CsGy3G036830 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy3G036830
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionPectate lyase
LocationGy14Chr3: 34865860 .. 34867453 (-)
RNA-Seq ExpressionCsGy3G036830
SyntenyCsGy3G036830
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGACAGGTTGTAAAATTAAAGTTGTGATGGTTTTGGTTTCAATGGCTGTTCTTATGCCAACTCTTAGAGCTGGTATTGCTGAGTTTGATGATTTTTTGCTCCAGAAGTCTGAAGAAGCTAAACAAGCTTCTCTTGAAGCTTTCCACCCTGACCCCATGAATGTCACTGACCATTTCAATCAACATGTTCATCTGTATGTTTATAATATATAATCTTTCTACTTCACTTCTGACTACTCATTTGGATGGTATTCTATTGACATTGACGTTTTGTTTTGTATGTAGAGCTTTGGAGGGGATAGAGGGAAGCAATAGCACCAGGAGGAGCCTATCCAAATACAACGGGCCCTGCTTGGCTACAAATCCAATTGATCGTTGCTGGCGTTGTGACCCAAATTGGGCCAAGAACCGTAAGAAGCTTGCCAAATGTGTGCTTGGTTTCGGCAGAAAAACCACTGGTGGCAAAAAGGGACGTATCTACGTTGTGACTGATCCATCAGATAACGATGTTATTAATCCTAGGCCTGGAACCCTTAGATATGGCGCTCTTCAAAAGAAACCTTTATGGATCATTTTTGCGCGTAGCATGATTATTAGGCTATCCAAGGAGCTGATGATTACTAGCCACAAGACAATCGATGCTCGTGGTGCCAATGTTCACATTGCCTACGGTGCTGGTCTTAGCATTCAATTCGCTCGGAATGTAATCATCCATGGCCTTCGCATCCACCATGTCATCTCAACTGGCGGTGGCATGATCCGCGACACTGCCAACCATGTCGGTCTTCGAACAGTTAGTGATGGAGACGGTATCTCCATCTTTGGATCAACCAACATCTGGCTCGATCATCTCTCCATGTCTAATTGCCAAGATGGACTTATCGACGCAATCCAAGGCTCTACTGCAATCACCATCTCAAACTCTCATTTCACTCATCACAATGATGTAAACTATATGATTCAAATTACTTCTTGCACATACTCTTTAAATCTTTCAAAAACATAACTTAATACTCTCCATTTCATTACACAGGTGATGCTGTTTGGTGCAAGTGATTCATATCAAGGCGACTCGATCATGCAAGTGACCGTCGCATTCAACCATTTTGGAAAGGGATTAGTGCAGAGAATGCCAAGATGCAGATGGGGTTTCTTCCATGTCGTCAACAATGATTACACTCACTGGCTCATGTACGCCATTGGTGGCAGTCAACATCCCACCATTATCAGTCAAGGAAACCGTTTCATTGCCCCACCCAACCCTGCTGCACGACAGGTAAAAAAAAAAAAAACCAAGACAGTCTAATAGGCTCTGATACCCTAACAAAATGAAATTCATTAACGAATAATTTTTGTATACATGCAGATCACAAATAGAAACTATGCAACAGAGAGTGTATGGAAAACATGGACATGGAGATCGGAAGGGGATTTGATGATGAATGGAGCTTATTTCGTTCAGTCCGGGAATCCAAGCAAACGAAGACCATACAGCAGGTTCGACATGATAAAGGCTAAGCCTGGCACTTTTGTTAGAAGGCTGACTCGCTTCTCTGGCTCACTCAACTGTTATGTTGGTCGCCCATGCTAA

mRNA sequence

ATGATGACAGGTTGTAAAATTAAAGTTGTGATGGTTTTGGTTTCAATGGCTGTTCTTATGCCAACTCTTAGAGCTGGTATTGCTGAGTTTGATGATTTTTTGCTCCAGAAGTCTGAAGAAGCTAAACAAGCTTCTCTTGAAGCTTTCCACCCTGACCCCATGAATGTCACTGACCATTTCAATCAACATGTTCATCTAGCTTTGGAGGGGATAGAGGGAAGCAATAGCACCAGGAGGAGCCTATCCAAATACAACGGGCCCTGCTTGGCTACAAATCCAATTGATCGTTGCTGGCGTTGTGACCCAAATTGGGCCAAGAACCGTAAGAAGCTTGCCAAATGTGTGCTTGGTTTCGGCAGAAAAACCACTGGTGGCAAAAAGGGACGTATCTACGTTGTGACTGATCCATCAGATAACGATGTTATTAATCCTAGGCCTGGAACCCTTAGATATGGCGCTCTTCAAAAGAAACCTTTATGGATCATTTTTGCGCGTAGCATGATTATTAGGCTATCCAAGGAGCTGATGATTACTAGCCACAAGACAATCGATGCTCGTGGTGCCAATGTTCACATTGCCTACGGTGCTGGTCTTAGCATTCAATTCGCTCGGAATGTAATCATCCATGGCCTTCGCATCCACCATGTCATCTCAACTGGCGGTGGCATGATCCGCGACACTGCCAACCATGTCGGTCTTCGAACAGTTAGTGATGGAGACGGTATCTCCATCTTTGGATCAACCAACATCTGGCTCGATCATCTCTCCATGTCTAATTGCCAAGATGGACTTATCGACGCAATCCAAGGCTCTACTGCAATCACCATCTCAAACTCTCATTTCACTCATCACAATGATGTGATGCTGTTTGGTGCAAGTGATTCATATCAAGGCGACTCGATCATGCAAGTGACCGTCGCATTCAACCATTTTGGAAAGGGATTAGTGCAGAGAATGCCAAGATGCAGATGGGGTTTCTTCCATGTCGTCAACAATGATTACACTCACTGGCTCATGTACGCCATTGGTGGCAGTCAACATCCCACCATTATCAGTCAAGGAAACCGTTTCATTGCCCCACCCAACCCTGCTGCACGACAGATCACAAATAGAAACTATGCAACAGAGAGTGTATGGAAAACATGGACATGGAGATCGGAAGGGGATTTGATGATGAATGGAGCTTATTTCGTTCAGTCCGGGAATCCAAGCAAACGAAGACCATACAGCAGGTTCGACATGATAAAGGCTAAGCCTGGCACTTTTGTTAGAAGGCTGACTCGCTTCTCTGGCTCACTCAACTGTTATGTTGGTCGCCCATGCTAA

Coding sequence (CDS)

ATGATGACAGGTTGTAAAATTAAAGTTGTGATGGTTTTGGTTTCAATGGCTGTTCTTATGCCAACTCTTAGAGCTGGTATTGCTGAGTTTGATGATTTTTTGCTCCAGAAGTCTGAAGAAGCTAAACAAGCTTCTCTTGAAGCTTTCCACCCTGACCCCATGAATGTCACTGACCATTTCAATCAACATGTTCATCTAGCTTTGGAGGGGATAGAGGGAAGCAATAGCACCAGGAGGAGCCTATCCAAATACAACGGGCCCTGCTTGGCTACAAATCCAATTGATCGTTGCTGGCGTTGTGACCCAAATTGGGCCAAGAACCGTAAGAAGCTTGCCAAATGTGTGCTTGGTTTCGGCAGAAAAACCACTGGTGGCAAAAAGGGACGTATCTACGTTGTGACTGATCCATCAGATAACGATGTTATTAATCCTAGGCCTGGAACCCTTAGATATGGCGCTCTTCAAAAGAAACCTTTATGGATCATTTTTGCGCGTAGCATGATTATTAGGCTATCCAAGGAGCTGATGATTACTAGCCACAAGACAATCGATGCTCGTGGTGCCAATGTTCACATTGCCTACGGTGCTGGTCTTAGCATTCAATTCGCTCGGAATGTAATCATCCATGGCCTTCGCATCCACCATGTCATCTCAACTGGCGGTGGCATGATCCGCGACACTGCCAACCATGTCGGTCTTCGAACAGTTAGTGATGGAGACGGTATCTCCATCTTTGGATCAACCAACATCTGGCTCGATCATCTCTCCATGTCTAATTGCCAAGATGGACTTATCGACGCAATCCAAGGCTCTACTGCAATCACCATCTCAAACTCTCATTTCACTCATCACAATGATGTGATGCTGTTTGGTGCAAGTGATTCATATCAAGGCGACTCGATCATGCAAGTGACCGTCGCATTCAACCATTTTGGAAAGGGATTAGTGCAGAGAATGCCAAGATGCAGATGGGGTTTCTTCCATGTCGTCAACAATGATTACACTCACTGGCTCATGTACGCCATTGGTGGCAGTCAACATCCCACCATTATCAGTCAAGGAAACCGTTTCATTGCCCCACCCAACCCTGCTGCACGACAGATCACAAATAGAAACTATGCAACAGAGAGTGTATGGAAAACATGGACATGGAGATCGGAAGGGGATTTGATGATGAATGGAGCTTATTTCGTTCAGTCCGGGAATCCAAGCAAACGAAGACCATACAGCAGGTTCGACATGATAAAGGCTAAGCCTGGCACTTTTGTTAGAAGGCTGACTCGCTTCTCTGGCTCACTCAACTGTTATGTTGGTCGCCCATGCTAA

Protein sequence

MMTGCKIKVVMVLVSMAVLMPTLRAGIAEFDDFLLQKSEEAKQASLEAFHPDPMNVTDHFNQHVHLALEGIEGSNSTRRSLSKYNGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGRKTTGGKKGRIYVVTDPSDNDVINPRPGTLRYGALQKKPLWIIFARSMIIRLSKELMITSHKTIDARGANVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGDGISIFGSTNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDSIMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNPAARQITNRNYATESVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSRFDMIKAKPGTFVRRLTRFSGSLNCYVGRPC*
Homology
BLAST of CsGy3G036830 vs. ExPASy Swiss-Prot
Match: Q9SRH4 (Probable pectate lyase 7 OS=Arabidopsis thaliana OX=3702 GN=At3g01270 PE=2 SV=1)

HSP 1 Score: 578.2 bits (1489), Expect = 8.1e-164
Identity = 281/476 (59.03%), Postives = 349/476 (73.32%), Query Frame = 0

Query: 1   MMTGCKIKVVMVLVSMAVLMPTLRAGIA-EFDDFLLQKSEEAKQASLEAFHPDPMNVTDH 60
           M T    K+V V + +A L+PT+RA +A E D++ + K+ EA++ +L A+HPDP  + DH
Sbjct: 1   METARLFKLVCV-ICIASLIPTIRANVADETDEYWVNKANEARKHTLMAYHPDPYEIVDH 60

Query: 61  FNQHVHLALEGIEG---------------------SNSTRRSL------------SKYNG 120
           F++  +     +EG                     +NSTRRSL            SK  G
Sbjct: 61  FHERHYDNSTDVEGTEEEKAVASEEEDVIEMISSPTNSTRRSLTGRGKGKGKGKWSKLTG 120

Query: 121 PCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGRKTTGGKKGRIYVVTDPSDNDVINPRP 180
           PC A+NPID+CWRC P+WA+ RKKL  CV GFG +TTGGK+GRIYVVT P D+D++NPRP
Sbjct: 121 PCTASNPIDKCWRCQPDWARRRKKLVHCVRGFGYRTTGGKRGRIYVVTSPRDDDMVNPRP 180

Query: 181 GTLRYGALQKKPLWIIFARSMIIRLSKELMITSHKTIDARGANVHIAYGAGLSIQFARNV 240
           GTLR+  +QK+PLWI+F   M IRLS+ELMITS KTIDARGANVHIAYGAG+++Q+  N+
Sbjct: 181 GTLRHAVIQKEPLWIVFKHDMSIRLSQELMITSDKTIDARGANVHIAYGAGITMQYVHNI 240

Query: 241 IIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGDGISIFGSTNIWLDHLSMSNCQDGLID 300
           IIHGL +HH++ + GG+IRD+ NH G R  +DGDGISIFG+TNIWLDH+SMS CQDGLID
Sbjct: 241 IIHGLHVHHIVKSSGGLIRDSINHFGHRGEADGDGISIFGATNIWLDHISMSKCQDGLID 300

Query: 301 AIQGSTAITISNSHFTHHNDVMLFGASDSYQGDSIMQVTVAFNHFGKGLVQRMPRCRWGF 360
           AI GSTAITISNSHFTHHNDVML GA ++   D  MQVTVA+NHFGKGLVQRMPR RWGF
Sbjct: 301 AIMGSTAITISNSHFTHHNDVMLLGAQNNNMDDKKMQVTVAYNHFGKGLVQRMPRVRWGF 360

Query: 361 FHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNPA-ARQITNRNYATESVWKTWTWR 420
            HVVNNDYTHW +YAIGGSQ PTI+S GNRFIAPP+    R++T R+YA+ES WK W WR
Sbjct: 361 VHVVNNDYTHWELYAIGGSQGPTILSHGNRFIAPPHKQHYREVTKRDYASESEWKNWNWR 420

Query: 421 SEGDLMMNGAYFVQSGNPSKRRPYSRFDMIKAKPGTFVRRLTRFSGSLNCYVGRPC 442
           SE D+ MN AYF QSGNP  +  +SR  MIK K G  V +LT+++G+L+C VG+ C
Sbjct: 421 SEKDVFMNNAYFRQSGNPHFKCSHSRQQMIKPKNGMAVSKLTKYAGALDCRVGKAC 475

BLAST of CsGy3G036830 vs. ExPASy Swiss-Prot
Match: Q9LFP5 (Probable pectate lyase 19 OS=Arabidopsis thaliana OX=3702 GN=At5g15110 PE=2 SV=1)

HSP 1 Score: 573.9 bits (1478), Expect = 1.5e-162
Identity = 273/468 (58.33%), Postives = 348/468 (74.36%), Query Frame = 0

Query: 6   KIKVVMVLVSMAVLMPTLRAGIAEFDDFLLQKSEEAKQASLEAFHPDPMNVTDHFNQHVH 65
           ++  +M    +AVL+PT+R  I+E D++  Q+++EA++ +L+A+H DP  + DHF++  +
Sbjct: 5   RLSKLMFTFCIAVLIPTIRGNISELDEYWSQRADEAREFTLQAYHSDPYEIVDHFHERHY 64

Query: 66  -------------------LALEGIE----GSNSTRRSL--------SKYNGPCLATNPI 125
                                 E IE     +NSTRRSL        SK  GPC A+NPI
Sbjct: 65  DNSTDVTTPEEDGDAKPEEEEKEFIEMLGSSTNSTRRSLRGKGKGKWSKLKGPCTASNPI 124

Query: 126 DRCWRCDPNWAKNRKKLAKCVLGFGRKTTGGKKGRIYVVTDPSDNDVINPRPGTLRYGAL 185
           D+CWRC  +WAK RKKL +CV GFG +TTGGK+GRIYVVT   D D++NP+PGTLR+  +
Sbjct: 125 DKCWRCRSDWAKRRKKLTRCVRGFGHRTTGGKRGRIYVVTSNLDEDMVNPKPGTLRHAVI 184

Query: 186 QKKPLWIIFARSMIIRLSKELMITSHKTIDARGANVHIAYGAGLSIQFARNVIIHGLRIH 245
           QK+PLWIIF   M IRL++EL+I SHKTIDARGANVH+A+GAG+++QF +NVIIHGL IH
Sbjct: 185 QKEPLWIIFKNDMSIRLNQELLINSHKTIDARGANVHVAHGAGITMQFVKNVIIHGLHIH 244

Query: 246 HVISTGGGMIRDTANHVGLRTVSDGDGISIFGSTNIWLDHLSMSNCQDGLIDAIQGSTAI 305
           H+  + GGMIRD+ +H G+RT +DGDG+SI+GS+NIWLDH+SMS CQDGLIDAI GST I
Sbjct: 245 HISESSGGMIRDSVDHFGMRTRADGDGLSIYGSSNIWLDHISMSKCQDGLIDAIVGSTGI 304

Query: 306 TISNSHFTHHNDVMLFGASDSYQGDSIMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDY 365
           TISNSHFTHHNDVML GA ++ + D  MQVTVA+NHFGKGLVQRMPR RWGF HVVNNDY
Sbjct: 305 TISNSHFTHHNDVMLLGAQNTNEADKHMQVTVAYNHFGKGLVQRMPRIRWGFVHVVNNDY 364

Query: 366 THWLMYAIGGSQHPTIISQGNRFIAPPN-PAARQITNRNYATESVWKTWTWRSEGDLMMN 425
           THW +YAIGGSQ PTI+S GNRFIAPP+ P  R++T R+YA+E  WK W WRS+ D+ MN
Sbjct: 365 THWELYAIGGSQGPTILSHGNRFIAPPHKPHYREVTKRDYASEDEWKHWNWRSDKDVFMN 424

Query: 426 GAYFVQSGNPSKRRPYSRFDMIKAKPGTFVRRLTRFSGSLNCYVGRPC 442
           GAYF QSGNP  +  ++R  MIK K G  V +LT+++G+L+C VGR C
Sbjct: 425 GAYFRQSGNPQYKCAHTRQQMIKPKNGLAVSKLTKYAGALDCRVGRRC 472

BLAST of CsGy3G036830 vs. ExPASy Swiss-Prot
Match: P40973 (Pectate lyase OS=Lilium longiflorum OX=4690 PE=2 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 3.5e-159
Identity = 266/418 (63.64%), Postives = 328/418 (78.47%), Query Frame = 0

Query: 25  AGIAEFDDFLLQKSEEAKQASLEAFHPDPMNVTDHFNQHVHLALEGIEGSNSTRRSL-SK 84
           A IAEFD++  +KS+ A+  + +A+ P P  VT+HFN+ VH + EG    NSTRR+L + 
Sbjct: 22  ANIAEFDEYWQKKSKVAQAKAKKAYTPHPEEVTNHFNKAVHSSFEG----NSTRRNLRTN 81

Query: 85  YNGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGRKTTGGKKGRIYVVTDPSDNDVIN 144
             G CLATNPIDRCWRC  NW+ NRK L KCV GFGRKTTGG  G IYVVTDPSD+ + +
Sbjct: 82  KLGQCLATNPIDRCWRCKKNWSANRKDLVKCVKGFGRKTTGGAAGEIYVVTDPSDDSLTD 141

Query: 145 PRPGTLRYGALQKKPLWIIFARSMIIRLSKELMITSHKTIDARGANVHIAYGAGLSIQFA 204
           P+ GTLR+G +Q +PLWIIF +SM+IRL +EL+I + KTID RGANV IA GA L++QF 
Sbjct: 142 PKFGTLRWGVIQDRPLWIIFGKSMVIRLKQELIINNDKTIDGRGANVQIAGGAQLTVQFV 201

Query: 205 RNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGDGISIFGSTNIWLDHLSMSNCQDG 264
            NVIIHG+ IH +    GG+IRD+  H G+RT SDGDGISI GS+NIW+DH+S++ C DG
Sbjct: 202 HNVIIHGIHIHDIKPGEGGLIRDSEKHSGIRTRSDGDGISIIGSSNIWIDHVSLARCSDG 261

Query: 265 LIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDSIMQVTVAFNHFGKGLVQRMPRCR 324
           LID I GSTAITISN H T H+DVML GASD+Y  D IMQVTVAFNHFG+GLVQRMPRCR
Sbjct: 262 LIDVILGSTAITISNCHLTEHDDVMLLGASDTYTQDEIMQVTVAFNHFGRGLVQRMPRCR 321

Query: 325 WGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNPAARQITNRNYATESVWKTWT 384
           +GF HVVNNDYTHW+MYA+GGSQHPTIISQGNR+IAP   AA+++T R+YA  + W  WT
Sbjct: 322 YGFVHVVNNDYTHWIMYAVGGSQHPTIISQGNRYIAPHIEAAKEVTKRDYAEPAEWSKWT 381

Query: 385 WRSEGDLMMNGAYFVQSGNPSKRRPYSRFDMIKAKPGTFVRRLTRFSGSLNCYVGRPC 442
           W+S+GDL ++GA+FV+SG P + + YS+ D+IKAKPGTFV+RLTRFSG+LNC     C
Sbjct: 382 WKSQGDLFVSGAFFVESGGPFENK-YSKKDLIKAKPGTFVQRLTRFSGALNCKENMEC 434

BLAST of CsGy3G036830 vs. ExPASy Swiss-Prot
Match: Q9M9S2 (Probable pectate lyase 3 OS=Arabidopsis thaliana OX=3702 GN=AT59 PE=2 SV=1)

HSP 1 Score: 556.2 bits (1432), Expect = 3.3e-157
Identity = 262/440 (59.55%), Postives = 323/440 (73.41%), Query Frame = 0

Query: 16  MAVLMPTLRAGIAEFDDFLLQKSEEAKQASLEAFHPDPMNVTDHFNQHVHLALEGIEGSN 75
           +A++ P +R  +A FD +  Q+  +A + ++ ++ P P+NVT+HFN HV++A++  E  N
Sbjct: 20  LAIVAPQVRGNVAVFDSYWTQRQSDALKQTIGSYDPHPLNVTNHFNYHVNIAVDASESRN 79

Query: 76  STRRSLS---------KYNGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGRKTTGGK 135
            TRR L+         K +G CLA NPID CWRCD NWA NRKKLA CVLGFGR+TTGGK
Sbjct: 80  DTRRELTQVRSGRKTHKSSGKCLAYNPIDNCWRCDRNWANNRKKLADCVLGFGRRTTGGK 139

Query: 136 KGRIYVVTDPSDNDVINPRPGTLRYGALQKKPLWIIFARSMIIRLSKELMITSHKTIDAR 195
            G IYVV D SDND+INP+PGTLR+   +  PLWIIFARSMII+L +ELMITS KTID R
Sbjct: 140 DGPIYVVKDASDNDLINPKPGTLRHAVTRDGPLWIIFARSMIIKLQQELMITSDKTIDGR 199

Query: 196 GANVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGDGISIFG 255
           GA V+I  GAGL++QF  NVIIH + + H++   GG+IRD+  H+GLRT SDGDGIS+FG
Sbjct: 200 GARVYIMEGAGLTLQFVNNVIIHNIYVKHIVPGNGGLIRDSEAHIGLRTKSDGDGISLFG 259

Query: 256 STNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDSIMQVTV 315
           +TNIW+DH+SM+ C DG+IDAI GSTA+TISNSHFT H +VMLFGA D +  D  MQ+TV
Sbjct: 260 ATNIWIDHVSMTRCADGMIDAIDGSTAVTISNSHFTDHQEVMLFGARDEHVIDKKMQITV 319

Query: 316 AFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNPAAR 375
           AFNHFGK L QRMPRCR+G  HVVNNDYTHW MYAIGG+ +PTIISQGNRFIAPPN  A+
Sbjct: 320 AFNHFGKRLEQRMPRCRYGTIHVVNNDYTHWEMYAIGGNMNPTIISQGNRFIAPPNEEAK 379

Query: 376 QITNRNYATESVWKTWTWRSEGDLMMNGAYFVQSG-----NPSKRRPYSRFDMIKAKPGT 435
           QIT R Y     WK+W W+SEGD  +NGAYFVQSG     +   + P      I+ KPGT
Sbjct: 380 QITKREYTPYGEWKSWNWQSEGDYFLNGAYFVQSGKANAWSSKPKTPLPNKFTIRPKPGT 439

Query: 436 FVRRLTRFSGSLNCYVGRPC 442
            VR+LT  +G L C +G  C
Sbjct: 440 MVRKLTMDAGVLGCKLGEAC 459

BLAST of CsGy3G036830 vs. ExPASy Swiss-Prot
Match: P15722 (Probable pectate lyase P59 OS=Solanum lycopersicum OX=4081 GN=LAT59 PE=2 SV=1)

HSP 1 Score: 548.5 bits (1412), Expect = 6.9e-155
Identity = 263/450 (58.44%), Postives = 329/450 (73.11%), Query Frame = 0

Query: 2   MTGCKIK--VVMVLVSMAVLMPTLRAGIAEFDDFLLQKSEEAKQASLEAFHPDPMNVTDH 61
           M G KIK   + + ++ A ++P+L A I  +D+   +++EEAK+ +   + P P NVT  
Sbjct: 1   MGGPKIKYSFLFLCITFATIIPSLMAHIGHYDEVWRRRAEEAKEYARNIYEPHPENVTLA 60

Query: 62  FNQHVH---LALEGIEG--SNSTRRSL--SKYNGPCLATNPIDRCWRCDPNWAKNRKKLA 121
           FNQ +      L+ ++G  +NSTRR L   KY GPC+ TNPID+CWRCDPNWA NRKKLA
Sbjct: 61  FNQKLRDTMKELKKVKGTHNNSTRRGLGTKKYTGPCMVTNPIDKCWRCDPNWADNRKKLA 120

Query: 122 KCVLGFGRKTTGGKKGRIYVVTDPSDNDVINPRPGTLRYGALQKKPLWIIFARSMIIRLS 181
            C +GFG K  GGK G  YVVTD SD D  +P+PGTLR+  +QK+PLWIIF R M IRL 
Sbjct: 121 DCAMGFGSKAIGGKDGEFYVVTDNSD-DYNDPKPGTLRHAVIQKEPLWIIFKRGMNIRLH 180

Query: 182 KELMITSHKTIDARGANVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVG 241
           +E+++ S KTIDARG NVHI  GAG+++Q+ +NVIIHGL IH ++   GGM+RD  +H+G
Sbjct: 181 QEMIMQSDKTIDARGVNVHITKGAGITLQYIKNVIIHGLHIHDIVEGNGGMVRDAVDHIG 240

Query: 242 LRTVSDGDGISIFGSTNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGA 301
           +RT SDGDGISIFG++ IW+DH+SM  C DGLIDA++GST ITISN HFT HN+VMLFGA
Sbjct: 241 IRTKSDGDGISIFGASYIWIDHVSMQRCYDGLIDAVEGSTGITISNGHFTDHNEVMLFGA 300

Query: 302 SDSYQGDSIMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIIS 361
           SDS   D +MQ+T+AFNHFGK L+QRMPRCRWG+ HVVNNDYTHW MYAIGGS HPTII 
Sbjct: 301 SDSSSIDQVMQITLAFNHFGKRLIQRMPRCRWGYIHVVNNDYTHWNMYAIGGSMHPTIIH 360

Query: 362 QGNRFIAPPNPAARQITNRNYATESVWKTWTWRSEGDLMMNGAYFVQSGNPS-KRRPYSR 421
           QGNRFIAPP+   +Q+T R Y  ESVW  WTWRSEG+L MNGAYF +SG+P    +    
Sbjct: 361 QGNRFIAPPDIFKKQVTKREYNPESVWMQWTWRSEGNLFMNGAYFTESGDPEWSSKHKDL 420

Query: 422 FDMIKAKPGTFVRRLTRFSGSLNCYVGRPC 442
           +D I A P   V  +TRF+G L C  G+PC
Sbjct: 421 YDGISAAPAEDVTWMTRFAGVLGCKPGKPC 449

BLAST of CsGy3G036830 vs. NCBI nr
Match: XP_004147462.2 (probable pectate lyase 7 [Cucumis sativus] >KGN59593.1 hypothetical protein Csa_001450 [Cucumis sativus])

HSP 1 Score: 912 bits (2358), Expect = 0.0
Identity = 441/441 (100.00%), Postives = 441/441 (100.00%), Query Frame = 0

Query: 1   MMTGCKIKVVMVLVSMAVLMPTLRAGIAEFDDFLLQKSEEAKQASLEAFHPDPMNVTDHF 60
           MMTGCKIKVVMVLVSMAVLMPTLRAGIAEFDDFLLQKSEEAKQASLEAFHPDPMNVTDHF
Sbjct: 1   MMTGCKIKVVMVLVSMAVLMPTLRAGIAEFDDFLLQKSEEAKQASLEAFHPDPMNVTDHF 60

Query: 61  NQHVHLALEGIEGSNSTRRSLSKYNGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGR 120
           NQHVHLALEGIEGSNSTRRSLSKYNGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGR
Sbjct: 61  NQHVHLALEGIEGSNSTRRSLSKYNGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGR 120

Query: 121 KTTGGKKGRIYVVTDPSDNDVINPRPGTLRYGALQKKPLWIIFARSMIIRLSKELMITSH 180
           KTTGGKKGRIYVVTDPSDNDVINPRPGTLRYGALQKKPLWIIFARSMIIRLSKELMITSH
Sbjct: 121 KTTGGKKGRIYVVTDPSDNDVINPRPGTLRYGALQKKPLWIIFARSMIIRLSKELMITSH 180

Query: 181 KTIDARGANVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGD 240
           KTIDARGANVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGD
Sbjct: 181 KTIDARGANVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGD 240

Query: 241 GISIFGSTNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS 300
           GISIFGSTNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS
Sbjct: 241 GISIFGSTNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS 300

Query: 301 IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 360
           IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP
Sbjct: 301 IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 360

Query: 361 PNPAARQITNRNYATESVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSRFDMIKAKPG 420
           PNPAARQITNRNYATESVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSRFDMIKAKPG
Sbjct: 361 PNPAARQITNRNYATESVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSRFDMIKAKPG 420

Query: 421 TFVRRLTRFSGSLNCYVGRPC 441
           TFVRRLTRFSGSLNCYVGRPC
Sbjct: 421 TFVRRLTRFSGSLNCYVGRPC 441

BLAST of CsGy3G036830 vs. NCBI nr
Match: XP_008443432.1 (PREDICTED: probable pectate lyase 19 [Cucumis melo])

HSP 1 Score: 891 bits (2302), Expect = 0.0
Identity = 424/441 (96.15%), Postives = 438/441 (99.32%), Query Frame = 0

Query: 1   MMTGCKIKVVMVLVSMAVLMPTLRAGIAEFDDFLLQKSEEAKQASLEAFHPDPMNVTDHF 60
           M+ GCKIK++MVLVSMAVLMPTLRAGIAEFDD+LLQK+EEAKQASLEAFHPDPMNVTDHF
Sbjct: 1   MVAGCKIKLLMVLVSMAVLMPTLRAGIAEFDDYLLQKAEEAKQASLEAFHPDPMNVTDHF 60

Query: 61  NQHVHLALEGIEGSNSTRRSLSKYNGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGR 120
           NQHVHLALEGIEGSNSTRRSL+KY GPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGR
Sbjct: 61  NQHVHLALEGIEGSNSTRRSLAKYKGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGR 120

Query: 121 KTTGGKKGRIYVVTDPSDNDVINPRPGTLRYGALQKKPLWIIFARSMIIRLSKELMITSH 180
           KTTGGKKGRIYVVTDPSDNDV+NP+PGTLRYGALQK+PLWIIFARSMIIRLSKELM+TSH
Sbjct: 121 KTTGGKKGRIYVVTDPSDNDVVNPKPGTLRYGALQKRPLWIIFARSMIIRLSKELMVTSH 180

Query: 181 KTIDARGANVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGD 240
           KTIDARG+NVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGD
Sbjct: 181 KTIDARGSNVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGD 240

Query: 241 GISIFGSTNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS 300
           GISIFGS+NIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS
Sbjct: 241 GISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS 300

Query: 301 IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 360
           IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP
Sbjct: 301 IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 360

Query: 361 PNPAARQITNRNYATESVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSRFDMIKAKPG 420
           PNPAARQITNRNYA ESVWKTWTWRSEGDLMMNGAYF+QSGNPSKRRPYSRFDMIKAKPG
Sbjct: 361 PNPAARQITNRNYAPESVWKTWTWRSEGDLMMNGAYFIQSGNPSKRRPYSRFDMIKAKPG 420

Query: 421 TFVRRLTRFSGSLNCYVGRPC 441
           TFVRRLTRFSGSLNC+VGRPC
Sbjct: 421 TFVRRLTRFSGSLNCFVGRPC 441

BLAST of CsGy3G036830 vs. NCBI nr
Match: KAA0053742.1 (putative pectate lyase 19 [Cucumis melo var. makuwa])

HSP 1 Score: 850 bits (2197), Expect = 1.13e-310
Identity = 406/422 (96.21%), Postives = 419/422 (99.29%), Query Frame = 0

Query: 1   MMTGCKIKVVMVLVSMAVLMPTLRAGIAEFDDFLLQKSEEAKQASLEAFHPDPMNVTDHF 60
           M+ GCKIK++MVLVSMAVLMPTLRAGIAEFDD+LLQK+EEAKQASLEAFHPDPMNVTDHF
Sbjct: 1   MVAGCKIKLLMVLVSMAVLMPTLRAGIAEFDDYLLQKAEEAKQASLEAFHPDPMNVTDHF 60

Query: 61  NQHVHLALEGIEGSNSTRRSLSKYNGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGR 120
           NQHVHLALEGIEGSNSTRRSL+KY GPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGR
Sbjct: 61  NQHVHLALEGIEGSNSTRRSLAKYKGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGR 120

Query: 121 KTTGGKKGRIYVVTDPSDNDVINPRPGTLRYGALQKKPLWIIFARSMIIRLSKELMITSH 180
           KTTGGKKGRIYVVTDPSDNDV+NP+PGTLRYGALQK+PLWIIFARSMIIRLSKELM+TSH
Sbjct: 121 KTTGGKKGRIYVVTDPSDNDVVNPKPGTLRYGALQKRPLWIIFARSMIIRLSKELMVTSH 180

Query: 181 KTIDARGANVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGD 240
           KTIDARG+NVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGD
Sbjct: 181 KTIDARGSNVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGD 240

Query: 241 GISIFGSTNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS 300
           GISIFGS+NIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS
Sbjct: 241 GISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS 300

Query: 301 IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 360
           IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP
Sbjct: 301 IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 360

Query: 361 PNPAARQITNRNYATESVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSRFDMIKAKPG 420
           PNPAARQITNRNYA ESVWKTWTWRSEGDLMMNGAYF+QSGNPSKRRPYSRFDMIKAKPG
Sbjct: 361 PNPAARQITNRNYAPESVWKTWTWRSEGDLMMNGAYFIQSGNPSKRRPYSRFDMIKAKPG 420

Query: 421 TF 422
           TF
Sbjct: 421 TF 422

BLAST of CsGy3G036830 vs. NCBI nr
Match: XP_038905811.1 (pectate lyase-like [Benincasa hispida])

HSP 1 Score: 850 bits (2196), Expect = 1.61e-310
Identity = 402/437 (91.99%), Postives = 422/437 (96.57%), Query Frame = 0

Query: 5   CKIKVVMVLVSMAVLMPTLRAGIAEFDDFLLQKSEEAKQASLEAFHPDPMNVTDHFNQHV 64
           C IKVVM LVSMAVLMPTLRAGIAE+DD+LLQK+EEAKQA+LE+FHPDPMNVTDHFN+HV
Sbjct: 5   CSIKVVMFLVSMAVLMPTLRAGIAEYDDYLLQKAEEAKQAALESFHPDPMNVTDHFNEHV 64

Query: 65  HLALEGIEGSNSTRRSLSKYNGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGRKTTG 124
           HLALEGIEGSNSTRRSL KYNGPC+ATNPIDRCWRCD NWA NRKKL  CVLGFGRKTTG
Sbjct: 65  HLALEGIEGSNSTRRSLKKYNGPCMATNPIDRCWRCDRNWAMNRKKLVSCVLGFGRKTTG 124

Query: 125 GKKGRIYVVTDPSDNDVINPRPGTLRYGALQKKPLWIIFARSMIIRLSKELMITSHKTID 184
           G +GR YVVTDPSDNDVINPR GTLR+ A+QK+PLWIIF RSMIIRLSKEL+ITSHKTID
Sbjct: 125 GLRGRFYVVTDPSDNDVINPRRGTLRHAAIQKRPLWIIFGRSMIIRLSKELLITSHKTID 184

Query: 185 ARGANVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGDGISI 244
           ARGANVHIAYGAGLSIQF+RNVIIHGL IHHV+S  GGMIRD+A+HVGLRTVSDGDGISI
Sbjct: 185 ARGANVHIAYGAGLSIQFSRNVIIHGLHIHHVVSARGGMIRDSADHVGLRTVSDGDGISI 244

Query: 245 FGSTNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDSIMQV 304
           FGS+NIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDSIMQV
Sbjct: 245 FGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDSIMQV 304

Query: 305 TVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNPA 364
           TVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNPA
Sbjct: 305 TVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNPA 364

Query: 365 ARQITNRNYATESVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSRFDMIKAKPGTFVR 424
           ARQ+TNRNYATESVW+TWTWRSEGDLMMNGAYF+QSGNPSKRRPYSRFDMIKAKPGTFVR
Sbjct: 365 ARQVTNRNYATESVWRTWTWRSEGDLMMNGAYFIQSGNPSKRRPYSRFDMIKAKPGTFVR 424

Query: 425 RLTRFSGSLNCYVGRPC 441
           RLTRFSGSLNCYVGRPC
Sbjct: 425 RLTRFSGSLNCYVGRPC 441

BLAST of CsGy3G036830 vs. NCBI nr
Match: TYK25661.1 (putative pectate lyase 19 [Cucumis melo var. makuwa])

HSP 1 Score: 828 bits (2138), Expect = 3.69e-302
Identity = 395/411 (96.11%), Postives = 408/411 (99.27%), Query Frame = 0

Query: 1   MMTGCKIKVVMVLVSMAVLMPTLRAGIAEFDDFLLQKSEEAKQASLEAFHPDPMNVTDHF 60
           M+ GCKIK++MVLVSMAVLMPTLRAGIAEFDD+LLQK+EEAKQASLEAFHPDPMNVTDHF
Sbjct: 1   MVAGCKIKLLMVLVSMAVLMPTLRAGIAEFDDYLLQKAEEAKQASLEAFHPDPMNVTDHF 60

Query: 61  NQHVHLALEGIEGSNSTRRSLSKYNGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGR 120
           NQHVHLALEGIEGSNSTRRSL+KY GPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGR
Sbjct: 61  NQHVHLALEGIEGSNSTRRSLAKYKGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGR 120

Query: 121 KTTGGKKGRIYVVTDPSDNDVINPRPGTLRYGALQKKPLWIIFARSMIIRLSKELMITSH 180
           KTTGGKKGRIYVVTDPSDNDV+NP+PGTLRYGALQK+PLWIIFARSMIIRLSKELM+TSH
Sbjct: 121 KTTGGKKGRIYVVTDPSDNDVVNPKPGTLRYGALQKRPLWIIFARSMIIRLSKELMVTSH 180

Query: 181 KTIDARGANVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGD 240
           KTIDARG+NVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGD
Sbjct: 181 KTIDARGSNVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGD 240

Query: 241 GISIFGSTNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS 300
           GISIFGS+NIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS
Sbjct: 241 GISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS 300

Query: 301 IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 360
           IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP
Sbjct: 301 IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 360

Query: 361 PNPAARQITNRNYATESVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSR 411
           PNPAARQITNRNYA ESVWKTWTWRSEGDLMMNGAYF+QSGNPSKRRPYSR
Sbjct: 361 PNPAARQITNRNYAPESVWKTWTWRSEGDLMMNGAYFIQSGNPSKRRPYSR 411

BLAST of CsGy3G036830 vs. ExPASy TrEMBL
Match: A0A0A0LCU3 (Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_3G827350 PE=3 SV=1)

HSP 1 Score: 912 bits (2358), Expect = 0.0
Identity = 441/441 (100.00%), Postives = 441/441 (100.00%), Query Frame = 0

Query: 1   MMTGCKIKVVMVLVSMAVLMPTLRAGIAEFDDFLLQKSEEAKQASLEAFHPDPMNVTDHF 60
           MMTGCKIKVVMVLVSMAVLMPTLRAGIAEFDDFLLQKSEEAKQASLEAFHPDPMNVTDHF
Sbjct: 1   MMTGCKIKVVMVLVSMAVLMPTLRAGIAEFDDFLLQKSEEAKQASLEAFHPDPMNVTDHF 60

Query: 61  NQHVHLALEGIEGSNSTRRSLSKYNGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGR 120
           NQHVHLALEGIEGSNSTRRSLSKYNGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGR
Sbjct: 61  NQHVHLALEGIEGSNSTRRSLSKYNGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGR 120

Query: 121 KTTGGKKGRIYVVTDPSDNDVINPRPGTLRYGALQKKPLWIIFARSMIIRLSKELMITSH 180
           KTTGGKKGRIYVVTDPSDNDVINPRPGTLRYGALQKKPLWIIFARSMIIRLSKELMITSH
Sbjct: 121 KTTGGKKGRIYVVTDPSDNDVINPRPGTLRYGALQKKPLWIIFARSMIIRLSKELMITSH 180

Query: 181 KTIDARGANVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGD 240
           KTIDARGANVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGD
Sbjct: 181 KTIDARGANVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGD 240

Query: 241 GISIFGSTNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS 300
           GISIFGSTNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS
Sbjct: 241 GISIFGSTNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS 300

Query: 301 IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 360
           IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP
Sbjct: 301 IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 360

Query: 361 PNPAARQITNRNYATESVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSRFDMIKAKPG 420
           PNPAARQITNRNYATESVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSRFDMIKAKPG
Sbjct: 361 PNPAARQITNRNYATESVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSRFDMIKAKPG 420

Query: 421 TFVRRLTRFSGSLNCYVGRPC 441
           TFVRRLTRFSGSLNCYVGRPC
Sbjct: 421 TFVRRLTRFSGSLNCYVGRPC 441

BLAST of CsGy3G036830 vs. ExPASy TrEMBL
Match: A0A1S3B8R7 (Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103487022 PE=3 SV=1)

HSP 1 Score: 891 bits (2302), Expect = 0.0
Identity = 424/441 (96.15%), Postives = 438/441 (99.32%), Query Frame = 0

Query: 1   MMTGCKIKVVMVLVSMAVLMPTLRAGIAEFDDFLLQKSEEAKQASLEAFHPDPMNVTDHF 60
           M+ GCKIK++MVLVSMAVLMPTLRAGIAEFDD+LLQK+EEAKQASLEAFHPDPMNVTDHF
Sbjct: 1   MVAGCKIKLLMVLVSMAVLMPTLRAGIAEFDDYLLQKAEEAKQASLEAFHPDPMNVTDHF 60

Query: 61  NQHVHLALEGIEGSNSTRRSLSKYNGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGR 120
           NQHVHLALEGIEGSNSTRRSL+KY GPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGR
Sbjct: 61  NQHVHLALEGIEGSNSTRRSLAKYKGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGR 120

Query: 121 KTTGGKKGRIYVVTDPSDNDVINPRPGTLRYGALQKKPLWIIFARSMIIRLSKELMITSH 180
           KTTGGKKGRIYVVTDPSDNDV+NP+PGTLRYGALQK+PLWIIFARSMIIRLSKELM+TSH
Sbjct: 121 KTTGGKKGRIYVVTDPSDNDVVNPKPGTLRYGALQKRPLWIIFARSMIIRLSKELMVTSH 180

Query: 181 KTIDARGANVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGD 240
           KTIDARG+NVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGD
Sbjct: 181 KTIDARGSNVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGD 240

Query: 241 GISIFGSTNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS 300
           GISIFGS+NIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS
Sbjct: 241 GISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS 300

Query: 301 IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 360
           IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP
Sbjct: 301 IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 360

Query: 361 PNPAARQITNRNYATESVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSRFDMIKAKPG 420
           PNPAARQITNRNYA ESVWKTWTWRSEGDLMMNGAYF+QSGNPSKRRPYSRFDMIKAKPG
Sbjct: 361 PNPAARQITNRNYAPESVWKTWTWRSEGDLMMNGAYFIQSGNPSKRRPYSRFDMIKAKPG 420

Query: 421 TFVRRLTRFSGSLNCYVGRPC 441
           TFVRRLTRFSGSLNC+VGRPC
Sbjct: 421 TFVRRLTRFSGSLNCFVGRPC 441

BLAST of CsGy3G036830 vs. ExPASy TrEMBL
Match: A0A5A7UD78 (Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold135G001200 PE=3 SV=1)

HSP 1 Score: 850 bits (2197), Expect = 5.47e-311
Identity = 406/422 (96.21%), Postives = 419/422 (99.29%), Query Frame = 0

Query: 1   MMTGCKIKVVMVLVSMAVLMPTLRAGIAEFDDFLLQKSEEAKQASLEAFHPDPMNVTDHF 60
           M+ GCKIK++MVLVSMAVLMPTLRAGIAEFDD+LLQK+EEAKQASLEAFHPDPMNVTDHF
Sbjct: 1   MVAGCKIKLLMVLVSMAVLMPTLRAGIAEFDDYLLQKAEEAKQASLEAFHPDPMNVTDHF 60

Query: 61  NQHVHLALEGIEGSNSTRRSLSKYNGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGR 120
           NQHVHLALEGIEGSNSTRRSL+KY GPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGR
Sbjct: 61  NQHVHLALEGIEGSNSTRRSLAKYKGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGR 120

Query: 121 KTTGGKKGRIYVVTDPSDNDVINPRPGTLRYGALQKKPLWIIFARSMIIRLSKELMITSH 180
           KTTGGKKGRIYVVTDPSDNDV+NP+PGTLRYGALQK+PLWIIFARSMIIRLSKELM+TSH
Sbjct: 121 KTTGGKKGRIYVVTDPSDNDVVNPKPGTLRYGALQKRPLWIIFARSMIIRLSKELMVTSH 180

Query: 181 KTIDARGANVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGD 240
           KTIDARG+NVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGD
Sbjct: 181 KTIDARGSNVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGD 240

Query: 241 GISIFGSTNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS 300
           GISIFGS+NIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS
Sbjct: 241 GISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS 300

Query: 301 IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 360
           IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP
Sbjct: 301 IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 360

Query: 361 PNPAARQITNRNYATESVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSRFDMIKAKPG 420
           PNPAARQITNRNYA ESVWKTWTWRSEGDLMMNGAYF+QSGNPSKRRPYSRFDMIKAKPG
Sbjct: 361 PNPAARQITNRNYAPESVWKTWTWRSEGDLMMNGAYFIQSGNPSKRRPYSRFDMIKAKPG 420

Query: 421 TF 422
           TF
Sbjct: 421 TF 422

BLAST of CsGy3G036830 vs. ExPASy TrEMBL
Match: A0A5D3DPR0 (Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G008250 PE=3 SV=1)

HSP 1 Score: 828 bits (2138), Expect = 1.79e-302
Identity = 395/411 (96.11%), Postives = 408/411 (99.27%), Query Frame = 0

Query: 1   MMTGCKIKVVMVLVSMAVLMPTLRAGIAEFDDFLLQKSEEAKQASLEAFHPDPMNVTDHF 60
           M+ GCKIK++MVLVSMAVLMPTLRAGIAEFDD+LLQK+EEAKQASLEAFHPDPMNVTDHF
Sbjct: 1   MVAGCKIKLLMVLVSMAVLMPTLRAGIAEFDDYLLQKAEEAKQASLEAFHPDPMNVTDHF 60

Query: 61  NQHVHLALEGIEGSNSTRRSLSKYNGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGR 120
           NQHVHLALEGIEGSNSTRRSL+KY GPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGR
Sbjct: 61  NQHVHLALEGIEGSNSTRRSLAKYKGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGR 120

Query: 121 KTTGGKKGRIYVVTDPSDNDVINPRPGTLRYGALQKKPLWIIFARSMIIRLSKELMITSH 180
           KTTGGKKGRIYVVTDPSDNDV+NP+PGTLRYGALQK+PLWIIFARSMIIRLSKELM+TSH
Sbjct: 121 KTTGGKKGRIYVVTDPSDNDVVNPKPGTLRYGALQKRPLWIIFARSMIIRLSKELMVTSH 180

Query: 181 KTIDARGANVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGD 240
           KTIDARG+NVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGD
Sbjct: 181 KTIDARGSNVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGD 240

Query: 241 GISIFGSTNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS 300
           GISIFGS+NIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS
Sbjct: 241 GISIFGSSNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS 300

Query: 301 IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 360
           IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP
Sbjct: 301 IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 360

Query: 361 PNPAARQITNRNYATESVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSR 411
           PNPAARQITNRNYA ESVWKTWTWRSEGDLMMNGAYF+QSGNPSKRRPYSR
Sbjct: 361 PNPAARQITNRNYAPESVWKTWTWRSEGDLMMNGAYFIQSGNPSKRRPYSR 411

BLAST of CsGy3G036830 vs. ExPASy TrEMBL
Match: A0A6J1DFD2 (Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111019925 PE=3 SV=1)

HSP 1 Score: 811 bits (2095), Expect = 1.65e-295
Identity = 382/440 (86.82%), Postives = 413/440 (93.86%), Query Frame = 0

Query: 2   MTGCKIKVVMVLVSMAVLMPTLRAGIAEFDDFLLQKSEEAKQASLEAFHPDPMNVTDHFN 61
           M G +++ +M+LVS+AV++PTLRAGIAE+D++LLQK+EEAK ASLEAFH DPMNVTDHFN
Sbjct: 1   MAGFRVEFLMLLVSVAVILPTLRAGIAEYDEYLLQKAEEAKMASLEAFHSDPMNVTDHFN 60

Query: 62  QHVHLALEGIEGSNSTRRSLSKYNGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGRK 121
             VHLALEG   SNSTRRS+ KYNGPC+ATNPIDRCWRCDPNWA NRKKLA CVLGFGRK
Sbjct: 61  LQVHLALEG---SNSTRRSMKKYNGPCMATNPIDRCWRCDPNWANNRKKLATCVLGFGRK 120

Query: 122 TTGGKKGRIYVVTDPSDNDVINPRPGTLRYGALQKKPLWIIFARSMIIRLSKELMITSHK 181
           T GG KG+IYVVTDPSD DV+NP+PGTLRYGA+QKKPLWIIF RSMII LSKEL++ SHK
Sbjct: 121 TRGGVKGKIYVVTDPSDADVVNPKPGTLRYGAVQKKPLWIIFGRSMIITLSKELLVASHK 180

Query: 182 TIDARGANVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGDG 241
           TIDARGANVHIA+GAG+SIQFA+NVIIHGL IHH+I   GG+IRD+ NHVGLRT SDGDG
Sbjct: 181 TIDARGANVHIAFGAGISIQFAKNVIIHGLHIHHIIVATGGLIRDSTNHVGLRTNSDGDG 240

Query: 242 ISIFGSTNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDSI 301
           +SIFGSTNIWLDHLSMSNC DGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS+
Sbjct: 241 VSIFGSTNIWLDHLSMSNCADGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDSV 300

Query: 302 MQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPP 361
           MQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPP
Sbjct: 301 MQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPP 360

Query: 362 NPAARQITNRNYATESVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSRFDMIKAKPGT 421
           NPAA+QITNRNYATE+VWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSR DMIKAKPGT
Sbjct: 361 NPAAKQITNRNYATEAVWKTWTWRSEGDLMMNGAYFVQSGNPSKRRPYSRHDMIKAKPGT 420

Query: 422 FVRRLTRFSGSLNCYVGRPC 441
           FVRRLTRFSGSLNC+VGRPC
Sbjct: 421 FVRRLTRFSGSLNCFVGRPC 437

BLAST of CsGy3G036830 vs. TAIR 10
Match: AT3G01270.1 (Pectate lyase family protein )

HSP 1 Score: 578.2 bits (1489), Expect = 5.7e-165
Identity = 281/476 (59.03%), Postives = 349/476 (73.32%), Query Frame = 0

Query: 1   MMTGCKIKVVMVLVSMAVLMPTLRAGIA-EFDDFLLQKSEEAKQASLEAFHPDPMNVTDH 60
           M T    K+V V + +A L+PT+RA +A E D++ + K+ EA++ +L A+HPDP  + DH
Sbjct: 1   METARLFKLVCV-ICIASLIPTIRANVADETDEYWVNKANEARKHTLMAYHPDPYEIVDH 60

Query: 61  FNQHVHLALEGIEG---------------------SNSTRRSL------------SKYNG 120
           F++  +     +EG                     +NSTRRSL            SK  G
Sbjct: 61  FHERHYDNSTDVEGTEEEKAVASEEEDVIEMISSPTNSTRRSLTGRGKGKGKGKWSKLTG 120

Query: 121 PCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGRKTTGGKKGRIYVVTDPSDNDVINPRP 180
           PC A+NPID+CWRC P+WA+ RKKL  CV GFG +TTGGK+GRIYVVT P D+D++NPRP
Sbjct: 121 PCTASNPIDKCWRCQPDWARRRKKLVHCVRGFGYRTTGGKRGRIYVVTSPRDDDMVNPRP 180

Query: 181 GTLRYGALQKKPLWIIFARSMIIRLSKELMITSHKTIDARGANVHIAYGAGLSIQFARNV 240
           GTLR+  +QK+PLWI+F   M IRLS+ELMITS KTIDARGANVHIAYGAG+++Q+  N+
Sbjct: 181 GTLRHAVIQKEPLWIVFKHDMSIRLSQELMITSDKTIDARGANVHIAYGAGITMQYVHNI 240

Query: 241 IIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGDGISIFGSTNIWLDHLSMSNCQDGLID 300
           IIHGL +HH++ + GG+IRD+ NH G R  +DGDGISIFG+TNIWLDH+SMS CQDGLID
Sbjct: 241 IIHGLHVHHIVKSSGGLIRDSINHFGHRGEADGDGISIFGATNIWLDHISMSKCQDGLID 300

Query: 301 AIQGSTAITISNSHFTHHNDVMLFGASDSYQGDSIMQVTVAFNHFGKGLVQRMPRCRWGF 360
           AI GSTAITISNSHFTHHNDVML GA ++   D  MQVTVA+NHFGKGLVQRMPR RWGF
Sbjct: 301 AIMGSTAITISNSHFTHHNDVMLLGAQNNNMDDKKMQVTVAYNHFGKGLVQRMPRVRWGF 360

Query: 361 FHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNPA-ARQITNRNYATESVWKTWTWR 420
            HVVNNDYTHW +YAIGGSQ PTI+S GNRFIAPP+    R++T R+YA+ES WK W WR
Sbjct: 361 VHVVNNDYTHWELYAIGGSQGPTILSHGNRFIAPPHKQHYREVTKRDYASESEWKNWNWR 420

Query: 421 SEGDLMMNGAYFVQSGNPSKRRPYSRFDMIKAKPGTFVRRLTRFSGSLNCYVGRPC 442
           SE D+ MN AYF QSGNP  +  +SR  MIK K G  V +LT+++G+L+C VG+ C
Sbjct: 421 SEKDVFMNNAYFRQSGNPHFKCSHSRQQMIKPKNGMAVSKLTKYAGALDCRVGKAC 475

BLAST of CsGy3G036830 vs. TAIR 10
Match: AT5G15110.1 (Pectate lyase family protein )

HSP 1 Score: 573.5 bits (1477), Expect = 1.4e-163
Identity = 272/468 (58.12%), Postives = 348/468 (74.36%), Query Frame = 0

Query: 6   KIKVVMVLVSMAVLMPTLRAGIAEFDDFLLQKSEEAKQASLEAFHPDPMNVTDHFNQHVH 65
           ++  +M    +AVL+PT+R  I+E D++  Q+++EA++ +L+A+H DP  + DHF++  +
Sbjct: 5   RLSKLMFTFCIAVLIPTIRGNISELDEYWSQRADEAREFTLQAYHSDPYEIVDHFHERHY 64

Query: 66  -------------------LALEGIE----GSNSTRRSL--------SKYNGPCLATNPI 125
                                 E IE     +NSTRRSL        SK  GPC A+NPI
Sbjct: 65  DNSTDVTTPEEDGDAKPEEEEKEFIEMLGSSTNSTRRSLRGKGKGKWSKLKGPCTASNPI 124

Query: 126 DRCWRCDPNWAKNRKKLAKCVLGFGRKTTGGKKGRIYVVTDPSDNDVINPRPGTLRYGAL 185
           D+CWRC  +WAK RKKL +CV GFG +TTGGK+GRIYVVT   D D++NP+PGTLR+  +
Sbjct: 125 DKCWRCRSDWAKRRKKLTRCVRGFGHRTTGGKRGRIYVVTSNLDEDMVNPKPGTLRHAVI 184

Query: 186 QKKPLWIIFARSMIIRLSKELMITSHKTIDARGANVHIAYGAGLSIQFARNVIIHGLRIH 245
           QK+PLWIIF   M IRL++EL+I SHKTIDARGANVH+A+GAG+++QF +NV+IHGL IH
Sbjct: 185 QKEPLWIIFKNDMSIRLNQELLINSHKTIDARGANVHVAHGAGITMQFVKNVVIHGLHIH 244

Query: 246 HVISTGGGMIRDTANHVGLRTVSDGDGISIFGSTNIWLDHLSMSNCQDGLIDAIQGSTAI 305
           H+  + GGMIRD+ +H G+RT +DGDG+SI+GS+NIWLDH+SMS CQDGLIDAI GST I
Sbjct: 245 HISESSGGMIRDSVDHFGMRTRADGDGLSIYGSSNIWLDHISMSKCQDGLIDAIVGSTGI 304

Query: 306 TISNSHFTHHNDVMLFGASDSYQGDSIMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDY 365
           TISNSHFTHHNDVML GA ++ + D  MQVTVA+NHFGKGLVQRMPR RWGF HVVNNDY
Sbjct: 305 TISNSHFTHHNDVMLLGAQNTNEADKHMQVTVAYNHFGKGLVQRMPRIRWGFVHVVNNDY 364

Query: 366 THWLMYAIGGSQHPTIISQGNRFIAPPN-PAARQITNRNYATESVWKTWTWRSEGDLMMN 425
           THW +YAIGGSQ PTI+S GNRFIAPP+ P  R++T R+YA+E  WK W WRS+ D+ MN
Sbjct: 365 THWELYAIGGSQGPTILSHGNRFIAPPHKPHYREVTKRDYASEDEWKHWNWRSDKDVFMN 424

Query: 426 GAYFVQSGNPSKRRPYSRFDMIKAKPGTFVRRLTRFSGSLNCYVGRPC 442
           GAYF QSGNP  +  ++R  MIK K G  V +LT+++G+L+C VGR C
Sbjct: 425 GAYFRQSGNPQYKCAHTRQQMIKPKNGLAVSKLTKYAGALDCRVGRRC 472

BLAST of CsGy3G036830 vs. TAIR 10
Match: AT1G14420.1 (Pectate lyase family protein )

HSP 1 Score: 556.2 bits (1432), Expect = 2.3e-158
Identity = 262/440 (59.55%), Postives = 323/440 (73.41%), Query Frame = 0

Query: 16  MAVLMPTLRAGIAEFDDFLLQKSEEAKQASLEAFHPDPMNVTDHFNQHVHLALEGIEGSN 75
           +A++ P +R  +A FD +  Q+  +A + ++ ++ P P+NVT+HFN HV++A++  E  N
Sbjct: 20  LAIVAPQVRGNVAVFDSYWTQRQSDALKQTIGSYDPHPLNVTNHFNYHVNIAVDASESRN 79

Query: 76  STRRSLS---------KYNGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGRKTTGGK 135
            TRR L+         K +G CLA NPID CWRCD NWA NRKKLA CVLGFGR+TTGGK
Sbjct: 80  DTRRELTQVRSGRKTHKSSGKCLAYNPIDNCWRCDRNWANNRKKLADCVLGFGRRTTGGK 139

Query: 136 KGRIYVVTDPSDNDVINPRPGTLRYGALQKKPLWIIFARSMIIRLSKELMITSHKTIDAR 195
            G IYVV D SDND+INP+PGTLR+   +  PLWIIFARSMII+L +ELMITS KTID R
Sbjct: 140 DGPIYVVKDASDNDLINPKPGTLRHAVTRDGPLWIIFARSMIIKLQQELMITSDKTIDGR 199

Query: 196 GANVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGDGISIFG 255
           GA V+I  GAGL++QF  NVIIH + + H++   GG+IRD+  H+GLRT SDGDGIS+FG
Sbjct: 200 GARVYIMEGAGLTLQFVNNVIIHNIYVKHIVPGNGGLIRDSEAHIGLRTKSDGDGISLFG 259

Query: 256 STNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDSIMQVTV 315
           +TNIW+DH+SM+ C DG+IDAI GSTA+TISNSHFT H +VMLFGA D +  D  MQ+TV
Sbjct: 260 ATNIWIDHVSMTRCADGMIDAIDGSTAVTISNSHFTDHQEVMLFGARDEHVIDKKMQITV 319

Query: 316 AFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAPPNPAAR 375
           AFNHFGK L QRMPRCR+G  HVVNNDYTHW MYAIGG+ +PTIISQGNRFIAPPN  A+
Sbjct: 320 AFNHFGKRLEQRMPRCRYGTIHVVNNDYTHWEMYAIGGNMNPTIISQGNRFIAPPNEEAK 379

Query: 376 QITNRNYATESVWKTWTWRSEGDLMMNGAYFVQSG-----NPSKRRPYSRFDMIKAKPGT 435
           QIT R Y     WK+W W+SEGD  +NGAYFVQSG     +   + P      I+ KPGT
Sbjct: 380 QITKREYTPYGEWKSWNWQSEGDYFLNGAYFVQSGKANAWSSKPKTPLPNKFTIRPKPGT 439

Query: 436 FVRRLTRFSGSLNCYVGRPC 442
            VR+LT  +G L C +G  C
Sbjct: 440 MVRKLTMDAGVLGCKLGEAC 459

BLAST of CsGy3G036830 vs. TAIR 10
Match: AT2G02720.1 (Pectate lyase family protein )

HSP 1 Score: 527.3 bits (1357), Expect = 1.2e-149
Identity = 255/446 (57.17%), Postives = 326/446 (73.09%), Query Frame = 0

Query: 10  VMVLVSMAVLMPTLRAGIAEFDDFLLQKSEEAKQASLEAFHPDPMNVTDHFNQHVHLALE 69
           V V +S+ V++P+++A IAE+D++  Q+   A + +LE++ P+P NVTDHFN H  LA+E
Sbjct: 10  VFVALSLTVVVPSVQAHIAEYDEYWTQRQTNALRETLESYDPNPENVTDHFNYHAALAME 69

Query: 70  GIEGSNSTRRSL---------SKYNGPCLATNPIDRCWRCDPNWAKNRKKLAKCVLGFGR 129
                N TRR L         ++  G   + N ID+CWR D NW KNRKKLA CVLGFGR
Sbjct: 70  TTGIVNETRRDLRQVGRGKKTTRRGGRFESLNAIDKCWRGDKNWDKNRKKLADCVLGFGR 129

Query: 130 KTTGGKKGRIYVVTDPSDNDVINPRPGTLRYGALQKKPLWIIFARSMIIRLSKELMITSH 189
           KTTGGK G IYVVTDPSDND++ P+PGT+R+   + +PLWIIFARSMII+L +EL+IT+ 
Sbjct: 130 KTTGGKNGPIYVVTDPSDNDLLKPKPGTIRHAVTRDRPLWIIFARSMIIKLQQELIITND 189

Query: 190 KTIDARGANVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGGMIRDTANHVGLRTVSDGD 249
           KTID RGA ++I  GAGL++QF RNVIIH + I  +    GG+I D+  H GLRTVSDGD
Sbjct: 190 KTIDGRGAKIYITGGAGLTLQFVRNVIIHNIHIKQIKRGAGGLIIDSEQHFGLRTVSDGD 249

Query: 250 GISIFGSTNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFTHHNDVMLFGASDSYQGDS 309
           GI+IFG+TN+W+DH+SM++C DG+IDAI GSTAITISNSHFT H++VMLFG ++    D 
Sbjct: 250 GINIFGATNVWIDHVSMTDCSDGMIDAIMGSTAITISNSHFTDHDEVMLFGGTNKDVIDK 309

Query: 310 IMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSQHPTIISQGNRFIAP 369
            MQ+TVAFNHFGK L QRMPR R+G  HVVNNDYTHW MYAIGG+ +PTIISQGNRFIAP
Sbjct: 310 KMQITVAFNHFGKRLKQRMPRVRFGLVHVVNNDYTHWEMYAIGGNMNPTIISQGNRFIAP 369

Query: 370 PNPAARQITNRNYATESVWKTWTWRSEGDLMMNGAYFVQSG-----NPSKRRPYSRFDMI 429
           P   ++Q+T R Y     WK+W W+SE D  +NGAYFVQSG     + + + P  R   I
Sbjct: 370 PIEDSKQVTKREYTPYPEWKSWNWQSEKDYFLNGAYFVQSGKANAWSATPKNPIPRKFAI 429

Query: 430 KAKPGTFVRRLTRFSGSLNCYVGRPC 442
           + +PGT VRRLT+ +G+L C  G+ C
Sbjct: 430 RPQPGTKVRRLTKDAGTLGCKPGKSC 455

BLAST of CsGy3G036830 vs. TAIR 10
Match: AT4G13710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 479.2 bits (1232), Expect = 3.6e-135
Identity = 225/399 (56.39%), Postives = 285/399 (71.43%), Query Frame = 0

Query: 43  QASLEAFHPDPMNVTDHFNQHVHLALEGIEGSNSTRRSLSKYNGPCLATNPIDRCWRCDP 102
           Q    A   DP  V D   + V ++    E + + RR L  ++  C   NPID CWRCD 
Sbjct: 79  QQQQHAVADDPDMVADEVAKLVQMS----EQNRTARRKLGFFS--CGTGNPIDDCWRCDR 138

Query: 103 NWAKNRKKLAKCVLGFGRKTTGGKKGRIYVVTDPSDNDVINPRPGTLRYGALQKKPLWII 162
           NW KNRK+LA C +GFGR   GG+ GR Y+VTDP+D DV+NP+PGTLR+  +Q++PLWI+
Sbjct: 139 NWHKNRKRLADCGIGFGRNAIGGRDGRFYIVTDPTDEDVVNPKPGTLRHAVIQEEPLWIV 198

Query: 163 FARSMIIRLSKELMITSHKTIDARGANVHIAYGAGLSIQFARNVIIHGLRIHHVISTGGG 222
           F R M+I L +EL++ S KTIDARG+NVHIA GA ++IQF  NVIIHGL IH    TG  
Sbjct: 199 FKRDMVIELKQELIMNSFKTIDARGSNVHIANGACITIQFITNVIIHGLHIHDCKPTGNA 258

Query: 223 MIRDTANHVGLRTVSDGDGISIFGSTNIWLDHLSMSNCQDGLIDAIQGSTAITISNSHFT 282
           M+R + +H G RT++DGD +SIFGS++IW+DH S+S+C DGL+DA+ GSTAIT+SN+HFT
Sbjct: 259 MVRSSPSHFGWRTMADGDAVSIFGSSHIWIDHNSLSHCADGLVDAVMGSTAITVSNNHFT 318

Query: 283 HHNDVMLFGASDSYQGDSIMQVTVAFNHFGKGLVQRMPRCRWGFFHVVNNDYTHWLMYAI 342
           HHN+VML G SDSY  D +MQVT+A+NHFG+GLVQRMPRCR G+FHVVNNDYTHW MYAI
Sbjct: 319 HHNEVMLLGHSDSYTKDKLMQVTIAYNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAI 378

Query: 343 GGSQHPTIISQGNRFIAPPNPAARQITNRNYATESVWKTWTWRSEGDLMMNGAYFVQSGN 402
           GGS  PTI SQGNR+ AP +  A+++T R     S WK W WRSEGDL++NGA+F  SG 
Sbjct: 379 GGSAEPTINSQGNRYAAPMDRFAKEVTKRVETDASEWKKWNWRSEGDLLLNGAFFRPSG- 438

Query: 403 PSKRRPYSRFDMIKAKPGTFVRRLTRFSGSLNCYVGRPC 442
                 Y R   + AKP + V  +T  +G+L C  GRPC
Sbjct: 439 AGASASYGRASSLAAKPSSMVDTITSTAGALGCRKGRPC 470

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SRH48.1e-16459.03Probable pectate lyase 7 OS=Arabidopsis thaliana OX=3702 GN=At3g01270 PE=2 SV=1[more]
Q9LFP51.5e-16258.33Probable pectate lyase 19 OS=Arabidopsis thaliana OX=3702 GN=At5g15110 PE=2 SV=1[more]
P409733.5e-15963.64Pectate lyase OS=Lilium longiflorum OX=4690 PE=2 SV=1[more]
Q9M9S23.3e-15759.55Probable pectate lyase 3 OS=Arabidopsis thaliana OX=3702 GN=AT59 PE=2 SV=1[more]
P157226.9e-15558.44Probable pectate lyase P59 OS=Solanum lycopersicum OX=4081 GN=LAT59 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_004147462.20.0100.00probable pectate lyase 7 [Cucumis sativus] >KGN59593.1 hypothetical protein Csa_... [more]
XP_008443432.10.096.15PREDICTED: probable pectate lyase 19 [Cucumis melo][more]
KAA0053742.11.13e-31096.21putative pectate lyase 19 [Cucumis melo var. makuwa][more]
XP_038905811.11.61e-31091.99pectate lyase-like [Benincasa hispida][more]
TYK25661.13.69e-30296.11putative pectate lyase 19 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
A0A0A0LCU30.0100.00Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_3G827350 PE=3 SV=1[more]
A0A1S3B8R70.096.15Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103487022 PE=3 SV=1[more]
A0A5A7UD785.47e-31196.21Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold135G001200... [more]
A0A5D3DPR01.79e-30296.11Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G008250... [more]
A0A6J1DFD21.65e-29586.82Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111019925 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G01270.15.7e-16559.03Pectate lyase family protein [more]
AT5G15110.11.4e-16358.12Pectate lyase family protein [more]
AT1G14420.12.3e-15859.55Pectate lyase family protein [more]
AT2G02720.11.2e-14957.17Pectate lyase family protein [more]
AT4G13710.13.6e-13556.39Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018082AmbAllergenPRINTSPR00807AMBALLERGENcoord: 412..435
score: 42.66
coord: 318..337
score: 78.1
coord: 382..406
score: 53.71
coord: 174..190
score: 65.27
coord: 138..163
score: 63.37
coord: 238..259
score: 61.47
coord: 340..359
score: 79.52
coord: 114..131
score: 61.64
IPR002022Pectate lyaseSMARTSM00656amb_allcoord: 166..363
e-value: 1.6E-98
score: 343.3
IPR002022Pectate lyasePFAMPF00544Pectate_lyase_4coord: 177..357
e-value: 8.3E-19
score: 68.0
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 91..434
e-value: 1.6E-113
score: 381.2
IPR007524Pectate lyase, N-terminalPFAMPF04431Pec_lyase_Ncoord: 27..81
e-value: 1.6E-18
score: 66.7
NoneNo IPR availablePANTHERPTHR31683:SF146PECTATE LYASEcoord: 7..441
IPR045032Pectin lyase familyPANTHERPTHR31683PECTATE LYASE 18-RELATEDcoord: 7..441
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 92..431

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy3G036830.2CsGy3G036830.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045490 pectin catabolic process
molecular_function GO:0046872 metal ion binding
molecular_function GO:0030570 pectate lyase activity