CsGy3G036460 (gene) Cucumber (Gy14) v2.1

Overview


Name	CsGy3G036460
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description	Ribonuclease T(2)
Location	Gy14Chr3: 34586234 .. 34587337 (-)
RNA-Seq Expression	CsGy3G036460
Synteny	CsGy3G036460

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAAAAACAAAGAGTGTCGATGTTGTGTTCTTCGTGTTTGTCTTAACCATTTTGTTTCCTATCGTTAAATCGCAAACTTTCGATGATTTTTGGTTTGTTCAGCAATGGCCACCAGCTATTTGTACTTTACAATCAGGACGATGTGTAGGACGAGGGACTCGATCCTTCACAATTCATGGTTTGTGGCCTCAAAAGGGAGGACGTTCTGTGACTAATTGTACTGGCAATCAATTTGATTTCACTAAGGTAAAATTCAAACATCCAAAATATATAATCTATCTTTTTTTAAGTTTAATTTTCAGTTGGTTAATGTATCATATATTATTATTATTTCTTGTTTAAGAGTTAATGGATAGTTTATGGTAGTCATTCTTTTTTGTTTTTGTATTCGTTCTTATATTTTAAAATGATATAGTTGTTTCTTATTTATCAAACTTCTAAAACATCTCTTCATCTCTTATCTAAAAACATTGACAACTAAGTTTGATTTTCTGTTTAGAAAATAAAAGTGTTAGCAAACATACTACAGTGCTTCAATTTAAAAACCAAATGAAATATTATAAGAAATTTGAAAGGAATTATACATGATTATTAAGTATTATGTTTTGAAAATTTATTAAATAATCGGTTTTGATAGTTAAAAAAGAAAAAGGGTTTGCAATTCTAAAGATTGAATAATGTGTGGGTGTGTTTCAGATTGCACATTTAGAAAACGATCTAAACGTAGTTTGGCCGAATGTGGTGACGGGAAACAACAAATTCTTTTGGGGTCATGAATGGAACAAACATGGGATTTGCTCAGAGAGCAAGTTTGACGAAGCGAAGTACTTCCAAACAGCGATAAACATGAGGCACGGTATCGACCTTCTTAGTGTGCTGAGAACTGGTGGAGTAGGACCAAATGGAGCCTCCAAGGCAAAGCAAAGAGTCGAAACCGCGATTTCATCCCATTTTGGAAAAGATCCAATTCTTCGATGTAAAAAAGCATCAAACGGCCAAGTCTTATTGACGGAGATTGTGATGTGCTTCGATGACGATGGTGTCACCCTTATAAACTGTAACAAAGCAAGATCCAATTGTGCTGGGAGCTTTATTTTTTAA

mRNA sequence

ATGGAAAAAACAAAGAGTGTCGATGTTGTGTTCTTCGTGTTTGTCTTAACCATTTTGTTTCCTATCGTTAAATCGCAAACTTTCGATGATTTTTGGTTTGTTCAGCAATGGCCACCAGCTATTTGTACTTTACAATCAGGACGATGTGTAGGACGAGGGACTCGATCCTTCACAATTCATGGTTTGTGGCCTCAAAAGGGAGGACGTTCTGTGACTAATTGTACTGGCAATCAATTTGATTTCACTAAGATTGCACATTTAGAAAACGATCTAAACGTAGTTTGGCCGAATGTGGTGACGGGAAACAACAAATTCTTTTGGGGTCATGAATGGAACAAACATGGGATTTGCTCAGAGAGCAAGTTTGACGAAGCGAAGTACTTCCAAACAGCGATAAACATGAGGCACGGTATCGACCTTCTTAGTGTGCTGAGAACTGGTGGAGTAGGACCAAATGGAGCCTCCAAGGCAAAGCAAAGAGTCGAAACCGCGATTTCATCCCATTTTGGAAAAGATCCAATTCTTCGATGTAAAAAAGCATCAAACGGCCAAGTCTTATTGACGGAGATTGTGATGTGCTTCGATGACGATGGTGTCACCCTTATAAACTGTAACAAAGCAAGATCCAATTGTGCTGGGAGCTTTATTTTTTAA

Coding sequence (CDS)

ATGGAAAAAACAAAGAGTGTCGATGTTGTGTTCTTCGTGTTTGTCTTAACCATTTTGTTTCCTATCGTTAAATCGCAAACTTTCGATGATTTTTGGTTTGTTCAGCAATGGCCACCAGCTATTTGTACTTTACAATCAGGACGATGTGTAGGACGAGGGACTCGATCCTTCACAATTCATGGTTTGTGGCCTCAAAAGGGAGGACGTTCTGTGACTAATTGTACTGGCAATCAATTTGATTTCACTAAGATTGCACATTTAGAAAACGATCTAAACGTAGTTTGGCCGAATGTGGTGACGGGAAACAACAAATTCTTTTGGGGTCATGAATGGAACAAACATGGGATTTGCTCAGAGAGCAAGTTTGACGAAGCGAAGTACTTCCAAACAGCGATAAACATGAGGCACGGTATCGACCTTCTTAGTGTGCTGAGAACTGGTGGAGTAGGACCAAATGGAGCCTCCAAGGCAAAGCAAAGAGTCGAAACCGCGATTTCATCCCATTTTGGAAAAGATCCAATTCTTCGATGTAAAAAAGCATCAAACGGCCAAGTCTTATTGACGGAGATTGTGATGTGCTTCGATGACGATGGTGTCACCCTTATAAACTGTAACAAAGCAAGATCCAATTGTGCTGGGAGCTTTATTTTTTAA

Protein sequence

MEKTKSVDVVFFVFVLTILFPIVKSQTFDDFWFVQQWPPAICTLQSGRCVGRGTRSFTIHGLWPQKGGRSVTNCTGNQFDFTKIAHLENDLNVVWPNVVTGNNKFFWGHEWNKHGICSESKFDEAKYFQTAINMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKASNGQVLLTEIVMCFDDDGVTLINCNKARSNCAGSFIF*

Homology

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BLAST of CsGy3G036460 vs. ExPASy Swiss-Prot
Match: P23540 (Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 1.3e-58
Identity = 101/193 (52.33%), Postives = 133/193 (68.91%), Query Frame = 0


Query: 28  FDDFWFVQQWPPAICTLQ-SGRCVGRGTRSFTIHGLWPQKGGRSVTNC-TGNQFDFTKIA 87
           FD FWFVQQWPPA+C+ Q SG C G G R+FTIHGLWPQ  G S+TNC  G+ FD TKI+
Sbjct: 1   FDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFTIHGLWPQGSGTSLTNCPQGSPFDITKIS 60

Query: 88  HLENDLNVVWPNVVTGNNKFFWGHEWNKHGICSESKFDEAKYFQTAINMRHGIDLLSVLR 147
           HL++ LN +WPNV+  NN+ FW HEW KHG CSES F++A YF+ A++MR+  D++  LR
Sbjct: 61  HLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYDIIGALR 120

Query: 148 TGGVGPNGASKAKQRVETAISSHFGKDPILRCKKASNGQV-LLTEIVMCFDDDGVTLINC 207
               GPNG +K++Q ++  + + FGK P LRC+     +V  L ++V CF  DG TLI+C
Sbjct: 121 PHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVQVVACFAQDGSTLIDC 180

Query: 208 NKARSNCAGSFIF 218
              R  C  +FIF
Sbjct: 181 --TRDTCGANFIF 191

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BLAST of CsGy3G036460 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 6.6e-34
Identity = 73/201 (36.32%), Postives = 107/201 (53.23%), Query Frame = 0


Query: 16  LTILFPIV-----KSQTFDDFWFVQQWPPAICTLQSGRCV---GRGTRSFTIHGLWPQ-K 75
           L ++ P V      S+ FD F+FVQQWP + C  Q   C    G+    F IHGLWP  K
Sbjct: 13  LLVILPSVFSASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGIHGLWPNYK 72

Query: 76  GGRSVTNCTGNQ-FDFTKIAHLENDLNVVWPNVV--TGNNKFFWGHEWNKHGICSESKFD 135
            G   +NC  ++ FD + I+ L   +   WP +   +G+ + FW HEW KHG CSES  D
Sbjct: 73  DGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSESVID 132

Query: 136 EAKYFQTAINMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKASNG 195
           + +YFQTA+N++   +LL  L   G+ P+G S + + +  +I    G  P + C +  +G
Sbjct: 133 QHEYFQTALNLKQKTNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVECNRDGSG 192

Query: 196 QVLLTEIVMCFDDDGVTLINC 205
              L ++ +C D  G  LI C
Sbjct: 193 NSQLYQVYLCVDRSGSGLIEC 213

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BLAST of CsGy3G036460 vs. ExPASy Swiss-Prot
Match: P80022 (Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2)

HSP 1 Score: 132.1 bits (331), Expect = 7.6e-30
Identity = 68/213 (31.92%), Postives = 112/213 (52.58%), Query Frame = 0


Query: 1   MEKTKSVDVVFFVFVLTILFPIVK-SQTFDDFWFVQQWPPAICTLQSGRC---VGRGTRS 60
           M    +  +   + ++T    ++  ++ FD F+FVQQWP + C  +   C    G+    
Sbjct: 1   MASNSAFSLFLILLIITQCLSVLNAAKDFDFFYFVQQWPGSYCDTKQSCCYPTTGKPAAD 60

Query: 61  FTIHGLWPQKG-GRSVTNCTGNQ-FDFTKIAHLENDLNVVWPNVV--TGNNKFFWGHEWN 120
           F IHGLWP    G   +NC  N  +D ++I+ L + +   WP +   +G+   FW HEW 
Sbjct: 61  FGIHGLWPNNNDGTYPSNCDPNSPYDQSQISDLISSMQQNWPTLACPSGSGSTFWSHEWE 120

Query: 121 KHGICSESKF-DEAKYFQTAINMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGK 180
           KHG C+ES   ++  YF+ A+++++ IDLLS+L+   + P+G S     +  AI S  G 
Sbjct: 121 KHGTCAESVLTNQHAYFKKALDLKNQIDLLSILQGADIHPDGESYDLVNIRNAIKSAIGY 180

Query: 181 DPILRCKKASNGQVLLTEIVMCFDDDGVTLINC 205
            P ++C    +G   L ++ +C D  G +LI C
Sbjct: 181 TPWIQCNVDQSGNSQLYQVYICVDGSGSSLIEC 213

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BLAST of CsGy3G036460 vs. ExPASy Swiss-Prot
Match: P42815 (Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 4.9e-29
Identity = 71/203 (34.98%), Postives = 102/203 (50.25%), Query Frame = 0


Query: 11  FFVFVLTILFPIVKS--QTFDDFWFVQQWPPAICTLQSGRC---VGRGTRSFTIHGLWPQ 70
           FF+F+L +    V+S  Q FD F+FV QWP A C  +   C    G+    F IHGLWP 
Sbjct: 3   FFIFILALQQLYVQSFAQDFDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLWPN 62

Query: 71  -KGGRSVTNCT-GNQFDFTKIAHLENDLNVVWPNVVTGNN--KFFWGHEWNKHGICSESK 130
            K G    NC   ++FD  +++ L +DL   WP +   +N    FW HEW KHG C+ES+
Sbjct: 63  YKTGGWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESE 122

Query: 131 FDEAKYFQTAINMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKAS 190
            D+  YF+  + ++   +LL  L   G+ P+      + +E  I    G  P + C K S
Sbjct: 123 LDQHDYFEAGLKLKQKANLLHALTNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECNKDS 182

Query: 191 NGQVLLTEIVMCFDDDGVTLINC 205
           +    L +I +C D      INC
Sbjct: 183 SHNSQLYQIYLCVDTSASKFINC 205

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BLAST of CsGy3G036460 vs. ExPASy Swiss-Prot
Match: P80196 (Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2)

HSP 1 Score: 119.4 bits (298), Expect = 5.1e-26
Identity = 68/212 (32.08%), Postives = 105/212 (49.53%), Query Frame = 0


Query: 1   MEKTKSVDVVFFVFVLTILFPIVKSQTFDDFWFVQQWPPAICTLQSGRC---VGRGTRSF 60
           M K++   ++  + V  +L   V SQ FD F+FVQQWP + C  +   C    G+    F
Sbjct: 1   MMKSQKKLLIKIIVVQCLLVLCVTSQDFDFFYFVQQWPASYCDTRRSCCYPTTGKPDEDF 60

Query: 61  TIHGLWPQ-KGGRSVTNC-TGNQFDFTKIAHLENDLNVVWPNVV--TGNNKFFWGHEWNK 120
           +IHGLWP  K G+   NC   +  D ++ + L + +   WP++   + +   FW HEW K
Sbjct: 61  SIHGLWPNYKDGKWPQNCDRESSLDESEFSDLISTMEKNWPSLACPSSDGLKFWSHEWLK 120

Query: 121 HGICSESKFDEAKYFQTAINMRHGIDLLSVLRTGGVGP-NGASKAKQRVETAISSHFGKD 180
           HG C  S  ++  YFQTA++ +   +LL  L   G+ P NG     + ++ AI    G  
Sbjct: 121 HGTC--SALNQHAYFQTALDFKTKSNLLQNLNNAGIKPRNGDYYGVESIKKAIEKGVGHT 180

Query: 181 PILRCKKASNGQVLLTEIVMCFDDDGVTLINC 205
           P + C   S G   L ++ +C D      I+C
Sbjct: 181 PFIECNVDSQGNHQLYQVYLCVDSSASKFIDC 210

[Close]

BLAST of CsGy3G036460 vs. NCBI nr
Match: XP_004147499.1 (ribonuclease MC [Cucumis sativus] >KGN59560.1 hypothetical protein Csa_002553 [Cucumis sativus])

HSP 1 Score: 458 bits (1179), Expect = 4.67e-163
Identity = 216/217 (99.54%), Postives = 217/217 (100.00%), Query Frame = 0


Query: 1   MEKTKSVDVVFFVFVLTILFPIVKSQTFDDFWFVQQWPPAICTLQSGRCVGRGTRSFTIH 60
           MEKTKSVDVVFFVFVLTILFPIVKSQTFDDFWFVQQWPPA+CTLQSGRCVGRGTRSFTIH
Sbjct: 1   MEKTKSVDVVFFVFVLTILFPIVKSQTFDDFWFVQQWPPAVCTLQSGRCVGRGTRSFTIH 60

Query: 61  GLWPQKGGRSVTNCTGNQFDFTKIAHLENDLNVVWPNVVTGNNKFFWGHEWNKHGICSES 120
           GLWPQKGGRSVTNCTGNQFDFTKIAHLENDLNVVWPNVVTGNNKFFWGHEWNKHGICSES
Sbjct: 61  GLWPQKGGRSVTNCTGNQFDFTKIAHLENDLNVVWPNVVTGNNKFFWGHEWNKHGICSES 120

Query: 121 KFDEAKYFQTAINMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKA 180
           KFDEAKYFQTAINMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKA
Sbjct: 121 KFDEAKYFQTAINMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKA 180

Query: 181 SNGQVLLTEIVMCFDDDGVTLINCNKARSNCAGSFIF 217
           SNGQVLLTEIVMCFDDDGVTLINCNKARSNCAGSFIF
Sbjct: 181 SNGQVLLTEIVMCFDDDGVTLINCNKARSNCAGSFIF 217

[Close]

BLAST of CsGy3G036460 vs. NCBI nr
Match: XP_008443402.1 (PREDICTED: ribonuclease MC-like [Cucumis melo])

HSP 1 Score: 427 bits (1098), Expect = 1.01e-150
Identity = 203/217 (93.55%), Postives = 209/217 (96.31%), Query Frame = 0


Query: 1   MEKTKSVDVVFFVFVLTILFPIVKSQTFDDFWFVQQWPPAICTLQSGRCVGRGTRSFTIH 60
           MEKTK  DVVFFVFVLTILFPIVKSQTFD+FWFVQQWPPAICTLQSGRCVGRGTRSFTIH
Sbjct: 1   MEKTKR-DVVFFVFVLTILFPIVKSQTFDNFWFVQQWPPAICTLQSGRCVGRGTRSFTIH 60

Query: 61  GLWPQKGGRSVTNCTGNQFDFTKIAHLENDLNVVWPNVVTGNNKFFWGHEWNKHGICSES 120
           GLWPQKGGRSVTNC+GN FDFTKIAHLE+DLNVVWPNVVTGNNKFFWGHEWNKHGICSES
Sbjct: 61  GLWPQKGGRSVTNCSGNSFDFTKIAHLESDLNVVWPNVVTGNNKFFWGHEWNKHGICSES 120

Query: 121 KFDEAKYFQTAINMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKA 180
           KFD+AKYFQTAINMRH IDLLSVLRTGG+ PNGASKAKQRVETAISSHF KDPILRCK A
Sbjct: 121 KFDQAKYFQTAINMRHSIDLLSVLRTGGIVPNGASKAKQRVETAISSHFAKDPILRCKTA 180

Query: 181 SNGQVLLTEIVMCFDDDGVTLINCNKARSNCAGSFIF 217
           SNGQVLLTEIV+CFDDDGVTLINCNKARSNCA SFIF
Sbjct: 181 SNGQVLLTEIVICFDDDGVTLINCNKARSNCARSFIF 216

[Close]

BLAST of CsGy3G036460 vs. NCBI nr
Match: XP_038905534.1 (ribonuclease MC-like [Benincasa hispida])

HSP 1 Score: 406 bits (1043), Expect = 2.46e-142
Identity = 189/217 (87.10%), Postives = 200/217 (92.17%), Query Frame = 0


Query: 1   MEKTKSVDVVFFVFVLTILFPIVKSQTFDDFWFVQQWPPAICTLQSGRCVGRGTRSFTIH 60
           M  TK  ++V  VF LTILFPIV+SQTFDDFWFVQQWPPA+C LQSGRCVGRGTRSFTIH
Sbjct: 1   MATTKR-NIVLLVFGLTILFPIVQSQTFDDFWFVQQWPPAVCALQSGRCVGRGTRSFTIH 60

Query: 61  GLWPQKGGRSVTNCTGNQFDFTKIAHLENDLNVVWPNVVTGNNKFFWGHEWNKHGICSES 120
           GLWPQKGG SVTNC GN FDFTKIAHLENDLN+VWPNV+TGNNKFFWGHEWNKHGICSES
Sbjct: 61  GLWPQKGGSSVTNCAGNSFDFTKIAHLENDLNIVWPNVITGNNKFFWGHEWNKHGICSES 120

Query: 121 KFDEAKYFQTAINMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKA 180
           KFD+A YFQTAINMRH IDLLSV+RTGGV PNGASKAKQRVE+ ISSHFGKDPILRCK A
Sbjct: 121 KFDQAAYFQTAINMRHSIDLLSVMRTGGVIPNGASKAKQRVESVISSHFGKDPILRCKTA 180

Query: 181 SNGQVLLTEIVMCFDDDGVTLINCNKARSNCAGSFIF 217
           SNGQVLLTE+VMCFDDDGVTL+NCNKARSNCA SFIF
Sbjct: 181 SNGQVLLTEVVMCFDDDGVTLLNCNKARSNCATSFIF 216

[Close]

BLAST of CsGy3G036460 vs. NCBI nr
Match: XP_022934270.1 (ribonuclease MC-like [Cucurbita moschata])

HSP 1 Score: 355 bits (911), Expect = 3.11e-122
Identity = 161/208 (77.40%), Postives = 177/208 (85.10%), Query Frame = 0


Query: 10  VFFVFVLTILFPIVKSQTFDDFWFVQQWPPAICTLQSGRCVGRGTRSFTIHGLWPQKGGR 69
           +F +F   IL PI KSQ FDDFWFVQQWPP +C LQSGRCVGRGT+SFTIHGLWPQK G 
Sbjct: 8   IFLLFAFAILIPIAKSQPFDDFWFVQQWPPNVCALQSGRCVGRGTKSFTIHGLWPQKRGN 67

Query: 70  SVTNCTGNQFDFTKIAHLENDLNVVWPNVVTGNNKFFWGHEWNKHGICSESKFDEAKYFQ 129
           SVTNC G  FDF +IAHLENDLNVVWPN++TGN+K+FWGHEWN HGICSES+FDEA YFQ
Sbjct: 68  SVTNCPGTSFDFNQIAHLENDLNVVWPNLITGNHKWFWGHEWNTHGICSESRFDEAAYFQ 127

Query: 130 TAINMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKASNGQVLLTE 189
           T+IN+RH IDLLS LR  GV PNGASK+KQRVE AI  HF  +P+LRCK ASNGQVLLTE
Sbjct: 128 TSINLRHNIDLLSALRVQGVVPNGASKSKQRVENAIIKHFQNNPVLRCKTASNGQVLLTE 187

Query: 190 IVMCFDDDGVTLINCNKARSNCAGSFIF 217
           IVMC DDDGVTLINCN A+SNCA SFIF
Sbjct: 188 IVMCIDDDGVTLINCNLAKSNCANSFIF 215

[Close]

BLAST of CsGy3G036460 vs. NCBI nr
Match: XP_023528964.1 (ribonuclease MC-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 354 bits (909), Expect = 6.27e-122
Identity = 159/208 (76.44%), Postives = 179/208 (86.06%), Query Frame = 0


Query: 10  VFFVFVLTILFPIVKSQTFDDFWFVQQWPPAICTLQSGRCVGRGTRSFTIHGLWPQKGGR 69
           +F +FV  IL P  KSQ FDDFWFVQQWPP +C LQSGRC+GRGT SFTIHGLWPQKGG 
Sbjct: 8   IFLLFVFAILLPRTKSQPFDDFWFVQQWPPNVCALQSGRCIGRGTNSFTIHGLWPQKGGN 67

Query: 70  SVTNCTGNQFDFTKIAHLENDLNVVWPNVVTGNNKFFWGHEWNKHGICSESKFDEAKYFQ 129
           SVTNC G  FDF +I+HLENDLN+VWPN++TGN+K+FWGHEWN HGICSESK+DE  YFQ
Sbjct: 68  SVTNCPGTSFDFNQISHLENDLNIVWPNLITGNHKWFWGHEWNTHGICSESKYDEKAYFQ 127

Query: 130 TAINMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKASNGQVLLTE 189
           T+IN+RH I+LLS LR  GV PNGASK+KQRVE+AI SHF K+P+LRCK ASNGQVLLTE
Sbjct: 128 TSINLRHNINLLSALRVQGVVPNGASKSKQRVESAIISHFKKNPVLRCKTASNGQVLLTE 187

Query: 190 IVMCFDDDGVTLINCNKARSNCAGSFIF 217
           IVMC DDDGVTLINCN A+SNCA SFIF
Sbjct: 188 IVMCIDDDGVTLINCNLAKSNCANSFIF 215

[Close]

BLAST of CsGy3G036460 vs. ExPASy TrEMBL
Match: A0A0A0LC37 (Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G825050 PE=3 SV=1)

HSP 1 Score: 458 bits (1179), Expect = 2.26e-163
Identity = 216/217 (99.54%), Postives = 217/217 (100.00%), Query Frame = 0


Query: 1   MEKTKSVDVVFFVFVLTILFPIVKSQTFDDFWFVQQWPPAICTLQSGRCVGRGTRSFTIH 60
           MEKTKSVDVVFFVFVLTILFPIVKSQTFDDFWFVQQWPPA+CTLQSGRCVGRGTRSFTIH
Sbjct: 1   MEKTKSVDVVFFVFVLTILFPIVKSQTFDDFWFVQQWPPAVCTLQSGRCVGRGTRSFTIH 60

Query: 61  GLWPQKGGRSVTNCTGNQFDFTKIAHLENDLNVVWPNVVTGNNKFFWGHEWNKHGICSES 120
           GLWPQKGGRSVTNCTGNQFDFTKIAHLENDLNVVWPNVVTGNNKFFWGHEWNKHGICSES
Sbjct: 61  GLWPQKGGRSVTNCTGNQFDFTKIAHLENDLNVVWPNVVTGNNKFFWGHEWNKHGICSES 120

Query: 121 KFDEAKYFQTAINMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKA 180
           KFDEAKYFQTAINMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKA
Sbjct: 121 KFDEAKYFQTAINMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKA 180

Query: 181 SNGQVLLTEIVMCFDDDGVTLINCNKARSNCAGSFIF 217
           SNGQVLLTEIVMCFDDDGVTLINCNKARSNCAGSFIF
Sbjct: 181 SNGQVLLTEIVMCFDDDGVTLINCNKARSNCAGSFIF 217

[Close]

BLAST of CsGy3G036460 vs. ExPASy TrEMBL
Match: A0A1S3B8Q3 (Ribonuclease T(2) OS=Cucumis melo OX=3656 GN=LOC103486996 PE=3 SV=1)

HSP 1 Score: 427 bits (1098), Expect = 4.91e-151
Identity = 203/217 (93.55%), Postives = 209/217 (96.31%), Query Frame = 0


Query: 1   MEKTKSVDVVFFVFVLTILFPIVKSQTFDDFWFVQQWPPAICTLQSGRCVGRGTRSFTIH 60
           MEKTK  DVVFFVFVLTILFPIVKSQTFD+FWFVQQWPPAICTLQSGRCVGRGTRSFTIH
Sbjct: 1   MEKTKR-DVVFFVFVLTILFPIVKSQTFDNFWFVQQWPPAICTLQSGRCVGRGTRSFTIH 60

Query: 61  GLWPQKGGRSVTNCTGNQFDFTKIAHLENDLNVVWPNVVTGNNKFFWGHEWNKHGICSES 120
           GLWPQKGGRSVTNC+GN FDFTKIAHLE+DLNVVWPNVVTGNNKFFWGHEWNKHGICSES
Sbjct: 61  GLWPQKGGRSVTNCSGNSFDFTKIAHLESDLNVVWPNVVTGNNKFFWGHEWNKHGICSES 120

Query: 121 KFDEAKYFQTAINMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKA 180
           KFD+AKYFQTAINMRH IDLLSVLRTGG+ PNGASKAKQRVETAISSHF KDPILRCK A
Sbjct: 121 KFDQAKYFQTAINMRHSIDLLSVLRTGGIVPNGASKAKQRVETAISSHFAKDPILRCKTA 180

Query: 181 SNGQVLLTEIVMCFDDDGVTLINCNKARSNCAGSFIF 217
           SNGQVLLTEIV+CFDDDGVTLINCNKARSNCA SFIF
Sbjct: 181 SNGQVLLTEIVICFDDDGVTLINCNKARSNCARSFIF 216

[Close]

BLAST of CsGy3G036460 vs. ExPASy TrEMBL
Match: A0A6J1F776 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111441482 PE=3 SV=1)

HSP 1 Score: 355 bits (911), Expect = 1.51e-122
Identity = 161/208 (77.40%), Postives = 177/208 (85.10%), Query Frame = 0


Query: 10  VFFVFVLTILFPIVKSQTFDDFWFVQQWPPAICTLQSGRCVGRGTRSFTIHGLWPQKGGR 69
           +F +F   IL PI KSQ FDDFWFVQQWPP +C LQSGRCVGRGT+SFTIHGLWPQK G 
Sbjct: 8   IFLLFAFAILIPIAKSQPFDDFWFVQQWPPNVCALQSGRCVGRGTKSFTIHGLWPQKRGN 67

Query: 70  SVTNCTGNQFDFTKIAHLENDLNVVWPNVVTGNNKFFWGHEWNKHGICSESKFDEAKYFQ 129
           SVTNC G  FDF +IAHLENDLNVVWPN++TGN+K+FWGHEWN HGICSES+FDEA YFQ
Sbjct: 68  SVTNCPGTSFDFNQIAHLENDLNVVWPNLITGNHKWFWGHEWNTHGICSESRFDEAAYFQ 127

Query: 130 TAINMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKASNGQVLLTE 189
           T+IN+RH IDLLS LR  GV PNGASK+KQRVE AI  HF  +P+LRCK ASNGQVLLTE
Sbjct: 128 TSINLRHNIDLLSALRVQGVVPNGASKSKQRVENAIIKHFQNNPVLRCKTASNGQVLLTE 187

Query: 190 IVMCFDDDGVTLINCNKARSNCAGSFIF 217
           IVMC DDDGVTLINCN A+SNCA SFIF
Sbjct: 188 IVMCIDDDGVTLINCNLAKSNCANSFIF 215

[Close]

BLAST of CsGy3G036460 vs. ExPASy TrEMBL
Match: A0A6J1J1Y7 (Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111481960 PE=3 SV=1)

HSP 1 Score: 352 bits (903), Expect = 2.49e-121
Identity = 160/208 (76.92%), Postives = 175/208 (84.13%), Query Frame = 0


Query: 10  VFFVFVLTILFPIVKSQTFDDFWFVQQWPPAICTLQSGRCVGRGTRSFTIHGLWPQKGGR 69
           +F +F   IL PI KSQ FDDFWFVQQWPP +C LQ GRCVGRGT SFTIHGLWPQK G 
Sbjct: 8   IFLLFAFAILIPIAKSQPFDDFWFVQQWPPNVCALQLGRCVGRGTNSFTIHGLWPQKRGN 67

Query: 70  SVTNCTGNQFDFTKIAHLENDLNVVWPNVVTGNNKFFWGHEWNKHGICSESKFDEAKYFQ 129
           SVTNC G  FDF +IAHLENDLNVVWPN++TGN+K+FWGHEWN HGICSES+FDEA YFQ
Sbjct: 68  SVTNCPGTSFDFNQIAHLENDLNVVWPNLITGNHKWFWGHEWNTHGICSESRFDEAAYFQ 127

Query: 130 TAINMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKASNGQVLLTE 189
           T+IN+RH IDLLS LR  GV PNGASK+KQRVE AI  HF  +P+LRCK ASNGQVLLTE
Sbjct: 128 TSINLRHNIDLLSALRVQGVVPNGASKSKQRVENAIIKHFKNNPVLRCKTASNGQVLLTE 187

Query: 190 IVMCFDDDGVTLINCNKARSNCAGSFIF 217
           IVMC DDDGVTLINCN A+SNCA SFIF
Sbjct: 188 IVMCIDDDGVTLINCNLAKSNCANSFIF 215

[Close]

BLAST of CsGy3G036460 vs. ExPASy TrEMBL
Match: A0A6J1FBD8 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111442477 PE=3 SV=1)

HSP 1 Score: 351 bits (900), Expect = 7.14e-121
Identity = 158/208 (75.96%), Postives = 178/208 (85.58%), Query Frame = 0


Query: 10  VFFVFVLTILFPIVKSQTFDDFWFVQQWPPAICTLQSGRCVGRGTRSFTIHGLWPQKGGR 69
           +F +FV   L P+ KSQ FDDFWFVQQWPP +C LQSGRCVGRGT SFTIHGLWPQKGG 
Sbjct: 8   IFLLFVFVSLLPMAKSQPFDDFWFVQQWPPNVCALQSGRCVGRGTNSFTIHGLWPQKGGN 67

Query: 70  SVTNCTGNQFDFTKIAHLENDLNVVWPNVVTGNNKFFWGHEWNKHGICSESKFDEAKYFQ 129
           SVTNC G  FDF +I+HLENDLN+VWPN++TGN+K+FWGHEWN HGICSESKFD+  YFQ
Sbjct: 68  SVTNCPGTSFDFNQISHLENDLNIVWPNLITGNHKWFWGHEWNTHGICSESKFDQKAYFQ 127

Query: 130 TAINMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKASNGQVLLTE 189
           T+IN+RH I+LLS LR  GV PNGASK+KQRVE+AI SHF K P+LRCK ASNGQVLLTE
Sbjct: 128 TSINLRHNINLLSALRVQGVVPNGASKSKQRVESAIISHFKKIPVLRCKTASNGQVLLTE 187

Query: 190 IVMCFDDDGVTLINCNKARSNCAGSFIF 217
           IVMC DDDGVTLINCN A+SNCA +FIF
Sbjct: 188 IVMCIDDDGVTLINCNIAKSNCANNFIF 215

[Close]

BLAST of CsGy3G036460 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 145.6 bits (366), Expect = 4.7e-35
Identity = 73/201 (36.32%), Postives = 107/201 (53.23%), Query Frame = 0


Query: 16  LTILFPIV-----KSQTFDDFWFVQQWPPAICTLQSGRCV---GRGTRSFTIHGLWPQ-K 75
           L ++ P V      S+ FD F+FVQQWP + C  Q   C    G+    F IHGLWP  K
Sbjct: 13  LLVILPSVFSASSSSEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGIHGLWPNYK 72

Query: 76  GGRSVTNCTGNQ-FDFTKIAHLENDLNVVWPNVV--TGNNKFFWGHEWNKHGICSESKFD 135
            G   +NC  ++ FD + I+ L   +   WP +   +G+ + FW HEW KHG CSES  D
Sbjct: 73  DGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSESVID 132

Query: 136 EAKYFQTAINMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKASNG 195
           + +YFQTA+N++   +LL  L   G+ P+G S + + +  +I    G  P + C +  +G
Sbjct: 133 QHEYFQTALNLKQKTNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVECNRDGSG 192

Query: 196 QVLLTEIVMCFDDDGVTLINC 205
              L ++ +C D  G  LI C
Sbjct: 193 NSQLYQVYLCVDRSGSGLIEC 213

[Close]

BLAST of CsGy3G036460 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 132.5 bits (332), Expect = 4.1e-31
Identity = 71/205 (34.63%), Postives = 102/205 (49.76%), Query Frame = 0


Query: 7   VDVVFFVFVLTILFPIVKSQTFDDFWFVQQWPPAICTLQSGRCV---GRGTRSFTIHGLW 66
           V+V+  + V   LF     + FD F+FV QWP A C      C    G+    F IHGLW
Sbjct: 7   VNVLLKLLVFQGLFVSRPQEDFDFFYFVLQWPGAYCDTSRACCYPTSGKPAADFGIHGLW 66

Query: 67  PQ-KGGRSVTNC-TGNQFDFTKIAHLENDLNVVWPNVVTGNNK--FFWGHEWNKHGICSE 126
           P   GG   +NC   +QFD ++I+ L + L   WP +   +N+   FW HEW KHG CSE
Sbjct: 67  PNYNGGSWPSNCDPDSQFDRSQISDLVSSLKKNWPTLSCPSNEGFNFWEHEWEKHGTCSE 126

Query: 127 SKFDEAKYFQTAINMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKK 186
           S  D+ +YF+ A+ ++   +LL +L+  G+ P+       ++  AI    G  P + C K
Sbjct: 127 SVMDQHEYFENALKLKQKANLLQILKNSGINPDDGFYNLDKITNAIKDGIGFTPGIECNK 186

Query: 187 ASNGQVLLTEIVMCFDDDGVTLINC 205
                  L +I +C D  G   I C
Sbjct: 187 DPERNAQLHQIYICVDTSGTEFIEC 211

[Close]

BLAST of CsGy3G036460 vs. TAIR 10
Match: AT1G26820.1 (ribonuclease 3 )

HSP 1 Score: 129.4 bits (324), Expect = 3.5e-30
Identity = 71/203 (34.98%), Postives = 102/203 (50.25%), Query Frame = 0


Query: 11  FFVFVLTILFPIVKS--QTFDDFWFVQQWPPAICTLQSGRC---VGRGTRSFTIHGLWPQ 70
           FF+F+L +    V+S  Q FD F+FV QWP A C  +   C    G+    F IHGLWP 
Sbjct: 3   FFIFILALQQLYVQSFAQDFDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLWPN 62

Query: 71  -KGGRSVTNCT-GNQFDFTKIAHLENDLNVVWPNVVTGNN--KFFWGHEWNKHGICSESK 130
            K G    NC   ++FD  +++ L +DL   WP +   +N    FW HEW KHG C+ES+
Sbjct: 63  YKTGGWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESE 122

Query: 131 FDEAKYFQTAINMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKAS 190
            D+  YF+  + ++   +LL  L   G+ P+      + +E  I    G  P + C K S
Sbjct: 123 LDQHDYFEAGLKLKQKANLLHALTNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECNKDS 182

Query: 191 NGQVLLTEIVMCFDDDGVTLINC 205
           +    L +I +C D      INC
Sbjct: 183 SHNSQLYQIYLCVDTSASKFINC 205

[Close]

BLAST of CsGy3G036460 vs. TAIR 10
Match: AT1G14210.1 (Ribonuclease T2 family protein )

HSP 1 Score: 119.8 bits (299), Expect = 2.8e-27
Identity = 66/195 (33.85%), Postives = 91/195 (46.67%), Query Frame = 0


Query: 22  IVKSQT---FDDFWFVQQWPPAICTLQSGRC---VGRGTRSFTIHGLWPQ-KGGRSVTNC 81
           +  SQT   F+ F++V  WP AIC  Q G C    G     F IHGLWPQ   G     C
Sbjct: 17  VSSSQTEPDFNFFYWVNYWPGAICDSQKGCCPPTKGNTASDFIIHGLWPQFNNGTWPAFC 76

Query: 82  -TGNQFDFTKIAHLENDLNVVWPN----VVTGNNKFFWGHEWNKHGICSESKFDEAKYFQ 141
              N FD +KI+ L   +   W          N    W HEWNKHG C +S FD+  YF+
Sbjct: 77  DQTNLFDISKISDLVCQMEKKWTEWGVWACPSNETNLWEHEWNKHGTCVQSIFDQHSYFR 136

Query: 142 TAINMRHGIDLLSVLRTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKASNGQVLLTE 201
           T +  +H + LL++L   G+ PN    +   ++ AI    G  P + C +   G   L +
Sbjct: 137 TNLKFKHKVHLLNILIQKGIKPNDGFYSLDEIKNAIKCAIGFAPGIECNEDVKGNKQLFQ 196

Query: 202 IVMCFDDDGVTLINC 205
           I +C D+     + C
Sbjct: 197 IYICLDNYAKEFVEC 211

[Close]

BLAST of CsGy3G036460 vs. TAIR 10
Match: AT2G39780.1 (ribonuclease 2 )

HSP 1 Score: 86.7 bits (213), Expect = 2.6e-17
Identity = 61/187 (32.62%), Postives = 82/187 (43.85%), Query Frame = 0


Query: 28  FDDFWFVQQWPPAI------CTLQSGRCVGR-GTRSFTIHGLWPQ-KGGRSVTNCTGNQF 87
           FD F    QWP         C  ++  C G      FTIHGLWP    G   + C  + F
Sbjct: 32  FDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPTQFTIHGLWPDYNDGSWPSCCYRSDF 91

Query: 88  DFTKIAHLENDLNVVWPNV-------VTGNNKFFWGHEWNKHGICSESKF-DEAKYFQTA 147
              +I+ L + L   WP++         G    FWGHEW KHG CS   F DE  YF T 
Sbjct: 92  KEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFWGHEWEKHGTCSSPVFHDEYNYFLTT 151

Query: 148 INMRHGIDLLSVL-RTGGVGPNGASKAKQRVETAISSHFGKDPILRCKKASNGQVLLTEI 198
           +N+    ++  VL + G V  N        + TAI + F   P + CK+ +     + EI
Sbjct: 152 LNLYLKHNVTDVLYQAGYVASNSEKYPLGGIVTAIQNAFHITPEVVCKRDA-----IDEI 211

The following BLAST results are available for this feature:

BLAST of CsGy3G036460 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P23540	1.3e-58	52.33	Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
P42813	6.6e-34	36.32	Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1	[more]
P80022	7.6e-30	31.92	Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2	[more]
P42815	4.9e-29	34.98	Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1	[more]
P80196	5.1e-26	32.08	Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2	[more]

BLAST of CsGy3G036460 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004147499.1	4.67e-163	99.54	ribonuclease MC [Cucumis sativus] >KGN59560.1 hypothetical protein Csa_002553 [C...	[more]
XP_008443402.1	1.01e-150	93.55	PREDICTED: ribonuclease MC-like [Cucumis melo]	[more]
XP_038905534.1	2.46e-142	87.10	ribonuclease MC-like [Benincasa hispida]	[more]
XP_022934270.1	3.11e-122	77.40	ribonuclease MC-like [Cucurbita moschata]	[more]
XP_023528964.1	6.27e-122	76.44	ribonuclease MC-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of CsGy3G036460 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LC37	2.26e-163	99.54	Ribonuclease T(2) OS=Cucumis sativus OX=3659 GN=Csa_3G825050 PE=3 SV=1	[more]
A0A1S3B8Q3	4.91e-151	93.55	Ribonuclease T(2) OS=Cucumis melo OX=3656 GN=LOC103486996 PE=3 SV=1	[more]
A0A6J1F776	1.51e-122	77.40	Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111441482 PE=3 SV=1	[more]
A0A6J1J1Y7	2.49e-121	76.92	Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111481960 PE=3 SV=1	[more]
A0A6J1FBD8	7.14e-121	75.96	Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111442477 PE=3 SV=1	[more]

BLAST of CsGy3G036460 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G02990.1	4.7e-35	36.32	ribonuclease 1	[more]
AT1G14220.1	4.1e-31	34.63	Ribonuclease T2 family protein	[more]
AT1G26820.1	3.5e-30	34.98	ribonuclease 3	[more]
AT1G14210.1	2.8e-27	33.85	Ribonuclease T2 family protein	[more]
AT2G39780.1	2.6e-17	32.62	ribonuclease 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 29..204 e-value: 7.5E-44 score: 150.1
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 17..212
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 28..215 e-value: 1.2E-55 score: 190.5
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 28..217
None	No IPR available	PANTHER	PTHR11240:SF49	RIBONUCLEASE 3	coord: 17..212
IPR033130	Ribonuclease T2, His active site 2	PROSITE	PS00531	RNASE_T2_2	coord: 106..117
IPR018188	Ribonuclease T2, His active site 1	PROSITE	PS00530	RNASE_T2_1	coord: 57..64
IPR033697	Ribonuclease T2, eukaryotic	CDD	cd01061	RNase_T2_euk	coord: 28..216 e-value: 4.90803E-70 score: 210.266

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsGy3G036460.2	CsGy3G036460.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006401	RNA catabolic process
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding