CsGy3G035930 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy3G035930
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
DescriptionHydroxymethylglutaryl-CoA lyase
LocationGy14Chr3: 34290001 .. 34295391 (-)
RNA-Seq ExpressionCsGy3G035930
SyntenyCsGy3G035930
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAAGCTGCAAAGCTCTCTGCTAAATTCACTCGACAATCTTTTTCTTGGCGTCATGCTTATAATCTCTCCATGGAAACTCCCACCCCATTTCTTGTTTCACCTTCCGCCACAGATGTAGGCGTCAGCTCCATCAGAACCCCTGGCATACAACGAGAATTTTATAATTGCAGGTATAGTCGAAACGATTCTGATTAATGATTATAGGGAGAATTGCTCGTACCCTTTTGATTTTTAAACTTTTGCTTTGTATTAAACCTCTTGATTATCGTTGCAATAGCATTTGCGGAAAATGTGCTGTAGAATTCTTTCAGTCCTTTAGGGGATGAGTCATGGAATATGCATCGTGAAATTAGGGCATTTTTGTTTTCTAAATTTATGATGAGAAATCTACTTCATCTACTTTTGTTAGGGTCCTTTCAAGAGTAGAGCTAATAATTTGATTGGGTTTGGAGAGAATAATTTGATTAATTTCTACCTTCAGTCTTCGCTTTCAGTTTCTATACACGGTGAATGTAGGAATTATCCCTTTGGATTTAATAGAGATCAAGAGGTCCCTCGTATGCTATACCTGGCTAGGTTTATTGGACTTTCTTAGTAACGGCACAGAACTTGTGTAATAGAATCTAGAAGTATTATATATTTGAATTAACTGTGGTATCTCTTTTTTTTTTATTTTCTTTAAGTCTCGTATTAAGTTTGAATGTAATGCAACATTCCATTTCTTTCTCTCCAGTCAACAAGATGGAAGAGATGCAGTCCTTTCGAGAGAAAATAAGGGGGGATTGTCCCAGGTGATGTTCTAAAATGGATTTTTCTTAGTATAAGATGTAAAATCTGGGAGTTGAGTAATCCTTATCACCTTGAGCATTTACCGAATTGTTATGTTTCCCGTCAAGATCTTTGTCCTCTTATTTATATTCTTGTATGATATGATTTGTAACAAGTCTTTATTTTATTTTATTTTTCTGTTTTTTTTGTTTGGTTTTTTTCCTCTTCTTTTTTTTGTACTGTAATCTTAATCTTAACTATTGTTTTCGATTATAATGGTTATTTAATATATTCAGTGGTTTCTGTTTCTTATATAAAAGAAAAGAAAAGAAAAGAAATAAATATATTGAATTTTTTGATGAAAGTTTTTTTCTTCCTTTGTAGTATTTATGCCGTATTCCGCCATTTGTGAAGATTGTTGAAGTTGGTCCAAGAGATGGACTACAAAATGAAAAGCACATTGTACCTACTGCTGTTAAGGTTGATTTAATAAAGATGCTAGTCTCTTCTGGCTTGCCTGTTGTTGAAGCCACGAGTTTTGTTTCTCCAAAATGGGTACCGCAGGTAAGGTTGCTTGATACTATGAAAATATTGGTTGGTATATTGTTTCTATTCCTGAAACAGTTACCTTGTCTCATTCTTCCTTCTCCTGGCCTTCAACTTTTCATGTGCTCTGTCTTCGTGTTTGAATTTCTTGTACGTGTACGTATTAATCTCTTTTCCTCATATTTTCCTCAGTTAGCAGATGCAAAGGAAGTTATGGAGGGAATTCGAAATGTTGAAGGTGCTCGATTTCCTGTGTTGACCCCAAATCTTAAAGTACGAGCTTTAATACACAAATGCAAAATTAGCTGTATTAAAAAAAATACAAAACAAAATTAGGACTAATCTGGAAGGCATTTCGTGATTTTTGGACTTATGAGATTTTTTTACTTCTTTATCTAGGGTTTTGAAGCAGCTGTAGCAGCTGGAGCAAAGGAAGTAGCCATCTTTGCTTCAGCGTCTGAATCCTTCTCGAAGTCAAATATCAATTGCAGCATAGAAGAAAGTCTTCATCGCTACCGCCAAGTTGCTGATGCGGCAAGAAAGCACTCACTTCCAGTTCGTGGGTAGGTATATGAACCAGAAAACTGAATATTTTCTTTTCAACAATCATACATCTGGTTTGATTTTGTTACTTTTAGTGCTGAGGCTATAATACTTCGACCCCTTTATGTCTACAAAATAATACAGTTGATATGCTCATAAGCCAACAGACATCATCTATAAAATGTCTACCTCTATCTGGCATGAGAGCACTTTGGTTGGTTTAAATGATCCGATCCAATACAAATGGTACGCAAGAATGTTTGACATAATTTTGTAGATCCGTGATCACATTACATATACCTGTTGAATGAAAGACAGATTCAATTCATTAATATTGGGCTTAGGGAGTTTGATTGAAGTATCACGAAATGTTCATTGAGATCAACACCTCTATGAAAGCACCCTCTACTCTCGATTTCATCTCAGAATTTTCAGAACTTTTGATTGAACACTGCAAGCAGACCATCTAGATTTGAACCTTCAGTAGGGCACATGTTACTACAATTGGAGGTTCCTCTCCATCAATCCAAATTGGTGTTCCATTCCTCTTTTTATTAGAAACAAACTGTCATTGGGAAGAGAAACAAAATAATAAAAGAGGCATACGGACAAGCCAACCCTAAAAAGGAGCCAACCTGATGTAAATGAGTCCAAAAAAGTATGAATGAGAACACGTAACGGTTAATTACTAATTATAAAGTCCTTACACACTAAAGCCCAAAGGGAGGTACGGACCCTAGCGAGGAGGGATCTAACTTTCTCCTCAGATCTCTTAACTCCTATGAGAACTCTATTGGTTCCTCTCACCCTAAAGACCTCAAAACATTTTCATCTATTTTGTAACTTTTTTAGGAACTCTTGTGTTTTCAACAGCTTTCATCATTTGATTTGATGATGCTCTGCTGTGAAATCAATCCTCCATTAACCTTTGCTTTTAGCTGGTTTTCTTCGTTTCAAAATATTTTCTAGCTTTCTCAGGACCTGAGGTGAAGGTGCGTTGGTTTAACATCTGAAGTGTTCTTCTTTGGAAAGTGTGGTTGGAATGTCTAGGAGATGTTTCAGAGACGAGGATAATTCAAGAGAGCAAATTTTCGAATAACTTATTTATTTTTTGTTGTTTTAGTTTCTAAATTCTGAAAATGTTTGTTGATCTTTGTAGAGCAACTTTGGCGTTCTTTGTAGTCTCTTTCTTATTAAAAAAAAAAAGAAAAAAAGAAGAGATAATCATAGTCGGACGTTATTACCAAAATATTTTTTCATGTTTCAATTGCATCTGCCTCCTTTTACTATTAGAGGTGGGAGGGTGTCCAGCCCTCCCATCTTATATGTGTCTTATAAATTAAAATGTAATTATGTATTTGTCGATAATTATATGTTTATTTAGATTTAAAATCTTTTAGAGTGAGTGATATGATTCTCTTTTTATATTTTGAAAATGTCTCCTATAAACATGTTAGATATGTATCATGTGTTGTGGGCTGTCCCATTGAAGGAATGGTGCCGGCATCAAATGTAGCATACGTTGCTAAGGAGCTTTACAGTATGGGATGCTCAGAGATTTCCCTTGGTGATACAATTGGTGTTGGTACTCCAGGTAACCACTAGGACGATCATCTTTCAAGCAATTTTCTGTACTTTGCTCAGTGAGTGAGATCAGGAGGGGCTCTCGAATCTCCATGACTTGTACAATTTTTGTCATATTTTATCTTACTTTTAAGAGAATTTCCCGTAGAATACTTATATTCTATTCAATTCGTATAATCATCTTATTGAAAGCATCAAGTTATTAACTTATAAAAAAGTACCATTATCTAGATATTACATAATAAATTTTTTTGGTCAGAAATAACTTTAAAAAAGCATCAATTCTTGCTTTCTTCTTTTCCTTCTTGGTCTTCTTCTTCTGCTGCTTCTTCTTCTTCTTCGCCTCCTCCTCCTTCTGCTTCTTCTTTAACTTTAGTTTTTTGCAACTGCAGGAACTGTGATTCCAATGCTTGAATCTGTAGCTAATGTTGTTCCAACTGAAAATCTTGCTGTTCATTTCCATGATACTTACGGGCAAGCTCTGTCCAATATTTTAGTCTCACTCCAGGTATTCTCTTCTTTTCCATCTTGGTTTTAATTGCTCGGAACCTACAAGTCCTCAAGCAATATATACCAATGAATTGGTATTTATTTGGCGAGTATCTGATAGAATCACAACCACTGTATATATTCAGAATCCAAGGCACAATTTAGAGACGTTAAAGAGACCTTATCTTTGGTACGCTACCTTGCAGTTGCACTTAGCAAGAATCCTTGTACCCTATCCCTTTCTAGCGCCCTATATACTCCTAGGCAACCCACTCACACAACTATCTTATAGCAGCCCACAAATTGAAATTTTGTCGTCTTTTTAGACATGAAATTCAATGTTATGTTTAACAAACATTTTAACTTTAGAAAATGCCAAATAAAAAATGTTAAATACAACTTACTAGTATAAGAACTAGATCTTGAACAAGTATCACTGACTGATGTGTCAAATAGTAAAGGGAAGGTACCCATAGTACTGAAGATTTTCCCCTTTCACTTGCTTTCTCTTGGATATTGACTACCAAAGGCTTATCCTTATCCCTTGGCATCTAAAACTTGTTTGGGTTTTATTAGGAGGGAAAATATACAAAGCACCTAGTTACCAATATATACACACACACATATATGGGGGCGTTTGGTTAGTGATAAAAACAAAGAGTTGAGCTGAGTTGGCACTAATTATAGGGAAGTTATTGGGGGAGTTGACATGATTAACGATGTGAGAATTGTGAGTATTTTGGTGCAAAATGTGAGTTGATGTGAATAACAAGATAGTAAACCTCTTTAATTATTGGTGAACCAAACATATGATTGTTTTTTTTTACCCATGCAATCCAACTCTTCCAATTTATTCAAGTTACCAAACACCCTAGTTCAAAAAAGACAAAATGAAGACCTTGTCCTAGAACTTTGAAGCTTAGAACTAGATTTTTGTGAAAGAAGCAATGTTATAAAGTGAGGGTTAAACAGTTACGACTCCATTTTATAGTTGTATGGTTTTCTTTAAAAAATTAAGTTTATAAATACTACTCTCACTTCACCTATGAGTTACACTATTTAATTGTGATTGATAGTGAAAACGAATATGTGATTGTGATTGATAGTGAAAACAAATATGTTACACTTGCACGGAATGGACACAATGATGTTTAAAAATGAATGAAAAACATTTTATCTTATTATCAGATGGGGATTGGTGCGGTGGATTCTTCGGTTTCGGGTCTTGGGGGTTGTCCATATGCCAAGGGTGCTTCTGGAAACGTTGCAACTGAAGATGTTGTGTATATGCTGAATGGAATTGGGGCGAAAACCAATGTAGATCTTGGAAAGCTCATGTTGGCTGGAGATTTCATCTGCAAGCAGTTGGGGCGTCCTTCAGCTTCAAAAGTAGCAATTGCATTGCGGAAAGTTGCCTCCAACACCAATAAATGCACAGCCACTGCATCCAAGTTATGA

mRNA sequence

ATGCAAGCTGCAAAGCTCTCTGCTAAATTCACTCGACAATCTTTTTCTTGGCGTCATGCTTATAATCTCTCCATGGAAACTCCCACCCCATTTCTTGTTTCACCTTCCGCCACAGATGTAGGCGTCAGCTCCATCAGAACCCCTGGCATACAACGAGAATTTTATAATTGCAGTCAACAAGATGGAAGAGATGCAGTCCTTTCGAGAGAAAATAAGGGGGGATTGTCCCAGTATTTATGCCGTATTCCGCCATTTGTGAAGATTGTTGAAGTTGGTCCAAGAGATGGACTACAAAATGAAAAGCACATTGTACCTACTGCTGTTAAGGTTGATTTAATAAAGATGCTAGTCTCTTCTGGCTTGCCTGTTGTTGAAGCCACGAGTTTTGTTTCTCCAAAATGGGTACCGCAGTTAGCAGATGCAAAGGAAGTTATGGAGGGAATTCGAAATGTTGAAGGTGCTCGATTTCCTGTGTTGACCCCAAATCTTAAAGGTTTTGAAGCAGCTGTAGCAGCTGGAGCAAAGGAAGTAGCCATCTTTGCTTCAGCGTCTGAATCCTTCTCGAAGTCAAATATCAATTGCAGCATAGAAGAAAGTCTTCATCGCTACCGCCAAGTTGCTGATGCGGCAAGAAAGCACTCACTTCCAGTTCGTGGATATGTATCATGTGTTGTGGGCTGTCCCATTGAAGGAATGGTGCCGGCATCAAATGTAGCATACGTTGCTAAGGAGCTTTACAGTATGGGATGCTCAGAGATTTCCCTTGGTGATACAATTGGTGTTGGTACTCCAGGAACTGTGATTCCAATGCTTGAATCTGTAGCTAATGTTGTTCCAACTGAAAATCTTGCTGTTCATTTCCATGATACTTACGGGCAAGCTCTGTCCAATATTTTAGTCTCACTCCAGATGGGGATTGGTGCGGTGGATTCTTCGGTTTCGGGTCTTGGGGGTTGTCCATATGCCAAGGGTGCTTCTGGAAACGTTGCAACTGAAGATGTTGTGTATATGCTGAATGGAATTGGGGCGAAAACCAATGTAGATCTTGGAAAGCTCATGTTGGCTGGAGATTTCATCTGCAAGCAGTTGGGGCGTCCTTCAGCTTCAAAAGTAGCAATTGCATTGCGGAAAGTTGCCTCCAACACCAATAAATGCACAGCCACTGCATCCAAGTTATGA

Coding sequence (CDS)

ATGCAAGCTGCAAAGCTCTCTGCTAAATTCACTCGACAATCTTTTTCTTGGCGTCATGCTTATAATCTCTCCATGGAAACTCCCACCCCATTTCTTGTTTCACCTTCCGCCACAGATGTAGGCGTCAGCTCCATCAGAACCCCTGGCATACAACGAGAATTTTATAATTGCAGTCAACAAGATGGAAGAGATGCAGTCCTTTCGAGAGAAAATAAGGGGGGATTGTCCCAGTATTTATGCCGTATTCCGCCATTTGTGAAGATTGTTGAAGTTGGTCCAAGAGATGGACTACAAAATGAAAAGCACATTGTACCTACTGCTGTTAAGGTTGATTTAATAAAGATGCTAGTCTCTTCTGGCTTGCCTGTTGTTGAAGCCACGAGTTTTGTTTCTCCAAAATGGGTACCGCAGTTAGCAGATGCAAAGGAAGTTATGGAGGGAATTCGAAATGTTGAAGGTGCTCGATTTCCTGTGTTGACCCCAAATCTTAAAGGTTTTGAAGCAGCTGTAGCAGCTGGAGCAAAGGAAGTAGCCATCTTTGCTTCAGCGTCTGAATCCTTCTCGAAGTCAAATATCAATTGCAGCATAGAAGAAAGTCTTCATCGCTACCGCCAAGTTGCTGATGCGGCAAGAAAGCACTCACTTCCAGTTCGTGGATATGTATCATGTGTTGTGGGCTGTCCCATTGAAGGAATGGTGCCGGCATCAAATGTAGCATACGTTGCTAAGGAGCTTTACAGTATGGGATGCTCAGAGATTTCCCTTGGTGATACAATTGGTGTTGGTACTCCAGGAACTGTGATTCCAATGCTTGAATCTGTAGCTAATGTTGTTCCAACTGAAAATCTTGCTGTTCATTTCCATGATACTTACGGGCAAGCTCTGTCCAATATTTTAGTCTCACTCCAGATGGGGATTGGTGCGGTGGATTCTTCGGTTTCGGGTCTTGGGGGTTGTCCATATGCCAAGGGTGCTTCTGGAAACGTTGCAACTGAAGATGTTGTGTATATGCTGAATGGAATTGGGGCGAAAACCAATGTAGATCTTGGAAAGCTCATGTTGGCTGGAGATTTCATCTGCAAGCAGTTGGGGCGTCCTTCAGCTTCAAAAGTAGCAATTGCATTGCGGAAAGTTGCCTCCAACACCAATAAATGCACAGCCACTGCATCCAAGTTATGA

Protein sequence

MQAAKLSAKFTRQSFSWRHAYNLSMETPTPFLVSPSATDVGVSSIRTPGIQREFYNCSQQDGRDAVLSRENKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSGLPVVEATSFVSPKWVPQLADAKEVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIFASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAYVAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVANVVPTENLAVHFHDTYGQALSNILVSLQMGIGAVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGAKTNVDLGKLMLAGDFICKQLGRPSASKVAIALRKVASNTNKCTATASKL*
Homology
BLAST of CsGy3G035930 vs. ExPASy Swiss-Prot
Match: O81027 (Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=HMGCL PE=1 SV=2)

HSP 1 Score: 479.6 bits (1233), Expect = 3.5e-134
Identity = 254/387 (65.63%), Postives = 299/387 (77.26%), Query Frame = 0

Query: 10  FTRQSFSWRHAYNLSMETPTPF----LVSPSATDVGVSSIRTPGIQREFYNCSQQDGRDA 69
           F ++SF WR       E    F        ++T  G         ++++   S ++G   
Sbjct: 96  FAKESFPWRRHTRKLSEGEHMFRNISFAGRTSTVSGTLRESKSFKEQKYSTFSNENGTSH 155

Query: 70  VLSRENKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSGLPVVE 129
           + ++ +KG        IP FVKIVEVGPRDGLQNEK+IVPT+VKV+LI+ LVSSGLPVVE
Sbjct: 156 ISNKISKG--------IPKFVKIVEVGPRDGLQNEKNIVPTSVKVELIQRLVSSGLPVVE 215

Query: 130 ATSFVSPKWVPQLADAKEVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIFASASE 189
           ATSFVSPKWVPQLADAK+VM+ +  ++GAR PVLTPNLKGF+AAV+AGAKEVAIFASASE
Sbjct: 216 ATSFVSPKWVPQLADAKDVMDAVNTLDGARLPVLTPNLKGFQAAVSAGAKEVAIFASASE 275

Query: 190 SFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAYVAKEL 249
           SFS SNINC+IEESL RYR VA AA++HS+PVRGYVSCVVGCP+EG V  S VAYV KEL
Sbjct: 276 SFSLSNINCTIEESLLRYRVVATAAKEHSVPVRGYVSCVVGCPVEGPVLPSKVAYVVKEL 335

Query: 250 YSMGCSEISLGDTIGVGTPGTVIPMLESVANVVPTENLAVHFHDTYGQALSNILVSLQMG 309
           Y MGC EISLGDTIG+GTPG+V+PMLE+V  VVP + LAVHFHDTYGQAL+NILVSLQMG
Sbjct: 336 YDMGCFEISLGDTIGIGTPGSVVPMLEAVMAVVPADKLAVHFHDTYGQALANILVSLQMG 395

Query: 310 IGAVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGAKTNVDLGKLMLAGDFICKQLGR 369
           I  VDSS++GLGGCPYAKGASGNVATEDVVYMLNG+G  TNVDLGKL+ AGDFI K LGR
Sbjct: 396 ISIVDSSIAGLGGCPYAKGASGNVATEDVVYMLNGLGVHTNVDLGKLIAAGDFISKHLGR 455

Query: 370 PSASKVAIALRKVASNTNKCTATASKL 393
           P+ SK A+AL +      + TA ASK+
Sbjct: 456 PNGSKAAVALNR------RITADASKI 468

BLAST of CsGy3G035930 vs. ExPASy Swiss-Prot
Match: P35915 (Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Gallus gallus OX=9031 GN=HMGCL PE=1 SV=1)

HSP 1 Score: 396.7 bits (1018), Expect = 3.0e-109
Identity = 199/292 (68.15%), Postives = 237/292 (81.16%), Query Frame = 0

Query: 83  PPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSGLPVVEATSFVSPKWVPQLADAK 142
           P  VK+VEVGPRDGLQNEK +VPT VK+ LI ML  +GLPV+EATSFVSP+WVPQ+AD  
Sbjct: 3   PQRVKVVEVGPRDGLQNEKSVVPTPVKIRLIDMLSETGLPVIEATSFVSPRWVPQMADHA 62

Query: 143 EVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIFASASESFSKSNINCSIEESLHR 202
           EVM+GI  + G  +PVLTPNLKGF+AAVAAGAKEV+IF +ASE F+K NINCSIEESL R
Sbjct: 63  EVMQGINKLPGVSYPVLTPNLKGFQAAVAAGAKEVSIFGAASELFTKKNINCSIEESLER 122

Query: 203 YRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAYVAKELYSMGCSEISLGDTIGVG 262
           + +V +AAR  S+PVRGYVSCV+GCP EG + A+ VA V+K++YSMGC EISLGD IG+G
Sbjct: 123 FSEVMNAARAASIPVRGYVSCVLGCPYEGNISAAKVAEVSKKMYSMGCYEISLGDRIGIG 182

Query: 263 TPGTVIPMLESVANVVPTENLAVHFHDTYGQALSNILVSLQMGIGAVDSSVSGLGGCPYA 322
           TPG++  ML +V   VP   LAVH HDTYGQAL+NILV+LQMG+  VD+SV+GLGGCPYA
Sbjct: 183 TPGSMKEMLAAVMKEVPVGALAVHCHDTYGQALANILVALQMGVSVVDASVAGLGGCPYA 242

Query: 323 KGASGNVATEDVVYMLNGIGAKTNVDLGKLMLAGDFICKQLGRPSASKVAIA 375
           +GASGNVATED+VYMLNG+G  T VDL KLM  G FIC  L R + SKV+ A
Sbjct: 243 QGASGNVATEDLVYMLNGLGIHTGVDLQKLMDTGTFICNALNRRTNSKVSQA 294

BLAST of CsGy3G035930 vs. ExPASy Swiss-Prot
Match: P97519 (Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Rattus norvegicus OX=10116 GN=Hmgcl PE=2 SV=1)

HSP 1 Score: 391.7 bits (1005), Expect = 9.6e-108
Identity = 199/297 (67.00%), Postives = 236/297 (79.46%), Query Frame = 0

Query: 82  IPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSGLPVVEATSFVSPKWVPQLADA 141
           +P  VKIVEVGPRDGLQNEK IVPT VK+ LI ML  +GLPV+EATSFVSPKWVPQ+AD 
Sbjct: 29  LPKRVKIVEVGPRDGLQNEKSIVPTPVKIKLIDMLSEAGLPVIEATSFVSPKWVPQMADH 88

Query: 142 KEVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIFASASESFSKSNINCSIEESLH 201
            +V++GI+   G  +PVLTPN+KGFE AVAAGAKEV+IF +ASE F++ N+NCSIEES  
Sbjct: 89  SDVLKGIQKFPGINYPVLTPNMKGFEEAVAAGAKEVSIFGAASELFTRKNVNCSIEESFQ 148

Query: 202 RYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAYVAKELYSMGCSEISLGDTIGV 261
           R+  V  AAR  S+ VRGYVSC +GCP EG V  + VA VAK+LYSMGC EISLGDTIGV
Sbjct: 149 RFDGVMQAARAASISVRGYVSCALGCPYEGKVSPAKVAEVAKKLYSMGCYEISLGDTIGV 208

Query: 262 GTPGTVIPMLESVANVVPTENLAVHFHDTYGQALSNILVSLQMGIGAVDSSVSGLGGCPY 321
           GTPG +  ML +V + VP   LAVH HDTYGQAL+N LV+LQMG+  VDSSV+GLGGCPY
Sbjct: 209 GTPGLMKDMLTAVLHEVPVAALAVHCHDTYGQALANTLVALQMGVSVVDSSVAGLGGCPY 268

Query: 322 AKGASGNVATEDVVYMLNGIGAKTNVDLGKLMLAGDFICKQLGRPSASKVAIALRKV 379
           AKGASGN+ATED+VYML G+G  T V+L KL+ AGDFIC+ L R ++SKVA A  K+
Sbjct: 269 AKGASGNLATEDLVYMLTGLGIHTGVNLQKLLEAGDFICQALNRKTSSKVAQATCKL 325

BLAST of CsGy3G035930 vs. ExPASy Swiss-Prot
Match: Q8JZS7 (3-hydroxy-3-methylglutaryl-CoA lyase, cytoplasmic OS=Mus musculus OX=10090 GN=Hmgcll1 PE=2 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 2.1e-107
Identity = 194/293 (66.21%), Postives = 232/293 (79.18%), Query Frame = 0

Query: 82  IPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSGLPVVEATSFVSPKWVPQLADA 141
           +P +VKIVEVGPRDGLQNEK IVPT +K++LI  L  +GL V+E TSFVS +WVPQ+AD 
Sbjct: 44  LPEYVKIVEVGPRDGLQNEKVIVPTDIKIELINQLSQTGLSVIEVTSFVSSRWVPQMADH 103

Query: 142 KEVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIFASASESFSKSNINCSIEESLH 201
            EVM GIR   G R+PVLTPNL+GF+ AVAAGA E+A+F +ASESFSK NINCSIEES+ 
Sbjct: 104 AEVMRGIRQYPGVRYPVLTPNLQGFQHAVAAGATEIAVFGAASESFSKKNINCSIEESMG 163

Query: 202 RYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAYVAKELYSMGCSEISLGDTIGV 261
           R+++V  +AR   +PVRGYVSC +GCP EG +    V  V+K LY MGC EISLGDTIGV
Sbjct: 164 RFQEVISSARHMDIPVRGYVSCALGCPYEGSITPQKVTEVSKRLYGMGCYEISLGDTIGV 223

Query: 262 GTPGTVIPMLESVANVVPTENLAVHFHDTYGQALSNILVSLQMGIGAVDSSVSGLGGCPY 321
           GTPG++  MLESV   +P   LAVH HDTYGQAL+NIL +LQMGI  VDS+VSGLGGCPY
Sbjct: 224 GTPGSMKMMLESVMKEIPPGALAVHCHDTYGQALANILTALQMGINVVDSAVSGLGGCPY 283

Query: 322 AKGASGNVATEDVVYMLNGIGAKTNVDLGKLMLAGDFICKQLGRPSASKVAIA 375
           AKGASGNVATED++YMLNG+G  T VDL K+M AG+FICK + + + SKVA A
Sbjct: 284 AKGASGNVATEDLIYMLNGMGLNTGVDLYKVMEAGEFICKAVNKTTNSKVAQA 336

BLAST of CsGy3G035930 vs. ExPASy Swiss-Prot
Match: P38060 (Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Mus musculus OX=10090 GN=Hmgcl PE=1 SV=2)

HSP 1 Score: 389.0 bits (998), Expect = 6.2e-107
Identity = 197/297 (66.33%), Postives = 235/297 (79.12%), Query Frame = 0

Query: 82  IPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSGLPVVEATSFVSPKWVPQLADA 141
           +P  VKIVEVGPRDGLQNEK IVPT VK+ LI ML  +GLPV+EATSFVSPKWVPQ+AD 
Sbjct: 29  LPKQVKIVEVGPRDGLQNEKSIVPTPVKIRLIDMLSEAGLPVIEATSFVSPKWVPQMADH 88

Query: 142 KEVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIFASASESFSKSNINCSIEESLH 201
            +V++GI+   G  +PVLTPN+KGFE AVAAGAKEV++F + SE F++ N NCSIEES  
Sbjct: 89  SDVLKGIQKFPGINYPVLTPNMKGFEEAVAAGAKEVSVFGAVSELFTRKNANCSIEESFQ 148

Query: 202 RYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAYVAKELYSMGCSEISLGDTIGV 261
           R+  V  AA+  S+ VRGYVSC +GCP EG V  + VA VAK+LYSMGC EISLGDTIGV
Sbjct: 149 RFAGVMQAAQAASISVRGYVSCALGCPYEGKVSPAKVAEVAKKLYSMGCYEISLGDTIGV 208

Query: 262 GTPGTVIPMLESVANVVPTENLAVHFHDTYGQALSNILVSLQMGIGAVDSSVSGLGGCPY 321
           GTPG +  ML +V + VP   LAVH HDTYGQAL+N LV+LQMG+  VDSSV+GLGGCPY
Sbjct: 209 GTPGLMKDMLTAVMHEVPVTALAVHCHDTYGQALANTLVALQMGVSVVDSSVAGLGGCPY 268

Query: 322 AKGASGNVATEDVVYMLNGIGAKTNVDLGKLMLAGDFICKQLGRPSASKVAIALRKV 379
           AKGASGN+ATED+VYMLNG+G  T V+L KL+ AGDFIC+ L R ++SKVA A  K+
Sbjct: 269 AKGASGNLATEDLVYMLNGLGIHTGVNLQKLLEAGDFICQALNRKTSSKVAQATCKL 325

BLAST of CsGy3G035930 vs. NCBI nr
Match: XP_011652210.1 (hydroxymethylglutaryl-CoA lyase, mitochondrial [Cucumis sativus] >XP_011652211.1 hydroxymethylglutaryl-CoA lyase, mitochondrial [Cucumis sativus] >XP_031738871.1 hydroxymethylglutaryl-CoA lyase, mitochondrial [Cucumis sativus])

HSP 1 Score: 764 bits (1973), Expect = 3.39e-278
Identity = 391/392 (99.74%), Postives = 391/392 (99.74%), Query Frame = 0

Query: 1   MQAAKLSAKFTRQSFSWRHAYNLSMETPTPFLVSPSATDVGVSSIRTPGIQREFYNCSQQ 60
           MQAAKLSAKFTRQSFSWRHAYNLSMETPTPFLVSPSATDVGVSSIRTPGIQREFYNCSQQ
Sbjct: 1   MQAAKLSAKFTRQSFSWRHAYNLSMETPTPFLVSPSATDVGVSSIRTPGIQREFYNCSQQ 60

Query: 61  DGRDAVLSRENKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSG 120
           DGRDAVLSRENKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSG
Sbjct: 61  DGRDAVLSRENKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSG 120

Query: 121 LPVVEATSFVSPKWVPQLADAKEVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF 180
           LPVVEATSFVSPKWVPQLADAKEVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF
Sbjct: 121 LPVVEATSFVSPKWVPQLADAKEVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF 180

Query: 181 ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY 240
           ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY
Sbjct: 181 ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY 240

Query: 241 VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVANVVPTENLAVHFHDTYGQALSNILV 300
           VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVANVVPTENLAVHFHDTYGQALSNILV
Sbjct: 241 VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVANVVPTENLAVHFHDTYGQALSNILV 300

Query: 301 SLQMGIGAVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGAKTNVDLGKLMLAGDFIC 360
           SLQMGIG VDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGAKTNVDLGKLMLAGDFIC
Sbjct: 301 SLQMGIGTVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGAKTNVDLGKLMLAGDFIC 360

Query: 361 KQLGRPSASKVAIALRKVASNTNKCTATASKL 392
           KQLGRPSASKVAIALRKVASNTNKCTATASKL
Sbjct: 361 KQLGRPSASKVAIALRKVASNTNKCTATASKL 392

BLAST of CsGy3G035930 vs. NCBI nr
Match: KAE8651197.1 (hypothetical protein Csa_002494 [Cucumis sativus])

HSP 1 Score: 750 bits (1936), Expect = 3.93e-272
Identity = 391/418 (93.54%), Postives = 391/418 (93.54%), Query Frame = 0

Query: 1   MQAAKLSAKFTRQSFSWRHAYNLSMETPTPFLVSPSATDVGVSSIRTPGIQREFYNCSQQ 60
           MQAAKLSAKFTRQSFSWRHAYNLSMETPTPFLVSPSATDVGVSSIRTPGIQREFYNCSQQ
Sbjct: 1   MQAAKLSAKFTRQSFSWRHAYNLSMETPTPFLVSPSATDVGVSSIRTPGIQREFYNCSQQ 60

Query: 61  DGRDAVLSRENKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSG 120
           DGRDAVLSRENKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSG
Sbjct: 61  DGRDAVLSRENKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSG 120

Query: 121 LPVVEATSFVSPKWVPQL--------------------------ADAKEVMEGIRNVEGA 180
           LPVVEATSFVSPKWVPQL                          ADAKEVMEGIRNVEGA
Sbjct: 121 LPVVEATSFVSPKWVPQLPCLILPSPDLQLFMCSVFVFEFLVRLADAKEVMEGIRNVEGA 180

Query: 181 RFPVLTPNLKGFEAAVAAGAKEVAIFASASESFSKSNINCSIEESLHRYRQVADAARKHS 240
           RFPVLTPNLKGFEAAVAAGAKEVAIFASASESFSKSNINCSIEESLHRYRQVADAARKHS
Sbjct: 181 RFPVLTPNLKGFEAAVAAGAKEVAIFASASESFSKSNINCSIEESLHRYRQVADAARKHS 240

Query: 241 LPVRGYVSCVVGCPIEGMVPASNVAYVAKELYSMGCSEISLGDTIGVGTPGTVIPMLESV 300
           LPVRGYVSCVVGCPIEGMVPASNVAYVAKELYSMGCSEISLGDTIGVGTPGTVIPMLESV
Sbjct: 241 LPVRGYVSCVVGCPIEGMVPASNVAYVAKELYSMGCSEISLGDTIGVGTPGTVIPMLESV 300

Query: 301 ANVVPTENLAVHFHDTYGQALSNILVSLQMGIGAVDSSVSGLGGCPYAKGASGNVATEDV 360
           ANVVPTENLAVHFHDTYGQALSNILVSLQMGIG VDSSVSGLGGCPYAKGASGNVATEDV
Sbjct: 301 ANVVPTENLAVHFHDTYGQALSNILVSLQMGIGTVDSSVSGLGGCPYAKGASGNVATEDV 360

Query: 361 VYMLNGIGAKTNVDLGKLMLAGDFICKQLGRPSASKVAIALRKVASNTNKCTATASKL 392
           VYMLNGIGAKTNVDLGKLMLAGDFICKQLGRPSASKVAIALRKVASNTNKCTATASKL
Sbjct: 361 VYMLNGIGAKTNVDLGKLMLAGDFICKQLGRPSASKVAIALRKVASNTNKCTATASKL 418

BLAST of CsGy3G035930 vs. NCBI nr
Match: XP_008443338.1 (PREDICTED: hydroxymethylglutaryl-CoA lyase, mitochondrial-like [Cucumis melo] >KAA0053821.1 hydroxymethylglutaryl-CoA lyase [Cucumis melo var. makuwa] >TYK25580.1 hydroxymethylglutaryl-CoA lyase [Cucumis melo var. makuwa])

HSP 1 Score: 721 bits (1861), Expect = 3.42e-261
Identity = 373/392 (95.15%), Postives = 377/392 (96.17%), Query Frame = 0

Query: 1   MQAAKLSAKFTRQSFSWRHAYNLSMETPTPFLVSPSATDVGVSSIRTPGIQREFYNCSQQ 60
           MQAAKLSAKFTRQ+FSWRHAYNLSM+T    LVSPSA DVGV+SI TPGIQREFYNCSQQ
Sbjct: 1   MQAAKLSAKFTRQAFSWRHAYNLSMKT----LVSPSAADVGVNSITTPGIQREFYNCSQQ 60

Query: 61  DGRDAVLSRENKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSG 120
           DGRDAVLSRENKGGLSQ LCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLV SG
Sbjct: 61  DGRDAVLSRENKGGLSQNLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVFSG 120

Query: 121 LPVVEATSFVSPKWVPQLADAKEVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF 180
           LPVVEATSFVSPKWVPQLADAK+VMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF
Sbjct: 121 LPVVEATSFVSPKWVPQLADAKDVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF 180

Query: 181 ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY 240
           ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY
Sbjct: 181 ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY 240

Query: 241 VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVANVVPTENLAVHFHDTYGQALSNILV 300
           VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESV NVVP ENLAVHFHDTYGQALSNILV
Sbjct: 241 VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVINVVPIENLAVHFHDTYGQALSNILV 300

Query: 301 SLQMGIGAVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGAKTNVDLGKLMLAGDFIC 360
           SLQMGIG VDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGAKTNVDL KLMLAGDFIC
Sbjct: 301 SLQMGIGTVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGAKTNVDLEKLMLAGDFIC 360

Query: 361 KQLGRPSASKVAIALRKVASNTNKCTATASKL 392
           K LGRPSASKVAIALRKV SN NKCTATASKL
Sbjct: 361 KHLGRPSASKVAIALRKVTSNANKCTATASKL 388

BLAST of CsGy3G035930 vs. NCBI nr
Match: XP_038905646.1 (hydroxymethylglutaryl-CoA lyase, mitochondrial-like [Benincasa hispida])

HSP 1 Score: 704 bits (1817), Expect = 2.01e-254
Identity = 362/392 (92.35%), Postives = 371/392 (94.64%), Query Frame = 0

Query: 1   MQAAKLSAKFTRQSFSWRHAYNLSMETPTPFLVSPSATDVGVSSIRTPGIQREFYNCSQQ 60
           MQ AKLSAK TRQ FSWR+AYNLSMETP  FLV P ATDVGV+SIR  GI+RE  NCSQQ
Sbjct: 1   MQTAKLSAKLTRQGFSWRYAYNLSMETPCRFLVPPLATDVGVNSIRRLGIRREVGNCSQQ 60

Query: 61  DGRDAVLSRENKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSG 120
           DGRDAVLSRENKGGLSQYLC IP FVKIVEVGPRDGLQNEKHIVPTAVKV+LIKMLVSSG
Sbjct: 61  DGRDAVLSRENKGGLSQYLCHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSG 120

Query: 121 LPVVEATSFVSPKWVPQLADAKEVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF 180
           LP+VEATSFVSPKWVPQLADAK+VMEGI+NVEGARFPVLTPNLKGFEAAVAAGAKEVAIF
Sbjct: 121 LPIVEATSFVSPKWVPQLADAKDVMEGIQNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF 180

Query: 181 ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY 240
           ASASESFSKSNINCSIEESLHRYR+VADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY
Sbjct: 181 ASASESFSKSNINCSIEESLHRYREVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY 240

Query: 241 VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVANVVPTENLAVHFHDTYGQALSNILV 300
           VAKELY MGCSEISLGDTIGVGTPGTVIPMLESV NVVP ENLAVHFHDTYGQALSNILV
Sbjct: 241 VAKELYRMGCSEISLGDTIGVGTPGTVIPMLESVVNVVPIENLAVHFHDTYGQALSNILV 300

Query: 301 SLQMGIGAVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGAKTNVDLGKLMLAGDFIC 360
           SLQMGIG VDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIG KT+VDL KLMLAGDFIC
Sbjct: 301 SLQMGIGTVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTDVDLEKLMLAGDFIC 360

Query: 361 KQLGRPSASKVAIALRKVASNTNKCTATASKL 392
           K LGRPSASKVAIALRKVASN NKCTATASKL
Sbjct: 361 KHLGRPSASKVAIALRKVASNANKCTATASKL 392

BLAST of CsGy3G035930 vs. NCBI nr
Match: XP_023526450.1 (hydroxymethylglutaryl-CoA lyase, mitochondrial-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023526451.1 hydroxymethylglutaryl-CoA lyase, mitochondrial-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 642 bits (1657), Expect = 4.73e-230
Identity = 337/392 (85.97%), Postives = 351/392 (89.54%), Query Frame = 0

Query: 1   MQAAKLSAKFTRQSFSWRHAYNLSMETPTPFLVSPSATDVGVSSIRTPGIQREFYNCSQQ 60
           MQAAK+SAK TR+  SWRH YN +M   + FL S SA+ VGV+S    GIQ E  NC QQ
Sbjct: 1   MQAAKVSAKLTRRGLSWRHGYNPTMRISSRFLSSSSASHVGVTSTNGLGIQGEVDNCCQQ 60

Query: 61  DGRDAVLSRENKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSG 120
           DG DAVLSRENK  LSQYL  IP FVKIVEVGPRDGLQNEKHIVPTAVKV+LIKMLVSSG
Sbjct: 61  DGGDAVLSRENKRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSG 120

Query: 121 LPVVEATSFVSPKWVPQLADAKEVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF 180
           L +VEATSFVSPKWVPQLADA +VMEGIRNVEGARFPVLTPN+KGFEAAVAAGAKEVAIF
Sbjct: 121 LRIVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIF 180

Query: 181 ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY 240
           ASASESFSKSNINCSIEESL RYR+VA AARKHSLPVRGYVSCVVGCPIEGMVP SNVAY
Sbjct: 181 ASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNVAY 240

Query: 241 VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVANVVPTENLAVHFHDTYGQALSNILV 300
           VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESV +VVP ENLAVHFHDTYGQALSNIL+
Sbjct: 241 VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILL 300

Query: 301 SLQMGIGAVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGAKTNVDLGKLMLAGDFIC 360
           SLQMGIG VD SVSGLGGCPYAKGASGNVATEDVVYMLNGIG KTNVDL KLMLAGDFI 
Sbjct: 301 SLQMGIGTVDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFIS 360

Query: 361 KQLGRPSASKVAIALRKVASNTNKCTATASKL 392
           K LGRPSASKVAIAL+KVAS  NKCTATASKL
Sbjct: 361 KHLGRPSASKVAIALQKVASKPNKCTATASKL 392

BLAST of CsGy3G035930 vs. ExPASy TrEMBL
Match: A0A0A0LHM6 (Hydroxymethylglutaryl-CoA lyase OS=Cucumis sativus OX=3659 GN=Csa_3G823050 PE=3 SV=1)

HSP 1 Score: 764 bits (1973), Expect = 1.64e-278
Identity = 391/392 (99.74%), Postives = 391/392 (99.74%), Query Frame = 0

Query: 1   MQAAKLSAKFTRQSFSWRHAYNLSMETPTPFLVSPSATDVGVSSIRTPGIQREFYNCSQQ 60
           MQAAKLSAKFTRQSFSWRHAYNLSMETPTPFLVSPSATDVGVSSIRTPGIQREFYNCSQQ
Sbjct: 1   MQAAKLSAKFTRQSFSWRHAYNLSMETPTPFLVSPSATDVGVSSIRTPGIQREFYNCSQQ 60

Query: 61  DGRDAVLSRENKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSG 120
           DGRDAVLSRENKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSG
Sbjct: 61  DGRDAVLSRENKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSG 120

Query: 121 LPVVEATSFVSPKWVPQLADAKEVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF 180
           LPVVEATSFVSPKWVPQLADAKEVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF
Sbjct: 121 LPVVEATSFVSPKWVPQLADAKEVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF 180

Query: 181 ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY 240
           ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY
Sbjct: 181 ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY 240

Query: 241 VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVANVVPTENLAVHFHDTYGQALSNILV 300
           VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVANVVPTENLAVHFHDTYGQALSNILV
Sbjct: 241 VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVANVVPTENLAVHFHDTYGQALSNILV 300

Query: 301 SLQMGIGAVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGAKTNVDLGKLMLAGDFIC 360
           SLQMGIG VDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGAKTNVDLGKLMLAGDFIC
Sbjct: 301 SLQMGIGTVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGAKTNVDLGKLMLAGDFIC 360

Query: 361 KQLGRPSASKVAIALRKVASNTNKCTATASKL 392
           KQLGRPSASKVAIALRKVASNTNKCTATASKL
Sbjct: 361 KQLGRPSASKVAIALRKVASNTNKCTATASKL 392

BLAST of CsGy3G035930 vs. ExPASy TrEMBL
Match: A0A5A7UJY0 (Hydroxymethylglutaryl-CoA lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G007370 PE=3 SV=1)

HSP 1 Score: 721 bits (1861), Expect = 1.66e-261
Identity = 373/392 (95.15%), Postives = 377/392 (96.17%), Query Frame = 0

Query: 1   MQAAKLSAKFTRQSFSWRHAYNLSMETPTPFLVSPSATDVGVSSIRTPGIQREFYNCSQQ 60
           MQAAKLSAKFTRQ+FSWRHAYNLSM+T    LVSPSA DVGV+SI TPGIQREFYNCSQQ
Sbjct: 1   MQAAKLSAKFTRQAFSWRHAYNLSMKT----LVSPSAADVGVNSITTPGIQREFYNCSQQ 60

Query: 61  DGRDAVLSRENKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSG 120
           DGRDAVLSRENKGGLSQ LCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLV SG
Sbjct: 61  DGRDAVLSRENKGGLSQNLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVFSG 120

Query: 121 LPVVEATSFVSPKWVPQLADAKEVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF 180
           LPVVEATSFVSPKWVPQLADAK+VMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF
Sbjct: 121 LPVVEATSFVSPKWVPQLADAKDVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF 180

Query: 181 ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY 240
           ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY
Sbjct: 181 ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY 240

Query: 241 VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVANVVPTENLAVHFHDTYGQALSNILV 300
           VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESV NVVP ENLAVHFHDTYGQALSNILV
Sbjct: 241 VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVINVVPIENLAVHFHDTYGQALSNILV 300

Query: 301 SLQMGIGAVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGAKTNVDLGKLMLAGDFIC 360
           SLQMGIG VDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGAKTNVDL KLMLAGDFIC
Sbjct: 301 SLQMGIGTVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGAKTNVDLEKLMLAGDFIC 360

Query: 361 KQLGRPSASKVAIALRKVASNTNKCTATASKL 392
           K LGRPSASKVAIALRKV SN NKCTATASKL
Sbjct: 361 KHLGRPSASKVAIALRKVTSNANKCTATASKL 388

BLAST of CsGy3G035930 vs. ExPASy TrEMBL
Match: A0A1S3B7B2 (Hydroxymethylglutaryl-CoA lyase OS=Cucumis melo OX=3656 GN=LOC103486948 PE=3 SV=1)

HSP 1 Score: 721 bits (1861), Expect = 1.66e-261
Identity = 373/392 (95.15%), Postives = 377/392 (96.17%), Query Frame = 0

Query: 1   MQAAKLSAKFTRQSFSWRHAYNLSMETPTPFLVSPSATDVGVSSIRTPGIQREFYNCSQQ 60
           MQAAKLSAKFTRQ+FSWRHAYNLSM+T    LVSPSA DVGV+SI TPGIQREFYNCSQQ
Sbjct: 1   MQAAKLSAKFTRQAFSWRHAYNLSMKT----LVSPSAADVGVNSITTPGIQREFYNCSQQ 60

Query: 61  DGRDAVLSRENKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSG 120
           DGRDAVLSRENKGGLSQ LCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLV SG
Sbjct: 61  DGRDAVLSRENKGGLSQNLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVFSG 120

Query: 121 LPVVEATSFVSPKWVPQLADAKEVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF 180
           LPVVEATSFVSPKWVPQLADAK+VMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF
Sbjct: 121 LPVVEATSFVSPKWVPQLADAKDVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF 180

Query: 181 ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY 240
           ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY
Sbjct: 181 ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY 240

Query: 241 VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVANVVPTENLAVHFHDTYGQALSNILV 300
           VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESV NVVP ENLAVHFHDTYGQALSNILV
Sbjct: 241 VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVINVVPIENLAVHFHDTYGQALSNILV 300

Query: 301 SLQMGIGAVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGAKTNVDLGKLMLAGDFIC 360
           SLQMGIG VDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGAKTNVDL KLMLAGDFIC
Sbjct: 301 SLQMGIGTVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGAKTNVDLEKLMLAGDFIC 360

Query: 361 KQLGRPSASKVAIALRKVASNTNKCTATASKL 392
           K LGRPSASKVAIALRKV SN NKCTATASKL
Sbjct: 361 KHLGRPSASKVAIALRKVTSNANKCTATASKL 388

BLAST of CsGy3G035930 vs. ExPASy TrEMBL
Match: A0A6J1J8Q9 (Hydroxymethylglutaryl-CoA lyase OS=Cucurbita maxima OX=3661 GN=LOC111482306 PE=3 SV=1)

HSP 1 Score: 642 bits (1657), Expect = 2.38e-230
Identity = 337/392 (85.97%), Postives = 351/392 (89.54%), Query Frame = 0

Query: 1   MQAAKLSAKFTRQSFSWRHAYNLSMETPTPFLVSPSATDVGVSSIRTPGIQREFYNCSQQ 60
           MQAAK+SAK TR+  SWRH YN +M   + FL S SA+ VGVSS    GIQ E +NC QQ
Sbjct: 2   MQAAKVSAKLTRRGLSWRHGYNPTMRISSRFLSSSSASHVGVSSTNGLGIQGEVHNCCQQ 61

Query: 61  DGRDAVLSRENKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSG 120
           DG DAVLSRENK  LSQYL  IP FVKIVEVGPRDGLQNEKHIVPTAVKV+LIKMLVSSG
Sbjct: 62  DGGDAVLSRENKRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSG 121

Query: 121 LPVVEATSFVSPKWVPQLADAKEVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF 180
           L +VEATSFVSPKWVPQLADA +VMEGIRNVEGARFPVLTPN+KGFEAAVAAGAKEVAIF
Sbjct: 122 LRIVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIF 181

Query: 181 ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY 240
           ASASESFSKSNINCSIEESL RYR+VA AARK SLPVRGYVSCVVGCPIEGMVP SNVAY
Sbjct: 182 ASASESFSKSNINCSIEESLRRYREVAVAARKQSLPVRGYVSCVVGCPIEGMVPPSNVAY 241

Query: 241 VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVANVVPTENLAVHFHDTYGQALSNILV 300
           VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESV +VVP ENLAVHFHDTYGQALSNIL+
Sbjct: 242 VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILL 301

Query: 301 SLQMGIGAVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGAKTNVDLGKLMLAGDFIC 360
           SLQMGIG VD SVSGLGGCPYAKGASGNVATEDVVYMLNGIG KTNVDL KLMLAGDFI 
Sbjct: 302 SLQMGIGTVDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFIS 361

Query: 361 KQLGRPSASKVAIALRKVASNTNKCTATASKL 392
           K LGRPSASKVAIAL+KVAS  NKCTATASKL
Sbjct: 362 KHLGRPSASKVAIALQKVASKPNKCTATASKL 393

BLAST of CsGy3G035930 vs. ExPASy TrEMBL
Match: A0A6J1F7V0 (Hydroxymethylglutaryl-CoA lyase OS=Cucurbita moschata OX=3662 GN=LOC111441653 PE=3 SV=1)

HSP 1 Score: 641 bits (1653), Expect = 9.32e-230
Identity = 337/392 (85.97%), Postives = 349/392 (89.03%), Query Frame = 0

Query: 1   MQAAKLSAKFTRQSFSWRHAYNLSMETPTPFLVSPSATDVGVSSIRTPGIQREFYNCSQQ 60
           MQAAK+SAK TR+  SWRH YN +M   + FL S SA+ VGVSS    GIQ E  NC QQ
Sbjct: 1   MQAAKVSAKLTRRGLSWRHGYNPTMRISSRFLSSSSASHVGVSSTNGLGIQGEVDNCCQQ 60

Query: 61  DGRDAVLSRENKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSG 120
           DG D VLSRENK  LSQYL  IP FVKIVEVGPRDGLQNEKHIVPTAVKV+LIKMLVSSG
Sbjct: 61  DGGDVVLSRENKRRLSQYLRHIPQFVKIVEVGPRDGLQNEKHIVPTAVKVELIKMLVSSG 120

Query: 121 LPVVEATSFVSPKWVPQLADAKEVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIF 180
           L VVEATSFVSPKWVPQLADA +VMEGIRNVEGARFPVLTPN+KGFEAAVAAGAKEVAIF
Sbjct: 121 LRVVEATSFVSPKWVPQLADANDVMEGIRNVEGARFPVLTPNIKGFEAAVAAGAKEVAIF 180

Query: 181 ASASESFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAY 240
           ASASESFSKSNINCSIEESL RYR+VA AARKHSLPVRGYVSCVVGCPIEGMVP SNVAY
Sbjct: 181 ASASESFSKSNINCSIEESLRRYREVAVAARKHSLPVRGYVSCVVGCPIEGMVPPSNVAY 240

Query: 241 VAKELYSMGCSEISLGDTIGVGTPGTVIPMLESVANVVPTENLAVHFHDTYGQALSNILV 300
           VAKELY MGCSEISLGDTIGVGTPGTVIPMLESV +VVP ENLAVHFHDTYGQALSNIL+
Sbjct: 241 VAKELYGMGCSEISLGDTIGVGTPGTVIPMLESVVDVVPIENLAVHFHDTYGQALSNILL 300

Query: 301 SLQMGIGAVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGAKTNVDLGKLMLAGDFIC 360
           SLQMGIG VD SVSGLGGCPYAKGASGNVATEDVVYMLNGIG KTNVDL KLMLAGDFI 
Sbjct: 301 SLQMGIGTVDCSVSGLGGCPYAKGASGNVATEDVVYMLNGIGVKTNVDLEKLMLAGDFIS 360

Query: 361 KQLGRPSASKVAIALRKVASNTNKCTATASKL 392
           K LGRPSASKVAIAL+KVAS  NKCTATASKL
Sbjct: 361 KHLGRPSASKVAIALQKVASKPNKCTATASKL 392

BLAST of CsGy3G035930 vs. TAIR 10
Match: AT2G26800.1 (Aldolase superfamily protein )

HSP 1 Score: 479.6 bits (1233), Expect = 2.5e-135
Identity = 254/387 (65.63%), Postives = 299/387 (77.26%), Query Frame = 0

Query: 10  FTRQSFSWRHAYNLSMETPTPF----LVSPSATDVGVSSIRTPGIQREFYNCSQQDGRDA 69
           F ++SF WR       E    F        ++T  G         ++++   S ++G   
Sbjct: 61  FAKESFPWRRHTRKLSEGEHMFRNISFAGRTSTVSGTLRESKSFKEQKYSTFSNENGTSH 120

Query: 70  VLSRENKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSGLPVVE 129
           + ++ +KG        IP FVKIVEVGPRDGLQNEK+IVPT+VKV+LI+ LVSSGLPVVE
Sbjct: 121 ISNKISKG--------IPKFVKIVEVGPRDGLQNEKNIVPTSVKVELIQRLVSSGLPVVE 180

Query: 130 ATSFVSPKWVPQLADAKEVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIFASASE 189
           ATSFVSPKWVPQLADAK+VM+ +  ++GAR PVLTPNLKGF+AAV+AGAKEVAIFASASE
Sbjct: 181 ATSFVSPKWVPQLADAKDVMDAVNTLDGARLPVLTPNLKGFQAAVSAGAKEVAIFASASE 240

Query: 190 SFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAYVAKEL 249
           SFS SNINC+IEESL RYR VA AA++HS+PVRGYVSCVVGCP+EG V  S VAYV KEL
Sbjct: 241 SFSLSNINCTIEESLLRYRVVATAAKEHSVPVRGYVSCVVGCPVEGPVLPSKVAYVVKEL 300

Query: 250 YSMGCSEISLGDTIGVGTPGTVIPMLESVANVVPTENLAVHFHDTYGQALSNILVSLQMG 309
           Y MGC EISLGDTIG+GTPG+V+PMLE+V  VVP + LAVHFHDTYGQAL+NILVSLQMG
Sbjct: 301 YDMGCFEISLGDTIGIGTPGSVVPMLEAVMAVVPADKLAVHFHDTYGQALANILVSLQMG 360

Query: 310 IGAVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGAKTNVDLGKLMLAGDFICKQLGR 369
           I  VDSS++GLGGCPYAKGASGNVATEDVVYMLNG+G  TNVDLGKL+ AGDFI K LGR
Sbjct: 361 ISIVDSSIAGLGGCPYAKGASGNVATEDVVYMLNGLGVHTNVDLGKLIAAGDFISKHLGR 420

Query: 370 PSASKVAIALRKVASNTNKCTATASKL 393
           P+ SK A+AL +      + TA ASK+
Sbjct: 421 PNGSKAAVALNR------RITADASKI 433

BLAST of CsGy3G035930 vs. TAIR 10
Match: AT2G26800.2 (Aldolase superfamily protein )

HSP 1 Score: 479.6 bits (1233), Expect = 2.5e-135
Identity = 254/387 (65.63%), Postives = 299/387 (77.26%), Query Frame = 0

Query: 10  FTRQSFSWRHAYNLSMETPTPF----LVSPSATDVGVSSIRTPGIQREFYNCSQQDGRDA 69
           F ++SF WR       E    F        ++T  G         ++++   S ++G   
Sbjct: 96  FAKESFPWRRHTRKLSEGEHMFRNISFAGRTSTVSGTLRESKSFKEQKYSTFSNENGTSH 155

Query: 70  VLSRENKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSGLPVVE 129
           + ++ +KG        IP FVKIVEVGPRDGLQNEK+IVPT+VKV+LI+ LVSSGLPVVE
Sbjct: 156 ISNKISKG--------IPKFVKIVEVGPRDGLQNEKNIVPTSVKVELIQRLVSSGLPVVE 215

Query: 130 ATSFVSPKWVPQLADAKEVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIFASASE 189
           ATSFVSPKWVPQLADAK+VM+ +  ++GAR PVLTPNLKGF+AAV+AGAKEVAIFASASE
Sbjct: 216 ATSFVSPKWVPQLADAKDVMDAVNTLDGARLPVLTPNLKGFQAAVSAGAKEVAIFASASE 275

Query: 190 SFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAYVAKEL 249
           SFS SNINC+IEESL RYR VA AA++HS+PVRGYVSCVVGCP+EG V  S VAYV KEL
Sbjct: 276 SFSLSNINCTIEESLLRYRVVATAAKEHSVPVRGYVSCVVGCPVEGPVLPSKVAYVVKEL 335

Query: 250 YSMGCSEISLGDTIGVGTPGTVIPMLESVANVVPTENLAVHFHDTYGQALSNILVSLQMG 309
           Y MGC EISLGDTIG+GTPG+V+PMLE+V  VVP + LAVHFHDTYGQAL+NILVSLQMG
Sbjct: 336 YDMGCFEISLGDTIGIGTPGSVVPMLEAVMAVVPADKLAVHFHDTYGQALANILVSLQMG 395

Query: 310 IGAVDSSVSGLGGCPYAKGASGNVATEDVVYMLNGIGAKTNVDLGKLMLAGDFICKQLGR 369
           I  VDSS++GLGGCPYAKGASGNVATEDVVYMLNG+G  TNVDLGKL+ AGDFI K LGR
Sbjct: 396 ISIVDSSIAGLGGCPYAKGASGNVATEDVVYMLNGLGVHTNVDLGKLIAAGDFISKHLGR 455

Query: 370 PSASKVAIALRKVASNTNKCTATASKL 393
           P+ SK A+AL +      + TA ASK+
Sbjct: 456 PNGSKAAVALNR------RITADASKI 468

BLAST of CsGy3G035930 vs. TAIR 10
Match: AT2G26800.3 (Aldolase superfamily protein )

HSP 1 Score: 305.8 bits (782), Expect = 4.9e-83
Identity = 163/263 (61.98%), Postives = 195/263 (74.14%), Query Frame = 0

Query: 10  FTRQSFSWRHAYNLSMETPTPF----LVSPSATDVGVSSIRTPGIQREFYNCSQQDGRDA 69
           F ++SF WR       E    F        ++T  G         ++++   S ++G   
Sbjct: 96  FAKESFPWRRHTRKLSEGEHMFRNISFAGRTSTVSGTLRESKSFKEQKYSTFSNENGTSH 155

Query: 70  VLSRENKGGLSQYLCRIPPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSGLPVVE 129
           + ++ +KG        IP FVKIVEVGPRDGLQNEK+IVPT+VKV+LI+ LVSSGLPVVE
Sbjct: 156 ISNKISKG--------IPKFVKIVEVGPRDGLQNEKNIVPTSVKVELIQRLVSSGLPVVE 215

Query: 130 ATSFVSPKWVPQLADAKEVMEGIRNVEGARFPVLTPNLKGFEAAVAAGAKEVAIFASASE 189
           ATSFVSPKWVPQLADAK+VM+ +  ++GAR PVLTPNLKGF+AAV+AGAKEVAIFASASE
Sbjct: 216 ATSFVSPKWVPQLADAKDVMDAVNTLDGARLPVLTPNLKGFQAAVSAGAKEVAIFASASE 275

Query: 190 SFSKSNINCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAYVAKEL 249
           SFS SNINC+IEESL RYR VA AA++HS+PVRGYVSCVVGCP+EG V  S VAYV KEL
Sbjct: 276 SFSLSNINCTIEESLLRYRVVATAAKEHSVPVRGYVSCVVGCPVEGPVLPSKVAYVVKEL 335

Query: 250 YSMGCSEISLGDTIGVGTPGTVI 269
           Y MGC EISLGDTIG+GTPG  +
Sbjct: 336 YDMGCFEISLGDTIGIGTPGNFV 350

BLAST of CsGy3G035930 vs. TAIR 10
Match: AT1G74040.1 (2-isopropylmalate synthase 1 )

HSP 1 Score: 45.4 bits (106), Expect = 1.2e-04
Identity = 58/266 (21.80%), Postives = 107/266 (40.23%), Query Frame = 0

Query: 83  PPFVKIVEVGPRDGLQNEKHIVPTAVKVDLIKMLVSSGLPVVEATSFVSPKWVPQLADAK 142
           P +V+I +   RDG Q+    + +  K+D+ + L   G+ ++EA    + K        K
Sbjct: 84  PNYVRIFDTTLRDGEQSPGATLTSKEKLDIARQLAKLGVDIIEAGFPAASK--DDFEAVK 143

Query: 143 EVMEGIRNV--EGARFPVLTP----NLK----GFEAAVAAGAKEVAIFASASESFSKSNI 202
            + E + N   E    PV+      N K     +EA   A    +  F + S+   K  +
Sbjct: 144 TIAETVGNTVDENGYVPVICGLSRCNKKDIETAWEAVKYAKRPRIHTFIATSDIHLKYKL 203

Query: 203 NCSIEESLHRYRQVADAARKHSLPVRGYVSCVVGCPIEGMVPASNVAYVAKELYSMGCSE 262
             S EE +   R +   AR       G           G      +  +  E+   G + 
Sbjct: 204 KKSKEEVIEIARNMVRFARS-----LGCEDVEFSPEDAGRSEREYLYEILGEVIKAGATT 263

Query: 263 ISLGDTIGVGTPGTVIPMLESV-ANVVPTEN--LAVHFHDTYGQALSNILVSLQMGIGAV 322
           +++ DT+G+  P     ++  + AN    +N  ++ H  +  G + +N L     G   V
Sbjct: 264 LNIPDTVGITLPSEFGQLIADIKANTPGIQNVIISTHCQNDLGLSTANTLSGAHSGARQV 323

Query: 323 DSSVSGLGGCPYAKGASGNVATEDVV 336
           + +++G+G        +GN + E+VV
Sbjct: 324 EVTINGIG------ERAGNASLEEVV 336

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O810273.5e-13465.63Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Arabidopsis thaliana OX=3702 G... [more]
P359153.0e-10968.15Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Gallus gallus OX=9031 GN=HMGCL... [more]
P975199.6e-10867.00Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Rattus norvegicus OX=10116 GN=... [more]
Q8JZS72.1e-10766.213-hydroxy-3-methylglutaryl-CoA lyase, cytoplasmic OS=Mus musculus OX=10090 GN=Hm... [more]
P380606.2e-10766.33Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Mus musculus OX=10090 GN=Hmgcl... [more]
Match NameE-valueIdentityDescription
XP_011652210.13.39e-27899.74hydroxymethylglutaryl-CoA lyase, mitochondrial [Cucumis sativus] >XP_011652211.1... [more]
KAE8651197.13.93e-27293.54hypothetical protein Csa_002494 [Cucumis sativus][more]
XP_008443338.13.42e-26195.15PREDICTED: hydroxymethylglutaryl-CoA lyase, mitochondrial-like [Cucumis melo] >K... [more]
XP_038905646.12.01e-25492.35hydroxymethylglutaryl-CoA lyase, mitochondrial-like [Benincasa hispida][more]
XP_023526450.14.73e-23085.97hydroxymethylglutaryl-CoA lyase, mitochondrial-like isoform X1 [Cucurbita pepo s... [more]
Match NameE-valueIdentityDescription
A0A0A0LHM61.64e-27899.74Hydroxymethylglutaryl-CoA lyase OS=Cucumis sativus OX=3659 GN=Csa_3G823050 PE=3 ... [more]
A0A5A7UJY01.66e-26195.15Hydroxymethylglutaryl-CoA lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A1S3B7B21.66e-26195.15Hydroxymethylglutaryl-CoA lyase OS=Cucumis melo OX=3656 GN=LOC103486948 PE=3 SV=... [more]
A0A6J1J8Q92.38e-23085.97Hydroxymethylglutaryl-CoA lyase OS=Cucurbita maxima OX=3661 GN=LOC111482306 PE=3... [more]
A0A6J1F7V09.32e-23085.97Hydroxymethylglutaryl-CoA lyase OS=Cucurbita moschata OX=3662 GN=LOC111441653 PE... [more]
Match NameE-valueIdentityDescription
AT2G26800.12.5e-13565.63Aldolase superfamily protein [more]
AT2G26800.22.5e-13565.63Aldolase superfamily protein [more]
AT2G26800.34.9e-8361.98Aldolase superfamily protein [more]
AT1G74040.11.2e-0421.802-isopropylmalate synthase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000891Pyruvate carboxyltransferasePFAMPF00682HMGL-likecoord: 86..358
e-value: 4.8E-55
score: 186.9
IPR000891Pyruvate carboxyltransferasePROSITEPS50991PYR_CTcoord: 86..353
score: 34.838024
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 80..378
e-value: 4.2E-113
score: 379.6
IPR043594HMG-CoA lyasePANTHERPTHR42738HYDROXYMETHYLGLUTARYL-COA LYASEcoord: 17..384
NoneNo IPR availablePANTHERPTHR42738:SF15HYDROXYMETHYLGLUTARYL-COA LYASEcoord: 17..384
NoneNo IPR availableCDDcd07938DRE_TIM_HMGLcoord: 88..361
e-value: 1.45658E-172
score: 480.352
NoneNo IPR availableSUPERFAMILY51569Aldolasecoord: 81..376

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy3G035930.2CsGy3G035930.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046951 ketone body biosynthetic process
biological_process GO:0006552 leucine catabolic process
biological_process GO:0006629 lipid metabolic process
molecular_function GO:0004419 hydroxymethylglutaryl-CoA lyase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0016833 oxo-acid-lyase activity