27009:SF80RECEPTOR SERINE/THREONINE KINASEcoord: 115..477
NoneNo IPR availablePANTHERPTHR27009RUST RESISTANCE KINASE LR10-RELATEDcoord: 115..477
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 264..276
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 150..172
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 126..415

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy3G042020.2CsGy3G042020.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity